Traditional Chinese medicine composition for relieving heat stress of animals as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for relieving animal heat stress and a preparation method and application thereof. The composition is prepared from talc, herba elsholtziae, radix scutellariae, rhizoma phragmitis, radix scrophulariae, radix trichosanthis, fructus gardeniae, semen coicis, pericarpium citri reticulatae and liquorice. According to a traditional Chinese medicine compatibility theory, the ten medicinal materials are reasonably combined according to a certain proportion, the obtained composition can effectively relieve heat stress reaction of animals caused by a high-temperature environment, the lactation level of dairy cows is remarkably improved, the milk quality is improved, good adaptability is shown in practical application, the composition is suitable for various breeding scenes, and the breeding efficiency is improved. And a reliable guarantee is provided for animal health.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, and in particular to a traditional Chinese medicine composition for relieving heat stress in animals, its preparation method, and its application. Background Technology

[0002] Dairy cows, due to their large size, have a relatively small skin area per kilogram of body weight for heat dissipation; their sweat glands are underdeveloped, resulting in low heat dissipation efficiency; and their thick subcutaneous fat layer makes it easy for heat to accumulate in their bodies. 16-20℃ and 50%–70% RH are the comfortable range for lactating dairy cows. When temperature and relative humidity rise, and the THI exceeds 72, dairy cows cannot maintain a balance between heat accumulation and heat dissipation. This leads to heat stress, characterized by increased body temperature, accelerated breathing, decreased appetite, reduced dry matter intake, decreased conversion efficiency, decreased milk production, reduced fertility, and increased morbidity and mortality. Ultimately, this negatively impacts the economic benefits of dairy farming and increases breeding costs.

[0003] To mitigate the effects of heat stress on dairy cows, large-scale dairy farms often implement cooling plans primarily based on spraying, supplemented by fans for environmental control. While this cooling method is effective, its water resource utilization needs improvement, and the increased humidity in the cowsheds leads to a higher incidence of mastitis, hoof diseases, and other ailments in dairy cows. Therefore, adding various substances with heat stress-relieving properties to the feed of dairy cows is an important strategy for alleviating heat stress, including minerals, vitamins, microbial preparations, and traditional Chinese medicine ingredients. However, various chemical agents pose risks of drug resistance and drug-induced diseases; therefore, developing a residue-free, fast-acting, and highly safe pure traditional Chinese medicine preparation is an urgent need in the dairy industry.

[0004] The formula disclosed in patent CN 108704078 consists of 16 medicinal herbs: patchouli, perilla leaf, tangerine peel, magnolia bark, angelica root, poria cocos, talc, astragalus root, raw rhubarb, raw gypsum, anemarrhena rhizome, reed rhizome, apricot kernel, houttuynia cordata, trichosanthes fruit, and licorice root. It is produced using either a "whole powder" (passing through a 120-mesh sieve) or "added later in decoction" process (specific herbs are decocted with other herbs for a period before being added to the pot to retain their active ingredients). The combined herbs are used to regulate qi and strengthen the spleen, clear heat and generate fluids, dispel dampness and purge fire, harmonize the stomach and stop vomiting, promote urination and relieve strangury, and moisten the intestines and relieve constipation. Experimental data shows that this composition can effectively reduce the body temperature of heat-stressed cattle, slow respiratory rate, increase feed intake, enhance immunity, and maintain water and electrolyte balance, effectively alleviating and treating acute heat stress symptoms in dairy cows. However, the formula contains many medicinal ingredients and the preparation process is relatively complex, requiring high-level equipment and technology. The sources of medicinal materials such as Angelica dahurica and apricot kernels are limited, resulting in poor palatability and high costs, making it difficult to widely apply in aquaculture scenarios. Furthermore, the experimental observation period is only 48 hours, which is a short cycle. In actual production, the hot and humid weather in southern my country lasts for 5 to 6 months. The patented formula is cold in nature and has excessive purgation, posing significant safety risks if used for a long period of time.

[0005] Patent CN 108434393 A discloses a traditional Chinese medicine composition and preparation method for treating summer-dampness syndrome in human clinical practice. The composition consists of 21 medicinal herbs, including Elsholtzia ciliata and Forsythia suspensa, and is prepared as a decoction and pills. Clinical studies have shown that this formula has a short treatment time, good efficacy, and high cure rate for summer-dampness syndrome. However, its direct application to alleviating heat stress in dairy cows has significant limitations: First, the composition is primarily designed for human physiological and pathological characteristics, which differs from the heat stress relief needs of dairy cows as ruminants; second, the formula is complex and its preparation is cumbersome, making it difficult to meet the efficiency and economic requirements of large-scale farming; more importantly, the cyanogenic glycosides contained in herbs such as bitter almonds may pose a residual risk, potentially threatening the health of dairy cows and food safety.

[0006] To address the above deficiencies, this invention provides a composition that, through a "low-cost, long-cycle, and conditioning" approach, alleviates heat stress in dairy cows caused by high-temperature environments, intercepts heat stress damage at the "subclinical-metabolic disorder" stage, thereby reducing the incidence of mastitis, maintaining peak milk production, and providing reliable protection for healthy dairy farming. It is also more suitable as a daily supplement strategy during the high-temperature season. Summary of the Invention

[0007] In view of this, the present invention provides a traditional Chinese medicine composition for relieving heat stress in animals, its preparation method and application.

[0008] The traditional Chinese medicine composition provided by this invention comprises the following raw materials in parts by weight: Talc 20-40 parts, Elsholtzia ciliata 20-30 parts, Scutellaria baicalensis 5-10 parts, Phragmites communis 10-20 parts, Scrophularia ningpoensis 10-20 parts, Trichosanthes kirilowii 10-20 parts, Gardenia jasminoides 10-20 parts, Coix lacryma-jobi 20-30 parts, Citrus reticulata 5-20 parts, and Glycyrrhiza uralensis 5-20 parts.

[0009] Based on Traditional Chinese Medicine (TCM) theory, this invention targets the early and pre-stage heat stress in dairy cows. Combining animal physiological characteristics, and through in-depth research, screening, and scientific formulation, a TCM composition has been obtained, consisting of talc, *Elsholtzia ciliata*, *Scutellaria baicalensis*, *Phragmites communis*, *Scrophularia ningpoensis*, *Trichosanthes kirilowii*, *Gardenia jasminoides*, *Coix lacryma-jobi*, tangerine peel, and licorice. This composition primarily functions to "clear heat and lower body temperature, promote diuresis and eliminate dampness, and strengthen the spleen and stomach," while also possessing antioxidant, anti-inflammatory, and immunomodulatory effects. It synergistically alleviates heat stress in dairy cows through multiple targets, significantly improving issues such as elevated body temperature and decreased appetite caused by heat stress. It also enhances milk quality and yield, improves drug stability and palatability, making it more easily accepted by animals. This composition contains no chemical agents, has no toxic side effects, and will not leave harmful residues in the cow's body or milk. It combines prevention and early intervention, has a short processing route, low rumen burden, and meets export requirements for all testing items in one go. It better meets the actual needs of farms for powdered feed mixing, providing the livestock industry with an efficient, safe, and economical solution.

[0010] Xiangru Powder is a classic formula for clearing heat and relieving summer heat, composed of Xiangru (Elsholtzia ciliata), white hyacinth bean, and magnolia bark. It is mainly used to treat heatstroke and heat exhaustion in livestock and poultry, but its bitter taste makes it unpalatable. This invention addresses the characteristics of long course and high recurrence rate of heat stress in modern large-scale farming by modifying Xiangru Powder. It retains the core antipyretic ingredient Xiangru, removes the overlapping effects of magnolia bark and white hyacinth bean, and adds reed rhizome, trichosanthes root, and scrophularia root to promote body fluid production. Gardenia clears the stagnant fire in the triple burner, and coix seed (stir-fried with wheat bran) strengthens the spleen and eliminates dampness. Talc, tangerine peel, and licorice are retained but reduced in amount to maintain the dampness-eliminating and qi-regulating effects while improving palatability with a sweet, bland, and pungent aroma. The entire formula employs four parallel methods: "clearing summer heat, promoting diuresis, generating body fluids, and strengthening the spleen." This forms a dual pathway of lowering the central body temperature set point and increasing peripheral heat dissipation channels. As a result, summer heat is dispelled, body fluids are restored, and the spleen's function is strengthened, thus significantly improving the dry matter intake and milk production performance of dairy cows. However, the effects are significantly reduced when one or two herbs are replaced with herbs of similar efficacy (e.g., replacing dried tangerine peel with immature bitter orange peel) or when one or two herbs are missing (e.g., lacking scutellaria baicalensis).

[0011] In some specific embodiments, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: Talc 20 parts, Elsholtzia ciliata 20 parts, Scutellaria baicalensis 5 parts, Phragmites communis 10 parts, Scrophularia ningpoensis 10 parts, Trichosanthes kirilowii 10 parts, Gardenia jasminoides 10 parts, Coix lacryma-jobi 20 parts, Citrus reticulata 5 parts, and Glycyrrhiza uralensis 5 parts.

[0012] In some specific embodiments, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: Talc 30 parts, Elsholtzia ciliata 25 parts, Scutellaria baicalensis 8 parts, Phragmites communis 15 parts, Scrophularia ningpoensis 15 parts, Trichosanthes kirilowii 15 parts, Gardenia jasminoides 15 parts, Coix lacryma-jobi 25 parts, Citrus reticulata 10 parts, and Glycyrrhiza uralensis 15 parts.

[0013] In some specific embodiments, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: Talc 40 parts, Elsholtzia ciliata 30 parts, Scutellaria baicalensis 10 parts, Phragmites communis 20 parts, Scrophularia ningpoensis 20 parts, Trichosanthes kirilowii 20 parts, Gardenia jasminoides 20 parts, Coix lacryma-jobi 30 parts, Citrus reticulata 20 parts, and Glycyrrhiza uralensis 20 parts.

[0014] This invention provides a method for preparing the aforementioned traditional Chinese medicine composition, comprising: Stir-fry the Job's tears over low heat, let them cool, and then mix them with the remaining ingredients.

[0015] This invention uses roasted Job's tears in the formula. After roasting, the medicinal properties change from cool to neutral to slightly warm. The roasted aroma enters the spleen, promotes the secretion of digestive enzymes, and can also calm the mind. It is suitable for dairy cows with heat stress accompanied by spleen deficiency and dampness, decreased feed intake, diarrhea or indigestion.

[0016] In this invention, the temperature for stir-frying over a low flame is 110~130℃, specifically 110℃, 120℃, or 130℃. Cooling refers to cooling to room temperature. This invention does not impose special restrictions on the mixing method; it can be machine mixing or manual stirring. In a specific embodiment of this invention, a small household grinder is used for smaller quantities, and a low-temperature high-speed blender is used for larger quantities. The mixing specifically includes: pre-mixing talc, roasted Job's tears, and Elsholtzia ciliata, and then adding other raw materials for further mixing and pulverization. The total mixing time is greater than or equal to 15 minutes, and the specific time can be adjusted according to actual conditions. For example, when using a low-temperature high-speed blender with a loading capacity of 10L or more, approximately 30 minutes of vibration is required.

[0017] In this invention, the particle size of the traditional Chinese medicine composition is 80-100 mesh, meaning that the pulverized material can pass through an 80-100 mesh sieve. Specifically, the particle size can be 80, 90, or 100 mesh. Experimental verification shows that this particle size ensures the full dissolution of the active ingredients while preventing excessively fine particles from causing choking or rapid fermentation in the rumen during dairy cow consumption, thus affecting palatability and digestibility.

[0018] The present invention also provides the application of traditional Chinese medicine compositions in the preparation of drugs for relieving heat stress in animals.

[0019] The animal mentioned includes a rat or a cow. Further, the cow includes a dairy cow. In a specific embodiment of the invention, the animal is a cow, specifically a dairy cow.

[0020] In a specific embodiment of the present invention, the effect of the traditional Chinese medicine composition in relieving heat stress was verified using dairy cows as an example. Experiments showed that the traditional Chinese medicine composition of the present invention can not only significantly improve problems such as elevated body temperature and decreased appetite in dairy cows caused by heat stress, but also improve the quality and yield of dairy products.

[0021] The present invention also provides a medicine for relieving heat stress in dairy cows, comprising the traditional Chinese medicine composition as described above and acceptable excipients.

[0022] The traditional Chinese medicine composition of this invention may include commonly used excipients acceptable in the field of veterinary drug formulations, including but not limited to at least one of the following: diluents (such as pharmaceutical corn starch, anhydrous glucose, etc.), adsorbents (such as pharmaceutical silica), and dispersants (such as magnesium stearate). By selecting suitable excipients and following processes well known in the art, the traditional Chinese medicine composition of this invention can be formulated into common dosage forms, including but not limited to powders, granules, premixes, tablets, granules, capsules, pills, powders for injection, lyophilized powder for injection, tablets, granules, or liquids.

[0023] This invention provides a traditional Chinese medicine composition for alleviating heat stress in animals, its preparation method, and its application. The composition includes talc, *Elsholtzia ciliata*, *Scutellaria baicalensis*, *Phragmites communis* rhizome, *Scrophularia ningpoensis*, *Trichosanthes kirilowii*, *Gardenia jasminoides*, *Coix lacryma-jobi*, tangerine peel, and licorice. Based on the principles of traditional Chinese medicine compatibility, this invention rationally combines these 10 medicinal materials in a specific ratio. The resulting composition effectively alleviates heat stress in animals caused by high-temperature environments, significantly improves milk production in dairy cows, and enhances milk quality. In practical applications, it demonstrates good adaptability and is suitable for various farming scenarios, providing reliable protection for animal health. Attached Figure Description

[0024] Figure 1 The dehydration rate of mice after heat treatment; Figure 2 Changes in body temperature in mice after heat treatment; Figure 3 The performance score of mice after heat treatment (NC group with a score of 0 was not included in the analysis); Figure 4 Serum ALT levels in mice; Figure 5 This refers to the serum AST level in mice. Figure 6 The daily milk yield of the three groups of dairy cows in the experiment varied with the number of days of medication. Figure 7 To compare the average rumination time (A) and feeding time (B) of the three groups of dairy cows in the experiment; Figure 8 To analyze the stability of somatic cell number metabolic trajectory under heat stress; Figure 9 The daily milk yield of dairy cows in the two pens varied with the number of days of medication in the experiment; Figure 10 The variation in milk yield per cow in the two groups during the experiment with the number of days of medication; Figure 11 This study compares the milk yield per cow in the two groups during the early and later stages of the experiment. Figure 12 The variation of dry matter intake of dairy cows in the two pens over the number of days in the experiment; Figure 13This represents the dry matter intake of dairy cows in the two pens during the later stages of the experiment. Figure 14 The conversion efficiency of dairy cows in the two pens varied with the number of days in the experiment; Figure 15 The conversion efficiency of the two groups of dairy cows in the experiment varied with the number of days; Figure 16 The changes in conversion efficiency of the two groups of dairy cows before and after the experiment; Figure 17 The changes in milk composition in the two groups of dairy cows before and after the experiment are shown. A to C represent lactose content, milk protein content, and SCC, respectively. Detailed Implementation

[0025] This invention provides a traditional Chinese medicine composition for alleviating heat stress in animals, its preparation method, and its application. Those skilled in the art can refer to the content of this document and appropriately modify the process parameters to achieve the desired result. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention. The methods and applications of this invention have been described through preferred embodiments. Those skilled in the art can clearly modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit, and scope of this invention to realize and apply the technology of this invention.

[0026] The formula of this invention uses four methods in parallel: "clearing heat, promoting diuresis, generating body fluids, and strengthening the spleen". It can relieve external heat, protect body fluids, and prevent dampness from affecting the spleen and stomach. It is suitable for early prevention of heat stress in dairy cows during hot and humid seasons and as an adjunct to mild heatstroke.

[0027] Analysis of the formula's composition: Talc and Elsholtzia ciliata are the principal herbs, clearing heat, relieving summer heat, promoting diuresis, and alleviating irritability; Scutellaria baicalensis, Phragmites communis, Trichosanthes kirilowii, and Scrophularia ningpoensis are the assistant herbs, clearing heat, purging fire, generating fluids, and protecting yin; Coix lacryma-jobi (stir-fried with wheat bran), Gardenia jasminoides, and Citrus reticulata are the adjuvant herbs, strengthening the spleen, eliminating dampness, and promoting qi circulation; Glycyrrhiza uralensis is the guiding herb, harmonizing the effects of the other herbs to ensure proper dispersion and astringency. Together, these herbs achieve the effects of clearing summer heat, eliminating dampness, generating fluids, and strengthening the spleen.

[0028] The principal herbs are: Talc, which is sweet, bland, and cold, clears heat and relieves summer heat, drains dampness and promotes urination, allowing summer heat and dampness to be expelled through urine; and Elsholtzia ciliata, which is pungent, warm, and dispersing, relieves exterior symptoms and resolves dampness, helps summer heat to dissipate externally, and also invigorates the spleen. One cold and one warm herb are listed together as the principal herbs, guiding water and dampness to be expelled through urine and stagnant heat to be expelled through sweat. The combined effect of sweating and purging forms a dual pathway of "clearing the orifices and bladder," leaving no place for summer heat to remain, thus acting as the vanguard in opening the way.

[0029] The assistant herbs: Scutellaria baicalensis, bitter and cold, clears lung fire in the upper burner and assists the principal herb in dispelling summer heat; Phragmites communis and Trichosanthes kirilowii, sweet and cold, moisten and alleviate the bitterness and dryness of Elsholtzia ciliata and Scutellaria baicalensis, instantly saving stomach fluids; Scrophularia ningpoensis, salty and cold, cools the blood, nourishes fluids, detoxifies, and dissipates nodules. The four herbs work together to counteract the dryness of the principal herb and protect the depletion of yin fluids, so that the pathogenic factors are eliminated without harming the body's vital energy, specifically rescuing the danger of "summer heat inevitably damaging body fluids".

[0030] The adjuvant herbs are: stir-fried Job's tears, which invigorate the spleen, drain dampness, and stop diarrhea. Stir-frying reduces its cold nature and increases its warming effect on the spleen, helping to disperse talc and preventing the recurrence of dampness due to spleen deficiency; Gardenia clears heat from the triple burner and helps talc guide damp heat downwards; Tangerine peel regulates qi and dries dampness, so that when qi flows, dampness will also be eliminated, and it can also invigorate the spleen and stimulate appetite, preventing Job's tears from being too mild and hindering the stomach. With these three herbs working together, dampness has a way to go, heat has a way to descend, the spleen is strengthened, and the middle burner's pivotal function will naturally be restored.

[0031] The guiding herb, licorice, not only harmonizes the flavors but also, through its sweet and mild nature, moderates the cold and slippery properties of talc to protect the stomach. It also restrains the pungent and warm dispersing properties of eucommia to prevent excessive sweating that could harm the body's vital energy, thus achieving a balance between cold and warm properties and a proper distribution of dispersing and astringing effects, resulting in a harmonious overall formula.

[0032] The formula employs a balanced approach, balancing cold and heat. Talc relieves summer heat while Elsholtzia ciliata prevents dampness from accumulating, resulting in a balance of cold and heat that is "cool without being icy and warm without exacerbating the heat." It also promotes drainage and elimination, with Elsholtzia ciliata dispersing and talc descending to form a vertical drainage channel, while Coix seed and tangerine peel horizontally regulate the Qi of the middle Jiao, providing multiple pathways for the elimination of dampness and reducing overload on a single path. Furthermore, it simultaneously clears heat and generates fluids, with Scutellaria baicalensis and Gardenia jasminoides "extinguishing fire" while Reed rhizome, Trichosanthes kirilowii, and Scrophularia ningpoensis "replenish water," avoiding the need for clearing before replenishing or replenishing in a way that lingers on the pathogenic factors, leading to a stalemate between pathogenic and healthy Qi. The combination of Coix seed draining dampness and tangerine peel regulating Qi prevents the vicious cycle of "dampness stagnation → Qi stagnation → further dampness stagnation," and the aromatic tangerine peel invigorates the spleen, reducing the bloating sensation caused by the mild and sweet nature of Coix seed. Finally, the buffering effect of licorice ensures that the entire formula is "not excessive in inducing sweating and not icy in clearing heat."

[0033] Overall, this formula disperses external pathogens without being harsh, clears internal damp-heat without being cold, generates fluids without being greasy, and strengthens the spleen without causing stagnation. When used for dairy cows suffering from summer heat and dampness affecting the spleen, it can reduce fever, quench thirst, clear urine, solidify feces, and restore normal appetite. When used in the early stages of heat stress, it can also prevent problems before they occur, maintain fertility, and increase milk production.

[0034] The traditional Chinese medicine composition provided by this invention adopts an adaptive feeding method with gradually increasing dosage, so that dairy cows can gradually get used to it, while taking into account both prevention and early intervention, and can be fed in advance before the arrival of summer heat.

[0035] The preparation method of the traditional Chinese medicine composition of this invention has a short process route, low rumen burden, and the test items meet export requirements in one go, which is more in line with the actual needs of pasture powder mixed feed.

[0036] In this document, the terms "including", "comprising", and "having" describe both closed-loop technical solutions consisting of the listed features and open-loop technical solutions that include the listed features.

[0037] In this document, numerical ranges are referred to as continuous unless otherwise specified, and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when a range refers to an integer, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe a feature or characteristic, the ranges may be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are incorporated.

[0038] In this article, when referring to units of data ranges, if the unit is only followed by the right endpoint, it means that the units of the left and right endpoints are the same. For example, "20-40 parts" means that the units of the left endpoint "20" and the right endpoint "40" are both "parts".

[0039] This document only specifically discloses some numerical ranges. However, any lower limit can be combined with any upper limit to form an unspecified range; and any lower limit can be combined with other lower limits to form an unspecified range, just as any upper limit can be combined with any other upper limit to form an unspecified range. Furthermore, each individually disclosed point or single value can itself serve as a lower or upper limit and be combined with any other point or single value or with other lower or upper limits to form an unspecified range.

[0040] The test materials used in this invention are all common commercial products and can be purchased on the market.

[0041] The present invention will be further illustrated below with reference to the embodiments:

[0042] Example 1 Formula: 40 parts talc, 30 parts euphorbia humifusa, 10 parts scutellaria baicalensis, 20 parts reed rhizome, 20 parts scrophularia ningpoensis, 20 parts trichosanthes kirilowii, 20 parts gardenia, 30 parts coix seed, 20 parts tangerine peel, and 20 parts licorice.

[0043] Preparation method: (1) Raw material pretreatment: Clean and select, ensure that all medicinal materials are fully dried, and store at room temperature.

[0044] (2) Coarse crushing: each of the following herbs, including Elsholtzia ciliata, Phragmites communis, Scrophularia ningpoensis, Trichosanthes kirilowii, Gardenia jasminoides, Scutellaria baicalensis, Citrus reticulata peel, and Glycyrrhiza uralensis, should be coarsely crushed and passed through a No. 2 sieve beforehand.

[0045] (3) Mixing: According to the prescription ratio, first put the large quantities of talc, stir-fried coix seed, and eucommia into a pulverizer (fixed speed n=50 r / s) and run it for premixing for 5 minutes; then add all the remaining medicinal materials in the order of "heavy first, light later; roughly first, fine later" (trichosanthes root, reed rhizome, scrophularia root, gardenia fruit, scutellaria root, licorice root, tangerine peel), and run it intermittently for about 20 minutes to perform deep cell wall breaking and pulverization. The total time can be adjusted appropriately to about 30 minutes according to the characteristics of the materials.

[0046] (4) Sieve the mixed powder through an 80-mesh vibrating screen. Unsieved fibers are crushed again and added back in. Coarse fibers that are difficult to crush can be discarded as ineffective impurities.

[0047] (5) Pack in separate containers and store in airtight, moisture-proof and light-proof containers.

[0048] Example 2

[0049] Formula: 20 parts talc, 20 parts euphorbia humifusa, 5 parts scutellaria baicalensis, 10 parts reed rhizome, 10 parts scrophularia ningpoensis, 10 parts trichosanthes kirilowii, 10 parts gardenia, 20 parts coix seed, 5 parts tangerine peel, and 5 parts licorice.

[0050] Preparation method: (1) Raw material pretreatment ① Stir-fried Job's tears: Take clean Job's tears and stir-fry them over a low heat at 110-130℃ until the surface is slightly yellow, the crack rate is 5-10%, and they emit the aroma of roasted grains without any burnt smell. Cool to room temperature. ② Other medicinal materials: clean and select, and store at room temperature.

[0051] (2) Coarsely crushed: each of the following herbs is coarsely crushed into relatively uniform small segments or granules (2-3 cm³): Mosla, Phragmites communis, Scrophularia ningpoensis, Trichosanthes kirilowii, Gardenia jasminoides, Scutellaria baicalensis, Citrus reticulata peel, and Glycyrrhiza uralensis.

[0052] (3) Mixing: According to the prescription ratio, first put the large quantities of talc, stir-fried coix seed, and eucommia into a low-temperature wall-breaking machine and run it at a low amplitude for 5-10 minutes for premixing; then add all the remaining medicinal materials in the order of "heavy first, light later; roughly first, fine later" (trichosanthes root, reed rhizome, scrophularia root, gardenia fruit, scutellaria root, licorice root, tangerine peel), and adjust to a medium-high amplitude for about 20 minutes for deep wall-breaking and pulverization. The total time can be adjusted to about 30 minutes according to the characteristics of the materials. Among them, 4.5-5.0mm (medium of medium-high amplitude) is used for rapid wall breaking, and 2.5-3.0mm (medium of medium-low amplitude) is used for ultra-fine pulverization.

[0053] (4) Sieve the mixed powder through an 80-mesh vibrating screen. Unsieved fibers are crushed again and added back in. Coarse fibers that are difficult to crush can be discarded as ineffective impurities.

[0054] (5) Pack in separate containers and store in airtight, moisture-proof and light-proof containers.

[0055] Example 3

[0056] Formula: 30 parts talc, 25 parts elsholtzia ciliata, 8 parts scutellaria baicalensis, 15 parts reed rhizome, 15 parts scrophularia ningpoensis, 15 parts trichosanthes kirilowii, 15 parts gardenia, 25 parts coix seed, 10 parts tangerine peel, and 15 parts licorice.

[0057] The preparation method is the same as in Example 2.

[0058] Comparative Example 1 (lacking Scrophularia and Tianfang powder compared to Example 1)

[0059] Formula: 40 parts talc, 30 parts euphorbia humifusa, 10 parts scutellaria baicalensis, 20 parts reed rhizome, 20 parts gardenia, 30 parts coix seed, 20 parts tangerine peel, and 20 parts licorice.

[0060] The preparation method is the same as in Example 1.

[0061] Comparative Example 2 (lacking Scutellaria baicalensis and Coix lacryma-jobi compared to Example 1)

[0062] Formula: 40 parts talc, 30 parts euphorbia humifusa, 20 parts reed rhizome, 20 parts scrophularia root, 20 parts trichosanthes root, 20 parts gardenia, 20 parts dried tangerine peel, and 20 parts licorice root.

[0063] The preparation method is the same as in Example 1.

[0064] Comparative Example 3 (Substitution of Example 1: Gardenia is replaced with Anemarrhena asphodeloides, and Citrus reticulata peel is replaced with Citrus aurantium)

[0065] Formula: Talc 40 parts, Elsholtzia ciliata 30 parts, Scutellaria baicalensis 10 parts, Phragmites communis 20 parts, Scrophularia ningpoensis 20 parts, Trichosanthes kirilowii 20 parts, Anemarrhena asphodeloides 20 parts, Coix lacryma-jobi 30 parts, Citrus aurantium 20 parts, Glycyrrhiza uralensis 20 parts.

[0066] The preparation method is the same as in Example 1.

[0067] Comparative Example 4 (lacking Scutellaria baicalensis compared to Example 2)

[0068] Formula: 20 parts talc, 20 parts euphorbia humifusa, 10 parts reed rhizome, 10 parts scrophularia root, 10 parts trichosanthes root, 10 parts gardenia, 20 parts coix seed, 5 parts dried tangerine peel, and 5 parts licorice.

[0069] The preparation method is the same as in Example 2.

[0070] Test Example 1: Mouse Feeding Experiment

[0071] (a) Experimental Design

[0072] Seventy-two ICR mice (SPF grade, 6-8 week old females) were randomly divided into seven groups: a model group of 12 mice and other groups of 10 mice each. After acclimatization, the mice in each heat-treated group were placed in a constant temperature and humidity chamber (39℃, 60%RH) for 2 h / d, while the mice in the blank control group were placed under normal temperature and humidity conditions (20℃, 40%RH) (see Table 1). The treatment was carried out for 20 consecutive days. The mice's body weight, food intake, body temperature changes before and after heat treatment, and behavioral performance were recorded daily. At the final treatment, blood was collected, organs were weighed, and biochemical indicators were detected. The treatment groups were fed the same weight of feed containing the same amount of raw Chinese medicine composition, i.e., each mouse was given 2.5 mg of raw medicine / day.

[0073] The grouping is shown in the table below: Table 1. Grouping of mice in the experiment

[0074] (II) Experimental Results and Analysis

[0075] 1. Calculate the dehydration status of each group after heat treatment [( 前 - 后 ) / 前 [×100%], the result is as follows Figure 1 As shown, the dehydration in each group was significantly more severe than that in the NC group (P < 0.01), while the dehydration in TCM3 was significantly less severe than that in the HS group (P < 0.05), indicating that formulation 3 had a significant alleviating effect on the dehydration caused by heat treatment. The efficacy ranking of the compositions in each example was: TCM1 (Example 1) > TCM4 (Comparative Example 3) > TCM3 (Comparative Example 2) > TCM2 (Comparative Example 1) > Vitamin C.

[0076] 2. Calculate the change in body temperature of mice before and after heat treatment in each group: ΔT = T 后 -T 前； The result is as follows Figure 2 As shown, the HS group was significantly higher than the other groups, the NC group was significantly lower than the other groups, and TCM1 (Example 1) was significantly lower than TCM2 (Comparative Example 1). The significance is as follows: Figure 2 As shown (P < 0.05). The efficacy ranking of the compositions in each example is as follows: TCM1 (Example 1) > TCM4 (Comparative Example 3) > TCM3 (Comparative Example 2) > Vitamin C > TCM2 (Comparative Example 1).

[0077] 3. A rating standard was established based on the behavioral and other performance of mice after heat treatment, as shown in Table 2. Mice were scored after each heat treatment, and the results are as follows: Figure 3 As shown, the HS group scored significantly higher than the other groups, the PC group significantly outperformed TCM2 (Comparative Example 1) and TCM3 (Comparative Example 2), and TCM1 (Example 1) significantly outperformed TCM2 (Comparative Example 1) (P < 0.05). The efficacy ranking of the compositions in each example was: Vitamin C > TCM1 (Example 1) > TCM4 (Comparative Example 3) > TCM3 (Comparative Example 2) > TCM2 (Comparative Example 1).

[0078] Table 2 Behavioral Scoring Table for Heat Stress in Mice

[0079] 4. Before final processing, collect mouse blood, centrifuge to obtain the supernatant, and detect the AST and ALT levels as follows: Figure 4 , Figure 5 As shown, the AST value of mice in the HS group was significantly higher than that of all other groups (P < 0.05), and the ALT value was extremely significantly higher than that of all other groups (P < 0.01). Among them, the AST value of TCM1 (Example 1) was significantly lower than that of the PC group (P < 0.05). This suggests that all drugs have the effect of relieving heat stress, and formulation 4 may have a super-repair effect on AST. The efficacy ranking of the compositions in each example is: TCM1 (Example 1) > TCM4 (Comparative Example 3) > TCM3 (Comparative Example 2) > TCM2 (Comparative Example 1) > Vitamin C.

[0080] 5. Based on the above experimental data, the compositions in each embodiment showed clear therapeutic or alleviating effects on the heat stress mouse model. The comprehensive technical indicators are evaluated as follows: Example 1 (TCM1) showed the best performance among multiple core indicators: its dehydration control effect was significantly better than that of Comparative Example 1 (P < 0.05), its thermoregulation ability was the strongest (ΔT value was significantly lower than that of Comparative Example 1), and its liver function protection effect was outstanding (AST value was significantly lower than that of the positive control group, P < 0.05), ranking first in overall efficacy. In other words, the composition of this invention has a comprehensive and significant protective effect against heat stress, especially in thermoregulation and liver damage repair.

[0081] Comparative Example 3 (TCM4, the replacement for Example 1) showed stable and reliable overall performance. It was second only to Example 1 in terms of dehydration inhibition, body temperature regulation, and improvement of AST / ALT indicators, ranking second in the overall ranking. It also showed a possible super-repair effect on AST, demonstrating good dose-response relationship characteristics.

[0082] Comparative Example 2 (TCM3, the missing formula of Example 1 - lacking Scutellaria baicalensis and Coix lacryma-jobi) was significantly better than the simple heat stress group (HS group) in terms of dehydration control (P < 0.05), and other indicators were all at a moderate to high level. It ranked third in terms of overall efficacy and had a clear anti-heat stress effect.

[0083] Comparative Example 1 (TCM2, the missing formula of Example 1 - lacking Scrophularia and Tianfang powder) was relatively weaker in terms of thermoregulation and behavioral improvement, but it was still significantly better than the HS group in terms of dehydration inhibition and liver function protection, and the overall effect was better than vitamin C alone.

[0084] Overall conclusion: All the traditional Chinese medicine compositions in the examples effectively alleviated dehydration, elevated body temperature, liver function damage, and behavioral abnormalities caused by heat stress. Example 1 (TCM1) showed the most comprehensive and significant technical effects and is the optimal implementation scheme; Comparative Examples 3 (TCM4) and 2 (TCM3) are preferred schemes. The compositions of this invention are generally superior to conventional vitamin C controls in their anti-heat stress effects and possess industrial production value.

[0085] Test Example 2: Dairy Cow Feeding Trial

[0086] (I) Experimental Design

[0087] Thirty Holstein dairy cows with similar body condition, weight, age, and milk yield, and ≥30 days remaining in their dry period, were randomly selected. Based on parity, number of days in lactation, and gestation status, they were stratified and divided into three experimental groups: a blank control group (C) and a traditional Chinese medicine group. The traditional Chinese medicine group included two subgroups: group A (fed with traditional Chinese medicine) and group B (fed with traditional Chinese medicine), with 10 cows in each group. Group B, fed with traditional Chinese medicine: fed the traditional Chinese medicine composition of Example 2.

[0088] Group A, fed with traditional Chinese medicine: fed the traditional Chinese medicine composition of Comparative Example 4 (which lacked Scutellaria baicalensis compared to the traditional Chinese medicine composition of Example 2, i.e., the traditional Chinese medicine composition of Group A did not contain Scutellaria baicalensis).

[0089] The control group was fed a normal basal diet without any added traditional Chinese medicine (TCM) herbs. The TCM herbal group received a TCM herbal composition at a rate of 50g / day / head, added manually and evenly to the TMR diet after feeding at 9:30 AM daily for 14 consecutive days. During the experiment, cows had free access to feed and water, and were allowed free-range exercise. Infrared thermometers were used to measure the cows' body temperature at 2:00 PM daily, and rumination duration was monitored using the farm's neck ring system. Milk samples were collected from the first milking shift on days 0 and 14 (four udders) and tested on the same day.

[0090] (II) Experimental Results and Analysis

[0091] As shown in Table 3, during three consecutive days of high temperatures (Note: 0d in this table refers to the day before the arrival of a high-temperature day; during the adaptive feeding phase, the average body surface temperature of group A was 32.43±0.60°C, lower than that of group B, which was 32.50±0.52°C), the body surface temperature of the control group remained high. The traditional Chinese medicine groups A (containing the formula lacking Scutellaria baicalensis) and B (containing the complete formula of this invention) were significantly lower than the control group, and group B was slightly better (according to the analysis of variance, group B showed a significant difference earlier than the control group C, indicating that it took effect faster), indicating that adding the traditional Chinese medicine composition of this invention can alleviate heat stress in dairy cows.

[0092] Table 3. Changes in body surface temperature of dairy cows during consecutive days of high temperatures (°C) in the experiment.

[0093] Note: Different lowercase letters indicate significant differences between groups (p < 0.05).

[0094] Depend on Figure 6 It can be seen that the compound Chinese medicine B significantly mitigated the decline in daily milk production caused by heat stress, while the blank and Chinese medicine A both showed a faster decline trend, proving that the compound Chinese medicine of the present invention effectively overcomes the negative impact of heat stress on milk production.

[0095] Depend on Figure 7It can be seen that the feeding time of both herbal medicine groups was longer than that of the blank control group. Among them, the dairy cows taking herbal medicine compound B had the shortest rumination time and the longest feeding time, and both were statistically significantly different from the blank control group. Combined with the above analysis of body temperature and milk production data, it is suggested that this formula may redistribute some of the energy used for rumination and heat production to heat dissipation and production through an energy optimization strategy. From the perspective of overall production efficiency and animal welfare, the herbal medicine compound of this invention effectively and economically overcomes the negative impact of heat stress on milk production.

[0096] Under heat stress, the energy metabolism balance of dairy cows is easily disrupted, and one of the key indicators is the fat-to-protein ratio (FPR). Since most of the experimental cows in this batch were in the mid-to-late lactation stage (lactation days > 200 days), their mammary glands' ability to synthesize milk fat was weakened, resulting in a physiological decrease in milk fat percentage, while milk protein percentage remained relatively stable, leading to an overall low average FPR for the herd. Table 2-2 shows that in this study, the FPR of the blank control group C unexpectedly increased during the experimental period. This may reveal the natural trend of accelerated decline in milk protein synthesis function or decreased metabolic coordination and abnormal mobilization patterns in dairy cows under uninterrupted conditions during the mid-to-late lactation stage. In stark contrast, both groups fed with traditional Chinese medicine (TCM) experienced a decrease in FPR, which stabilized at an even lower level. We speculate that this reflects the multi-target regulatory effect of TCM: maintaining mammary gland health and supporting stable milk protein synthesis while guiding the body's energy metabolism towards a more efficient utilization mode, thereby restoring the production of milk fat and milk protein to a more coordinated and stable new balance. This result suggests that the main effects of traditional Chinese medicine compositions A and B in the late lactation stage may be to delay the decline in dairy cow productivity and improve the quality of the decline (manifested as a more harmonious milk composition), rather than simply changing the absolute value of a certain indicator.

[0097] Since mean changes cannot reflect the dispersion and dynamic stability of metabolic states within a cattle herd, this invention constructs a multi-dimensional metabolic trajectory stability analysis model based on dynamic data of milk fat-protein ratio (FPR). This model not only focuses on the changing trend of the mean FPR, but also innovatively introduces changes in intragroup dispersion and metabolic entropy change (ΔS) as core evaluation dimensions.

[0098] Maintenance rate: characterizes the retention level of the mean FPR at the end of the experiment (D14) relative to the beginning of the experiment (D0). The calculation formula is: Maintenance rate (%) = (mean D14 / mean D0) × 100%. A maintenance rate close to 100% indicates that the overall metabolic level tends to stabilize.

[0099] Rate of change of standard deviation: quantifies the change in the dispersion of individual FPR values ​​within a group during the trial period. The formula is: Rate of change of standard deviation (%) = [(D14 standard deviation - D0 standard deviation) / D0 standard deviation] × 100%. A negative value indicates that individual differences within the group have narrowed and responses have converged.

[0100] Metabolic entropy change (ΔS): A key indicator defined in this invention, used to measure the orderly change of metabolic state from an information theory perspective. Its calculation formula is: ΔS = ln(SD1 / SD0), where SD0 is the standard deviation of FPR at D0, SD1 is the standard deviation of FPR at D14, and ln is the natural logarithm.

[0101] ΔS < 0: This indicates that after intervention, the orderliness of the population's metabolic state increases, the dispersion decreases, and the metabolic trajectory shows a trend of "convergence" and "stabilization," corresponding to positive and high-quality metabolic regulation.

[0102] ΔS > 0: This indicates an increase in the disorder and dispersion of the population's metabolic state, with the metabolic trajectory showing a trend of "divergence" and "instability," corresponding to negative metabolic disorders or decompensation.

[0103] As shown in Table 4, although the changes in FPR in the three groups were not statistically significant, the traditional Chinese medicine group B had the smallest mean change and extremely high maintenance rate, and its ΔS was negative and the absolute value of the SD change rate was the smallest, revealing its irreplaceable advantages. The formula-deficient group A also showed some effect; although there was entropy reduction, the decrease was too large, indicating that the formula lacked preventative regulatory ability. The blank control group C, on the other hand, exhibited typical metabolic instability and dispersion in dairy cows after heat stress injury.

[0104] Table 4. Stability analysis of milk lipoprotein ratio FPR metabolic trajectory under heat stress

[0105] Table 5 shows that the metabolic entropy change (ΔS≈0) of lactose rate in the blank control group exhibited high mathematical stability, but it remained at the industry critical level of 4.55% for a long period, revealing that under no intervention conditions, the mammary gland function of dairy cows in late lactation is prone to slide into and "rigidify" into a low-functional compensatory state. Conversely, although the traditional Chinese medicine group B was accompanied by a slight entropy increase (ΔS=+0.120), its lactose rate successfully improved and stabilized at an excellent level of 4.79%. This limited dispersion leading to better results at a high healthy baseline precisely reflects the positive and differentiated positive response of the herd to the traditional Chinese medicine intervention, which is a manifestation of good metabolic activity and functional reserves, consistent with the characteristics of "robust regulation". While the lactose rate of the traditional Chinese medicine group A decreased slightly, its dispersion also decreased, achieving "high-level convergence", which is also a valuable regulatory model, but slightly inferior to that of group B.

[0106] Table 5 Stability analysis of lactose rate metabolic trajectory under heat stress

[0107] As shown in Table 6 and Figure 8 As shown, the somatic cell count in the blank control group (C) increased sharply during the experimental period (16.25→23.28), accompanied by significant individual variability (ΔS=+0.381, SE=10.116). This conclusively demonstrates that under no-intervention conditions, there is a clear risk of deterioration and uncontrolled management uncertainty in the mammary gland health of dairy cows. Both traditional Chinese medicine compositions (A and B) successfully blocked this deteriorating trend. Among them, the group without the prescription A showed a "therapeutic" response, with a decrease in somatic cell count and a tendency towards a more uniform population response (ΔS=-0.421). However, the herbal feeding regimen B of this invention exhibits a superior "steady-state management" response: Group B stabilized the somatic cell count at an excellent level of 12.10, lower than Group A's 13.43. In animal husbandry, achieving and maintaining a lower health risk threshold is of decisive significance. The larger effect size (g=0.8 > 0.7) and smaller standard error (SE=1.113 <2.126) mean that Group B's improvement effect is not only greater in magnitude but also more reproducible and predictable. At the same time, the metabolic entropy change of Group B (ΔS≈0) indicates that it has higher population stability and certainty in the experiment.

[0108] Table 6. Stability analysis of somatic cell number metabolic trajectory under heat stress

[0109] In summary, as illustrated in the charts and discussion above, the herbal formula B (the complete formula) of this invention does not pursue excellence in a single indicator. Instead, through multi-target and systemic regulation, it synergistically promotes the transformation of dairy cows towards a "high-yield, high-efficiency, and high-health" steady state under heat stress in four key areas: body temperature control, production maintenance, metabolic balance, and mammary gland health. Its "prevention-stabilization-optimization" mode of action, compared to the "correction-treatment" mode of Group A, represents a paradigm shift from "managing existing problems" to "creating a resilient and stable production system." Therefore, it possesses irreplaceable innovative value and application prospects in alleviating heat stress in dairy cows and improving overall production efficiency and animal welfare.

[0110] Test Example 3: TMR Feeding Experiment in Dairy Cows

[0111] (a) Experimental Design

[0112] Holstein dairy cows with similar body condition, weight, age, and milk production, and with at least 30 days remaining before the dry period, were selected and stratified into a compound experimental group and a blank control group (22 cows in each group) based on parity, number of days in lactation, and pregnancy status. The compound experimental group was administered the drug (the herbal composition from Example 3) by mixing it into their TMR diet at 9:30 AM daily, with the dosage gradually increasing to 50g of raw herb / day / cow (within one week), for 40 consecutive days. During the experiment, cows had free access to feed and water and were allowed free-range exercise.

[0113] After milking the cows during the second shift each day, rectal temperature was measured using an M900 electronic thermometer. Respiratory rate, milk yield, and feed intake were also recorded. Milk samples (from all four udders) were collected during the first milking shift on days D0 and D30 and tested on the same day.

[0114] In this embodiment, the drug administration was conducted using the barn as the observation unit. Twenty-two cows were selected from two barns with similar initial conditions and divided into a blank control group and a compound experimental group for priority observation. The conversion efficiency data obtained from both observation units indicated that the experimental group (experimental barn) was significantly better than the control group (control barn), verifying the drug's effectiveness. Specific results are shown in the experimental results and analysis below.

[0115] (II) Experimental Results and Analysis

[0116] Table 7 shows that the rectal temperature of dairy cows in the blank control group remained consistently high, and increased with the number of experimental days. The rectal temperature of dairy cows fed the compound formula was initially higher than that of the blank control group, then decreased with the number of experimental days, becoming lower than the control group by day 7, and significantly lower by day 15, subsequently remaining consistently lower and statistically significant. These results indicate that the traditional Chinese medicine compound of this invention helps dairy cows relieve exterior heat and regulate core body temperature, significantly alleviating heat stress symptoms.

[0117] Table 7. Changes in rectal temperature of dairy cows in the two groups during the experiment (°C) as the number of days of medication (0-40 days) increased.

[0118] Note: This indicates a significant difference between groups (p < 0.05). This indicates that the difference between groups is extremely significant (p < 0.01).

[0119] Table 8 shows that the respiratory rate of dairy cows fed the compound was initially higher than that of the control group, gradually decreased to lower than that of the control group as the number of experimental days increased, stabilized at a lower level after 10 days, and remained significantly lower after 25 days. The results indicate that continuous administration of the herbal compound of this invention can significantly alleviate the respiratory tachypnea symptoms in dairy cows caused by heat stress.

[0120] Table 8. Changes in respiratory rate (breaths / min) of dairy cows in the two groups during the experiment.

[0121] Note: This indicates a significant difference between groups (p < 0.05).

[0122] Table 9. Correlation analysis between rectal temperature and THI in two groups of dairy cows

[0123] Note: The results showed that rectal temperature in the blank control group was highly significantly correlated with THI (p < 0.01).

[0124] Table 10 Correlation analysis between respiratory rate and THI (temperature and humidity index) in two groups of dairy cows

[0125] Note: The results indicate that the respiratory rate in the compound test group was significantly correlated with THI (p < 0.01). This indicates that respiratory rate in the blank control group was highly significantly correlated with THI (p < 0.001).

[0126] As shown in Tables 9-10, the correlation between rectal temperature, respiratory rate, and THI index in the blank control group (r=0.604, p=0.006; r=0.807, p<0.001) was stronger than that in the compound experimental group (r=0.442, p=0.058; r=0.690, p=0.009), suggesting that the physiological indicators of the blank control group were more easily driven by the environment. Fisher's Z test showed that although the differences in the correlation coefficients of rectal temperature and respiratory rate between the control group and the experimental group were not statistically significant (Z1=0.637, Z2=0.604, p>0.05), the correlation coefficients of the experimental group decreased by 26.8% (rectal temperature) and 14.5% (respiratory rate), respectively. This suggests that the herbal composition of the present invention can weaken the interference of heat stress on core temperature and respiratory rate, that is, the drug may help dairy cows maintain physiological homeostasis by participating in the regulation of environmental stress. (Note: The above correlation analysis is a mechanism exploration study. The technical effect of this invention is based on the significant improvement of specific physiological indicators in Tables 7 and 8.)

[0127] Depend on Figure 9 It can be seen that the overall milk yield of the blank control pen was higher than that of the compound experimental pen. In the early stage of the experiment, the milk yield per cow in both pens showed a downward trend. However, as the compound TMR was mixed into the feed, the milk yield per cow in the experimental pen showed an upward trend, while the milk yield per cow in the control pen continued to decline due to the arrival of summer. The difference in trends between the two pens was extremely significant. That is, the compound TMR of the present invention effectively overcomes the impact of heat stress on milk production.

[0128] Depend on Figure 10 , Figure 11 As shown, at the beginning of the experiment, the milk production level in the compound treatment group was slightly lower than that in the control group. As the drug's effects accumulated, the difference between the two groups gradually narrowed, and the experimental group eventually surpassed the control group. A t-test was performed on the milk production of the two groups during the first 10 days and the last 10 days of the experiment. The results showed that the milk production level in the control group decreased significantly (P < 0.001), while the milk production level in the compound treatment group increased significantly (P < 0.01). In summary, the milk production data indicate that continuous administration of the compound treatment reversed the heat stress-induced milk production decline in the dairy cows, maintained milk production stability under high temperature and humidity conditions, and is beneficial to improving the economic benefits of the dairy farm.

[0129] Table 11. Dry matter intake and its coefficient of variation of dairy cows in the two pens throughout the entire experimental period.

[0130] From Table 11 and Figure 12 , Figure 13 (In the later stages of the experiment) it was found that although the mean dry matter intake of dairy cows in the control pens was higher, its fluctuation was also greater. In the early stages of the experiment, the dry matter intake in the compound test pens was lower and more diffusely distributed compared to the blank control pens. However, as the drug effect accumulated, the dry matter intake in the test pens stabilized at a certain level and was significantly higher than that in the control pens in the later stages of the experiment (P < 0.05), while the blank control pens showed a significant downward trend. Linear analysis showed that the downward trend in the control pens was significantly greater than that in the test pens, indicating that the compound alleviated the impact of high temperature stress on dairy cow feed intake and dry matter intake.

[0131] Depend on Figure 14 , Figure 15 , Figure 16 In both the compound experimental group and the compound experimental pen, the conversion efficiency showed an upward trend during the experiment, which was significantly different from the downward trend in the blank control group. Furthermore, the conversion efficiency of the experimental group was lower in the early stage of the experiment, but significantly higher than that of the control group in the later stage. This indicates that the compound of this invention can effectively promote the improvement of milk conversion efficiency after dairy cows ingest dry matter. Combined with the fact that the body temperature of the experimental group was relatively lower than that of the control group, this suggests that the traditional Chinese medicine compound of this invention effectively relieves exterior symptoms, reduces heat dissipation and energy consumption in dairy cows, and directs energy towards lactation.

[0132] Depend on Figure 17 It can be seen that, affected by the increase in lactation period and the high temperature and humidity environment, the lactose rate and milk protein rate of both groups decreased, but the decrease was greater in the blank control group, significantly lower than the level before the start of the compound experimental group; the somatic cell count (SCC) of the cows in the blank control group increased significantly during the experiment, while that in the compound experimental group decreased under the regulation of the traditional Chinese medicine compound. That is, the traditional Chinese medicine compound of this invention helps to adjust the milk composition and improve the milk quality of heat-stressed dairy cows.

[0133] in conclusion: The results above show that the rectal temperature and respiratory rate of dairy cows fed the traditional Chinese medicine compound for alleviating heat stress in dairy cows according to this invention decreased, indicating that the various physiological indicators of cows under heat stress gradually returned to normal. At the beginning of the experiment, the milk production of the experimental group and the blank control group were similar. However, as the experiment progressed, the milk production of the experimental group was significantly higher than that of the control group, and the dry matter intake and conversion efficiency were also significantly higher. These results demonstrate that the traditional Chinese medicine composition provided by this invention can effectively alleviate the harm of heat stress to dairy cows and can improve milk production and milk quality.

[0134] The above are merely preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition, characterized in that, The ingredients include the following parts by weight: Talc 20-40 parts, Elsholtzia ciliata 20-30 parts, Scutellaria baicalensis 5-10 parts, Phragmites communis 10-20 parts, Scrophularia ningpoensis 10-20 parts, Trichosanthes kirilowii 10-20 parts, Gardenia jasminoides 10-20 parts, Coix lacryma-jobi 20-30 parts, Citrus reticulata 5-20 parts, and Glycyrrhiza uralensis 5-20 parts.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, Made from the following parts by weight of raw materials: Talc 20 parts, Elsholtzia ciliata 20 parts, Scutellaria baicalensis 5 parts, Phragmites communis 10 parts, Scrophularia ningpoensis 10 parts, Trichosanthes kirilowii 10 parts, Gardenia jasminoides 10 parts, Coix lacryma-jobi 20 parts, Citrus reticulata 5 parts, and Glycyrrhiza uralensis 5 parts.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, Made from the following parts by weight of raw materials: Talc 30 parts, Elsholtzia ciliata 25 parts, Scutellaria baicalensis 8 parts, Phragmites communis 15 parts, Scrophularia ningpoensis 15 parts, Trichosanthes kirilowii 15 parts, Gardenia jasminoides 15 parts, Coix lacryma-jobi 25 parts, Citrus reticulata 10 parts, and Glycyrrhiza uralensis 15 parts.

4. The traditional Chinese medicine composition according to claim 1, characterized in that, Made from the following parts by weight of raw materials: Talc 40 parts, Elsholtzia ciliata 30 parts, Scutellaria baicalensis 10 parts, Phragmites communis 20 parts, Scrophularia ningpoensis 20 parts, Trichosanthes kirilowii 20 parts, Gardenia jasminoides 20 parts, Coix lacryma-jobi 30 parts, Citrus reticulata 20 parts, and Glycyrrhiza uralensis 20 parts.

5. The method for preparing the traditional Chinese medicine composition according to any one of claims 1 to 4, characterized in that, include: Stir-fry the Job's tears over low heat, let them cool, and then mix them with the remaining ingredients.

6. The preparation method according to claim 5, characterized in that, The mesh size of the traditional Chinese medicine composition is 80-100 mesh.

7. The use of the traditional Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a drug for relieving heat stress in animals.

8. The application according to claim 7, characterized in that, The animals mentioned include rats or cattle.

9. The application according to claim 8, characterized in that, The cattle mentioned include dairy cows.

10. A drug for relieving heat stress in animals, characterized in that, It includes the traditional Chinese medicine composition according to any one of claims 1 to 4 and acceptable excipients.

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