Sample cutting mold and sample sampling method thereof

Abstract

This application relates to the field of sample cutting technology, and discloses a sample cutting mold and its sample sampling method. The sample cutting mold includes a cutting template; a plurality of cutting blades that are in close contact with each other are arranged on the cutting template; each cutting blade includes a blade body; the blade body is a three-dimensional polygonal prism, and the inner side of the blade body has a through hole; the first end of the blade body serves as a cutting edge, which is polygonal; the second end of the blade body serves as a sampling port; the cutting edge and the sampling port are arranged opposite to each other. Using the sample cutting mold provided in this application not only ensures the integrity of biological cell samples as much as possible, but also avoids sample loss, reducing costs compared to high-cost laser micro-cutting equipment. Furthermore, the sample cutting mold provided in this application is compatible with automation, reducing manual operation and improving experimental efficiency and repeatability.

Description

Technical Field

[0001] This application relates to the field of sample cutting, and in particular to a sample cutting mold and a sample sampling method thereof. Background Technology

[0002] In the biomedical field, spatial proteomics has become an important tool for studying complex molecular interactions within organisms. The core objective of spatial proteomics is to reveal the distribution and interactions of proteins in three-dimensional space, particularly their in situ expression and localization in cells or tissue sections. Through spatial proteomics, we can gain a deeper understanding of biological processes such as signal transduction, energy metabolism, and gene expression differences among neighboring tissues or cells. This provides a novel perspective for research on cancer development and progression, the analysis of the tumor microenvironment, and the distribution of abnormal proteins in neurodegenerative diseases.

[0003] Despite significant advancements in space omics technologies, current biological sample sampling methods still face limitations, particularly in balancing high resolution and high throughput. Achieving sufficient spatial resolution requires extremely high precision in the sampling process, while simultaneously preserving as much cell or tissue integrity as possible. However, existing technologies often struggle to achieve both simultaneously.

[0004] For example, the spatial resolution of microdissection techniques is highly dependent on the precision of the cutting tool. Laser microdissection, by using a laser to precisely cut the target area, can obtain high-resolution samples. However, this technique also has some drawbacks: the heat generated during laser cutting may damage proteins in the sample, and existing laser microdissection techniques can only selectively cut and sample regions of interest, which poses a challenge to the overall protein expression pattern of the sample. Summary of the Invention

[0005] This application addresses the shortcomings of existing technologies by proposing a sample cutting mold and its sample collection method to solve the technical problems of existing technologies that cannot guarantee the integrity of biological cell samples as much as possible using micro-cutting techniques, and that may cause sample loss.

[0006] In a first aspect, embodiments of this application provide a sample cutting mold, including a cutting template;

[0007] The cutting template is equipped with several cutting blades that are in close contact with each other;

[0008] The cutting blade includes the blade body;

[0009] The blade is a three-dimensional polygonal prism, and the inner side of the blade has a through hole;

[0010] The first end of the blade serves as the cutting edge, which is polygonal in shape.

[0011] The second end of the blade serves as the sampling port; the blade edge and the sampling port are positioned opposite each other.

[0012] One possible implementation also includes at least one of the following:

[0013] The materials used for the cutting template include at least one: resin, ceramic, and metal.

[0014] The sample cutting mold can be manufactured in at least one of the following ways: by printing with micro-nano 3D printing equipment, by machining, by injection molding, and by producing with metal die casting molds.

[0015] In one possible implementation, the orthographic projection of the sampling port along the direction perpendicular to the cutting template covers the orthographic projection of the blade along the direction perpendicular to the cutting template.

[0016] The sampling port is a regular polygon.

[0017] In one possible implementation, the polygon includes at least one of the following: regular polygon, rectangle, and parallelogram.

[0018] In one possible implementation, the regular polygon includes at least one of the following: a regular hexagon, a square, and an equilateral triangle;

[0019] Each side of the polygon is no less than 0.01 mm and no more than 2 mm.

[0020] In one possible implementation, the regular polygon is a regular hexagon;

[0021] The side length of the regular hexagon is 0.225 mm, the diameter of the circumcircle of the regular hexagon is 0.45 mm, and the diameter of the incircle of the regular hexagon is 0.39 mm.

[0022] In one possible implementation, the cutting angle of the cutting blade is not less than 5 degrees and not more than 45 degrees.

[0023] In one possible implementation, the cutting angle of the cutting blade is 12.9 degrees.

[0024] In one possible implementation, the length of the cutting template is not less than 0.5 mm and not more than 500 mm;

[0025] The width of the cutting template shall be no less than 0.5 mm and no more than 500 mm;

[0026] The thickness of the cutting template shall be no less than 0.1 mm and no more than 5 mm.

[0027] Secondly, embodiments of this application provide a sample sampling method based on a sample cutting mold, the method comprising:

[0028] Place the biological sample in the predetermined position of the support device;

[0029] Align the sample cutting mold and place it above the biological sample, with the cutting edge of the sample cutting mold facing the biological sample;

[0030] The pressure device is fixed above the sample cutting mold;

[0031] By applying pressure to the pressurizing device, the pressurizing device moves in the pressing direction under the action of pressure, causing the cutting blade of the sample cutting mold to divide the biological sample and place the divided biological sample into the blade body of the cutting blade of the sample cutting mold.

[0032] The segmented biological sample is removed from the blade of the sample cutting mold.

[0033] This application also provides a sample cutting mold, which consists of a plurality of closely contacting cutting blades on a cutting template. Each cutting blade includes a blade body; the blade body is a three-dimensional polygonal prism with a through hole on its inner side; the first end of the blade body serves as a cutting edge, which is polygonal; the second end of the blade body serves as a sampling port; the cutting edge and the sampling port are positioned opposite each other. During the biological sample sampling process, pressure is applied to the sample cutting mold by a pressurizing device, causing the sample cutting mold to complete the cutting and sampling of the biological sample. Because the cutting template has a plurality of closely contacting cutting blades, multiple complete cut samples can be produced at once. Since laser micro-cutting technology is not required, the integrity of the biological cell sample is ensured as much as possible, and sample loss is avoided. Compared with high-cost laser micro-cutting equipment, the cost is reduced. In addition, the sample cutting mold provided in this application embodiment is compatible with automation, which can reduce manual operation and improve experimental efficiency and reproducibility. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the structure of a sample cutting mold provided in an embodiment of this application;

[0035] Figure 2 This is a schematic diagram of another sample cutting mold provided in an embodiment of this application;

[0036] Figure 3 Examples of embodiments of this application Figure 2 A schematic diagram of the structure of a single blade cutting unit;

[0037] Figure 4 This is a schematic diagram of a sample sampling method based on a sample cutting mold provided in an embodiment of this application.

[0038] Figure label:

[0039] 100 - Sample cutting mold, 1 - Cutting mold, 10 - Cutting blade, 11 - Blade body, 12 - Blade edge, 13 - Sampling port;

[0040] 200-Support device, 201-Positioning post, 202-Groove;

[0041] 300 - Pressurization device; 301 - Positioning hole; 302 - Raised block;

[0042] 500 - biological samples. Detailed Implementation

[0043] Various embodiments and features of this application are described herein with reference to the accompanying drawings.

[0044] It should be understood that various modifications can be made to the embodiments described herein. Therefore, the above description should not be considered as limiting, but merely as an example of embodiments. Other modifications within the scope and spirit of this application will be apparent to those skilled in the art.

[0045] The accompanying drawings, which are included in and form part of this specification, illustrate embodiments of the present application and, together with the general description of the present application given above and the detailed description of the embodiments given below, serve to explain the principles of the present application.

[0046] These and other features of this application will become apparent from the following description of preferred forms of embodiments given as non-limiting examples, with reference to the accompanying drawings.

[0047] It should also be understood that although this application has been described with reference to some specific examples, those skilled in the art can certainly implement many other equivalent forms of this application.

[0048] The above and other aspects, features and advantages of this application will become more apparent when taken in conjunction with the accompanying drawings and in view of the following detailed description.

[0049] Specific embodiments of this application are described thereafter with reference to the accompanying drawings; however, it should be understood that the claimed embodiments are merely examples of this application, which can be implemented in various ways. Well-known and / or repeated functions and structures are not described in detail to avoid unnecessary or redundant details that could obscure the application. Therefore, the specific structural and functional details claimed herein are not intended to be limiting, but merely serve as the basis and representative basis for the claims to teach those skilled in the art to use this application in a variety of substantially any suitable detailed structures.

[0050] This specification may use the phrases “in one embodiment,” “in another embodiment,” “in yet another embodiment,” or “in other embodiments,” all of which may refer to one or more of the same or different embodiments according to this application.

[0051] This application provides a sample cutting mold, combined with Figure 1 and Figure 2 As shown, the sample cutting mold 100 includes a cutting template 1; the cutting template 1 is provided with a plurality of cutting blades 10 that are in close contact with each other; the cutting blade 10 includes a blade body 11; the blade body 11 is a three-dimensional polygonal prism and the inner side of the blade body 11 is a through hole; the first end of the blade body 11 serves as a cutting edge 12, and the cutting edge 12 is polygonal; the second end of the blade body 11 serves as a sampling port 13; the cutting edge 12 and the sampling port 13 are arranged opposite to each other.

[0052] The sample cutting mold provided in this application embodiment has several cutting blades that are in close contact with each other on a cutting template. Each cutting blade includes a blade body; the blade body is a three-dimensional polygonal prism with a through hole on its inner side; the first end of the blade body serves as a cutting edge, which is polygonal; the second end of the blade body serves as a sampling port; the cutting edge and the sampling port are positioned opposite each other. During the biological sample sampling process, pressure is applied to the sample cutting mold by a pressurizing device, causing the sample cutting mold to complete the cutting and sampling of the biological sample. Because several cutting blades are in close contact with each other on the cutting template, multiple complete cut samples can be produced at once. Since laser micro-cutting technology is not required, the integrity of the biological cell sample is ensured as much as possible, sample loss is avoided, and costs are reduced. In addition, the sample cutting mold provided in this application embodiment is compatible with automated equipment, which can reduce manual operation and improve experimental efficiency and reproducibility.

[0053] The cutting blades of the sample cutting mold provided in this application are arranged in a polygonal tessellation pattern. This tessellation pattern not only ensures the integrity of the cut sample and avoids sample loss due to irregular arrangement, but also provides a high sample recovery rate, achieving 100% sample utilization. It is easy to manufacture and suitable for various polygonal shapes (such as regular hexagons, equilateral triangles, squares, rectangles, and parallelograms), adapting to different types of biological samples and experimental scenarios, thus improving practicality and versatility. Furthermore, while this application uses a tessellation pattern as the primary design mode, it also supports flexible adjustments to other arrangement patterns according to specific experimental needs.

[0054] In some embodiments, the material of the cutting template 1 includes at least one: resin material, ceramic material, and metal material. The resin material may include rigid resin, tough resin, high-temperature resistant resin, biocompatible resin, weather-resistant resin, etc.

[0055] For example, the resin material may include a photosensitive resin. For instance, the photosensitive resin is Mofang Precision HTL-Y high-temperature resistant photosensitive resin, which has advantages such as fast photocuring speed, smooth surface, and high processing precision, making it an ideal material for manufacturing high-precision molds at the micro-nano level. Sample cutting molds made with this material possess excellent mechanical properties and surface quality, effectively improving cutting accuracy and efficiency.

[0056] Metallic materials, especially special alloys resistant to acid and alkali corrosion, are relatively strong and durable.

[0057] Ceramic materials are a class of inorganic non-metallic materials made from natural or synthetic compounds through shaping and high-temperature sintering. They have advantages such as high melting point, high hardness, high wear resistance, and oxidation resistance.

[0058] In some embodiments, combined with Figure 2 and Figure 3 As shown, the orthographic projection of the sampling port 13 along the direction perpendicular to the cutting template 1 covers the orthographic projection of the cutting edge along the direction perpendicular to the cutting template 1; the outer diameter of the blade body 11 gradually increases from the cutting edge 12 to the sampling port 13. The sampling port 13 is a regular polygon.

[0059] In some embodiments, the polygon may include at least one: a regular polygon, a rectangle, and a parallelogram.

[0060] Optionally, a regular polygon includes at least one of a regular hexagon, a square, and an equilateral triangle.

[0061] Specifically, the cutting edges 12 of the sample cutting mold can all be regular hexagons (e.g., Figure 1 and Figure 2 As shown in the figure, the blades 12 of the sample cutting mold can all be equilateral triangles, squares, rectangles or parallelograms (not shown in the figure). Of course, they can also be other polygons, as long as each blade can make close contact and achieve complete sampling of biological cell samples. This application does not impose any special limitations.

[0062] The sample cutting mold of this application includes multiple cutting blades, and the blade edges of each cutting blade are arranged in a close-packed regular polygonal pattern. This arrangement can maximize sample utilization, reduce sample waste during the cutting process, improve the efficiency and completeness of the cutting effect, and adapt to high-throughput sampling requirements.

[0063] In some embodiments, the length of each side of the polygon is not less than 0.01 mm and not more than 2 mm. The length of the polygon can be flexibly adjusted according to experimental requirements, and this application does not impose any special limitations.

[0064] For example, the regular polygon is a regular hexagon; the side length of the regular hexagon is 0.225 mm, the diameter of the circumcircle of the regular hexagon is 0.45 mm, and the diameter of the incircle of the regular hexagon is 0.39 mm; the side length of the regular hexagon can also be other values, which are not specifically limited in this application.

[0065] In a specific example, the number of cutting blades 10 can be 711, but it can be adjusted to other numbers according to actual needs; this application does not impose any particular limitation. The sample cutting mold of this application can efficiently cut multiple samples simultaneously.

[0066] In some embodiments, see Figure 3 As shown, the cutting angle α of the cutting blade 10 is not less than 5 degrees and not greater than 45 degrees. Specifically, the cutting angle α of the cutting blade 10 is the included angle between two adjacent faces of one side of the blade edge 12 of the cutting blade 10, specifically the included angle between two adjacent faces of one side of a regular hexagon.

[0067] For example, the cutting angle α of the cutting blade 10 can be 12.9 degrees, 13 degrees, 20 degrees, etc., and this application does not make any special limitation.

[0068] In some embodiments, the outer contour of the cutting template 1 can resemble a cuboid, a cube, or the like.

[0069] For example, the length of the cutting template 1 is not less than 0.5 mm and not more than 500 mm;

[0070] The width of cutting template 1 is not less than 0.5 mm and not more than 500 mm;

[0071] The thickness of the cutting template 1 is not less than 0.1 mm and not more than 5 mm.

[0072] The size of the cutting template 1 can be adjusted according to the size of the biological sample; this application does not impose any special limitations.

[0073] In a specific example, the dimensions of the cutting template 1 are 12.00mm × 12.00mm × 1.60mm, the number of cutting blades 10 can be 711, the blade edge 12 is a regular hexagon with a side length of 0.225mm, and the cutting angle α of the cutting blade 10 can be 12.9 degrees, which can meet the cutting requirements of biological samples. It is suitable for the precise cutting of micro-samples, while ensuring the portability and ease of operation of the mold.

[0074] In some embodiments, the sample cutting mold is manufactured by printing using a micro / nano 3D printing device.

[0075] In other embodiments, the sample cutting mold can also be manufactured by machining, injection molding, or metal die casting.

[0076] Micro-nano 3D molding stands out for its flexibility and rapid prototyping capabilities, enabling the economical production of customized, complex-shaped parts. Machining, with its high precision and material properties, is suitable for small-batch, high-requirement part production. Injection molding is used for low-melting-point materials. Die casting is used for high-melting-point materials, exhibiting high efficiency, low cost, and strong consistency in mass production, making it suitable for medium- to long-term mass production.

[0077] Alternatively, micro-nano 3D printing equipment can be used, with Autodesk Fusion 360 as the design software and HTL-Y high-temperature resistant photosensitive resin as the printing material, to complete the fabrication of sample cutting molds.

[0078] This application addresses the technical bottlenecks in biological sample collection for space omics by proposing an innovative solution that enables high-throughput, high-resolution, and non-destructive cutting. Using the sample cutting mold of this application, hundreds of cut samples can be generated simultaneously as needed. Each sample is placed within a tightly contacting blade, and the cutting edge of each blade is a closely tessellated polygon with a circumcircle diameter within the range of several hundred micrometers. This ensures high resolution while avoiding sample loss, achieving a 100% sample recovery rate.

[0079] This application uses a high-temperature resistant photosensitive resin as the material for the sample cutting mold. This material boasts advantages such as rapid photosensitive curing, a smooth surface, and high precision, enabling the mold to achieve micro-nano-level processing accuracy, thereby significantly improving the cutting quality of small samples. Compared to common cutting rings or other cutting molds in the prior art, the sample cutting mold provided in this application is smaller in size and has higher processing precision, enabling non-destructive sampling. Furthermore, unlike existing technologies that may lead to partial sample loss or low recovery rates, this application ensures that the sample integrity is not affected during the cutting process.

[0080] For example, this application provides a sample cutting mold comprising 711 cutting blades, each with a hexagonal blade edge and an inscribed circle diameter of 300 micrometers, capable of completely and non-destructively cutting expanded gel samples (biological samples). The resolution of each cut sample reaches the level of dozens of cells, providing sufficient accuracy and reproducibility for spatial proteomics research. Furthermore, the sample cutting mold of this application supports flexible customization; its size, the close-packing shape of the cutting blade edges, and the number of cutting blades can all be adjusted according to actual needs.

[0081] The sample cutting mold provided in this application is integrally formed using micro-nano 3D printing technology, which is more suitable for high-precision manufacturing of small dimensions compared to traditional machining. 3D printing technology can precisely achieve micro-nano-level details, thus ensuring the mold's accuracy and surface smoothness. Furthermore, the cutting blade employs a closely spaced polygon design to maximize sample cutting utilization, guaranteeing a 100% sample recovery rate. Moreover, this closely spaced pattern reduces biological sample waste caused by insufficient shape design, making it a highly versatile sample cutting solution.

[0082] The sample cutting mold provided in this application is not only adaptable to various sample cutting scenarios but also compatible with automated equipment. By combining it with automated operation, cumbersome manual operation steps can be minimized, significantly improving the reproducibility and efficiency of experiments. This feature makes it particularly suitable for the high-throughput sample processing needs of large-scale space omics research.

[0083] The sample cutting mold provided in this application can achieve the following beneficial effects in terms of material selection, mold shape design, processing technology application, and sample processing efficiency:

[0084] 1. High-throughput and high-resolution non-destructive cutting capability: Supports complete cutting of samples at the hundreds of micrometer level and achieves 100% sample recovery rate;

[0085] 2. Advanced materials and manufacturing processes: HTL-Y resin combined with micro-nano 3D printing technology ensures the processing precision of the cutting mold;

[0086] 3. Flexibility and versatility: Supports customization of different sizes, shapes and numbers of holes to adapt to various experimental needs;

[0087] 4. Automation compatibility: Reduces manual operations and improves experimental efficiency and repeatability.

[0088] Based on the above characteristics, this invention provides an effective solution in space omics technology and opens up new avenues for efficient processing of biological samples.

[0089] Based on the same inventive concept, see [link to inventive concept] Figure 4 As shown in the embodiment of this application, a sample sampling method based on a sample cutting mold is provided, the method comprising:

[0090] Place the biological sample 500 in the predetermined position of the support device 200;

[0091] The sample cutting mold 100 is aligned and placed above the biological sample 500, wherein the cutting edge 12 of the cutting blade 10 of the sample cutting mold 100 faces the biological sample 500.

[0092] The pressure device 300 is fixed above the sample cutting mold 100;

[0093] By applying pressure to the pressurizing device 300, the pressurizing device 300 moves in the pressing direction under the action of pressure, so that the cutting blade 10 of the sample cutting mold 100 divides the biological sample 500 and places the divided biological sample into the blade body 11 of the cutting blade 10 of the sample cutting mold 100.

[0094] The segmented biological sample is removed from the blade body 11 of the cutting blade 10 of the sample cutting mold 100.

[0095] For details, see Figure 4 First, sample preparation: The biological sample 500 to be processed is placed stably in the center of the groove 202 of the support device 200, ensuring that the surface of the biological sample 500 is flat and stable, and avoiding the cutting effect caused by the movement of the biological sample 500 during the cutting process. The biological sample 500 in this application is used for space omics analysis.

[0096] Secondly, mold positioning: The sample cutting mold 100 is precisely aligned and placed above the biological sample 500, ensuring that the cutting edge 12 of the cutting blade 10 of the sample cutting mold 100 is in complete contact with the surface of the biological sample 500, and the sample cutting mold 100 is kept horizontal. At this time, the cutting edge 12 of the cutting blade 10 of the sample cutting mold 100 faces the surface of the biological sample 500, ensuring that the sample cutting mold 100 can effectively cut into the biological sample 500 to achieve the cutting of the biological sample 500. By aligning the sample cutting mold 100 and the biological sample 500, it is ensured that the sample cutting mold 100 acts evenly and stably on the biological sample 500 during the cutting process, preventing errors.

[0097] Next, pressure cutting: The four positioning holes 301 of the pressure device 300 are passed through the four positioning posts 201 of the support device 200 and guided to the top of the sample cutting mold 100. The protruding square 302 of the pressure device 300 falls precisely into the groove 202 of the support device 200, and applies uniform downward pressure towards the biological sample 500, so that the edge of the blade 12 of the cutting blade 10 of the sample cutting mold 10 accurately cuts into the biological sample 500. During the pressure application process, it should be ensured that the pressure is moderate to avoid damage or uneven cutting of the biological sample 500 due to excessive or insufficient pressure. During the cutting of the biological sample 500, the edge of the blade 12 of the cutting blade 10 of the sample cutting mold 100 will accurately divide the biological sample 500, so that it maintains the integrity and high resolution of the original biological sample 500 after division. The divided biological sample 500 is placed into the blade body 11 of the cutting blade 10 of the sample cutting mold 100.

[0098] Finally, sample collection: After the biological sample 500 is cut, the sample cutting mold 100 is carefully removed, and the segmented biological sample 500 is taken out one by one from the sampling port 13 of the blade 11 of the cutting blade 10 of the sample cutting mold 100. All cut samples retain the integrity of their original spatial information, and each cut sample is the same size, achieving high-resolution sampling. Moreover, during the cutting process of the biological sample 500, each cut sample can accurately correspond to the blade edge of the cutting blade, preserving the original feature information of the biological sample 500 to the maximum extent.

[0099] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. A sample cutting mold characterized by, Including cutting templates; The cutting template is provided with several cutting blades that are in close contact with each other; The cutting blade includes a blade body; The blade body is a three-dimensional polygonal prism, and the inner side of the blade body has a through hole; The first end of the blade body serves as the cutting edge, and the cutting edge is polygonal; The second end of the blade serves as a sampling port; the blade edge is positioned opposite to the sampling port.

2. The sample cutting die of claim 1, wherein, It also includes at least one of the following: The material of the cutting template includes at least one: resin material, ceramic material, and metal material; The sample cutting mold is manufactured by at least one of the following methods: printing with micro-nano 3D printing equipment, machining, injection molding, and production using metal die casting molds.

3. The sample cutting mold according to claim 1, characterized in that, The orthographic projection of the sampling port along the direction perpendicular to the cutting template covers the orthographic projection of the blade along the direction perpendicular to the cutting template. The sampling port is a regular polygon.

4. The sample cutting mold according to claim 1, characterized in that, The polygon includes at least one of the following: regular polygon, rectangle and parallelogram.

5. The sample cutting mold according to claim 4, characterized in that, The regular polygon includes at least one of the following: a regular hexagon, a square, and an equilateral triangle; Each side of the polygon has a length of not less than 0.01 mm and not more than 2 mm.

6. The sample cutting mold according to claim 5, characterized in that, The regular polygon is a regular hexagon; The regular hexagon has a side length of 0.225 mm, a circumcircle diameter of 0.45 mm, and an incircle diameter of 0.39 mm.

7. The sample cutting mold according to claim 1, characterized in that, The cutting angle of the cutting blade is not less than 5 degrees and not more than 45 degrees.

8. The sample cutting mold according to claim 7, characterized in that, The cutting angle of the cutting blade is 12.9 degrees.

9. The sample cutting mold according to claim 5, characterized in that, The length of the cutting template is not less than 0.5 mm and not more than 500 mm; The width of the cutting template is not less than 0.5 mm and not more than 500 mm; The thickness of the cutting template is not less than 0.1 mm and not more than 5 mm.

10. A sample sampling method based on the sample cutting mold according to any one of claims 1 to 9, characterized by, The method includes: Place the biological sample in the predetermined position of the support device; The sample cutting mold is aligned and placed above the biological sample, wherein the cutting edge of the sample cutting mold faces the biological sample; The pressure device is fixed above the sample cutting mold; By applying pressure to the pressurizing device, the pressurizing device moves in the pressing direction under the action of the pressure, causing the cutting blade of the sample cutting mold to divide the biological sample and place the divided biological sample into the blade body of the cutting blade of the sample cutting mold. The biological sample that has been segmented is removed from the blade of the cutting tool of the sample cutting mold.

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