Disposable biological reaction bag

Abstract

The utility model provides a disposable biological reaction bag, which relates to the technical field of reaction bags and comprises a reaction bag, a discharge pipe penetrates through the middle of the lower end of the reaction bag, a plurality of liquid inlet pipes penetrate through one side of the lower end of the reaction bag, and hanging grooves penetrate through two sides of the upper end of the front surface of the reaction bag respectively. A mixing mechanism is mounted in the reaction bag. After a user injects a culture solution into the reaction bag, the fixed block and the mixing frame can be driven by the rotating shaft to slowly rotate in the reaction bag, so that the culture solution and the cell sap are stirred, the contact and exchange of the culture solution and the cell sap are improved, and the growth efficiency of cells is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of reaction bags, in particular to a disposable biological reaction bag. Background Art

[0002] A disposable bioreactor bag is a device used for cell culture, usually made of plastic materials such as polyethylene or polypropylene. This equipment is usually used in research and production processes in the fields of biopharmaceuticals, cell therapy and other life sciences. A disposable bioreactor bag proposed in the publication number "CN201920345286.5" includes a bag body for storing culture fluid, and the bag body is connected to a liquid outlet component for outputting the culture fluid and a liquid inlet component for injecting the culture fluid into the bag body. The culture fluid for cell growth is injected into the bag body by the liquid inlet component, and the culture fluid in the bag body is discharged by the liquid outlet component, so that the cells can undergo biological reactions in the bag body. When the experiment is completed, the bag body can be connected and directly discarded, which greatly reduces the use of stainless steel tanks, saves costs, and is easy to clean.

[0003] However, in the above technical solution, the bioreactor bag only injects and discharges the culture solution into the bag. After the culture solution is injected into the reaction bag, natural convection or the movement of the cells themselves is relied on to promote the contact and exchange between the culture solution and the cell fluid. This method is inefficient and affects the growth rate of cells to a certain extent. Therefore, we propose a disposable bioreactor bag to solve the above problems. Utility Model Content

[0004] The main purpose of the utility model is to provide a disposable bioreactor bag, which solves the problem that after the culture fluid is injected into the reaction bag, the natural convection or the movement of the cells themselves is used to promote the contact and exchange between the culture fluid and the cell fluid. This method is inefficient and affects the growth rate of the cells to a certain extent.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A disposable biological reaction bag comprises a reaction bag, wherein a discharge pipe is installed through the middle of the lower end of the reaction bag, a plurality of liquid inlet pipes are installed through one side of the lower end of the reaction bag, hanging grooves are respectively installed on both sides of the upper end of the front surface of the reaction bag, a mixing mechanism is installed inside the reaction bag, the mixing mechanism comprises a rotating shaft, the rotating shaft is movably installed through the rear end of the reaction bag, a plurality of mixing racks are installed outside the shaft of the rotating shaft located inside the reaction bag, and a sealing block is installed between the rotating shaft and the reaction bag.

[0007] Preferably, sealing covers are respectively installed at the lower ends of the liquid inlet pipes.

[0008] Preferably, a connection block is fixedly installed at the rear end of the reaction bag, and the rotating shaft is movably installed through the interior of the connection block.

[0009] Preferably, a fixing block is fixedly mounted on the outside of the shaft where the rotating shaft is located inside the reaction bag, a plurality of mixing racks are mounted on the fixing block, and the fixing block and the mixing racks are movably mounted inside the reaction bag.

[0010] Preferably, a groove is provided on the side of the rotating shaft away from the mixing frame.

[0011] Preferably, a sealing block is fixedly installed on the outside of the shaft of the rotating shaft located inside the reaction bag, a sealing groove is provided on the surface of the connecting block, the sealing block is snap-fitted inside the sealing groove, and the ends of the sealing block and the sealing groove close to each other are both set as triangles.

[0012] Compared with the prior art, the utility model has the following beneficial effects:

[0013] (1) In the present invention, after the user injects the culture fluid into the reaction bag, the fixed block and the mixing frame can be driven by the rotating shaft to slowly rotate inside the reaction bag, thereby achieving the stirring of the culture fluid and the cell fluid, improving the contact and exchange between the culture fluid and the cell fluid, and increasing the growth efficiency of the cells.

[0014] (2) The utility model uses the groove to facilitate the user to engage the rotating shaft with the external transmission device, thereby achieving the effect of plug-and-play and pull-out-and-throw-away. It is convenient for the user to use the bioreactor bag and also convenient for the later disassembly and recycling of the bioreactor bag. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the overall structure of a disposable bioreactor bag of the utility model;

[0016] Figure 2 This is a front view structural diagram of a disposable bioreactor bag of the utility model;

[0017] Figure 3 The utility model is a disposable bioreactor bag Figure 2 Schematic diagram of the cross-section structure at point A in the middle;

[0018] Figure 4 The utility model is a disposable bioreactor bag Figure 3 The enlarged structural diagram at B in the middle;

[0019] Figure 5 The utility model is a disposable bioreactor bag Figure 4 Enlarged structural diagram at point C in the middle.

[0020] In the figure: 1, reaction bag; 2, hanging groove; 3, discharge pipe; 4, liquid inlet pipe; 5, sealing cover; 6, mixing mechanism; 601, rotating shaft; 602, connecting block; 603, groove; 604, fixing block; 605, mixing frame; 606, sealing groove; 607, sealing block. DETAILED DESCRIPTION

[0021] The following will be combined with the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] like Figures 1 to 5 As shown, the embodiment of the utility model proposes a disposable biological reaction bag, including a reaction bag 1, a discharge pipe 3 is installed through the middle of the lower end of the reaction bag 1, a plurality of liquid inlet pipes 4 are installed through one side of the lower end of the reaction bag 1, hanging grooves 2 are respectively installed on both sides of the upper end of the front surface of the reaction bag 1, a mixing mechanism 6 is installed inside the reaction bag 1, and the mixing mechanism 6 includes a rotating shaft 601, which is movably installed through the rear end of the reaction bag 1, and a plurality of mixing frames 605 are installed outside the shaft of the rotating shaft 601 located inside the reaction bag 1, and a sealing block 607 is installed between the rotating shaft 601 and the reaction bag 1.

[0023] like Figures 2 to 5 As shown, in another embodiment of the utility model, a sealing cover 5 is respectively installed at the lower end of the liquid inlet pipe 4, a connecting block 602 is fixedly installed at the rear end of the reaction bag 1, and a rotating shaft 601 is movably installed in the interior of the connecting block 602. A fixing block 604 is fixedly installed on the outside of the shaft of the rotating shaft 601 located in the reaction bag 1, and a plurality of mixing racks 605 are installed on the fixing block 604. The fixing block 604 and the mixing rack 605 are movably installed in the interior of the reaction bag 1. A groove 603 is provided on the side of the rotating shaft 601 away from the mixing rack 605. A sealing block 607 is fixedly installed on the outside of the shaft of the rotating shaft 601 located in the reaction bag 1. A sealing groove 606 is provided on the surface of the connecting block 602. The sealing block 607 is snap-fitted and installed in the interior of the sealing groove 606. The ends of the sealing block 607 and the sealing groove 606 that are close to each other are both set as triangles.

[0024] The reaction bag 1 and the mixing mechanism 6 are both made of polyethylene material. The lightness and strength of polyethylene material make the overall weight of the reaction bag lighter, and the density is one tenth of the density of metal. Some enhanced reaction bags even have a strength close to or higher than that of steel. In addition, the chemical properties are stable and the friction and wear resistance are excellent, making them more convenient to use.

[0025] The rotating shaft 601 is connected to an external transmission device, such as a motor or an object such as an inner hexagonal wrench, through the groove 603. If the motor is connected to the rotating shaft 601 through the groove 603, the culture fluid and the cell fluid can be automatically stirred. If the inner hexagonal wrench is connected to the rotating shaft 601 through the groove 603, the user can slowly stir the cell fluid according to the strength and speed required for stirring the cell fluid, thereby better protecting the cell fluid.

[0026] The sealing performance is improved by setting the engaging surface between the sealing block 607 and the sealing groove 606 to be triangular. The triangular engaging surface can better adapt to the shape difference between the sealing block 607 and the sealing groove 606, thereby reducing the possibility of leakage.

[0027] The working principle of a disposable bioreactor bag:

[0028] When in use, the user first lifts the reaction bag 1 through the hanging groove 2, and then the user injects the culture solution or cell solution into the reaction bag 1 through the liquid inlet tube 4. Then, when the inside of the reaction bag 1 needs to be mixed and stirred, if the rotating shaft 601 is connected to the motor through the groove 603, the culture solution and cell solution can be automatically stirred by the motor. If the rotating shaft 601 is connected to an object such as an Allen wrench through the groove 603, the user can slowly stir the cell solution according to the required stirring force and speed.

[0029] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not limitations on the implementation methods of the present invention. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is impossible to list all the implementation methods here. All obvious changes or modifications derived from the technical solution of the present invention are still within the protection scope of the present invention.

Claims

1. A disposable bioreactor bag, comprising a reaction bag (1), characterized in that: A discharge pipe (3) is installed through the middle of the lower end of the reaction bag (1), a plurality of liquid inlet pipes (4) are installed through one side of the lower end of the reaction bag (1), and hanging grooves (2) are respectively installed on both sides of the upper end of the front surface of the reaction bag (1). A mixing mechanism (6) is installed inside the reaction bag (1), and the mixing mechanism (6) includes a rotating shaft (601), and the rotating shaft (601) is movably installed through the rear end of the reaction bag (1). A plurality of mixing frames (605) are installed outside the shaft of the rotating shaft (601) located inside the reaction bag (1), and a sealing block (607) is installed between the rotating shaft (601) and the reaction bag (1).

2. A disposable bioreactor bag according to claim 1, characterized in that: The lower ends of the liquid inlet pipes (4) are respectively provided with sealing covers (5).

3. A disposable bioreactor bag according to claim 1, characterized in that: A connection block (602) is fixedly installed at the rear end of the reaction bag (1), and the rotating shaft (601) is movably installed in the connection block (602).

4. A disposable bioreactor bag according to claim 3, characterized in that: The rotating shaft (601) is located inside the reaction bag (1) and a fixed block (604) is fixedly installed on the outside of the rod body. A plurality of mixing racks (605) are installed on the fixed block (604). The fixed block (604) and the mixing racks (605) are movably installed inside the reaction bag (1).

5. A disposable bioreactor bag according to claim 3, characterized in that: A groove (603) is provided on the side of the rotating shaft (601) away from the mixing frame (605).

6. A disposable bioreactor bag according to claim 3, characterized in that: The rotating shaft (601) is located inside the reaction bag (1) and a sealing block (607) is fixedly installed on the outside of the rod body. The surface of the connecting block (602) is provided with a sealing groove (606). The sealing block (607) is snap-fitted and installed inside the sealing groove (606). The ends of the sealing block (607) and the sealing groove (606) that are close to each other are both set to be triangular.

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