Stem cell cryopreservation bag

Abstract

The utility model relates to the technical field of medical cryopreservation bags, in particular to a stem cell cryopreservation bag which comprises a bag body provided with a liquid containing cavity. The liquid inlet pipe and the exhaust pipe are located on the two opposite sides of the bag body respectively, and the liquid inlet pipe and the exhaust pipe are both connected with the bag body and communicate with the liquid containing cavity; the liquid drainage plug is connected with the bag body, and the liquid drainage plug is communicated with the side, away from the liquid inlet pipe, of the liquid containing cavity; the liquid inlet pipe can be matched with the liquid passing channel and the liquid containing cavity to form a one-way stem cell liquid flowing system, and an additional stem cell liquid temporary storage space can be provided for continuously injecting stem cell liquid to remove bubbles in the liquid containing cavity and the liquid passing channel through the exhaust pipe, so that manual exhaust is avoided; and the influence of the bubbles on the stem cell liquid perfusion bagging speed is reduced.

Description

Technical Field

[0001] The present application relates to the technical field of medical cryopreservation bags, and in particular to a stem cell cryopreservation bag. Background Art

[0002] A stem cell cryopreservation bag is a container specifically designed for safe storage and transportation of stem cells. These bags are typically made of biocompatible materials and are designed to protect stem cells from damage at extremely low temperatures (usually liquid nitrogen temperatures, about -196°C), ensuring cell viability and quality. Cryopreservation bags may contain specially designed sealing systems to prevent leaks and may be equipped with labels and barcodes for easy sample management and tracking.

[0003] The prior art discloses a stem cell cryopreservation bag suitable for cell reinfusion, which comprises a first storage bag body and 2 to 6 second storage bag bodies arranged side by side with the first storage bag body; a bag body connecting pipeline is arranged between the first storage bag body and the second storage bag body, and between each second storage bag body, so that a biological sample can flow from one bag body to another bag body; an input port is arranged on the upper edge of the first storage bag body and is connected to one end of a connecting pipe through the input port, and the biological sample enters the first storage bag body via the connecting pipe and the input port; a first sampling port is arranged on the upper edge of the first storage bag body, and a second sampling port is arranged on the upper edge of each second storage bag body, and the first sampling port and the second sampling port are used to take out the biological sample stored in the bag body.

[0004] With respect to the above-mentioned related technologies, when the cells to be frozen are input into multiple storage bags from the input port, the bag body of the storage bag will expand as the stem cell solution is injected, and bubbles are easily generated in the bag body cavity of the storage bag. The gas inside the generated bubbles will expand rapidly during the freezing and thawing process, thereby causing the bag to burst. Therefore, the generated bubbles need to be squeezed out manually during the perfusion stage, and the efficiency of manually discharging the bubbles is low, which affects the speed of perfusion and bagging of the stem cell solution. Utility Model Content

[0005] In order to reduce the impact of bubbles on the filling and bagging speed of stem cell solution, the present application provides a stem cell cryopreservation bag.

[0006] The present application provides a stem cell cryopreservation bag using the following technical solution:

[0007] A stem cell freezing bag, comprising:

[0008] A bag body, wherein the bag body has a liquid containing cavity;

[0009] A liquid inlet pipe and an exhaust pipe, the liquid inlet pipe and the exhaust pipe are respectively located at two opposite sides of the bag body, the liquid inlet pipe and the exhaust pipe are both connected to the bag body and communicated with the liquid containing cavity;

[0010] A liquid drain plug is connected to the bag body and communicated with a side of the liquid containing cavity away from the liquid inlet pipe.

[0011] By adopting the above technical scheme, the stem cell liquid to be frozen is injected into the liquid containing cavity through the liquid inlet pipe. With the injection of the stem cell liquid, the liquid containing cavity is gradually saturated. The stem cell liquid is continuously injected into the liquid inlet pipe until the liquid containing cavity is filled with the stem cell liquid. At this time, the stem cell liquid is continuously injected, and the stem cell liquid enters the exhaust pipe. The bubbles generated in the liquid containing cavity enter the exhaust pipe along the flow direction of the stem cell liquid. After all the bubbles in the liquid containing cavity are completely eliminated, the liquid inlet pipe and the exhaust pipe are sealed by high-frequency welding near the bag body. The designed stem cell freezing bag can form a liquid containing cavity through the bag body, thereby providing a storage space for the stem cell liquid. The exhaust pipe can provide an additional temporary storage space for the stem cell liquid for continuously injecting the stem cell liquid to eliminate the bubbles in the liquid containing cavity, thereby avoiding manual exhaust and reducing the influence of bubbles on the filling and bagging speed of the stem cell liquid. The drain plug can be used to discharge the stem cell liquid after the cryopreservation is thawed.

[0012] In a specific implementation manner, there are multiple liquid containing cavities, which are arranged in sequence, and the bag body has at least one liquid passage, and both ends of the liquid passage are respectively connected to two adjacent liquid containing cavities.

[0013] By adopting the above technical scheme, the stem cell liquid to be frozen is injected into the liquid containing cavity through the liquid inlet pipe. With the injection of the stem cell liquid, the liquid containing cavity is gradually saturated and under the action of the liquid pressure, the stem cell liquid enters the next adjacent liquid containing cavity through the liquid passage. The stem cell liquid is continuously injected into the liquid inlet pipe until all the liquid containing cavities are filled with the stem cell liquid. At this time, the stem cell liquid is continuously injected, and the stem cell liquid enters the exhaust pipe. The bubbles generated in the liquid containing cavity or the liquid passage enter the exhaust pipe along the direction of liquid flow. After all the bubbles in the liquid containing cavity and the liquid passage are completely eliminated, the liquid inlet pipe and the exhaust pipe are closed by high-frequency welding near the bag body and the middle area of ​​the liquid passage. The bag body designed with multiple liquid containing cavities can meet the accommodation requirements of stem cell liquids of different volumes. The liquid inlet pipe can cooperate with the liquid passage and the liquid containing cavity to form a unidirectional stem cell liquid flow system.

[0014] In a specific possible implementation manner, the liquid passage is located on a side of the liquid containing chamber away from the liquid drain plug.

[0015] By adopting the above technical solution, the liquid passage channel designed on the side of the liquid containing cavity away from the liquid discharge plug can reduce the possibility of bubbles flowing in the direction opposite to the flow direction of the stem cell liquid.

[0016] In a specific possible implementation manner, the communication width between the liquid inlet end of the liquid passage and the liquid containing chamber is greater than the communication width between the liquid outlet end of the liquid passage and the liquid containing chamber.

[0017] By adopting the above technical solution, the designed liquid passageway with a variable width in communication with the liquid containing cavity is conducive to the bubbles generated in the liquid containing cavity moving to the exhaust pipe along the flow direction of the stem cell liquid.

[0018] In a specific possible implementation manner, at least two hanging holes are provided on the bag body, and the hanging holes are provided on a side of the liquid containing cavity away from the liquid drain plug.

[0019] By adopting the above technical solution, the designed hanging hole can facilitate the bag body to be hung in a freezing device or a rewarming device.

[0020] In a specific embodiment, there is an unconnected area between two adjacent liquid containing chambers.

[0021] By adopting the above technical solution, the designed unconnected area can reduce the workload of high-frequency welding of the bag body while forming the liquid cavity, and can also reduce the welding area by reducing the working area of ​​high-frequency welding, thereby reducing the possibility of flowing plastic moving into the liquid cavity.

[0022] In a specific possible implementation manner, two overflow process holes are provided on the bag body, the liquid containing cavity is located between the two overflow process holes, and the overflow process holes are arranged close to the drain plug.

[0023] By adopting the above technical solution and designing the overflow process hole, the possibility of the flowing plastic moving into the liquid containing cavity can be reduced through the overflow process hole due to the large welding area at the edge of the bag body.

[0024] In a specific possible implementation manner, the communication width between the exhaust pipe and the liquid containing cavity gradually increases toward the side of the drain plug.

[0025] By adopting the above technical solution, the exhaust pipe is designed with a width connected to the liquid containing cavity gradually increasing toward the drain plug, which is conducive to the bubbles generated in the liquid containing cavity moving into the exhaust pipe along the flow direction of the stem cell liquid.

[0026] In summary, the present application includes at least one of the following beneficial technical effects:

[0027] 1. The designed stem cell cryopreservation bag can form a liquid cavity through the bag body, thereby providing a storage space for the stem cell liquid. The exhaust pipe can provide an additional temporary storage space for the stem cell liquid for continuous injection of stem cell liquid to remove bubbles in the liquid cavity, thereby avoiding manual exhaust and reducing the influence of bubbles on the filling and bagging speed of the stem cell liquid. The drain plug can be used to discharge the stem cell liquid after thawing.

[0028] 2. The designed stem cell cryopreservation bag can reduce the workload of high-frequency welding of the bag body while forming a liquid-containing cavity through the unconnected area, and can also reduce the welding area by reducing the working area of ​​high-frequency welding, thereby reducing the possibility of flowing plastic moving into the liquid-containing cavity.

[0029] 3. The designed stem cell cryopreservation bag is facilitated to move bubbles generated in the liquid containing cavity into the exhaust pipe along the flow direction of the stem cell liquid through the exhaust pipe which is connected to the liquid containing cavity and has a width gradually increasing toward the drain plug. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a schematic diagram of the structure of the stem cell cryopreservation bag in Example 1 of the present application.

[0031] Figure 2 It is a schematic diagram of the structure of the stem cell cryopreservation bag in Examples 2, 3 and 4 of the present application.

[0032] Figure 3 It is a schematic diagram of the structure of the stem cell cryopreservation bag in Example 5 of the present application.

[0033] Figure 4 It is a schematic diagram of the structure of the stem cell cryopreservation bag in Example 6 of the present application.

[0034] Figure 5 It is a schematic diagram of the structure of the stem cell cryopreservation bag in Examples 7 and 8 of the present application.

[0035] Explanation of the accompanying reference numerals: 1. bag body; 11. liquid containing cavity; 12. liquid passage; 2. liquid inlet pipe; 3. exhaust pipe; 4. drain plug; 5. hanging hole; 6. unconnected area; 7. overflow process hole. DETAILED DESCRIPTION

[0036] The following is combined with Figure 1-5 This application is described in further detail.

[0037] The embodiment of the present application discloses a stem cell cryopreservation bag.

[0038] Example 1

[0039] Reference Figure 1A stem cell freezing bag includes a bag body 1, a liquid inlet pipe 2 and an exhaust pipe 3. The bag body 1 is provided with a liquid containing cavity 11. The liquid inlet pipe 2 and the exhaust pipe 3 are respectively located on opposite sides of the bag body 1. The liquid inlet pipe 2 and the exhaust pipe 3 are both connected to the bag body 1, and the inner cavities of the liquid inlet pipe 2 and the exhaust pipe 3 are both connected to the liquid containing cavity 11. The exhaust pipe 3 can provide an additional temporary storage space for stem cell liquid for continuously injecting stem cell liquid to eliminate bubbles in the liquid containing cavity 11, thereby avoiding manual exhaust and reducing the influence of bubbles on the filling and bagging speed of the stem cell liquid.

[0040] Reference Figure 1 In order to facilitate the discharge of the stem cell fluid in the liquid containing chamber 11 after freezing and thawing, and to reduce the possibility of the stem cell fluid being contaminated by the outside world, the stem cell freezing bag also includes a drain plug 4, which is connected to the bag body 1, and the drain plug 4 is connected to the side of the liquid containing chamber 11 away from the liquid inlet pipe 2; in the present application, the number of drain plugs 4 and the number of liquid containing chambers 11 are consistent and are arranged one by one, and the drain plug 4 can be set as a break plug or a twist-off plug. In the present embodiment, the drain plug 4 is preferably in the form of a twist-off plug.

[0041] Example 2

[0042] Reference Figure 2 On the basis of Example 1, in order to increase the storage demand for different volumes of stem cell fluid, the number of liquid containing chambers 11 on the bag body 1 is multiple, and the multiple liquid containing chambers 11 are in the same horizontal area, and the bag body 1 has at least one liquid passage 12, and the two ends of the liquid passage 12 are respectively connected to two adjacent liquid containing chambers 11; in the present application, the number of liquid containing chambers 11 on the bag body 1 can be two, three, or four. In the present embodiment, the number of liquid containing chambers 11 on the bag body 1 is five.

[0043] Example 3

[0044] Reference Figure 2 On the basis of Example 2, in order to reduce the possibility of bubbles flowing in the opposite direction of the stem cell fluid flow, the liquid passage 12 is located on the side of the liquid containing chamber 11 away from the drain plug 4, so that the distance between the connection between the liquid passage 12 and the liquid containing chamber 11 and the drain plug 4 is the shortest. In the present application, the liquid passage 12 can be set to an inverted "U" shape, a semi-arc shape, or other shapes. In the present embodiment, the liquid passage 12 is preferably set to an inverted "U" shape. After the stem cell fluid is perfused and the bubbles are discharged, high-frequency welding is required in the middle area of ​​the liquid passage 12 to separate the two adjacent liquid containing chambers 11.

[0045] Example 4

[0046] Reference Figure 2On the basis of Example 3, in order to facilitate the bubbles generated in the liquid containing chamber 11 to move into the exhaust pipe 3 along the flow direction of the stem cell liquid, the connecting width between the liquid inlet end of the liquid passage 12 and the liquid containing chamber 11 is greater than the connecting width between the liquid outlet end of the liquid passage 12 and the liquid containing chamber 11.

[0047] Example 5

[0048] Reference Figure 3 In this embodiment, based on any one of Embodiments 2 to 4, in order to facilitate hanging the bag body 1 in a freezing device or a thawing device, at least two hanging holes 5 are provided on the bag body 1. The hanging holes 5 are provided at one end of the liquid containing cavity 11 away from the drain plug 4, and the hanging holes 5 are located between the liquid outlet end and the liquid inlet end of two adjacent liquid passages 12.

[0049] Example 6

[0050] Reference Figure 4 In this embodiment, based on any one of the embodiments 1 to 4, in order to reduce the workload of high-frequency welding of the bag body 1 while forming the liquid containing cavity 11, and also to reduce the working area of ​​high-frequency welding and the welding area, thereby reducing the possibility of the flowing plastic moving into the liquid containing cavity 11, an unconnected area 6 is provided between two adjacent liquid containing cavities 11, and the bag body 1 includes two base films, which are connected by high-frequency welding, and structures such as the liquid containing cavity 11 or the liquid passage 12 are formed at the unconnected portion, and high-frequency welding is not performed in the area where the unconnected area 6 is located.

[0051] Example 7

[0052] Reference Figure 5 In this embodiment, based on any one of Embodiments 2 to 4, since the welding area at the edge of the bag body 1 is large, the possibility of the flowing plastic moving into the liquid containing cavity 11 can be reduced through the overflow process hole 7. Therefore, two overflow process holes 7 are further provided on the bag body 1, and the liquid containing cavity 11 is located between the two overflow process holes 7, and the overflow process hole 7 is arranged close to the drain plug 4.

[0053] Example 8

[0054] Reference Figure 5 Based on any one of Embodiments 1 to 4, this embodiment further discloses that the communication width between the exhaust pipe 3 and the liquid containing chamber 11 gradually increases toward the drain plug 4 in order to facilitate the bubbles generated in the liquid containing chamber 11 to move into the exhaust pipe 3 along the flow direction of the stem cell liquid.

[0055] The implementation principle of a stem cell cryopreservation bag in an embodiment of the present application is as follows: the stem cell liquid to be frozen is injected into the liquid containing chamber 11 through the liquid inlet pipe 2. With the injection of the stem cell liquid, the liquid containing chamber 11 is gradually saturated and under the action of the liquid pressure, the stem cell liquid enters the next adjacent liquid containing chamber 11 through the liquid flow channel 12. The stem cell liquid is continuously injected into the liquid inlet pipe 2 until all the liquid containing chambers 11 are filled with the stem cell liquid. At this time, the stem cell liquid is continuously injected, and the stem cell liquid enters the exhaust pipe 3. The bubbles generated in the liquid containing chamber 11 or the liquid flow channel 12 enter the exhaust pipe 3 along the liquid flow direction. After all the bubbles in the liquid containing chamber 11 and the liquid flow channel 12 are completely eliminated, the liquid inlet pipe 2 and the exhaust pipe 3 are sealed by high-frequency welding near the bag body 1 and the middle area of ​​each liquid flow channel 12.

[0056] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A stem cell cryopreservation bag, characterized in that: include: A bag body (1), wherein the bag body (1) is provided with a liquid containing cavity (11); a liquid inlet pipe (2) and an exhaust pipe (3), wherein the liquid inlet pipe (2) and the exhaust pipe (3) are respectively located at opposite sides of the bag body (1), and the liquid inlet pipe (2) and the exhaust pipe (3) are both connected to the bag body (1) and communicated with the liquid containing cavity (11); A liquid drain plug (4), the liquid drain plug (4) being connected to the bag body (1), and the liquid drain plug (4) being in communication with a side of the liquid containing cavity (11) away from the liquid inlet pipe (2); The communication width between the exhaust pipe (3) and the liquid containing chamber (11) gradually increases towards the side of the liquid drain plug (4).

2. The stem cell cryopreservation bag according to claim 1, characterized in that: The number of the liquid containing chambers (11) is multiple, and the multiple liquid containing chambers (11) are arranged in sequence, and the bag body (1) has at least one liquid passage (12), and the two ends of the liquid passage (12) are respectively connected to two adjacent liquid containing chambers (11).

3. The stem cell cryopreservation bag according to claim 2, characterized in that: The liquid passage (12) is located on a side of the liquid containing cavity (11) away from the liquid drain plug (4).

4. The stem cell cryopreservation bag according to claim 3, characterized in that: The communication width between the liquid inlet end of the liquid passage (12) and the liquid containing cavity (11) is greater than the communication width between the liquid outlet end of the liquid passage (12) and the liquid containing cavity (11).

5. The stem cell cryopreservation bag according to any one of claims 2 to 4, characterized in that: At least two hanging holes (5) are provided on the bag body (1), and the hanging holes (5) are provided on a side of the liquid containing cavity (11) away from the liquid drain plug (4).

6. The stem cell cryopreservation bag according to any one of claims 1 to 4, characterized in that: An unconnected area (6) is provided between two adjacent liquid containing chambers (11).

7. The stem cell cryopreservation bag according to any one of claims 2 to 4, characterized in that: The bag body (1) is provided with two overflow process holes (7), the liquid containing cavity (11) is located between the two overflow process holes (7), and the overflow process hole (7) is arranged close to the liquid drain plug (4).

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