Efficient oocyte collection vessel

Abstract

The utility model discloses a high-efficiency oocyte collection dish, which comprises a dish body and a dish cover covering the dish body, a detachable mesh screen for enriching oocytes is added in the culture dish, and the diameter of a liquid level through hole is controlled to enable a part of follicular fluid containing blood cells to flow out, but the oocytes cannot flow out, so that the oocytes can be collected more efficiently. The height of the detection liquid level is controlled by controlling the distance between the center of the liquid level through hole and the lower bottom surface of the mesh screen, and the diameter of the lower bottom surface of the mesh screen is controlled to be basically the same as that of an eyepiece of a microscope, so that the oocytes are accurately and quickly detected, and the influence of in-vitro exposure on the oocytes is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of assisted reproduction, in particular to a high-efficiency oocyte collection dish. Background Art

[0002] Embryo engineering refers to a variety of microscopic operations and processing techniques for early animal embryos or gametes, including in vitro fertilization, embryo transfer, embryo splitting and transfer, embryonic stem cell culture and other technologies. Embryo engineering is actually a simulated operation of natural fertilization and early embryonic development conditions of animals under in vitro conditions. The key link in embryo engineering technology is the collection and acquisition of oocytes. The most common method for collecting oocytes is superovulation, injecting gonadotropin hormone into the donor to make the donor discharge several to ten times more eggs than natural conditions at one time, and then flushing the eggs from the fallopian tubes, examining them under a microscope, selecting them, and then artificially culturing them in vitro to make them mature. Or collect oocytes from the ovaries, use ultrasound detectors (B-ultrasound), endoscopes and laparoscopes to determine the location of follicles, directly aspirate follicular fluid from living animals, take them out together with oocytes, examine them under a microscope, select them, and then artificially culture them in vitro to make them mature.

[0003] However, whether it is superovulation or collecting oocytes from the ovary, the oocytes obtained are all a kind of oocyte complex, which is mainly composed of granulosa cells (including oocytes), and inevitably contains a small amount of blood cells, hormones, cytokines and other substances. At present, assisted reproductive technology has not uniformly used oocyte collection special dishes, and each medical institution purchases disposable ordinary round plastic culture dishes on its own, and brands and models are different. In actual operation, the operator observes under a stereo microscope and finds that follicular fluid is poured into the ordinary culture dish. With inertia or tension, most of the contents will be distributed in the outermost edge area of ​​the dish, or the follicular fluid taken out is too thick, which is very unfavorable for the operator's microscopic observation. The operator is often required to adjust the best observation position by manually moving the ordinary culture dish continuously, which will greatly increase the operation time, and the time of oocyte exposure in vitro will be too long, and the excessive exposure time in vitro will have an adverse effect on oocytes, thereby affecting the success rate of assisted reproductive technology. At present, there are also some special cell culture dishes with filtering functions, such as: Chinese Patent 202021158560.7, but the filter in this design is placed above the culture dish, and it does not contact the cells in the culture dish. The main function of the filter is to filter the air to prevent the influence of microorganisms in the air on the culture in the dish during the frequent opening of the dish cover. The purpose of this utility model is to design an efficient oocyte collection dish, the culture dish has a built-in mesh screen, and the waste liquid such as blood cells in the follicular fluid is filtered through the mesh screen, so as to enrich the oocytes, and facilitate accurate and rapid observation of the oocytes remaining in the mesh screen. Utility Model Content

[0004] In order to solve the above-mentioned technical problems, the utility model provides an efficient oocyte collection dish. The dish has a built-in mesh screen, which is used to filter waste fluid such as blood cells in the follicular fluid, thereby enriching oocytes. At the same time, the liquid level is controlled to facilitate accurate and rapid observation of the oocytes remaining in the mesh screen.

[0005] The utility model solves the above-mentioned technical problems as follows:

[0006] An efficient oocyte collection dish comprises a dish body and a dish cover covering the dish body. A detachable mesh screen for enriching oocytes is installed inside the dish body. The mesh screen is in the shape of an inverted truncated cone with an open top surface. The diameter of the upper bottom surface of the mesh screen is 50-60 mm, and the diameter of the lower bottom surface of the mesh screen is 30-35 mm. A liquid surface through hole for allowing a part of follicular fluid containing blood cells to flow out is arranged on the side of the mesh screen. The diameter of the liquid surface through hole is 20-80 μm, and the distance between the center of the liquid surface through hole and the lower bottom surface of the mesh screen is 3-5 mm.

[0007] The invention concept of the utility model: the inventor found in the actual work process that there is no special dish for collecting oocytes in the field of assisted reproduction, and all ordinary culture dishes of different models are used. It is well known that follicular fluid is mainly composed of granulosa cells (including oocytes), and also contains a small amount of blood cells, hormones, cytokines and other substances. When the follicular fluid is poured into an ordinary culture dish, with the effect of inertia or tension, most of the contents (including oocytes) will be distributed in the outermost edge area of ​​the dish. Therefore, during the microscopic observation process, the operator needs to adjust the best observation position by manually moving the ordinary culture dish continuously, which will greatly increase the operation time, and will also cause the oocytes to be exposed in vitro for too long, and the in vitro exposure time is too long and will have an adverse effect on the oocytes. The inventor also tried to reduce the culture dish, and the size of the culture dish is basically the same as the microscope eyepiece, so that the oocytes can be gathered under the eyepiece, avoiding the operator to manually move the ordinary culture dish continuously to adjust the best observation position. However, as mentioned above, follicular fluid is a complex, and the thickness of follicular fluid and blood cells in the follicular fluid will affect the observation of oocytes. Therefore, simply adjusting the size of the culture dish cannot control the page height of follicular fluid and reduce the influence of blood cells and other substances in the follicular fluid on the detection. Therefore, the inventors thought of adding a mesh screen inside the culture dish and controlling the diameter of the liquid surface through hole to allow a part of the follicular fluid containing blood cells to flow out while the oocytes cannot flow out, thereby enriching the oocytes, and controlling the height of the detection liquid surface by controlling the distance between the center of the liquid surface through hole and the bottom surface of the mesh screen, and controlling the bottom surface diameter of the mesh screen to be basically the same as the eyepiece of the microscope, thereby achieving accurate and rapid detection of oocytes and reducing the influence of in vitro exposure on oocytes.

[0008] Furthermore, the liquid surface through holes are evenly distributed in a circle along the central axis of the mesh screen.

[0009] Furthermore, a circle of accelerating through holes evenly distributed along the central axis of the mesh is arranged above the liquid level through hole, and the distance between the center of the accelerating through hole and the bottom surface of the mesh is 8-10 mm. The main purpose of the accelerating through hole is to accelerate the outflow speed of the follicular fluid, and the main purpose of the liquid level through hole is to control the liquid level of the follicular fluid after the oocyte is enriched, and the liquid level is the optimal detection liquid level.

[0010] Furthermore, the upper bottom surface of the mesh screen has an outer edge extending outwards for easy gripping.

[0011] Furthermore, the diameter of the dish body is 80-90 mm. The space between the dish body and the mesh screen is a storage area for waste fluid (follicular fluid containing blood cells).

[0012] Furthermore, the bottom of the dish body is provided with four arc protrusions along the central axis of the dish body, and the lower end of the side of the mesh screen is clamped in the arc protrusions. The main function of the arc protrusion is to clamp the mesh screen to prevent the mesh screen from shaking in the dish body. The bottom surface of the mesh screen can be open or closed.

[0013] Furthermore, the mesh material is at least one of PE, PP, PET, PS, SB, SBC, PVA and medical nylon mesh.

[0014] Furthermore, the dish body is made of glass or plastic.

[0015] The utility model has the following advantages over the prior art:

[0016] 1. The utility model provides an efficient oocyte collection dish, by adding a detachable mesh screen for enriching oocytes in the culture dish, and controlling the diameter of the liquid surface through hole to allow a part of the follicular fluid containing blood cells to flow out while the oocytes cannot flow out, thereby enriching the oocytes, and controlling the height of the detection liquid level by controlling the distance between the center of the liquid surface through hole and the bottom surface of the mesh screen, and controlling the bottom surface diameter of the mesh screen to be basically the same as the microscope eyepiece, thereby achieving accurate and rapid detection of oocytes and reducing the impact of in vitro exposure on oocytes.

[0017] 2. The mesh screen of the utility model is designed with liquid surface through holes and acceleration through holes. The main purpose of the acceleration through holes is to accelerate the outflow rate of follicular fluid, and the main purpose of the liquid surface through holes is to control the liquid level of the follicular fluid after enrichment of oocytes, and the liquid level is the optimal detection liquid level.

[0018] 3. The dish body of the utility model is provided with four arc protrusions along the central axis of the dish body, and the lower end of the side of the mesh screen is clamped in the arc protrusion. The installation and removal of the mesh screen are simple and fast, which is convenient for cleaning the efficient oocyte collection dish.

[0019] 4. The mesh screen of the utility model is made of at least one of PE, PP, PET, PS, SB, SBC, PVA and medical nylon mesh. Therefore, the mesh screen is not too soft and has a certain deformation space, which is conducive to the installation and removal of the mesh screen.

[0020] Terms such as "upper" and "lower" used herein to indicate spatial relative positions are used for the purpose of convenience to describe the relationship of one feature relative to another feature as shown in the drawings. It is understood that, depending on the placement of the product, the terms of spatial relative positions may be intended to include different orientations other than those shown in the drawings, and should not be construed as limitations on the claims. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural schematic diagram of embodiment 1 of the utility model.

[0022] Figure 2 yes Figure 1 Schematic diagram of the structure of the middle dish body and the mesh screen.

[0023] Figure 3 yes Figure 2 Exploded diagram.

[0024] Figure 4 yes Figure 2 Another structural explosion diagram. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] like Figure 1 The high-efficiency oocyte collection dish shown in the figure comprises a dish body 1 and a dish cover 2 covering the dish body 1, and a detachable mesh screen 3 for enriching oocytes is also installed inside the dish body 1, and the mesh screen 3 is in the shape of an inverted truncated cone with an open top surface, and the diameter of the upper bottom surface 31 of the mesh screen is 50 mm, and the diameter of the lower bottom surface 32 of the mesh screen is 30 mm. The side surface 33 of the mesh screen is provided with a liquid surface through hole 34 for allowing a part of the follicular fluid containing blood cells to flow out, and the diameter of the liquid surface through hole 34 is 30 μm, and the distance between the center of the liquid surface through hole 34 and the lower bottom surface 32 of the mesh screen is 4 mm.

[0027] The invention concept of the utility model: the inventor found in the actual work process that there is no special dish for collecting oocytes in the field of assisted reproduction, and all ordinary culture dishes of different models are used. It is well known that follicular fluid is mainly composed of granulosa cells (including oocytes), and also contains a small amount of blood cells, hormones, cytokines and other substances. When the follicular fluid is poured into an ordinary culture dish, with the effect of inertia or tension, most of the contents (including oocytes) will be distributed in the outermost edge area of ​​the dish. Therefore, during the microscopic observation process, the operator needs to adjust the best observation position by manually moving the ordinary culture dish continuously, which will greatly increase the operation time, and will also cause the oocytes to be exposed in vitro for too long, and the in vitro exposure time is too long and will have an adverse effect on the oocytes. The inventor also tried to reduce the culture dish, and the size of the culture dish is basically the same as the microscope eyepiece, so that the oocytes can be gathered under the eyepiece, avoiding the operator to manually move the ordinary culture dish continuously to adjust the best observation position. However, as mentioned above, the follicular fluid is a complex, and the thickness of the follicular fluid and the blood cells in the follicular fluid will affect the observation of the oocyte. Therefore, simply adjusting the size of the culture dish cannot control the page height of the follicular fluid, and at the same time reduce the influence of the blood cells and other substances in the follicular fluid on the detection. Therefore, the inventors thought of adding a mesh screen 3 inside the culture dish, and controlling the diameter of the liquid surface through hole 34 to allow a part of the follicular fluid containing blood cells to flow out, while the oocyte cannot flow out, thereby enriching the oocyte, and controlling the height of the detection liquid level by controlling the distance between the center of the liquid surface through hole 34 and the bottom surface 32 of the mesh screen, and controlling the diameter of the bottom surface 32 of the mesh screen to be basically the same as the eyepiece of the microscope, thereby achieving accurate and rapid detection of the oocyte and reducing the influence of in vitro exposure on the oocyte.

[0028] In this embodiment, the liquid surface through holes 34 are evenly distributed in a circle along the central axis of the mesh screen 3 .

[0029] In this embodiment, a circle of accelerating through holes 35 evenly distributed along the central axis of the mesh 3 is further provided above the liquid level through hole 34, and the distance between the center of the accelerating through hole 35 and the lower bottom surface 32 of the mesh 3 is 10 mm. The main purpose of the accelerating through hole 35 is to accelerate the outflow speed of the follicular fluid, and the main purpose of the liquid level through hole 34 is to control the liquid level of the follicular fluid after the oocyte is enriched, and the liquid level is the optimal detection liquid level.

[0030] In this embodiment, the upper bottom surface 31 of the mesh screen extends outward to have an outer edge 36 for easy gripping.

[0031] In this embodiment, the diameter of the dish body 1 is 80 mm. The space between the dish body 1 and the mesh screen 3 is a storage area for waste fluid (follicular fluid containing blood cells).

[0032] In this embodiment, the bottom 11 of the dish body 1 is provided with four arc protrusions 12 along the central axis of the dish body 1, and the lower end of the side surface 33 of the mesh screen is clamped in the arc protrusion 12. The main function of the arc protrusion 12 is to clamp the mesh screen 3 to prevent the mesh screen 3 from shaking in the dish body 1. The mesh screen bottom surface 32 of the mesh screen 3 is open.

[0033] In this embodiment, the mesh screen 3 is made of PE.

[0034] In this embodiment, the material of the dish body 1 is glass.

[0035] The above is a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements and modifications without departing from the principle of the present invention. These improvements and modifications are also considered to be within the protection scope of the present invention.

Claims

1. An efficient oocyte collection dish, comprising a dish body and a dish cover covering the dish body, characterized in that: A detachable mesh screen for enriching oocytes is also installed inside the dish body. The mesh screen is in the shape of an inverted truncated cone with an opening on the top surface. The diameter of the upper bottom surface of the mesh screen is 50-60mm, and the diameter of the lower bottom surface of the mesh screen is 30-35mm. A liquid surface through hole is provided on the side of the mesh screen for allowing a part of the follicular fluid containing blood cells to flow out. The diameter of the liquid surface through hole is 20-80μm, and the distance between the center of the liquid surface through hole and the lower bottom surface of the mesh screen is 3-5mm.

2. The efficient oocyte collection dish according to claim 1, characterized in that: The liquid surface through holes are evenly distributed in a circle along the central axis of the mesh screen.

3. The efficient oocyte collection dish according to claim 2, characterized in that: A circle of accelerating through holes evenly distributed along the central axis of the mesh screen is also arranged above the liquid surface through hole, and the distance between the center of the accelerating through hole and the bottom surface of the mesh screen is 8-10 mm.

4. The efficient oocyte collection dish according to claim 1, characterized in that: The upper bottom surface of the mesh screen extends outwards to have an outer edge that is convenient for hand holding.

5. The efficient oocyte collection dish according to claim 1, characterized in that: The diameter of the dish body is 80-90 mm.

6. The efficient oocyte collection dish according to claim 1, characterized in that: The bottom of the dish body is provided with four arc protrusions with the central axis of the dish body in an upward direction, and the lower end of the side surface of the mesh screen is clamped in the arc protrusions.

7. The efficient oocyte collection dish according to claim 1, characterized in that: The mesh material is at least one of PE, PP, PET, PS, SB, SBC, PVA and medical nylon mesh.

8. The efficient oocyte collection dish according to claim 1, characterized in that: The material of the dish body is glass or plastic.

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