External film cryopreservation bag for cell cryopreservation tube

Abstract

The utility model relates to the related field of cell cryopreservation, in particular to an external film cryopreservation bag for a cell cryopreservation tube, which comprises a cryopreservation tube protection mechanism, and the cryopreservation tube protection mechanism comprises a cryopreservation bag, a storage cavity and a sealing zipper. After it is confirmed that the cryopreservation tube is correctly placed, the sealing zipper is zipped up to seal the inner side of the storage cavity, the cryopreservation tube is not in direct contact with liquid nitrogen under the protection of the cryopreservation bag, pollution is avoided, the cell survival rate is increased, then an air pump is used for being connected with a one-way valve to evacuate air in the storage cavity, the possibility that the cryopreservation tube is polluted in the storage process is reduced, and the safety of the cryopreservation tube is improved. According to the cryopreservation tube storage device, the cryopreservation tube is arranged in the storage cavity, no gas is arranged in the storage cavity for heat preservation, the cryopreservation tube in the storage cavity can be better frozen and stored, in addition, the storage cavity can be compressed in a negative pressure state, the cryopreservation tube can be fixed, and the situation that the cryopreservation tube shakes in the storage cavity, and the survival rate of cells is reduced is prevented.

Description

Technical Field

[0001] The utility model relates to the field related to cell freezing technology, in particular to a film freezing bag for external use of a cell freezing tube. Background Art

[0002] The main function of cryogenic tubes is to transport and store biological materials at low temperatures. They can be used for cell cryopreservation to ensure that cells still have good activity and proliferation ability after long-term storage.

[0003] Existing patent application number: CN202122394788.7 A cryogenic tube protection assembly for cell preservation, comprising a cryogenic tube, at least one cryogenic box for holding the cryogenic tube, and a liquid nitrogen preservation container for storing the cryogenic box. The cryogenic tube protection assembly uses a cryogenic box to hold the cryogenic tube, ensuring uniform contact between the liquid nitrogen and the cryogenic tube, uniform freezing temperature, and reducing the occurrence of ice blockage in the cryogenic tube. The cryogenic boxes can be stacked on each other. When storing or removing the cryogenic tube, there is no need to completely take out all the cryogenic boxes each time. It is convenient to use and has a good freezing effect.

[0004] The above patent records the technical problem that the cryopreservation tube has low external protection. When cryopreserved in low-temperature liquid nitrogen, there is a chance that liquid nitrogen will enter the cryopreservation tube and cause damage to the tube body, affecting the success rate of cell recovery in the tube. For this reason, we propose a thin film cryopreservation bag for cell cryopreservation tubes to solve the above problem. Utility Model Content

[0005] The utility model provides a thin film freezing bag for external use of a cell freezing tube, so as to solve the technical problems raised in the above-mentioned background technology.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a thin film cryopreservation bag for external use of cell cryopreservation tubes, including a cryopreservation tube protection mechanism, the cryopreservation tube protection mechanism includes a cryopreservation bag, a storage cavity and a sealing zipper, a storage cavity is opened on the inner side of the cryopreservation bag, the storage cavity is used to store cryopreservation tubes, and a sealing zipper is installed on the top opening of the storage cavity.

[0007] Preferably, the freezing bag is made of polyethylene.

[0008] Preferably, a one-way valve is installed at the bottom of the freezing bag, and the one-way valve is connected to the inner side of the storage cavity.

[0009] Preferably, a one-way valve is installed at the bottom of the freezing bag, and the one-way valve is connected to the inner side of the storage cavity.

[0010] Preferably, the cryotube protection mechanism also includes a dent and a steel clamp. The upper surface of the cryobag is horizontally provided with a dent, and the steel clamp can be clamped to the upper part of the cryobag along the dent, so that the steel clamp clamps the upper part of the storage cavity closed.

[0011] Preferably, a resin gasket is provided on the inner side wall of the steel clip.

[0012] Beneficial effects of the utility model:

[0013] The utility model is provided with a cryopreservation tube protection mechanism, which is performed by pulling open the sealing zipper horizontally, and then placing the cryopreservation tube into the inner side of the storage cavity, and after confirming that the cryopreservation tube is correctly placed, the sealing zipper is closed to seal the inner side of the storage cavity, so that the cryopreservation tube is not in direct contact with liquid nitrogen under the protection of the cryopreservation bag, thereby avoiding contamination and improving the cell survival rate, and then, using an air pump to connect the one-way valve to evacuate the gas inside the storage cavity, thereby reducing the possibility of contamination of the cryopreservation tube during storage, and there is no gas inside the storage cavity for heat preservation, so that the cryopreservation tube in the storage cavity can be better frozen and stored, and in addition, the storage cavity will also be compressed under negative pressure, which can fix the cryopreservation tube and prevent the cryopreservation tube from shaking in the storage cavity and reducing the cell survival rate.

[0014] The utility model provides a steel clip, and clamps the steel clip to the upper part of the freezing bag along the indentation, so that the steel clip clamps the upper part of the storage cavity and closes it. The steel clip is used in conjunction with a sealing zipper to greatly improve the airtightness of the storage cavity, preventing liquid nitrogen from entering the storage cavity from the opening and contaminating the freezing tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a front view structural schematic diagram of the freezing bag in the utility model;

[0016] Figure 2 This utility model Figure 1 Schematic diagram of the structure after the middle steel clamp is removed;

[0017] Figure 3 It is a left-side structural schematic diagram of the steel clamp in the utility model.

[0018] Including: cryotube protection mechanism-1, cryobag-11, storage cavity-12, sealing zipper-13, one-way valve-14, dent-15, steel clip-16, resin gasket-161. DETAILED DESCRIPTION

[0019] In order to further explain the technical solution of the present utility model, it is described in detail through specific embodiments below.

[0020] like Figure 1 As shown, the utility model provides a thin film cryopreservation bag for external use of cell cryopreservation tubes, including a cryopreservation tube protection mechanism 1, the cryopreservation tube protection mechanism 1 includes a cryopreservation bag 11, a storage cavity 12 and a sealing zipper 13, the storage cavity 12 is opened on the inner side of the cryopreservation bag 11, the storage cavity 12 is used to store cryopreservation tubes, and the top opening of the storage cavity 12 is installed with a sealing zipper 13, and the storage cavity 12 is opened and closed by the sealing zipper 13.

[0021] It is further explained that the cryopreservation bag 11 is made of polyethylene, which has excellent low-temperature resistance and can remain stable and intact at extremely low temperatures, ensuring that the cryopreservation bag 11 will not rupture in an extremely low temperature environment, thereby providing good external protection for the cryopreservation tube.

[0022] To further explain, a one-way valve 14 is installed at the bottom of the cryopreservation bag 11, and the one-way valve 14 is connected to the inner side of the storage chamber 12. The gas inside the storage chamber 12 can be evacuated through the one-way valve 14 to reduce the possibility of contamination of the cryopreservation tubes during storage. In addition, there is no gas inside the storage chamber 12 for insulation, so that the cryopreservation tubes in the storage chamber 12 can be better frozen and stored. In addition, the storage chamber 12 will also be compressed under negative pressure, which can fix the cryopreservation tubes and prevent them from shaking in the storage chamber 12 and reducing the survival rate of cells.

[0023] like Figure 2 As shown, the cryotube protection mechanism 1 in the utility model further includes a dent 15 and a steel clamp 16. The dent 15 is horizontally arranged on the upper surface of the cryobag 11. The steel clamp 16 can be clamped to the upper part of the cryobag 11 along the dent 15, so that the steel clamp 16 clamps the upper part of the storage cavity 12 tightly and closes it. The steel clamp 16 is used in conjunction with the sealing zipper 13 to greatly improve the airtightness of the storage cavity 12, preventing liquid nitrogen from entering the storage cavity 12 from the opening and contaminating the cryotube.

[0024] like Figure 3 As shown, in the utility model, a resin gasket 161 is provided on the inner wall of the steel clip 16, and the resin gasket 161 can improve the clamping effect of the steel clip 16. At the same time, the resin gasket 161 plays a role of flexible contact between the steel clip 16 and the cryopreservation bag 11, thereby preventing the steel clip 16 from clamping the cryopreservation bag 11 and causing it to rupture accidentally.

[0025] Here’s how it works:

[0026] In order to solve the technical problem that the external protection of the cryotube is low and it is easy to be contaminated during low-temperature storage, a cryotube protection mechanism 1 is set up. When in use, the sealing zipper 13 is first opened horizontally, and then the cryotube is placed inside the storage cavity 12. After confirming that the cryotube is correctly placed, the sealing zipper 13 is closed to seal the inside of the storage cavity 12, so that the cryotube is not in direct contact with liquid nitrogen under the protection of the cryopreservation bag 11, thereby avoiding contamination and improving the cell survival rate. Subsequently, a vacuum pump is used to connect the one-way valve 14 to evacuate the gas inside the storage cavity 12 to reduce the possibility of contamination of the cryotube during storage. Moreover, there is no gas inside the storage cavity 12 for insulation, so that the cryotube in the storage cavity 12 can be better frozen and stored. In addition, the storage cavity 12 will also be compressed under negative pressure, which can fix the cryotube and prevent the cryotube from shaking in the storage cavity 12 and reducing the cell survival rate.

[0027] After the storage cavity 12 is vacuum treated, the steel clip 16 is clamped to the upper part of the cryopreservation bag 11 along the indentation 15, so that the steel clip 16 clamps the upper part of the storage cavity 12 closed. The steel clip 16 is used in conjunction with the sealing zipper 13 to greatly improve the airtightness of the storage cavity 12 and prevent liquid nitrogen from entering the storage cavity 12 from the opening and contaminating the cryopreservation tube.

[0028] The utility model provides a thin film freezing bag for cell cryopreservation tubes. The freezing tube protection mechanism 1 is provided. The sealing zipper 13 is pulled open horizontally, and then the freezing tube is placed inside the storage cavity 12. After confirming that the freezing tube is correctly placed, the sealing zipper 13 is pulled up to seal the inside of the storage cavity 12, so that the freezing tube is not in direct contact with liquid nitrogen under the protection of the freezing bag 11, thereby avoiding contamination and improving the cell survival rate. Subsequently, an air pump is used to connect the one-way valve 14 to evacuate the gas inside the storage cavity 12, thereby reducing the possibility of contamination of the freezing tube during storage, and there is no liquid nitrogen inside the storage cavity 12. There is gas for heat preservation, so that the cryotubes in the storage chamber 12 can be better frozen and stored. In addition, the storage chamber 12 will also be compressed under negative pressure, which can fix the cryotubes and prevent them from shaking in the storage chamber 12 and reducing the survival rate of cells. A steel clamp 16 is provided, and the steel clamp 16 is clamped to the upper part of the cryopreservation bag 11 along the indentation 15, so that the steel clamp 16 clamps the upper part of the storage chamber 12 closed. The steel clamp 16 is used in conjunction with the sealing zipper 13 to greatly improve the airtightness of the storage chamber 12 and prevent liquid nitrogen from entering the storage chamber 12 from the opening and contaminating the cryotubes.

[0029] The above description is only a preferred example of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A film cryopreservation bag for external use of a cell cryopreservation tube, characterized in that: The invention comprises a cryotube protection mechanism (1), wherein the cryotube protection mechanism (1) comprises a cryobag (11), a storage cavity (12) and a sealing zipper (13); a storage cavity (12) is provided inside the cryobag (11); the storage cavity (12) is used to store cryotubes; and a sealing zipper (13) is installed at the top opening of the storage cavity (12).

2. The film cryopreservation bag for external use of a cell cryopreservation tube according to claim 1, characterized in that: The freezing bag (11) is made of polyethylene.

3. The film cryopreservation bag for external use of a cell cryopreservation tube according to claim 1, characterized in that: A one-way valve (14) is installed at the bottom of the freezing bag (11), and the one-way valve (14) is connected to the inner side of the storage chamber (12).

4. The film cryopreservation bag for external use of a cell cryopreservation tube according to claim 1, characterized in that: The cryotube protection mechanism (1) further comprises a notch (15) and a steel clamp (16); the notch (15) is horizontally arranged on the upper surface of the cryobag (11); the steel clamp (16) can be clamped to the upper part of the cryobag (11) along the notch (15), so that the steel clamp (16) clamps the upper part of the storage chamber (12) closed.

5. A film cryopreservation bag for external use of a cell cryopreservation tube according to claim 4, characterized in that: The inner side wall of the steel clip (16) is provided with a resin gasket (161).

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