Nonprogrammed cell frozen stock solution free of proteins

Abstract

The invention discloses a nonprogrammed cell frozen stock solution free of proteins, comprising 1 to 23 w / v% of cell membrane protective agent, 1.0 to 16w / v% of permeable cell membrane internal protective agent, 3.0 to 28w / v% of cell settlement stabilizer, and the balance pH buffer solution. The cell frozen stock solution of the invention consists of pure chemical materials without the proteins, and has stable and controllable components, long quality guarantee period, good stability of different batches, good protection effect of the cell, high cell recovery rate after the frozen stock, no influence on the cell characteristics, and no influence on the normal growth and differentiation of the cell. The cell frozen stock solution of the invention can be directly used in the cell stock without the dilution and the self preparation. The process of frozen stock is simple, a user only needs to put the cell for frozen stock into the cell frozen stock and to put the cell frozen stock in the environment of -80 DEG C directly for frozen stock without the need of programmed temperature reduction, thereby greatly enhancing the efficiency of the cell frozen stock with convenient operation.

Description

technical field

[0001] The invention relates to a cell cryopreservation solution, in particular to a protein-free cell cryopreservation solution. Background technique

[0002] Cells, especially high-value cells such as stem cells, have potential medical value and other values, and cell preservation technology is the basis for realizing these values.

[0003] Commonly used cell preservation methods include culture preservation and cryopreservation. Cultivation and preservation are time-consuming and labor-intensive, and the procedures are cumbersome. During the cultivation process, especially during long-term subculture, cells are prone to mutation, cell characteristics are lost, and preservation value is lost. Therefore, culture preservation is generally applied to cells that are easy to culture and not easy to mutate, such as some tumor cells.

[0004] Cell cryopreservation is to store cells in a low temperature environment, so that cells can temporarily escape from the g...

Examples

Embodiment 1

[0036] The composition of the cell freezing solution is as follows:

[0037] Cell membrane protective agent 1%, composed of 0.5% sucrose, 0.5% high molecular weight anhydride-500, DMSO 8%,

[0038] Cell sedimentation stabilizer 25%, composed of methylcellulose 4000 CP 15%, hydroxyethyl starch 10%,

[0039] Antioxidant 0.2%, composed of Vitamin C 0.1%, Glutathione 0.1%,

[0040] Cell nutrient 1.0%, composed of glutamine 0.4%, sodium pyruvate 0.6%,

[0041] PBS buffer, balance.

Embodiment 2

[0043] The composition of the cell freezing solution is as follows:

[0044] Cell membrane protective agent 23%, composed of 11% trehalose, 5% panose, 7% middle molecular anhydride-70,

[0045] Permeable cell membrane inner protective agent 5%, composed of 2% propylene glycol and 3% glycerol,

[0046] Cell sedimentation stabilizer 8%, composed of 5% soluble starch, 3% dextrin,

[0047] Glutathione 0.3%,

[0048] Glutamine 0.3%

[0049] PBS buffer, balance.

Embodiment 3

[0051] The composition of the cell freezing solution is as follows:

[0052] Cell membrane protective agent 5%, composed of trehalose 2%, xylose 3%,

[0053] Permeable cell membrane inner protective agent 13%, composed of DMSO 5%, propylene glycol 8%,

[0054] Cell sedimentation stabilizer 20%, composed of methylcellulose 1500 CP 5%, hydroxyethyl starch 15%,

[0055] Antioxidant 0.1%, consisting of Vitamin C,

[0056] Cell Nutrition 0.5%, consisting of Sodium Pyruvate,

[0057] pH buffer, balance.

technical field

[0001] The invention relates to a cell cryopreservation solution, in particular to a protein-free cell cryopreservation solution. Background technique

[0002] Cells, especially high-value cells such as stem cells, have potential medical value and other values, and cell preservation technology is the basis for realizing these values.

[0003] Commonly used cell preservation methods include culture preservation and cryopreservation. Cultivation and preservation are time-consuming and labor-intensive, and the procedures are cumbersome. During the cultivation process, especially during long-term subculture, cells are prone to mutation, cell characteristics are lost, and preservation value is lost. Therefore, culture preservation is generally applied to cells that are easy to culture and not easy to mutate, such as some tumor cells.

[0004] Cell cryopreservation is to store cells in a low temperature environment, so that cells can temporarily escape from the g...

Examples

Embodiment 1

[0036] The composition of the cell freezing solution is as follows:

[0037] Cell membrane protective agent 1%, composed of 0.5% sucrose, 0.5% high molecular weight anhydride-500, DMSO 8%,

[0038] Cell sedimentation stabilizer 25%, composed of methylcellulose 4000 CP 15%, hydroxyethyl starch 10%,

[0039] Antioxidant 0.2%, composed of Vitamin C 0.1%, Glutathione 0.1%,

[0040] Cell nutrient 1.0%, composed of glutamine 0.4%, sodium pyruvate 0.6%,

[0041] PBS buffer, balance.

Embodiment 2

[0043] The composition of the cell freezing solution is as follows:

[0044] Cell membrane protective agent 23%, composed of 11% trehalose, 5% panose, 7% middle molecular anhydride-70,

[0045] Permeable cell membrane inner protective agent 5%, composed of 2% propylene glycol and 3% glycerol,

[0046] Cell sedimentation stabilizer 8%, composed of 5% soluble starch, 3% dextrin,

[0047] Glutathione 0.3%,

[0048] Glutamine 0.3%

[0049] PBS buffer, balance.

Embodiment 3

[0051] The composition of the cell freezing solution is as follows:

[0052] Cell membrane protective agent 5%, composed of trehalose 2%, xylose 3%,

[0053] Permeable cell membrane inner protective agent 13%, composed of DMSO 5%, propylene glycol 8%,

[0054] Cell sedimentation stabilizer 20%, composed of methylcellulose 1500 CP 5%, hydroxyethyl starch 15%,

[0055] Antioxidant 0.1%, consisting of Vitamin C,

[0056] Cell Nutrition 0.5%, consisting of Sodium Pyruvate,

[0057] pH buffer, balance.

Images