Preparation method of human homologous tendon

A tendon and human technology, applied in medical science, prosthesis, etc., can solve the problems of complex operation steps, large changes in the original structure and strength of tendon, and many drug residues, so as to maintain strength, high-efficiency killing effect, and maintain mechanics. performance effect

CN102166373BInactive Publication Date: 2013-05-22山西奥瑞生物材料有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2013-05-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method of human homologous tendon, which belongs to the technical field of biomedical materials. The invention mainly solves the technical problems of immunological rejection, bacterial infection, virus infection, complicated operation steps, and the like in the traditional method for transplanting homologous tendon. The preparation method of human homologous tendon comprises the following steps of: (1) cleaning the human homologous tendon; (2) freezing the cleaned human homologous tendon for 30-90 days; (3) trimming loose connective tissues surrounding the tendon; (4) immersing the trimmed tendon material in a mixed solution of peroxyacetic acid, ethanol and water, standing still for inactivation of viruses; (5) cleaning the inactivated tendon with water until the content of the peroxyacetic acid in the cleaning solution reaches a certain value for the last time; (6) trimming the tendon again, dripping the water on the surface of the tendon, vacuumizing the trimmed tendon under an aseptic condition, and packing twice by common packaging; and (7) radiating the packed tendon by using Cobalt-60 for disinfection again. The invention has the advantages of no immunological rejection, no bacterial or virus infection, easiness for operation, no drug residue, and the like.
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Description

technical field

[0001] The invention relates to a preparation method of human allogeneic tendon, in particular to a preparation method which can be used for making allogeneic tendon, and belongs to the technical field of medical biomaterials. Background technique

[0002] Tendon is a regularly arranged dense connective tissue composed of collagen, tenocytes and tendon matrix. Collagen is the main component of tendon, accounting for 70-90% of the dry weight of tendon, and plays a leading role in maintaining the strength of tendon. Tenocytes are a kind of fibroblasts with a special shape and a small number, which are the main carriers of antigens in allogeneic tendon transplantation. The tendon matrix provides the structural support for the collagen tissue, and its elasticity also helps reduce friction and shear forces acting on the tendon. Tendons connect skeletal muscle to muscle tissue, attach the muscle tissue to bone or fascia, transmit force to the skeletal system, and...

Examples

Embodiment 1

[0059] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:

[0060] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;

[0061] 2) Store the cleaned tendon material in a freezer at -60°C for 30 days;

[0062] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 30 minutes, and cut off the loose connective tissue around the tendon;

[0063] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 0.05:10:70, and let it stand for 20 minutes to inactivate the virus;

[0064] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 0.1 ppm;

[0065] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice ...

Embodiment 2

[0068] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:

[0069] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;

[0070] 2) Store the cleaned tendon material at -70°C for 60 days;

[0071] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 45 minutes, and cut off the loose connective tissue around the tendon;

[0072] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 1:15:80, and let it stand for 35 minutes to inactivate the virus;

[0073] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 3 ppm;

[0074] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice again;

[0075] 7...

Embodiment 3

[0077] A kind of human allogenic tendon preparation method in the present embodiment comprises the following steps:

[0078] 1) extract the human donor tendon material and clean the blood stains on the surface of the tendon with saline;

[0079] 2) Store the cleaned tendon material at -80°C for 90 days;

[0080] 3) Take the tendon material frozen in step (2), rewarm it at room temperature for 60 minutes, and cut off the loose connective tissue around the tendon;

[0081] 4) Immerse the trimmed tendon material in a mixed solution of peracetic acid, ethanol and water with a weight ratio of 2:30:90, and let it stand for 50 minutes to inactivate the virus;

[0082] 5) Wash the inactivated tendon with water until the peracetic acid content in the last cleaning solution is 5 ppm;

[0083] 6) Trim the tendon cleaned in step (5) again, drain the water on the surface of the tendon, vacuum pack and trim the tendon under aseptic conditions, and pack it normally twice again;

[0084] 7...