Method for preparing blood fat reducing peptide by use of silkworm chrysalis

Abstract

The invention discloses a method for preparing blood fat reducing peptide by use of silkworm chrysalis, which comprises the following steps: 1) mixing the degreased dry silkworm chrysalis powder or silkworm chrysalis protein powder with water, regulating the pH value to 6.8-7.2, heating to 85-95 DEG C, preserving heat for 8-12 minutes and naturally cooling to 45-50 DEG C; adding papain for an enzymolysis reaction; heating the product of the enzymolysis reaction to 95-105 DEG C and keeping the temperature for 10-15 minutes; naturally cooling to room temperature to terminate the enzymolysis reaction; and centrifuging to obtain enzymolysis liquid; 2) performing decoloration treatment on the enzymolysis liquid at 42-48 DEG C by use of active carbon, and filtering the enzymolysis liquid after the decoloration; and 3) performing ultrafiltration treatment on the rough-filtration enzymolysis liquid under a working pressure of 0.1-0.2MPa and a working temperature of 0-45 DEG C by use of an ultrafiltration membrane to obtain ultrafiltration enzymolysis liquid; and sequentially performing vacuum concentration and freeze drying on the ultrafiltration enzymolysis liquid to obtain the blood fat reducing peptide.

Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing blood lipid-lowering peptides by using silkworm chrysalis. Background technique

[0002] HMG-CoA reductase is the key enzyme that catalyzes the generation of dimethylvaleric acid (MVA) from 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA), which is the limit of cholesterol synthesis in vivo. It is also the target of the most important clinical drug for hyperlipidemia at present. HMG-CoA reductase inhibitors are one of the main means of blood lipid-lowering functional ingredients and drug screening. At present, most of the lipid-lowering drugs used in the market are chemically synthesized statins. Although they have a good effect on improving cardiovascular and cerebrovascular diseases caused by hyperlipidemia, long-term use will bring a series of adverse reactions and side effect.

[0003] Extracting functional peptides with nutritiona...

Examples

Embodiment 1

[0031] Embodiment 1, a kind of method utilizing silkworm chrysalis to prepare hypolipidemic peptide, carries out following steps successively:

[0032] 1) Weigh 50kg of pulverized degreased dry silkworm chrysalis powder (moisture content 1.5%, protein content 75.03%, raw material comes from Nantong Fulier Biological Products Co., Ltd.), add 500kg water and stir well, adjust the pH value to 7.0 (use The concentration is 2mol / L HCl solution, NaOH solution to adjust, this adjustment method belongs to the conventional technology), first raise the temperature to 90°C within 5~10min and keep this temperature for 10min, then naturally cool to 45°C~50°C and keep it warm, as the starting point of the reaction starting material; add 1 kg of papain (enzyme activity: 800AU / g) to the reaction starting material, continue to incubate and stir continuously for enzymolysis reaction for 3 hours.

[0033] After the time is up, heat the product obtained from the enzymatic hydrolysis reaction to 1...

Embodiment 2

[0039] Embodiment 2, a kind of method utilizing silkworm chrysalis to prepare hypolipidemic peptide, carries out the following steps successively:

[0040] 1) Weigh 2kg of silkworm chrysalis protein powder, add 20kg of water and stir well, adjust the pH value to 7.0, heat up to 90°C within 5~10min and keep this temperature for 10min, then cool naturally to 45°C~50°C to keep warm, As a reaction starter; add 40 g of papain (enzyme activity: 800 AU / g) to the reaction starter, continue to incubate and stir continuously for enzymolysis reaction for 3 hours. After the time is up, heat the enzymolysis reaction solution to 100°C within 5-10 minutes, and keep it warm for 10 minutes; then cool it down to room temperature naturally to terminate the reaction;

[0041] Next, centrifuge in a J-6M large-capacity refrigerated centrifuge (BECKMAN company) at 3000r / min at 4°C for 20min to obtain a supernatant of 13.5kg, which is the enzymatic hydrolysis solution; the precipitate obtained by cen...

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing blood lipid-lowering peptides by using silkworm chrysalis. Background technique

[0002] HMG-CoA reductase is the key enzyme that catalyzes the generation of dimethylvaleric acid (MVA) from 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA), which is the limit of cholesterol synthesis in vivo. It is also the target of the most important clinical drug for hyperlipidemia at present. HMG-CoA reductase inhibitors are one of the main means of blood lipid-lowering functional ingredients and drug screening. At present, most of the lipid-lowering drugs used in the market are chemically synthesized statins. Although they have a good effect on improving cardiovascular and cerebrovascular diseases caused by hyperlipidemia, long-term use will bring a series of adverse reactions and side effect.

[0003] Extracting functional peptides with nutritiona...

Examples

Embodiment 1

[0031] Embodiment 1, a kind of method utilizing silkworm chrysalis to prepare hypolipidemic peptide, carries out following steps successively:

[0032] 1) Weigh 50kg of pulverized degreased dry silkworm chrysalis powder (moisture content 1.5%, protein content 75.03%, raw material comes from Nantong Fulier Biological Products Co., Ltd.), add 500kg water and stir well, adjust the pH value to 7.0 (use The concentration is 2mol / L HCl solution, NaOH solution to adjust, this adjustment method belongs to the conventional technology), first raise the temperature to 90°C within 5~10min and keep this temperature for 10min, then naturally cool to 45°C~50°C and keep it warm, as the starting point of the reaction starting material; add 1 kg of papain (enzyme activity: 800AU / g) to the reaction starting material, continue to incubate and stir continuously for enzymolysis reaction for 3 hours.

[0033] After the time is up, heat the product obtained from the enzymatic hydrolysis reaction to 1...

Embodiment 2

[0039] Embodiment 2, a kind of method utilizing silkworm chrysalis to prepare hypolipidemic peptide, carries out the following steps successively:

[0040] 1) Weigh 2kg of silkworm chrysalis protein powder, add 20kg of water and stir well, adjust the pH value to 7.0, heat up to 90°C within 5~10min and keep this temperature for 10min, then cool naturally to 45°C~50°C to keep warm, As a reaction starter; add 40 g of papain (enzyme activity: 800 AU / g) to the reaction starter, continue to incubate and stir continuously for enzymolysis reaction for 3 hours. After the time is up, heat the enzymolysis reaction solution to 100°C within 5-10 minutes, and keep it warm for 10 minutes; then cool it down to room temperature naturally to terminate the reaction;

[0041] Next, centrifuge in a J-6M large-capacity refrigerated centrifuge (BECKMAN company) at 3000r / min at 4°C for 20min to obtain a supernatant of 13.5kg, which is the enzymatic hydrolysis solution; the precipitate obtained by cen...