Tissue culture and rapid propagation method of millettiadielsiana harms

A technology of rapid propagation of twig and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of maintaining hereditary traits, high multiplication coefficient, and shortening the cultivation period

CN104604691AInactive Publication Date: 2015-05-13湖南省农业生物资源利用研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2015-05-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a tissue culture and rapid propagation method of millettiadielsiana harms. The method comprises the following steps: selecting and treating of an explant, primary culture, differential culture, multiplication culture and rooting culture, thus forming a complete set of millettiadielsiana harms tissue culture and rapid propagation technology. The tissue culture and rapid propagation method has the characteristics of being short in culture period, high in multiplication coefficient, fast in multiplication speed, good in rooting effect, maintaining the excellent hereditary character of mother plant and the like, the factorized rapid seedling of the millettiadielsiana harms can be achieved, the multiplication coefficient can achieve 15.0-18.0, and compared with the tissue culture and rapid propagation of sterile seedlings obtained by taking seed sprouts, single buds and stems with buds as materials, the tissue culture and rapid propagation method is good in multiplication effect, high in multiplication efficiency, the stages of sprouting and growing of seeds into sterile seedlings in the culture media can be reduced, so that the culture period is shortened, thus providing a tissue culture and rapid propagation production technology for the medicinal and appreciable dual-use excellent material.
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Description

technical field

[0001] The invention belongs to the technical field of tissue culture of japonica japonica, and in particular relates to a method for tissue culture and rapid propagation of japonica japonica. Background technique

[0002] Millettia dielsiana Harms (Millettia dielsiana Harms), also known as Millettia dielsiana Harms, is a perennial semi-evergreen woody vine of the family Papilionaceae Millettia, most of which are still in a wild state. Its vines and roots can be used medicinally, which can promote qi, relieve heat, stop bleeding, drive wind and dehumidify, dredge meridians and activate collaterals. The seeds can also be used as oil materials. In gardens, it can be used for greening on scaffolding, rockery, walls, slopes, ground covers, roofs, bonsai, etc. It is a good medicinal and ornamental landscaping plant. In recent years, due to the fact that the thuja vine is not only an important medicinal plant (since the 1970s, it has been widely excavated in Guang...

Examples

Embodiment 1

[0030] A method for rapid propagation of Tachibana fragrans tissue culture, comprising the following steps

[0031] (1) Explant selection and processing:

[0032] Selection of explants: Select the vigorously growing, no pests and diseases of the twig plants as the propagation material, and pick the young shoots and young leaves from the first and second year branches in the middle and late March as the explants.

[0033] The selection of the explant material is the young leaves that have just opened on the young shoots that grow vigorously, have no damage by diseases and insect pests, no mechanical damage, and no lignification.

[0034] Explant treatment: Rinse the retrieved young leaves with a small amount of washing powder and place them in a bottle, seal the mouth of the bottle with a single layer of medical gauze, rinse in tap water for 60 minutes, pour out the water in the bottle, and put the material Place it in an ultra-clean workbench, firstly treat it with 75% alcoho...