Heart simulation structure as well as forming method and special mold thereof

Abstract

The invention discloses a heart simulation structure as well as a forming method and a special mold thereof and belongs to the field of organ production, biological materials and medical instruments. The heart simulation structure comprises a main body structure, at least one heart chamber structure, at least one blood vessel system and at least one nervous system, wherein the main body structure is a mixture of hydrogel and myocardial seed cells, is high in biocompatibility and can facilitate realization of a blood pumping function of a heart; each blood vessel system is dendritically or spirally distributed on the periphery of each heart chamber structure; each nervous system is located in the main body structure. The special mold comprises at least one casing top, at least one casing bottom, at least one air sac, at least one blood vessel system catheter and at least one nervous system catheter, wherein the at least one casing top and the at least one casing bottom are sealed through embedment assembly to constitute the outside of the mold; the at least one air sac, the at least one blood vessel system catheter and the at least one nervous system catheter are located inside the special mold. A new idea is provided for in-vitro rapid construction of the artificial heart, and the heart simulation structure is similar to real hearts of animals in form and function.

Description

technical field

[0001] The invention belongs to the field of organ manufacturing, biomaterials and medical instruments, and relates to a heart simulation structure, its forming method and a special mold. Background technique

[0002] Compared with (semi) mechanical artificial organs, biological artificial organs have stronger biocompatibility and animal body coordination. Biological artificial organs also have the potential to minimize disadvantages in terms of transplant rejection. For now, scientists aim to provide autologous artificial organs to patients in dire need of organ transplants. From a macro and micro perspective, the combination of stem cell technology, biomaterials and engineering technologies can provide unlimited possibilities for the manufacture of artificial organs.

[0003] Most of the current artificial hearts use biomechanical means to partially or completely replace the pumping function of the heart to maintain blood circulation throughout the body. ...

Examples

Embodiment 1

[0047] Example 1 A method for preparing a heart simulation structure containing a ventricle and an atrium by using the combined mold method

[0048] 1) Prepare a special mold: Utilize the method of three-dimensional punching to prepare the shell top and shell bottom of a special mold containing a ventricle and an atrium (total two heart structural chambers) heart simulation structure. The material used is ABS plastic, and it is polished and profiled. Ensure that the top of the shell and the bottom of the shell can be molded together;

[0049] 2) Prepare the vascular system: mix part of the prepared sodium alginate with a mass percentage concentration of 2% and vascular endothelial cells so that the cell density is 10 6 pcs / mL; the vascular system catheter with the inner layer of polyurethane and the outer layer of sodium alginate hydrogel containing vascular endothelial cells were prepared by double-nozzle 3D printing method, and CaCl with a mass fraction of 2% was used 2 Cro...

Embodiment 2

[0054] Example 2 A method of preparing a heart simulation structure containing a single ventricle by using the combined mold method

[0055] 1) Preparing a special mold: using machining to prepare the shell top and shell bottom of a special mold containing a single-ventricular heart simulation structure, the material used is plexiglass, and polished to ensure that the shell top and shell bottom can be molded;

[0056] 2) Prepare the vascular system: mix part of the prepared sodium alginate with a mass percentage concentration of 4% and vascular smooth muscle cells, so that the cell density is 10 4 pieces / mL; the vascular system catheter with polycaprolactone as the inner layer and sodium alginate hydrogel containing vascular smooth muscle cells as the outer layer was prepared by double-nozzle 3D printing method, and CaCl with a mass fraction of 1% was used 2 Cross-linking and fixing sodium alginate to obtain a dendritic vascular system;

[0057] 3) Prepare the nervous system:...

Embodiment 3

[0062] Example 3 A method for preparing a simulated heart structure containing two ventricles and two atria by using the combined mold method

[0063] 1) Prepare a special mold: Utilize the method of three-dimensional punching to prepare the shell top and shell bottom of the special mold containing the heart simulation structure of two ventricles and two atria (four heart chamber structures). The material used is stainless steel, and it is polished and profiled to ensure Housing top and housing bottom can be molded together;

[0064] 2) Prepare the vascular system: Mix a portion of the prepared gelatin with a mass percentage concentration of 20% and 1% fibrinogen in equal volumes, and add a mixture of adipose-derived stem cells / vascular endothelial cells (1:1) to make the cell density 10 6 pieces / mL; the vascular system catheter with the inner layer of polyurethane and the outer layer of dendritic vascular system with adipose-derived stem cells / vascular endothelial cell gelati...

technical field

[0001] The invention belongs to the field of organ manufacturing, biomaterials and medical instruments, and relates to a heart simulation structure, its forming method and a special mold. Background technique

[0002] Compared with (semi) mechanical artificial organs, biological artificial organs have stronger biocompatibility and animal body coordination. Biological artificial organs also have the potential to minimize disadvantages in terms of transplant rejection. For now, scientists aim to provide autologous artificial organs to patients in dire need of organ transplants. From a macro and micro perspective, the combination of stem cell technology, biomaterials and engineering technologies can provide unlimited possibilities for the manufacture of artificial organs.

[0003] Most of the current artificial hearts use biomechanical means to partially or completely replace the pumping function of the heart to maintain blood circulation throughout the body. ...

Examples

Embodiment 1

[0047] Example 1 A method for preparing a heart simulation structure containing a ventricle and an atrium by using the combined mold method

[0048] 1) Prepare a special mold: Utilize the method of three-dimensional punching to prepare the shell top and shell bottom of a special mold containing a ventricle and an atrium (total two heart structural chambers) heart simulation structure. The material used is ABS plastic, and it is polished and profiled. Ensure that the top of the shell and the bottom of the shell can be molded together;

[0049] 2) Prepare the vascular system: mix part of the prepared sodium alginate with a mass percentage concentration of 2% and vascular endothelial cells so that the cell density is 10 6 pcs / mL; the vascular system catheter with the inner layer of polyurethane and the outer layer of sodium alginate hydrogel containing vascular endothelial cells were prepared by double-nozzle 3D printing method, and CaCl with a mass fraction of 2% was used 2 Cro...

Embodiment 2

[0054] Example 2 A method of preparing a heart simulation structure containing a single ventricle by using the combined mold method

[0055] 1) Preparing a special mold: using machining to prepare the shell top and shell bottom of a special mold containing a single-ventricular heart simulation structure, the material used is plexiglass, and polished to ensure that the shell top and shell bottom can be molded;

[0056] 2) Prepare the vascular system: mix part of the prepared sodium alginate with a mass percentage concentration of 4% and vascular smooth muscle cells, so that the cell density is 10 4 pieces / mL; the vascular system catheter with polycaprolactone as the inner layer and sodium alginate hydrogel containing vascular smooth muscle cells as the outer layer was prepared by double-nozzle 3D printing method, and CaCl with a mass fraction of 1% was used 2 Cross-linking and fixing sodium alginate to obtain a dendritic vascular system;

[0057] 3) Prepare the nervous system:...

Embodiment 3

[0062] Example 3 A method for preparing a simulated heart structure containing two ventricles and two atria by using the combined mold method

[0063] 1) Prepare a special mold: Utilize the method of three-dimensional punching to prepare the shell top and shell bottom of the special mold containing the heart simulation structure of two ventricles and two atria (four heart chamber structures). The material used is stainless steel, and it is polished and profiled to ensure Housing top and housing bottom can be molded together;

[0064] 2) Prepare the vascular system: Mix a portion of the prepared gelatin with a mass percentage concentration of 20% and 1% fibrinogen in equal volumes, and add a mixture of adipose-derived stem cells / vascular endothelial cells (1:1) to make the cell density 10 6 pieces / mL; the vascular system catheter with the inner layer of polyurethane and the outer layer of dendritic vascular system with adipose-derived stem cells / vascular endothelial cell gelati...

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