A kind of preparation method of Pleurotus ostreatus female seed medium and Pleurotus ostreatus female seed medium

Abstract

The invention relates to a method for preparing a culture medium of oyster mushroom mother species and a culture medium of oyster mushroom mother species, belonging to the technical field of culture medium preparation. The Pleurotus ostreatus female seed culture medium that the present invention makes adds the filter juice that cottonseed hulls and corn cobs boil on the basis of conventional PDA medium, adds the cellulose natural culture medium that is suitable for the growth of Pleurotus ostreatus hyphae, reduces Reducing the content of potato, glucose and agar powder in the raw materials is beneficial to reduce the metabolic activity of Pleurotus ostreatus mycelium, thereby reducing the variation speed of Pleurotus ostreatus parent species and preventing the degradation of the excellent properties of Pleurotus ostreatus. The mycelial growth speed and growth vigor of the Pleurotus ostreatus parent species transferred by using the medium are significantly better than those of the Pleurotus ostreatus cultured on the conventional PDA medium.

Description

technical field

[0001] The invention relates to a method for preparing a culture medium of oyster mushroom mother species and a culture medium of oyster mushroom mother species, belonging to the technical field of culture medium preparation. Background technique

[0002] Edible fungus strains generally denominator species, original species and cultivated species. The parent species is a strain that is directly isolated from fruiting bodies (tissues or spores) and mushroom wood (ear wood). The medium is often nutrient-rich and easily absorbed potato agar medium (PDA medium).

[0003] The mother species of Pleurotus ostreatus is generally preserved on an inclined plane. Generally, the mother species of Pleurotus ostreatus is stored in the refrigerator at 4°C. However, at this temperature, the metabolic activity of Pleurotus ostreatus hyphae has not stopped, and the metabolic activity of Pleurotus ostreatus mycelium is vigorous. The rate of mutation is also faster.

[0004] ...

Examples

Embodiment 1

[0026] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0027] 1) A. Take 30g of fresh, mildew-free cottonseed hulls and 70g of corncobs, wrap them in four layers of gauze, soak in 500mL of water for 10 hours, then boil for 30 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0028] B. Take 160g of peeled and sliced ​​potatoes, put them in 500mL of water and boil for 30min, until the potatoes are cooked but not rotten, use four layers of gauze to filter to obtain the filtrate, which is the potato juice;

[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.

[0030] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

Embodiment 2

[0032] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0033] 1) A. Take 25g of fresh, mildew-free cottonseed hulls and 75g of corn cobs, wrap them in four layers of gauze, soak in 400mL of water for 6 hours, then boil for 20 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0034] B. Take 150g of peeled and sliced ​​potatoes, put them in 400mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0035] 2) Mix potato filtered juice and cellulose filtered juice with 16 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.

[0036] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

Embodiment 3

[0038] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0039] 1) A. Take 35g of fresh, mildew-free cottonseed hulls and 65g of corn cobs, wrap them in four layers of gauze, soak in 450mL of water for 8 hours, then boil for 40 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0040] B. Take 170g of peeled and sliced ​​potatoes, put them in 450mL of water and boil for 40min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0041] 2) Mix potato filtered juice and cellulose filtered juice with 11g glucose and 17g agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45 minutes.

[0042] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

technical field

[0001] The invention relates to a method for preparing a culture medium of oyster mushroom mother species and a culture medium of oyster mushroom mother species, belonging to the technical field of culture medium preparation. Background technique

[0002] Edible fungus strains generally denominator species, original species and cultivated species. The parent species is a strain that is directly isolated from fruiting bodies (tissues or spores) and mushroom wood (ear wood). The medium is often nutrient-rich and easily absorbed potato agar medium (PDA medium).

[0003] The mother species of Pleurotus ostreatus is generally preserved on an inclined plane. Generally, the mother species of Pleurotus ostreatus is stored in the refrigerator at 4°C. However, at this temperature, the metabolic activity of Pleurotus ostreatus hyphae has not stopped, and the metabolic activity of Pleurotus ostreatus mycelium is vigorous. The rate of mutation is also faster.

[0004] ...

Examples

Embodiment 1

[0026] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0027] 1) A. Take 30g of fresh, mildew-free cottonseed hulls and 70g of corncobs, wrap them in four layers of gauze, soak in 500mL of water for 10 hours, then boil for 30 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0028] B. Take 160g of peeled and sliced ​​potatoes, put them in 500mL of water and boil for 30min, until the potatoes are cooked but not rotten, use four layers of gauze to filter to obtain the filtrate, which is the potato juice;

[0029] 2) Mix potato filtered juice and cellulose filtered juice with 10 g of glucose and 16 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 35 minutes.

[0030] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

Embodiment 2

[0032] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0033] 1) A. Take 25g of fresh, mildew-free cottonseed hulls and 75g of corn cobs, wrap them in four layers of gauze, soak in 400mL of water for 6 hours, then boil for 20 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0034] B. Take 150g of peeled and sliced ​​potatoes, put them in 400mL of water and boil for 20 minutes until the potatoes are cooked but not rotten, and filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0035] 2) Mix potato filtered juice and cellulose filtered juice with 16 g of glucose and 15 g of agar, add water to make up to 1000 mL, and sterilize by high-pressure steam at 121°C for 40 minutes.

[0036] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

Embodiment 3

[0038] In the present embodiment, the preparation method of flat mushroom mother kind culture medium comprises the steps:

[0039] 1) A. Take 35g of fresh, mildew-free cottonseed hulls and 65g of corn cobs, wrap them in four layers of gauze, soak in 450mL of water for 8 hours, then boil for 40 minutes, and filter with two layers of gauze to obtain the filtrate, which is cellulose filtered juice;

[0040] B. Take 170g of peeled and sliced ​​potatoes, put them in 450mL of water and boil for 40min until the potatoes are cooked but not rotten, filter with four layers of gauze to obtain the filtrate, which is the potato juice;

[0041] 2) Mix potato filtered juice and cellulose filtered juice with 11g glucose and 17g agar, add water to make up to 1000mL, and sterilize under high pressure steam at 121°C for 45 minutes.

[0042] The mother seed culture medium of Pleurotus ostreatus in this embodiment is prepared by the above method.

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