Composition and preparation method thereof

Abstract

The invention discloses a composition. The composition comprises a sterile injector, a first solution and a second solution are mixed in the sterile injector. An applying part of the composition is ajoint cavity. Since mesenchymal stem cells are derived from a mesoderm, the mesenchymal stem cells not only have the potential to differentiate into bone, cartilage, adipose and myoblast, but also have the potential to differentiate into neurons and the potential of convenient obtaining, rich sources, easiness in expand, low immunogenicity and multi-directional differentiation potential; hyaluronic acid exerts adjustment function through interaction with the hyaluronic acid-binding protein in the stroma and cell membrane, so as to inhibit inflammation, can also produce barriers make pain receptors to relieve pain, also regulates the local microenvironment of the joints and activates endogenous progenitor cells through the interaction of cytokines and cells, thus exerting the effect of repairing damaged tissues.

Description

technical field

[0001] The invention relates to the field of biomedical engineering, which relates to a composition of drugs for treating and / or preventing osteoarthritis, in particular to a composition and a preparation method thereof. Background technique

[0002] Osteoarthritis, the most common form of arthritis, is characterized by a slow degenerative process of articular cartilage, subchondral bone, with marginal osteophyte formation and low-grade inflammation. The chronic form of osteoarthritis is believed to affect 15% of the population. Of these, a quarter were severely disabled. The cause of most cases of osteoarthritis is unknown and is referred to as "primary osteoarthritis". When the cause of osteoarthritis is known, it is called "secondary osteoarthritis". Secondary osteoarthritis is caused by another disease or condition. Conditions that can lead to secondary osteoarthritis include repeated injury or surgery of joint structures, joint abnormalities at birth...

Examples

preparation example Construction

[0043] Such as figure 1 Shown, the present invention also provides a kind of preparation method of composition, comprises the steps:

[0044] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the mesoderm, a type of cell with multidirectional differentiation potential, which exist in human bone marrow, adipose tissue, umbilical cord of newborns, placenta and other tissues. It has the potential to differentiate into cartilage, fat and myoblasts, and also has the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiat...

Embodiment 1

[0055] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the umbilical cord of newborns. Mesenchymal stem cells not only have the potential to differentiate into bone, cartilage, fat, and myoblasts, but also have the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiation potential. The samples were stripped of Wharton's jelly in a biological safety cabinet, and in the culture bottle, 1g of Wharton's jelly was added to each bottle, and the incubator was CO 2 an incubator, and the mesenchymal stem cells are primary...

Embodiment 2

[0068] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the mesoderm, a type of cell with multidirectional differentiation potential, which exist in human bone marrow, adipose tissue, umbilical cord of newborns, placenta and other tissues. It has the potential to differentiate into cartilage, fat and myoblasts, and also has the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiation potential. The samples were stripped of Wharton's jelly in a biological safety cabinet, and in the culture bottle, 1g of Wharton's j...

technical field

[0001] The invention relates to the field of biomedical engineering, which relates to a composition of drugs for treating and / or preventing osteoarthritis, in particular to a composition and a preparation method thereof. Background technique

[0002] Osteoarthritis, the most common form of arthritis, is characterized by a slow degenerative process of articular cartilage, subchondral bone, with marginal osteophyte formation and low-grade inflammation. The chronic form of osteoarthritis is believed to affect 15% of the population. Of these, a quarter were severely disabled. The cause of most cases of osteoarthritis is unknown and is referred to as "primary osteoarthritis". When the cause of osteoarthritis is known, it is called "secondary osteoarthritis". Secondary osteoarthritis is caused by another disease or condition. Conditions that can lead to secondary osteoarthritis include repeated injury or surgery of joint structures, joint abnormalities at birth...

Examples

preparation example Construction

[0043] Such as figure 1 Shown, the present invention also provides a kind of preparation method of composition, comprises the steps:

[0044] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the mesoderm, a type of cell with multidirectional differentiation potential, which exist in human bone marrow, adipose tissue, umbilical cord of newborns, placenta and other tissues. It has the potential to differentiate into cartilage, fat and myoblasts, and also has the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiat...

Embodiment 1

[0055] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the umbilical cord of newborns. Mesenchymal stem cells not only have the potential to differentiate into bone, cartilage, fat, and myoblasts, but also have the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiation potential. The samples were stripped of Wharton's jelly in a biological safety cabinet, and in the culture bottle, 1g of Wharton's jelly was added to each bottle, and the incubator was CO 2 an incubator, and the mesenchymal stem cells are primary...

Embodiment 2

[0068] S1. Take a sample and cut it into 1mm 3 The small pieces of the tissue were planted in a T75 culture bottle, and 6ml of mesenchymal stem cell culture medium was added to each bottle, the tissue pieces were mixed, and cultured in an incubator. Change the medium after 7 days, and when the cell confluency reaches 30%, it can be subcultured. In S1, mesenchymal stem cells are derived from the mesoderm, a type of cell with multidirectional differentiation potential, which exist in human bone marrow, adipose tissue, umbilical cord of newborns, placenta and other tissues. It has the potential to differentiate into cartilage, fat and myoblasts, and also has the potential to differentiate into neurons. Because it has the advantages of convenient acquisition, abundant sources, easy expansion, low immunogenicity and multi-directional differentiation potential. The samples were stripped of Wharton's jelly in a biological safety cabinet, and in the culture bottle, 1g of Wharton's j...

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