Extraction method of polygonatum odoratum polysaccharide and polygonatum odoratum polysaccharide extractive

Abstract

The invention relates to an extraction method of polygonatum odoratum polysaccharide and a polygonatum odoratum polysaccharide extractive. The extraction method comprises the following steps of (1) enzymolysis: taking rhizoma polygonati odorati, adding water and enzyme, and performing uniform mixing and enzymatic reaction, wherein the addition quantity of the water is 6-10 times of the mass of therhizoma polygonati odorati, and the addition quantity of the enzyme is 0.2-0.4 percent of the mass of the rhizoma polygonati odorati; (2) water extraction: after enzymolysis is finished, rising a temperature, performing stirring extraction and solid-liquid separation on the rhizoma polygonati odorati to acquire an extracting solution, and performing concentration on the extracting solution; and (3) alcohol precipitation: performing mixing, standing and solid-liquid separation operation on a concentrated solution and anhydrous ethanol, and drying precipitates obtained with separation to obtainthe polygonatum odoratum polysaccharide. The extraction method has the advantages of high polysaccharide extraction rate, good purity, small energy consumption, low cost and the like; and the polysaccharide extractive has the advantages of good purity and high nutrition and health care value.

Description

technical field

[0001] The invention relates to the field of extraction of traditional Chinese medicines, in particular to a method for extracting Polygonatum polysaccharide and Polygonatum polysaccharide extract. Background technique

[0002] The traditional Chinese medicine Polygonatum Polygonatum, also known as Vitiligo, is the dry rhizome of Polygonatum modoratum (Mill.) Druce, a plant of the genus Polygonatum in the family Liliaceae. It tastes sweet and slightly bitter, and is flat and slightly warm in nature. This medicine nourishes yin and nourishes the lungs, nourishes the stomach and promotes body fluids, and has the effects of nourishing and health care, and both medicine and food. "Shen Nong's Materia Medica", "Compendium of Materia Medica" and other traditional Chinese medicine classics are listed as top grade. Modern medical research shows that Yuzhu has the functions of strengthening the heart, dilating blood vessels, lowering blood pressure, lowering blood su...

Examples

Embodiment 1

[0032] A method for extracting polygonatum polysaccharide, said method comprising the following steps:

[0033] 1) Take the Polygonatum Polygonatum raw material, grind it into coarse powder, add water and enzyme to mix evenly, keep the temperature at 40°C, and perform an enzymatic hydrolysis reaction for 2 hours, wherein the amount of water added is 8 times the mass of Polygonatum Polygonatum, and the addition of the enzyme The amount is 0.3% of Polygonatum Polygonatum mass, and the enzymes are cellulase, pectinase and hemicellulase, and the mass ratio of the cellulase, pectinase and hemicellulase is 2:1:1.

[0034] 2) The temperature of the enzymolysis solution was raised to 90° C., stirred and extracted for 1 hour, centrifuged, and the supernatant was taken.

[0035] 3) Continue to add process water with a material ratio of 1:8 to the filter cake, raise the temperature to 90°C, stir and extract for 1 hour. Centrifuge, take the supernatant, and repeat the above steps once mo...

Embodiment 2

[0039] A method for extracting polygonatum polysaccharide, said method comprising the following steps:

[0040] 1) Take the Polygonatum Polygonatum raw material, grind it into coarse powder, add water and enzyme to mix evenly, keep the temperature at 40°C, and perform an enzymatic hydrolysis reaction for 2 hours, wherein the amount of water added is 8 times the mass of Polygonatum Polygonatum, and the addition of the enzyme The amount is 0.4% of Polygonatum Polygonatum quality, and described enzyme is cellulase, pectinase, protease, and the mass ratio of described cellulase, pectinase and protease is 2:2:1.

[0041] 2) The temperature of the enzymolysis solution was raised to 90° C., stirred and extracted for 1 hour, centrifuged, and the supernatant was taken.

[0042] 3) Continue to add process water with a material ratio of 1:8 to the filter cake, raise the temperature to 90°C, stir and extract for 1 hour. Centrifuge, take the supernatant, and repeat the above steps once mo...

Embodiment 3

[0046] A method for extracting polygonatum polysaccharide, said method comprising the following steps:

[0047] 1) Take the Polygonatum Polygonatum raw material, grind it into coarse powder, add water and enzyme to mix evenly, keep the temperature at 40°C, and perform an enzymatic hydrolysis reaction for 2 hours, wherein the amount of water added is 8 times the mass of Polygonatum Polygonatum, and the addition of the enzyme The amount is 0.2% of Polygonatum Polygonatum mass, the enzymes are cellulase and protease, and the mass ratio of cellulase and protease is 3:1.

[0048] 2) The temperature of the enzymolysis solution was raised to 90° C., stirred and extracted for 1 hour, centrifuged, and the supernatant was taken.

[0049] 3) Continue to add process water with a material ratio of 1:8 to the filter cake, raise the temperature to 90°C, stir and extract for 1 hour. Centrifuge, take the supernatant, and repeat the above steps once more for the obtained filter cake.

[0050] 4...

technical field

[0001] The invention relates to the field of extraction of traditional Chinese medicines, in particular to a method for extracting Polygonatum polysaccharide and Polygonatum polysaccharide extract. Background technique

[0002] The traditional Chinese medicine Polygonatum Polygonatum, also known as Vitiligo, is the dry rhizome of Polygonatum modoratum (Mill.) Druce, a plant of the genus Polygonatum in the family Liliaceae. It tastes sweet and slightly bitter, and is flat and slightly warm in nature. This medicine nourishes yin and nourishes the lungs, nourishes the stomach and promotes body fluids, and has the effects of nourishing and health care, and both medicine and food. "Shen Nong's Materia Medica", "Compendium of Materia Medica" and other traditional Chinese medicine classics are listed as top grade. Modern medical research shows that Yuzhu has the functions of strengthening the heart, dilating blood vessels, lowering blood pressure, lowering blood su...

Examples

Embodiment 1

[0032] A method for extracting polygonatum polysaccharide, said method comprising the following steps:

[0033] 1) Take the Polygonatum Polygonatum raw material, grind it into coarse powder, add water and enzyme to mix evenly, keep the temperature at 40°C, and perform an enzymatic hydrolysis reaction for 2 hours, wherein the amount of water added is 8 times the mass of Polygonatum Polygonatum, and the addition of the enzyme The amount is 0.3% of Polygonatum Polygonatum mass, and the enzymes are cellulase, pectinase and hemicellulase, and the mass ratio of the cellulase, pectinase and hemicellulase is 2:1:1.

[0034] 2) The temperature of the enzymolysis solution was raised to 90° C., stirred and extracted for 1 hour, centrifuged, and the supernatant was taken.

[0035] 3) Continue to add process water with a material ratio of 1:8 to the filter cake, raise the temperature to 90°C, stir and extract for 1 hour. Centrifuge, take the supernatant, and repeat the above steps once mo...

Embodiment 2

[0039] A method for extracting polygonatum polysaccharide, said method comprising the following steps:

[0040] 1) Take the Polygonatum Polygonatum raw material, grind it into coarse powder, add water and enzyme to mix evenly, keep the temperature at 40°C, and perform an enzymatic hydrolysis reaction for 2 hours, wherein the amount of water added is 8 times the mass of Polygonatum Polygonatum, and the addition of the enzyme The amount is 0.4% of Polygonatum Polygonatum quality, and described enzyme is cellulase, pectinase, protease, and the mass ratio of described cellulase, pectinase and protease is 2:2:1.

[0041] 2) The temperature of the enzymolysis solution was raised to 90° C., stirred and extracted for 1 hour, centrifuged, and the supernatant was taken.

[0042] 3) Continue to add process water with a material ratio of 1:8 to the filter cake, raise the temperature to 90°C, stir and extract for 1 hour. Centrifuge, take the supernatant, and repeat the above steps once mo...

Embodiment 3

[0046] A method for extracting polygonatum polysaccharide, said method comprising the following steps:

[0047] 1) Take the Polygonatum Polygonatum raw material, grind it into coarse powder, add water and enzyme to mix evenly, keep the temperature at 40°C, and perform an enzymatic hydrolysis reaction for 2 hours, wherein the amount of water added is 8 times the mass of Polygonatum Polygonatum, and the addition of the enzyme The amount is 0.2% of Polygonatum Polygonatum mass, the enzymes are cellulase and protease, and the mass ratio of cellulase and protease is 3:1.

[0048] 2) The temperature of the enzymolysis solution was raised to 90° C., stirred and extracted for 1 hour, centrifuged, and the supernatant was taken.

[0049] 3) Continue to add process water with a material ratio of 1:8 to the filter cake, raise the temperature to 90°C, stir and extract for 1 hour. Centrifuge, take the supernatant, and repeat the above steps once more for the obtained filter cake.

[0050] 4...

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