Method for Determination of Gingerol Content in Ginger Medicinal Material Extract by Using One Test and Multiple Evaluation Method

Abstract

The invention discloses a method for determining the gingerol content in ginger extract by quantitative assay of multi-components by single-marker. Through the separation analysis technique of the ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometer detector and the ultraviolet detector, with the help of the quantitative assay of multi-components by single-marker, the N-vanillyl nonane amide reference substance which is stable and readily available in the sample is taken as an internal reference substance, and the relative correction factor in the compositionand the four gingerol components in the sample are established, and the content of the four gingerol components in the ginger extract are determined by calculating the relative correction factor. Themethod has the advantages of simple operation, high sensitivity, high efficiency, low cost, objective and accurate evaluation of the quality of the ginger extract, and is used for quality control, and can solve the objective and reasonable control of the quality of the ginger extract by the lack of the reference substance and is of great significance for controlling quality and ensuring efficacy.

Description

technical field

[0001] The invention specifically relates to a method for measuring the content of gingerol in ginger medicinal material extract by adopting a method of one measurement and multiple evaluations. Background technique

[0002] Ginger (Zingiber officinal Rosc), also known as ginger, dried ginger, white ginger, and ginger, belongs to the perennial herb of Zingiberaceae (Zingiberaceae), and is mainly distributed in the southwestern to southeastern regions of my country. Ginger has many and complex components, and has a unique aromatic flavor and spicy taste. This unique sensory quality mainly comes from two types of active ingredients: volatile ginger essential oil and non-volatile gingerol. Ginger essential oil is ginger Provides a unique aromatic flavor; Gingerol provides ginger with a unique spicy taste. Ginger from different origins has certain differences in medicinal properties and efficacy due to different growth environments. Studies have found that ginge...

Examples

Embodiment 1

[0054] The method for measuring the content of gingerol in the ginger medicinal material extract by the method of one test and multiple evaluations comprises the following steps:

[0055] (1) Preparation of reference substance solution

[0056] Accurately weigh five reference substances of N-vanillyl nonanamide, 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol, dissolve and dilute with methanol to prepare stock solutions of each single reference substance ; Wherein, the concentration of N-vanillyl nonanamide reference substance stock solution is 509.60 μg / mL, the concentration of 6-gingerol reference substance stock solution is 552.72 μg / mL, and the concentration of 8-gingerol reference substance stock solution is 806.68 μg / mL, the concentration of 10-gingerol reference substance stock solution was 592.31 μg / mL, and the concentration of 6-shogaol reference substance stock solution was 509.60 μg / mL.

[0057] Then measure the above-mentioned single reference substance stock so...

Embodiment 2

[0100] The method of adopting one test and multiple evaluation method to measure gingerol content in the ginger medicinal material extract is according to the steps of Example 1, the difference is only that the preparation of the test solution in step (2) is different.

[0101] The preparation of need testing solution is as follows:

[0102] Wash the fresh ginger, slice it fresh, dry it at 50°C and crush it, pass through a 50-mesh sieve to obtain dried ginger powder; weigh 175g of dried ginger powder and place it in supercritical CO 2 In the extraction device, under the pressure of 40psi, extract at 35°C for 2h, 45°C for 3h, and 55°C for 5h to obtain (three) supercritical volatile oil extracts.

[0103] Accurately weigh 0.02g (accurate to 0.00001g) of supercritical volatile oil extract in a 5.0mL volumetric flask, extract to volume with 50% methanol aqueous solution, then dilute 50 times with 50% methanol aqueous solution, take 1mL, Transfer to a 10mL measuring bottle, add 0....

Embodiment 3

[0107] The method of adopting one test and multiple evaluation method to measure gingerol content in the ginger medicinal material extract is according to the steps of Example 1, the difference is only that the preparation of the test solution in step (2) is different.

[0108] The preparation of need testing solution is as follows:

[0109] Take fresh ginger, wash it, slice it while it is fresh, dry it at 50°C and crush it, and pass through a 50-mesh sieve to obtain dried ginger powder; take 20kg of fresh ginger or dried ginger powder and place it in a subcritical extraction device, use butane as an entrainer, 40 Extract at ℃ for 4 hours to obtain the subcritical volatile oil extract.

[0110] Accurately weigh 0.02g (accurate to 0.00001g) of the subcritical method volatile oil extract in a 5.0mL volumetric flask, extract to constant volume with 50% methanol aqueous solution, then dilute 50 times with 50% methanol aqueous solution, take 1mL, Transfer to a 10mL measuring bottl...

technical field

[0001] The invention specifically relates to a method for measuring the content of gingerol in ginger medicinal material extract by adopting a method of one measurement and multiple evaluations. Background technique

[0002] Ginger (Zingiber officinal Rosc), also known as ginger, dried ginger, white ginger, and ginger, belongs to the perennial herb of Zingiberaceae (Zingiberaceae), and is mainly distributed in the southwestern to southeastern regions of my country. Ginger has many and complex components, and has a unique aromatic flavor and spicy taste. This unique sensory quality mainly comes from two types of active ingredients: volatile ginger essential oil and non-volatile gingerol. Ginger essential oil is ginger Provides a unique aromatic flavor; Gingerol provides ginger with a unique spicy taste. Ginger from different origins has certain differences in medicinal properties and efficacy due to different growth environments. Studies have found that ginge...

Examples

Embodiment 1

[0054] The method for measuring the content of gingerol in the ginger medicinal material extract by the method of one test and multiple evaluations comprises the following steps:

[0055] (1) Preparation of reference substance solution

[0056] Accurately weigh five reference substances of N-vanillyl nonanamide, 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol, dissolve and dilute with methanol to prepare stock solutions of each single reference substance ; Wherein, the concentration of N-vanillyl nonanamide reference substance stock solution is 509.60 μg / mL, the concentration of 6-gingerol reference substance stock solution is 552.72 μg / mL, and the concentration of 8-gingerol reference substance stock solution is 806.68 μg / mL, the concentration of 10-gingerol reference substance stock solution was 592.31 μg / mL, and the concentration of 6-shogaol reference substance stock solution was 509.60 μg / mL.

[0057] Then measure the above-mentioned single reference substance stock so...

Embodiment 2

[0100] The method of adopting one test and multiple evaluation method to measure gingerol content in the ginger medicinal material extract is according to the steps of Example 1, the difference is only that the preparation of the test solution in step (2) is different.

[0101] The preparation of need testing solution is as follows:

[0102] Wash the fresh ginger, slice it fresh, dry it at 50°C and crush it, pass through a 50-mesh sieve to obtain dried ginger powder; weigh 175g of dried ginger powder and place it in supercritical CO 2 In the extraction device, under the pressure of 40psi, extract at 35°C for 2h, 45°C for 3h, and 55°C for 5h to obtain (three) supercritical volatile oil extracts.

[0103] Accurately weigh 0.02g (accurate to 0.00001g) of supercritical volatile oil extract in a 5.0mL volumetric flask, extract to volume with 50% methanol aqueous solution, then dilute 50 times with 50% methanol aqueous solution, take 1mL, Transfer to a 10mL measuring bottle, add 0....

Embodiment 3

[0107] The method of adopting one test and multiple evaluation method to measure gingerol content in the ginger medicinal material extract is according to the steps of Example 1, the difference is only that the preparation of the test solution in step (2) is different.

[0108] The preparation of need testing solution is as follows:

[0109] Take fresh ginger, wash it, slice it while it is fresh, dry it at 50°C and crush it, and pass through a 50-mesh sieve to obtain dried ginger powder; take 20kg of fresh ginger or dried ginger powder and place it in a subcritical extraction device, use butane as an entrainer, 40 Extract at ℃ for 4 hours to obtain the subcritical volatile oil extract.

[0110] Accurately weigh 0.02g (accurate to 0.00001g) of the subcritical method volatile oil extract in a 5.0mL volumetric flask, extract to constant volume with 50% methanol aqueous solution, then dilute 50 times with 50% methanol aqueous solution, take 1mL, Transfer to a 10mL measuring bottl...

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