Microalgae purification medium and method for isolating and purifying microalgae

Abstract

The invention relates to a microalgae purification culture medium and a method for sterilizing, separating and purifying microalgae, belonging to the field of microalgae biotechnology. The preparationmethod of the microalgae purification culture medium comprises the following steps: preparing a microalgae liquid inorganic culture medium, adding 1 to 1.5 wt% of high-purity agarose into the liquidinorganic culture medium to obtain a mixed solution, sterilizing the mixed solution, and preparing a microalgae solid culture medium. The microalgae purification culture medium used for purifying themicroalgae does not have adverse effects on the microalgae itself, and the microalgae purification culture medium has the advantages of simple operation, no need to culture the microalgae for many times, high work efficiency and low cost.

Description

technical field

[0001] The invention relates to a medium for purifying microalgae and a method for removing bacteria, separating and purifying microalgae, and belongs to the technical field of microalgae biology. Background technique

[0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. The polysaccharides, proteins, pigments, etc. produced by cell metabolism make them useful in the fields of food, medicine, genetic engineering, and liquid fuels. Very good development prospects. The pure culture of microalgae is essential to the study of nutrition, physiology and biochemistry of marine microalgae, but during the culture process, microalgae are usually polluted by other microorganisms, which makes its growth rate and culture Density is affected. Sterile pure algae is the basis for in-depth research on algae physiology and genetics, so the cultivation and preservation of pure speci...

Examples

preparation example Construction

[0025] In order to solve the first technical problem of the present invention, the preparation method of microalgae purification culture medium of the present invention comprises the steps:

[0026] Weigh a considerable amount of agarose, put it into a glass chromatographic column, and continuously drop in pure water to wash it overnight for later use.

[0027] The microalgae liquid inorganic culture medium is prepared, and then 1-1.5 wt% of high-purity agarose is added to the liquid inorganic culture medium to obtain a mixed liquid, and the microalgae solid culture medium is obtained after the mixed liquid is sterilized.

[0028] Preferably, the high-purity agarose is agarose that has been cleaned and purified by a chromatographic column. The preferred method of cleaning and purifying the chromatographic column is: put the agarose into the chromatographic column, and use 0.6-1.0 ml of water per minute The flow rate per gram of agarose is washed for more than 10 hours, and the...

Embodiment 1

[0039] The method of purifying Haematococcus pluvialis with BBM basic solid medium:

[0040] Step 1: Weigh 3.0g of agarose, put it into an empty glass chromatographic column with a size of φ2cmX20cm, pour pure water into the chromatographic column continuously at a flow rate of 2.5ml / min, and wash the agarose overnight for later use.

[0041] Step 2: Prepare 200ml of BBM liquid medium according to the conventional method;

[0042] Step 3: Add 1.5w%, that is, about 3.0 g of the agarose cleaned in step 1 to the 200ml BBM liquid medium prepared in step 2, and perform a sterilized inverted plate operation to obtain a BBM solid medium;

[0043] Step 4: Carry out the inoculation operation on the aseptic operating table, adopt the method of spreading and inoculating, and the algal seed liquid used in the inoculation is Haematococcus pluvialis FACHB-712 purchased from the algae stock of the Institute of Hydrobiology, Chinese Academy of Sciences, The inoculum volume is 100 μl;

[004...

technical field

[0001] The invention relates to a medium for purifying microalgae and a method for removing bacteria, separating and purifying microalgae, and belongs to the technical field of microalgae biology. Background technique

[0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. The polysaccharides, proteins, pigments, etc. produced by cell metabolism make them useful in the fields of food, medicine, genetic engineering, and liquid fuels. Very good development prospects. The pure culture of microalgae is essential to the study of nutrition, physiology and biochemistry of marine microalgae, but during the culture process, microalgae are usually polluted by other microorganisms, which makes its growth rate and culture Density is affected. Sterile pure algae is the basis for in-depth research on algae physiology and genetics, so the cultivation and preservation of pure speci...

Examples

preparation example Construction

[0025] In order to solve the first technical problem of the present invention, the preparation method of microalgae purification culture medium of the present invention comprises the steps:

[0026] Weigh a considerable amount of agarose, put it into a glass chromatographic column, and continuously drop in pure water to wash it overnight for later use.

[0027] The microalgae liquid inorganic culture medium is prepared, and then 1-1.5 wt% of high-purity agarose is added to the liquid inorganic culture medium to obtain a mixed liquid, and the microalgae solid culture medium is obtained after the mixed liquid is sterilized.

[0028] Preferably, the high-purity agarose is agarose that has been cleaned and purified by a chromatographic column. The preferred method of cleaning and purifying the chromatographic column is: put the agarose into the chromatographic column, and use 0.6-1.0 ml of water per minute The flow rate per gram of agarose is washed for more than 10 hours, and the...

Embodiment 1

[0039] The method of purifying Haematococcus pluvialis with BBM basic solid medium:

[0040] Step 1: Weigh 3.0g of agarose, put it into an empty glass chromatographic column with a size of φ2cmX20cm, pour pure water into the chromatographic column continuously at a flow rate of 2.5ml / min, and wash the agarose overnight for later use.

[0041] Step 2: Prepare 200ml of BBM liquid medium according to the conventional method;

[0042] Step 3: Add 1.5w%, that is, about 3.0 g of the agarose cleaned in step 1 to the 200ml BBM liquid medium prepared in step 2, and perform a sterilized inverted plate operation to obtain a BBM solid medium;

[0043] Step 4: Carry out the inoculation operation on the aseptic operating table, adopt the method of spreading and inoculating, and the algal seed liquid used in the inoculation is Haematococcus pluvialis FACHB-712 purchased from the algae stock of the Institute of Hydrobiology, Chinese Academy of Sciences, The inoculum volume is 100 μl;

[004...

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