A rapid detection method for free radical markers collected by breathing

Abstract

The present invention discloses a rapid detection method for collecting free radical markers by breathing, which specifically includes the following steps: preparing items required for detection; reacting a reagent tube; using an OXI‑CHECKER breath-type rapid free radical detector to analyze the color change of a reagent in the reagent tube and obtain a score. The present invention can quickly collect condensate and free radical markers (aldehydes) in human exhaled gas, accurately analyze the color and RGB values ​​of the reagent, and use a calculation formula to convert the final free radical evaluation score. The test result can be accurately obtained within 3 minutes, which is faster and more convenient than traditional blood and urine tests, and does not require professional operation. The state of free radicals can be accurately self-detected anytime and anywhere.

Description

Technical Field

[0001] The invention relates to the technical field of biology, and in particular to a rapid detection method for collecting free radical markers by breathing. Background Art

[0002] When a covalent bond between chemical entities is broken, one of the electrons is left behind, and a new atom is formed. This is when free radicals are easily formed, and due to the presence of the lone electron, free radicals are highly reactive. Polyunsaturated fatty acids are abundant in cell membranes and low-density lipoproteins. Polyunsaturated fatty acids make cell membranes mobile, and free radicals are particularly fond of attacking cells and taking electrons from their plasma membranes. This is called lipid peroxidation. Reactive oxygen species target the carbon-carbon double bonds of polyunsaturated fatty acids because the carbon-carbon double bond weakens the carbon-hydrogen single bond, which allows free radicals to easily dissociate the carbon-hydrogen bond. The free radical will take a single electron from the hydrogen atom associated with the carbon atom at the double bond position, and then a lone electron is left on the carbon atom, and a free radical is formed. In an effort to stabilize the carbon-centered free radical, the molecules rearrange themselves into a new molecule called a conjugated diene, which reacts easily with oxygen to form a peroxyl radical. The peroxyl radical then grabs an electron from another lipid molecule during amplification. This process continues in the next chain reaction. When a fatty acid is peroxidized, it breaks down into aldehydes, which can then be excreted from the body in the exhaled breath.

[0003] Free radicals play a very important role in both health and disease processes. They are involved in human disease processes and are important for metabolic processes. External factors such as diet, activity, stress, chemicals and pollutants can cause our bodies to produce too many free radicals, which can damage healthy cells, which can lead to health problems such as cardiovascular disease, cancer and rapid aging.

[0004] Traditional free radical detection requires professional medical institutions and professionals to obtain results, which takes a long time to detect the results. It is not fast and convenient enough, and it is not possible to self-detect the free radical status at any time, which is very inconvenient. Therefore, the present invention provides a rapid detection method for collecting free radical markers by breathing to solve the problems raised in the above background technology. Summary of the invention

[0005] The purpose of the present invention is to provide a rapid detection method for free radical markers collected by breathing to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A rapid detection method for collecting free radical markers by breathing method, specifically comprising the following steps:

[0008] 1. Prepare items needed for testing

[0009] Prepare an OXI-CHECKER breath-type rapid free radical detector reagent tube containing a micro-particle catalytic biosensor free radical detection photosensitive powder reagent and an OXI-CHECKER breath-type rapid free radical detector;

[0010] 2. Reagent tube reaction

[0011] a. The user holds the OXI-CHECKER breath-type rapid free radical detector reagent tube and blows air into the OXI-CHECKER breath-type rapid free radical detector reagent tube;

[0012] b. The free radical markers in the exhaled gas of the human body react with the free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector;

[0013] c. The free radical detection photosensitive powder reagent gradually changes color after reacting with the free radical marker, and the color gradually changes from yellow to pink, pink purple and finally to purple;

[0014] 3. OXI-CHECKER breath-type rapid free radical detector analyzes the color change of the reagent in the reagent tube and gives a score

[0015] a. The reagents in the reagent tube after the reaction have good correlation in the spectral regions of 440nm, 530nm, and 570nm, and the concentration and absorbance (transmittance) reach the highest correlation at the wavelength of 440nm;

[0016] b. Insert the reagent tube after the reaction in step 2 into the OXI-CHECKER breath-type rapid free radical detector, and the OXI-CHECKER breath-type rapid free radical detector analyzes the RGB score after the reaction in the reagent tube;

[0017] c. According to step a, the most photosensitivity colors in RGB at wavelengths of 440nm, 530nm, and 570nm are B (blue) and G (green). The OXI-CHECKER breath-type rapid free radical detector automatically sets the ratio of B and G in the reagent color to read. The ratio of B and G is used to calculate a score of 0-1000. This score is related to the content of free radical markers in the exhaled gas. The higher the score, the higher the free radical level.

[0018] As a further solution of the present invention, the specific method for calculating the score in step 3 is as follows:

[0019] a. First calculate the RGB score of the reagent: the instrument reads R=X, G=Y, B=Z score;

[0020] b. Calculate the weights of the RGB values: GP = G / (X+Y+Z), BP = B / (X+Y+Z);

[0021] cG score: Gscore = GP*K+M;

[0022] dB score: Bscore = BP*K+M;

[0023] e. Free radical score: Score = (Gscore + Bscore) / 2;

[0024] Among them, K is the slope and M is the intercept.

[0025] As a further solution of the present invention, the formula of the micro-particle catalytic biosensor free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector in step one is as follows: silicon dioxide, basic fuchsin, sodium metabisulfite, 85% phosphoric acid, deionized water, activated carbon or bone charcoal.

[0026] As a further embodiment of the present invention, the particle size of silicon dioxide in the photosensitive powder reagent is between 20-50 meshes.

[0027] As a further solution of the present invention, the amounts of the raw materials in the photosensitive powder reagent are as follows: 2.5-5 g of basic fuchsin, 10 g of sodium pyrosulfite, 20 ml of 85% phosphoric acid, 1000 ml of deionized water, and 30 g of activated carbon or bone charcoal.

[0028] As a further solution of the present invention, the preparation process of the photosensitive powder reagent is as follows:

[0029] (1) Silica pretreatment

[0030] 1) Dilute 85% phosphoric acid into a 2%-5% phosphoric acid solution;

[0031] 2) 2%-5% phosphoric acid and silicon dioxide are mixed in a ratio of 2:1;

[0032] 3) Mix the two, stir, and place in a constant temperature drying oven to dry until the target weight is reached;

[0033] (2) Preparation of fuchsin liquid reagent

[0034] 1) First extract the required amount of deionized water, and then add sodium metabisulfite;

[0035] 2) After the sodium metabisulfite is completely dissolved, add basic fuchsin while stirring;

[0036] 3) After the basic fuchsin is completely dissolved, add activated carbon or bone charcoal, stir, let stand, and filter;

[0037] 4) then adjusting the pH value of the solution after filtration in step 3) with 85% phosphoric acid to ensure that the pH value of the solution is between 1.75 and 1.85;

[0038] (3) Reagent mixing

[0039] 1) mixing the pretreated silicon dioxide with the liquid reagent prepared in step (2) in a ratio of 1:2, and diluting the prepared liquid reagent with a phosphoric acid solution before mixing to ensure that the pH value of the final liquid is between 1.75 and 1.85;

[0040] 2) After mixing evenly, place in a constant temperature drying oven and dry to the target weight;

[0041] 3) After drying and cooling, pour into a brown glass bottle, fill with argon gas to expel the air, cover with a lid and seal for storage.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] The present invention can quickly collect condensate and free radical markers (aldehydes) in human exhaled gas, accurately analyze the color and RGB value of the reagent, and use a calculation formula to convert the final free radical assessment score. The test result can be accurately obtained within 3 minutes. It is faster and more convenient than traditional blood and urine tests, does not require professional operation, and can accurately self-detect the status of free radicals anytime and anywhere. DETAILED DESCRIPTION

[0044] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0045] Example 1

[0046] A rapid detection method for collecting free radical markers by breathing method, specifically comprising the following steps:

[0047] 1. Prepare items needed for testing

[0048] Prepare an OXI-CHECKER breath-type rapid free radical detector reagent tube containing a micro-particle catalytic biosensor free radical detection photosensitive powder reagent and an OXI-CHECKER breath-type rapid free radical detector;

[0049] 2. Reagent tube reaction

[0050] a. The user holds the OXI-CHECKER breath-type rapid free radical detector reagent tube and blows air into the OXI-CHECKER breath-type rapid free radical detector reagent tube;

[0051] b. The free radical markers in the exhaled gas of the human body react with the free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector;

[0052] c. The free radical detection photosensitive powder reagent gradually changes color after reacting with the free radical marker, and the color gradually changes from yellow to pink, pink purple and finally to purple;

[0053] 3. Use OXI-CHECKER breath-type rapid free radical detector to analyze the color change of the reagent in the reagent tube and get a score

[0054] a. The reagents in the reagent tube after the reaction have good correlation in the spectral regions of 440nm, 530nm, and 570nm, and the concentration and absorbance (transmittance) reach the highest correlation at the wavelength of 440nm;

[0055] b. Insert the reagent tube after the reaction in step 2 into the OXI-CHECKER breath-type rapid free radical detector, and the OXI-CHECKER breath-type rapid free radical detector analyzes the RGB score after the reaction in the reagent tube;

[0056] c. According to step a, the most photosensitivity colors in RGB at wavelengths of 440nm, 530nm, and 570nm are B (blue) and G (green). The OXI-CHECKER breath-type rapid free radical detector automatically sets the ratio of B and G in the reagent color to read. The ratio of B and G is used to calculate a score of 0-1000. This score is related to the content of free radical markers in the exhaled gas. The higher the score, the higher the free radical level.

[0057] The specific method for calculating the score in step 3 is as follows:

[0058] a. First calculate the RGB score of the reagent: the instrument reads R=X, G=Y, B=Z score;

[0059] b. Calculate the weights of the RGB values: GP = G / (X+Y+Z), BP = B / (X+Y+Z);

[0060] cG score: Gscore = GP*K+M;

[0061] dB score: Bscore = BP*K+M;

[0062] e. Free radical score: Score = (Gscore + Bscore) / 2;

[0063] Among them, K is the slope and M is the intercept.

[0064] The formula of the micro-particle catalytic biosensor free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector in step 1 is as follows: silicon dioxide, basic fuchsin, sodium metabisulfite, 85% phosphoric acid, deionized water, and activated carbon.

[0065] The particle size of silicon dioxide in the photosensitive powder reagent is 20 meshes.

[0066] The amounts of the raw materials in the photosensitive powder reagent are as follows: 2.5 g of basic fuchsin, 10 g of sodium pyrosulfite, 20 ml of 85% phosphoric acid, 1000 ml of deionized water, and 30 g of activated carbon.

[0067] The preparation process of the photosensitive powder reagent is as follows:

[0068] (1) Silica pretreatment

[0069] 1) Dilute 85% phosphoric acid into a 2%-5% phosphoric acid solution;

[0070] 2) 2%-5% phosphoric acid and silicon dioxide are mixed in a ratio of 2:1;

[0071] 3) Mix the two, stir, and place in a constant temperature drying oven to dry until the target weight is reached.

[0072] (2) Preparation of fuchsin liquid reagent

[0073] 1) First extract the required amount of deionized water, and then add sodium metabisulfite;

[0074] 2) After the sodium metabisulfite is completely dissolved, add basic fuchsin while stirring;

[0075] 3) After the basic fuchsin is completely dissolved, add activated carbon, stir, let stand, and filter;

[0076] 4) Then, 85% phosphoric acid is used to adjust the pH value of the solution after filtration in step 3) to ensure that the pH value of the solution is between 1.75 and 1.85.

[0077] (3) Reagent mixing

[0078] 1) mixing the pretreated silicon dioxide with the liquid reagent prepared in step (2) in a ratio of 1:2, and diluting the prepared liquid reagent with a phosphoric acid solution before mixing to ensure that the pH value of the final liquid is between 1.75 and 1.85;

[0079] 2) After mixing evenly, place in a constant temperature drying oven and dry to the target weight;

[0080] 3) After drying and cooling, pour into a brown glass bottle, fill with argon gas to expel the air, cover with a lid and seal for storage.

[0081] Example 2

[0082] A rapid detection method for collecting free radical markers by breathing method, specifically comprising the following steps:

[0083] 1. Prepare items needed for testing

[0084] Prepare an OXI-CHECKER breath-type rapid free radical detector reagent tube containing a micro-particle catalytic biosensor free radical detection photosensitive powder reagent and an OXI-CHECKER breath-type rapid free radical detector;

[0085] 2. Reagent tube reaction

[0086] a. The user holds the OXI-CHECKER breath-type rapid free radical detector reagent tube and blows air into the OXI-CHECKER breath-type rapid free radical detector reagent tube;

[0087] b. The free radical markers in the exhaled gas of the human body react with the free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector;

[0088] c. The free radical detection photosensitive powder reagent gradually changes color after reacting with the free radical marker, and the color gradually changes from yellow to pink, pink purple and finally to purple;

[0089] 3. Use OXI-CHECKER breath-type rapid free radical detector to analyze the color change of the reagent in the reagent tube and get a score

[0090] a. The reagents in the reagent tube after the reaction have good correlation in the spectral regions of 440nm, 530nm, and 570nm, and the concentration and absorbance (transmittance) reach the highest correlation at the wavelength of 440nm;

[0091] b. Insert the reagent tube after the reaction in step 2 into the OXI-CHECKER breath-type rapid free radical detector, and the OXI-CHECKER breath-type rapid free radical detector analyzes the RGB score after the reaction in the reagent tube;

[0092] c. According to step a, the most photosensitivity colors in RGB at wavelengths of 440nm, 530nm, and 570nm are B (blue) and G (green). The OXI-CHECKER breath-type rapid free radical detector automatically sets the ratio of B and G in the reagent color to read. The ratio of B and G is used to calculate a score of 0-1000. This score is related to the content of free radical markers in the exhaled gas. The higher the score, the higher the free radical level.

[0093] The specific method for calculating the score in step 3 is as follows:

[0094] a. First calculate the RGB score of the reagent: the instrument reads R=X, G=Y, B=Z score;

[0095] b. Calculate the weights of the RGB values: GP = G / (X+Y+Z), BP = B / (X+Y+Z);

[0096] cG score: Gscore = GP*K+M;

[0097] dB score: Bscore = BP*K+M;

[0098] e. Free radical score: Score = (Gscore + Bscore) / 2;

[0099] Among them, K is the slope and M is the intercept.

[0100] The formula of the micro-particle catalytic biosensor free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector in step 1 is as follows: silicon dioxide, basic fuchsin, sodium metabisulfite, 85% phosphoric acid, deionized water, and bone charcoal.

[0101] The particle size of silicon dioxide in the photosensitive powder reagent is 50 meshes.

[0102] The amounts of the raw materials in the photosensitive powder reagent are as follows: 5 g of basic fuchsin, 10 g of sodium pyrosulfite, 20 ml of 85% phosphoric acid, 1000 ml of deionized water, and 30 g of bone charcoal.

[0103] The preparation process of the photosensitive powder reagent is as follows:

[0104] (1) Silica pretreatment

[0105] 1) Dilute 85% phosphoric acid into a 2%-5% phosphoric acid solution;

[0106] 2) 2%-5% phosphoric acid and silicon dioxide are mixed in a ratio of 2:1;

[0107] 3) Mix the two, stir, and place in a constant temperature drying oven to dry until the target weight is reached.

[0108] (2) Preparation of fuchsin liquid reagent

[0109] 1) First extract the required amount of deionized water, and then add sodium metabisulfite;

[0110] 2) After the sodium metabisulfite is completely dissolved, add basic fuchsin while stirring;

[0111] 3) After the basic fuchsin is completely dissolved, add bone char, stir, let stand, and filter;

[0112] 4) then adjusting the pH value of the solution after filtration in step 3) with 85% phosphoric acid to ensure that the pH value of the solution is between 1.75 and 1.85;

[0113] (3) Reagent mixing

[0114] 1) mixing the pretreated silicon dioxide with the liquid reagent prepared in step (2) in a ratio of 1:2, and diluting the prepared liquid reagent with a phosphoric acid solution before mixing to ensure that the pH value of the final liquid is between 1.75 and 1.85;

[0115] 2) After mixing evenly, place in a constant temperature drying oven and dry to the target weight;

[0116] 3) After drying and cooling, pour into a brown glass bottle, fill with argon gas to expel the air, cover with a lid and seal for storage.

[0117] The present invention can quickly collect condensate and free radical markers (aldehydes) in human exhaled gas, accurately analyze the color and RGB value of the reagent, and use a calculation formula to convert the final free radical assessment score. The test result can be accurately obtained within 3 minutes. It is faster and more convenient than traditional blood and urine tests, does not require professional operation, and the status of free radicals can be self-tested anytime and anywhere.

[0118] The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the above embodiments.

Claims

1. A rapid detection method for collecting free radical markers by breathing, characterized in that: The specific steps include:

1. Prepare items needed for testing Prepare an OXI-CHECKER breath-type rapid free radical detector reagent tube containing a micro-particle catalytic biosensor free radical detection photosensitive powder reagent and an OXI-CHECKER breath-type rapid free radical detector; 2. Reagent tube reaction a. The user holds the OXI-CHECKER breath-type rapid free radical detector reagent tube and blows air into the OXI-CHECKER breath-type rapid free radical detector reagent tube; b. The free radical markers in the exhaled gas of the human body react with the free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector; c. The free radical detection photosensitive powder reagent gradually changes color after reacting with the free radical marker, and the color gradually changes from yellow to pink, pink purple and finally to purple; 3. Use OXI-CHECKER breath-type rapid free radical detector to analyze the color change of the reagent in the reagent tube and get a score a. The reagents in the reagent tube after the reaction have good correlation in the spectral regions of 440nm, 530nm, and 570nm, and the concentration and absorbance reach the highest correlation at the wavelength of 440nm; b. Insert the reagent tube after the reaction in step 2 into the OXI-CHECKER breath-type rapid free radical detector, and the OXI-CHECKER breath-type rapid free radical detector analyzes the RGB score after the reaction in the reagent tube; c. According to step a, the colors with the strongest photosensitivity in RGB at wavelengths of 440nm, 530nm, and 570nm are B and G. The OXI-CHECKER breath-type rapid free radical detector automatically sets and reads the proportion of B and G in the reagent color, and uses the proportion of B and G to calculate a score of 0-1000; The formula of the micro-particle catalytic biosensor free radical detection photosensitive powder reagent in the reagent tube of the OXI-CHECKER breath-type rapid free radical detector in step 1 is as follows: silicon dioxide, basic fuchsin, sodium metabisulfite, 85% phosphoric acid, deionized water, and activated carbon.

2. A rapid detection method for free radical markers collected by breathing method according to claim 1, characterized in that: The specific method for calculating the score in step 3 is as follows: a. First calculate the RGB score of the reagent: the instrument reads R=X, G=Y, B=Z score; b. Calculate the weights of the RGB values: GP = G / (X+Y+Z), BP = B / (X+Y+Z); cG score: Gscore = GP*K+M; dB score: Bscore = BP*K+M; e. Free radical score: Score = (Gscore + Bscore) / 2; Among them, K is the slope and M is the intercept.

3. A rapid detection method for free radical markers collected by breathing method according to claim 1, characterized in that: The particle size of silicon dioxide in the photosensitive powder reagent is between 20-50 meshes.

4. A rapid detection method for collecting free radical markers by breathing method according to any one of claims 1 to 3, characterized in that: The preparation process of the photosensitive powder reagent is as follows: (1) Silica pretreatment 1) Dilute 85% phosphoric acid into a 2%-5% phosphoric acid solution; 2) 2%-5% phosphoric acid and silicon dioxide are mixed in a ratio of 2:1; 3) Mix the two, stir, and place in a constant temperature drying oven to dry until the target weight is reached; (2) Preparation of fuchsin liquid reagent 1) First extract the required amount of deionized water, and then add sodium metabisulfite; 2) After the sodium metabisulfite is completely dissolved, add basic fuchsin while stirring; 3) After the basic fuchsin is completely dissolved, add activated carbon, stir, let stand, and filter; 4) then adjusting the pH value of the solution after filtration in step 3) with 85% phosphoric acid to ensure that the pH value of the solution is between 1.75 and 1.85; (3) Reagent mixing 1) mixing the pretreated silicon dioxide with the liquid reagent prepared in step (2) in a ratio of 1:2, and diluting the prepared liquid reagent with a phosphoric acid solution before mixing to ensure that the pH value of the final liquid is between 1.75 and 1.85; 2) After mixing evenly, place in a constant temperature drying oven and dry to the target weight; 3) After drying and cooling, pour into a brown glass bottle, fill with argon gas to expel the air, cover with a lid and seal for storage.