Multifunctional brain stereotaxic device

Abstract

The present invention relates to a multifunctional brain stereo positioning device, including a fixing mechanism, a three-dimensional positioning mechanism and an auxiliary mechanism, wherein the fixing mechanism, the three-dimensional positioning mechanism and the auxiliary mechanism are all arranged on a bottom plate, the three-dimensional positioning mechanism is arranged above the fixing mechanism, a transverse slide groove is provided on the bottom plate, and the auxiliary mechanism is slidably arranged in the transverse slide groove, and can slide to the top of the fixing mechanism and the bottom of the three-dimensional positioning mechanism. The present invention is scientifically and reasonably designed and convenient to use. The fixing mechanism and the three-dimensional coordinate positioning mechanism are used to fix the experimental mouse, and the three-dimensional coordinates can be accurately and effectively adjusted and fixed. At the same time, a rotating limit plate is provided, which is suitable for drill bits and micro-injections of various specifications, and effectively improves the accuracy and efficiency of the experiment. At the same time, the experimental support unit and the auxiliary unit of the auxiliary mechanism realize the head skin support function of the experimental mouse, and the auxiliary unit is used to realize residual blood collection and experimental mouse heartbeat monitoring. It is an integrated multifunctional brain stereo positioning device with high value and good practical use effect.

Description

Technical Field

[0001] The invention belongs to the field of animal experiments and relates to a brain stereotaxic device, in particular to a multifunctional brain stereotaxic device. Background Art

[0002] Stereotaxic apparatus, also known as brain fixation device, uses a three-dimensional coordinate system defined by the marks on the outside of the skull or other reference points to determine the position of certain subcortical neural structures, so as to conduct targeted stimulation, destruction, drug injection, potential guidance and other studies under non-direct exposure. Animal brain stereotaxic apparatus is an important research device in the fields of neuroanatomy, neurophysiology, neuropharmacology and neurosurgery. It is used for targeted injection, stimulation, destruction, potential guidance and other operations on neural structures. It can be used to establish animal models of Parkinson's disease, epilepsy, brain tumor models, learning and memory, brain neural stem cell transplantation, cerebral ischemia and other studies.

[0003] There are some technical problems in the application of the brain stereotaxic instrument in the prior art:

[0004] 1. In traditional stereotaxic brain positioners, there is no structure on the three-dimensional positioning rod that can lock the scale position. After the coordinate adjustment rod is adjusted, if the adjustment rod is accidentally touched, the coordinate of the adjustment rod will deviate, resulting in inaccurate positioning, which requires repositioning.

[0005] 2. In the process of constructing an animal model, a traditional stereotaxic apparatus needs to select different drill bits for positioning and drilling according to the size of the selected animal's head and the hardness of the animal's skull, and select microsyringes of different specifications to inject the solution required for model construction into the positioning hole. This requires the replacement of drill bits and microsyringes of different models or specifications during actual operation. When replacing drill bits and syringe needles, traditional stereotaxic apparatuses need to dismantle and replace the original drill bits or syringes, which increases labor costs and operation time. In addition, the drill bit used for drilling positioning holes in the prior art is separated from the stereotaxic apparatus itself, and the drill bit needs to be manually held to drill holes. When drilling holes, the drill bit cannot be manually held to drill holes directly above the positioning holes, which can easily cause the holes drilled by the drill bit to deviate from the positioning holes by a certain angle, making the drilling inaccurate. In addition, the stability of the hand-held drill bit during drilling can also cause the hole position to deviate, affecting the drilling accuracy.

[0006] 3. The animal brain stereotaxic operation first requires opening an opening on the top of the animal's head, peeling off the tissue between the skull and the skin to expose the skull, locating the coordinate points on the skull according to the reference coordinates, drilling holes at the positioning points with a drill, and finally injecting the reagents needed to build the model into the holes with a microsyringe. In the brain stereotaxic operation, when the skin on the top of the animal's head is opened and subsequent operations are performed, the skin on both sides of the opening needs to be stretched to the sides to avoid covering the skull and affecting the positioning, drilling and injection. The traditional brain stereotaxic device is not designed to stretch the skin at the opening. It is necessary to manually use tweezers to stretch the skin at the opening to both sides to expose the skull. The tweezers are not firm enough to fix the skin, and the skin on both sides of the opening is easy to loosen, affecting the subsequent positioning and drilling operations;

[0007] 4. Since the head of an animal is densely covered with capillaries, when the skin on the top of the animal's head is cut and the tissue is peeled off, the capillaries are often cut, causing the capillaries to rupture and bleed. In addition, various blood vessels are distributed in the brain area below the skull of the animal. When the drill bit drills holes at the coordinate points, the drill bit is also easy to touch the capillaries below the skull, causing the blood vessels in the positioning hole to rupture and bleed. After bleeding, the blood will cover the positioning hole, making the positioning hole unclear, affecting positioning and drilling;

[0008] 5. The traditional stereotaxic device does not have a device for collecting and processing residual blood. When the capillaries in the animal's head bleed, it is necessary to manually clamp hemostatic sponges or cotton balls to stop bleeding. The hemostatic sponges or cotton balls are not thorough enough to stop bleeding, and it is necessary to stop bleeding repeatedly, which consumes manpower costs and operation time, affecting the smooth progress of the stereotaxic operation.

[0009] 6. During the animal brain stereotaxic process, due to individual differences among animals, various objective reasons such as the dosage of anesthetics and the operator's technique may lead to the death of the animal. The traditional brain stereotaxic device is not designed with a real-time monitoring device for the animal's heartbeat status. It cannot monitor the animal's heartbeat status at any time, and cannot promptly detect signs of physical weakness in the animal and provide timely rescue, which may lead to the animal's death and affect the experimental operation. Summary of the invention

[0010] The purpose of the present invention is to overcome the shortcomings of the prior art and to provide a multifunctional brain stereotaxic positioning device that can accurately locate three-dimensional scales and effectively fix three-dimensional coordinate axes, and can realize integrated precise positioning for the use of drill bits and syringes of multiple specifications, and can effectively fix experimental animals during the experiment, can fix the animal's head skin after opening it, can collect residual blood during the experimental operation, and monitor the animal's heartbeat status in real time.

[0011] The present invention solves the technical problem by adopting the following technical solutions:

[0012] A multifunctional brain stereo positioning device comprises a fixing mechanism, a three-dimensional positioning mechanism and an auxiliary mechanism, wherein the fixing mechanism, the three-dimensional positioning mechanism and the auxiliary mechanism are all arranged on a base plate, the three-dimensional positioning mechanism is arranged above the fixing mechanism, a transverse slide groove is provided on the base plate, and the auxiliary mechanism is slidably arranged in the transverse slide groove and can slide to the top of the fixing mechanism and the bottom of the three-dimensional positioning mechanism.

[0013] Moreover, the three-dimensional positioning mechanism includes an adjustable front and rear coordinate rod, an adjustable left and right coordinate rod, and an adjustable upper and lower coordinate rod. The adjustable front and rear coordinate rod is arranged on a base, the adjustable front and rear coordinate rod is connected to the adjustable upper and lower coordinate rod, the adjustable upper and lower coordinate rod is connected to the adjustable left and right coordinate rod, the adjustable left and right coordinate rod is connected to a positioning rod linkage control shaft, a rotating shaft is installed at the bottom end of the positioning rod linkage control shaft, a rotating limit plate is coaxially and detachably installed on the rotating shaft, and a plurality of unit slots for placing drill bits and syringes are opened on the rotating limit plate.

[0014] Moreover, the front and rear adjustment coordinate rods are arranged in the longitudinal sliding groove of the base plate, and scale lines are arranged on the rod body of the front and rear adjustment coordinate rods. The left and right adjustment coordinate rods and the upper and lower adjustment coordinate rods are both provided with rotating shaft rods, and the rotating shaft rods are coaxially threaded in a sleeve with scales. A caliper is slidably sleeved on the rotating shaft rod, and a fixed shaft rod with a spring is arranged on one side of the caliper. On the rotating shaft rod, a clamp for locking the rotating shaft rod is arranged on the other side of the caliper.

[0015] Moreover, the auxiliary mechanism includes an experimental support unit and an auxiliary unit. The experimental support mechanism is arranged on the fixing mechanism, and the auxiliary mechanism is arranged on the experimental support mechanism. The experimental support mechanism includes a support frame, a spring chain and a herringbone skin support frame. The support frame is arranged on the base plate. One end of the spring chain is movably fixed to the upper part of the support plate by a screw, and the lower part is hinged with the herringbone skin support frame. The herringbone skin support frame includes two symmetrical herringbone support plates, and screws are correspondingly installed on the two herringbone support plates. The ends of the two screws are connected to the two ends of the spring.

[0016] Moreover, the auxiliary unit includes a support plate, a negative pressure aspirator and an animal heart rate collector. The support plate is arranged on a support frame, and the negative pressure aspirator and the animal heart rate collector are placed on the support frame. One end of the negative pressure aspirator is provided with an interface for connecting a backflow hose, and the other end is connected to a residual blood collection bottle.

[0017] Moreover, the fixing mechanism includes a base plate, a limit block and a limit rod. The two limit blocks are symmetrically arranged on the base plate. Limit slots are opened on the limit blocks. The two limit rods are detachably and symmetrically arranged in the corresponding limit slots. The limit rods are fixed by screws arranged on the limit blocks.

[0018] The advantages and positive effects of the present invention are:

[0019] 1. This device uses a three-dimensional coordinate positioning mechanism to achieve accurate fixation of the three-dimensional coordinates, and realizes the fixing function through a clamp set on the rotating shaft rod, which solves the problem that the scale of the coordinate rod is easily deviated due to the shaking of the work table.

[0020] 2. The device is provided with a rotating shaft at the bottom end of the positioning rod linkage control shaft, on which a rotating limit plate is coaxially and detachably installed, and on which a number of unit slots for placing drill bits and syringes are provided, and the drilling drill bits and micro-injections can be placed according to actual experimental needs, thereby solving the problem of deviation in drilling and injection, making drilling and micro-injection more precise, and eliminating the need for manual position adjustment, thus saving manpower and time costs, and effectively improving the effectiveness of the experiment.

[0021] 3. The fixing device of the device is provided with a bottom plate, a limit block and a limit rod. The head of the experimental mouse is fixed by adjusting the limit rod and fixing it with screws.

[0022] 4. This device is provided with an auxiliary device on the traditional positioning device. The auxiliary mechanism includes a negative pressure aspirator and an animal heart rate collector. One end of the negative pressure aspirator is provided with an interface for connecting a backflow hose, and the other end is connected to a blood collection bottle. When residual blood appears during the brain stereotactic positioning process, the negative pressure aspirator is turned on and the pressure is adjusted. Negative pressure will be generated in the residual blood collection bottle, and the generated negative pressure can attract the residual blood into the collection bottle. When the liquid in the residual blood collection bottle reaches a certain height, the stop valve on the top cover of the collection bottle automatically opens to stop the residual blood from continuing to flow into the collection bottle, thereby effectively preventing the residual blood from overflowing from the collection bottle and contaminating the operating table.

[0023] 5. This device is equipped with an animal heart rate collector. One end of the collector is connected to the power supply through a power cord. A collector start switch, a data acquisition line and a data reading display screen are designed. One end of the data line is designed to be able to fit the module of the animal's skin and contact the skin at the heart of the animal. During the stereotaxic process, press the start switch to start the heart rate collector. The real-time heart rate of the animal will be transmitted to the collector through the data line and displayed on the display screen, making it convenient for the operator to observe the value. This design enables the operator to monitor the animal's status in real time throughout the process of animal stereotaxic, effectively avoiding the death caused by the failure to timely rescue the animal.

[0024] 6. The experimental support mechanism of the device includes a support frame, a spring chain and a herringbone skin support frame, wherein one end of the spring chain is movably fixed to the upper part of the support plate by a screw, and the lower part is hinged with the herringbone skin support frame, which can flexibly control the experimental angle during the experiment to prevent the experimental mouse from struggling excessively and affecting the experimental progress.

[0025] 7. The herringbone skin support frame of the device is used to hold open the animal skin after it is cut. The herringbone skin support frame includes two symmetrical herringbone support plates. The two herringbone support plates are in contact with the skin at the opening of the animal's head to play a supporting role. Screws are installed correspondingly on the two herringbone support plates. The ends of the two screws are connected to the two ends of the spring. The screws are used to adjust the tightness of the spring, thereby adjusting the opening and closing degree of the skin support frame according to the size of the skin opening on the animal's head.

[0026] 8. The present invention is scientifically and reasonably designed and easy to use. It uses a fixing mechanism and a three-dimensional coordinate positioning mechanism to fix the experimental mouse, and can accurately and effectively adjust and fix the three-dimensional coordinates. At the same time, a rotating limit plate is provided to effectively solve the problem of deviation and low accuracy in manual operation of drilling and microinjection. It is suitable for drill bits and microinjections of various specifications, and effectively improves the accuracy and efficiency of the experiment. At the same time, the fixing mechanism, the experimental supporting mechanism and the auxiliary mechanism are used to solve the problem that after the skin on the top of the head of the animal is cut during stereotaxic positioning of the brain, the skin on both sides of the opening needs to be manually stretched to both sides with tools such as tweezers to expose the skull, but the skin stretched by tools such as tweezers is not firm enough and is easy to loosen; the problem that during the stereotaxic positioning operation of the animal's brain, the head and the area below the skull of the animal are densely covered with capillaries, and when the skin on the top of the head of the animal is cut to peel off the tissue between the skin and the skull, and when the drill bit hits the capillaries in the brain area below the skull during drilling, the capillaries rupture and bleed, and the traditional hemostatic sponge or cotton ball cannot completely collect and treat the residual blood is solved; the problem that the animal's heartbeat status cannot be monitored in real time during the stereotaxic positioning of the brain, the signs of weakness of the animal cannot be discovered in time during the stereotaxic positioning of the brain, and the animal cannot be rescued in time, resulting in the death of the animal is solved BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a front view of the present invention;

[0028] Figure 2 for Figure 1 A top view of

[0029] Figure 3 for Figure 1 Schematic diagram of the enlarged structure of the herringbone skin support frame in the middle A part. DETAILED DESCRIPTION

[0030] The present invention will be further described in detail below with reference to the accompanying drawings and through specific embodiments. The following embodiments are only illustrative and not restrictive, and the protection scope of the present invention cannot be limited thereto.

[0031] A multifunctional brain stereotaxic device, such as Figure 1 , Figure 2As shown, it includes a fixing mechanism, a three-dimensional positioning mechanism and an auxiliary mechanism, wherein the fixing mechanism, the three-dimensional positioning mechanism and the auxiliary mechanism are all arranged on a base plate, the three-dimensional positioning mechanism is arranged above the fixing mechanism, a transverse slide groove 27 is opened on the base plate, and the auxiliary mechanism is slidably arranged in the transverse slide groove through a sliding block 26, and can slide to the top of the fixing mechanism and the bottom of the three-dimensional positioning mechanism.

[0032] The fixing mechanism includes a base plate 20, a limit block 19 and a limit rod 17. The two limit blocks are symmetrically arranged on the base plate, and a limit slot is provided on the limit block. The two limit rods are detachably and symmetrically arranged in the corresponding limit slots. The limit rods are fixed by screws 18 arranged on the limit blocks.

[0033] The three-dimensional positioning mechanism includes an adjustable front and rear coordinate rod 23, an adjustable left and right coordinate rod 1, and an adjustable upper and lower coordinate rod 4. The adjustable front and rear coordinate rods are arranged on a base, the adjustable front and rear coordinate rods are connected to the adjustable upper and lower coordinate rods, the adjustable upper and lower coordinate rods are connected to the adjustable left and right coordinate rods, and the adjustable left and right coordinate rods are connected to a positioning rod linkage control shaft 5. A rotating shaft 6 is installed at the bottom end of the positioning rod linkage control shaft, and a rotating limit plate 7 is coaxially and detachably installed on the rotating shaft. A plurality of unit slots 25 for placing drill bits and syringes are opened on the rotating limit plate.

[0034] The front and rear adjustment coordinate rods are arranged in the longitudinal slide grooves 24 of the base plate, and scale lines are arranged on the rod bodies of the front and rear adjustment coordinate rods. The left and right adjustment coordinate rods and the upper and lower adjustment coordinate rods are both provided with rotating shaft rods, and the rotating shaft rods are coaxially threaded in the sleeve 3 with scales. A caliper 22 is slidably sleeved on the rotating shaft rod, and a fixed shaft rod 21 with a spring is arranged on one side of the caliper. On the rotating shaft rod, a clamp 2 for locking the rotating shaft rod is arranged on the other side of the caliper.

[0035] The auxiliary mechanism includes an experimental support unit and an auxiliary unit. The experimental support mechanism is arranged on the fixing mechanism. The auxiliary mechanism is arranged on the experimental support mechanism. The experimental support mechanism includes a support frame 9, a spring chain 11 and a herringbone skin support frame 16. The support frame is arranged on the bottom plate. One end of the spring chain is movably fixed to the upper part of the support plate by a screw 10, and the lower part is hinged with the herringbone skin support frame. Figure 3 The herringbone-shaped skin support frame includes two symmetrical herringbone support plates 29, on which screws 28 are correspondingly installed, and the ends of the two screws are connected to the two ends of the spring 30.

[0036] The auxiliary unit includes a support plate 15, a negative pressure aspirator 13 and an animal heart rate collector 8. The support plate is arranged on a support frame, and the negative pressure aspirator and the animal heart rate collector are placed on the support frame. One end of the negative pressure aspirator is provided with an interface 12 for connecting a backflow hose, and the other end is connected to a residual blood collection bottle 14.

[0037] Working principle of the present invention:

[0038] The invention provides a multifunctional brain stereotaxic positioning device, which is used for brain stereotaxic positioning operation in the process of building a rat or mouse brain model. In the process of brain stereotaxic positioning of the rat or mouse, after the skin of the animal brain is manually cut open, the three-dimensional scale positioning is realized by adjusting the front and rear coordinate rods, adjusting the left and right coordinate rods, and adjusting the upper and lower coordinate rods in the three-dimensional coordinate positioning mechanism with scales on the brain stereotaxic positioning device for mice and rats with reference to the specific positioning coordinates. Specifically, a knob on one side of the axis of the coordinate rod is manually rotated, and the spring on the coordinate rod is extended and retracted and the positioning rod is linked to the control shaft, so that the coordinate rod is moved to a specific scale, and the rotating shaft is locked by a clamp arranged on the rotating shaft, and the positioning rod is driven to link the control shaft, so that a micro-injection syringe fixed by a rotating limit plate on the rotating shaft is moved to a required coordinate point, and then a hole is drilled at the positioning point with a drill bit, and finally a specific solution required for building a certain animal model is injected into the hole with the micro-injection syringe.

[0039] The present invention arranges an auxiliary mechanism on the basis of a three-dimensional positioning mechanism, and the auxiliary mechanism comprises a negative pressure aspirator and an animal heart rate collector, one end of the negative pressure aspirator is provided with an interface for connecting a backflow hose, and the other end is connected to a blood collecting bottle, after residual blood appears during the brain stereotaxic positioning process, the negative pressure aspirator is turned on, and the pressure is adjusted, negative pressure is generated in the residual blood collecting bottle, and the generated negative pressure can attract the residual blood into the collecting bottle, and when the liquid in the residual blood collecting bottle reaches a certain height, the stop valve on the top cover of the collecting bottle is automatically opened to prevent the residual blood from continuing to flow into the collecting bottle, thereby effectively preventing the residual blood from overflowing from the collecting bottle and contaminating the operating table.

[0040] At the same time, the auxiliary mechanism of the device is also equipped with an animal heart rate collector. One end of the collector is connected to the power supply through a power cord. A collector start switch, a data acquisition line and a data reading display screen are designed. One end of the data line is designed to be able to fit the module of the animal's skin and contact the skin at the heart of the animal. During the stereotaxic process, the heart rate collector is started by pressing the start switch. The real-time heart rate of the animal will be transmitted to the collector through the data line and displayed on the display screen, making it convenient for the operator to observe the value. This design enables the operator to monitor the animal's status in real time during the entire process of animal stereotaxic, effectively avoiding the death caused by the failure to timely rescue the animal.

[0041] In addition, the experimental support mechanism of the device has made innovations on the traditional support mechanism. The support mechanism includes a support frame, a spring chain and an A-shaped skin support frame, wherein one end of the spring chain is movably fixed to the upper part of the support plate by a screw, and the lower part is hinged with the A-shaped skin support frame, which can flexibly control the experimental angle during the experiment to prevent the experimental mouse from struggling excessively and affecting the experimental progress.

[0042] The device sets a support frame on the bottom plate of the fixing mechanism, and designs a herringbone support frame for propping up the cut animal skin. The herringbone skin support frame includes two symmetrical herringbone support plates, which are in contact with the skin at the opening of the animal's head to play a supporting role. Screws are correspondingly installed on the two herringbone support plates, and the ends of the two screws are connected to the two ends of the spring. The screws are used to adjust the tightness of the spring, thereby adjusting the opening and closing degree of the skin support frame according to the size of the skin opening on the animal's head.

[0043] Although the embodiments and drawings of the present invention are disclosed for illustrative purposes, those skilled in the art will appreciate that various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims. Therefore, the scope of the present invention is not limited to the contents disclosed in the embodiments and drawings.

Claims

1. A multifunctional brain stereotaxic device, characterized in that: The invention comprises a fixing mechanism, a three-dimensional positioning mechanism and an auxiliary mechanism, wherein the fixing mechanism, the three-dimensional positioning mechanism and the auxiliary mechanism are all arranged on a bottom plate, the three-dimensional positioning mechanism is arranged above the fixing mechanism, a transverse slide groove is provided on the bottom plate, the auxiliary mechanism is slidably arranged in the transverse slide groove, and can slide to the top of the fixing mechanism and the bottom of the three-dimensional positioning mechanism, the three-dimensional positioning mechanism comprises an adjusting front and rear coordinate rod, an adjusting left and right coordinate rod, and an adjusting upper and lower coordinate rod, the adjusting front and rear coordinate rod is arranged on a base, the adjusting front and rear coordinate rod is connected to the adjusting upper and lower coordinate rod, the adjusting upper and lower coordinate rod is connected to the adjusting left and right coordinate rod The left and right coordinate rods are connected to the positioning rod linkage control shaft, a rotating shaft is installed at the bottom end of the positioning rod linkage control shaft, a rotating limit plate is coaxially and detachably installed on the rotating shaft, a plurality of unit slots for placing drill bits and syringes are opened on the rotating limit plate, the front and rear coordinate rods for adjusting are arranged in the longitudinal slide grooves of the bottom plate, scale lines are arranged on the rod bodies of the front and rear coordinate rods for adjusting, the left and right coordinate rods for adjusting and the upper and lower coordinate rods for adjusting are both provided with rotating shaft rods, the rotating shaft rods are coaxially threaded in a sleeve with scales, a caliper is slidably sleeved on the rotating shaft rod, and a spring-loaded spring is arranged on one side of the caliper The fixing shaft of the spring, on the rotating shaft, a clamp for locking the rotating shaft is arranged on the other side of the caliper, the auxiliary mechanism comprises an experimental support mechanism and an auxiliary unit, the auxiliary unit is arranged on the experimental support mechanism, the experimental support mechanism comprises a support frame, a spring chain and a herringbone skin support frame, the support frame is arranged on the bottom plate, one end of the spring chain is movably fixed to the upper part of the support plate by a screw, the lower part of the spring chain is hinged with the herringbone skin support frame, the herringbone skin support frame comprises two symmetrical herringbone support plates, screws are correspondingly installed on the two herringbone support plates, and the two screws The ends of the end connecting springs, the auxiliary unit includes a support plate, a negative pressure aspirator and an animal heart rate collector, the support plate is arranged on a support frame, the negative pressure aspirator and the animal heart rate collector are placed on the support frame, one end of the negative pressure aspirator is provided with an interface for connecting a diversion hose, and the other end is connected to a residual blood collection bottle, the fixing mechanism includes a limit block and a limit rod, the two limit blocks are symmetrically arranged on the bottom plate, a limit groove is provided on the limit block, the two limit rods are detachably and symmetrically arranged in the corresponding limit grooves, and the limit rods are fixed by screws arranged on the limit blocks.

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