A device for assisting tail vein injection of C57BL / 6 mice

Abstract

The invention discloses a device for assisting tail vein injection of C57BL / 6 mice. The device comprises a bottom plate, an external cavity, an internal accommodating cavity and an adjusting plug plate. The external cavity is fixed above the bottom plate through supporting feet, the internal accommodating cavity is arranged in the external cavity, and the adjusting plug plate is arranged at the front side of the internal accommodating cavity. The device has a simple structure and is convenient to operate. The spatial position of mice in an experimental state can be quickly and efficiently restricted, and subsequent operations are convenient. The device avoids adverse effects on the experiment caused by stress reaction of mice due to directly grabbing animals for experiments to the greatest extent. In combination with the use of an infrared light source, the tail vein injection process is visualized, and no physical damage is caused to the mice or chemical reagents are caused to penetrate into the mice, thereby further ensuring the accuracy of the experimental results.

Description

Technical Field

[0001] The invention relates to the field of experimental technology, and in particular to a device for assisting tail vein injection of C57BL / 6 mice. Background Art

[0002] Experimental C57BL / 6 mice are one of the most commonly used experimental animals for in vivo experiments because of their small size, ease of feeding and handling. They are usually used for pharmacology, toxicology, tumors, immunity, biochemistry and other related experimental operations.

[0003] However, since the C57BL / 6 mice used in the experiment are highly flexible and easy to move during the experiment, this greatly increases the difficulty of the experiment. Although mice generally do not react aggressively, when the experimenters grab and inject them, they will attack the experimenters in response to external stimuli. Usually, only highly skilled experimenters can easily and smoothly complete the tail vein injection operation. This is particularly difficult for experimenters who are not skilled in experimental operation techniques, which seriously affects the progress and accuracy of the experiment. Therefore, it is urgent to simplify the relevant experimental equipment and operation requirements so that ordinary experimenters can easily complete this operation.

[0004] In addition, the tail vein of mice is very thin, and the skin of C57BL / 6 mice is black, which significantly increases the difficulty of finding the accurate injection site of the tail vein during the experiment. The traditional operation methods mainly include: the experimenter soaks the mouse tail in warm water for several minutes, or wipes it repeatedly with ethanol or xylene, or rubs it repeatedly with the thumb and index finger, etc. The main purpose is to dilate the tail blood vessels to facilitate finding the injection site. However, these methods are not only cumbersome in operation, but also cause certain physical damage to the mouse or cause some chemical reagents to penetrate into the mouse body, which has a certain impact on the experimental results. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a device for assisting tail vein injection of C57BL / 6 mice. The device has a simple structure and can adjust the size of the accommodating tube according to the body shape of the mouse to more flexibly restrict the activity of the mouse, making it convenient for experimenters to operate and solving the problem of difficult tail vein injection of mice.

[0006] In order to achieve the above technical objectives and the above technical effects, the present invention is implemented through the following technical solutions: a device for assisting tail vein injection of C57BL / 6 mice, comprising a bottom plate, an external cavity, an internal accommodating cavity and an adjusting plug plate, the external cavity is fixed above the bottom plate by supporting feet, the internal accommodating cavity is arranged in the external cavity, and the adjusting plug plate is arranged on the front side of the internal accommodating cavity; the external cavity has no front side panel and the cross section of the external cavity is square, the internal accommodating cavity includes an upper arc plate, a lower arc plate, a left arc plate and a right arc plate, adjacent arc plates are connected by a flexible accordion connecting plate, at the center of the outer side surfaces of the four arc plates A radial adjustment device is respectively provided with a side enclosure of the external cavity on the adjacent side and movably connected thereto; a rat-tail inner groove is provided on the top of the upper arc plate; a rat-tail outer groove of the same specification is provided on the top enclosure of the external cavity at a position corresponding to the rat-tail inner groove; the adjusting plug plate comprises a plug plate body, a push rod, a push plate and a sliding rod; the plug plate body is coaxially arranged with the external cavity, the push rod is coaxially connected to the outer side surface of the plug plate body, the push plate is coaxially connected to the outer end of the push rod, the sliding rod is arranged below the push rod and movably connected to the top of the push rod, the bottom end of the sliding rod is fixedly connected to the bottom plate, and a movable top support mechanism is provided in the plug plate body.

[0007] Furthermore, the radial adjustment device includes an adjusting rod and a fixing pin, and a plurality of adjusting pin holes are evenly distributed on the adjusting rod along its length direction. Through holes suitable for the adjusting rod to pass through are respectively provided at positions corresponding to the adjusting rod on the peripheral side panels of the external cavity. The outer end of the adjusting rod passes through the through hole on the side panels of the adjacent external cavity and is locked with a fixing pin.

[0008] Furthermore, the movable supporting mechanism includes four fan-shaped grooves evenly distributed around the inner circumference of the plug plate body, a fan-shaped top block arranged in each fan-shaped groove and a pin rod integrated block arranged on the outer side of the plug plate body, the inner side surface of the fan-shaped top block is connected to the inner side surface of the fan-shaped groove by several springs, the fan-shaped top block is provided with several pin holes penetrating its front and rear sides, and external pin holes adapted to the pin holes are provided at positions corresponding to the pin holes on the front side surface of the plug plate body; the pin rod integrated block is arranged between the plug plate body and the inner sliding rod, and several sub-pins adapted to the pin holes are provided at positions corresponding to the pin holes on the inner side surface of the pin rod integrated block, and the sub-pins pass through the corresponding external pin holes and the pin holes in turn and then support the inner side surface of the plug plate body to fix the fan-shaped top block in the fan-shaped groove, at this time, the springs are all in a compressed state, and a pull rod extending along the length direction of the push rod is provided on the top of the pin rod integrated block.

[0009] Furthermore, the rat tail inner groove extends along the length direction of the upper arc plate and penetrates the front side thereof, and the rat tail outer groove also extends along the length direction of the top enclosure plate of the external cavity and penetrates the front side thereof, and the width of the rat tail inner groove and the rat tail outer groove are both 0.5-0.8 cm.

[0010] Furthermore, the internal accommodating cavity extends longitudinally and a rear side surface thereof contacts an inner side surface of a rear side panel of the external cavity.

[0011] Furthermore, a sliding block extending along the length direction of the push rod is provided at the bottom, and a sliding groove is provided at the top of the sliding rod at a position corresponding to the sliding block. The sliding block is connected in the sliding groove and can move forward and backward relative to the sliding groove.

[0012] Furthermore, a breathable mesh window is provided in the center of the rear side panel of the external cavity.

[0013] Furthermore, a plurality of suction cups are evenly distributed on the bottom of the base plate.

[0014] Furthermore, the external cavity, the four arc plates constituting the internal accommodating cavity, and the flexible accordion connecting plate are all made of transparent PVC material.

[0015] Furthermore, the top surface of the bottom plate is inclined from front to back, and the angle between the top surface and the horizontal plane is 10-25 degrees.

[0016] The beneficial effects of the present invention are:

[0017] 1. The present invention discloses a device for assisting tail vein injection of C57BL / 6 mice, which has a simple structure and is easy to operate. It can quickly and efficiently limit the spatial position of mice in an experimental state, facilitate subsequent operations, and avoid adverse effects on the experiment caused by stress reactions of mice caused by directly grabbing animals for experiments to the greatest extent. Combined with the use of infrared light sources, the tail vein injection process is visualized, and no physical damage is caused to the mice or chemical reagents are infiltrated into the mice, further ensuring the accuracy of the experimental results.

[0018] 2. The radial space of the internal accommodating cavity can be freely adjusted within a large range to achieve the effect of adapting to the body shape of the mouse. It has strong universality and can ensure the limiting effect. The mouse will not move arbitrarily during the injection process to affect the operation accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic front view of a device for assisting tail vein injection in C57BL / 6 mice;

[0020] Figure 2 for Figure 1 Schematic diagram of the structure after removing the pin rod integrated block;

[0021] Figure 3 A schematic rear view of a device for assisting tail vein injection in C57BL / 6 mice;

[0022] Figure 4 A left schematic diagram of a device for assisting tail vein injection in C57BL / 6 mice;

[0023] Figure 5 A schematic top view of a device for assisting tail vein injection in C57BL / 6 mice;

[0024] Figure 6 This is a schematic diagram of the internal structure of the plug board body after the front side panel is removed;

[0025] Figure 7 Schematic diagram of the top view of the upper arc plate;

[0026] Figure 8 It is a schematic diagram of the structure of the pin rod integrated block.

[0027] Among them, 1-bottom plate, 2-external cavity, 3-internal accommodating cavity, 4-adjusting plug plate, 5-supporting foot;

[0028] 21- rat tail external wear slot, 22- breathable mesh window;

[0029] 31-upper arc plate, 32-lower arc plate, 33-left arc plate, 34-right arc plate, 35-flexible accordion connecting plate, 36-radial adjustment device;

[0030] 311- rat tail internal groove;

[0031] 361-adjusting rod, 362-fixing pin, 363-adjusting pin hole;

[0032] 41- plug plate body, 42- push rod, 43- push plate, 44- sliding rod, 45- movable supporting mechanism;

[0033] 451-fan-shaped groove, 452-fan-shaped top block, 453-pin rod integrated block, 454-spring, 455-pin hole, 456-external pin hole, 457-sub-pin rod, 458-pull rod. DETAILED DESCRIPTION

[0034] The following is combined with Figure 1-8 The preferred embodiments of the present invention are described in detail so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby making a clearer and more definite definition of the protection scope of the present invention.

[0035] In order to reduce the difficulty of operation when performing tail vein injection on C57BL / 6 mice and ensure the operation accuracy, the present embodiment discloses a device with strong universality that can assist in tail vein injection of C57BL / 6 mice, including a bottom plate 1, an external cavity 2, an internal accommodating cavity 3 and an adjustment plug plate 4, wherein the external cavity 2 is fixed above the bottom plate 1 by supporting feet 5, the internal accommodating cavity 3 is arranged in the external cavity 2 and extends along its length direction, and the adjustment plug plate 4 is arranged on the front side of the internal accommodating cavity 3.

[0036] The external cavity 2 has no front side panel and the cross-section of the external cavity is square. A breathable mesh window 22 is provided in the center of the rear side panel of the external cavity 2 to ensure smooth ventilation for the mice.

[0037] The internal accommodating chamber 3 includes an upper arc plate 31, a lower arc plate 32, a left arc plate 33 and a right arc plate 34. Adjacent arc plates are connected by a flexible accordion connecting plate 35. The flexible accordion connecting plate 35 can be opened or contracted, and can be combined with the arc plate to play the effect of a closed enclosure, and will not cause position interference to the arc plate that is radially adjusted later. A radial adjustment device 36 is provided at the center of the outer side surface of the four arc plates, which is movably connected to the side enclosure plate of the external cavity 2 on the adjacent side. Each group of radial adjustment devices 36 includes an adjustment rod 361 and a fixing pin 362. Several adjustment pin holes 363 are evenly distributed on the adjustment rod 361 along its length direction. Through holes suitable for the adjustment rod 361 to pass through are provided at positions corresponding to the adjustment rod 361 on the peripheral side enclosure plate of the external cavity 2. The outer end of the adjustment rod 361 passes through the through hole on the side enclosure plate of the adjacent external cavity 2 and is locked with the fixing pin 362 to limit the radial movement of the corresponding arc plate.

[0038] The fixing pin 362 is a relatively small part. To prevent the fixing pin 362 from being lost, a connecting rope may be provided at one end of the fixing pin 362, and the other end of the connecting rope may be fixed to the side panel of the external cavity 2 on the corresponding side to prevent it from being lost.

[0039] A rat tail inner groove 311 is provided on the top of the upper arc plate 31, and a rat tail outer groove 21 of the same specification is provided at a position corresponding to the rat tail inner groove 311 on the top enclosure of the external cavity 2; the rat tail inner groove 311 extends along the length direction of the upper arc plate 31 and penetrates the front side thereof, and the rat tail outer groove 21 also extends along the length direction of the top enclosure of the external cavity 2 and penetrates the front side thereof, and the widths of the rat tail inner groove 311 and the rat tail outer groove 21 are both 0.5-0.8 cm. During the experiment, the rat tail passes through the two grooves for the experimenter to perform tail injection operations.

[0040] The adjusting plug plate 4 includes a plug plate body 41, a push rod 42, a push plate 43 and a sliding rod 44. The plug plate body 41 is coaxially arranged with the external cavity 2, the push rod 42 is coaxially connected to the outer surface of the plug plate body 41, the push plate 43 is coaxially connected to the outer end of the push rod 42, the sliding rod 44 is arranged below the push rod 42, and a sliding block extending along the length direction of the push rod 42 is provided at the bottom of the push rod 42, and a sliding groove is provided at the top of the sliding rod 44 at a position corresponding to the sliding block. The sliding block is connected in the sliding groove and can move forward and backward relative to the sliding groove. The bottom end of the sliding rod 44 is fixedly connected to the bottom plate 1, and a movable supporting mechanism 45 is provided in the plug plate body 41.

[0041] The movable supporting mechanism 45 includes four fan-shaped grooves 451 uniformly distributed around the inside of the plug plate body 41, a fan-shaped top block 452 arranged in each fan-shaped groove 451, and a pin rod integrated block 453 arranged on the outside of the plug plate body 41. The inner side surface of the fan-shaped top block 452 is connected to the inner side surface of the fan-shaped groove 451 by a plurality of springs 454. A telescopic column with an inner diameter matching is sleeved on the outside of the spring 454. One end of the telescopic column is fixedly connected to the inner side surface of the fan-shaped top block 452, and the other end is fixedly connected to the inner side surface of the fan-shaped groove 451. The telescopic column can stabilize the radial telescopic path of the spring. The fan-shaped top block 452 is provided with a plurality of pin holes 455 (in this embodiment, in each fan-shaped top block 452) penetrating its front and rear sides. A pin hole 455 is provided on the inside of the plug plate body 41), and an external pin hole 456 adapted to it is provided at a position corresponding to the pin hole 455 on the front side surface of the plug plate body 41; the pin rod integrated block 453 is provided between the plug plate body 41 and the inner sliding rod 44 to facilitate movement, and a plurality of sub-pin rods 457 adapted to it are provided at a position corresponding to the pin hole 455 on the inner side surface of the pin rod integrated block 453. The sub-pin rods 457 pass through the corresponding external pin holes 456 and pin holes 455 in turn and then are supported on the inner side surface of the plug plate body 41 to fix the fan-shaped top block 452 in the fan-shaped groove 451. At this time, the springs 454 are all in a compressed state, and a pull rod 458 extending along the length direction of the push rod 42 is provided on the top of the pin rod integrated block 453.

[0042] The internal accommodating cavity 3 extends longitudinally and its rear side surface contacts the inner side surface of the rear side panel of the external cavity 2 to prevent mice from escaping from the gap.

[0043] Several suction cups are evenly distributed on the bottom of the base plate 1, so as to facilitate fixing the base plate 1 on the desktop for operation.

[0044] The external cavity 2, the four arc plates constituting the internal accommodating cavity 3 and the flexible accordion connecting plate 35 are all made of transparent PVC material, which is convenient for observing the state of the mouse from the outside at any time during the operation.

[0045] The top surface of the bottom plate 1 can also be set to a shape inclined from front to back, with the angle between the top surface and the horizontal plane being 10-25°, so that the external cavity and the adjustment plug plate fixed thereon are also inclined. After the mouse is placed therein, it moves backward under the action of gravity and inertia, making it more convenient to operate the adjustment plug plate 4, which can prevent the animal from escaping to a certain extent.

[0046] The specific operation process is as follows:

[0047] The operator first preliminarily and synchronously adjusts the corresponding connection positions of the fixing pins 362 in the four radial adjustment devices 36 and the adjustment pin holes 363 on the correspondingly connected adjustment rods 361 according to the size of the mouse to adjust the accommodation space to a suitable size. Preferably, the positions of the adjustment pin holes 363 inserted into the four fixing pins 362 on the adjustment rod 361 are consistent, so that the accommodation space can be proportionally scaled in the radial direction.

[0048] Then the operator holds the mouse with one hand and puts it into the front side of the internal accommodating cavity 3 with its head facing inwards. At the same time, the operator holds the mouse's tail with the other hand and sequentially passes the mouse's tail through the mouse's tail inner groove 311 and the mouse's tail outer groove 21 until it extends out of the external cavity 2 for subsequent mouse tail intravenous injection. When the mouse enters the cavity and moves backwards, the tail can also move along the mouse's tail inner groove 311 and the mouse's tail outer groove 21, which is suitable for performing related operations on mice of different sizes.

[0049] Then, the push plate 43 is manually pushed to push the push rod 42 toward the internal accommodating chamber 3 so that the plug plate body 41 can penetrate into the cavity. The plug plate body 41 that penetrates into the internal accommodating chamber 3 can further drive the mice to move to the inside of the device. When observed from the outside, when the distance between the plug plate body 41 and the rear side panel of the external cavity 2 is adapted to the length of the mouse, one hand stabilizes the position of the push plate 43 and the other hand grasps the pull rod 458 and pulls the pull rod 458 outward to complete the separation of the pin rod integrated block 453 from the plug plate body 41. After the fan-shaped top block 452 is separated from the restraint of the sub-pin rod 457, The fan-shaped groove 451 is extended out by the elastic restoring force of the spring 454, and the outer side surface of the fan-shaped top block 452 is supported on the inner side surface of the adjacent arc plate. At this time, the position of the plug plate body 41 in the internal accommodating cavity 3 is fixed and the escape path of the mouse from behind it can be blocked, thus completing the restriction of the activity space of the mouse. The mouse will not move significantly during the tail vein injection, and the operation accuracy is guaranteed. Since the plug plate body 41 is fixed in position and is difficult to push with the strength of the mouse, the operator does not need to hold it continuously, so the operator can also free both hands to better perform subsequent operations.

[0050] After fixing the position of the plug plate body 41, if the operator believes that the radial space of the internal accommodating chamber 3 can still be further adjusted after external observation, the radial depth of the four arc plates can be fine-tuned using the radial adjustment device while observing, so as to achieve the result of accurately restricting the movement of mice. During the re-adjustment process, the spring 454 is still in a compressed state, and the outer side surface of the fan-shaped top block 452 is always supported on the inner side surface of the arc plate, which can still achieve the effect of restricting the movement of mice and preventing them from escaping.

[0051] Finally, the operator uses an infrared lamp to illuminate the mouse tail, so that the dilated tail vein becomes fully and obviously exposed, thereby achieving the effect of visualizing the tail vein blood vessels. For ease of operation, the infrared light source can also be fixed on the top enclosure of the external cavity.

[0052] After the injection is completed, it is only necessary to pull the push plate 43 outward with force to make the fan-shaped top block in the plug plate body 41 slide along the arc plate to complete the separation of the plug plate body 41 from the internal accommodating chamber 3, and the mouse can be taken out after separation. Thereafter, the operator only needs to press and contract the four fan-shaped top blocks 452 inward synchronously to make the position of the pin hole 455 correspond to the position of the external pin hole 456, and then correspondingly plug and fix the pin rod integrated block 453 on the plug plate body 41 to return the plug plate body 41 to its initial state for the next experimental operation.

[0053] With this device, ordinary operators can easily insert the syringe into the tail vein of the animal, thus solving the problem of difficulty in tail vein injection in mice.

[0054] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A device for assisting tail vein injection in C57BL / 6 mice, characterized in that: It includes a bottom plate, an external cavity, an internal accommodating cavity and an adjusting plug plate, wherein the external cavity is fixed on the top of the bottom plate through supporting feet, the internal accommodating cavity is arranged in the external cavity, and the adjusting plug plate is arranged on the front side of the internal accommodating cavity; The external cavity has no front side panel and the cross section of the external cavity is square. The internal accommodating cavity includes an upper arc plate, a lower arc plate, a left arc plate and a right arc plate. Adjacent arc plates are connected by a flexible accordion connecting plate. A radial adjustment device is respectively provided at the center of the outer side surface of the four arc plates, which is movably connected to the side panel of the external cavity on the adjacent side. A rat tail inner groove is provided on the top of the upper arc plate, and a rat tail outer groove of the same specification is provided at a position corresponding to the rat tail inner groove on the top panel of the external cavity. The regulating plug plate comprises a plug plate body, a push rod, a push plate and a sliding rod. The plug plate body is coaxially arranged with the external cavity. The push rod is coaxially connected to the outer surface of the plug plate body. The push plate is coaxially connected to the outer end of the push rod. The sliding rod is arranged below the push rod and is movably connected to the top of the push rod. The bottom end of the sliding rod is fixedly connected to the bottom plate. A movable top support mechanism is arranged in the plug plate body. The movable supporting mechanism includes four fan-shaped grooves evenly distributed around the inner circumference of the plug plate body, a fan-shaped top block arranged in each fan-shaped groove and a pin rod integrated block arranged on the outer side of the plug plate body, the inner side surface of the fan-shaped top block is connected to the inner side surface of the fan-shaped groove by several springs, several pin holes penetrating the front and rear side surfaces thereof are arranged on the fan-shaped top block, and external pin holes adapted thereto are arranged at positions corresponding to the pin holes on the front side surface of the plug plate body; the pin rod integrated block is arranged between the plug plate body and the inner sliding rod, and several sub-pin rods adapted thereto are arranged at positions corresponding to the pin holes on the inner side surface of the pin rod integrated block, and the sub-pin rods pass through the corresponding external pin holes and the pin holes in turn and then are supported on the inner side surface of the plug plate body to fix the fan-shaped top block in the fan-shaped groove, at which time the springs are all in a compressed state, and a pull rod extending along the length direction of the push rod is provided on the top of the pin rod integrated block; An infrared light source is arranged on the top enclosure of the external cavity to irradiate the rat tail so as to expand and expose the rat tail vein.

2. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: The radial adjustment device includes an adjustment rod and a fixing pin. Several adjustment pin holes are evenly distributed on the adjustment rod along its length direction. Through holes suitable for the adjustment rod to pass through are respectively provided at positions corresponding to the adjustment rod on the peripheral side panels of the external cavity. The outer end of the adjustment rod passes through the through hole on the side panels of the adjacent external cavity and is locked with a fixing pin.

3. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: The rat tail inner groove extends along the length direction of the upper arc plate and penetrates its front side. The rat tail outer groove also extends along the length direction of the top enclosure of the external cavity and penetrates its front side. The width of the rat tail inner groove and the rat tail outer groove are both 0.5-0.8 cm.

4. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: The inner accommodating cavity extends longitudinally and a rear side surface thereof contacts an inner side surface of a rear side panel of the outer cavity.

5. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: A sliding block extending along the length direction of the push rod is arranged at the bottom, and a sliding groove is arranged at a position corresponding to the sliding block at the top of the sliding rod. The sliding block is connected in the sliding groove and can move forward and backward relative to the sliding groove.

6. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: A breathable mesh window is provided in the center of the rear side panel of the outer cavity.

7. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: Several suction cups are evenly distributed on the bottom of the base plate.

8. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: The external cavity, the four arc plates constituting the internal accommodating cavity and the flexible accordion connecting plate are all made of transparent PVC material.

9. A device for assisting tail vein injection in C57BL / 6 mice as claimed in claim 1, characterized in that: The top surface of the bottom plate is inclined from front to back, and the angle between the top surface and the horizontal plane is 10-25 degrees.

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