Method for extracting procyanidine A2 from litchi rind

Abstract

The invention belongs to the technical field of plant active ingredient extraction, and particularly relates to a method for extracting procyanidine A2 from litchi rind. According to the method, industrial waste litchi rind is recycled; free procyanidine in the extract is extracted by using an ethanol solution, then combined procyanidine is hydrolyzed and separated out by using sodium hydroxide with a specific concentration, then the free and combined procyanidine in the litchi rind can be extracted to the greatest extent through separation and purification by column chromatography. The methodis easy to operate and high in product yield, and the obtained procyanidine A2 can be applied to the fields of health food, cosmetics and the like and is wide in application range. The method has important significance in increasing the additional value of litchi products and promoting the sustainable development of the industry.

Description

technical field

[0001] The invention belongs to the technical field of plant active ingredient extraction. More specifically, it relates to a method for extracting proanthocyanidin A2 from litchi peel. Background technique

[0002] Litchi is a seasonal fruit that is a specialty in southern my country, and has the reputation of "Lingnan Fruit King". It matures in the high temperature season of midsummer, and it is difficult to store and keep fresh for a long time. Therefore, in addition to fresh food, litchi is mainly used in the deep processing industry. The industrialization of lychee is mainly based on canned food, fruit juice and fruit wine, and a large amount of lychee peel is discarded as a by-product, which has not been effectively developed and utilized. Litchi skin is rich in polyphenols, especially A-type proanthocyanidins, which are less sourced in nature. Among them, procyanidins A2 (Procyanidins A2, PCA2), a dimer of proanthocyanidins polymerized by epicatechin...

Examples

Embodiment 1

[0029] Embodiment 1 A method of extracting proanthocyanidin A2 from litchi peel

[0030] The method for extracting proanthocyanidin A2 from litchi bark specifically comprises the following steps:

[0031] S1. Freeze the lychee skin, put it into a 70% ethanol solution by volume, and pulverize it so that the weight-volume ratio of the lychee skin to the ethanol solution is 1:20g / ml, extract at 50°C for 3h, centrifuge, add the bottom residue X to the ethanol solution Use the same method to extract again to obtain the combined supernatant A and the bottom residue Y after two extractions, and store them at 4°C for later use;

[0032] S2, add the bottom layer residue Y obtained in step S1 under the protection of nitrogen gas to a 2mol / L sodium hydroxide solution with a mass volume ratio of 1:15g / ml, shake and hydrolyze for 4h, and adjust the pH to 2.0, 4 with hydrochloric acid with a concentration of 6mol / L. Centrifuge at 6000rpm for 10min at ℃ to separate supernatant B;

[0033] ...

Embodiment 2

[0035] Embodiment 2 A method of extracting proanthocyanidin A2 from litchi peel

[0036] The method for extracting proanthocyanidin A2 from litchi bark specifically comprises the following steps:

[0037] S1. Freeze the lychee skin, put it into a 70% ethanol solution by volume, and pulverize it so that the weight-volume ratio of the lychee skin to the ethanol solution is 1:20g / ml, extract at 50°C for 3h, centrifuge, add the bottom residue X to the ethanol solution Use the same method to extract again to obtain the combined supernatant A and the bottom residue Y after two extractions, and store them at 4°C for later use;

[0038] S2. Add the bottom layer residue Y obtained in step S1 under nitrogen protection to a 3mol / L sodium hydroxide solution with a mass volume ratio of 1:15g / ml, shake and hydrolyze for 3h, and adjust the pH to 2.0, 4 with hydrochloric acid with a concentration of 6mol / L. Centrifuge at 6000rpm for 10min at ℃ to separate supernatant B;

[0039] S3. Combine...

Embodiment 3

[0041] Embodiment 3 A method of extracting proanthocyanidin A2 from litchi peel

[0042] The method for extracting proanthocyanidin A2 from litchi bark specifically comprises the following steps:

[0043] S1. Freeze the lychee skin, put it into a 70% ethanol solution by volume and pulverize it so that the weight volume ratio of the lychee skin to the ethanol solution is 1:15g / ml, extract at 50°C for 3h, centrifuge, add the bottom residue X to the ethanol solution Use the same method to extract again to obtain the combined supernatant A and the bottom residue Y after two extractions, and store them at 4°C for later use;

[0044] S2, add the bottom layer residue Y obtained in step S1 under the protection of nitrogen gas to a 4mol / L sodium hydroxide solution with a mass volume ratio of 1:15g / ml, shake and hydrolyze for 2h, and adjust the pH to 2.0, 4 with hydrochloric acid with a concentration of 6mol / L. Centrifuge at 6000rpm for 10min at ℃ to separate supernatant B;

[0045] S...

technical field

[0001] The invention belongs to the technical field of plant active ingredient extraction. More specifically, it relates to a method for extracting proanthocyanidin A2 from litchi peel. Background technique

[0002] Litchi is a seasonal fruit that is a specialty in southern my country, and has the reputation of "Lingnan Fruit King". It matures in the high temperature season of midsummer, and it is difficult to store and keep fresh for a long time. Therefore, in addition to fresh food, litchi is mainly used in the deep processing industry. The industrialization of lychee is mainly based on canned food, fruit juice and fruit wine, and a large amount of lychee peel is discarded as a by-product, which has not been effectively developed and utilized. Litchi skin is rich in polyphenols, especially A-type proanthocyanidins, which are less sourced in nature. Among them, procyanidins A2 (Procyanidins A2, PCA2), a dimer of proanthocyanidins polymerized by epicatechin...

Examples

Embodiment 1

[0029] Embodiment 1 A method of extracting proanthocyanidin A2 from litchi peel

[0030] The method for extracting proanthocyanidin A2 from litchi bark specifically comprises the following steps:

[0031] S1. Freeze the lychee skin, put it into a 70% ethanol solution by volume, and pulverize it so that the weight-volume ratio of the lychee skin to the ethanol solution is 1:20g / ml, extract at 50°C for 3h, centrifuge, add the bottom residue X to the ethanol solution Use the same method to extract again to obtain the combined supernatant A and the bottom residue Y after two extractions, and store them at 4°C for later use;

[0032] S2, add the bottom layer residue Y obtained in step S1 under the protection of nitrogen gas to a 2mol / L sodium hydroxide solution with a mass volume ratio of 1:15g / ml, shake and hydrolyze for 4h, and adjust the pH to 2.0, 4 with hydrochloric acid with a concentration of 6mol / L. Centrifuge at 6000rpm for 10min at ℃ to separate supernatant B;

[0033] ...

Embodiment 2

[0035] Embodiment 2 A method of extracting proanthocyanidin A2 from litchi peel

[0036] The method for extracting proanthocyanidin A2 from litchi bark specifically comprises the following steps:

[0037] S1. Freeze the lychee skin, put it into a 70% ethanol solution by volume, and pulverize it so that the weight-volume ratio of the lychee skin to the ethanol solution is 1:20g / ml, extract at 50°C for 3h, centrifuge, add the bottom residue X to the ethanol solution Use the same method to extract again to obtain the combined supernatant A and the bottom residue Y after two extractions, and store them at 4°C for later use;

[0038] S2. Add the bottom layer residue Y obtained in step S1 under nitrogen protection to a 3mol / L sodium hydroxide solution with a mass volume ratio of 1:15g / ml, shake and hydrolyze for 3h, and adjust the pH to 2.0, 4 with hydrochloric acid with a concentration of 6mol / L. Centrifuge at 6000rpm for 10min at ℃ to separate supernatant B;

[0039] S3. Combine...

Embodiment 3

[0041] Embodiment 3 A method of extracting proanthocyanidin A2 from litchi peel

[0042] The method for extracting proanthocyanidin A2 from litchi bark specifically comprises the following steps:

[0043] S1. Freeze the lychee skin, put it into a 70% ethanol solution by volume and pulverize it so that the weight volume ratio of the lychee skin to the ethanol solution is 1:15g / ml, extract at 50°C for 3h, centrifuge, add the bottom residue X to the ethanol solution Use the same method to extract again to obtain the combined supernatant A and the bottom residue Y after two extractions, and store them at 4°C for later use;

[0044] S2, add the bottom layer residue Y obtained in step S1 under the protection of nitrogen gas to a 4mol / L sodium hydroxide solution with a mass volume ratio of 1:15g / ml, shake and hydrolyze for 2h, and adjust the pH to 2.0, 4 with hydrochloric acid with a concentration of 6mol / L. Centrifuge at 6000rpm for 10min at ℃ to separate supernatant B;

[0045] S...

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