A sealing device for vertical electrophoresis device

Abstract

The present invention discloses a sealing device for a vertical electrophoresis device. The sealing device comprises: a vertical electrophoresis device body, a clamping plate and a sealing base; the vertical electrophoresis device body comprises a fixing frame and a DNA molecule fragment detection cavity; the fixing frame comprises a first fixing frame and a second fixing frame; the DNA molecule fragment detection cavity is arranged between the first fixing frame and the second fixing frame; the upper surface and the lower surface of the first fixing frame are "mountain" shaped structures, forming two gaps; the first fixing frame comprises a first side surface, a second side surface and a third side surface; the first side surface is the same as the second side surface, both of which are U-shaped structures, and a clamping plate is arranged between the two ends of the U-shaped structure; the clamping plate is rotatably connected to the two ends of the U-shaped structure; the third side surface is provided with a tightening protrusion; the tightening protrusion is used to fasten the sealing base to the fixing frame. The sealing device for a vertical electrophoresis device provided by the present invention can effectively prevent the problem of gel leakage during gel preparation.

Description

Technical Field

[0001] The invention relates to the technical field of DNA molecule separation and detection equipment, in particular to a sealing device for a vertical electrophoresis device. Background Art

[0002] The vertical electrophoresis device is an instrument for separating and detecting DNA molecules. Before separating and detecting DNA molecules, agar gel is generally used as a support, and the charge effect and molecular sieve effect of DNA molecules during swimming are utilized to achieve the purpose of separating the mixture.

[0003] Agarose gel is often used as a support for separating and identifying DNA and RNA molecular mixtures. In order to increase the detection speed of the vertical electrophoresis device and to make the agarose gel more compatible with the vertical electrophoresis device, the agarose gel is usually prepared directly on the vertical electrophoresis device. Since the gel material of agarose gel is liquid, gel leakage often occurs during the gel preparation process, resulting in gel waste. Summary of the invention

[0004] The purpose of the present invention is to provide a sealing device for a vertical electrophoresis device to solve the problem of gel leakage that often occurs in the existing gel preparation process.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] A sealing device for a vertical electrophoresis device, comprising: a vertical electrophoresis device body, a clamping plate and a sealing base;

[0007] The main body of the vertical electrophoresis device includes a fixing frame and a DNA molecule fragment detection chamber; the fixing frame includes a first fixing frame and a second fixing frame; the first fixing frame and the second fixing frame have the same structure; the DNA molecule fragment detection chamber is arranged between the first fixing frame and the second fixing frame;

[0008] The upper surface and the lower surface of the first fixing frame are of a "mountain"-shaped structure, forming two gaps; the gaps are used to place the gel preparation device; the first fixing frame includes a first side surface, a second side surface and a third side surface; the first side surface is the same as the second side surface, both of which are U-shaped structures, and a clamping plate is provided between the two ends of the U-shaped structure; the clamping plate is rotatably connected to the two ends of the U-shaped structure; the third side surface is provided with a tightening protrusion; the tightening protrusion is used to tightly buckle the sealing base on the fixing frame, so that the sealing base and the gel preparation device fit tightly.

[0009] Optionally, the sealing base specifically includes: a glue making seat, a glue making sealing strip and a fixing buckle;

[0010] The fixing buckle is provided on the upper surface of the glue making seat; the fixing buckle includes a first fixing buckle and a second fixing buckle; the glue making sealing strip is provided between the first fixing buckle and the second fixing buckle, and the glue making sealing strip is provided on the glue making seat; the fixing buckle is used to clamp the vertical electrophoresis device.

[0011] Optionally, the fixing buckle includes a vertical end, a movable buckle and a tightening buckle;

[0012] The vertical end is fixed on the glue making seat, and the vertical end is rotatably connected to the movable buckle; the movable buckle is rotatably connected to the tightening buckle.

[0013] Optionally, it also includes: a pin;

[0014] The pin shaft sequentially passes through one side of the movable buckle, the vertical end and the other side of the movable buckle, so that the vertical end is rotatably connected to the movable buckle; the movable buckle rotates through the pin shaft to drive the tightening buckle to move.

[0015] Optionally, a movable groove is provided at the rotation connection between the movable buckle and the tightening buckle; the movable groove includes a first movable groove and a second movable groove;

[0016] An active protrusion is provided on the inner side of the tightening buckle, and the active protrusion includes a first active protrusion and a second active protrusion; the first active protrusion matches the first active groove, and the second active protrusion matches the second active groove; the active groove and the active protrusion are used to rotatably connect the tightening buckle and the active buckle.

[0017] Optionally, a seesaw is provided at the bottom end of the tightening buckle;

[0018] When the tightening buckle fastens the vertical electrophoresis device in a tightened state, the seesaw is parallel to the vertical end.

[0019] Optionally, the glue-making sealing strip includes a first glue-making sealing strip and a second glue-making sealing strip;

[0020] The first glue-making sealing strip and the second glue-making sealing strip are parallel to each other, the width of the first glue-making sealing strip is equal to the width of the second glue-making sealing strip, and the width of the first glue-making sealing strip is greater than the width of the gel preparation device.

[0021] Optionally, it also includes: footing;

[0022] The foot is arranged at the bottom of the glue making seat, and the foot is used to support and fix the sealing base.

[0023] According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects: the present invention provides a sealing device for a vertical electrophoresis device, which completely seals the side and bottom surfaces of the gel preparation device through a clamping plate and a sealing base, thereby more effectively preventing the problem of gel leakage during the gel preparation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0025] Figure 1 A front view of a sealing device for a vertical electrophoresis device provided by the present invention;

[0026] Figure 2 A top view of a sealing device for a vertical electrophoresis device provided by the present invention;

[0027] Figure 3 A cross-sectional view of the sealing device of the vertical electrophoresis device provided by the present invention on the DD plane;

[0028] Figure 4 A three-dimensional diagram of the vertical electrophoresis device provided by the present invention in a clamping state of the clamping plate;

[0029] Figure 5 This is a front view of the sealing base provided by the present invention. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] The purpose of the present invention is to provide a sealing device for a vertical electrophoresis device, which can effectively prevent the problem of gel leakage during gel preparation.

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0033] Figure 1 This is a front view of the sealing device of the vertical electrophoresis device provided by the present invention, as shown Figure 1As shown, a sealing device for a vertical electrophoresis device comprises: a vertical electrophoresis device body 1, a clamping plate 2 and a sealing base 3; the vertical electrophoresis device body 1 comprises a fixing frame 4 and a DNA molecule fragment detection cavity 5; the fixing frame 4 comprises a first fixing frame and a second fixing frame; the first fixing frame and the second fixing frame have the same structure; the DNA molecule fragment detection cavity 5 is arranged between the first fixing frame and the second fixing frame; Figure 2 This is a top view of the sealing device of the vertical electrophoresis device provided by the present invention. Figure 3 This is a cross-sectional view of the sealing device DD of the vertical electrophoresis device provided by the present invention, as shown in FIG. Figure 2 - Figure 3 As shown, the upper surface and the lower surface of the first fixing frame are of a "mountain"-shaped structure, forming two gaps; the gaps are used to place the gel preparation device; the first fixing frame includes a first side surface 4-1, a second side surface 4-2 and a third side surface 4-3; the first side surface 4-1 is the same as the second side surface 4-2, both of which are U-shaped structures, and a clamping plate 2 is provided between the two ends of the U-shaped structure; the clamping plate 2 is rotatably connected to the two ends of the U-shaped structure; the third side surface 4-3 is provided with a tightening protrusion 6; the tightening protrusion 6 is used to fasten the sealing base 3 to the fixing frame 4, so that the sealing base 3 and the gel preparation device fit tightly.

[0034] The present invention also includes: an electrode plug 7 and a cross-slot pan head screw 8, wherein the electrode plug is arranged on the top of the fixing frame 4 through a washer 9, the polarity of the electrode plug 7 on the first fixing frame is positive, and the polarity of the electrode plug 7 on the second fixing frame is negative, and an electrode wire 10 is arranged in the first fixing frame and the second fixing frame, wherein the electrode wire 10 is connected to the electrode plug, and the outer surface of the electrode wire 10 is also wrapped with a platinum wire sheath 11; and the fixing frame 4 and the DNA molecular fragment detection cavity 5 are fixed together by the cross-slot pan head screw 8.

[0035] Figure 4 A three-dimensional diagram of a vertical electrophoresis device in a clamping state of the clamping plate provided by the present invention, as shown in FIG. Figure 4 As shown, according to Figure 4 It can be seen that the side leakage problem of the gel preparation device can be effectively prevented by the clamping plate 2.

[0036] Figure 5 The front view of the sealing base provided by the present invention is as follows: Figure 5 As shown, the sealing base 3 specifically includes: a glue sealing strip 3-1, a glue seat 3-2 and a fixing buckle; the fixing buckle is provided on the upper surface of the glue seat 3-2; the fixing buckle includes a first fixing buckle 3-3-1 and a second fixing buckle 3-3-2; Figure 2A top view of a sealing base for a vertical electrophoresis device provided by the present invention, such as Figure 2 As shown, the glue sealing strip 3-1 is arranged between the first fixing buckle 3-3-1 and the second fixing buckle 3-3-2, and the glue sealing strip 3-1 is arranged on the glue seat 3-2; the fixing buckle is used to clamp the vertical electrophoresis device.

[0037] In actual application, the fixed buckle includes a vertical end 3-4, a movable buckle 3-5 and a tightening buckle 3-6; the vertical end 3-4 is fixed on the glue making seat 3-2, and the vertical end 3-4 is rotatably connected to the movable buckle 3-5; the movable buckle 3-5 is rotatably connected to the tightening buckle 3-6.

[0038] In actual application, the sealing base 3 also includes: a pin shaft 3-7; the pin shaft 3-7 sequentially passes through one side of the movable buckle 3-5, the vertical end 3-4 and the other side of the movable buckle 3-5, so that the vertical end 3-4 is rotatably connected with the movable buckle 3-5; the movable buckle 3-5 rotates through the pin shaft 3-7 to drive the tightening buckle 3-6 to move.

[0039] In actual application, a movable groove is provided at the rotational connection between the movable buckle 3-5 and the tightening buckle 3-6; the movable groove includes a first movable groove 3-8-1 and a second movable groove 3-8-2; a movable protrusion is provided on the inner side of the tightening buckle 3-6, and the movable protrusion includes a first movable protrusion and a second movable protrusion; the first movable protrusion matches the first movable groove 3-8-1, and the second movable protrusion matches the second movable groove 3-8-2; the movable groove and the movable protrusion are used to rotationally connect the tightening buckle 3-6 with the movable buckle 3-5.

[0040] In actual application, a seesaw 3-9 is provided at the bottom end of the tightening buckle 3-6; when the tightening buckle 3-6 is in a tightened state when the vertical electrophoresis device is fastened, the seesaw 3-9 is parallel to the vertical end 3-4; the tightening buckle 3-6 matches the tightening protrusion 6, and the seesaw 3-9 is used to close or separate the tightening buckle 3-6 and the tightening protrusion 6; the tightening buckle 3-6 and the movable buckle 3-5 drive each other, thereby realizing the tightening or separation between the sealing base 3 and the fixing frame 4.

[0041] In actual application, the glue sealing strip 3-1 includes a first glue sealing strip and a second glue sealing strip; the first glue sealing strip and the second glue sealing strip are parallel to each other, the width of the first glue sealing strip is equal to the width of the second glue sealing strip, and the width of the first glue sealing strip is greater than the width of the gel preparation device.

[0042] The gel preparation device comprises a tall glass plate 12 and a short glass plate 13; a gel gap is provided between the tall glass plate 12 and the short glass plate 13, and the short glass plate 13 is close to the DNA molecule fragment detection cavity 5; the width of the gel gap is smaller than the width of the gel sealing strip 3-1.

[0043] A U-shaped rubber strip 14 is also provided at the bottom of the DNA molecule fragment detection chamber 5 .

[0044] The present invention also includes: a foot 3 - 11; the foot 3 - 11 is arranged at the bottom of the glue making seat 3 - 2, and the foot 3 - 11 is used to support and fix the sealing base 3.

[0045] The present invention clamps the gel preparation device by the clamping plate 2, thereby effectively preventing the gel preparation device from leaking from the side, and tightly fixes the sealing base 3 and the fixing frame 4 together by the fixing buckle in the sealing base 3, so that the gel-making sealing strip is tightly combined with the gel preparation device, effectively preventing the gel preparation device from leaking from the bottom, thereby effectively preventing the gel preparation device from leaking from the gel in all directions.

[0046] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0047] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A sealing device for a vertical electrophoresis device, characterized in that: include: A vertical electrophoresis device body, a clamping plate and a sealing base; The main body of the vertical electrophoresis device includes a fixing frame and a DNA molecule fragment detection chamber; the fixing frame includes a first fixing frame and a second fixing frame; the first fixing frame and the second fixing frame have the same structure; the DNA molecule fragment detection chamber is arranged between the first fixing frame and the second fixing frame; The upper surface and the lower surface of the first fixing frame are of a "mountain"-shaped structure, forming two gaps; the gaps are used to place the gel preparation device; the first fixing frame includes a first side surface, a second side surface and a third side surface; the first side surface is the same as the second side surface, both of which are U-shaped structures, and a clamping plate is provided between the two ends of the U-shaped structure; the clamping plate is rotatably connected to the two ends of the U-shaped structure; the third side surface is provided with a tightening protrusion; the tightening protrusion is used to fasten the sealing base to the fixing frame, so that the sealing base and the gel preparation device are tightly fitted; The sealing base specifically includes: a glue making seat, a glue making sealing strip and a fixing buckle; The fixing buckle is provided on the upper surface of the gel-making seat; the fixing buckle includes a first fixing buckle and a second fixing buckle; the gel-making sealing strip is provided between the first fixing buckle and the second fixing buckle, and the gel-making sealing strip is provided on the gel-making seat; the fixing buckle is used to clamp the vertical electrophoresis device; the gel-making sealing strip includes a first gel-making sealing strip and a second gel-making sealing strip; the first gel-making sealing strip and the second gel-making sealing strip are parallel to each other, the width of the first gel-making sealing strip is equal to the width of the second gel-making sealing strip, and the width of the first gel-making sealing strip is greater than the width of the gel preparation device; The fixed buckle includes a vertical end, a movable buckle and a tightening buckle; the vertical end is fixed on the glue making seat, and the vertical end is rotatably connected to the movable buckle; the movable buckle is rotatably connected to the tightening buckle; A movable groove is provided at the rotation connection between the movable buckle and the tightening buckle; the movable groove includes a first movable groove and a second movable groove; An active convex point is provided on the inner side of the tightening buckle, and the active convex point includes a first active convex point and a second active convex point; the first active convex point matches the first active groove, and the second active convex point matches the second active groove; the active groove and the active convex point are used to rotatably connect the tightening buckle with the active buckle; A seesaw is provided at the bottom end of the tightening buckle; when the tightening buckle is in a tightened state of fastening the vertical electrophoresis device, the seesaw is parallel to the vertical end.

2. The sealing device for a vertical electrophoresis device according to claim 1, characterized in that: Also includes: Pin; The pin shaft sequentially passes through one side of the movable buckle, the vertical end and the other side of the movable buckle, so that the vertical end is rotatably connected to the movable buckle; the movable buckle rotates through the pin shaft to drive the tightening buckle to move.

3. The sealing device for a vertical electrophoresis device according to claim 1, characterized in that: Also includes: Footings; The foot is arranged at the bottom of the glue making seat, and the foot is used to support and fix the sealing base.

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