Precise quantitative control of reagent aspiration and liquid transfer device
By designing a precise quantitative control reagent sample suction pipetting device without cross-contamination and replaceable suction heads, the cross-contamination and equipment complexity problems in the pipetting process of reagents and samples in the prior art are solved, and high-efficiency and low-loss reagents are achieved. Adding samples with improved accuracy and work efficiency of detection results.
Patent Information
- Application Number
- CN202010677518.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2020-07-15
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2040-07-15
AI Technical Summary
The existing pipetting device is prone to cross-contamination during the pipetting of reagents and samples, and the equipment is complex and not easy to miniaturize, making it difficult to achieve accurate quantitative control, affecting the accuracy of the detection results, and the suction head is replaced frequently, wastes a lot, and working efficiency. Low.
A precise quantitative control reagent sample suction pipetting device without cross-contamination and replaceable suction head is designed. It uses pipettes and droppers isolated from each other, and can realize a removable sealing connection through a pneumatic cylinder piston connecting rod and a sealing connector. To ensure that the liquid does not come into contact, use mechanical force to remove the suction head, flexibly replace it, improve working efficiency, and realize quantitative liquid suction multiple quantitative sprays through the dropper piston rod positioning controller.
The addition of reagents and samples without cross-contamination is achieved, which improves work efficiency and pipetting accuracy, reduces the use and waste of suction heads, reduces liquid residues, and improves the accuracy and production efficiency of detection results.
Smart Images
Figure CN111804357B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to a precise quantitative control reagent sample suction and liquid transfer device which is mainly used in in vitro diagnostic medical instruments and biochemical laboratory instruments such as reagent and sample liquid transfer for genetic engineering, clinical diagnostic reagent and sample liquid transfer, and industrial chemical liquid transfer, and can meet the needs of liquid surface contact sequencing and result analysis for different target sequences. Background Art
[0002] Biochemical reagents and immunochemical reagents are biological materials or organic compounds related to life science research, as well as reagents used in clinical diagnosis and medical research. There are many types of biochemical reagents, including immunochemical reagents, reagents for genetic engineering, clinical diagnostic reagents, industrial chemical reagents, additives, auxiliary reagents and other related reagents, among which immunochemical reagents include antibodies and antiserum, normal serum and complement, antigens, reagents for immunohistochemistry research, reagents for cell culture, cell separation reagents, gel diffusion method and electrophoresis reagents, etc. Reagents for genetic engineering include gene expression and gene recombination, artificially synthesized proteins, hormones, enzyme-linked immunosorbent assay kits, enzyme-linked immunosorbent assay reagents, nucleic acid synthesis reagents, nucleic acid preparations, nucleic acid detection reagents and endonucleases, etc. Clinical diagnostic reagents are mainly a large category of chemical reagents used in diagnostic examinations such as clinical pathology diagnosis, biochemical diagnosis, liquid crystal diagnosis, isotope diagnosis and general chemical diagnosis in the medical system. Industrial chemicals include industrial chemicals developed for trial production, with more than 4,000 types and increasing. Reagents are used in scientific research experiments, and the categories include: surfactants, other biological reagents, separation materials and consumables, vitamins, pigments, carbohydrates, plant hormones and nucleic acids, enzymes, antibiotics, proteins, other chemical reagents, amino acids, buffers, etc. At present, the pipetting devices of the sample loading system of automated detection and analysis equipment can be divided into needle tube type and sample suction head type. The needle tube type pipetting device can achieve high-precision pipetting function, but the main disadvantage is the possibility of cross contamination. The stainless steel needle tube must be fully cleaned between the absorption of different specimens. Most automated detection and analysis equipment uses electric pipettes to realize, which can automatically extract the sample suction head, absorb the sample or reagent through the sample suction head, and then add it to the test plate. Most of the current pipetting devices use a metering pump (or peristaltic pump, diaphragm pump) to pump liquid to achieve liquid transfer. Although a pipetting device with this structure can meet the basic needs of liquid transfer, it needs to be equipped with a metering pump for each pipette. The entire structure is relatively complicated, and the metering pump and the pipette are connected by a hose, which is not conducive to the miniaturization of the extraction device. In addition, when using a metering pump to add reagents, it is difficult to accurately control the amount of reagent added, which affects the accuracy of the subsequent test results. At the same time, it is difficult to ensure that the connection between the pipette and the liquid collection gun tip is completely sealed. If the connection between the two leaks, it will further affect the amount of reagent extracted by the metering pump.
[0003] As a means of detecting ultra-trace substances, the amount of samples and reagents used in immunoassay analysis experiments is generally in the microliter (μ) or nanoliter (n) level. Experimenters generally use manual pipettes (commonly known as pipette guns) to complete the sample and reagent filling. When there are many test items, the workload of adding samples is very huge. Therefore, there is a real need for automated equipment that can automatically complete the sample filling work for inspectors. In the process of in vitro diagnostic medical instruments and biochemical laboratory instruments, in addition to completing the reagent pipetting, sample pipetting is also required. Preparing test samples is time-consuming and laborious, and precious samples cannot withstand losses. During the reagent and sample pipetting process, no cross contamination is allowed. Therefore, in in vitro diagnostic medical instruments and biochemical laboratory instruments, pollution-free, high-precision, and high-efficiency sample and reagent addition has become a problem that plagues the entire industry. In the traditional sample and reagent addition process, two methods are mainly used: contact sample filling with replaceable pipette tips and non-contact sample filling with cleaning. Both of the above methods have the following problems and defects:
[0004] 1. Contact-type sample loading with replaceable tips
[0005] Contact-type sample addition with replaceable tips can only be applied to the first addition of samples or reagents in an empty reactor, and the tips must be discarded after adding the samples or reagents, otherwise the samples or reagents will be contaminated. There are problems and defects such as large amount of tips used, large waste, frequent tip replacement, low work efficiency, and large loss of reagents and samples.
[0006] 2. Non-contact sample addition with non-replaceable cleaning tip
[0007] The non-contact, cleaning type of sample addition can only be used in laboratory equipment where the requirements for reagent contamination are not high. Since the pipette tip cannot be replaced, there is a certain amount of residue when the pipette tip is cleaned, which is not suitable for adding test samples. Especially in in vitro diagnostic medical devices, no cross contamination is allowed, and this type of sample addition method has great limitations. Summary of the invention
[0008] The purpose of the present invention is to address the problems and shortcomings of the above-mentioned prior art and to provide a precise quantitative control pipetting device with no cross-contamination, flexible and convenient replacement of pipette tips, high working efficiency, low loss, one-time quantitative liquid aspiration and multiple quantitative liquid spraying, and replaceable pipette tips to solve the above-mentioned problems.
[0009] The present invention achieves the above-mentioned purpose through the following technical scheme: a precise quantitative control reagent sample suction pipetting device, comprising: at least one dropper pipette 16 connected to a pipette pipette 15, characterized in that: the pipette pipette 15 and the dropper pipette 16 are respectively provided with a pipette air pressure cylinder piston connecting rod 11 connected to a pipette piston rod positioning controller 13 and an air pressure container piston connecting rod 12 connected to a dropper piston rod positioning controller 14, the pipette piston cylinder 9 and the dropper piston cylinder 10 are respectively connected to the pipette 2 and the dropper 1 through a pipette pressure delivery channel 7 and a dropper pressure delivery channel 8, and the pipette 2 and the dropper 1 are respectively connected in a detachable sealed manner through a pipette sealing connector 4 and a dropper sealing connector 5 arranged on the outlet ends of the pipette pressure delivery channel 7 and the dropper pressure delivery channel 8.
[0010] Compared with the prior art, the present invention has the following beneficial effects:
[0011] No cross contamination. The present invention adopts a pipette pipette 15 and a dropper pipette 16 that are connected to each other in isolation, and a straight dropper 1 and an elbow pipette 2 that are connected to each other in an isolated common wall channel. During the pipetting process, the liquid in the replaceable pipette head has no contact with other samples, reagents, and liquids waiting for liquid transfer, thereby eliminating cross contamination between each other. Regardless of whether there are samples or reagents in the reactor, reagents and samples can be added without cross contamination, thereby completely eliminating cross contamination.
[0012] The replacement is flexible and convenient, and the work efficiency is high. The pipette 2 and the dropper 1 of the present invention are connected in a detachable sealed manner by the pipette sealing connector 4 and the dropper sealing connector 5 respectively arranged on the outlet ends of the pipette pressure delivery channel 7 and the dropper pressure delivery channel 8, so that the stored liquid can be accurately transferred and continuous pipetting can be completed quickly and conveniently. The flexible installation and removal of the pipette tip is convenient and quick, and the replacement is flexible. The use of mechanical force to remove the replaceable pipette tip greatly improves the flexibility and work efficiency of pipetting, reduces the amount of pipette tips used, and reduces waste.
[0013] High efficiency and low loss. The present invention is based on the piston pipetting principle. The pipette pipette 15 and the dropper pipette 16 use a pipette pneumatic cylinder piston connecting rod 11 connected to a pipette piston rod positioning controller 13 and a dropper pneumatic cylinder piston connecting rod 12 connected to a dropper piston rod positioning controller 14. The dropper piston rod positioning controller 14 controls the dropper pneumatic cylinder piston connecting rod 12 to instantly position and move to generate positive pressure, so that the liquid in the dropper 1 is continuously pipetted and sprayed out. The repeated sample addition time is short and the accuracy is high, which greatly improves the production efficiency. After spraying, the loss of samples and reagents is low, and there is almost no liquid residue, which reduces the production cost.
[0014] The present invention adopts a dropper piston rod positioning controller 14 to control the instant positioning movement of the dropper air cylinder piston connecting rod 12 to generate positive pressure, and non-contactly sprays the liquid in the dropper 1 to the liquid storage cavity 18, which can achieve one-time quantitative liquid aspiration and multiple quantitative liquid spraying. The dropper pipette 16 and the pipette pipette 15 adjust the liquid spraying amount each time in the liquid transfer process, and accurately control the quantitative control, which can achieve ultra-micro-precise non-contact liquid transfer, and improve the accuracy of liquid transfer. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is a cross-sectional schematic diagram of the device for accurately and quantitatively controlling the sample aspiration and liquid transfer of a reagent according to the present invention;
[0016] Figure 2 yes Figure 1 Schematic diagram of the liquid aspiration principle;
[0017] Figure 3 yes Figure 1 Schematic diagram of the principle of ejecting unstable liquid;
[0018] Figure 4 yes Figure 1 Schematic diagram of the liquid transfer principle;
[0019] Figure 5 yes Figure 1 Schematic diagram of the liquid spraying principle;
[0020] Figure 6 yes Figure 1 Schematic diagram of the principle of removing the suction tip;
[0021] Figure 7 It is a cross-sectional schematic diagram of another embodiment of the device for accurately and quantitatively controlling the sample aspiration and liquid transfer of reagents of the present invention;
[0022] Figure 8 It is a cross-sectional schematic diagram of another embodiment of the device for accurately and quantitatively controlling reagent sample aspiration and liquid transfer of the present invention.
[0023] In the figure: 1 dropper, 2 pipette, 3 suction and discharge transfer nozzle, 4 pipette sealing connector, 5 dropper sealing connector, 6 straight tube nozzle, 7 pipette pressure delivery channel, 8 dropper pressure delivery channel, 9 pipette piston cylinder, 10 dropper piston cylinder, 11 pipette air pressure cylinder piston connecting rod, 12 dropper air pressure cylinder piston connecting rod, 13 pipette piston rod positioning controller, 14 dropper piston rod positioning controller, 15 pipette pipette, 16 dropper pipette, 17 liquid sample or reagent to be transferred, 18 liquid storage nest, 19 liquid dispensing plate. DETAILED DESCRIPTION
[0024] See also Figure 1. In a preferred embodiment described below, a precise quantitative control reagent sample suction pipetting device comprises: at least one dropper pipette 16 connected to a pipette pipette 15, characterized in that: the pipette pipette 15 and the dropper pipette 16 are respectively provided with a pipette air cylinder piston connecting rod 11 connected to a pipette piston rod positioning controller 13 and a dropper air cylinder piston connecting rod 12 connected to a dropper piston rod positioning controller 14, the pipette piston cylinder 9 and the dropper piston cylinder 10 are respectively connected to the pipette 2 and the dropper 1 through the pipette pressure delivery channel 7 and the dropper pressure delivery channel 8, and the pipette 2 and the dropper 1 are respectively connected in a detachable sealed manner through the pipette sealing connector 4 and the dropper sealing connector 5 provided on the outlet ends of the pipette pressure delivery channel 7 and the dropper pressure delivery channel 8. Among them, the dropper 1 and the pipette 2 can also be implemented in the form of a straight-through dropper 1 and a straight-through pipette 2, or in the form of an elbow-type dropper 1 and a straight-through pipette 2, or in the form of an elbow-type dropper 1 and an elbow-type pipette 2. The dropper 1 and the pipette 2 can be connected by the above-mentioned common wall, or can be connected by non-common wall, or can be implemented by a split dropper and a pipette. The straight-through dropper 1 and the elbow-type pipette 2 connected by the above-mentioned common wall channel have a better pipetting accuracy effect.
[0025] See also Figure 2 Mechanical force is used to connect the pipette 2 and the pipette sealing connector 4, and the dropper 1 and the dropper sealing connector 5, and the pipette tip is placed in the liquid sample or reagent 17 to be transferred. The pipette piston rod positioning controller 13 controls the pipette air cylinder piston connecting rod 11 to move upward, so that the pipette piston cylinder 9 generates a quantitative negative pressure, and the liquid sample or reagent 17 to be transferred is quantitatively sucked into the pipette 2.
[0026] See also Figure 3 Move the pipetting device, remove the pipette tip from the liquid sample or reagent to be transferred 17, the pipette piston rod positioning controller 14 controls the pipette air cylinder piston connecting rod 12 to move downward instantly, the pipette piston cylinder 10 instantly generates positive pressure, and the unstable liquid in the pipette 1 is ejected. This step is not necessary.
[0027] See also Figure 4 The pipette piston rod positioning controller 13 controls the pipette air cylinder piston connecting rod 11 to move downward, and the pipette piston cylinder 9 generates positive pressure. The dropper piston rod positioning controller 14 controls the dropper air cylinder piston connecting rod 12 to move upward, and the dropper piston cylinder 10 generates negative pressure, so as to quantitatively transfer the liquid sample or reagent from the suction and discharge transfer nozzle 3 of the liquid transfer channel of the pipette 2 to the dropper 1.
[0028] See also Figure 5The pipetting device is moved to above one of the liquid storage holes 18 arranged on the liquid separation plate 19, and the dropper piston rod positioning controller 14 controls the dropper air cylinder piston connecting rod 12 to move downward instantly, and the dropper piston cylinder 10 generates pneumatic positive pressure to spray the liquid sample or reagent liquid in the dropper 1 from the straight tube nozzle 6 to the liquid storage hole 18 in a non-contact manner.
[0029] See also Figure 6 . Mechanical force is used to remove the replaceable pipette tip. The replaceable pipette tip includes a dropper 1 and a pipette 2 connected with a common wall. The inclined conical surface of the pipette 2 is narrowed by the same-direction inclined surface of the common-wall tube and the oppositely inclined conical surface of the suction and discharge transfer nozzle 3 to form a straight tube nozzle 6 that is interconnected. The fixed ends of the pipette 2 and the dropper 1 are respectively connected to the isolated pipette pressure delivery channel 7 and the dropper pressure delivery channel 8. The pipette connector 4 provided on the free end of the pipette pressure delivery channel 7 is connected to the pipette 2 by a detachable sealing connection. The dropper sealing connector 5 provided on the free end of the dropper pressure delivery channel 8 is connected to the dropper 1 by a detachable sealing connection.
[0030] See also Figure 7 In an optional embodiment, to facilitate the installation of structural parts during productization, the dropper piston cylinder 10 and the pipette piston cylinder 9 are respectively connected to the dropper sealing connector 5 and the pipette connector 4 in a detachable sealed connection to connect the dropper 1 and the pipette 2.
[0031] See also Figure 8 In an optional embodiment, a pipette piston rod positioning controller 13 and a dropper piston rod positioning controller 14 are provided to directly penetrate the pipette pneumatic cylinder piston connecting rod 11 and the dropper pneumatic cylinder piston connecting rod 12 into a pipette pipette 15 and a dropper pipette 16 with a sealing connector at the tail end, and the pipette pipette 15 and the dropper pipette 16 are respectively connected to the pipette piston cylinder 9 and the dropper piston cylinder 10 in one piece, and the pipette piston cylinder 9 and the dropper piston cylinder 10 are connected to the pipette 2 in a detachable sealed connection manner through the sealing connector, and the connection to the dropper 1 is realized.
[0032] The above is a preferred embodiment of the present invention. It should be noted that the above embodiment is for illustration of the present invention, but the present invention is not limited thereto, and those skilled in the art may design alternative embodiments without departing from the scope of the appended claims. For those skilled in the art, various other corresponding changes and deformations may be made according to the technical solutions and concepts described above, and all of these changes and deformations should fall within the scope of protection of the claims of the present invention.
Claims
1. A precise quantitative control reagent sample aspiration and liquid transfer device, comprising: A dropper pipette (16) having at least one connected pipette pipette (15), characterized in that: a dropper (1) and a pipette (2) connected by a common wall constitute a replaceable pipette tip, the inclined conical surface of the pipette (2) forms a mutually connected straight tube nozzle (6) through a contraction of the same-direction inclined surface of the common-wall tube and the reversely inclined conical surface of the suction and discharge transfer nozzle (3), the fixed ends of the pipette (2) and the dropper (1) are respectively connected to an isolated pipette pressure delivery channel (7) and a dropper pressure delivery channel (8), the pipette connector (4) provided on the free end of the pipette pressure delivery channel (7) is connected to the pipette (2) by a detachable sealed connection, and the dropper sealing connector (5) provided on the free end of the dropper pressure delivery channel (8) is connected to the pipette (2) by a detachable sealed connection. The pipette (1) is connected to the dropper (1) in a detachable sealed connection manner; the pipette pipette (15) and the dropper pipette (16) are respectively provided with a pipette air pressure container piston connecting rod (11) connected to a pipette piston rod positioning controller (13) and an air pressure container piston connecting rod (12) connected to a dropper piston rod positioning controller (14); the pipette piston cylinder (9) and the dropper piston cylinder (10) are respectively connected to the pipette (2) and the dropper (1) through a pipette pressure delivery channel (7) and a dropper pressure delivery channel (8); the pipette (2) and the dropper (1) are respectively connected in a detachable sealed manner through a pipette sealing connector (4) and a dropper sealing connector (5) provided on the outlet ends of the pipette pressure delivery channel (7) and the dropper pressure delivery channel (8).
2. The precise quantitative control reagent sample aspiration and liquid transfer device according to claim 1, characterized in that: Mechanical force is used to connect a pipette (2) and a pipette sealing connector (4), and to connect a dropper (1) and a dropper sealing connector (5), and a pipette tip is placed in a liquid sample or reagent (17) to be transferred. A pipette pneumatic container piston rod positioner (13) controls the pipette pneumatic connecting rod (11) to move upward, so that a pipette piston cylinder (9) generates a quantitative negative pressure, and the liquid sample or reagent (17) to be transferred is quantitatively sucked into the pipette (2).
3. The precise quantitative control reagent sample aspiration and liquid transfer device according to claim 2, characterized in that: The dropper piston rod positioning controller (14) controls the dropper air pressure container piston connecting rod (12) to move downward in an instant, and the dropper piston cylinder (10) instantly generates positive pressure to spray out the unstable liquid in the dropper (1).
4. The precise quantitative control reagent sample aspiration and liquid transfer device according to claim 3, characterized in that: The pipette pneumatic container piston rod positioner (13) controls the pipette pneumatic connecting rod (11) to move downward, and the pipette piston cylinder (9) generates positive pressure. The dropper piston rod positioning controller (14) controls the dropper pneumatic container piston connecting rod (12) to move upward, and the dropper piston cylinder (10) generates negative pressure, so that the liquid sample or reagent is quantitatively transferred from the suction and discharge transfer nozzle (3) of the liquid transfer channel of the pipette (2) to the dropper (1).
5. The precise quantitative control reagent sample aspiration and liquid transfer device according to claim 4, characterized in that: The dropper piston rod positioning controller controls the dropper air pressure container piston connecting rod (12) to move downward in an instant, and the dropper piston cylinder (10) generates pneumatic positive pressure to spray the liquid sample or reagent liquid in the dropper (1) from the straight tube nozzle (6) to the liquid storage cavity (18) in a non-contact manner.
6. The precise quantitative control reagent sample aspiration and liquid transfer device according to claim 5, characterized in that: Use mechanical force to remove replaceable pipette tips.
7. The precise quantitative control reagent sample aspiration and liquid transfer device according to claim 1, characterized in that: The dropper (1) and the pipette (2) are implemented by a straight-through dropper (1) and an elbow-type pipette (2), or by a straight-through dropper (1) and a straight-through pipette (2), or by an elbow-type dropper (1) and a straight-through pipette (2), or by an elbow-type dropper (1) and an elbow-type pipette (2).