Automatic reagent dispensing device

Abstract

The present invention discloses an automatic reagent dispensing device, including a sterile positive pressure / negative pressure system, a working liquid injection unit, a diluent injection unit, an electric optional valve unit, a liquid separation unit, and an XY displacement stage unit; the sterile positive pressure / negative pressure system provides precise pre-pressure for the working liquid injection unit and the diluent injection unit; the working liquid injection unit and the diluent injection unit are connected to the liquid inlet of the liquid separation unit, the electric directional valve unit is arranged between the diluent injection unit and the liquid separation unit, the liquid outlet of the liquid separation unit is located above the XY displacement stage unit, the liquid separation unit can move along the Z axis, and the XY displacement stage unit can move along the X axis or Y axis. Through the optimized design and coordination of each structure, any solubility of the reagent can be generated in one step automatically, quickly, accurately and on demand, and multiple reagents can be combined in any proportion.

Description

Technical Field

[0001] The invention belongs to the technical field of reagent dispensing, and in particular relates to an automatic reagent dispensing device. Background Art

[0002] At present, the preparation of concentration gradient solutions is one of the most basic operations in biochemical experiments. In cell experiments and drug screening experiments, different concentrations of biological molecules and drugs and other reagents have great differences in cell behavior and toxicity. Therefore, when investigating the effects of different concentrations of biological molecules and drug doses on cell behavior and toxicity, it is necessary to prepare a set of target solutions with gradient concentration distribution. The traditional artificial concentration gradient preparation method requires precise calculation, weighing, and multiple solution dilutions and mixing. It requires many reagent containers and is cumbersome to operate. Moreover, the cumbersome operation steps are prone to experimental errors or even mistakes, which have a certain impact on the accuracy of the experimental results and take up a lot of time for laboratory staff. Although most of the current automated pipetting workstations provide the function of configuring concentration gradients, they usually use a continuous dilution method to obtain the required concentration gradient. This process usually uses an electric pipette tip to transfer a certain amount of solution to the next level step by step and mix it to the required concentration. The operation process is also relatively time-consuming, and the step-by-step dilution process will cause the superposition of instrument solution distribution errors, further affecting the accuracy of the experimental results. In addition, due to the air piston dispensing principle of the electric pipette tip in the automated pipetting workstation, it is difficult to dispense trace amounts of solution reagents, which makes it difficult to accurately configure any low-concentration solution in one step, greatly limiting its scope of application.

[0003] On the other hand, the study of in vitro drug combinations has become increasingly important. As a complex functional system, the occurrence and development of diseases in the human body are usually a comprehensive manifestation of multiple pathologies. Single drug intervention of specific targets cannot meet the treatment requirements of complex diseases. Optimizing multiple drug combinations in vitro and formulating drug treatment plans for individual patients have become a new trend in modern drug research and development, with broad development and application prospects. The main bottleneck facing the study of combination drugs is the lack of understanding of the complex interactions of different drugs. One of the important reasons is the huge number of drugs and their dosage combinations. If the manual operation is carried out, the huge workload often discourages a large number of potential researchers. Although some high-end automated pipetting workstations currently sold on the market have the function of configuring multiple drug combinations, their workflow is complicated and time-consuming. Each drug solution needs to be repeatedly aspirated and distributed to the designated location. These complicated operation processes further limit the demand for high throughput in drug combination experiments. In addition, in order to avoid cross-contamination, each drug solution often needs to be equipped with an independent electric pipetting unit, which not only increases the complexity of instrument control but also brings high instrument prices. It is worth noting that, also limited by the air piston dispensing principle of the electric pipette tip in the automated pipetting workstation, it is difficult to dispense trace amounts of solution reagents, which further limits its ability to generate precise drug dosage combinations, causing a non-negligible impact on the experimental results.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide an automatic reagent dispensing device, which solves the problems of difficulty in dispensing trace solution reagents, complex and time-consuming reagent dispensing workflow, and large errors in combined drug dosage.

[0006] In order to achieve the above-mentioned object, the present invention provides an automatic reagent dispensing device, including a sterile positive pressure / negative pressure system, a working liquid injection unit, a diluent injection unit, an electric option valve unit, a liquid dispensing unit, and an XY displacement stage unit;

[0007] The sterile positive pressure / negative pressure system provides precise pre-pressure for the working solution injection unit and the diluent injection unit;

[0008] The working liquid injection unit and the diluent injection unit are connected to the liquid inlet of the liquid separation unit, the electric directional valve unit is arranged between the diluent injection unit and the liquid separation unit, the liquid outlet of the liquid separation unit is located above the XY displacement stage unit, the liquid separation unit can move along the Z axis direction, and the XY displacement stage unit can move along the X axis or Y axis direction.

[0009] Furthermore, it also includes a main body and a shield assembly, wherein the shield assembly is fixed to the main body through a rotating shaft assembly;

[0010] The rotating shaft assembly includes a magnet fixing ring that rotates along with its axis. A Hall sensor is provided on the rotating shaft assembly. A change in the distance between the magnet fixing ring and the Hall sensor causes a change in the magnetic field sensed by the Hall sensor, thereby obtaining the opening and closing state of the shield assembly.

[0011] Furthermore, the working liquid injection unit includes a Z-axis lifting mechanism, a gas path manifold, a transfer adapter head and a liquid storage tube;

[0012] The gas path manifold is fixed on the Z-axis lifting mechanism and moves along the Z-axis; the adapter head is detachably mounted on the gas path manifold;

[0013] When the Z-axis lifting mechanism rises to the maximum height, the bottom of the liquid inlet needle in the adapter head is located above the opening of the liquid storage tube; when the Z-axis lifting mechanism descends to the minimum height, the adapter head is sealed and connected to the liquid storage tube;

[0014] The gas path manifold is provided with a plurality of cylindrical cavities and L-shaped gas paths, the inner wall of the cylindrical cavity is provided with an internal thread structure, and the bottom is provided with a circular vertical through hole and an outlet of the L-shaped gas path.

[0015] Further, the transfer adapter includes an adapter body, a liquid inlet needle, a first sealing ring, a second sealing ring, a third sealing ring and a fourth sealing ring;

[0016] An external threaded step structure is provided on the upper part of the adapter head body, and a first circular groove, a second circular groove and a third circular groove are respectively provided on the top of the external threaded step structure from the outside to the axis center, the first circular groove and the third circular groove are respectively used to install the first sealing ring and the second sealing ring, a breathable through hole is provided at the bottom of the second circular groove, and a through liquid inlet needle is provided at the axis center of the third circular groove, and a fourth circular groove and a fifth circular groove are respectively provided on the outer side of the lower part of the adapter head body for installing the third sealing ring and the fourth sealing ring.

[0017] Further, the liquid separation unit comprises a liquid separation head and a liquid separation head base movable along the Z axis, and the liquid separation head is detachably connected to the liquid separation head base;

[0018] The bottom of the dispensing head base is provided with lighting components such as a light emitting diode array and a light-transmitting plate;

[0019] The liquid dispensing head comprises a plurality of liquid dispensing solenoid valves and a liquid dispensing nozzle detachably connected to the liquid dispensing solenoid valves, wherein the liquid dispensing solenoid valves receive reagents from the working liquid injection unit and the diluent injection unit and transport the reagents to the XY displacement stage unit;

[0020] The liquid separation head also includes a top cover plate and a base component. The top cover plate is provided with a liquid separation solenoid valve outlet at a position corresponding to the liquid separation solenoid valve, and the base component is provided with a liquid separation nozzle outlet hole at a position corresponding to the liquid separation nozzle.

[0021] Furthermore, the bottom of the liquid separation nozzle is detachably connected to a microfluid converging liquid separation head;

[0022] The microfluidic confluence and separation head contains multiple groups of microfluidic channels, each group of microfluidic channels is provided with a separation nozzle adapter channel and a confluence microchannel, the separation nozzle adapter channel and the confluence microchannel are connected, and the reagent flow received by the separation solenoid valve passes through the separation nozzle, the separation nozzle adapter channel and the confluence microchannel for confluence and then enters the separation nozzle outlet hole.

[0023] Preferably, the confluence microchannel is a wavy curved structure, the multiple groups of confluence microchannels converge vertically at the bottom of the microfluid confluence and liquid separation head, and the width of the confluence structure is no more than 3 mm, and the channel lengths of the multiple groups of confluence microchannels are the same.

[0024] Preferably, the confluence microchannel is a wavy curved structure, the turning point of the curved structure in the confluence microchannel is a smooth arc structure, multiple groups of confluence microchannels converge at the bottom of the microfluidic confluence and separation head and the angle between the confluence outlet and the horizontal plane is 15-45 degrees, the width of the confluence outlet is not more than 3mm, and the channel lengths of the multiple groups of confluence microchannels are the same.

[0025] Further, the XY displacement stage unit comprises a stage, a Y-axis displacement component and an X-axis displacement component;

[0026] The stage is provided with a well plate device for receiving the reagents discharged after the liquid dispensing unit converges, and the stage is provided with an opening structure for grabbing and placing the well plate device around it, the width of the opening structure is 10-50mm, and the edge of the stage is provided with a spring ejector structure for fixing the well plate device;

[0027] The stage is arranged on the Y-axis displacement component and can reciprocate along the Y-axis. The Y-axis displacement component is arranged on the X-axis displacement component and can drive the stage to reciprocate along the X-axis. The Y-axis displacement component is also provided with a luminous Y-axis cover.

[0028] Preferably, the waste liquid collecting unit is used to collect the waste liquid discharged from the liquid separation unit and discharge the waste liquid, and the waste liquid collecting unit includes a waste liquid collecting tank;

[0029] The waste liquid collecting tank comprises a washing liquid chamber and a waste liquid chamber, a washing liquid inlet is provided at the bottom of the washing liquid chamber, a waste liquid outlet is provided at the bottom of the waste liquid chamber, the bottom surface of the washing liquid chamber is higher than the bottom surface of the waste liquid chamber, and a slope structure is provided on one side of the bottom of the washing liquid chamber close to the waste liquid chamber.

[0030] The present invention provides an automatic reagent dispensing device, which has the following beneficial effects:

[0031] 1. The automatic reagent dispensing device involved in the present invention is provided with a sterile positive pressure / negative pressure system, a precision pressure regulating unit, a solenoid valve group unit, a working liquid injection unit, a diluent injection unit, an electric directional valve unit, a liquid dispensing unit, an XY displacement stage unit and a waste liquid collection unit, so that the reagent is dispensed on demand through precise pre-pressing and the dispensing process does not contact the substrate to achieve non-contact dispensing, with fast dispensing speed, high precision, low pollution and meeting the requirements of dispensing in a wide range of volumes (nanoliter to milliliter); in addition, the reagent dispensing at any position can be realized by linking the XY displacement stage unit with the liquid dispensing unit, so that the entire reagent dispensing process is fully automated;

[0032] 2. The automatic reagent dispensing device of the present invention can realize the generation of any concentration of reagents or the generation of multiple reagent combinations of different proportions in any micro-wells of the orifice plate in the XY displacement stage unit in one step by arranging a detachable microfluidic confluence liquid dispensing head in the liquid dispensing unit. The XY displacement stage unit does not need to address each liquid dispensing nozzle separately, which greatly saves the time required for reagent distribution. In addition, the required reagent concentration or multiple reagent combinations of different proportions are generated in one step, which avoids the superposition of instrument solution distribution errors caused by the step-by-step dilution process, further affecting the accuracy of the experimental results.

[0033] 3. The automatic reagent dispensing device involved in the present invention, by setting an optimized detachable microfluidic confluence and liquid dispensing head in the liquid dispensing unit, multiple groups of confluence microchannels converge at the bottom of the microfluidic confluence and liquid dispensing head and the angle α between the confluence outlet and the horizontal plane is in the range of 15-45 degrees, which can not only achieve the functions in the above-mentioned beneficial effects, but also by setting the confluence outlet at the bottom of the microfluidic confluence and liquid dispensing head to present a certain angle with the horizontal plane, the impact force of the distributed reagent liquid can be effectively directed to the hole wall of the well plate, further reducing the impact and disturbance of the high-speed liquid generated during the reagent dispensing process on the 2D or 3D cultured cells in the well plate, avoiding the cells being blown out of the designated culture area, or the cells being damaged or even necrotic to varying degrees due to the extremely high fluid shear force;

[0034] 4. The automatic reagent dispensing device of the present invention, by designing the dispensing head and the dispensing head base in the dispensing unit as a detachable structure, is not only convenient for users to disassemble and maintain the dispensing head in the later stage, but also the dispensing head can be set in a variety of models according to different structural styles to give users more flexible choices and additional functions and can effectively reduce the user's later use costs;

[0035] 5. The automatic reagent dispensing device involved in the present invention, by arranging components such as a Z-axis lifting mechanism, a gas manifold, and a transfer adapter in the working liquid injection unit, allows the user to disassemble and install the reagent storage tube and perform subsequent operations such as replacing or adding reagents with only one hand. This design greatly reduces the complexity and difficulty of the user's operation of the device of the present invention and improves the user's work efficiency;

[0036] 6. The automatic liquid replacement device involved in the present invention can automatically, quickly, accurately and on demand generate any solubility of reagents in one step through the optimized design and coordination of various structures, and can realize any combination of multiple reagents in proportion, which can meet the growing needs of cell experiments, drug screening experiments and in vitro drug combination research. The present invention can be used for cell experiments, drug screening and chemotherapy sensitivity detection of tumor organoids, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a schematic diagram of the overall structure of the automatic reagent dispensing device of this specific embodiment;

[0038] Figure 2 It is a structural block diagram of the automatic reagent dispensing device of this specific embodiment;

[0039] Figure 3 is a structural exploded view of the shaft assembly of this specific embodiment;

[0040] Figure 4 is a plan view of the rotating shaft assembly of this specific embodiment;

[0041] Figure 5 This is a structural exploded view of the working liquid injection unit of this specific embodiment;

[0042] Figure 6 It is a structural schematic diagram of the gas path manifold of this specific implementation mode;

[0043] Figure 7 A bottom view of the gas manifold of this specific embodiment;

[0044] Figure 8 It is a structural exploded view of the transfer adapter of this specific implementation mode;

[0045] Fig. 9 is a top view of the transfer adapter of this specific embodiment;

[0046] Fig.10 is a central cross-sectional view of the transfer adapter of this specific embodiment;

[0047] Fig.11 It is a side view of the working liquid injection unit of this specific embodiment when it is at the maximum rising height;

[0048] Fig.12 It is a side view of the working liquid injection unit of this specific embodiment when it is at the lowest descending height;

[0049] Fig.13 It is a structural schematic diagram of the liquid separation unit of this specific embodiment;

[0050] Fig.14 This is a structural exploded view of the liquid dispensing head base of this specific embodiment;

[0051] Fig.15 This is a structural exploded view of the liquid dispensing head of this specific embodiment;

[0052] Fig.16 This is a schematic structural diagram of a microfluid converging and liquid-separating head in which the converging outlet of the converging microchannels of this specific embodiment is perpendicular to the horizontal plane;

[0053] Fig.17 This is a schematic structural diagram of a microfluid converging and liquid-separating head in which the angle between the converging outlet of the converging microchannel and the horizontal plane is 15-45 degrees in this specific embodiment;

[0054] Fig.18 A bottom view of the liquid dispensing head of this specific embodiment;

[0055] Fig.19 It is a side view of the liquid dispensing head of this specific embodiment after the microfluidic converging liquid dispensing head is installed;

[0056] Fig. 20 This is a front view of the liquid dispensing head of this specific embodiment after the microfluid converging liquid dispensing head is installed;

[0057] Fig.21 It is a structural schematic diagram of the XY displacement stage unit of this specific implementation mode;

[0058] Fig. 22 It is a structural schematic diagram of the stage of this specific implementation mode;

[0059] Fig.23 It is a structural exploded view of the Y-axis displacement assembly of this specific embodiment;

[0060] Fig.24 is a cross-sectional view of the Y-axis displacement assembly of this specific embodiment;

[0061] Fig.25 This is a schematic diagram of the connection of the waste liquid collection unit pipeline of this specific implementation method;

[0062] Fig.26 It is a schematic diagram of the structure of the waste liquid collection tank of this specific implementation method.

[0063] In the figure:

[0064] 1. Main body; 2. Transparent shield assembly; 21. Transparent shield body; 22. Shaft assembly; 221. Shaft cover; 222. Shaft gasket; 223. Shaft connecting frame; 224. Damping shaft; 225. Magnet fixing ring; 2251. Cylindrical magnet; 226. Hall sensor bracket; 227. Hall sensor; 3. Sterile positive / negative pressure system; 4. Precision pressure regulating unit; 5. Solenoid valve group unit; 6. Working fluid injection unit; 61. Z-axis lifting mechanism; 62. Gas path manifold; 621. Circular vertical through hole; 622. L-shaped gas path; 623. Cylindrical cavity; 63. Adapter head; 631. Adapter head body; 6311. First annular groove; 6312. Second annular groove; 6313 , third circular groove; 6315, ventilation hole; 6316, fourth circular groove; 6317, fifth circular groove; 632, liquid inlet needle; 633, first sealing ring; 634, second sealing ring; 635, third sealing ring; 636, fourth sealing ring; 64, liquid storage tube; 65, filter bracket; 66, online filter; 67, quick release or hand screw; 7, diluent injection unit; 8, electric selection valve unit; 9, liquid dispensing unit; 901, reagent combination area; 902, reagent dilution area; 91, liquid dispensing head; 911, top cover; 912, base component; 913, circuit board assembly; 914, circuit quick-connect female / male plug; 915, liquid dispensing solenoid valve; 9151, liquid dispensing nozzle; 9152, microfluidic 1. Body confluence liquid dispensing head; 91520, microfluidic channel; 91521, liquid dispensing nozzle adapter channel; 91522, confluence microchannel; 9153, microfluidic confluence liquid dispensing head; 91530, microfluidic channel; 91531, liquid dispensing nozzle adapter channel; 91532, confluence microchannel; 916, quick release / hand screw; 917, bottom dust cover; 9171, liquid dispensing nozzle outlet hole; 9172, cylindrical magnet; 918, left and right dust cover; 919, frosted / transparent light guide column; 92, liquid dispensing head base; 921, circuit board; 922, circuit quick-connect male / female plug; 923, light-emitting diode array; 924, bottom cover; 925, frosted / transparent light-transmitting plate; 10, XY displacement stage unit; 1 01. stage; 1010. orifice plate device; 1011. opening structure; 1012. spring ejector structure; 10121. threaded housing; 10122. spring; 10123. telescopic steel ball; 10124. groove; 102. Y-axis displacement assembly; 1021. Y-axis cover plate; 10211. long strip light-transmitting hole; 1022. light guide plate; 10221. light-emitting diode assembly; 1023. stage bracket; 10231. rectangular light-shielding part; 1024. slider; 1025. guide rail; 1026. Y-axis base; 10261. driving wheel; 10262. driving wheel; 10263. driving motor; 10264. limit sensor; 103. X-axis displacement assembly; 11. waste liquid collection unit;111, waste liquid collection tank; 1111, washing liquid inlet; 1112, waste liquid outlet; 1113, washing liquid chamber; 1114, waste liquid chamber; 1115, inclined surface structure; 112, washing liquid pump; 113, washing liquid bottle; 114, waste liquid pump; 115, waste liquid bottle. ; DETAILED DESCRIPTION

[0065] In order to enable those skilled in the art to better understand the scheme of the present invention, the present invention is further described in detail below in conjunction with specific implementation methods.

[0066] The present invention can be used for cell experiments, drug screening, and chemotherapy sensitivity detection of tumor organoids.

[0067] like Figure 1-2 As shown, an automatic reagent dispensing device includes a main body 1, a protective cover assembly, a sterile positive pressure / negative pressure system 3, a precision pressure regulating unit 4, a solenoid valve group unit 5, a working liquid injection unit 6, a diluent injection unit 7, an electric option valve unit 8, a liquid separation unit 9, an XY displacement stage unit 10 and a waste liquid collection unit 11. The shield assembly is connected to the main body through the rotating shaft assembly 22, the sterile grade positive pressure / negative pressure system 3 is connected to the precision pressure regulating unit 4 and the electromagnetic valve group unit 5 in sequence through the pneumatic pipeline, the electromagnetic valve group unit 5 is further connected to the working liquid injection unit 6 and the diluent injection unit 7 through the pneumatic pipeline, the working liquid injection unit 6 is further connected to the liquid separation unit 9 through the solution pipeline, the diluent injection unit 7 is further connected to the electric directional valve unit 8 and the liquid separation unit 9 in sequence through the solution pipeline, the liquid separation unit 9 can move up and down along the Z-axis direction, the XY displacement stage unit 10 is located below the liquid separation unit 9 and can move forward, backward, left and right along the X-axis or Y-axis direction, and the waste liquid collection unit 11 is located below the XY displacement stage unit 10. The main body 1 is provided with an action actuator, which sends instructions to the action actuator of the lower computer control system through the computer human-machine interface control mechanism. The computer human-machine interface control mechanism can control the action actuator to drive the liquid dispensing unit 9 to descend to the bottom and approach the waste liquid collection unit 11, control the positive pressure system in the sterile positive pressure / negative pressure system 3 to start working and provide the set precise pre-pressure for the working liquid injection unit 6 and the diluent injection unit 7 through the precise pressure regulating unit 4 and the electromagnetic valve group unit 5. The computer human-machine interface control mechanism and the various components of the reagent automatic dispensing device are connected through a wired or wireless network.

[0068] like Figure 3-4As shown, the shield assembly includes a shield body and a shaft assembly 22, wherein the shield body can be made of a transparent material to form a transparent shield body 21, and the shield assembly is a transparent shield assembly 2. The transparent shield body 21 of the transparent shield assembly 2 is fixed to the main body 1 through the shaft assembly 22, and the shaft assembly 22 includes a shaft cover 221, a shaft gasket 222, a shaft connecting frame 223, a damping shaft 224, a magnet fixing ring 225 and a Hall sensor bracket 226. One end of the shaft connecting frame 223 is fixedly connected to the transparent shield body 21 by screws, and the other end of the shaft connecting frame 223 is connected to the main body 1 through the damping shaft 224 and the magnet fixing ring 225. The damping shaft 224 and the magnet fixing ring 225 are arranged on the inner and outer sides of the main body 1 shell, and the magnet fixing ring 225 rotates around the axis of the damping shaft 224 and follows the rotation of the shaft connecting frame 223; the shaft connecting frame 223 and the damping shaft 224 are located on the outside of the main body 1, the shaft cover 221 covers the connection between the shaft connecting frame 223 and the main body 1, and a shaft gasket 222 is arranged between the shaft cover 221 and the shaft connecting frame 223; the magnet fixing ring 225 is located on the inner side of the main body 1 shell, and a cylindrical magnet 2251 is embedded in the magnet fixing ring 225. The Hall sensor bracket 226 surrounds the magnet fixing ring 225, and two Hall sensors 227 are arranged on the Hall sensor bracket 226, and the axes of the two Hall sensors 227 are perpendicular to each other, and the two Hall sensors 227 can sense the fully closed position and the fully open position of the transparent shield body 21; when the transparent shield body 21 is opened, the shaft connecting frame 223 drives the damping shaft 224 and the magnet fixing ring 225 to rotate, and the distance between the cylindrical magnet 2251 in the magnet fixing ring 225 and the Hall sensor 227 changes, and then the Hall sensor 227 senses the magnetic field change, and it is set that one of the Hall sensors 227 controls the pressure vessel inside the reagent automatic dispensing device to release the pressure and turn on the lighting system and other operations after sensing the magnetic field change, so that it can protect the user while facilitating the user's operation; it is set that when the transparent shield body 21 is fully opened, the other Hall sensor 227 senses the magnetic field change and informs the user through the system interface that the working liquid injection unit 6 can be replaced or added.

[0069] The working liquid injection unit 6 is used to contain the reagent mother solution required for the device to distribute the reagent and is responsible for accurately pre-pressing and introducing it into the reagent container to drive the reagent to perform subsequent distribution and other operations.

[0070] like Figure 5-12As shown, the working liquid injection unit 6 includes a Z-axis lifting mechanism 61, an air path manifold 62, an adapter head 63, a liquid storage tube 64, a filter bracket 65, an online filter 66 and a quick-release or hand-tightening screw 67. The Z-axis lifting mechanism 61 is installed on the main body 1 and is controlled to rise and fall by the action actuator. The Z-axis lifting mechanism 61 is provided with a slider connecting frame for fixing the air path manifold 62 on the Z-axis lifting mechanism 61 and performing lifting and falling movements along the Z axis. The upper part of the adapter head 63 is detachably mounted on the air path manifold 62, and the lower part of the adapter head 63 can be embedded in the upper opening of the liquid storage tube 64. The online filter 66 is detachably fixed to the air path manifold 62 by the filter bracket 65 and the quick-release or hand-tightening screw 67, which is convenient for later replacement and maintenance.

[0071] A plurality of cylindrical cavities 623 and L-shaped gas paths 622 are provided on the gas path manifold plate 62. The inner wall of the cylindrical cavity 623 is provided with an internal thread structure. A circular vertical through hole 621 is provided at the bottom center axis of the cylindrical cavity 623. The circular vertical through hole 621 is connected to the outlet of the L-shaped gas path 622. The inlet direction of the L-shaped gas path 622 is toward one side of the Z-axis lifting mechanism 61.

[0072] The adapter head 63 includes an adapter head body 631, a first sealing ring 633, a second sealing ring 634, a third sealing ring 635, a fourth sealing ring 636 and a liquid inlet needle 632 that penetrates the adapter head body. The upper part of the adapter head body 631 is provided with an external thread step structure 6318 that matches the internal thread structure of the cylindrical cavity 623. The adapter head body 631 is detachably threadedly connected to the cylindrical cavity 623 at the bottom of the gas path manifold 62. The top of the structure 6318 is provided with a first annular groove 6311, a second annular groove 6312 and a third annular groove 6313 from the outside to the axis, wherein the first annular groove 6311 and the third annular groove 6313 are used to install the first sealing ring 633 and the second sealing ring 634, the bottom of the second annular groove 6312 is provided with a ventilating through hole 6315 penetrating the adapter head body 631, and the axis of the third annular groove 6313 is provided with a through hole for penetrating the liquid inlet needle 632. The lower outer side of the adapter head body 631 is provided with a fourth annular groove 6316 and a fifth annular groove 6317 arranged in parallel for installing the third sealing ring 635 and the fourth sealing ring 636 respectively.

[0073] The distance from the center of the circular vertical through hole 621 at the bottom of the cylindrical cavity 623 to the center of the outlet of the L-shaped gas path 622 is equal to the distance from the center of the air-permeable through hole 6315 to the center of the liquid inlet needle 632. The inner diameter of the circular vertical through hole 621 is larger than the outer diameter of the liquid inlet needle 632 so that the liquid inlet needle 632 can pass through the circular vertical through hole 621.

[0074] Precision pre-pressure is introduced into the liquid storage tube 64 through the air-permeable through hole 6315 through the compact L-shaped gas path 622 in the gas path manifold 62, and no additional pneumatic pipeline is required on the periphery, which reduces the complexity of the working liquid injection unit 6 and facilitates later maintenance. The adapter head 63 and the liquid storage tube 64 adopt a detachable structure. This design facilitates the replacement of a dedicated adapter head 63 for different types of liquid storage tubes 64; the liquid storage tube 64 can be directly inserted or pulled out of the adapter head 63, and the gas-tight installation of the liquid storage tube can be completed without cumbersome rotation and tightening actions, which makes the user operation more convenient.

[0075] like Fig.11 As shown, when the Z-axis lifting mechanism 61 rises to the maximum height, the working height D from the bottom of the liquid inlet needle 632 to the main body 1 is greater than the height of the liquid storage tube 64, so that there is a larger operating space during use, which facilitates the smooth installation of the liquid storage tube 64 on the adapter head 63; Fig.12 As shown, when the Z-axis lifting mechanism 61 descends to the lowest height, the liquid storage tube 64 is installed on the adapter head 63, and the bottom of the liquid storage tube 64 just contacts the working surface of the main body 1. This design can realize the self-locking function of the liquid storage tube 64. When the precision pre-pressure is applied, the bottom of the liquid storage tube 64 is against the working surface of the main body 1 to further prevent the liquid storage tube 64 from detaching from the adapter head 63, thereby preventing the leakage of precious reagents or the outflow of reagents from causing danger to the operator.

[0076] like Figure 13-20 As shown, the liquid dispensing unit 9 is used for the dilution distribution or combined distribution of multiple reagents. The liquid dispensing unit 9 includes a detachable liquid dispensing head 91 and a liquid dispensing head base 92, which is convenient for the later disassembly and maintenance of the liquid dispensing head 91, and the liquid dispensing head 91 can be designed with multiple models of different structures, providing more flexible choices and additional functions, which can effectively reduce the later use cost; the action execution mechanism of the main body 1 drives the liquid dispensing head base 92 to perform lifting and lowering movements in the Z-axis direction.

[0077] The liquid dispensing head base 92 includes a circuit board 921, a circuit quick-connect male / female plug 922, an LED array 923, a bottom cover 924 and a light-transmitting plate 925. The light-transmitting plate can be a frosted or transparent plate that can transmit light. The circuit quick-connect male / female plug 922 is arranged above the circuit board 921, the LED array 923 is arranged below the circuit board 921, and an empty frame for installing the light-transmitting plate 925 is provided on the bottom cover 924. The light emitted by the LED array 923 can pass through the light-transmitting plate 925, so that the bottom of the liquid dispensing head base 92 has a lighting function, providing a better operating field of view for the operator.

[0078] The dispensing head 91 includes a top cover plate 911, a circuit board assembly 913 and a base component 912, which are distributed from top to bottom in matching shapes. A detachable left and right split protective cover plate 918 is provided above the top cover plate 911. The circuit board assembly 913 is trapezoidal. A circuit quick-connect female / male plug 914 is connected to the wider part of the circuit board assembly 913. A plurality of dispensing solenoid valves 915 are provided in the middle position of the narrower part of the circuit board assembly 913. The circuit quick-connect female / male plug 914 matches the circuit quick-connect male / female plug 922. A detachable dispensing nozzle 9151 is provided at the bottom of the dispensing solenoid valve 915. A detachable bottom dust cover plate 917 is provided at the lower part of the narrower part of the base component 912.

[0079] The top cover plate 911 is detachably assembled with the protective cover plate 918 above by embedding multiple cylindrical magnets 9111 on the periphery. Multiple liquid separation solenoid valve outlets 9112 are provided on the top cover plate 911 at the corresponding positions of the liquid separation solenoid valve 915. The top cover plate 911 between the liquid separation solenoid valve outlets 9112 and the cylindrical magnets 9111 is provided with the same number of light-transmitting holes 9113 as the liquid separation outlets 9112. The base component 912 is provided with multiple cylindrical cavities 9121 for accommodating the liquid separation solenoid valves 915. Screws 916 are provided on both sides of the base component 912 for fixing the liquid separation head 91 on the liquid separation head base 92. The screws 916 can be quick-release or hand-tightened screws. A liquid dispensing nozzle outlet hole 9171 is provided on the bottom dust cover 917 at a position corresponding to the liquid dispensing nozzle 9151. The bottom dust cover 917 is also provided with a plurality of cylindrical magnets 9172 to realize detachable assembly with the base component 912. A plurality of light guide columns 919 are provided on the protective cover 918 at positions corresponding to the light transmission holes 9113. The optical signals of the light guide columns 919 feed back the working status of the liquid dispensing solenoid valve 915 to the operator through the light transmission holes 9113, which is convenient for the operator's debugging and maintenance.

[0080] A detachable microfluidic converging liquid-separating head 9152 is connected to the bottom of the liquid-separating nozzle 9151. The microfluidic converging liquid-separating head 9152 contains 2-8 groups of microfluidic channels. Each group of microfluidic channels 91520 of the microfluid is provided with a liquid-separating nozzle adapter channel 91521 and a converging microchannel 91522. The liquid-separating nozzle adapter channel 91521 is used to insert the liquid-separating nozzle 9151 and the liquid-separating nozzle adapter channel 91521 is connected to the converging microchannel 91522. The converging microchannel 91522 is a wavy curved structure. The turning point of the curved structure in the converging microchannel 91522 is a smooth arc structure to reduce the resistance of the fluid in the microchannel and reduce reagent residue. Multiple groups of converging microchannels 91522 converge vertically at the bottom of the microfluidic converging and liquid dispensing head 9152, and the converging structure width W is less than or equal to 3 mm. This design allows multiple distribution reagents to converge at the bottom through the microfluidic converging and liquid dispensing head 9152, and multiple reagents can be simultaneously distributed to a single microwell in the orifice plate on the bottom stage, without the need for additional displacement action to switch the position of each microwell in the orifice plate for each liquid dispensing nozzle 9151, further reducing the reagent distribution time and improving the reagent distribution efficiency; in addition, the converging structure width W Setting it to be less than or equal to 3 mm can make the width of the converging structure at the bottom of the microfluidic confluence and separation head 9152 smaller than the diameter of the micropores in various orifice plate types such as 6, 12, 24, 48, 96 and 384, so that the microfluidic confluence and separation head 9152 can be compatible with reagent distribution operations of various orifice plate types, further reducing the cost of use. In addition, setting the channel lengths of multiple groups of confluence microchannels 91522 to be the same can make the flow resistance of the liquid path for each reagent distribution the same, thereby achieving the same minimum distribution amount of each reagent under the same precision pre-pressure.

[0081] Another optimized detachable microfluidic confluence liquid-separating head 9153 can also be connected to the bottom of the liquid-separating nozzle 9151. The microfluidic confluence liquid-separating head 9153 contains 2-8 groups of microfluidic channels. Each group of microfluidic channels is provided with a liquid-separating nozzle adapter channel 91531 and a confluence microchannel 91532. The liquid-separating nozzle adapter channel 91531 is used to insert the liquid-separating nozzle 9151 and the liquid-separating nozzle adapter channel 91531 is connected to the confluence microchannel 91532. The confluence microchannel 91523 is a wavy curved structure. The turning point of the curved structure in the confluence microchannel 91523 is a smooth arc structure. Multiple groups of confluence microchannels 91532 converge at the bottom of the microfluidic confluence liquid-separating head 9153 and the confluence outlet is parallel to the horizontal plane. The angle α ranges from 15 to 45 degrees, the width W of the converging outlet is less than or equal to 3 mm, and the channel lengths of the multiple groups of converging microchannels 91532 are the same as each other; the microfluidic converging and separating head 9153 can not only realize all the functions of the microfluidic converging and separating head 9152 described above through the above-mentioned setting, but also by setting the converging outlet at the bottom of the microfluidic converging and separating head 9153 to present a certain angle with the horizontal plane, the impact force of the distributed reagent liquid can be effectively directed to the hole wall of the well plate, further reducing the impact and disturbance of the high-speed liquid generated during the reagent distribution process on the 2D or 3D cultured cells in the well plate, avoiding the cells being blown out of the designated culture area, or the cells being damaged or even necrotic to varying degrees due to the extremely high fluid shear force.

[0082] The liquid separation nozzle 9151 at the bottom of the liquid separation head 91 is divided into a longitudinally arranged reagent combination area 901 and a transversely arranged reagent dilution area 902 according to different reagent distribution functions; when conducting a combination test of multiple drug reagents, the reagent combination area 901 can selectively insert a microfluidic confluence liquid separation head 9152 / 9153 with 3 to 8 groups of microfluidic channels according to the types of distributed reagents to perform synchronous distribution of multiple reagents; when conducting a dilution test of multiple drug reagents, the reagent dilution area 902 is inserted with a microfluidic confluence liquid separation head 9152 / 9153 with 2 groups of microfluidic channels to perform synchronous distribution of working reagents and diluents.

[0083] The XY displacement stage unit 10 is located below the liquid dispensing unit 9, so that the orifice plate device 1010 installed therein can move forward, backward, left, and right along the X-axis or Y-axis direction and be linked with the liquid dispensing unit 9, so as to realize the reagent distribution at any position and make the whole reagent distribution process fully automated.

[0084] like Figure 21-24 As shown, the XY displacement stage unit 10 includes a stage 101, a Y-axis displacement component 102 and an X-axis displacement component 103. The stage 101 is arranged on the Y-axis displacement component 102 and can reciprocate along the Y-axis. The Y-axis displacement component 102 is arranged on the X-axis displacement component 103 and can drive the stage 101 to reciprocate along the X-axis.

[0085] The stage 101 is provided with four opening structures 1011 on the surrounding edges, and the width range of the opening structure 1011 is 10-50mm, so as to leave enough space for the user to grab the orifice plate device 1010 placed on the stage 101 by hand. The stage 101 is provided with spring ejector structures 1012 symmetrically distributed on the surrounding edges on any adjacent two sides, which are used for positioning and fixing the orifice plate device 1010. The spring ejector structure 1012 includes a threaded shell 10121, a spring 10122 and a telescopic steel ball 10123. The outer side of the bottom of the threaded shell 10121 is provided with a groove 10124 of any shape such as a straight line, a cross, a hexagon or a star shape, so as to facilitate the adjustment of the clamping degree of the spring ejector structure 1012 on the orifice plate device 1010 by a screwdriver.

[0086] The Y-axis displacement assembly 102 includes a Y-axis cover plate 1021 and a Y-axis base 1026. The Y-axis cover plate 1021 is provided with a long strip light-transmitting hole 10211, and a light guide plate 1022 and a light-emitting diode assembly 10221 are provided at the bottom of the Y-axis cover plate 1021 to indicate the working state of the device. The Y-axis base 1026 is provided with a stage bracket 1023, a slider 1024, a guide rail 1025, a driven wheel 10261, a driving wheel 10262, and a drive motor. 10263 and limit sensor 10264, at least two sliders 1024 are arranged on the guide rail for installing the worktable bracket 1023, a protruding rectangular shading portion 10231 is arranged at the rear of the worktable bracket 1023, the slider 1024, the guide rail 1025, the driven wheel 10261 and the driving wheel 10262 are located on the same central axis CC, and the width Hw of the slider 1024 is smaller than the effective diameter Dw of the driven wheel 10261 and the driving wheel 10262.

[0087] Therefore, the structure of the Y-axis displacement assembly 102 is more compact and lightweight and its load moment on the X-axis displacement assembly 103 is reduced to the greatest extent, further improving the positioning precision of the XY displacement stage unit 10 .

[0088] The waste liquid collection unit 11 is arranged on the main body 1, and the waste liquid collection unit 11 is located below the XY displacement stage unit 10. The waste liquid collection unit 11 is used to collect the waste liquid discharged from the liquid separation head 91 during the device cleaning or bubble removal process and discharge it from the device. Its cleanliness is crucial to maintaining a sterile environment in the device.

[0089] like Figure 25-26 As shown, the waste liquid collection unit 11 includes a waste liquid collecting tank 111, a washing liquid pump 112, a washing liquid bottle 113, a waste liquid pump 114 and a waste liquid bottle 115. The washing liquid inlet 1111 of the waste liquid collecting tank 111 is connected to the washing liquid pump 112 and the washing liquid bottle 113 in sequence through a pipeline, and the waste liquid outlet 1112 of the waste liquid collecting tank 111 is connected to the waste liquid pump 114 and the waste liquid bottle 115 in sequence through a pipeline.

[0090] The waste liquid collection tank 111 is provided with a wash liquid chamber 1113 and a waste liquid chamber 1114. The bottom of the wash liquid chamber 1113 is connected to a vertical wash liquid inlet 1111. The bottom surface of the wash liquid chamber 1113 is higher than the bottom surface of the waste liquid chamber 1114 to achieve physical isolation between the wash liquid chamber and the waste liquid chamber. A slope structure 1115 is provided on the side of the bottom of the wash liquid chamber 1113 close to the waste liquid chamber 1114 to guide the wash liquid in the wash liquid chamber 1113 into the waste liquid chamber 1114. The bottom of the waste liquid chamber 1114 is connected to a vertical waste liquid outlet 1112. The above design provides the waste liquid collection unit 11 with an additional wash liquid flushing function on the basis of the waste liquid collection function, so that the waste liquid collection unit 11 is always kept clean and sterile to avoid contamination of the entire device system.

[0091] Specific working process of the reagent automatic dispensing device:

[0092] (1) When conducting a combination test of multiple drug reagents, the reagent combination area 901 at the bottom of the dispensing head 91 can be selectively inserted into the microfluidic converging dispensing head 9152 / 9153 of 3 to 8 groups of microfluidic channels according to the type of reagent to be distributed. The computer human-machine interface control mechanism sends instructions to the reagent automatic dispensing device of the lower computer control system, and the dispensing unit 9 descends to the bottom and approaches the waste liquid collection unit 11. The positive pressure system in the sterile positive pressure / negative pressure system 3 starts to work and provides the set precision pre-pressure to the working liquid injection unit 6 through the precision pressure regulating unit 4 and the solenoid valve group unit. Then, the dispensing solenoid valves 915 that need to distribute reagents in the reagent combination area 901 are opened in turn and the bubbles are exhausted according to the preset pipeline volume of the system. During this period, the waste liquid collection unit 11 works at the same time and starts to flush the washing liquid after all the bubbles are exhausted by the dispensing solenoid valve 915. Next, the dispensing unit 9 rises to the working liquid. The working position is above the stage 101 of the XY displacement stage unit 10, and the XY displacement stage unit 10 drives the orifice plate device 1010 in the stage 101 to move to the working position below the dispensing head 91, and designs a program for the reagent distribution work. According to the program setting, a plurality of reagent combinations in different proportions are generated in one step to any micropore of the orifice plate device 1010. After completing the reagent distribution work, the XY displacement stage unit 10 further drives the stage 101 to perform rapid reciprocating or rotating movements, so that the various reagent combinations in the orifice plate device 1010 are further mixed completely, and finally the negative pressure system in the sterile positive pressure / negative pressure system 3 starts to work and provides the set precise negative pressure to the working liquid injection unit 6 through the precise pressure regulating unit 4 and the solenoid valve group unit, so that the undistributed reagent in the pipeline flows back to the liquid storage tube 64 in the working liquid injection unit 6, further reducing the waste of reagents and saving experimental costs.

[0093] (2) When conducting a test on dilution of multiple drug reagents, the required number of microfluidic converging liquid dispensing heads 9152 / 9153 with two groups of microfluidic channels can be selectively inserted into the reagent dilution area 902 at the bottom of the liquid dispensing head 91 according to the number of types of reagents to be distributed. The computer human-machine interface control mechanism sends instructions to the reagent automatic dispensing device of the lower computer control system, and the liquid dispensing unit 9 descends to the bottom and approaches the waste liquid collection unit 11. The positive pressure system in the sterile positive pressure / negative pressure system 3 starts to work and provides the set precise pre-pressure for the working liquid injection unit 6 and the diluent injection unit 7 through the precise pressure regulating unit 4 and the solenoid valve group unit. Then, the liquid dispensing solenoid valves in the reagent dilution area 902 that need to distribute the reagents are moved to the liquid dispensing unit 91. Valves 915 are opened in sequence and the bubbles are exhausted according to the preset pipeline volume of the system. During this period, the waste liquid collection unit 11 works at the same time and starts to flush the washing liquid after all the bubbles are exhausted by the liquid separation solenoid valve 915. Next, the liquid separation unit 9 rises to the working position, that is, above the stage 101 of the XY displacement stage unit 10. The XY displacement stage unit 10 drives the orifice plate device 1010 in the stage 101 to move to the working position below the liquid separation head 91. The program for reagent distribution is designed, and any required concentration of the reagent is generated in any micropore of the orifice plate device 1010 in one step according to the program setting. The subsequent workflow is consistent with the workflow for conducting a combination test of multiple drug reagents, which will not be repeated here.

[0094] The distribution frequency of the present invention can be up to 1000 times per second. The liquid dispensing solenoid valves in the reagent combination area 901 or the reagent dilution area 902 can distribute simultaneously. By precisely adjusting the injection pressure of the sterile-grade positive pressure / negative pressure system 3 and replacing the microfluidic confluence and liquid dispensing heads 9152 / 9153 with different flow resistances, the minimum single distribution volume can reach 1-50 nanoliters, and the maximum continuous distribution volume can reach 50 milliliters. The reagent distribution accuracy and precision are controlled within 1%, thereby achieving accurate reagent distribution on a global scale and further ensuring the accuracy of reagent combination or reagent dilution.

[0095] The invention concept is described in detail using specific examples herein, and the description of the above embodiments is only used to help understand the core idea of ​​the invention. It should be pointed out that any obvious modification, equivalent substitution or other improvement made by a person of ordinary skill in the art without departing from the invention concept should be included in the protection scope of the present invention.

Claims

1. A reagent automatic dispensing device, characterized in that: Including sterile grade positive pressure / negative pressure system, working liquid injection unit, diluent injection unit, electric option valve unit, liquid separation unit, XY displacement stage unit; The sterile positive pressure / negative pressure system provides precise pre-pressure for the working solution injection unit and the diluent injection unit; The working liquid injection unit and the diluent injection unit are connected to the liquid inlet of the liquid separation unit, the electric direction selection valve unit is arranged between the diluent injection unit and the liquid separation unit, the liquid outlet of the liquid separation unit is located above the XY displacement stage unit, the liquid separation unit can move along the Z axis direction, and the XY displacement stage unit can move along the X axis or the Y axis direction; The liquid dispensing unit comprises a liquid dispensing head and a liquid dispensing head base movable along the Z axis, and the liquid dispensing head is detachably connected to the liquid dispensing head base; The bottom of the dispensing head base is provided with a light emitting diode array and a light-transmitting plate lighting assembly; The liquid dispensing head comprises a plurality of liquid dispensing solenoid valves and a liquid dispensing nozzle detachably connected to the liquid dispensing solenoid valves, wherein the liquid dispensing solenoid valves receive reagents from the working liquid injection unit and the diluent injection unit and transport the reagents to the XY displacement stage unit; The liquid dispensing head further comprises a top cover plate and a base component, wherein a liquid dispensing solenoid valve outlet is provided on the top cover plate at a position corresponding to the liquid dispensing solenoid valve, and a liquid dispensing nozzle outlet hole is provided on the base component at a position corresponding to the liquid dispensing nozzle; The bottom of the liquid separation nozzle is detachably connected to a microfluid converging liquid separation head; The microfluidic confluence and liquid separation head contains a plurality of groups of microfluidic channels, each group of the microfluidic channels is provided with a liquid separation nozzle adapter channel and a confluence microchannel, the liquid separation nozzle adapter channel and the confluence microchannel are connected, and the reagent flow received by the liquid separation solenoid valve passes through the liquid separation nozzle, the liquid separation nozzle adapter channel and the confluence microchannel for confluence and then enters the liquid separation nozzle outlet hole; The confluence microchannel is a wavy curved structure, and the multiple groups of confluence microchannels converge vertically at the bottom of the microfluid confluence and liquid separation head, or the multiple groups of confluence microchannels converge at the bottom of the microfluid confluence and liquid separation head and the angle between the confluence outlet and the horizontal plane is 15-45 degrees; the width of the confluence structure is not more than 3mm, and the channel lengths of the multiple groups of confluence microchannels are the same; It also includes a main body and a shield assembly, wherein the shield assembly is fixed to the main body through a rotating shaft assembly; The rotating shaft assembly includes a magnet fixing ring that rotates along with its axis. A Hall sensor is provided on the rotating shaft assembly. A change in the distance between the magnet fixing ring and the Hall sensor causes a change in the magnetic field sensed by the Hall sensor, thereby obtaining the opening and closing state of the shield assembly.

2. The automatic reagent dispensing device according to claim 1, characterized in that: The working liquid injection unit includes a Z-axis lifting mechanism, a gas path manifold, a transfer adapter head and a liquid storage tube; The gas path manifold is fixed on the Z-axis lifting mechanism and moves along the Z-axis; the adapter head is detachably mounted on the gas path manifold; When the Z-axis lifting mechanism rises to the maximum height, the bottom of the liquid inlet needle in the adapter head is located above the opening of the liquid storage tube; when the Z-axis lifting mechanism descends to the minimum height, the adapter head is sealed and connected to the liquid storage tube; The gas path manifold is provided with a plurality of cylindrical cavities and L-shaped gas paths, the inner wall of the cylindrical cavity is provided with an internal thread structure, and the bottom is provided with a circular vertical through hole and an outlet of the L-shaped gas path.

3. The automatic reagent dispensing device according to claim 2, characterized in that The transfer adapter includes an adapter body, a liquid inlet needle, a first sealing ring, a second sealing ring, a third sealing ring and a fourth sealing ring; An external threaded step structure is provided on the upper part of the adapter head body, and a first circular groove, a second circular groove and a third circular groove are respectively provided on the top of the external threaded step structure from the outside to the axis center, the first circular groove and the third circular groove are respectively used to install the first sealing ring and the second sealing ring, a breathable through hole is provided at the bottom of the second circular groove, and a through liquid inlet needle is provided at the axis center of the third circular groove, and a fourth circular groove and a fifth circular groove are respectively provided on the outer side of the lower part of the adapter head body for installing the third sealing ring and the fourth sealing ring.

4. The automatic reagent dispensing device according to claim 1, characterized in that: The XY displacement stage unit comprises a stage, a Y-axis displacement component and an X-axis displacement component; The stage is provided with a well plate device for receiving the reagents discharged after the liquid dispensing unit converges, and the stage is provided with an opening structure for grabbing and placing the well plate device around it, the width of the opening structure is 10-50mm, and the edge of the stage is provided with a spring ejector structure for fixing the well plate device; The stage is arranged on the Y-axis displacement component and can reciprocate along the Y-axis. The Y-axis displacement component is arranged on the X-axis displacement component and can drive the stage to reciprocate along the X-axis. The Y-axis displacement component is also provided with a luminous Y-axis cover.

5. The automatic reagent dispensing device according to claim 1, characterized in that: It also includes a waste liquid collection unit, which is used to collect the waste liquid discharged from the liquid separation unit and discharge the device, and the waste liquid collection unit includes a waste liquid collection tank; The waste liquid collecting tank comprises a washing liquid chamber and a waste liquid chamber, a washing liquid inlet is provided at the bottom of the washing liquid chamber, a waste liquid outlet is provided at the bottom of the waste liquid chamber, the bottom surface of the washing liquid chamber is higher than the bottom surface of the waste liquid chamber, and a slope structure is provided on one side of the bottom of the washing liquid chamber close to the waste liquid chamber.

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