A puncture kit for destroying and sampling cartilage in rabbit knee joint

Abstract

The present invention discloses a puncture kit for cartilage destruction and sampling of rabbit knee joint, comprising a puncture sleeve, a puncture needle and a syringe, wherein the puncture needle can be inserted into the puncture sleeve, the needle tail end of the puncture needle is fixedly sleeved with a puncture needle handle, the needle head end of the puncture needle extends into the sleeve and penetrates, the needle tail of the puncture needle is connected with a needle plug, the needle head end of the puncture needle penetrating the puncture sleeve is fixed with a fixed block, a plurality of rotating shafts are fixed on the outer circumferential wall of the fixed block, a cartilage surface destruction plate is rotatably connected to the rotating shaft, and a plurality of cartilage surface destruction spikes are fixedly connected to the cartilage surface destruction plate. The beneficial effects of the present invention are: by opening the umbrella-shaped cartilage surface destruction plate of the cartilage surface destruction spike, the cartilage surface of the knee joint can be destroyed, the destruction of the knee joint tissue and the sampling efficiency are improved, the minimally invasive operation also ensures that the damage area and the size of the sample can be controlled while avoiding contamination, and the scientificity and standardization are improved.

Description

Technical Field

[0001] The invention belongs to the field of biology and medicine technology, and in particular relates to a puncture kit for destroying and sampling rabbit knee cartilage. Background Art

[0002] The incidence of cartilage injury is quite high both in athletes and the general population. Inadvertent sprains, local violent collisions, and degeneration with age in daily life may cause cartilage injury. Sports cartilage injury is one of the most common sports diseases in sports. With the development of national fitness activities, the number of people with sports cartilage injury is increasing year by year. Improper exercise or excessive exercise can easily wear cartilage. Related studies show that the incidence of osteoarthritis in my country is 3%, and there are nearly 10 million new cartilage injury patients each year. Cartilage injury is a focus of common concern among experts at home and abroad. Research on the pathological process and clinical treatment of cartilage injury diseases is of great value. In the study of cartilage injury, people often use rabbits for experiments. The commonly used methods of cartilage destruction and sampling are mainly to destroy and sample the rabbit knee joint through surgical methods. However, the surgical method has the problems of large trauma, cumbersome steps, poor rabbit compliance, easy infection, and is affected by the operator's operating skills, poor controllability, and easy to cause rabbit death. Therefore, traditional cartilage destruction and sampling methods restrict clinical application research and transformation. Summary of the invention

[0003] The object of the present invention is to provide a puncture kit for cartilage destruction and sampling of rabbit knee joints, so as to solve the problems raised in the above background technology, such as large trauma of existing cartilage destruction and sampling, cumbersome modeling steps, poor rabbit compliance, etc. The present invention is used to puncture the most obvious joint space 0.5 cm above the attachment point of the patellar ligament of the knee of an adult New Zealand white rabbit to destroy the cartilage. The puncture kit for cartilage destruction and sampling of rabbit knee joints of the present invention can perform minimally invasive destruction and sampling of rabbit cartilage tissue, reduce trauma, avoid infection, and can automatically limit the depth, thereby improving the sampling efficiency of knee joint tissue.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a puncture kit for cartilage destruction and sampling of rabbit knee joint, comprising a puncture sleeve, a puncture needle and a syringe, the puncture needle can be inserted into the puncture sleeve, the needle tail end of the puncture needle is fixedly sleeved with a puncture needle handle, the needle head end of the puncture needle extends into the sleeve and penetrates, the needle tail of the puncture needle is connected to a needle plug, the needle head end of the puncture needle that passes through the puncture sleeve is fixed with a fixed block, a plurality of rotating shafts are fixed on the outer circumferential wall of the fixed block, a cartilage surface destruction plate is rotatably connected to the rotating shaft, a plurality of cartilage surface destruction spikes are fixedly connected to the cartilage surface destruction plate, a spring is fixedly connected between the cartilage surface destruction plate and the fixed block, a closing structure is provided between the cartilage surface destruction plate and the puncture needle, and the puncture needle can be connected to the syringe after the needle plug at the tail end is pulled out. The closing structure includes a sliding block, which is slidably connected to the improved puncture needle, and a pull rod is provided between the sliding block and multiple cartilage surface destruction plates, and the pull rods are rotatably connected to connecting shafts, and the two connecting shafts are respectively fixed on the side walls opposite to the sliding block and the cartilage surface destruction plates. A wire collection cavity is provided in the puncture needle handle, and a wire collection roller is rotatably provided in the wire collection cavity, and a pull wire is wound around the wire collection roller, one end of the pull wire passes through the hole in the wire collection cavity and is fixedly connected to the wire collection roller, and the other end of the pull wire is fixedly connected to the sliding block (16).

[0005] Preferably, the fixing block is located 3 mm from the needle end of the puncture needle.

[0006] Preferably, the length of the destruction nail puncture on the cartilage surface destruction plate is 300um.

[0007] Preferably, both the cannula and the puncture needle are provided with scale lines, the scale of the puncture cannula is 10 cm, and the scale from the tail end of the improved puncture needle to the fixed block is 11 cm.

[0008] Preferably, the cartilage surface destroying plate is an umbrella-shaped structure, and the end of the bone surface destroying plate that contacts the inner wall of the sleeve is an arc-shaped structure.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] 1. When the puncture kit for rabbit knee joint cartilage destruction and sampling is used, the puncture needle is inserted into the rabbit knee joint cavity, and the cartilage surface of the knee joint can be destroyed by opening the umbrella-shaped cartilage surface destruction plate of the cartilage surface destruction spike, thereby improving the destruction of knee joint tissue and sampling efficiency. The minimally invasive operation also ensures that contamination is avoided while achieving controllable damage area and sample size, thereby improving scientificity and standardization;

[0011] 2. Since the distance between the fixing block and the needle tip is set to 3mm and the length of the damaging nail is 300um, the depth of the puncture needle entering the subchondral bone and the thickness of the damaged cartilage are guaranteed;

[0012] 3. It is equipped with closing structure, spring and other structures, which can easily realize the opening and closing of the cartilage surface destruction plate, making it more convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic cross-sectional view of the structure of the present invention;

[0014] Figure 2 for Figure 1 A schematic diagram of the structure enlarged in the oval circle;

[0015] Figure 3 It is a cross-sectional view of the puncture needle handle structure;

[0016] Figure 4 This is a diagram showing the relationship between the puncture needle handle, the hole of the line collection gun, and the position of the puncture needle;

[0017] Figure 5 This is a diagram of the connection between the syringe and the puncture needle. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0019] Please refer to the accompanying drawings, an embodiment of the present invention provides:

[0020] like Figures 1 to 5 The puncture kit shown in the figure is used for destroying and sampling cartilage of rabbit knee joint, including puncture sleeve 3, puncture needle 2 and syringe 4. When using, first put Figure 1The puncture needle is inserted into the most obvious joint space 0.5 cm above the attachment point of the patellar ligament of an adult New Zealand white rabbit, and the needle tip is inserted into the cartilage surface of the rabbit's knee joint. The puncture sleeve 3 is fixed, and the needle tail end of the puncture needle 2 is connected to the puncture needle handle 1. The needle tip of the puncture needle 2 extends into the puncture sleeve 3 and penetrates through it. The tail end of the puncture needle 2 is connected to a needle plug 8. The needle tip of the puncture needle 2 that penetrates the puncture sleeve 3 is fixed with a fixed block (15). A plurality of rotating shafts 14 are fixed on the outer circumferential wall of the fixed block 15. A cartilage surface destroying plate 11 is rotatably connected to the rotating shaft 14. A plurality of cartilage surface destroying spikes 12 are fixedly connected to the plurality of cartilage surface destroying plates 11. A spring 13 is fixedly connected between the cartilage surface destroying plate 11 and the fixed block 15. During the insertion process of the puncture needle 2, the cartilage surface destroying plate 11 is stuck in the puncture sleeve 3 and is squeezed by the inner wall of the puncture sleeve 3, so that the spring 13 is compressed; the puncture needle 2 is continuously pushed to the puncture sleeve 3. When it is completely inserted into the puncture sleeve 3, the cartilage surface destroying plate 11 is also moved out to the puncture sleeve 3, the inner wall of the puncture sleeve 3 is squeezed, and the spring 13 pops open, so that the cartilage surface destroying plate 11 opens in an umbrella shape, and the cartilage surface destroying spikes 12 stretch and destroy the cartilage surface of the knee joint. A closing structure is provided between the cartilage surface destroying plate 11 and the puncture needle 2.

[0021] In the present invention: the fixed block 15 is located 3 mm from the needle end of the puncture needle 2; the closing structure includes a sliding block 16, which is sleeved outside the puncture needle 2 and can slide relatively, and a pull rod 10 is provided between the sliding block 16 and multiple cartilage surface destruction plates 11, and both ends of the pull rod 10 are rotatably connected with connecting shafts 9, and the two connecting shafts 9 are respectively fixed on the side walls corresponding to the sliding block 16 and the cartilage surface destruction plates 11; scale lines are provided on the puncture sleeve 3 and the improved puncture needle 2, the scale of the puncture sleeve 3 is 10 cm, and the scale from the tail end of the improved puncture needle 2 to the fixed block 15 is 11 cm; a wire collection cavity 6 is opened in the puncture needle handle 1, and a wire collection roller 7 is rotatably arranged in the wire collection cavity 6, and a pull wire 5 is wound on the wire collection roller 7, one end of the pull wire 5 passes through the hole 61 on the wire collection cavity 6 and is fixedly connected to the wire collection roller 7, and the other end of the pull wire 5 is fixedly connected to the sliding block 16. After the destruction is successful, the handle 71 of the wire collecting roller 7 is rotated. When the wire collecting roller 7 rotates, the pull wire 5 is wound, and the sliding block 16 is pulled to move on the puncture needle 2, so that the two ends of the pull rod 10 rotate around the connecting shaft (9), so that the cartilage surface destruction plate 11 shrinks in an umbrella shape, compresses the spring 13, and at the same time pulls back the puncture needle 2, so that the improved puncture needle 2 is received in the puncture sleeve 3); the cartilage surface destruction plate 11 is an umbrella-shaped surface structure, and the end of the cartilage surface destruction plate 11 that contacts the inner wall of the puncture sleeve 3 is an arc-shaped structure.

[0022] Working principle: When using, first Figure 1The puncture needle is inserted into the most obvious joint space 0.5 cm above the attachment point of the patellar ligament of an adult New Zealand white rabbit. The needle tip is inserted into the cartilage surface of the rabbit's knee joint. Since the fixing block is 3 mm away from the needle tip, the depth is naturally limited to 3 mm. The puncture sleeve 3 is fixed. During the insertion of the puncture needle 2, the cartilage surface destruction plate 11 and the inner wall of the puncture sleeve 3 are squeezed by force, so that the spring 13 is compressed. The puncture needle 2 is continuously advanced until the cartilage surface destruction plate 12 is moved out of the puncture sleeve 3. The cartilage surface destruction plate 12 is not squeezed by force, and the spring 14 pops open, so that the cartilage surface destruction plate 12 opens in an umbrella shape, and the cartilage surface destruction spike 13 is stretched to destroy the cartilage surface of the knee joint. After the destruction is successful, the puncture needle is pushed into the joint cavity, the needle plug 8 is pulled out, and the connection Figure 5 The injection head 41 of the syringe 4 is injected with physiological saline to flush the damaged cartilage tissue, and then the joint fluid is extracted, the syringe is pulled out, and the handle 71 of the wire collecting roller 7 is rotated. When the wire collecting roller 7 rotates, the pull wire 5 is wound, and the sliding block 16 is pulled to move on the puncture needle 2, so that the two ends of the pull rod 10 are rotated around the connecting shaft 9, so that the cartilage surface destruction plate 11 shrinks in an umbrella shape, compresses the spring 13, and at the same time pulls back the puncture needle 2, so that the improved puncture needle 2 retreats into the puncture sleeve 3; finally, the puncture sleeve is pulled out.

[0023] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. The term "comprising" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is therefore intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in a claim should not be regarded as limiting the claim involved.

Claims

1. A puncture kit for destroying and sampling cartilage in rabbit knee joints, comprising a puncture cannula (3) and a puncture needle (2), characterized in that: The puncture needle (2) is inserted into the puncture sleeve (3), the needle tail end of the puncture needle (2) is fixedly sleeved with a puncture needle handle (1), the needle head end of the puncture needle (2) extends into and penetrates the puncture sleeve (3), and the needle head end of the puncture needle (2) that penetrates the puncture sleeve (3) is fixed with a fixing block (15), a plurality of rotating shafts (14) are fixed on the outer circumferential wall of the fixing block (15), a cartilage surface destroying plate (11) is rotatably connected to the rotating shaft (14), a plurality of cartilage surface destroying spikes (12) are fixedly connected to the cartilage surface destroying plate (11), a spring (13) is fixedly connected between the cartilage surface destroying plate (11) and the fixing block (15), and a closing structure is provided between the cartilage surface destroying plate (11) and the puncture needle (2); The closing structure comprises a sliding block (16), the sliding block (16) is sleeved outside the puncture needle (2) and can slide relatively, a pull rod (10) is provided between the sliding block (16) and a plurality of cartilage surface destruction plates (11), both ends of the pull rod (10) are rotatably connected with a connecting shaft (9), and the two connecting shafts (9) are respectively fixed on the side walls corresponding to the sliding block (16) and the cartilage surface destruction plate (11); A wire collecting cavity (6) is provided in the puncture needle handle (1), a wire collecting roller (7) is rotatably provided in the wire collecting cavity (6), a pull wire (5) is wound around the wire collecting roller (7), one end of the pull wire (5) passes through a hole (61) on the wire collecting cavity (6) and is fixedly connected to the wire collecting roller (7), and the other end of the pull wire (5) is fixedly connected to a sliding block (16); The puncture cannula (3) and the puncture needle (2) are both provided with scale lines, the scale line of the puncture cannula (3) is 10 cm, and the scale line from the tail end of the puncture needle (2) to the fixing block (15) is 11 cm; The plurality of cartilage surface destroying plates (11) are in an umbrella-shaped structure, and the end of the cartilage surface destroying plate (11) that contacts the inner wall of the puncture sleeve (3) is in an arc-shaped structure.

2. A puncture kit for rabbit knee cartilage destruction and sampling according to claim 1, characterized in that: The fixing block (15) is located 3 mm from the needle end of the puncture needle (2).

3. A puncture kit for rabbit knee cartilage destruction and sampling according to claim 1, characterized in that: The length of the cartilage surface destroying nails on the cartilage surface destroying plate (11) is 300 um.

4. A puncture kit for rabbit knee cartilage destruction and sampling according to claim 1, characterized in that: The tail end of the puncture needle has an opening, and a needle plug (8) is inserted into the opening. After the needle plug is removed, the puncture needle can be connected to the syringe (4).

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