High-flux microbial inoculation device based on spray assistance

Abstract

The invention belongs to the field of microbiological analysis and micromanufacturing, and discloses a microbial inoculation device based on spray assistance. The microbial inoculation device sequentially comprises from top to bottom: an aerosol droplet generation device for atomizing a microbial solution containing a target microbial strain to form aerosol droplets; an aerosol liquid drop restraining and settling channel used for restraining the settling and diffusion area of the aerosol liquid drops and forming a liquid drop diffusion and settling channel so that the settling and collectingarea of the liquid drops is controlled; moreover, the tail end of the aerosol liquid drop restraining and settling channel is used for being seamlessly connected with a solid culture medium so that inoculation of target microorganisms on the solid culture medium is realized, and subsequent culture of single colonies of the target microorganisms is facilitated. By improving the arrangement of all components in the device, the corresponding matching working mode and the like, the problem that in the prior art, a microorganism single colony culture device which is easy to operate, low in equipment price and high in flux does not exist can be effectively solved.

Description

technical field

[0001] The invention belongs to the field of microbial analysis and micromanufacturing, and more specifically relates to a spray-assisted high-throughput microbial inoculation device, especially a high-throughput microbial inoculation device based on ultrasonic spray assistance, which facilitates high-throughput microbial inoculation Single colony culture. Background technique

[0002] Microorganisms are closely related to human life, and are widely used in fields such as medicine, chemical industry, agricultural products, chemical energy, and environmental protection. Traditional microbial pure culture methods include streak inoculation, smear inoculation and liquid inoculation, but these traditional inoculation methods have problems such as heavy workload, need for operating experience, and low throughput of single colony culture with good countability. For example, the most commonly used smear inoculation method, the unknown sample is serially diluted and...

Examples

Embodiment 1

[0050] In the present invention, based on the spray-assisted high-throughput microorganism inoculation device, it can be used in conjunction with solid medium when in use, that is, it is composed of four parts: a spray generating device, a settling channel, a mask assembly and a solid medium (of course, the mask assembly is also optional).

[0051] Such as figure 1 As shown, the spray device is fixed on the settling channel with screw port. In this embodiment, the ultrasonic atomizing sheet is preferably selected, and its outer diameter is 18mm. Through the fixing and sealing of the screw port of the fixing seat and the settling channel, the leakage of spray droplets is prevented.

[0052] In this embodiment, the settling channel is preferably refitted with a 500ml plastic bottle, which is low in cost and easy to process. Cut off the bottom of the plastic bottle, sand it to a level, and connect it seamlessly with the solid medium to prevent the spray aerosol droplets from l...

technical field

[0001] The invention belongs to the field of microbial analysis and micromanufacturing, and more specifically relates to a spray-assisted high-throughput microbial inoculation device, especially a high-throughput microbial inoculation device based on ultrasonic spray assistance, which facilitates high-throughput microbial inoculation Single colony culture. Background technique

[0002] Microorganisms are closely related to human life, and are widely used in fields such as medicine, chemical industry, agricultural products, chemical energy, and environmental protection. Traditional microbial pure culture methods include streak inoculation, smear inoculation and liquid inoculation, but these traditional inoculation methods have problems such as heavy workload, need for operating experience, and low throughput of single colony culture with good countability. For example, the most commonly used smear inoculation method, the unknown sample is serially diluted and...

Examples

Embodiment 1

[0050] In the present invention, based on the spray-assisted high-throughput microorganism inoculation device, it can be used in conjunction with solid medium when in use, that is, it is composed of four parts: a spray generating device, a settling channel, a mask assembly and a solid medium (of course, the mask assembly is also optional).

[0051] Such as figure 1 As shown, the spray device is fixed on the settling channel with screw port. In this embodiment, the ultrasonic atomizing sheet is preferably selected, and its outer diameter is 18mm. Through the fixing and sealing of the screw port of the fixing seat and the settling channel, the leakage of spray droplets is prevented.

[0052] In this embodiment, the settling channel is preferably refitted with a 500ml plastic bottle, which is low in cost and easy to process. Cut off the bottom of the plastic bottle, sand it to a level, and connect it seamlessly with the solid medium to prevent the spray aerosol droplets from l...

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