A spray-assisted high-throughput microbial inoculation device

An inoculation device and microbial technology, applied in the field of high-throughput microbial inoculation devices, can solve problems such as inability to meet the needs of microbial culture and detection, and achieve ultra-high-throughput microbial single-cell inoculation, pure colony culture, and increase Effect of Inoculation Flux and Uniformity of Distribution

CN112430528BActive Publication Date: 2022-05-20HUAZHONG UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2022-05-20

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Abstract

The invention belongs to the field of microbial analysis and micro-manufacturing, and discloses a spray-assisted microbial inoculation device, which includes from top to bottom: an aerosol droplet generating device for atomizing a microbial solution containing target microbial strains processing to form aerosol droplets; the aerosol droplets constrain the settling channel for confining the settling and spreading area of ​​the aerosol droplet, and form the droplet diffusion settling channel, thereby restricting the settling and collecting area of ​​the droplet control; and, the end of the aerosol droplet constraining the sedimentation channel is used to seamlessly connect with the solid medium to realize the inoculation of the target microorganism on the solid medium, so as to facilitate the subsequent cultivation of a single colony of the target microorganism. The present invention can effectively solve the problem of lack of simple operation, cheap equipment, and high-throughput microbial single colony cultivation device in the prior art by improving the arrangement of each component in the device and the corresponding cooperative working mode.
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Description

technical field

[0001] The invention belongs to the field of microbial analysis and micro-manufacturing, and more particularly relates to a high-throughput microbial inoculation device based on spray assistance, especially a high-throughput microbial inoculation device based on ultrasonic spray assistance, which is convenient for high-throughput microbial inoculation. Single colony culture. Background technique

[0002] Microorganisms are closely related to human life and are widely used in pharmaceutical and chemical industries, agricultural products, chemical energy, environmental protection and other fields. Traditional microbial pure culture methods include streak inoculation, coating inoculation, and liquid inoculation. However, these traditional inoculation methods have problems such as large workload, need for operating experience, and low throughput of single colony culture with good countability. For example, in the most commonly used coating and inoculation method...

Examples

Embodiment 1

[0050] The spray-assisted high-throughput microbial inoculation device in the present invention can be used with a solid medium when in use, that is, it consists of four parts: a spray generator, a sedimentation channel, a mask assembly and a solid medium (of course, the mask assembly is also optional or optional).

[0051] like figure 1 As shown, the spray device is attached to the threaded settling channel. In this embodiment, the ultrasonic atomizing sheet is preferably selected, and its outer diameter is 18 mm. By fixing and sealing the screw mouth of the fixed seat and the sedimentation channel, the leakage of spray droplets is prevented.

[0052] In this embodiment, the settling channel is preferably refitted with a 500ml plastic bottle, which is low in cost and easy to process. Cut off the bottom of the plastic bottle, sand it to a level, and directly and seamlessly connect with the solid medium to prevent the leakage of spray aerosol droplets. The spray distance pr...