Blood sample analyzer, blood sample analysis method and computer storage medium

Abstract

The present application discloses a blood sample analyzer, comprising: a sample transport device for transporting a sample rack containing a sample container; a first mixing device having a sample stirring component for stirring the blood sample in the sample container, the first mixing device being capable of driving the sample stirring component to mix the blood sample in the sample container; a second mixing device being capable of obtaining the sample rack or the second sample container on the sample rack, and being capable of driving the second sample container containing a constant amount of blood sample on the second mixing position to mix the blood sample; a control device being communicatively connected with the sample transport device, the first mixing device and the second mixing device, and controlling the actions of the sample transport device, the first mixing device and the second mixing device. In addition, the present application also discloses a blood sample mixing device, a blood sample analysis method, and a computer storage medium.

Description

Technical Field

[0001] The present application relates to the field of sample analysis, and in particular to a blood sample analyzer and a blood sample mixing method for mixing and analyzing extracted trace samples. Background Art

[0002] In the process of clinical diagnosis, it is often necessary to use an analytical device to measure blood, urine, and body fluid (ascites, cerebrospinal fluid, pleural effusion, etc.) samples collected from patients. Usually, the analytical device will specify the required sample volume in advance. Taking blood samples as an example, there are currently two ways to collect blood: venous blood collection and peripheral blood collection. The venous blood collection method collects a large amount of blood (≥1mL) and is usually suitable for adult patients; however, it is sometimes difficult to collect blood through a venous method for infants, children or critically ill patients. In this case, peripheral blood is often collected. The situation faced by peripheral blood collection is that the amount of blood that can be collected is small (in most cases ≤100μL).

[0003] When collecting blood, blood collection tubes containing anticoagulants are usually used to prevent blood coagulation. Blood is composed of blood cells and plasma. Due to the different specific gravities of blood cells and blood samples, anticoagulated blood will be stratified after being left to stand for a period of time. Therefore, the blood sample must be fully mixed before measurement, otherwise the measurement result will have a large deviation.

[0004] CN1334453A discloses a device for processing blood product samples, which has a shaking device for stirring the blood sample in a test tube. The shaking device clamps the test tube with a clamping assembly, and rotates the clamping assembly to make the test tube rotate continuously by 360 degrees, thereby continuously inverting the test tube upside down, thereby inverting and stirring the blood sample in the test tube, thereby stirring the blood sample in the test tube.

[0005] For normal blood samples (venous blood samples), due to the large amount of blood collected and good blood fluidity, when the blood collection tube is inverted, the venous blood will inevitably flow along the tube wall under the action of gravity. The multiple inversion method disclosed in CN1334453A can be used to make the blood flow back and forth along the tube wall to achieve mixing.

[0006] In CN1334453A, the same stirring operation is used for normal blood samples and trace blood samples. However, the inversion mixing method disclosed in CN1334453A will cause part of the blood to remain on the blood collection tube cap and the tube wall, resulting in blood sample loss. For normal samples such as venous blood samples, the proportion of lost blood samples to the total blood collection volume is very small, and it does not affect the measurement. However, for trace samples such as peripheral blood samples, because the blood collection volume of peripheral blood samples is small and the fluidity is poor, when the blood collection tube is inverted, the peripheral blood often adheres to the blood collection tube cap, the bottom of the blood collection tube or the tube wall and does not flow. The inversion mixing technology disclosed in the above-mentioned prior art will cause blood sample loss, which has an adverse effect on the measurement, and it is still difficult to effectively solve the problem of peripheral blood mixing. Therefore, even if the inversion mixing method disclosed in CN1334453A can fully mix the normal blood sample, it may be poorly mixed for the trace blood sample.

[0007] In order to solve the technical problem of poor stirring uniformity in CN1334453A, CN103675309A discloses a sample processing device, which has a stirring motor component and a hand component, and the stirring motor component drives the hand component to rotate, so that the sample container rotates between an inverted state and an upright state. In CN103675309A, in order to distinguish the different stirring uniformity requirements between a normal blood sample and a trace blood sample, the stirring time of the sample in the trace blood sample mode is made longer than the stirring time of the sample in the normal blood sample mode, so that the trace blood sample can be fully stirred.

[0008] Although in CN103675309A, the stirring time of the sample in the micro blood sample mode is longer than that in the constant blood sample mode so that the micro blood sample can be fully stirred, there is still a situation that the micro blood sample often adheres to the cap or the upper part of the tube wall of the blood collection tube.

[0009] Therefore, there are two problems in mixing peripheral blood using the inversion mixing technology disclosed in the above-mentioned prior art: (1) At present, most of the peripheral blood collection tubes in China use plastic caps, which do not support puncture (direct puncture of the plastic cap will damage the puncture needle, and plastic debris will fall into the blood collection tube and contaminate the blood sample). Therefore, the blood collection tube cap needs to be opened before measurement, and inverting the blood collection tube will cause the blood sample to flow out; (2) Even if some foreign manufacturers provide blood collection tubes with rubber caps that can support puncture, the cost of imported blood collection tubes is relatively high and cannot be widely used. The more serious problem is that inverting the blood collection tube will cause part of the blood sample to remain on the rubber cap, resulting in blood sample loss. Since the amount of peripheral blood collected is small, the lost blood sample accounts for a large proportion of the total blood collection volume, which can easily cause the analyzer to aspirate insufficient samples and affect the measurement results.

[0010] CN107121559A discloses a method for mixing a mixture of a peripheral blood sample and a diluent, wherein a sampling needle is inserted into a centrifuge tube for the mixture, and the mixture is mixed by automatic suction and discharge of the sampling needle.

[0011] For whole blood samples, it is not a homogeneous liquid, but is composed of plasma (generally accounting for about 55% of the volume) and blood cells (generally accounting for about 45% of the volume). Blood cells can be understood as tiny particles, and their density is generally slightly greater than that of plasma. Therefore, if the whole blood sample is added with an anticoagulant (to prevent blood from clotting) and left to stand for a period of time, the blood sample will be layered in the blood collection tube 92: plasma is in the upper layer, and blood cells are in the lower layer (refer to Figure 1 ). If the automatic suction and discharge method disclosed in CN107121559A is used to mix the whole blood sample, the sampling needle is in the plasma layer or the blood cell layer due to the stratification of the blood sample. When the sampling needle performs suction and discharge operations, it is often performed in one of the plasma layer or the blood cell layer, making it difficult to fully mix the plasma layer and the blood cell layer. In addition, the amount of suction and discharge each time by the sampling needle is small, and a long time is required for the mixing operation.

[0012] Based on the technical problems existing in the sample mixing technology disclosed in the above-mentioned prior art and the urgent market demand for fully automated measurement of peripheral blood, the present application proposes a sample analyzer and a sample mixing method, which can effectively achieve uniform mixing of trace whole blood samples such as peripheral blood samples. Summary of the invention

[0013] According to a first aspect of the present application, a blood sample analyzer is provided, comprising: a sample transport device for transporting a sample rack containing a first and / or second sample container; a first mixing device having a sample stirring component for stirring the blood sample in the first sample container, the first mixing device being capable of driving the sample stirring component to mix the blood sample in the first sample container containing a trace amount of blood sample on the sample rack at a first mixing position; a second mixing device being capable of acquiring the sample rack or the second sample container on the sample rack, and being capable of driving the second sample container containing a constant amount of blood sample on the second mixing position to mix the blood sample; and a control device being communicatively connected to the sample transport device, the first mixing device and the second mixing device, and controlling the actions of the sample transport device, the first mixing device and the second mixing device.

[0014] In a second aspect of the present application, a blood sample analyzer is provided, comprising: a sample chamber assembly having a sample chamber cover and a sample container fixing hole, for single sample injection of a trace blood sample or a macro blood sample placed on the sample container fixing hole; a mixing device having a sample stirring component for stirring the blood sample in the sample container, wherein the mixing device can drive the sample stirring component to mix the blood sample in the sample container.

[0015] According to a second aspect of the present application, a blood sample analyzer is provided, comprising: a first mixing device, capable of mixing a blood sample in a first sample container; a second mixing device, capable of mixing a blood sample in the second sample container in a manner different from that of the first mixing device; and a control device, which is communicatively connected to the first mixing device and the second mixing device and capable of performing the following operations: (1) determining whether it is a first measurement mode or a second measurement mode; (2) when it is determined that it is the first measurement mode, controlling the first mixing device to mix the blood sample in the first sample container; and (3) when it is determined that it is the second measurement mode, controlling the second mixing device to mix the blood sample in the second sample container.

[0016] The third aspect of the present application provides a blood sample analyzer, comprising: a sample transport device for transporting a sample rack containing a sample container; a mixing device having a sample stirring component for stirring the blood sample in the sample container, the mixing device being capable of driving the sample stirring component to mix the blood sample in the sample container; and a control device, communicatively connected to the sample transport device and the mixing device, to control the actions of the sample transport device and the mixing device.

[0017] The fourth aspect of the present application provides a blood sample analysis method for routine blood tests, comprising: transporting a sample container containing a blood sample to a mixing position; driving a sample stirring component of a first mixing device to mix the blood sample in the sample container; aspirating a predetermined sampling amount of the blood sample from the sample container on the mixing position to prepare a test sample for a routine blood test item; testing the test sample to obtain relevant indicators of the routine blood test item.

[0018] The fifth aspect of the present application provides a blood sample analysis method, including: a measurement mode determination step: determining whether the current measurement mode is the first measurement mode or the second measurement mode; a first detection sample preparation step: when it is determined to be the first measurement mode, controlling the first mixing device to drive the sample stirring component to mix the blood sample in the sample container, and aspirating the first sampling amount of the blood sample to prepare the first detection sample; a second detection sample preparation step: when it is determined to be the second measurement mode, controlling the second mixing device to mix the blood sample in the sample container, and aspirating the second sampling amount of the blood sample to prepare the second detection sample; and a detection step: detecting the first detection sample or the second detection sample.

[0019] In the sixth aspect of the present application, a blood sample analyzer is provided, comprising: a sample transport device for transporting a sample rack containing a sample container; a mixing device having a sample suction device for sucking and discharging a blood sample in a sample container, wherein the sample suction device can drive the sample suction target of the sample suction device to suck and discharging a blood sample in the sample container containing a trace blood sample at a sampling position to mix the blood sample; and a control device, which is communicatively connected with the sample transport device and the mixing device to control the actions of the sample transport device and the mixing device.

[0020] The seventh aspect of the present application provides a blood sample mixing method, comprising: a sample suction needle sucks an appropriate amount of air to form an isolation air column inside the sample suction needle; driving the sample suction needle downward to approach the bottom of a sample container; after driving the sample suction needle to suck an appropriate amount of blood sample, the sucked blood sample is returned to the sample container, so that the blood sample in the sample container forms a certain flow until the blood sample is mixed.

[0021] In an eighth aspect of the present application, a control device for a blood sample analyzer is provided, comprising: at least one processor; and a memory storing instructions executable by the at least one processor, wherein the instructions, when executed by the at least one processor, enable the blood sample analyzer to execute any of the methods described above.

[0022] In an eighth aspect of the present application, a computer storage medium is provided, storing computer executable instructions, wherein when the computer executable instructions are executed by at least one processor of a blood sample analyzer, the blood sample analyzer executes any of the methods described above.

[0023] Furthermore, the sample transport device can transport the sample rack containing the first and / or second sample containers to the first mixing position; the second mixing device can obtain the sample rack or the second sample container on the sample rack from the first mixing position and transport it to the second mixing position.

[0024] Furthermore, the first mixing position and the second mixing position are at the same position.

[0025] Furthermore, the head of the sample stirring component is cylindrical, paddle-shaped or polygonal.

[0026] Furthermore, the control device controls the sample stirring component to stir in one or a combination of the following modes: rotation, circular orbit, linear swing or up and down oscillation.

[0027] Furthermore, the sample stirring component can move up and down, and can move downward to the first sample container on the first mixing position to stir and mix.

[0028] Furthermore, the blood sample analyzer also includes: a cleaning component, used to clean the sample stirring component; preferably, the cleaning component includes a cleaning liquid inlet and a cleaning liquid outlet, so as to clean the sample stirring component located in the cleaning component; more preferably, the cleaning liquid outlet can also be used for vacuuming, so as to dry the sample stirring component.

[0029] Furthermore, the cleaning component includes a cleaning pool capable of cleaning the sample stirring component.

[0030] Furthermore, the blood sample analyzer also includes: a sample chamber assembly, including a sample chamber cover and a sample container fixing hole, which is used for single sample injection of a trace blood sample or a constant blood sample placed on the sample container fixing hole.

[0031] Furthermore, the blood sample analyzer also includes: the control device is also used to determine whether the current injection mode is the first injection mode or the second injection mode; when it is determined to be the first injection mode, the sample transport device is controlled to transport the sample rack containing the first and / or second sample containers; when it is determined to be the second injection mode, the sample compartment assembly is controlled to transport a single first and / or second sample container.

[0032] Furthermore, the sample transport device transports the sample rack containing the first sample container to a predetermined position, and the gripper of the second mixing device can grab the first sample container from the sample rack at the predetermined position to the first mixing position.

[0033] Furthermore, the blood sample analyzer also includes: a measurement mode setting device, used to set a first measurement mode and a second measurement mode; wherein the control device performs the following actions according to the setting of the measurement mode setting device: (1) determining whether it is the first measurement mode or the second measurement mode; (2) when it is determined to be the first measurement mode, controlling the first mixing device to mix the blood sample in the first sample container; (3) when it is determined to be the second measurement mode, controlling the second mixing device to grab the second sample container for mixing.

[0034] Furthermore, the blood sample analyzer also includes: a sample suction device, used to aspirate the mixed blood sample from the sample container; when it is judged to be the first measurement mode, the control device controls the sample suction device to aspirate the blood sample of the first blood collection volume from the first sample container; when it is judged to be the second measurement mode, the control device controls the sample suction device to aspirate the blood sample of the second blood collection volume from the second sample container; wherein, the first sampling volume is less than the second sampling volume; preferably, the first sampling volume is 5-50μL, and more preferably, it is 15-35μL.

[0035] Furthermore, the measurement mode setting device is also used to set a third measurement mode; when the control device determines that it is the third measurement mode, the first mixing device is controlled to mix the pre-diluted blood sample in the first sample container.

[0036] Furthermore, the first mixing device mixes the trace whole blood sample in the first sample container; preferably, the trace whole blood sample in the first sample container is 30-250 μL, more preferably 50-200 μL, and even more preferably 50-100 μL.

[0037] Furthermore, after the first mixing device mixes the trace blood sample in the first sample container, the fluctuation range of the hemoglobin value of the trace blood sample after repeated measurements does not exceed ±2g / L.

[0038] Furthermore, the blood sample analyzer is only used to process trace whole blood samples and pre-diluted blood samples.

[0039] Furthermore, the blood sample analyzer also includes: a sample suction device, used to aspirate the mixed blood sample from the sample container; a sample preparation device, used to prepare the blood sample aspirated by the sample suction device into a test sample; and a control device, which is communicatively connected with the mixing device, the sample suction device and / or the sample preparation device to control the operation of the mixing device, the sample suction device and / or the sample preparation device.

[0040] Furthermore, the blood sample analyzer also includes: a measurement mode setting device, which is used to set a first measurement mode and a second measurement mode; wherein the control device performs the following actions according to the setting of the measurement mode setting device: (1) determining whether it is the first measurement mode or the second measurement mode; (2) when it is determined that it is the first measurement mode, controlling the sample suction device to aspirate a first sampling amount of the blood sample from the sample container on the sample rack, and controlling the sample preparation device to prepare a first test sample; (3) when it is determined that it is the second measurement mode, controlling the sample suction device to aspirate a second sampling amount of the blood sample from the sample container on the sample rack, and controlling the sample preparation device to prepare a second test sample; wherein the first sampling amount is less than the second sampling amount; preferably, the first sampling amount is 5-50 μL, and more preferably, it is 15-35 μL.

[0041] Furthermore, the measurement mode setting device is also used to set a third measurement mode; when the control device determines that it is the third measurement mode, it controls the blood analyzer to pre-dilute the blood sample, then controls the sample aspirating device to aspirate a third sampling amount of the pre-diluted blood sample from the sample container on the sample rack, and controls the sample preparation device to prepare a third test sample.

[0042] Furthermore, the control device can also perform the following tasks: (1) determine whether it is the third measurement mode; (2) when the control device determines that it is the third measurement mode, control the first mixing device to mix the pre-diluted blood sample in the sample container.

[0043] Furthermore, the blood sample is a whole blood sample.

[0044] Furthermore, the blood sample analyzer also includes: a sample chamber assembly, having a sample chamber cover and a sample container fixing hole, for single sample injection of a blood sample placed on the sample container fixing hole; and / or a sample transport device, for transporting a sample rack containing the first sample container and / or the second sample container.

[0045] Furthermore, the control device can also perform the following operations: (1) determine whether it is the first injection mode or the second injection mode; (2) when it is determined that it is the first injection mode, control the sample transport device to transport the sample rack containing the sample container; (3) when it is determined that it is the second injection mode, control the sample compartment assembly to transport a single sample container to the blood analyzer.

[0046] Furthermore, the blood sample analysis method also includes: determining whether the current measurement mode is the first measurement mode or the second measurement mode; when it is determined to be the first measurement mode, aspirating a first sampling amount of the mixed blood sample from the first sample container, and preparing a first detection sample; when it is determined to be the second measurement mode, aspirating a second sampling amount of the mixed blood sample from the second sample container, and preparing a second detection sample; wherein, the first sampling amount is less than the second sampling amount; preferably, the first sampling amount is 5-50 μL, and more preferably 15-35 μL.

[0047] Furthermore, the blood sample analysis method further includes: determining whether the current measurement mode is a third measurement mode; when it is determined to be the third measurement mode, aspirating a third sampling amount of the mixed pre-diluted blood sample from the first sample container, and preparing a third test sample.

[0048] Furthermore, the blood sample analysis method also includes: determining whether the current injection mode is the first injection mode or the second injection mode; when it is determined to be the first injection mode, the sample container is transported by the sample transport device; when it is determined to be the second injection mode, the single sample container is transported to the blood analyzer by the sample compartment assembly.

[0049] Furthermore, the blood sample analysis method further comprises: a second mixing device obtains a second sample container and performs inversion mixing.

[0050] Furthermore, in the measurement mode determination step, it is also determined whether the current measurement mode is the third measurement mode; a third detection sample preparation step: when it is determined to be the third measurement mode, the first mixing device is controlled to mix the pre-diluted blood sample in the sample container, and aspirate a third sampling amount of the pre-diluted blood sample to prepare a third detection sample; in the detection step, the third detection sample is detected.

[0051] Furthermore, the blood sample analysis method also includes: an injection mode determination step: determining whether the current injection mode is the first injection mode or the second injection mode; a sample rack transport step: when it is determined to be the first injection mode, controlling the sample transport device to transport the sample rack containing the sample container to a predetermined position, and transporting the mixed sample container to the first sampling position; a sample chamber component closing step: when it is determined to be the second injection mode, closing the sample chamber component, and the sample container is transported to the second sampling position.

[0052] Furthermore, in the first detection sample preparation step, the second mixing device moves the sample container on the sample rack transported to the predetermined position by the sample transport device to the first mixing position for mixing; in the second detection sample preparation step, the second mixing device grabs the sample rack transported to the predetermined position by the sample transport device or the sample container on the sample rack for inversion mixing.

[0053] Furthermore, the sample aspirating device also includes a suction and exhalation driving device for driving the sample aspirating needle to aspirate and exhale the blood sample in the sample container for mixing.

[0054] Furthermore, the suction and exhalation driving device is a syringe.

[0055] Furthermore, the blood analyzer includes a mixing device that can perform inversion mixing on a constant blood sample.

[0056] Furthermore, the suction and exhalation driving device can drive the sample aspirating needle to absorb a proper amount of air before mixing the blood sample in the sample container, so that an isolation air column is formed inside the sample aspirating needle.

[0057] Furthermore, the sample aspirating device also includes a sample aspirating needle air-drying device for air-drying the outer wall of the sample aspirating needle.

[0058] Furthermore, the sample aspirating device also includes a sample aspirating needle position sensor for sensing the downward position of the sample aspirating needle.

[0059] Furthermore, the blood sample mixing method also includes: determining whether the injection mode is the first injection mode or the second injection mode; if it is the first injection mode, the sample transport device transports the sample container on the sample rack to the first sampling position; if it is the second injection mode, the sample bin assembly transports the single sample container to the second sampling position.

[0060] Furthermore, the blood sample mixing method further comprises: air-drying the outer wall of the sample aspirating needle before the sample aspirating needle aspirates an appropriate amount of air.

[0061] The device and method disclosed based on the above technical solution can effectively achieve uniform stirring of trace samples such as peripheral blood samples, and can also solve the technical problem that the blood sample remains on the rubber cap, causing the loss of trace blood samples such as peripheral blood, resulting in insufficient sample aspiration by the analyzer and affecting the measurement results. It can also enable the analysis instrument to be miniaturized while solving the existing technical problems. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] Figure 1 A schematic diagram of the stratification phenomenon of blood samples in blood collection tubes;

[0063] Figure 2This is an oblique view of the appearance of a blood sample analyzer according to one embodiment of the present application;

[0064] Figure 3 and Figure 4 This is a schematic structural diagram of a sample transport device according to one embodiment of the present application;

[0065] Figure 5 This is a schematic structural diagram of a first mixing device according to an embodiment of the present application;

[0066] Figure 6 This is a schematic structural diagram of a second mixing device according to an embodiment of the present application;

[0067] Figure 7 This is a schematic structural diagram of a sample suction device according to one embodiment of the present application;

[0068] Figures 8 to 11 This is a schematic structural diagram of a container rotating code scanning device according to an embodiment of the present application;

[0069] Figures 12-13 This is a schematic diagram of the working principle of a container rotating code scanning device according to an embodiment of the present application;

[0070] Fig.14 This is a schematic diagram of the sample rack structure of this application;

[0071] Fig.15 A schematic diagram of a sample rack with sample containers installed in the present application;

[0072] Fig.16 A schematic diagram of a sample rack equipped with a micro-blood collection tube according to the present application;

[0073] Fig.17 A schematic diagram of an adapter structure of the present application;

[0074] Fig.18 This is another schematic diagram of the adapter structure of the present application;

[0075] Fig.19 This is a structural block diagram of the control device of this application;

[0076] Fig. 20 and Fig.21 This is a main flow chart of an example of blood sample analysis and processing by the blood sample analyzer of this application;

[0077] Fig. 22 This is a schematic diagram of the setting interface of the blood analyzer of this application;

[0078] Fig.23 This is an example diagram of the mixing operation of the first mixing device in one embodiment of the present application;

[0079] Fig.24This is a schematic flow chart of the mixing process in step S11 in one embodiment of the present application;

[0080] Fig.25 This is an example diagram of cleaning the stirring component of the first mixing device in one embodiment of the present application;

[0081] Fig.26 This is an example diagram of the sample aspirating process in step S13 in the first injection mode in one embodiment of the present application;

[0082] Fig. 27 This is an example diagram of the sample aspirating process in step S22 in the second injection mode in one embodiment of the present application.

[0083] Fig.28 and Fig.29 This is a main flow chart of another example of analyzing and processing blood samples by the blood sample analyzer of the present application;

[0084] Fig.30 This is a main flow chart of another example of analyzing and processing blood samples by the blood sample analyzer of the present application;

[0085] Fig.31 This is a graphical representation of the analysis data of a 100 μL micro-volume whole blood sample;

[0086] Fig.32 6 micro whole blood samples of different volumes are mixed by the first mixing device 11 of the first embodiment of the present application, and the data of HGB is detected by the first measurement mode;

[0087] Fig.33 This is a schematic diagram of the structure of a mixing device in one embodiment of the present application;

[0088] Fig.34 This is a schematic structural diagram of another sample suction device according to one embodiment of the present application. DETAILED DESCRIPTION

[0089] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be interpreted as limiting the present application.

[0090] Those skilled in the art will appreciate that, unless expressly stated, the singular forms "a", "an", "said" and "the" used herein may also include plural forms. It should be further understood that the term "comprising" used in the specification of the present application refers to the presence of the features, integers, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or groups thereof. It should be understood that when we refer to an element as being "connected" to another element, it may be directly connected to the other element, or there may be intermediate elements. In addition, the "connection" used here may include wireless connections. The term "and / or" used here includes all or any unit and all combinations of one or more associated listed items.

[0091] It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as those generally understood by those skilled in the art to which this application belongs. It should also be understood that terms such as those defined in general dictionaries should be understood to have meanings consistent with those in the context of the prior art, and will not be interpreted with idealized or overly formal meanings unless specifically defined as here.

[0092] Considering that peripheral blood samples have a small amount of blood and poor fluidity, when the blood collection tube is inverted, the peripheral blood often adheres to the bottom or wall of the blood collection tube and does not flow, and inversion mixing easily causes blood sample spillage or loss. The traditional inversion mixing technology is difficult to solve the problem of peripheral blood mixing. Based on this, the present application proposes a micro-sample automatic mixing method, a mixing device and an analyzer with a micro-sample automatic mixing function, which achieves the mixing of micro-samples by driving a stirring component to move in a sample container.

[0093] (Implementation Method 1)

[0094] Figure 2 FIG. 2 is an oblique view of the appearance of the blood sample analyzer of this embodiment. Figure 2 As shown, the blood sample analyzer 1 includes an instrument body, a housing 30, a sample transport device 17 disposed in front of the instrument body, etc. Among them, the housing 30 is provided with a display component 31, an operation button 32, and an operation button 33, etc. The display component 31 can be a touch screen that can be operated by touch, and the input device 23 (such as a soft keyboard, etc.) can be displayed by touching the display component 31 (refer to Fig.19 ). In this embodiment, the input device 23 may also be independently provided as hardware.

[0095] The above-mentioned instrument body can be basically accommodated in the casing 30, including a first mixing device 11 for mixing the blood sample in the sample container (blood collection tube) 91 and a second mixing device 12 for mixing the blood sample in the sample container (blood collection tube) 92, a sample suction device 13 for sucking the blood sample mixed by the first mixing device 11 or the second mixing device 12 from the sample container 91 (92), a container rotating scanning device (including a container clamping component 14, a container rotating component 15, and a scanner 16) for obtaining the sample rack 80 and the sample coding information on the label of the sample container 91 (92) transported to the scanning position (not shown) by the sample transport device 17, a sample preparation device (not shown) for preparing a test sample from the blood sample sucked by the sample suction device 13, a detector (not shown) for detecting blood cells in the blood from the test sample prepared by the sample preparation device, and a control device 21 that can be electrically connected to the display component 31, the operation button 32, the operation button 33 and the corresponding components of the instrument body, etc.

[0096] In this embodiment, the blood sample analyzer 1 may also include a sample chamber assembly 18, which is used for single sample injection of a trace whole blood sample or a normal blood sample, and is usually used for measuring emergency queue jumping samples. The sample chamber assembly 18 has a sample chamber cover 181 and a sample container fixing hole 182. When it is necessary to measure the emergency queue jumping sample, the sample chamber cover 181 can be opened to place the sample container containing the emergency queue jumping sample into the sample container fixing hole 182 to fix the sample container, or to remove the sample container from the sample container fixing hole 182. The aperture of the sample container fixing hole 182 is slightly larger than the sample container or adapter 81 (82) to be placed (see Fig.17 and Fig.18 )’s outer diameter.

[0097] Figure 3 and Figure 4 FIG. 1 is a schematic diagram of the structure of the sample transport device of this embodiment. Figure 3 As shown, the sample transport device 17 includes: a sample rack supporting component 171 , a sample rack feeding device 172 , a sample rack bidirectional sample transport device 173 , and a sample rack feeding device 174 .

[0098] The sample rack supporting part 171 includes: a pre-analysis sample rack storage area 1711 for storing a plurality of sample racks 80 fixed with sample containers containing pre-analysis samples, a post-analysis sample rack storage area 1712 for storing a plurality of sample racks 80 fixed with sample containers containing post-analysis samples, and a sample analysis area (not shown) located between the pre-analysis sample rack storage area 1711 and the post-analysis sample rack storage area 1712. A sample rack delivery turning area 1711a is provided on one side of the pre-analysis sample rack storage area 1711, and a sample rack delivery turning area 1712a is provided on the post-analysis sample rack storage area 1712.

[0099] The sample rack feeding device 172 includes sample rack feeding components 1721 and 1722. The sample rack feeding components 1721 and 1722 can move in the Y2 direction to push the sample racks 80 stored in the pre-analysis sample rack storage area 1711 to the sample rack feeding turning area 1711a one by one. The sample rack feeding components 1721 and 1722 are driven by a stepper motor (not shown). The sample rack 80 entering the sample rack feeding turning area 1711a will be transported by the sample rack bidirectional sample transport device 173 along the X1 direction. The sample container 91 (92) containing the sample entering the analysis area will be transported to the scanning position in turn and scanned by the container rotating scanning device 14~16, and then sent to the predetermined position to mix the sample container 91 by the first mixing device 11 or the sample container 92 will be grabbed to a certain position by the second mixing device 12 for mixing, and then sent to the sampling position to have the sample suction device 13 suck the blood sample mixed by the first mixing device 11 or the second mixing device 12 from the sample container 91 (92), the blood sample sucked by the sample suction device 13 is prepared into a test sample by the sample preparation device, and the blood cells in the test sample prepared by the sample preparation device are detected by the detector.

[0100] After the sample rack 80 with the sample container containing the sample fixed therein is transported to the sample rack delivery turning area 1712a by the sample rack bidirectional sample transport device 173, the sample rack delivery component 1741 of the sample rack delivery device 174 moves horizontally along the Y1 direction to push the sample rack 80 to the post-analysis sample rack storage area 1712. The sample rack delivery component 1741 is driven by a stepper motor (not shown).

[0101] Figure 5 Schematic diagram of the structure of the first mixing device of this embodiment. The first mixing device 11 can be movably mounted on the housing 30 or other bracket (not shown) of the blood sample analyzer 1, and can be moved up and down, left and right or rotated by a motor drive. Figure 5 As shown, the first mixing device 11 includes: a stirring component driving motor 111, a sample stirring component 112, and a cleaning component 113. The stirring component motor 111 can be a stepper motor, a servo motor or a DC motor. In this embodiment, preferably, the motor 111 is a stepper motor.

[0102] The sample stirring member 112 may be a stirring rod with a cylindrical, paddle-shaped, polygonal or other head shape, and is driven by the stirring member motor 111 to perform stirring in one or a combination of several modes such as rotation, circular orbit, linear swing or up and down oscillation. At the same time, the sample stirring member 112 may drive the mixing device 11 to move up and down and left and right under the drive of the mixing device driving motor (not shown).

[0103] A cleaning component 113 for cleaning the sample stirring component 112. In the present embodiment, preferably, the cleaning component 113 includes a cleaning liquid inlet 1131 and a cleaning liquid outlet 1132. When the cleaning component 113 completes the stirring operation of the blood sample in a blood sample container 91, the cleaning component 113 cleans the sample stirring component 112 therein to prevent contamination when the blood sample in the next blood sample container is stirred. Specifically, when the cleaning component 113 completes the stirring operation of the blood sample in a blood sample container 91, the cleaning liquid is input through the cleaning liquid inlet 1131 to clean the sample stirring component 112, and the cleaned cleaning liquid is discharged through the cleaning liquid outlet 1132 to recover the cleaning liquid. At the same time, in the present embodiment, after the sample stirring component 112 is cleaned with the cleaning liquid, the sample stirring component 112 can also be dried, for example, by exhausting air through the cleaning liquid outlet 1132 to air dry the sample stirring component 112. Directly utilizing the cleaning liquid discharge port 1132 for vacuum drying can save air drying equipment and make the device more compact.

[0104] In this embodiment, the sample in the sample container is mixed by driving the sample stirring component 112 of the first mixing device 11, so that the components of each layer in the whole blood sample in the sample container 91 can be quickly mixed. Moreover, during the mixing process, the bottom of the sample container 91 does not exceed the tube mouth, and the sampling needle of the analyzer 1 does not contact the blood sample in the sample container 91, and the mixing device 11 can achieve the mixing of trace samples without causing the blood sample to be splashed or the blood sample to be stained with the tube cap.

[0105] In this embodiment, before using the first mixing device 11 to stir the blood sample in the sample container 91 , it is necessary to open the sealed cover of the sample container 91 , or use a sample container 91 without a sealed cover.

[0106] Preferably, the sample container 91 contains a trace amount of blood sample, usually greater than or equal to 50 μL and less than or equal to 250 μL, for example, 100 μL; the sample contained in the sample container can be a whole blood sample or a pre-diluted sample; the sample contained in the sample container 91 can be a trace amount of peripheral blood or a trace amount of venous blood, as long as the volume is less than or equal to 250 μl, it is suitable for mixing with the first mixing device 11, and is particularly suitable for mixing with a trace amount of whole blood sample with a volume greater than or equal to 50 μL and less than or equal to 200 μL.

[0107] Figure 6Schematic diagram of the structure of the second mixing device of this embodiment. The second mixing device 12 can grab the constant blood sample container placed on the sample rack 80 and transported to the predetermined position of the analyzer 1 by the sample transport device 17, and mix the sample therein by inverting the mixing method. The constant blood sample can be a venous blood collection tube 92, or another type of venous blood collection tube; the constant blood sample container contains a sample of a second sample volume (constant blood sample) that is significantly larger than the first sample volume (micro blood sample), and the second sample volume is usually ≥1mL; the sample contained in the constant blood sample container is a venous whole blood sample.

[0108] like Figure 6 As shown, the second mixing device 12 includes: a clamp 1201, a first support frame 1211, a second support frame 1212, a third support frame 1213, a stepper motor 1221~1223, a linear slide rail 1231~1232, an annular synchronous toothed belt 1241~1243 wound on a synchronous wheel, a position sensor 1121~1125, a sensor sensing sheet 1261~1263, and a rotating shaft 1271.

[0109] The first support frame 1211 is the main support member of the second mixing device 12, and is used to fix the stepper motor 1221, the linear slide 1231 and the position sensors 1251-1252, and the first support frame 1211 is fixed to the front plate of the analyzer 1 by screws; the linear slide 1231 is placed along the Z1 and Z2 directions, and the second support frame 1212 and the sensor sensing sheet 1261 are connected to the slider of the linear slide 1231, and can slide along the Z1 or Z2 direction; the second support frame is used to fix the stepper motor 1222, the linear slide 1232, and the position sensors 1253-125 4; the linear guide rail 1232 is placed along the Y1 and Y2 directions, the third support frame 1213 and the sensor sensing sheet 1262 are connected to the slider of the linear guide rail 1232, and can slide along the Y1 or Y2 direction; the third support frame 1213 is used to fix the stepper motor 1223 and the position sensor 1255, and the rotating shaft 1271 is fixed to the third support frame 1213 in a rotating connection manner, and the rotating shaft 1271 can rotate along the R7 or R8 direction; the clamp 1201 and the sensor sensing sheet 1263 are fixedly connected to the rotating shaft 1271, and can rotate along the R7 or R8 direction following the rotating shaft 1271.

[0110] The annular synchronous toothed belt 1241 is driven by the rotation of the stepping motor 1221 and rotates under the guidance of two synchronous wheels. The second support frame 1212 is connected to the annular synchronous toothed belt 1241. Under the drive of the stepping motor 1221, the second support frame 1212 can drive the clamping jaw 1201 and the sensor sensing sheet 1261 to move along the Z1 or Z2 direction; the position sensors 1251 and 1252 cooperate with the sensor sensing sheet 1261 to realize the positioning of the clamping jaw 1201 along the Z1 or Z2 direction. When the second support frame 1212 drives the clamping jaw 1201 to move along the Z1 direction, the position sensor 1252 is used for positioning, and when the second support frame 1212 drives the clamping jaw 1201 to move along the Z2 direction, the position sensor 1251 is used for positioning.

[0111] The annular synchronous toothed belt 1242 is driven by the stepper motor 1222 and rotates under the guidance of two synchronous wheels. The third support frame 1213 is connected to the annular synchronous toothed belt 1242. Under the drive of the stepper motor 1222, the third support frame 1213 can drive the clamping jaw 1201 and the sensor sensing sheet 1262 to move along the Y1 or Y2 direction; the position sensors 1253 and 1254 cooperate with the sensor sensing sheet 1262 to realize the positioning of the clamping jaw 1201 along the Y1 or Y2 direction. When the third support frame 1213 drives the clamping jaw 1201 to move along the Y1 direction, the position sensor 1254 is used for positioning, and when the third support frame 1213 drives the clamping jaw 1201 to move along the Y2 direction, the position sensor 1253 is used for positioning.

[0112] The annular synchronous toothed belt 1243 is driven by the stepping motor 1223 and rotates under the guidance of two synchronous wheels. The rotating shaft 1271 drives the clamping jaw 1201 to rotate along the R7 or R8 direction under the drive of the stepping motor 1223; the position sensor 1255 cooperates with the sensor sensing sheet 1263 to realize the positioning of the clamping jaw when it moves along the R8 direction.

[0113] Figure 7 FIG. 2 is a schematic diagram of the structure of the sample suction device of this embodiment. Figure 7 As shown, the sample suction device 13 is used to suck blood samples from the sample container 91 (92) sent to the sampling position of the analyzer 1 by the sample transport device 17 for sample preparation.

[0114] like Figure 7 As shown, the sample suction device 13 includes: a sample suction needle 135, a sample suction needle moving assembly 131, a stepper motor 1301, synchronous wheels 1302 and 1303, an annular synchronous toothed belt 1304 wound around the synchronous wheels 1302 and 1303, a linear guide rod 1305 placed along the Y1 and Y2 directions, a position sensor 1306, etc.

[0115] The sample suction needle moving assembly 131 is connected to the annular synchronous toothed belt 1304 through a connecting piece. The annular synchronous toothed belt 1304 is driven by the rotation of the stepper motor 1301 and rotates under the guidance of the two synchronous wheels 1302 and 1303. The sample suction needle moving assembly 131 can drive the sample suction needle 135 to move along the Y1 or Y2 direction under the drive of the stepper motor 1301. The initial position of the sample suction needle moving assembly 131 in the Y1 and Y2 directions is positioned by the position sensor 1306 and the sensor sensing sheet 1318 fixed to the sample suction needle moving assembly 131.

[0116] The sample suction needle moving assembly 131 includes: a stepper motor 1311, a screw rod 1312, a nut 1323, a linear guide rail 1314, a sample suction needle fixing component 1315, a position sensor 1316, and a sensor sensing sheet 1317, etc.

[0117] The sample suction needle 135 is fixed on the sample suction needle fixing component 1315, and the sample suction needle fixing component 1315 is fixed on the linear slide rail 1314 placed along Z1 and Z2 by screws. At the same time, the nut 1313 is stuck in the slot set in the sample suction needle fixing component 1315, and there is no relative rotation between the nut 1313 and the sample suction needle fixing component 1315. The lead screw 1312 is connected to the rotating shaft of the stepper motor 1311 by screws. The stepper motor 1311 can drive the screw 1312 to rotate, and drive the sample suction needle fixing component 1315 to move with the sample suction needle 135 along the Z1 or Z2 direction. The initial position of the sample suction needle 135 in the Z1 and Z2 directions is positioned by the position sensor 1317 and the optical coupling sensor sensing sheet (not shown) set on the sample suction needle fixing component 1315.

[0118] Driven by the stepper motor 1301 and the stepper motor 1311, the sample aspirating needle 135 can move two-dimensionally along the Y1, Y2 directions and the Z1, Z2 directions, so as to realize the functions of aspirating blood samples from the sample container and distributing blood samples into the sample preparation device.

[0119] Figures 8 to 11 This is a schematic diagram of the structure of the container rotating code scanning device of this embodiment; Figures 12-13 Schematic diagram of the working principle of the container rotating code scanning device of this embodiment.

[0120] The container rotating scanning devices 14 to 16 (where 14 is a container clamping component, 15 is a container rotating component, and 16 is a scanner) are used to obtain the sample coding information on the sample container label sent to the scanning position of the analyzer 1 by the sample transport device 17, which is used for sample information management of the analyzer.

[0121] like Figure 8As shown, the container clamping assembly 14 includes: a stepper motor 141, a driven wheel bracket 142, a linear slide 143, and two driven wheels 144a and 144b. The driven wheels 144a and 144b are rotatably fixed to the driven wheel bracket 142, and the driven wheel bracket 142 is fixed to the slider of the linear slide 143. The linear slide 143 is arranged along the Y1 and Y2 directions. Under the drive of the stepper motor 141, the driven wheel bracket 142 can move the driven wheels 144a and 144b along the Y1 and Y2 directions. In addition, the driven wheel bracket 142 is provided with a notch 1421 for avoiding the scanning window of the barcode scanner 16.

[0122] like Fig. 9 As shown, the container rotating assembly 15 includes: a stepper motor 151, a rotating wheel 152, a rubber pad 153, and a coupling 154. The rotating wheel 152 is connected to the rotating shaft of the stepper motor 151 through the coupling 154. Under the drive of the stepper motor 151, the rotating wheel 152 can rotate counterclockwise or clockwise. The rubber pad 153 sleeved on the outer ring of the rotating wheel 152 is to increase the friction between the rotating wheel 152 and the container.

[0123] like Fig.10 As shown, in the initial position of the container pressing assembly 14, driven by the stepping motor 141, the driven wheel bracket 142 can move the driven wheels 144a and 144b along the Y1 direction to squeeze the sample container toward the rotating wheel 152 of the container rotating assembly 15 (refer to Fig.11 At this time, if the rotating wheel 152 of the rotating assembly 15 is driven by the stepping motor 151 to rotate around its center point O1 along the direction R11, under the action of friction, the sample container, the rollers 144a and 144b rotate around their respective center axes O2, O3 and O4 along the directions R12, R13 and R14 respectively; or if the stepping motor 151 drives the rotating wheel 152 to rotate around its center point O1 along the direction R11', the sample container, the rollers 144a and 144b rotate around their respective center axes O2, O3 and O4 along the directions R12', R13' and R14' respectively (refer to Fig.12 During the rotation of the sample container, the barcode label attached to the sample container will face the barcode reader 16 at one stage, and the barcode reader 16 can read the number information of the barcode label on the sample container (refer to Fig.13 ).

[0124] In this embodiment, the container rotating scanning device can support scanning of sample containers with suitable height and inner diameter, which can be placed in a test tube rack and have barcodes attached to the tube wall, preferably slender sample containers.

[0125] Fig.14 FIG. 1 is a schematic diagram of the sample rack structure of this embodiment. Fig.14As shown, the sample rack 80 is provided with fixing holes 801a for fixing sample containers, and each fixing hole 801a is provided with an opening 801b correspondingly, and the opening 801b serves as a scanning window for the barcode label of the sample container; in addition, the sample rack 80 is also specially provided with a sample rack label pasting area 802, and the sample rack label pasting area 802 can be pasted with a barcode label, a QR code label, or an RFID label. Preferably, the coding information of the label of the sample rack 80 includes measurement mode information.

[0126] The sample rack 80 can directly fix a plurality of venous blood collection tubes 92 or micro blood collection tubes 91 (see Fig.15 ), or the micro-blood collection tube 91 can be fixed by the adapter 81 (see Fig.16 ).

[0127] Adapter 81 (refer to Fig.17 ) is provided with a fixing hole 811 for fixing the micro-sampling tube 91, and a step 812 is provided to prevent the adapter 81 from falling during the ascending process of the clamping jaw 1201, and a cavity 814 is provided at the bottom to reduce the weight of the adapter. Compared with the adapter 82, the adapter 81 has an additional limiting portion 813 that can block the tube cap of the micro-sampling tube 91. When the micro-sampling tube 91 is placed in the fixing hole 811 of the adapter 81, the connecting portion 913 of the micro-sampling tube 91 needs to be inserted into the limiting portion 813 of the adapter 81. Since the tube cap and the tube body of the micro-sampling tube 91 are inseparable, in order to prevent the tube cap from covering the tube body under the restoring force of the connecting portion, the tube cap is restricted by the limiting portion 813 of the adapter 81 to prevent the sampling needle from being pierced by the tube cap when entering the micro-sampling tube 91 to absorb the sample. Preferably, in order to prevent the connecting portion of the micro-sampling tube 91 from escaping from the limiting portion 813 of the adapter 81 when the adapter 81 is stirred and mixed, the limiting portion 813 is set in a Z shape. The outer diameter of the adapter 81 is smaller than the diameter of the sample container fixing hole 801a of the sample rack 80, and the inner diameter of the fixing hole 811 of the adapter 81 is slightly larger than the outer diameter of the fixed micro-blood collection tube 91; the outer diameter of the adapter 81 can be equal to the outer diameter of the adapter 82.

[0128] Adapter 82 (refer to Fig.18 ) is provided with a fixing hole 821 for fixing the micro-sampling tube 91, and a step 822 (whose function is the same as the step 812 of the adapter 81); in addition, the adapter 82 is provided with a cavity 823 at its bottom in order to reduce weight. The outer wall diameter of the adapter 82 is smaller than the hole diameter of the fixing hole 801a of the sample holder 80, and the inner diameter of the fixing hole 821 of the adapter 82 is slightly larger than the outer diameter of the tube body of the fixing micro-sampling tube 91.

[0129] The above-mentioned micro-blood collection tube 91, whether collecting peripheral blood by capillary or by scraping blood, can collect a small amount of blood sample (in most cases ≤100 μL, and in a few cases 200-250 μL).

[0130] In this embodiment, the sample container 91 can be one or more of the micro-blood collection tubes, or can be another type of micro-tube; the sample volume of the micro-sample is usually ≤250μL, preferably 30-250μL, more preferably 50-200μL, and even more preferably 50-100μL; the micro-sample can be a whole blood sample or a pre-diluted sample; the micro-sample can be a trace amount of peripheral blood or a trace amount of venous blood.

[0131] Fig.19 21 is a structural block diagram of the control device 21. Fig.19 As shown, the control device 21 is mainly composed of a CPU 211a, a ROM 211b, a RAM 211c, a hard disk 211d, a reading device 211e, an input / output interface 211f, a communication interface 211g, and an image output interface 211h. The CPU 211a, the ROM 211b, the RAM 211c, the hard disk 211d, the reading device 211e, the input / output interface 211f, the communication interface 211g, and the image output interface 211h are connected via a bus 211i.

[0132] The CPU 211 a can execute computer programs stored in the ROM 211 b and computer programs downloaded to the RAM 211 c. The CPU 211 a executes application programs 214 a , 214 b , and 214 c , which will be described later, thereby fulfilling the function of the control device 21 .

[0133] ROM211b is composed of a mask ROM, PROM, EPROM, EEPROM, etc., and stores the computer program executed by CPU211a and the data required therefor.

[0134] The RAM 211 c is composed of an SRAM or a DRAM, etc. The RAM 211 c is used to read computer programs stored in the ROM 211 b and the hard disk 211 d. The RAM 211 c can also be used as a workspace when the CPU 211 a executes these computer programs.

[0135] The hard disk 211d is installed with various computer programs such as an operating system and application programs for the CPU 211a to execute, and data used when executing the computer programs. The first mixing processing program 214a for the first mixing device 11, the second mixing processing program 214b for the second mixing device 12, and the sample transport operation processing program 214c for the sample transport device 17 are also installed in the hard disk 211d. The CPU 211a executes these application programs 214a to 214c to control the operation of each part of the first mixing device 11, the second mixing device 12, and the sample transport device 17.

[0136] The reading device 211e is composed of a floppy disk drive, a CD-ROM drive, or a DVD-ROM drive, and can read computer programs or data stored in the portable storage medium 214. The portable storage medium 214 stores application programs 214a to 214c, and the control device 21 can read the application programs 214a to 214c from the portable storage medium 214 and install the application programs 214a to 214c into the hard disk 211d.

[0137] The above-mentioned application programs 214a~214c can be provided not only by the portable storage medium 214, but also from an external machine that is communicably connected to the control device 21 through an electronic communication line (whether wired or wireless) via the electronic communication line.

[0138] The hard disk 211d is installed with an operating system that can provide a graphical user interface. In the following description, the application programs 214a-214c are all run on the above operating system.

[0139] The input / output interface 211 f includes a serial interface, a parallel interface, and an analog interface including a D / A converter, an A / D converter, etc. The input / output interface 211 f is connected to the input device 23 , and the user can input data to the control device 21 using the input device 23 .

[0140] The communication interface 211g is a wired or wireless communication interface. The control device 21 can transmit data with the first mixing device 11, the second mixing device 12 and the sample transport device 17 using a certain communication protocol through the communication interface 211g.

[0141] The image output interface 211h is connected to the display unit 31 composed of LCD, CRT or the like, and outputs a video signal corresponding to the image data received from the CPU 211a to the display unit 31. The display unit 31 displays an image (interface) according to the input video signal.

[0142] The control device 21 controls the operations of the first mixing device 11 , the second mixing device 12 and the sample transport device 17 through the above structure.

[0143] Fig. 20 and Fig.21 The main flow chart of an example of blood sample analysis and processing by the blood sample analyzer 1 is shown in FIG. Fig. 20 and Fig.21 As shown, firstly, the power of the blood sample analyzer 1 is turned on, and the control device 21 starts initialization (step S1). In this initialization step, the program is initialized, the liquid circuit components of the blood sample analyzer 1 are initialized, the pipeline is cleaned, and the driving part is reset.

[0144] Next, in step S2, the control device 21 determines whether the sample rack type needs to be set. If the sample rack type needs to be set (step S2: yes), the process proceeds to step S3. If the sample rack type needs not to be set (step S2: no), the process proceeds to step S5.

[0145] Next, in step S3, the display unit 31 displays the sample rack type setting interface (refer to Fig. 22 ), the user enters the sample rack type setting interface to set the sample rack information. In the first column of the interface, the number of the sample rack 80 can be set, and in the second column, the sample rack 80 with the corresponding number can be set as a micro whole blood sample rack or a pre-diluted blood sample rack. At most, only one of the two can be selected. If the micro whole blood sample rack or the pre-diluted blood sample rack is not checked, or the sample rack 80 with a number is not set in the interface, the blood sample analyzer 1 will treat it as a normal blood sample rack. That is, when the micro whole blood sample rack or the pre-diluted blood sample rack is checked, the sample containers on the sample rack 80 with the corresponding number will be treated as the first sample container 91 by the blood sample analyzer 1; if the micro whole blood sample rack or the pre-diluted blood sample rack is not checked, or the sample containers on the sample rack 80 with a number is not set in the interface, the blood sample analyzer 1 will treat them as the second sample container 92.

[0146] When the second column is checked for micro whole blood (first measurement mode), the sample aspirating needle 135 of the blood analyzer 1 aspirates a first sampling amount of blood sample from the first sample container 91 on the sample rack 80 with the corresponding number, for example, preferably 5-50 μL, more preferably 15-35 μL, and most commonly 30 μL; when the second column is checked for pre-dilution (third measurement mode), the sample aspirating needle of the blood analyzer 1 aspirates a third sampling amount of blood sample from the first sample container 91 on the sample rack 80 with the corresponding number, for example, 80 μL; when the micro whole blood sample rack or pre-dilution blood sample rack (second measurement mode) is not checked, the sample aspirating needle of the blood analyzer 1 aspirates a second sampling amount of blood sample from the second sample container 92 on the sample rack 80 with the corresponding number, for example, 50-300 μL, and most commonly 70 μL. Preferably, the first sampling amount is smaller than the second sampling amount.

[0147] Fig. 22 This is the setting interface of the blood analyzer 1. Fig. 22 As shown, the user can call the setting interface through the display component 31 to set certain numbered sample racks as special sample racks for micro-blood collection tubes. Fig. 22 The blood analyzer 1 will treat the sample rack with the number entered in the interface as a special sample rack for micro-blood collection tubes. During mixing, the sample container 91 fixed on the special sample rack for micro-blood collection tubes will be transported to the first mixing device 11 by the transport device for mixing (detailed description will be given later). Fig. 22 For sample racks without input numbers in the interface shown, the blood analyzer 1 will treat them as common venous blood collection tube sample racks, and the second mixing device 12 will be used to grab the sample containers 92 on the sample rack for mixing.

[0148] In such Fig. 22 In the analyzer setting interface shown in the figure, the user can also set the type of micro-blood collection tube fixed by the micro-blood collection tube dedicated sample rack (refer to Fig. 22 The third column has several most common micro-tube types pre-set in the software for selection) and the sample volume in the micro-tube (refer to Fig.21 Fourth column), the control device 21 of the blood analyzer 1 automatically selects the rotation speed of the motor 111 of the first mixing device 11 for driving the sample stirring component 112 according to the user's settings, wherein the relationship between the type and size of the micro-blood collection tube and the motor rotation speed has been pre-set in the software program.

[0149] In such Fig. 22 In the blood analyzer setting interface shown, if the user sets certain numbered sample racks as special sample racks for micro-blood collection tubes, but does not set the corresponding micro-blood collection tube type or sample volume, the control device 21 of the blood analyzer 1 sets the speed of the motor 111 of the first mixing device 11 for driving the sample stirring component 112 to the default speed for mixing the sample containers on the sample rack with that number.

[0150] like Fig. 22 In the arrangement shown, for sample racks numbered 1 to 5, the blood analyzer 1 will use the first mixing device 11 to mix the sample containers on the sample racks, and for sample racks not numbered 1 to 5, the analyzer 1 will use the second mixing device 12 to mix the sample containers on the sample racks.

[0151] In one embodiment, the sample racks numbered 1-5 can be further distinguished, and sample containers of different volumes of blood samples, or sample containers of different shapes or sizes can be placed on the sample racks numbered 1-3, 4 and 5, respectively. For the sample containers on the sample racks numbered 1 to 3, the motor 111 of the first mixing device 11 uses a rotation speed of M1 revolutions / revolution for mixing; for the sample containers on the sample rack numbered 4, the motor 111 of the first mixing device 11 uses a default rotation speed of M0 revolutions / revolution for mixing; for the sample containers on the sample rack numbered 5, the motor 111 of the first mixing device 11 uses a rotation speed of M2 revolutions / revolution for mixing.

[0152] The blood analyzer 1 treats all the sample containers on the sample rack 80 numbered 1 to 5 as the first sample container 91, and treats all the sample containers on the sample racks not numbered 1 to 5 as the second sample container 94. When the sample aspirating needle of the blood analyzer 1 aspirates the sample from the sample container 91 on the sample racks numbered 1 to 4, it aspirates the first sampling amount of blood sample (micro whole blood sample); when the sample aspirating needle of the blood analyzer 1 aspirates the sample from the sample container 91 on the sample rack 80 numbered 5, it aspirates the third sampling amount of blood sample (pre-diluted blood sample); when the sample aspirating needle of the blood analyzer 1 aspirates the sample from the sample container 92 on the sample rack 80 not numbered 1 to 5, it aspirates the second sampling amount of blood sample (normal blood sample).

[0153] return Fig. 20 In step S4, the sample rack information is stored, and then the process proceeds to step S5. In step S5, the user selects a sample injection mode.

[0154] In step S6, the control device 21 determines whether it is the first injection mode. If it is determined to be the first injection mode (step S6: Yes), the control device 21 determines whether the start button (not shown) is pressed (step S7). If the control device 21 determines that the start button is not pressed (step S7: No), the process proceeds to step S25. If it is determined that the start button has been pressed (step S7: Yes), the process proceeds to step S8.

[0155] In step S8, the sample transport device 17 transports the sample containers 91 (92) on the sample rack 80 to the scanning position (not shown) one by one, and the container rotating scanning device (including the container clamping component 14, the container rotating component 15, and the scanner 16) reads the sample coding information on the label 911 (921) of the sample container 91 (92), and scans the sample rack 80 passing through the scanning position to read the coding information of the label of the sample rack 80 (step S9).

[0156] The control device 21 controls the sample transport device 17 to transport the sample containers 91 ( 92 ) on the sample rack 80 to the predetermined position 22 one by one (step S10 ).

[0157] The control device 21 controls the first mixing device 11 or the second mixing device 12 to mix the blood sample in the sample container 91 (92) (step S11). In step S11, the control device 21 determines whether the current measurement mode is the first measurement mode, the second measurement mode or the third measurement mode based on the coded information read from the label of the sample rack 80. If the control device 21 determines that the current measurement mode is the first measurement mode or the third measurement mode, the first mixing device 11 is moved to stir and mix the blood sample in the sample container 91 on the sample rack 80 located at the above-mentioned predetermined position 22 (the first mixing position); if the control device 21 determines that the current measurement mode is the second measurement mode, the clamping jaw 1201 of the second mixing device 12 is controlled to grab the current sample container 92 from the sample rack 80 located at the above-mentioned predetermined position to a certain position (the second mixing position) (not shown), and the stepping motor 1223 of the second mixing device 12 is controlled to drive the clamping jaw 1201 to rotate, thereby mixing the blood sample in the current sample container. In other embodiments, the first mixing position can be set on the sample rack, that is, the hole position on the sample rack serves as the first mixing position, and the stirring part moves to this position to mix the sample in the test tube located in the first mixing position; or the first mixing position is a fixed position independently set relative to the sample rack, so as to better fix the test tube during the stirring operation. When the first mixing position is a fixed position independently set relative to the sample rack, a transport device can be additionally provided to grab the first sample container and transport it to the first mixing position, and the grabbing mechanism of the second mixing device can also be used as a transport device to transport the first sample container. When the first mixing position can be set on the sample rack, preferably, as Fig.23 As shown, the control device 21 controls the container clamping assembly 14 to move so that the two driven wheels 144a and 144b clamp the sample container 91 (92) on the sample rack 80, and controls the sample stirring component 112 of the first mixing device 11 to move downward (in the Z direction) into the blood sample in the sample container 91 (92) to mix the blood sample.

[0158] In this embodiment, in step S11, the clamp 1201 of the second mixing device 12 can also grab the sample container 91 (92) from the sample rack 80 at the above-mentioned predetermined position 22 and move it to the above-mentioned certain position, and then move the first mixing device 11 so that the sample stirring component 112 of the first mixing device 11 enters the sample container 91 grabbed by the clamp 1201 to perform stirring and mixing.

[0159] In this embodiment, preferably, Fig.23As shown, in step S11, when the control device 21 determines that the sample container on the current sample rack 80 is the sample container 91 containing a trace whole blood sample (or a pre-diluted trace blood sample) according to the coding information of the label of the sample rack 80, the container clamping assembly 14 is controlled to move so that the two driven wheels 144a and 144b clamp the sample container 91 on the sample rack 80, and the sample stirring component 112 of the first mixing device 11 is controlled to enter the blood sample in the sample container 91 downward (in the Z direction) to mix the blood sample; when the control device 21 determines that the sample container on the current sample rack 80 is the sample container 92 containing a normal blood sample according to the coding information of the label of the sample rack 80, the second mixing device 12 is controlled to drive the clamping claw 1201 to grab the sample container 92 from the sample rack 80 to a certain position and rotate it, so as to mix the blood sample in the sample container 92 (for example, by inverting and mixing).

[0160] In this embodiment, the control device 21 can also control the movement of the container clamping assembly 14 so that the two driven wheels 144a and 144b clamp the sample container 91 on the sample rack 80 and move it to a certain position, and control the sample stirring component 112 of the first mixing device 11 to enter the blood sample in the sample container 91 downward (in the Z direction) for mixing.

[0161] The sample container 91 (92) containing the mixed blood sample is transported to the first sampling position (not shown) of the blood sample analyzer (step S12), and then the process proceeds to step S13.

[0162] In step S13, the control device 21 controls the sample aspirating needle 135 of the sample aspirating device 13 to aspirate a predetermined amount of blood sample from the sample container 91 (92) at the sampling position according to the received measurement mode information. Specifically, in the first measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a first sampling amount of blood sample from the first sample container 91; in the third measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a third sampling amount of blood sample from the first sample container 91; in the second measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a second sampling amount of blood sample from the second sample container 92.

[0163] In step S14, the sample preparation device of the blood sample analyzer 1 prepares a test sample from the blood sample aspirated by the sample aspirating device 13. In the first measurement mode, the aspirated first sample volume of the blood sample is used to prepare the first test sample; in the third measurement mode, the aspirated third sample volume of the pre-diluted blood sample is used to prepare the third test sample; in the second measurement mode, the aspirated second sample volume of the blood sample is used to prepare the second test sample.

[0164] In step S15, the detector of the blood sample analyzer 1 detects the test sample prepared by the sample preparation device and obtains the test result. The control device 21 determines whether there is an unprocessed next sample container 91 (92) on the sample rack (step S16). If there is an unprocessed sample container 91 (92) (step S16: yes), return to step S8 for corresponding processing. If all sample containers 91 (92) have been processed (step S16: no), end the first injection mode (step S17) and enter step S26.

[0165] If it is determined to be the second injection mode (step S6: No), the sample compartment cover 181 is opened (step S18). Regarding step S18, when the control device 21 is in the second injection mode, if the sample compartment cover 181 is originally in a closed state, this step S18 is executed, and if the sample compartment cover 181 is originally in an open state, the next step S19 is directly entered.

[0166] In step S19 , the user selects a measurement mode for the current blood sample through the setting interface of the blood analyzer 1 .

[0167] The control device 21 determines whether the start button (not shown) is pressed (step S20). If the control device 21 determines that the start button is not pressed (step S20: No), the process proceeds to step S26. If the control device 21 determines that the start button is pressed (step S20: Yes), the process proceeds to step S21, closes the sample compartment cover 181, and proceeds to step S22.

[0168] In step S22, the control device 21 controls the sample aspirating needle 135 of the sample aspirating device 13 to aspirate a predetermined amount of blood sample from the sample container 91 (92) on the second sampling position according to the measurement mode information selected by the user in step S19. Specifically, in the first measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a first sampling amount of blood sample from the first sample container 91; in the third measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a third sampling amount of pre-diluted blood sample from the first sample container 91; in the second measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a second sampling amount of blood sample from the second sample container 92. Preferably, the first sampling amount is smaller than the second sampling amount, for example, the first sampling amount is 5-50 μL, and more preferably 15-35 μL.

[0169] In step S23, the sample preparation device of the blood sample analyzer 1 prepares a test sample from the blood sample aspirated by the sample aspirating device 13. In the first measurement mode, the aspirated first sample volume of the blood sample is used to prepare the first test sample; in the third measurement mode, the aspirated third sample volume of the pre-diluted blood sample is used to prepare the third test sample; in the second measurement mode, the aspirated second sample volume of the blood sample is used to prepare the second test sample.

[0170] In step S24, the detector of the blood sample analyzer 1 detects the test sample prepared by the sample preparation device to obtain the test result, and then ends the second sample injection mode (step S25) and enters step S26.

[0171] In step S26, if no shutdown instruction is received (step S26: No), return to step S2; if a shutdown instruction is received (step S26: Yes), execute shutdown (step S27) and end the processing.

[0172] In the above embodiment, it can be used to perform blood analysis on major examination items such as red blood cells, white blood cells and platelets in a routine blood test, and in step S15, the detection sample prepared by the sample preparation device is detected by the detector to obtain relevant indicators involving major examination items such as red blood cells, white blood cells and platelets, such as white blood cell count (WBC), red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MVHC), platelet count (PLT), lymphocyte ratio (LY%), monocyte ratio (MONO), neutrophil ratio (NEUT%), lymphocyte (LY), monocyte count (MONO), neutrophil count (NEUT), red blood cell distribution width (RDW), platelet volume distribution width (PDW), mean platelet volume (MPV) and / or large platelet ratio (P-LCR) and other indicators.

[0173] Fig.24 The schematic flow chart of the mixing process in step S11 is shown. Fig.24 As shown, the control device 21 compares the coding information read from the label of the sample rack 80 with the user's preset sample rack information (step S1101), and determines whether the read coding information matches the preset sample rack information (step S1102). If the read coding information matches the preset sample rack information (step S1102: yes), it is determined that the sample on the current sample rack performs the first or third measurement mode, and the preset mixing parameters are called to set the first mixing device 11 (step S1103), and then the control device 21 controls the first mixing device 11 to mix the blood sample on the current sample container 91 used for the first measurement mode or the third measurement mode (step S1104). If the read coding information does not match the preset sample rack information (step S1102: no), it is determined that the sample on the current sample rack performs the second measurement mode, and the control device 21 controls the second mixing device 12 to mix the blood sample on the current sample container 92 (step S1105).

[0174] In step S1104 of this embodiment, after the first mixing device 11 drives the sample stirring component 112 to complete the mixing operation of the blood sample in the sample container 91, the sample stirring component 112 is cleaned and dried. Preferably, a cleaning swab can be used to clean and air dry the sample stirring component 112 (refer to Figure 5 In this embodiment, the swab may be fixed and the sample stirring component 112 may be moved for cleaning and air drying, or the sample stirring component 112 may be fixed and the swab may be moved for cleaning and air drying.

[0175] In this embodiment, the cleaning component may also include a cleaning pool 114. In step S1104, cleaning may also be performed by a cleaning pool (see Fig.25 That is, after the sample stirring component 112 completes stirring, it automatically moves into the cleaning pool 114, and the sample stirring component 112 is cleaned by flushing with the liquid in the cleaning pool 114 or the like.

[0176] Fig.26 FIG. 2 is an example diagram of the sample aspirating process in step S13 in the first injection mode of this embodiment. Fig.26 As shown, the coding information of the sample rack 80 label read by the container rotating code scanning device is compared with the sample rack information preset by the user (step S131). Based on the comparison result, it is determined whether the current sample rack 80 is a micro whole blood sample rack (step S132). If it is a micro whole blood sample rack (step S132: yes), the measurement mode of the device is set to the first measurement mode (step S133), and then the sample aspirating device 13 aspirates the first sampling amount of blood sample from the sample container 91 on the sample rack 80 (step S134).

[0177] If it is not a micro whole blood sample rack (step S132: No), it is determined whether it is a pre-dilution micro blood sample rack (step S135). If it is a pre-dilution micro blood sample rack (step S135: Yes), the measurement mode of the device is set to the third measurement mode (step S136), and then the sample aspirating device 13 aspirates the third sampling amount of blood sample from the sample container 91 on the sample rack 80 (step S137).

[0178] If it is not a pre-diluted micro blood sample rack (step S135: No), the measuring mode of the device is set to the second measuring mode (step S138), and then the sample aspirating device 13 aspirates the second sampling amount of blood sample from the sample container 92 on the sample rack 80 (step S139).

[0179] Fig. 27 FIG. 2 is an example diagram of the sample aspirating process in step S22 in the second injection mode of this embodiment. Fig. 27As shown, it is determined whether the measurement mode selected by the user is the first measurement mode (step S221). If it is the first measurement mode (step S221: yes), the sample suction device 13 sucks the first sampling amount of blood sample from the sample container 91 (92, 93) on the sample rack 80 (step S222). If it is not the first measurement mode (step S221: no), it is determined whether it is the second measurement mode (step S223). If it is the second measurement mode (step S223: yes), the sample suction device 13 sucks the second sampling amount of blood sample from the sample container 94 on the sample rack 80 (step S224). If it is not the second measurement mode (step S223: no), the sample suction device 13 sucks the third sampling amount of blood sample from the sample container 91 (92, 93) on the sample rack 80 (step S225).

[0180] (Implementation Method 2)

[0181] The structure of the blood analyzer in this embodiment differs from that of the blood analyzer 1 in Embodiment 1 in that the blood analyzer in this embodiment does not have the second mixing device 12, and the remaining parts have the same structure as the corresponding parts in the blood analyzer 1 in Embodiment 1, so the same reference numerals are used for the same structural parts and the description is omitted.

[0182] Fig.28 and Fig.29 The main flow of an example of blood sample analysis and processing by the blood sample analyzer is shown. Steps S421 to S430 and S432 to S447 are the same as the processing operations of steps S1 to S10 and S12 to S27 in the first embodiment, and their description is omitted here.

[0183] In step S431 , the control device 21 controls the first mixing device 11 to move to above the current sample container 91 for the first measurement mode or the third measurement mode, then lowers the sample stirring component 112 of the first mixing device 11 , and drives the sample stirring component 112 to mix the blood sample in the sample container 91 .

[0184] Preferably, if Fig.23 As shown, the control device 21 controls the container clamping assembly 14 to move so that the two driven wheels 144a and 144b clamp the sample container 91 (92) on the sample rack 80, and controls the sample stirring component 112 of the first mixing device 11 to move downward (in the Z direction) into the blood sample in the sample container 91 (92) to mix the blood sample.

[0185] In this embodiment, the blood analyzer has only the first mixing device 11. The first mixing device 11 can be used to mix a trace whole blood sample, a pre-diluted blood sample or a venous blood sample, and the container cover of the sample container 92 on the sample rack 80 is opened before the sample transport device 17 transports the sample rack 80. Preferably, the first mixing device 11 is only used to mix a trace whole blood sample and a pre-diluted blood sample.

[0186] (Implementation 3)

[0187] The structure of the blood analyzer in this embodiment is different from that of the blood analyzer in Embodiment 2 in that the blood analyzer in this embodiment is only provided with the second sample injection mode, but not provided with the sample transport device 17, that is, not provided with the first sample injection mode, so that the blood analyzer is more compact. The remaining parts are the same as the corresponding parts of the blood analyzer in Embodiment 2, so the same reference numerals are used for the same structural parts and the description is omitted.

[0188] Fig.30 The main flow chart of an example of blood sample analysis by blood sample analyzer 1 is shown in FIG. Fig.30 As shown, firstly, the power of the blood sample analyzer 1 is turned on, and the control device 21 starts initialization (step S501). In this initialization step, the program is initialized, the liquid circuit components of the blood sample analyzer 1 are initialized, the pipeline is cleaned, and the driving part is reset.

[0189] Next, in step S502, the measurement mode is selected on the setting interface displayed on the display unit 31. The control device 21 determines whether the start button (not shown) is pressed (step S503). If the control device 21 determines that the start button is not pressed (step S503: No), the process proceeds to step S510. If the start button is determined to be pressed (step S503: Yes), the sample compartment cover 181 is closed (step S504). With regard to step S504, when the sample compartment cover 181 is originally in a closed state, the process directly proceeds to the next step S505. If the sample compartment cover 181 is originally in an open state, the process executes step S504.

[0190] The control device 21 controls the first mixing device 11 or the second mixing device 12 to mix the blood sample in the sample container 91 (92) (step S505). In step S505, the control device 21 determines whether the current measurement mode is the first measurement mode, the second measurement mode, or the third measurement mode. If the control device 21 determines that the current measurement mode is the first measurement mode or the third measurement mode, the control device 21 controls the first mixing device 11 to mix the blood sample in the sample container 91 on the sample container fixing hole 182.

[0191] In step S505, if the control device 21 determines that the current measurement mode is the second measurement mode, the stepper motor 1223 of the second mixing device 12 is controlled to drive the clamping claw 1201 to grab the sample container 92 from the sample container fixing hole 18 to a certain position and rotate it, thereby mixing the blood sample of the current sample container 92. Those skilled in the art will appreciate that the second mixing device may not be provided, and only a trace amount of whole blood or a pre-diluted blood sample may be detected, so as to further miniaturize the blood analyzer.

[0192] In step S506, the control device 21 controls the sample aspirating needle 135 of the sample aspirating device 13 to aspirate a predetermined amount of blood sample from the sample container 91 (92) at the sampling position according to the measurement mode information selected by the user. Specifically, in the first measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a first sampling amount of blood sample from the first sample container 91; in the third measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a third sampling amount of blood sample from the first sample container 91; in the second measurement mode, the sample aspirating needle 135 of the sample aspirating device 13 aspirates a second sampling amount of blood sample from the second sample container 92. Preferably, the first sampling amount in the first measurement mode is less than the second sampling amount in the second mode, for example, the first sampling amount is preferably 5-50 μL, more preferably 15-35 μL.

[0193] After the blood sample aspiration process is completed, the sample chamber cover 181 is opened to take out the aspirated sample container 91 (92) (step S507). In the present application, the step of opening the sample chamber cover 181 and taking out the aspirated sample container 91 (92) can also be performed at any time after the blood sample aspiration process is completed, and is not limited to taking out the blood sample immediately after the blood sample aspiration process is completed.

[0194] In step S508, the sample preparation device of the blood sample analyzer 1 prepares a test sample from the blood sample aspirated by the sample aspirating device 13. In the first measurement mode, the first test sample is prepared from the aspirated first sample volume of the blood sample; in the third measurement mode, the third test sample is prepared from the aspirated third sample volume of the pre-diluted blood sample; in the second measurement mode, the second test sample is prepared from the aspirated second sample volume of the blood sample. Preferably, the first sample volume is smaller than the second sample volume, for example, the first sample volume is 5-50 μL, more preferably 15-35 μL.

[0195] In step S509, the detector of the blood sample analyzer 1 detects the test sample prepared by the sample preparation device, obtains the test result, and enters step S510.

[0196] In step S510, if no shutdown instruction is received (step S510: No), return to step S502; if a shutdown instruction is received (step S510: Yes), execute shutdown (step S511) and end the processing.

[0197] In this embodiment, the sample in the sample container 91 mixed by the first mixing device 11 may be a whole blood sample or a pre-diluted sample. The whole blood sample may be a peripheral whole blood sample or a venous whole blood sample.

[0198] In this embodiment, preferably, the first mixing device 11 performs mixing operation only on a trace amount of whole blood sample or a pre-diluted blood sample.

[0199] Blood is composed of blood cells and plasma. Since the specific gravity of blood cells is greater than that of plasma, blood will be stratified after being left to stand for a period of time, with blood cells sinking to the bottom and plasma at the top. One parameter of blood sample measurement is hemoglobin concentration (HGB), which refers to the amount of hemoglobin contained in a unit volume of blood. Hemoglobin, also known as hemoglobin, only exists in red blood cells and is the main component of red blood cells.

[0200] When the blood sample is not fully mixed, the red blood cell concentration in the lower part of the blood sample is higher than that in the upper part. When the sampling needle draws the sample near the bottom of the blood collection tube (in order to reduce the blood collection volume requirements of the blood analyzer, the sampling needle will draw the sample near the bottom of the blood collection tube), the hemoglobin concentration (HGB) measured by the blood analyzer will be significantly higher than the actual value, and the fluctuation range of the hemoglobin concentration (HGB) measured multiple times will be large. Therefore, the stability of the hemoglobin concentration (HGB) measurement is often used to measure the effect of blood sample mixing.

[0201] In the above-mentioned embodiments, not only the problem of blood sample loss and peripheral blood mixing caused by blood adhering to the blood collection tube cap or tube wall is avoided, but also the blood sample can be fully stirred and evenly mixed. When the blood sample is fully mixed, its hemoglobin concentration (HGB) is a very stable parameter, and the fluctuation range of repeated measurements generally does not exceed ±2g / L.

[0202] In the above embodiment, the sample stirring component 112 of the first mixing device 11 is driven by the mixing device driving motor to enter the sample container for stirring. The present application is not limited to this, and the sample transport device 17 can also transport the sample rack 80 containing the sample container to a predetermined position, and the clamping claw 1201 of the second mixing device 12 can grasp and move the sample rack 80 or the sample container on the sample rack 80 to the mixing position, so that the sample stirring component 112 relatively enters the sample container without moving the first mixing device 11, and the sample stirring component 112 is driven to stir the blood sample in the sample container. In this way, the blood sample in the sample container is stirred, so that the moving device of the first mixing device 11 can be omitted.

[0203] In the above embodiment, the sample stirring component 112 can also be installed on a mechanism that can move up and down, and the stirring rod can be controlled to move up and down by a motor drive, a pulley or a screw drive. At the same time, the sample stirring component 112 is installed in the up and down moving mechanism and connected by a bearing, so that the sample stirring component 112 can rotate along its own axis while moving up and down. The above-mentioned sample container 91 (92) is placed on the sample rack 80 and is moved horizontally to a predetermined position (mixing position) by the sample transport device 17. After the sample container 91 (92) arrives, the sample stirring component 112 moves downward (such as Fig.23 The sample stirring component 112 extends in the Z direction of the sample container 91 (92) and reaches the bottom of the sample container 91 (92). After the sample stirring component 112 is in place, the sample inside the sample container 91 (92) is driven to rotate by rotating along the axis of the sample stirring component 112 itself, thereby achieving the effect of mixing the sample. After the mixing is completed, the sample stirring component 112 rises and at the same time, a small amount of sample adhering to the outer wall of the sample stirring component 112 is cleaned by the cleaning component 113. When the sample stirring component 112 leaves the sample container 91 (92), the sample transport device 17 pushes the sample container 91 (92) to move, so that the sample container 91 (92) reaches the blood collection position, and the analyzer starts to collect blood and analyze.

[0204] The present application can be used to measure hemoglobin concentration (HGB). Hemoglobin concentration (HGB) is an important parameter for blood sample measurement, which refers to the amount of hemoglobin contained in a unit volume of blood. Hemoglobin, also known as hemoglobin, only exists in red blood cells and is the main component of red blood cells. Blood is composed of blood cells and plasma. Since the specific gravity of blood cells is greater than that of plasma, blood will be stratified after standing for a period of time, with blood cells sinking to the bottom and plasma at the top.

[0205] When the blood sample is not fully mixed, the red blood cell concentration in the lower part of the blood sample is higher than that in the upper part. When the sampling needle draws the sample near the bottom of the blood collection tube (in order to reduce the blood collection volume requirements of the blood analyzer, the sampling needle will draw the sample near the bottom of the blood collection tube), the hemoglobin concentration (HGB) measured by the blood analyzer will be significantly higher than the actual value, and the fluctuation range of the hemoglobin concentration (HGB) measured multiple times will be large. Therefore, the stability of the hemoglobin concentration (HGB) measurement is often used to measure the effect of blood sample mixing.

[0206] In the present application, a test sample for hemoglobin concentration (HGB) test item is prepared from a blood sample of a test subject by a sample preparation device, and an index related to hemoglobin concentration (HGB) is obtained by a detector.

[0207] In the above embodiments, not only the blood sample loss and peripheral blood mixing problems caused by blood adhering to the blood collection tube cap or tube wall are avoided, but also the blood sample can be fully mixed. When the blood sample is fully mixed, its hemoglobin concentration (HGB) is a very stable parameter, and the fluctuation range of repeated measurements generally does not exceed ±2g / L. Repeated measurements here refer to more than two measurements.

[0208] Fig.31 The following is a diagram showing the analysis data of 6 micro-blood samples, each containing 100 μL of whole blood. The samples were mixed by the mixing device 11 and measured 6 times using the first measurement mode. Fig.31 According to the data, the fluctuation range of hemoglobin concentration (HGB) is only 1g / L, which is very stable.

[0209] Fig.32 There are six first sample containers 91 containing different trace amounts of whole blood, namely 30 μL, 50 μL, 100 μL, 150 μL, 200 μL and 250 μL respectively. The blood is mixed by the mixing device 11 and the HGB data are measured six times using the first measurement mode. It can be seen from the data that the fluctuation range of the hemoglobin concentration (HGB) does not exceed ±2 g / L, which meets the measurement requirements.

[0210] In the above-mentioned embodiment 1, the second mixing device 12 can grab the sample container on the sample rack 80 and drive the sample container containing the normal blood to be inverted and mixed. However, the present application is not limited to this, and the second mixing device 12 can also grab the sample rack 80 and drive all the sample containers containing the normal blood on the sample rack 80 to be inverted and mixed.

[0211] In the present application, the mixing position refers to the position where the first mixing device 11 or the second mixing device 12 mixes the blood sample in the sample container. For example, when the first mixing device 11 mixes the blood sample in the sample container at the predetermined position, the first mixing position and the predetermined position are the same position.

[0212] (Implementation 4)

[0213] Fig.33 FIG. 1 is a schematic diagram of the structure of the mixing device of this embodiment. Fig.33 As shown, the sample transport device 17 or the sample chamber assembly 18 transports the sample container 91 (92) to the sampling position. The so-called sampling position refers to the position where the sampling needle 205 (135) performs sampling. The sample suction device 13 moves the sample suction needle 205 into the sample container 91 (92), and drives the sample suction needle 205 to suck a proper amount of blood sample, and then returns the sucked blood sample to the sample container 91 (92), so that the blood sample in the sample container 91 (92) forms a certain flow, and the blood sample is mixed.

[0214] Fig.34 FIG. 2 is a schematic diagram of the structure of the sample suction device of this embodiment. Fig.34 As shown, the sample suction device 20 is used to mix the blood sample in the sample container 91 (92) sent to the sampling position of the analyzer 1 by the sample transport device 17 and to absorb an appropriate amount of blood sample from the mixed blood sample for sample preparation.

[0215] The sample suction device 20 includes: a sample suction needle 205, a sample suction needle moving assembly 201, a stepper motor 2001, synchronous wheels 2002 and 2003, an annular synchronous toothed belt 2004 wound around the synchronous wheels 2002 and 2003, a linear guide rod 2005 placed along the Y1 and Y2 directions, a position sensor 2006, an aspiration drive device (not shown), a sample suction needle air drying device (not shown), etc. The aspiration drive device is used to drive the sample suction needle 205 to aspirate a proper amount of blood sample, and then return the aspirated blood sample to the sample container 91 (92), so that the blood sample in the sample container 91 (92) forms a certain flow and mixes the blood sample. Preferably, the aspiration drive device is a syringe.

[0216] The sample suction needle moving assembly 201 is connected to the annular synchronous toothed belt 2004 through a connecting piece. The annular synchronous toothed belt 2004 is driven by the rotation of the stepping motor 2001 and rotates under the guidance of the two synchronous wheels 2002 and 2003. The sample suction needle moving assembly 201 can drive the sample suction needle 205 to move along the Y1 or Y2 direction under the drive of the stepping motor 2001. The initial position of the sample suction needle moving assembly 201 in the Y1 and Y2 directions is positioned by the position sensor 2006 and the sensor sensing sheet 2018 fixed to the sample suction needle moving assembly 201.

[0217] The sample aspirating needle moving assembly 201 includes: a stepper motor 2011, a screw rod 2012, a nut 2023, a linear guide rail 2014, a sample aspirating needle fixing component 2015, a position sensor 2016, a sensor sensing sheet 2017, a sample aspirating needle position sensor (not shown), etc. Among them, the sample aspirating needle position sensor is used to sense the downward position of the sample aspirating needle 205 to prevent the needle tip of the sample aspirating needle 205 from continuing to move downward after reaching the bottom of the sample container 91 (92), thereby causing damage to the needle tip of the sample aspirating needle 205 or the sample container 91 (92).

[0218] The sample suction needle 205 is fixed on the sample suction needle fixing component 2015, and the sample suction needle fixing component 2015 is fixed on the linear slide rail 2014 placed along Z1 and Z2 by screws, and the nut 2013 is stuck in the slot provided in the sample suction needle fixing component 2015, and there is no relative rotation between the nut 2013 and the sample suction needle fixing component 2015. The lead screw 2012 is connected to the rotating shaft of the stepper motor 2011 by screws. The stepper motor 2011 can drive the screw 2012 to rotate, and drive the sample suction needle fixing component 2015 to move the sample suction needle 205 along the Z1 or Z2 direction. The initial position of the sample aspirating needle 205 in the Z1 and Z2 directions is positioned by the position sensor 2017 and the optical coupling sensor sensing sheet (not shown) arranged on the sample aspirating needle fixing component 2015, and the sample aspirating needle 205 is moved and positioned along the Z1 or Z2 direction by the sample aspirating needle position sensor to prevent the needle tip of the sample aspirating needle 205 from continuing to move downward after reaching the bottom of the sample container 91 (92).

[0219] Driven by the stepper motor 2001 and the stepper motor 2011, the sample aspirating needle 205 can move in two dimensions along the Y1, Y2 directions and the Z1, Z2 directions, so as to achieve the functions of mixing the blood sample in the sample container through the suction and exhalation action, sucking an appropriate amount of blood sample from the mixed blood sample, and dividing the blood sample into the sample preparation device.

[0220] In this embodiment, the steps of mixing the blood sample in the sample container 91 (92) by sucking and discharging the blood sample through the sample aspiration needle 205 are as follows:

[0221] The control device 21 determines whether the injection mode is the first injection mode or the second injection mode;

[0222] If it is the first sampling mode, the sample transport device 17 transports the sample container 91 (92) on the sample rack 80 to the first sampling position; if it is the second sampling mode, the sample chamber assembly 18 transports the single sample container 91 (92) to the second sampling position, wherein the first sampling position and the second sampling position may be the same position or different positions;

[0223] The outer wall of the sample suction needle 205 is dried by the sample suction needle air drying device, and the suction and exhalation driving device drives the sample suction needle 205 to absorb a proper amount of air, so that an isolation air column is formed inside the sample suction needle 205;

[0224] The sample aspirating needle moving component 201 drives the sample aspirating needle 205 downward, and the sample aspirating needle position sensor or the sample aspirating needle driving device determines whether the needle tip of the sample aspirating needle 205 reaches the bottom of the sample container 91 (92) according to the number of motor steps. If the needle tip of the sample aspirating needle 205 reaches the bottom of the sample container 91 (92), the sample aspirating needle moving component 201 stops driving the sample aspirating needle 205 downward, otherwise it continues to drive the sample aspirating needle 205 downward until it reaches the bottom of the sample container 91 (92);

[0225] After the suction and exhalation drive device drives the sample suction needle 205 to suck an appropriate amount of blood sample, the sucked blood sample is returned to the sample container 91 (92), so that the blood sample in the sample container 91 (92) forms a certain flow until the blood sample is mixed;

[0226] The sample aspirating needle 205 aspirates an appropriate amount of the mixed blood sample from the sample container 91 ( 92 ) to collect the blood sample.

[0227] In this embodiment, the sample container 91 (92) contains a whole blood sample, and since the whole blood sample is directly sucked out and mixed by the sampling needle 205, the sampling needle 205 can directly suck a predetermined volume of the whole blood sample after mixing, and there is no need to clean the sampling needle.

[0228] Those skilled in the art will appreciate that the various operations, methods, steps, measures, and schemes in the processes discussed in this application may be alternated, altered, combined, or deleted. Further, other steps, measures, and schemes in the various operations, methods, and processes discussed in this application may also be alternated, altered, rearranged, decomposed, combined, or deleted. Further, the steps, measures, and schemes in the prior art that are similar to those disclosed in this application may also be alternated, altered, rearranged, decomposed, combined, or deleted.

[0229] The above description is only a partial implementation method of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present application. These improvements and modifications should also be regarded as the scope of protection of the present application.

Claims

1. A blood sample analyzer, include: A sample chamber assembly, comprising a sample chamber cover and a sample container fixing hole, for performing single sample injection into a sample container containing a trace blood sample or a constant blood sample placed on the sample container fixing hole; A mixing device, comprising a sample stirring component for stirring the blood sample in the sample container, wherein the mixing device can drive the sample stirring component to move up and down, and the sample stirring component can move downward into the sample container to stir and mix the trace blood sample, and the mixing device can also invert the sample container to mix a constant amount of blood sample; A control device, in communication with the mixing device; A measurement mode setting device, used for setting a first measurement mode and a second measurement mode; Wherein, the control device performs the following actions according to the setting of the measurement mode setting device: (1) determining whether the first measurement mode or the second measurement mode is selected, wherein the first measurement mode is selected when the blood sample in the sample container is a trace blood sample, and the second measurement mode is selected when the blood sample in the sample container is a normal blood sample; (2) when it is determined that the first measurement mode is selected, controlling the mixing device to drive the sample stirring component to move into the sample container to stir and mix the trace blood sample; (3) When it is determined that the second measurement mode is selected, the mixing device is controlled to invert the sample container to perform inversion mixing.

2. The blood sample analyzer according to claim 1, Features: The blood sample analyzer is only used to process trace whole blood samples and pre-diluted blood samples.

3. The blood sample analyzer according to claim 1, further comprising: include: A sample suction device, used for sucking the mixed blood sample from the sample container; A sample preparation device, used for preparing the blood sample aspirated by the sample aspirating device into a test sample; The control device is in communication connection with the sample suction device and / or the sample preparation device to control the actions of the sample suction device and / or the sample preparation device.

4. The blood sample analyzer according to claim 3, further comprising: include: The control device performs the following actions according to the setting of the measurement mode setting device: When it is determined that the first measurement mode is selected, the sample aspirating device is controlled to aspirate a first sampling amount of the blood sample from the sample container on the sample rack, and the sample preparation device is controlled to prepare a first test sample; When it is determined that the second measurement mode is selected, the sample aspirating device is controlled to aspirate a second sample amount of the blood sample from the sample container on the sample rack, and the sample preparation device is controlled to prepare a second test sample; The first sampling amount is less than the second sampling amount.

5. The blood sample analyzer according to claim 4, Features: The first sampling volume is 5-50 μL.

6. The blood sample analyzer according to claim 5, Features: The first sample volume is 15-35 μL.

7. The blood sample analyzer according to claim 4, Features: The measurement mode setting device is also used to set a third measurement mode; When the control device determines that the third measurement mode is selected, the control device controls the sample aspirating device to aspirate a third sampling amount of the pre-diluted blood sample from the sample container on the sample rack, and controls the sample preparing device to prepare a third test sample.

8. A blood sample analyzer, include: A first mixing device, capable of mixing the trace blood sample in the first sample container, wherein the first mixing device has a sample stirring component for stirring the trace blood sample in the first sample container, wherein the sample stirring component can move up and down, and can move downward into the first sample container to stir and mix the trace blood sample; A second mixing device, capable of mixing the constant blood sample in the second sample container by inversion mixing; A control device is connected to the first mixing device and the second mixing device in communication, and is capable of performing the following operations: (1) determining whether it is the first measurement mode or the second measurement mode; (2) when it is determined that the first measurement mode is selected, controlling the first mixing device to mix the trace blood sample in the first sample container; (3) when it is determined that the second measurement mode is selected, controlling the second mixing device to mix the constant blood sample in the second sample container; A sample chamber assembly, comprising a sample chamber cover and a sample container fixing hole, for performing single sample injection into a sample container containing a trace blood sample or a constant blood sample placed on the sample container fixing hole; A sample transport device, used for transporting a sample rack containing a first sample container and / or a second sample container; The control device is also used to determine whether the current injection mode is the first injection mode or the second injection mode; When it is determined that the first sample injection mode is selected, the sample transport device is controlled to transport the sample rack containing the first sample container and / or the second sample container; When it is determined to be the second sample injection mode, the sample compartment assembly is controlled to transport a single sample container to the blood sample analyzer.

9. The blood sample analyzer according to claim 8, It is characterized in that The control device can also perform the following tasks: (1) Determine whether it is the third measurement mode; (2) When the control device determines that the third measurement mode is selected, the control device controls the first mixing device to mix the pre-diluted blood sample in the sample container.

10. The blood sample analyzer according to claim 8, It is characterized in that The trace blood sample in the first sample container is a whole blood sample, and the volume of the blood sample in the first sample container is 30-250 μl.

11. The blood sample analyzer according to claim 10, It is characterized in that The volume of the blood sample in the first sample container is 50-200 μl.

12. The blood sample analyzer according to claim 11, It is characterized in that The volume of the blood sample in the first sample container is 50-100 μl.

13. The blood sample analyzer according to claim 8, Features: The sample transport device can transport the sample rack containing the first and / or second sample containers to the first mixing position, or the sample transport device can transport the sample rack containing the first sample container to a predetermined position, the clamping claw of the second mixing device can grab the first sample container from the sample rack at the predetermined position to the first mixing position, and the first mixing device drives the sample stirring component to stir and mix the blood sample in the first sample container containing a trace blood sample located at the first mixing position.

14. The blood sample analyzer according to claim 8, Features: The sample transport device transports the sample rack containing the first sample container to a predetermined position, and the clamping claws of the second mixing device can grab the first sample container from the sample rack at the predetermined position to the second mixing position for inversion mixing.

15. The blood sample analyzer according to claim 8, Features: The head of the sample stirring component is cylindrical, paddle-shaped or polygonal.

16. The blood sample analyzer according to claim 8, Features: The control device controls the sample stirring component to stir in one or a combination of the following modes: rotation, circular orbit, linear swing or up and down oscillation.

17. The blood sample analyzer according to claim 8, Features: Also includes: A cleaning component is used to clean the sample stirring component.

18. The blood sample analyzer according to claim 17, Features: The cleaning component comprises a cleaning liquid inlet and a cleaning liquid outlet to clean the sample stirring component located in the cleaning component; the cleaning liquid outlet can also be used for exhausting air to dry the sample stirring component.

19. The blood sample analyzer according to claim 17, It is characterized in that The cleaning component includes a cleaning pool capable of cleaning the sample stirring component.

20. The blood sample analyzer according to claim 8, further comprising: include: A sample suction device, used for sucking the mixed blood sample from the sample container; When it is determined that the first measurement mode is selected, the control device controls the sample aspirating device to aspirate a first blood sample volume from the first sample container; When it is determined that the second measurement mode is selected, the control device controls the sample aspirating device to aspirate a second blood sample volume from the second sample container; The first sampling amount is less than the second sampling amount.

21. The blood sample analyzer according to claim 20, Features: The first sampling volume is 5-50 μL.

22. The blood sample analyzer according to claim 21, Features: The first sampling volume is 5-50 μL.

23. The blood sample analyzer according to claim 8, Features: The measurement mode setting device is also used to set a third measurement mode; When the control device determines that the third measurement mode is selected, the control device controls the first mixing device to mix the pre-diluted blood sample in the first sample container.

24. The blood sample analyzer according to any one of claims 8 to 23, Features: The first mixing device mixes the blood sample in the first sample container. The blood sample in the first sample container is a whole blood sample. The blood sample in the first sample container is 30-250 μL.

25. The blood sample analyzer according to claim 24, Features: The blood sample in the first sample container is 50-200 μL.

26. The blood sample analyzer according to claim 25, Features: The blood sample in the first sample container is 50-100 μL.

27. The blood sample analyzer according to any one of claims 8 to 23, Features: After the first mixing device mixes the trace blood sample in the first sample container, the fluctuation range of the hemoglobin value of the trace blood sample measured repeatedly for multiple times does not exceed ±2g / L.

28. A blood sample analyzer, include: A sample transport device, used to transport a sample rack equipped with a sample container, wherein the sample container can hold a trace amount of blood sample or a constant amount of blood sample; A mixing device, comprising a sample stirring component for stirring the blood sample in the sample container, wherein the mixing device can drive the sample stirring component to move up and down, the sample stirring component can move downward into the sample container to mix the trace amount of blood sample in the sample container, and can invert the sample container to mix a constant amount of blood sample; A control device, which is in communication with the sample transport device and the mixing device, and controls the actions of the sample transport device and the mixing device; A measurement mode setting device, used for setting a first measurement mode and a second measurement mode; The control device can also perform the following tasks: (1) determining whether the first measurement mode or the second measurement mode is selected, wherein the first measurement mode is selected when the blood sample in the sample container is a trace blood sample, and the second measurement mode is selected when the blood sample in the sample container is a normal blood sample; (2) when it is determined that the first measurement mode is selected, controlling the mixing device to drive the sample stirring component to move into the sample container to stir and mix the trace blood sample; (3) When it is determined that the second measurement mode is selected, the mixing device is controlled to invert the sample container to perform inversion mixing.

29. A method for analyzing a blood sample, include: The step of determining the measurement mode is as follows: determining whether the current measurement mode is the first measurement mode or the second measurement mode, wherein when the blood sample in the sample container is a trace blood sample, the first measurement mode is selected, and when the blood sample in the sample container is a normal blood sample, the second measurement mode is selected; The first test sample preparation step: when it is determined that the first measurement mode is selected, the first mixing device is controlled to drive the sample stirring component to mix the blood sample in the sample container, and the first sampling amount of the blood sample is pipetted to prepare the first test sample, wherein the sample container can hold a trace blood sample or a constant blood sample, and the sample stirring component can move up and down, and can move downward into the sample container to stir and mix the trace blood sample; A second test sample preparation step: when it is determined that the second measurement mode is selected, controlling the second mixing device to mix the blood sample in the sample container by inversion, and pipetting a second sampling amount of the blood sample to prepare a second test sample; and Testing step: testing the first testing sample or the second testing sample; The method further comprises: Injection mode determination step: determining whether the current injection mode is the first injection mode or the second injection mode; Sample rack transporting step: when it is determined that the first sample injection mode is selected, the sample transporting device is controlled to transport the sample rack containing the sample container to a predetermined position, and the mixed sample container is transported to the first sampling position; Sample chamber component closing step: when it is determined that the second sampling mode is selected, the sample chamber component is closed, and the sample container is sent to the second sampling position; In the first test sample preparation step, the second mixing device moves the sample container on the sample rack transported to the predetermined position by the sample transport device to the first mixing position for mixing; In the second test sample preparation step, the second mixing device grabs the sample rack or the sample container on the sample rack transported to a predetermined position by the sample transport device and mixes them by inversion.

30. The blood sample analysis method according to claim 29, Features: In the measurement mode determination step, it is also determined whether the current measurement mode is a third measurement mode; A third test sample preparation step: when it is determined that the third measurement mode is selected, controlling the first mixing device to mix the pre-diluted blood sample in the sample container, and pipetting a third sampling amount of the pre-diluted blood sample to prepare a third test sample; In the detection step, the third detection sample is detected.

31. The blood sample analysis method according to claim 29, It is characterized in that Also includes: When it is determined that the first measurement mode is selected, a first sampling amount of the mixed blood sample is pipetted from the first sample container to prepare a first test sample; When it is determined that the second measurement mode is selected, a second sample volume of the mixed blood sample is pipetted from the second sample container to prepare a second test sample; The first sampling amount is less than the second sampling amount.

32. The blood sample analysis method according to claim 31, It is characterized in that The first sampling volume is 5-50 μL.

33. The blood sample analysis method according to claim 32, It is characterized in that The first sampling volume is 15-35 μL.

34. The blood sample analysis method according to claim 29, It is characterized in that Also includes: Determine whether the current measurement mode is the third measurement mode; When it is determined that the third measurement mode is selected, a third sample amount of the mixed pre-diluted blood sample is pipetted from the first sample container to prepare a third detection sample.

35. The blood sample analysis method according to any one of claims 29 to 34, Features: The sample stirring component stirs the sample in one or a combination of rotation, circular orbit, linear swing or up and down oscillation.

36. The blood sample analysis method according to any one of claims 29 to 34, Features: The blood sample in the sample container mixed by the first mixing device is a whole blood sample, and the amount of the blood sample is 30-250 μL.

37. The blood sample analysis method according to claim 36, Features: The amount of the blood sample in the sample container is 50-200 μL.

38. The blood sample analysis method according to claim 36, Features: The amount of the blood sample in the sample container is 50-100 μL.

39. A computer storage medium storing computer executable instructions, which, when executed by at least one processor of a blood sample analyzer, cause the blood sample analyzer to perform the method according to any one of claims 29 to 38.

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