An enzyme reaction device with self-heating and water supply

Abstract

The present invention provides an enzyme reaction device with self-heating and water supply, comprising a reactor with a reaction hole on the upper surface, a condensation chamber in the reactor, a water accumulation groove on the upper surface of the condensation chamber, one end of the water accumulation groove is connected to the reaction hole, the water accumulation groove is inclined toward the reaction hole, a volatile cooling material is contained in the condensation chamber, a volatilization hole connected to the outside is provided on the wall of the condensation chamber, a heating paste is provided at the bottom of the reactor below the reaction hole and the condensation chamber, and an oxidative heating material is provided on the heating paste. The device can automatically provide the heat and water required for the enzyme reaction when in contact with the air, solving the problem that the water supply and temperature maintenance of the enzyme reaction device in a non-laboratory environment in the prior art need to rely on other equipment.

Description

Technical Field

[0001] The invention relates to the technical field of enzyme chemical equipment, in particular to an enzyme reaction equipment capable of self-heating and water supply. Background Art

[0002] Molecular biology is a science that studies the structure and function of biological macromolecules at the molecular level to clarify the phenomena and essence of life. The central content of its research is the information transmission and interaction of DNA, RNA and proteins, and enzymes play a key role in this. Enzymes are a type of protein produced by living cells that has a highly efficient catalytic effect on its specific substrate. Among them, the polymerase chain reaction technology derived from the application of enzymes is a molecular biology technology that relies on the enzymatic reaction of DNA polymerase to amplify specific DNA fragments in vitro. The polymerase chain reaction is achieved by temperature control. Under high temperature, double-stranded DNA fragments denature and dissociate to form two single-stranded DNA fragments; under low temperature, primers pair and bind with the complementary sequence of the template DNA single strand; under the action of DNA polymerase, with dNTP as the reaction raw material, the target sequence as the template, and the primer binding point as the starting point, according to the principle of base complementary pairing and semi-conservative replication, a new base fragment complementary to the template DNA chain is extended and synthesized.

[0003] Enzyme-catalyzed reaction is a chemical reaction catalyzed by enzymes. As a specific and efficient biological catalyst, enzymes can greatly increase the speed of chemical reactions. However, the catalytic effect of enzymes can only be maximized under specific temperature and humidity. Therefore, the supply of water and the guarantee of temperature in enzymatic reactions are very important.

[0004] In the prior art, in order to ensure the water supply and temperature maintenance in the enzymatic reaction in a non-laboratory environment, it is necessary to connect a third-party water supply device and a temperature control device to the reaction vessel, which has the problems of complex structure and high material cost. Summary of the invention

[0005] The present invention provides an enzyme reaction device with self-heating and water supply, which can automatically provide the heat and water required for the enzyme reaction when in contact with air, thereby solving the problem in the prior art that the water supply and temperature maintenance of the enzyme reaction device in a non-laboratory environment need to rely on other equipment.

[0006] To achieve the above-mentioned purpose, the present invention provides an enzyme reaction device with self-heating and water supply, including a reactor with reaction holes on the upper surface, and the special feature of the device is that a condensation chamber is provided in the reactor, a water accumulation groove is provided on the upper surface of the condensation chamber, one end of the water accumulation groove is connected with the reaction hole, and the water accumulation groove is inclined toward the reaction hole. Volatile cooling material is contained in the condensation chamber, and a volatilization hole connected to the outside is provided on the wall of the condensation chamber. A heating paste is provided at the bottom of the reactor below the reaction hole and the condensation chamber, and an oxidative heat-generating material is provided on the heating paste.

[0007] Any of the following schemes can be used as a further preferred scheme of the enzyme reaction device of the present invention:

[0008] The volatilization hole is connected to a volatilization capillary, the other end of the volatilization capillary is connected to an end of the water accumulation groove away from the reaction hole, and liquid sterile water is stored in the volatilization capillary.

[0009] The inner diameter of the volatile capillary is a capillary of less than 1 mm, and the volume of the liquid sterile water is less than 30 μL.

[0010] The oxidation heating material is reduced iron powder.

[0011] The volatile cooling material is liquid alcohol.

[0012] A rubber plug is arranged at the volatilization hole, and a through hole for the volatilization capillary to pass through is opened on the rubber plug.

[0013] The enzyme reaction device for self-heating and water supply is composed of N reactors arranged side by side, wherein N≥1.

[0014] The present invention provides an enzyme reaction device with self-heating and water supply. A heating paste is arranged at the bottom of a reaction hole and a condensation chamber. The oxidative heat-generating material on the heating paste contacts with air to generate an exothermic reaction, thereby heating the reaction hole, thereby solving the problem of heat supply for the enzyme reaction. At the same time, the volatile cooling material in the condensation chamber inside the reactor evaporates when heated, which can condense the water vapor above the water accumulation groove into water droplets, and can also push the liquid water in the volatilization capillary to move forward and be introduced into the reaction hole through the groove on the surface of the condenser, thereby solving the problem of water supply for the enzyme reaction.

[0015] The enzyme reaction device with self-heating and water supply of the present invention can effectively and automatically provide the water and heat required for the enzyme reaction. At the same time, the device has a simple structure, low cost and convenient application. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The schematic diagram is a structural diagram of an enzyme reaction device with self-heating and water supply.

[0017] Figure 2 for Figure 1 Schematic diagram of the structure of the rubber stopper.

[0018] Figure 3 The invention is an enzyme reaction device which is composed of multiple groups of reactors arranged side by side and provides self-heating and water supply. DETAILED DESCRIPTION

[0019] The following is a further detailed description of an enzyme reaction device for self-heating and water supply of the present invention in conjunction with the accompanying drawings and specific embodiments.

[0020] Example 1

[0021] As attached Figure 1 An enzyme reaction device with self-heating and water supply shown in the figure comprises a reactor 1, a reaction hole 3 is arranged on the upper surface of the reactor 1, a condensation chamber 2 is arranged inside the reactor 1, a water accumulation groove 4 is arranged on the upper surface of the condensation chamber 2, one end of the water accumulation groove 4 is connected with the reaction hole 3 and inclined toward the reaction hole 3, so as to facilitate the water flow into the reaction hole 3 and collect the substances to be detected (such as pathogens or harmful substances in the air). When liquid sterile water flows through the water accumulation groove, the substances to be detected can be flushed into the reaction hole 3. The condensation chamber 2 is filled with liquid alcohol, which is a volatile cooling material. The wall of the condensation chamber 2 is provided with a volatilization hole 5 connected to the outside world. A heating paste 6 is arranged at the bottom of the reactor 1 below the reaction hole 3 and the condensation chamber 2, and reducing iron powder is arranged on the heating paste 6.

[0022] The method of using the device is as follows: the device is sealed in a vacuum package after production to prevent loss of raw materials caused by reaction. When in use, the vacuum package is torn open, the device is taken out, and placed in the air. The heating sticker 6 contacts the air to produce an oxidation reaction and releases heat, thereby providing heat for the enzymatic reaction in the reaction hole 3; and heating the lower part of the condensation chamber 2 can begin to promote the volatilization of liquid alcohol. After the alcohol in the condensation chamber 2 is volatilized by heat, it is discharged through the volatilization hole 5. It begins to evaporate continuously and absorbs heat during the evaporation process, thereby reducing the surface temperature of the water accumulation groove 4, and then causing the water vapor in the air near its surface to condense into water droplets when it is cooled. The water droplets condensed inside and near the water accumulation groove 4 will flow into the reaction hole 3 along the inclined direction of the groove 4, thereby providing moisture for the enzymatic reaction.

[0023] Example 2

[0024] As attached Figure 1 , 23 shows an enzyme reaction device with self-heating and water supply, comprising a reactor 1, a reaction hole 3 is arranged on the upper surface of the reactor 1, a condensation chamber 2 is arranged inside the reactor 1, a water accumulation groove 4 is arranged on the upper surface of the condensation chamber 2, one end of the water accumulation groove 4 is connected with the reaction hole 3 and inclined toward the reaction hole 3, so as to facilitate the drainage into the reaction hole 3 and the collection of the substance to be detected (such as pathogens or harmful substances in the air). When liquid sterile water flows through the water accumulation groove, the substance to be detected can be flushed into the reaction hole 3. The condensation chamber 2 is filled with liquid The condensation chamber 2 has a volatilization hole 5 connected to the outside world on its wall, and a rubber plug 9 is clamped at the volatilization hole 5. The rubber plug 9 has a through hole 10 for the volatilization capillary 7 to pass through. The through hole 10 is connected to the volatilization capillary 7. The volatilization capillary 7 has an inner diameter of 1 mm and contains 25 microliters of liquid sterile water 8. Due to the capillary action principle, the liquid sterile water 8 will not flow out of the volatilization capillary 7 when not in use. The reaction hole 3 and the bottom of the condensation chamber 2 are both provided with a heating paste 6, and the heating paste 6 is provided with reducing iron powder. Nine reactors 1 are arranged side by side to form an enzyme reaction device with self-heating and water supply.

[0025] The method of using the device is as follows: the device is sealed in a vacuum package after production to prevent loss caused by raw material reaction. When in use, the vacuum package is torn open, the device is taken out, and placed in the air. The heating patch 6 contacts the air to generate an oxidation reaction and generate heat, thereby providing heat for the enzymatic reaction in the reaction hole 3; heating the lower part of the condensation chamber 2 can begin to promote the volatilization of liquid alcohol. After the alcohol in the condensation chamber 2 is volatilized by heat, its volume expands, pushing the liquid sterile water 8 stored in the volatilization capillary 7 to be discharged from the volatilization capillary 7 into the water accumulation groove 4, and then flowing downward into the reaction hole 3, providing water for the enzymatic reaction in the reaction hole 3. At the same time, the alcohol in the condensation chamber 2 evaporates and absorbs heat, thereby reducing the temperature of the water accumulation groove 4, and then causing the water vapor in the air near its surface to condense into water droplets when it is cooled and flow into the reaction hole 3 along the inclined direction of the water accumulation groove 4, thereby continuing to provide water for the enzymatic reaction.

[0026] In the enzyme reaction device of the self-heating and water supply of the present embodiment 1 and embodiment 2, the reduced iron powder in the heating paste 6 oxidizes and generates heat after contacting with the air. According to the change of the heating temperature, it can be divided into three stages: a slow heating stage, a rapid heating stage, and a slow cooling stage. When the heating paste pasted on the back of the condenser shell begins to contact with the air, the temperature rises slowly, and after a period of time, the temperature may rise sharply. When the temperature reaches the flash point of the alcohol solvent volatilization and above the flash point, the alcohol solvent in the cavity of the condenser shell evaporates, pushing the liquid sterile water in the volatilization capillary to move forward, flowing through the groove and flowing into the reaction hole. When the temperature reaches the optimum temperature range of the enzyme reaction (55-65°C), the enzyme system begins to react with the inflow of liquid sterile water and the heating effect of the heating paste on the back of the shell.

Claims

1. An enzyme reaction device for self-heating and water supply, comprising a reactor (1) having reaction holes (3) arranged on the upper surface, characterized in that: The reactor (1) is provided with a condensation chamber (2), the upper surface of the condensation chamber (2) is provided with a water accumulation groove (4), one end of the water accumulation groove (4) is connected to the reaction hole (3), the condensation chamber (2) is filled with volatile cooling material, the wall of the condensation chamber (2) is provided with a volatilization hole (5) connected to the outside, and the bottom of the reactor (1) is provided with a heating paste (6) below the reaction hole (3) and the condensation chamber (2), and the heating paste (6) is provided with an oxidative heat-generating material.

2. The enzyme reaction device for self-heating and water supply according to claim 1, characterized in that: The volatilization hole (5) is connected to a volatilization capillary (7), the other end of the volatilization capillary (7) is connected to an end of the water accumulation groove (4) away from the reaction hole (3), and liquid sterile water (8) is stored inside the volatilization capillary (7).

3. The enzyme reaction device for self-heating and water supply according to claim 2, characterized in that: The volatile capillary (7) has an inner diameter of less than 1 mm, and the volume of the liquid sterile water (8) is less than 30 μl.

4. The enzyme reaction device for self-heating and water supply according to claim 1, characterized in that: The oxidation heating material is reduced iron powder.

5. The enzyme reaction device for self-heating and water supply according to claim 1, characterized in that: The volatile cooling material is liquid alcohol.

6. The enzyme reaction device for self-heating and water supply according to claim 2, characterized in that: A rubber plug (9) is provided at the volatilization hole (5), and a through hole (10) is provided on the rubber plug (9) for the volatilization capillary (7) to pass through.

7. The enzyme reaction device for self-heating and water supply according to any one of claims 1 to 6, characterized in that: It is composed of N reactors (1) arranged side by side, where N≥1.

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