Method of Tissue Culture and Rapid Propagation of Weeping Flowers

Abstract

The invention discloses a method for tissue culture and rapid propagation of weeping tube flowers, which comprises the following steps: setting a culture medium; selecting and disinfecting explants to ensure that there are no defects in the outer scales; putting the scale buds after the disinfection treatment on the induction medium Carry out the induction culture of adventitious buds; the induced adventitious bud clusters are multiplied by adventitious buds, and the multiplied adventitious bud clusters are divided into individual plants under aseptic conditions and then transferred to the rooting medium for rooting induction culture; After the induction of rooting, the weeping tube flower plants are transplanted and hardened. The invention can obtain a large amount of high-quality and strong seedlings of the weeping tube flower with the same genetic character in a short period of time.

Description

technical field

[0001] The invention relates to a method for tissue culture and rapid propagation of weeping tube flowers. Background technique

[0002] Cyrtanthus spp. is a perennial bulbous plant of the genus Cyrtanthus in the family Amaryllidaceae, also known as Cyrtanthus spp. It blooms in winter and spring. It has a unique flower shape, a long flowering period, and evergreen leaves. It is an excellent potted ornamental flower. Its flowers have a sweet fragrance, and the flower stems are slender and elegant and upright. It can also be used as a high-grade cut flower material.

[0003] Since the pendant flower is native to Africa, its market price is relatively high, especially some rare varieties are even more expensive. Weeping tube flowers mainly rely on ramet propagation, but the reproductive efficiency is low, the cycle is long and limited by the amount of female parent material, it is difficult to meet the needs of the market. Theoretically, using plant tissue cu...

Examples

Embodiment 1

[0085] Embodiment 1, a kind of method for tissue culture rapid propagation of vertical tube flower:

[0086] Step 1, the preparation of culture medium:

[0087] Induction medium: MS+6-BA 1.0mg / L+NAA 0.01mg / L;

[0088] Proliferation medium: MS+6-BA 0.5mg / L+NAA 0.02mg / L;

[0089] Rooting medium: 1 / 2MS+NAA 0.1mg / L+IBA 0.1mg / L;

[0090] All the other are the same as "method 1, a kind of tissue culture and rapid propagation method of weeping tube flower", and the obtained results are as follows:

[0091] Step 3, induction of adventitious buds: after about 3 weeks, adventitious bud clusters can be induced; the induction rate of adventitious buds is 100%;

[0092] Step 4, proliferation and subculture of adventitious buds: the proliferation coefficient was 3.17 when cultured for 4 weeks. Subculture every 4 weeks.

[0093] Step 5, rooting induction: the rooting culture time is 8 days; at this time, the root primordia are induced to form.

[0094] Step 6, harden the seedlings for ...

Embodiment 2-7

[0095] Embodiment 2-7, a method for tissue culture and rapid propagation of T. chinensis, the difference from Example 1 is as described in Table 1 below.

[0096] Table 1

[0097]

Embodiment 8-13

[0098] Embodiment 8-13, a kind of tissue culture rapid propagation method of weeping tube flower, and the difference of embodiment 1 is described in the following table 2.

[0099] Table 2

[0100]

[0101]

technical field

[0001] The invention relates to a method for tissue culture and rapid propagation of weeping tube flowers. Background technique

[0002] Cyrtanthus spp. is a perennial bulbous plant of the genus Cyrtanthus in the family Amaryllidaceae, also known as Cyrtanthus spp. It blooms in winter and spring. It has a unique flower shape, a long flowering period, and evergreen leaves. It is an excellent potted ornamental flower. Its flowers have a sweet fragrance, and the flower stems are slender and elegant and upright. It can also be used as a high-grade cut flower material.

[0003] Since the pendant flower is native to Africa, its market price is relatively high, especially some rare varieties are even more expensive. Weeping tube flowers mainly rely on ramet propagation, but the reproductive efficiency is low, the cycle is long and limited by the amount of female parent material, it is difficult to meet the needs of the market. Theoretically, using plant tissue cu...

Examples

Embodiment 1

[0085] Embodiment 1, a kind of method for tissue culture rapid propagation of vertical tube flower:

[0086] Step 1, the preparation of culture medium:

[0087] Induction medium: MS+6-BA 1.0mg / L+NAA 0.01mg / L;

[0088] Proliferation medium: MS+6-BA 0.5mg / L+NAA 0.02mg / L;

[0089] Rooting medium: 1 / 2MS+NAA 0.1mg / L+IBA 0.1mg / L;

[0090] All the other are the same as "method 1, a kind of tissue culture and rapid propagation method of weeping tube flower", and the obtained results are as follows:

[0091] Step 3, induction of adventitious buds: after about 3 weeks, adventitious bud clusters can be induced; the induction rate of adventitious buds is 100%;

[0092] Step 4, proliferation and subculture of adventitious buds: the proliferation coefficient was 3.17 when cultured for 4 weeks. Subculture every 4 weeks.

[0093] Step 5, rooting induction: the rooting culture time is 8 days; at this time, the root primordia are induced to form.

[0094] Step 6, harden the seedlings for ...

Embodiment 2-7

[0095] Embodiment 2-7, a method for tissue culture and rapid propagation of T. chinensis, the difference from Example 1 is as described in Table 1 below.

[0096] Table 1

[0097]

Embodiment 8-13

[0098] Embodiment 8-13, a kind of tissue culture rapid propagation method of weeping tube flower, and the difference of embodiment 1 is described in the following table 2.

[0099] Table 2

[0100]

[0101]

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