A kind of method for plant regeneration of camphor variety Yongjin

The technology of a variety and camphor, applied in the field of plant regeneration of the camphor variety Yongjin, can solve the problems of black death, new leaves falling off, and difficult rooting of clustered buds, achieving strong growth potential, consistent genetic traits, overcoming axillary bud induction and difficulty. The effect of growing difficulties

CN103782906BInactive Publication Date: 2016-03-30NINGBO CITY COLLEGE OF VOCATIONAL TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2016-03-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for regenerating a camphor variety yongjin (Chinese character) plant. The method comprises the following steps: (1) collecting a new twig of camphor variety 'yongjin', which grows in current year, removing leaves after the new twig is taken back to a laboratory; cutting into stems with 1-2 auxiliary buds, asepticizing the stems, then inoculating stems into MS culture medium containing 6-benzyl amino adenine and indolebutyric acid to induce formation of the auxiliary buds; (2) inoculating the 'yongjin' auxiliary buds which grow well into the MS culture medium containing 6-benzyl amino adenine and indolebutyric acid together with the stems, so as to carry out subculture and multiplication cultivation; (3) cutting proliferated cluster buds which are small, and grow well, with the mean length being about 2.0cm by using a dissecting knife, and then inoculating the cut cluster buds into 1 / 2 MS culture medium containing indolebutyric acid so as to induce formation of adventitious roots. By adopting the method disclosed by the invention, the phenomena of difficult auxiliary buds induction and growth, black death of subcultured and proliferated new leaves, dropping, and weak growth are overcome, and an asepsis material is sub-cultured for a long period of time. Thus, great proliferation is achieved, the induced cluster buds are vigorous in growth, and the inheritable characters of the induced cluster buds are same as those of a female parent.
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Description

technical field

[0001] The invention belongs to the field of tissue culture, and in particular relates to a method for plant regeneration of the camphor variety Yongjin. Background technique

[0002] Camphor tree (Cinnamomumcaphora), also known as camphor, is a subtropical evergreen broad-leaved tree of the genus Lauraceae. It grows rapidly, has luxuriant branches and leaves, is evergreen in all seasons, and has beautiful leaf shape and strong fragrance. It is not only a precious aromatic oil tree species but also an important landscape and greening tree species in cities. Cinnamomum camphora new variety Yongjin (Cinnamomumcamphora'Yongjin', National Plant Variety Right No. 2009009) is a variant of camphor seed, with bright red branches, new leaves and flowers in golden yellow, yellow peel, and branches, leaves, The color of the peel has seasonal changes. As a tall tree with colorful leaves, it has very high potential and value for garden viewing and promotion and applicati...

Examples

Embodiment 1

[0023] The experimental material of the present invention is taken from the new camphor variety Yongjin plant grown for 14 years at the Qiuai Base of the Seedling Center of Ningbo Forestry Bureau. Take well-developed and semi-lignified side shoots and top shoots, remove leaves, cut into stems with 1-2 axillary buds, wash with detergent for 4-5 minutes, and rinse with running water for more than 30 minutes. Disinfect with 75% alcohol for 3 minutes on the ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 0.1% mercury chloride according to the diameter of the stem segment for 8-12 minutes, and rinse with sterile water for 4-5 times. Blot the water with filter paper, cut off the browned part of the stem, and inoculate it into hormone-free MS medium. Select aseptic stems as explants of axillary buds and inoculate them into the induction medium of MS+6-BA1.0mg / L+IBA0.02mg / L. After 20 days, the stems of axillary buds begin to germinate and continue to g...