Chrysanthemum one-step tissue culture quick breeding method

A technology for tissue culture and rapid propagation of chrysanthemums, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as increasing difficulty and complicated operation steps, and achieve the goal of reducing operation difficulty, improving cultivation efficiency, and low production cost Effect

CN102939901BActive Publication Date: 2013-08-14SICHUAN COLORLINK CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2013-08-14

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Abstract

The invention discloses a chrysanthemum one-step tissue culture quick breeding method. The method comprises the following steps of S1, obtaining aseptic tissue culture seedlings, collecting chrysanthemum explants, cutting the chrysanthemum explants into sections with buds and stems, sterilizing, cleaning, inoculating the sections to a culture medium to grow, transferring the sections to a new culture medium to grow after the new buds grow to be 2 to 3cm, and obtaining the aseptic robust maternal seedling with roots; and S2, one-step quick breeding, cutting the aseptic robust maternal seedlingobtained in S1 into sections with buds and stems, inoculating the sections to the culture medium to carry out passage and rooting culture, and enabling the sections with buds and stems to grow into complete plants. The method has the beneficial effects that the chrysanthemum tissue culture seedlings with roots do not need special seedling training, the robust growth is realized, the production cost is low, the genetic property of the obtained tissue culture seedling is uniform, the breeding coefficient is high, the breeding period is short, and the method is an effective path for the chrysanthemum tissue culture quick breeding; the culture medium of the tissue culture and the culture medium of the robust seedlings and roots belong to the same type of culture medium, so the operation difficulty is reduced, the culture efficiency is improved, and the application range is wide.
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Description

Technical field

[0001] The present invention involves the technical field of chrysanthemum cultivation methods, especially the one -step method of the chrysanthemum. Background technique

[0002] Chrysanthemum, perennial chrysanthemum herbaceous plant, is one of the top ten famous flowers in China, has a long history of cultivation and selection.Chrysanthemum is also a commonly used Chinese medicine in China.It mainly treats headache, dizziness, red eyes, heart chest annoyance, sore treatment, swelling and other symptoms.Modern pharmacological studies have shown that chrysanthemums have a variety of pharmacological activity such as treating coronary heart disease, reducing blood pressure, preventing hyperlipidemia, antibacterial, antidoxin, anti -inflammatory, and anti -aging.

[0003] There are generally two breeding methods of chrysanthemum breeding, namely nutritional reproduction and seed reproduction.In order to maintain the excellent characteristics of chrysanthemums, nutri...

Examples

Embodiment 1

[0017] The chrysanthemum one -step method of cultivation method, it includes the following steps:

[0018] S1. Get the seedlings of chrysanthemum sterile group: collect chrysanthemum outer implants, cut into 2 to 3cm bud stalk sections, sterilizes of alcohol sterilization 30, sterilized sterilization of sterilization of 5 minutes, sterilized water for 5 times, vaccinated in the medium base basisGrowing, such as figure 1 It is shown that the medium is MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the cultivation conditions of 2000lx, 16 h, and temperature 24 ° C; a week later, the new axillary buds germinate to 2 to 2 ~When 3cm, the new medium is used to grow. The medium is still MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the training conditions of 2000LX, 16 h, and temperature 24 ° C.Two weeks after growing, get rooted in a sterile and strong mother, such as figure 2 Show;

[0019] S2, one step fast complication: cut the sterile and strong mother's seedlings obtained in s...

Embodiment 2

[0021] The chrysanthemum one -step method of cultivation method, it includes the following steps:

[0022] S1. Get the seedlings of chrysanthemum sterile group: collect chrysanthemum outer implants, cut into 2 to 3cm bud stalk sections, sterilizes of alcohol sterilization 20s, sterilized sterilization of mercury, washed 4 times with sterile water, vaccinated in the medium base basisGrowing, such as figure 1 It is shown that the medium is MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the cultivation conditions of 2000lx, 16 h, and temperature 24 ° C; a week later, the new axillary buds germinate to 2 to 2 ~When 3cm, the new medium is used to grow. The medium is still MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the training conditions of 2000LX, 16 h, and temperature 24 ° C.Two weeks after growing, get rooted in a sterile and strong mother, such as figure 2 Show;

[0023] S2, one step fast complication: cut the sterile and strong mother's seedlings obtained in step S1 into 2...

Embodiment 3

[0025] The chrysanthemum one -step method of cultivation method, it includes the following steps:

[0026] S1. Get the seedlings of chrysanthemum sterile group: collect chrysanthemum outer implants, cut into 2 to 3cm bud stalk sections, sterilizes 40s of alcohol, sterilizes for mercury, and sterilizes 4 min, so washed 4 times, vaccinated in the medium baseGrowing, such as figure 1 It is shown that the medium is MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the cultivation conditions of 2000lx, 16 h, and temperature 24 ° C; a week later, the new axillary buds germinate to 2 to 2 ~When 3cm, the new medium is used to grow. The medium is still MS+NAA0.2mg / L+IAA0.1mg / L. It is cultivated under the training conditions of 2000LX, 16 h, and temperature 24 ° C.Two weeks after growing, get rooted in a sterile and strong mother, such as figure 2 Show;

[0027] S2, one step fast complication: cut the sterile and strong mother's seedlings obtained in step S1 into 20-30 segments, 2 to 3cm bud...