A device for non-toxic labeling of in vitro culture dishes in cell therapy
By designing a non-toxic marking device containing cold laser engraving technology and ultraviolet disinfection lamps, the problems of chemical marker pollution and work burden were solved, and the non-toxic, fast and accurate marking of cell culture dishes was achieved, ensuring the experimental Cleanliness and efficiency.
Patent Information
- Application Number
- CN202110291007.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-03-18
- Publication Date
- 2024-12-17
- Estimated Expiration
- 2041-03-18
AI Technical Summary
The use of chemical markers in the prior art has problems of toxic contamination on cell culture dish marking and increasing the work burden of doctors, especially in cell therapy, which has a greater impact on sensitive cell types.
A non-toxic marking device including an upper shell, a Petri dish platform, a lower chassis and a marking head was designed. It uses cold laser cavitation technology and multiple exhaust devices, combined with ultraviolet disinfection lamps to ensure the cleanliness of the marking process. and efficiency.
A non-toxic, fast and accurate cell culture dish labeling is achieved, which reduces the work burden of doctors, improves work efficiency, and prevents bacterial infection through ultraviolet disinfection lamps to ensure the accuracy of experimental results.
Smart Images
Figure CN112872608B_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of medical devices, in particular to a device for non-toxic labeling of in vitro culture dishes in cell therapy. Background Art
[0002] Currently, cell culture dishes are manually marked using chemical markers. Chemical pigments have obvious organic toxic properties. In a closed incubator, this toxic substance will continue to spread and pollute the culture environment. For more sensitive cell types (especially stem cells and human embryos), the trace accumulation and continuous existence of this organic toxicant has the potential risk of teratogenicity, carcinogenicity, genotoxicity, and epigenetic toxicity. In recent years, with the gradual development of cell therapy in the clinic, changes in stem cell phenotypes and genetic characteristics caused by operational factors have caused concerns among scientists and clinicians. Therefore, it is urgent to develop a non-toxic and easy-to-identify method for important quality control in the process of in vitro cell therapy.
[0003] Chinese patent application: CN200420013683.6 discloses a cell culture dish with positioning marks, including a symmetrical upper cover mounted on a disc with a cylindrical wall, and a positioning coordinate grid is set on the back of the bottom of the disc. When using the present invention to culture cells or conduct cell cloning experiments in a laboratory, according to the position of the cultured cells or cloned cells on the positioning coordinate grid on the bottom of the disc, it is convenient and accurate to perform positioning operations or record, manage and communicate on single cells or cloned cells. The drawbacks of inconvenience in operation, recording and communication caused by different personal positioning habits of the experimenter are avoided. However, the device is mainly used to mark the culture dish manually, which unnecessarily increases the workload of the doctor and reduces the efficiency of the work. At the same time, in the process of marking, it is easy to cause bacterial infection due to being placed outside, affecting the experimental effect, thereby causing inaccurate information, so it is not conducive to promotion and use.
[0004] In summary, there is an urgent need for a device for non-toxic labeling of in vitro culture dishes in cell therapy, which has a simple structure, is easy to operate, can reduce the workload of doctors, improve work efficiency, and facilitate rapid labeling of culture dishes. However, there is currently no report on this new type of device for non-toxic labeling of in vitro culture dishes in cell therapy. Summary of the invention
[0005] The purpose of the present invention is to mark the identity, operation and other related information of the cell culture dish when culturing cells in vitro during clinical cell therapy. It specifically provides a device for non-toxic marking of in vitro culture dishes in cell therapy.
[0006] To achieve the above object, the technical solution adopted by the present invention is:
[0007] A device for non-toxic marking of in vitro culture dishes in cell therapy, comprising an upper shell, a culture dish platform, a lower chassis and a marking head located inside the upper shell, wherein a wiring column is arranged on the outer side of the upper shell, and the wiring column is in a U-shaped structure, one end of which is connected to the marking head inside the upper shell, and the other end is connected to the lower chassis below the upper shell; air inlets for the marking area are arranged on both sides of the marking head, and an air outlet for the marking area is also arranged on the bottom surface of the marking head; a laser head is also connected below the marking head, and a plurality of laser emission ports are arranged on the laser head; the upper shell also comprises a top cover, which is a snap-on top cover, and the top cover is rotatably connected to the upper shell, and a detachable disinfection lamp mounting plate is also arranged on the inner surface of the top cover, and a plurality of purple External disinfection lamp; the bottom end of the upper shell is seamlessly fixedly connected to the culture dish platform, the upper surface of the culture dish platform is provided with a groove, a culture dish placement plate is provided in the groove, and the culture dish placement plate is detachably connected to the culture dish platform, and a handle is also fixedly provided on the outer surface of the culture dish placement plate; the lower part of the culture dish platform is fixedly connected to a lower chassis, a motor is provided inside the lower chassis, the motor is electrically connected to the wiring column and the marking head, a biochemical filter material replacement port is provided opposite to the lower chassis, an opening and closing plate is also provided at the biochemical filter material replacement port, a switch button is provided above the biochemical filter material replacement port, the switch button is electrically connected to the motor, and chassis heat dissipation holes are also provided on both sides of the lower chassis; a supporting anti-slip foot pad is also provided at the bottom of the lower chassis.
[0008] In the above-mentioned device for non-toxic marking of in vitro culture dishes in cell therapy, as a preferred embodiment, the upper shell is made of transparent acrylic material, and a layer of ultraviolet protection film is also provided on its outer surface.
[0009] In the above-mentioned device for non-toxic labeling of in vitro culture dishes in cell therapy, as a preferred embodiment, the culture dish placement plate is provided with a plurality of culture dish placement slots of different specifications and shapes.
[0010] In the above-mentioned device for non-toxic marking of in vitro culture dishes in cell therapy, as a preferred embodiment, the marking head uses cold laser burning technology for marking.
[0011] In the above-mentioned device for non-toxic labeling of in vitro culture dishes in cell therapy, as a preferred embodiment, a gap of 0.4-1.2 cm is left between the culture dish platform and the lower chassis, and the gap serves as a discharge port for the culture dish area.
[0012] In the above-mentioned device for non-toxic labeling of in vitro culture dishes in cell therapy, as a preferred embodiment, a USB external interface and a power connection port are also provided on the back of the lower chassis.
[0013] The present invention has the advantages that:
[0014] 1. The present invention is simple to operate, easy to use, and highly practical. It can quickly and accurately mark culture dishes, improve work efficiency, and reduce the workload of doctors. It is also small in size and can be conveniently placed in the laminar flow operation box of the cell culture room.
[0015] 2. The cold laser engraving technology adopted in the present invention has the advantages of low energy, precise control of engraving depth and width, and high speed. In addition, the energy is low and the amount of smoke generated is small, which will not affect the culture dish in the shell. The arrangement of multiple exhaust devices and air intake devices in the device can ensure the cleanliness of the environment in the marking chamber, avoid unnecessary infection of the culture dish by bacteria, and affect the experimental results.
[0016] 3. The culture dish placement slot described in the present invention can be set to different sizes and shapes as needed to meet the needs of use in different situations and increase the practicality of the device; and the transparent acrylic material used in the upper shell can facilitate workers to observe the internal marking situation, and the ultraviolet protection film located on the surface of the shell can avoid the glare of strong light during marking / red light identification while observing, thereby effectively protecting the eyesight of the operator. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Attached Figure 1 It is a schematic diagram of the structure of the device for non-toxic labeling of in vitro culture dishes in cell therapy described in the present invention.
[0018] Attached Figure 2 It is a partial structural schematic diagram between the marking head and the lower case of the device for non-toxic marking of in vitro culture dishes in cell therapy described in the present invention.
[0019] Attached Figure 3 It is a partial structural schematic diagram of a marking head, a top cover and a culture dish platform on the device for non-toxic marking of in vitro culture dishes in cell therapy described in the present invention.
[0020] Attached Figure 4 It is a partial structural schematic diagram of the upper and lower chassis of the device for non-toxic labeling of in vitro culture dishes in cell therapy described in the present invention. DETAILED DESCRIPTION
[0021] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the appended claims of the application equally.
[0022] The reference numerals and components involved in the drawings are as follows:
[0023] 1. Upper housing 2. Petri dish platform 3. Lower housing
[0024] 4. Marking head 5. Wiring column 6. Air inlet of marking area
[0025] 7. Marking area exhaust 8. Laser head 9. Laser emission port
[0026] 10. Top cover 11. Disinfection lamp installation plate 12. Ultraviolet disinfection lamp
[0027] 13. Groove 14. Petri dish placement plate 15. Handle
[0028] 16. Biochemical filter material replacement port 17. Switch button 18. Support non-slip foot pad
[0029] 19. Ultraviolet protection film 20. Culture dish placement slot 21. USB external interface
[0030] 22. Power connection port 23. Opening and closing plate 24. Chassis cooling hole
[0031] 25. Motor
[0032] Example 1 A device for non-toxic labeling of in vitro culture dishes in cell therapy
[0033] Please see attached Figure 1-4 As shown, attached Figure 1 Schematic diagram of the structure of the device for non-toxic labeling of in vitro culture dishes in cell therapy described in the present invention. Figure 2 This is a partial structural schematic diagram of the upper marking head and the lower chassis of the device for non-toxic marking of in vitro culture dishes in cell therapy described in the present invention. Figure 3 This is a partial structural schematic diagram of the marking head, top cover and culture dish platform on the device for non-toxic marking of in vitro culture dishes in cell therapy described in the present invention. Figure 4 It is a partial structural schematic diagram of the upper and lower chassis of the device for non-toxic labeling of in vitro culture dishes in cell therapy described in the present invention.
[0034] The present invention mainly solves the problem that when a chemical marker is used to mark a cell culture dish manually, the chemical substance used is toxic, pollutes the culture environment, and the manual marking increases the workload of doctors unnecessarily. In this regard, a mechanized device is provided which is non-toxic, convenient for identity marking, and can reduce the workload of doctors. The device is specifically a device for non-toxic marking of in vitro culture dishes in cell therapy. The device mainly includes an upper shell 1, a culture dish platform 2, a lower chassis 3, and a marking head 4 located inside the upper shell. A wiring column 5 is arranged on the outer side of the upper shell 1, and the wiring column 5 is in a U-shaped structure, one end of which is connected to the upper shell. The marking head 4 inside the body is connected, and the other end is connected to the lower chassis 3 under the upper shell. The wiring column 5 is provided with a wire connecting the marking head and the motor in the lower chassis. The wire is used to control the marking operation of the marking head, and the wiring column 5 can also be used as a handrail to take the device, which is convenient for the operator to use clinically; the marking head 4 is provided with a marking area air inlet 6 on both sides, and the bottom of the marking head is also provided with a marking area exhaust 7, and the marking area air inlet 6 and the marking area exhaust 7 can cooperate with the fan in the motor in the lower chassis, so as to maintain the exhaust / exhaust during the process of meeting the standard, and ensure The environment in the marking chamber; a laser head 8 is also connected to the bottom of the marking head 4, and a plurality of laser emission ports 9 are arranged on the laser head 8. The laser head marks the identity of the culture dish on the culture dish platform through the laser emission ports. The operation is fast, simple and convenient, which improves the work efficiency of manual marking, saves time, and reduces the workload of doctors; the upper shell also includes a top cover 10, which is a snap-on top cover, and the top cover 10 is rotatably connected to the upper shell 1. During the marking process of the device, if a marking error occurs, the top cover can be opened for secondary focusing and adjustment of the platform height. , cleaning of the laser emission port, etc., to ensure the accuracy of marking; a detachable disinfection lamp mounting plate 11 is also provided on the inner surface of the top cover 10, and a plurality of ultraviolet disinfection lamps 12 are provided on the disinfection lamp mounting plate 11. The ultraviolet disinfection lamp 12 can sterilize and disinfect the bacteria generated in the upper shell, thereby avoiding bacterial infection of the culture dish during the marking process, affecting the experimental effect, and also ensuring the sterility of the culture dish. The setting of the detachable disinfection lamp mounting plate can facilitate the timely replacement of the ultraviolet disinfection lamp, thereby avoiding affecting the sterilization effect of the culture dish in the shell during marking, thereby affecting the marking process;The bottom end of the upper shell 1 is seamlessly fixedly connected to the culture dish platform 2, and a groove 13 is provided on the upper surface of the culture dish platform 2. A culture dish placement plate 14 is provided in the groove 13, and the culture dish placement plate 14 is detachably connected to the culture dish platform 2, and a handle 15 is fixedly provided on the outer surface of the culture dish placement plate, and the handle 15 can be convenient for taking out the culture dish placement plate in the groove, and the detachable design of the culture dish placement plate 14 and the culture dish platform 2 can facilitate the replacement of culture dish placement plates 14 with different specifications and shapes, thereby improving the practicality of the device; a lower chassis 3 is fixedly connected to the bottom of the culture dish platform 2, and a motor 25 is provided inside the lower chassis 3, and the motor 25 is electrically connected to the wiring column 5 and the marking head 4, and a biochemical filter material replacement port 16 is provided directly opposite to the lower chassis 3, and an opening and closing plate 23 is also provided at the biochemical filter material replacement port. The biochemical filter element replacement port is used to replace the filter element in the lower body (which can refer to the air filter element on the car). A switch button 17 is arranged above the biochemical filter material replacement port 16. The switch button 17 is electrically connected to the motor 25. The two sides of the lower chassis 3 are also provided with chassis heat dissipation holes 24. When the switch button is turned on, the motor starts to work. The chassis heat dissipation holes 24 located on both sides of the lower chassis timely discharge the heat generated by the motor, avoiding motor failure caused by the failure to discharge the heat in time due to long-term operation of the motor, affecting the marking process; the bottom of the lower chassis 3 is also provided with a supporting non-slip foot pad 18, which can be composed of a support rod and a non-slip rubber plug connected to the bottom end of the support rod, so as to support the device, and also prevent the device from sliding sideways to cause cross infection between culture dishes, avoiding unnecessary work burden. ;
[0035] In this embodiment, the upper shell 1 is preferably made of transparent acrylic material, and a layer of ultraviolet protection film 19 is also provided on its outer surface. The transparent acrylic material can facilitate workers to observe the internal marking situation, and the ultraviolet protection film 19 provided on the surface of the upper shell can avoid the glare of strong light during marking / red light marking while observing, thereby effectively protecting the eyesight of the operator.
[0036] In this embodiment, it is preferred that the culture dish placement plate 14 is provided with a plurality of culture dish placement slots 20 of different specifications and shapes. The culture dish placement slots 20 can be set to different sizes and shapes as needed to meet the use in different situations and increase the practicality of the device. At the same time, the culture dish placement slots set on the culture dish placement plate can fix the culture dish in the slot, preventing the culture dish from being offset due to external forces during marking, and thus making it possible to always keep the marking surface of the culture dish in the effective marking area (marking surface) without focusing the laser emission port, thereby facilitating the marking of the culture dish, improving work efficiency and the number of markings, and reducing the workload of the operator.
[0037] In this embodiment, the marking head 4 preferably adopts cold laser burning technology for marking, and the cold laser burning technology has the advantages of low energy, precise control of burning depth and width, and high speed. In addition, due to the low energy, the amount of smoke generated is small, which can avoid excessive smoke affecting the marking effect.
[0038] In this embodiment, it is preferred that a gap of 0.4-1.2 cm is left between the culture dish platform 2 and the lower chassis 3, and the gap serves as a culture dish area discharge port, which can discharge an appropriate amount of smoke generated during the marking process, and can also indirectly discharge gas smoke to the marking area exhaust port on the marking head, thereby avoiding that the smoke in the upper shell is too large and cannot be discharged in time, affecting the marking effect.
[0039] In this embodiment, a USB external interface 21 and a power connection port 22 are preferably provided on the back of the lower chassis 3. The USB external interface 21 can be connected to the internal system of the hospital, and the marked information can be uploaded to the internal system of the hospital to match the patient's identity information, so as to facilitate the subsequent filing and storage of the patient's medical information. At the same time, the USB external interface 21 can also be connected to external devices such as computer monitors and other control panels, so as to facilitate the control of the marking operation and observe the real-time status of the marking, and has the characteristics of simplicity and intuitiveness.
[0040] The method of using the present invention is as follows: first, the device is placed in a flat position, and the supporting non-slip foot pads at the bottom of the lower chassis can prevent the device from sliding; then, the USB external interface and the power connection block on the rear side are connected, and then the culture dish prepared in advance is placed in the placement groove of the culture dish placement plate, and then the culture dish placement plate with the culture dish is placed into the groove of the culture dish platform, and then the top cover is opened, and the marking head is accurately adjusted to match the marking interval position; then the top cover is closed and fixed; then the switch button is turned on to control the function button on the external display to perform real-time operation and recording during the marking process; after the marking is completed, the culture dish placement plate can be taken out.
[0041] It should be noted that the present invention is simple to operate, easy to use, and highly practical. It can quickly and accurately mark the culture dish, improve the work efficiency, reduce the workload of the doctor, and has a small size, which can be conveniently placed in the laminar flow operation box of the cell culture room; at the same time, the cold laser burning technology adopted by the device has the advantages of low energy, precise control of the burning depth and width, and fast speed, and low energy, small amount of smoke generated, and will not affect the culture dish in the shell; and the setting of multiple exhaust devices and air intake devices in the device can ensure the cleanliness of the environment in the marking chamber, and avoid unnecessary infection of the culture dish by bacteria, affecting the experimental results; the culture dish and the culture dish placement plate used in the device are made of polystyrene material, which is non-toxic, non-teratogenic, non-genotoxic, and non-carcinogenic, and can be set according to different specifications and models according to needs to meet clinical needs; and the protective shell of the printing area in the device is made of transparent acrylic board material, and in order to prevent the glare of strong light during marking / red light identification, the ultraviolet protection film (dark film) located on the outer layer can effectively protect the eyesight of the operator.
[0042] The above is only a preferred embodiment of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and supplements without departing from the principle of the present invention. These improvements and supplements should also be regarded as the scope of protection of the present invention.
Claims
1. A device for non-toxic labeling of in vitro culture dishes in cell therapy, characterized in that: It includes an upper shell, a petri dish platform, a lower chassis, and a marking head located inside the upper shell. There are wire routing columns arranged on the outer side of the upper shell, and the wire routing columns are in a U-shaped structure. One end is connected to the marking head inside the upper shell, and the other end is connected to the lower chassis below the upper shell; on both side surfaces of the marking head, there are air inlets for the marking area, and on the bottom surface of the marking head, there is also an air outlet for the marking area. Below the marking head, a laser head is further connected, and multiple laser emission ports are arranged on the laser head; the upper shell further includes a top cover, the top cover is a snap-on top cover, and the top cover is rotatably connected to the upper shell. A detachable disinfection lamp mounting plate is also arranged on the inner surface of the top cover, and several ultraviolet disinfection lamps are arranged on the disinfection lamp mounting plate; the bottom end of the upper shell is fixedly connected to the petri dish platform seamlessly. There are grooves arranged on the upper surface of the petri dish platform, and a petri dish placement plate is arranged in the grooves. The petri dish placement plate is detachably connected to the petri dish platform, and a handle is fixedly arranged on the outer surface of the petri dish placement plate; a lower chassis is fixedly connected below the petri dish platform. There is a motor arranged inside the lower chassis, and the motor is electrically connected to the wire routing column and the marking head. A biochemical filter material replacement port is arranged on the front face of the lower chassis, and an opening and closing plate is also arranged at the biochemical filter material replacement port. Above the biochemical filter material replacement port, there is a switch button, and the switch button is electrically connected to the motor. Chassis heat dissipation holes are also arranged on both side surfaces of the lower chassis; support anti-slip foot pads are arranged at the bottom of the lower chassis. There are several petri dish placement grooves with different specifications and shapes arranged on the petri dish placement plate. The marking head uses cold laser engraving technology for marking. There is a gap of 0.4 - 1.2 cm left between the petri dish platform and the lower chassis, and this gap serves as the discharge port for the petri dish area. A USB external interface and a power connection port are also arranged on the back of the lower chassis.
2. The device for non-toxic labeling of in vitro culture dishes in cell therapy according to claim 1, characterized in that: The upper shell is made of acrylic transparent material, and a layer of ultraviolet protection film is also arranged on its outer surface.