An automatic liquid storage station for cell production

Abstract

The present invention provides an automatic liquid storage station for cell production, including a liquid storage frame, a liquid storage bag weighing platform, a sterilization switching device, a liquid storage bag shaking device and a waste liquid collection device. The liquid storage frame and the liquid storage bag shaking device are connected and fixed, and a mixing shaking device is provided on the liquid storage bag shaking device, so as to achieve mixing and shaking of the liquid storage frame; the liquid storage frame is used to place the liquid storage bag; the liquid storage bag weighing platform is used to weigh the weight of the liquid storage bag and the liquid in the liquid storage frame; the waste liquid collection device collects the waste liquid in the cell factory into the waste liquid collection device through a peristaltic pump. The beneficial effects of this scheme are: realizing automated processing, cooperating with the operation of the robot, automatically taking and placing the liquid storage frame, and weighing and testing the distribution effect of the liquid; the shaking device can realize the shaking of the liquid storage bag at any angle and frequency, realize the mixing function of the liquid in the liquid storage bag, and prevent the cells from sinking; the automatic liquid storage station is equipped with a liquid suction and sterilization device, which can be switched according to the needs of the operating procedure.

Description

Technical Field

[0001] The invention relates to a storage liquid shaking and waste liquid collecting device, in particular to an automatic liquid storage table for cell production. Background Art

[0002] When adherent cells are cultured, they adhere to the surface of the culture dish and continue to proliferate until the cells cover the entire surface of the cell factory. At this time, the adherent cells can be digested from the cell factory.

[0003] In the process of cell culture subculture and collection, in order to ensure the uniformity of operation, the cell suspension of a batch of culture must first be collected into a liquid storage bag, and the liquid storage bag is used as a relay for subsequent operations. At the same time, the operating liquid of the cells in the centrifuge bottle needs to be returned to the liquid storage bag for uniformity operation. In the application scenario of automatic cell production, there are uncertainties in the automatic storage and weighing inspection of the liquid storage bag, which will cause uneven distribution of liquid, and problems such as waste liquid collection in the cell factory need to be improved.

[0004] Therefore, in response to the above technical problems, it is necessary to design a new device to realize the automation of the operation of the factory combined liquid storage table, centralized collection of waste and steam sterilization, etc., to reduce the risk of contamination, achieve uniformity of operation, prevent cell sinking, improve work efficiency and reduce costs. Summary of the invention

[0005] The purpose of the present invention is to provide a method for preventing cells from sinking, ensuring liquid mixing, and facilitating centralized collection of waste liquid in cell factories. To achieve the above purpose, the present invention proposes the following technical solutions:

[0006] An automatic liquid storage table for cell production, comprising a liquid storage frame, a liquid storage bag weighing table, a sterilization switching device and a peristaltic pump, and also comprising a liquid storage bag shaking device and a waste liquid collection device. The liquid storage frame and the liquid storage bag shaking device are connected and fixed, and a mixing shaking device is arranged on the liquid storage bag shaking device, so as to achieve mixing and shaking of the liquid storage frame;

[0007] The interior of the liquid storage frame is used for placing the liquid storage bag;

[0008] The liquid storage bag weighing platform is used to weigh the weight of the liquid storage bag and the liquid in the liquid storage frame;

[0009] The waste liquid collection device is provided with a waste liquid collection frame, and the waste liquid in the cell factory is collected into the waste liquid collection device through a peristaltic pump;

[0010] The peristaltic pump plays a role of transfer. When adding liquid, the liquid in the liquid storage frame is transferred to the cell factory through the peristaltic pump; when collecting waste liquid, the waste liquid in the cell factory is transferred to the waste liquid collection device through the peristaltic pump.

[0011] A further improvement is that the liquid storage frame is a mechanical snap-on frame, and the front and rear ends of the liquid storage frame are respectively provided with a fixed plate and a robot gripper plate at the side ends, and the robot can pick up and place the liquid storage frame through the robot gripper plate.

[0012] The liquid storage bag shaking device comprises a fixed platform, a chuck, a reducer and a motor. The fixed platform is fixed on the liquid storage table. A through hole is provided on the fixed platform. One side of the chuck is connected to the reducer, and the other side is connected and fixed to the fixed plate through the through hole, so as to realize the connection and fixation between the liquid storage bag shaking device and the liquid storage frame.

[0013] Specifically, the fixing plate is configured as a circular bottom plate and a protrusion, and the protrusion passes through the chuck and enables the circular bottom plate to be fitted with the surface of the chuck.

[0014] The sterilization switching device includes a sterilization pipe joint, a stepper motor, a rocker slider structure and slideways arranged on both sides. A pair of circular notches are provided on the rocker slider structure. The rocker slider structure includes a rocker rod and sliders on both sides. A circular opening is opened on the upper plate of the sterilization switching device. The output shaft of the stepper motor passes through the circular opening and is connected to the rocker rod, so as to realize circular motion with the output shaft of the stepper motor as a fixed point, thereby driving the slider and the rocker slider structure to move back and forth on the slideway.

[0015] The waste liquid collection device adopts a drawer-type structure, with a robot handle on the side, and the drawer extension and retraction is completed by the robot;

[0016] It includes a liquid adding frame and a pipe plugging and pulling device. A brushless motor is installed in the pipe plugging and pulling device. A liquid adding pipe is arranged at the upper end of the liquid adding frame. A sterilization pipe is arranged on the pipe plugging and pulling device. The pipe plugging and pulling device includes a base plate, a rotating shaft and a movable plate. The base plate is fixed on the brushless motor. A sterilization pipe is arranged on the movable plate. The output shaft of the brushless motor is connected with the rotating shaft. When the brushless motor is started, the movable plate is driven to switch to the liquid adding pipe and the sterilization pipe respectively to complete the switching and plugging and pulling actions of the liquid adding and sterilization points.

[0017] It can be seen from the above technical solutions that the technical solutions of the present invention provide the following beneficial effects:

[0018] First, realize automated processing, cooperate with the robot operation, automatically pick up and place the liquid storage frame, weigh it, and check the liquid distribution effect;

[0019] Second, by setting the liquid storage frame and the shaking device, the liquid storage bag can be shaken at any angle and frequency to achieve the function of mixing the liquid in the liquid storage bag and prevent the cells from sinking;

[0020] Third, it is equipped with a waste liquid collection device, which can collect waste liquid from cell production according to program requirements;

[0021] Fourthly, the automatic liquid storage table is equipped with liquid adding and sterilization devices, which can be switched according to the needs of the operating procedures.

[0022] It should be appreciated that all combinations of the foregoing concepts, as well as additional concepts described in greater detail below, may be considered to be part of the inventive subject matter of the present disclosure, provided such concepts are not mutually inconsistent.

[0023] The foregoing and other aspects, embodiments and features of the present invention can be more fully understood from the following description in conjunction with the accompanying drawings. Other additional aspects of the present invention, such as the features and / or beneficial effects of the exemplary embodiments, will be apparent from the following description or learned from the practice of the specific embodiments according to the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component shown in various figures may be represented by the same reference numeral. For clarity, not every component is labeled in every figure. Embodiments of various aspects of the present invention will now be described by way of example and with reference to the accompanying drawings, in which:

[0025] Attached Figure 1 This is a schematic diagram of the structure of this scheme;

[0026] Attached Figure 2 For attachment Figure 1 Side view of

[0027] Attached Figure 3 It is a structural schematic diagram of a liquid storage frame;

[0028] Attached Figure 4 For attachment Figure 3 Side view of

[0029] Attached Figure 5 It is a structural schematic diagram of the liquid storage bag shaking device;

[0030] Attached Figure 6 It is a structural schematic diagram of the sterilization switching device;

[0031] Attached Figure 7 It is a structural schematic diagram of the upper swing rod of the sterilization switching device;

[0032] Attached Figure 8 It is a schematic diagram of the structure of the waste liquid collection device;

[0033] Attached Fig. 9 For attachment Figure 8 The structural diagram of part A in the figure;

[0034] Among them, 1-liquid storage frame, 2-liquid storage bag shaking device, 3-liquid storage bag weighing platform, 4-sterilization switching device, 5-waste liquid collection device, 6-peristaltic pump, 11-fixed plate, 12-liquid collection pipeline, 21-chuck, 22-reducer, 23-motor, 24-through port, 25-fixed platform, 41-sterilization pipeline joint, 42-stepping motor, 43-rocker slider structure, 44-slide, 431-rocker, 45-slide, 51-liquid adding frame, 52-sterilization pipeline, 53-liquid adding pipeline, 54-brushless motor, 55-bottom plate, 56-rotating shaft, 57-moving plate. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the embodiment of the present invention clearer, the technical solution of the embodiment of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiment of the present invention. Obviously, the described embodiment is a part of the embodiment of the present invention, not all of the embodiments. Based on the described embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein should be the common meanings understood by people with ordinary skills in the field to which the present invention belongs.

[0036] The words "first", "second" and similar words used in the patent application specification and claims of the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, unless the context clearly indicates otherwise, the singular form of "a", "an" or "the" and similar words do not indicate a quantity limitation, but indicate the existence of at least one. "Include" or "comprise" and similar words mean that the elements or objects appearing before "include" or "comprise" include the features, wholes, steps, operations, elements and / or components listed after "include" or "comprise", and do not exclude the existence or addition of one or more other features, wholes, steps, operations, elements, components and / or their collections. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0037] Example 1, refer to the attached Figure 1-2 The automatic liquid storage station for cell production in this scheme includes a liquid storage frame 1, a liquid storage bag weighing platform 3, a sterilization switching device 4 and a peristaltic pump 6; the peristaltic pump is configured with a single-head structure, the peristaltic pump rotation is driven by a 100W servo motor and a 1:5 reducer, the opening and closing of the pump head is driven by a 24V DC brushless motor, and an opening and closing position switch is configured as the zero position.

[0038] See attached Figure 3The liquid storage frame 1 is a frame body, which is fixed by a mechanical buckle structure. The liquid storage frame can be opened or closed. The liquid storage frame 1 is used to place a liquid storage bag. The upper end of the liquid storage frame 1 is provided with a liquid collection pipe 12. The front and rear ends of the liquid storage frame 1 are respectively provided with a fixing plate 11 and a robot gripper plate. The liquid storage frame 1 is automatically placed on the liquid storage bag shaking device by the robot to achieve fixation with the liquid storage bag shaking device. The liquid collection pipe 12 at the upper end of the liquid storage frame 1 is connected to the liquid storage frame 1 to achieve liquid addition.

[0039] Embodiment 2, the fixing method of the liquid storage frame 1 and the liquid storage bag shaking device 2 is detailed in the attached Figure 4-5 The fixed disk 11 at the side end of the liquid storage frame 1 includes a circular bottom plate and a protrusion; the bottom of the fixed platform 25 on the liquid storage bag shaking device 2 is fixed on the liquid storage table, and a through hole 24 is provided on the fixed platform 25. It also includes a chuck 21, a reducer 22 and a motor 23. One side of the chuck 21 is connected to the reducer 22, and the other side is connected and fixed to the fixed disk 11 through the through hole 24. Specifically, the protrusion on the fixed disk 11 passes through the middle hollow position of the chuck 21, so that the circular bottom plate on the fixed disk 11 is fitted with the surface of the chuck 21, so as to achieve a tightening effect. The liquid storage bag shaking device 2 is driven by a 40w motor and a reducer with a reduction ratio of 30 to achieve uniformity of operation. The shaking can achieve shaking of the liquid storage bag at any angle and frequency, and realize the mixing function of the liquid in the liquid storage bag.

[0040] A position-limiting laser detection sensor is disposed on the fixed platform 25, and the position-limiting laser detection sensor is a device for detecting whether the liquid storage frame is provided.

[0041] The liquid storage bag weighing platform 3 is used to weigh the weight of the liquid storage bag and the liquid in the liquid storage frame 1. The robot can automatically place the liquid storage frame 1 on the liquid storage bag weighing platform 3 through the robot gripper plate at the front end of the liquid storage frame 1. After weighing the entire weight and then removing the zero, the weight of the liquid in the liquid storage bag can be known to check the liquid distribution effect. The liquid storage bag weighing platform 3 is configured with a weighing sensor and an FD-4 weighing module, and the output signal is 4-20mA. A control cabinet is provided at the lower end of the device. The weighing sensor is actually a device that converts a mass signal into a measurable electrical signal output, and cooperates with the control cabinet to achieve automatic control.

[0042] Example 3, refer to the attached Figure 6-7 The sterilization switching device 4 includes a sterilization pipe joint 41, a stepping motor 42, a swing bar slider structure 43 and slideways 45 arranged on both sides. The swing bar slider structure 43 is provided with a pair of circular notches, the purpose of which is that when the swing bar slider structure 43 moves to the sterilization pipe joint 41, the circular notches can just play a role of clamping and fixing with the sterilization pipe joint 41. The sterilization pipe switching adopts a robot to insert the pipeline into the sterilization pipe joint.

[0043] The sterilization switching device is provided with a sterilization pipe plugging and unplugging structure, which is realized by a stepper motor 42 and a swing bar slider structure 43. Specifically, the swing bar slider structure 43 includes a swing bar 431 and sliders on both sides. The upper plate of the sterilization switching device 4 is provided with a circular opening, and the output shaft of the stepper motor 42 passes through the circular opening and is connected to the swing bar 431, so that the swing bar 431 can move in a circular motion with the output shaft of the stepper motor 42 as a fixed point, thereby driving the slider and the swing bar slider structure 43 to move forward and backward on the slideway 45.

[0044] The sterilization pipe plug-in is also equipped with two slot-type photoelectric switches for plug-in lock zero position and in position, which are arranged on one side of the sterilization switching device 4 and are provided with a paddle, one end of which is connected to the slot-type photoelectric switch, and the other side is arranged on the swing bar slider structure 43, which receives and converts the infrared light between the light emitter and the light receiver to detect the position of the object and fix it. The sterilization switching device 4 is also provided with a detection device, which is a reflective optical fiber sensor, used to detect the position of the sterilization pipe joint 41.

[0045] Refer to the attached Figure 8-9 The waste liquid collection device 5 is provided with a waste liquid collection frame, and the waste liquid in the cell factory is collected into the waste liquid collection device 5 through the peristaltic pump 6. The waste liquid collection device 5 adopts a drawer structure, and a robot handle is provided at the side end of the waste liquid collection device. The drawer is extended and retracted by the robot, and the drawer is also equipped with an extension limit switch and a mechanical positioning device.

[0046] The waste liquid collection device 5 includes a liquid adding frame 51 and a pipe plug-in device. A brushless motor 54 is arranged inside the pipe plug-in device. A liquid adding pipe 53 is arranged at the upper end of the liquid adding frame 51. The pipe plug-in device includes a base plate 55, a rotating shaft 56 and a movable plate 57. The base plate 55 is fixed on the brushless motor 54. A sterilization pipe 52 is arranged on the movable plate 57. The output shaft of the brushless motor 54 is connected to the rotating shaft 56. When the brushless motor 54 is started, the movable plate 57 is driven to switch to the liquid adding pipe and the sterilization pipe respectively, thereby completing the switching and plug-in actions of the liquid adding and sterilization points.

[0047] Workflow: When adding liquid, the liquid in the liquid storage frame 1 is connected to the peristaltic pump through the liquid collection pipe 12 at the upper end, and then transported to the cell factory; when collecting waste liquid, the waste liquid in the cell factory is connected to the liquid adding pipe 53 through the peristaltic pump, and then reaches the waste liquid collection device 5; during sterilization, steam comes out of the steam generator, through the sterilization pipe connector 41 at the upper end of the sterilization switching device, connected to the sterilization pipe 52 on the waste liquid collection device 5, and then reaches the subsequent device.

[0048] Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. A person with ordinary knowledge in the technical field to which the present invention belongs may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be determined by the definition of the claims.

Claims

1. An automatic liquid storage table for cell production, comprising a liquid storage frame (1), a liquid storage bag weighing table (3), a sterilization switching device (4) and a peristaltic pump (6), characterized in that: It also includes a liquid storage bag shaking device (2) and a waste liquid collection device (5); the liquid storage frame (1) and the liquid storage bag shaking device (2) are connected and fixed, and the liquid storage bag shaking device (2) is provided with a mixing and shaking device, so that the liquid storage frame (1) can be mixed and shaken; The liquid storage frame (1) is used to place the liquid storage bag inside; The liquid storage bag weighing platform (3) is used to weigh the weight of the liquid storage bag and the liquid in the liquid storage frame (1); A waste liquid collection frame is provided in the waste liquid collection device (5), and waste liquid in the cell factory is collected into the waste liquid collection device (5) via a peristaltic pump (6); The liquid storage frame (1) is a frame body, the upper end of the liquid storage frame (1) is provided with a liquid collecting pipe (12), and the front and rear ends of the liquid storage frame (1) are respectively provided with a fixing plate (11) and a robot gripper plate; The liquid storage bag shaking device (2) comprises a fixed platform (25), a chuck (21), a reducer (22) and a motor (23); the fixed platform (25) is fixed on the liquid storage table; a through hole (24) is provided on the fixed platform (25); one side of the chuck (21) is connected to the reducer (22); the other side of the chuck (21) passes through the through hole (24) and is connected and fixed to the fixed plate (11), thereby realizing the connection and fixation of the liquid storage bag shaking device (2) and the liquid storage frame (1); wherein the liquid storage bag shaking device (2) is driven by a 40W motor and a reducer with a reduction ratio of 30.

2. The automatic liquid storage station for cell production according to claim 1, characterized in that: The sterilization switching device (4) comprises a sterilization pipe joint (41), a stepping motor (42), a swing rod slider structure (43) and slideways (45) arranged on both sides. The swing rod slider structure (43) is provided with a pair of circular notches. The plugging and unplugging of the sterilization pipe is achieved by the stepping motor (42) and the swing rod slider structure (43). The swing rod slider structure (43) comprises a swing rod (431) and sliders on both sides. The upper plate of the sterilization switching device (4) is provided with a circular opening. The output shaft of the stepping motor (42) passes through the circular opening and is connected to the swing rod (431). The swing rod (431) can realize circular motion with the output shaft of the stepping motor (42) as a fixed point, thereby driving the slider and the swing rod slider structure (43) to move forward and backward on the slideway (45). The waste liquid collection device (5) comprises a liquid adding frame (51) and a pipe plugging and pulling device. A brushless motor (54) is arranged in the pipe plugging and pulling device. A liquid adding pipe (53) is arranged at the upper end of the liquid adding frame (51). A sterilization pipe is arranged on the pipe plugging and pulling device. The brushless motor (54) is started to drive the pipe plugging and pulling device to operate.

3. The automatic liquid storage station for cell production according to claim 2, characterized in that: The fixing plate (11) is configured as a circular bottom plate and a protrusion, wherein the protrusion passes through the chuck (21) and enables the circular bottom plate to be arranged in close contact with the surface of the chuck (21).

4. The automatic liquid storage station for cell production according to claim 3, characterized in that: The pipeline plugging and unplugging device comprises a bottom plate (55), a rotating shaft (56) and a moving plate (57); the bottom plate (55) is fixed on a brushless motor (54); a sterilizing pipeline (52) is provided on the moving plate (57); an output shaft of the brushless motor (54) is connected to the rotating shaft (56); when the brushless motor (54) is started, the moving plate (57) is driven to switch to the liquid adding pipeline and the sterilizing pipeline respectively, thereby completing the switching and plugging and unplugging actions of the liquid adding and sterilizing points.

5. The automatic liquid storage station for cell production according to claim 4, characterized in that: A robot handle is provided at the side end of the waste liquid collection device (5), and the extension and retraction of the drawer is completed by the robot.

6. The automatic liquid storage station for cell production according to claim 5, characterized in that: When adding liquid, the liquid in the liquid storage frame (1) is transferred to the cell factory through the peristaltic pump; when collecting waste liquid, the waste liquid in the cell factory is transferred to the waste liquid collection device (5) through the peristaltic pump.

7. The automatic liquid storage station for cell production according to claim 6, characterized in that: The liquid storage bag weighing platform (3) is equipped with a weighing sensor and a weighing module for control.

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