Method for rapidly identifying purity of rape hybrids indoors

Abstract

The invention discloses a method for rapidly identifying the purity of rape hybrids indoors. The method comprises the following step of sterilizing rape seeds, specifically, soaking the hybrid seeds with a 0.1% mercuric chloride solution for 10-15 minutes, cleaning the seeds with ddH2O for 2-3 times, carrying out low-temperature vernalization in a bud stage, putting the sterilized seeds into a culture dish paved with three layers of wet filter paper, putting the culture dish into an artificial climate chamber, placing the seeds at normal temperature for 1-2 days until the seeds germinate, putting the germinated seeds exposing white buds into an incubator at 2 DEG C or 4 DEG C, and carrying out low-temperature treatment for 2-3 weeks; and through artificial low-temperature vernalization, increase of the planting density in a seedling stage and increase of the temperature, sunshine duration and illumination intensity in the growth stage, the growth process is effectively accelerated, and the purpose of rapidly identifying the purity of the hybrids is achieved. The method has the beneficial effects that the growth process is effectively accelerated and the purity of the rape hybrids is rapidly identified through artificial low-temperature vernalization treatment of the rape seeds, coordinated control over the temperature, illumination time and illumination intensity and technical measures such as the increase of the planting density, topping and branch reduction.

Description

technical field

[0001] The invention relates to a method for rapidly identifying the purity of rapeseed hybrids, in particular to a method for rapidly identifying the purity of rapeseed hybrids indoors, and belongs to the application technical field of methods for quickly identifying the purity of hybrids of Brassica napus indoors. Background technique

[0002] Seed purity is the core indicator for identifying the quality of hybrid seeds, and is directly related to the quality and yield of hybrids in expanded production. The field planting identification of Brassica napus hybrids is affected by the season, and usually requires 1-2 growth cycles, which is difficult to meet the needs of seed supply in actual production, and seriously delays the time period for the identification of the purity of Brassica napus hybrids. Contents of the invention

[0003] The purpose of the present invention is to solve the above-mentioned problems, and propose a kind of method of rapid identi...

Examples

Embodiment 1

[0019] A method for rapid indoor identification of the purity of rapeseed hybrids, the method specifically comprising the following steps:

[0020] Step 1: Sampling. After the hybrids on the female plants enter the yellow ripening stage, select representative plants to harvest 5 days in advance, dry in the sun, and thresh. Duration: 1 day;

[0021] Step 2: Sterilize the hybrid seeds, soak the rape seeds with 0.1% mercuric chloride solution for 15 minutes, and use ddH 2 O wash the seeds 3 times;

[0022] Step 3: Low-temperature vernalization treatment at the germination stage. Put the sterilized seeds into a petri dish covered with three layers of moist filter paper, place them in an artificial climate chamber, and place them at room temperature for 2 days until the seeds germinate. Put them into an incubator at 2°C for low temperature treatment for 2 weeks. Duration: 14 days;

[0023] Step 4: Transplanting. The low-temperature-treated seeds are slowly seeded indoors for 1 ...

Embodiment 2

[0028] A method for rapid indoor identification of the purity of rapeseed hybrids, the method specifically comprising the following steps:

[0029] Step 1: Sampling. After the hybrids on the female plants enter the yellow ripening stage, select representative plants to harvest 7 days in advance, dry in the sun, and thresh. Duration: 1 day.

[0030] Step 2: Sterilize the hybrid seeds, soak the rape seeds with 0.1% mercuric chloride solution for 10 minutes, and use ddH 2 O wash the seeds 2 times.

[0031] Step 3: Low-temperature vernalization treatment at the germination stage. Put the sterilized seeds into a petri dish covered with three layers of moist filter paper, place them in an artificial climate chamber, and place them at room temperature for 2 days until the seeds germinate. Place them in a 4°C incubator for 3 weeks at low temperature. Duration: 21 days;

[0032] Step 4: Transplanting, the seeds after low temperature treatment are slowly seeded indoors for 1 day, an...

Embodiment 3

[0037] A method for rapid indoor identification of the purity of rapeseed hybrids, the method specifically comprising the following steps:

[0038] Step 1: Sampling. After the hybrids on the female plants enter the yellow ripening stage, select representative plants to harvest 5 days in advance, dry in the sun, and thresh. Duration: 1 day;

[0039] Step 2: Sterilize the hybrid seeds, soak the rapeseed seeds with 0.1% mercuric chloride solution for 12min, and wash them with ddH 2 O wash the seeds 2 times.

[0040] Step 3: Low-temperature vernalization treatment at the germination stage. Put the sterilized seeds into a petri dish covered with three layers of moist filter paper, place them in an artificial climate chamber, and place them at room temperature for 1 day until the seeds germinate. Put them into an incubator at 4°C for low temperature treatment for 2-3 weeks. Duration: 18 days;

[0041] Step 4: Transplanting, the seeds after low temperature treatment are slowly se...

technical field

[0001] The invention relates to a method for rapidly identifying the purity of rapeseed hybrids, in particular to a method for rapidly identifying the purity of rapeseed hybrids indoors, and belongs to the application technical field of methods for quickly identifying the purity of hybrids of Brassica napus indoors. Background technique

[0002] Seed purity is the core indicator for identifying the quality of hybrid seeds, and is directly related to the quality and yield of hybrids in expanded production. The field planting identification of Brassica napus hybrids is affected by the season, and usually requires 1-2 growth cycles, which is difficult to meet the needs of seed supply in actual production, and seriously delays the time period for the identification of the purity of Brassica napus hybrids. Contents of the invention

[0003] The purpose of the present invention is to solve the above-mentioned problems, and propose a kind of method of rapid identi...

Examples

Embodiment 1

[0019] A method for rapid indoor identification of the purity of rapeseed hybrids, the method specifically comprising the following steps:

[0020] Step 1: Sampling. After the hybrids on the female plants enter the yellow ripening stage, select representative plants to harvest 5 days in advance, dry in the sun, and thresh. Duration: 1 day;

[0021] Step 2: Sterilize the hybrid seeds, soak the rape seeds with 0.1% mercuric chloride solution for 15 minutes, and use ddH 2 O wash the seeds 3 times;

[0022] Step 3: Low-temperature vernalization treatment at the germination stage. Put the sterilized seeds into a petri dish covered with three layers of moist filter paper, place them in an artificial climate chamber, and place them at room temperature for 2 days until the seeds germinate. Put them into an incubator at 2°C for low temperature treatment for 2 weeks. Duration: 14 days;

[0023] Step 4: Transplanting. The low-temperature-treated seeds are slowly seeded indoors for 1 ...

Embodiment 2

[0028] A method for rapid indoor identification of the purity of rapeseed hybrids, the method specifically comprising the following steps:

[0029] Step 1: Sampling. After the hybrids on the female plants enter the yellow ripening stage, select representative plants to harvest 7 days in advance, dry in the sun, and thresh. Duration: 1 day.

[0030] Step 2: Sterilize the hybrid seeds, soak the rape seeds with 0.1% mercuric chloride solution for 10 minutes, and use ddH 2 O wash the seeds 2 times.

[0031] Step 3: Low-temperature vernalization treatment at the germination stage. Put the sterilized seeds into a petri dish covered with three layers of moist filter paper, place them in an artificial climate chamber, and place them at room temperature for 2 days until the seeds germinate. Place them in a 4°C incubator for 3 weeks at low temperature. Duration: 21 days;

[0032] Step 4: Transplanting, the seeds after low temperature treatment are slowly seeded indoors for 1 day, an...

Embodiment 3

[0037] A method for rapid indoor identification of the purity of rapeseed hybrids, the method specifically comprising the following steps:

[0038] Step 1: Sampling. After the hybrids on the female plants enter the yellow ripening stage, select representative plants to harvest 5 days in advance, dry in the sun, and thresh. Duration: 1 day;

[0039] Step 2: Sterilize the hybrid seeds, soak the rapeseed seeds with 0.1% mercuric chloride solution for 12min, and wash them with ddH 2 O wash the seeds 2 times.

[0040] Step 3: Low-temperature vernalization treatment at the germination stage. Put the sterilized seeds into a petri dish covered with three layers of moist filter paper, place them in an artificial climate chamber, and place them at room temperature for 1 day until the seeds germinate. Put them into an incubator at 4°C for low temperature treatment for 2-3 weeks. Duration: 18 days;

[0041] Step 4: Transplanting, the seeds after low temperature treatment are slowly se...