Preparation method and application of rhamnolipid composite surfactant

Abstract

The invention belongs to the technical field of surfactants, and discloses a preparation method and application of a rhamnolipid composite surfactant. The preparation method comprises the following steps: adding lysozyme into rhamnolipid fermentation liquor which is totally fermented, conducting stirring and reacting under the conditions that a temperature is 45-50 DEG C and a pH value is 5-7, cooling the obtained fermentation liquor to room temperature, then adding APG, carrying out stirring and washing, then adding polyaluminum chloride, carrying out flocculent precipitation and centrifugation, taking a supernatant, and concentrating and drying the supernatant to obtain the rhamnolipid-APG composite surfactant. According to the preparation method disclosed by the invention, lysozyme is used for treating the fermentation liquor, and APG is used for washing, so the yield of rhamnolipid can be remarkably increased; and polymeric aluminum chloride is adopted to carry out flocculent precipitation on the fermentation liquor having been subjected to bacteriolysis treatment, so the purity of the product is improved. The obtained product has good surface activity, and has the advantages of being natural, environment-friendly, non-irritant and biodegradable when being applied to daily chemical washing products.

Description

technical field

[0001] The invention belongs to the technical field of surfactants, in particular to a preparation method and application of a rhamnolipid composite surfactant. Background technique

[0002] Rhamnolipid is a biosurfactant with the longest research time and the most mature application technology. It is mainly produced by fermentation by Pseudomonas or Burkholderia species or other suitable microorganisms. However, the resulting fermentation mixture or "broth" must be further processed to isolate / purify the rhamnolipids. The fermentation broth of rhamnolipid-producing bacteria has a complex composition. Other fermentation broth components include incompletely reacted feed substrates, such as triglycerides, diglycerides, and monoglycerides, as well as fatty acids, as well as cellular components and sugars. Extracting rhamnolipids from fermentation broth is costly and difficult. High recovery and purification costs are the main obstacles to be overcome for the...

Examples

Embodiment 1

[0032] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:

[0033] (1) add lysozyme in the rhamnolipid fermented liquid, the add-on of regulating lysozyme is respectively 0.01%, 0.02%, 0.04%, 0.08% and 0.10% of the rhamnolipid fermented liquid quality, at a temperature of 45 ~50°C, stirring and reacting for 2 hours under the condition that phosphoric acid adjusts the pH to 5.5-6.5. And the fermentation broth without adding lysozyme was used as blank control.

[0034] (2) Cool the fermented liquid after the lysing treatment in step (1) to room temperature, then add APG to stir and wash, and the amount of APG added is 3.5% of the mass of the fermented liquid after the lysing process.

[0035] (3) adding polyaluminium chloride to the fermented liquid after step (2) washing carries out flocculation precipitation, and the flocculation precipitation maintenance system pH is about 6, and the add-on of polya...

Embodiment 2

[0041] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:

[0042] (1) Add lysozyme to the rhamnolipid fermentation liquid, the addition of lysozyme is 0.04% of the quality of the rhamnolipid fermentation liquid, under the condition that the temperature is 45~50° C. and the pH adjusted by phosphoric acid is 5.5~6.5 The reaction was stirred for 2h.

[0043] (2) cooling the fermented liquid after step (1) lysing treatment to room temperature, then adding APG to stir and wash, adjusting the addition of APG to be respectively 0.5%, 1%, 2%, 3.5% of the fermented liquid mass after lysing treatment , 5% and 7.5%. And the fermentation broth without adding APG was used as blank control.

[0044] (3) adding polyaluminium chloride to the fermented liquid after step (2) washing carries out flocculation precipitation, and the flocculation precipitation maintenance system pH is about 6, and the add-on of polyalum...

Embodiment 3

[0050] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:

[0051] (1) Add lysozyme to the rhamnolipid fermentation liquid, the addition of lysozyme is 0.04% of the quality of the rhamnolipid fermentation liquid, under the condition that the temperature is 45~50° C. and the pH adjusted by phosphoric acid is 5.5~6.5 The reaction was stirred for 2h.

[0052] (2) Cool the fermented liquid after the lysing treatment in step (1) to room temperature, then add APG to stir and wash, and the amount of APG added is 3.5% of the mass of the fermented liquid after the lysing process.

[0053] (3) Add polyaluminium chloride to the fermented liquid after step (2) washing and carry out flocculation precipitation, flocculation precipitation maintenance system pH is about 6, adjusts the add-on of polyaluminium chloride respectively to be 0.05 of the fermented liquid quality after washing %, 0.1%, 0.2%, 0.35%, 0.5% and...

technical field

[0001] The invention belongs to the technical field of surfactants, in particular to a preparation method and application of a rhamnolipid composite surfactant. Background technique

[0002] Rhamnolipid is a biosurfactant with the longest research time and the most mature application technology. It is mainly produced by fermentation by Pseudomonas or Burkholderia species or other suitable microorganisms. However, the resulting fermentation mixture or "broth" must be further processed to isolate / purify the rhamnolipids. The fermentation broth of rhamnolipid-producing bacteria has a complex composition. Other fermentation broth components include incompletely reacted feed substrates, such as triglycerides, diglycerides, and monoglycerides, as well as fatty acids, as well as cellular components and sugars. Extracting rhamnolipids from fermentation broth is costly and difficult. High recovery and purification costs are the main obstacles to be overcome for the...

Examples

Embodiment 1

[0032] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:

[0033] (1) add lysozyme in the rhamnolipid fermented liquid, the add-on of regulating lysozyme is respectively 0.01%, 0.02%, 0.04%, 0.08% and 0.10% of the rhamnolipid fermented liquid quality, at a temperature of 45 ~50°C, stirring and reacting for 2 hours under the condition that phosphoric acid adjusts the pH to 5.5-6.5. And the fermentation broth without adding lysozyme was used as blank control.

[0034] (2) Cool the fermented liquid after the lysing treatment in step (1) to room temperature, then add APG to stir and wash, and the amount of APG added is 3.5% of the mass of the fermented liquid after the lysing process.

[0035] (3) adding polyaluminium chloride to the fermented liquid after step (2) washing carries out flocculation precipitation, and the flocculation precipitation maintenance system pH is about 6, and the add-on of polya...

Embodiment 2

[0041] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:

[0042] (1) Add lysozyme to the rhamnolipid fermentation liquid, the addition of lysozyme is 0.04% of the quality of the rhamnolipid fermentation liquid, under the condition that the temperature is 45~50° C. and the pH adjusted by phosphoric acid is 5.5~6.5 The reaction was stirred for 2h.

[0043] (2) cooling the fermented liquid after step (1) lysing treatment to room temperature, then adding APG to stir and wash, adjusting the addition of APG to be respectively 0.5%, 1%, 2%, 3.5% of the fermented liquid mass after lysing treatment , 5% and 7.5%. And the fermentation broth without adding APG was used as blank control.

[0044] (3) adding polyaluminium chloride to the fermented liquid after step (2) washing carries out flocculation precipitation, and the flocculation precipitation maintenance system pH is about 6, and the add-on of polyalum...

Embodiment 3

[0050] A kind of preparation method of rhamnolipid complex surfactant of the present embodiment comprises following preparation steps:

[0051] (1) Add lysozyme to the rhamnolipid fermentation liquid, the addition of lysozyme is 0.04% of the quality of the rhamnolipid fermentation liquid, under the condition that the temperature is 45~50° C. and the pH adjusted by phosphoric acid is 5.5~6.5 The reaction was stirred for 2h.

[0052] (2) Cool the fermented liquid after the lysing treatment in step (1) to room temperature, then add APG to stir and wash, and the amount of APG added is 3.5% of the mass of the fermented liquid after the lysing process.

[0053] (3) Add polyaluminium chloride to the fermented liquid after step (2) washing and carry out flocculation precipitation, flocculation precipitation maintenance system pH is about 6, adjusts the add-on of polyaluminium chloride respectively to be 0.05 of the fermented liquid quality after washing %, 0.1%, 0.2%, 0.35%, 0.5% and...