A volatile organic polluted soil biological toxicity testing device and its use method

Abstract

The present invention provides a volatile organic polluted soil biological toxicity testing device and a method for using the same. The device consists of a soil sample bottle, a gas washing and testing device bottle, and a peristaltic pump. The top of the gas washing and testing device bottle is connected to the bottom of the soil sample bottle through a conduit, and a peristaltic pump is provided on the conduit. The conduit connected above the soil sample bottle passes through the conduit socket of the gas washing and testing device bottle body and is inserted into the bottom of the bottle. The device siphons a solution containing volatile pollutants into a sponge through a capillary tube, maintains the sponge moist, ensures the humid environment conditions required for springtails, and tests springtail avoidance behavior at the same time. The device can simultaneously test the effects of volatile pollutants in air and aqueous solutions on springtails, and has high sensitivity.

Description

Technical Field

[0001] The invention relates to a volatile organic polluted soil biological toxicity testing device and a use method thereof, belonging to the field of soil biological comprehensive toxicity testing methods targeting soil habitat functions. Background Art

[0002] As a special type of soil pollutants, volatile organic compounds are listed as pollutants with high potential risks in the environment and should be controlled first due to the complexity and harmfulness of their components. The pollution diagnosis of site soil can provide an important basis for site management decisions. At present, soil pollution diagnosis in my country is mainly based on pollutant concentration analysis, which has the disadvantages of high testing costs, direct impact of preset testing indicators on test results, and inability to reflect the comprehensive toxicity of complex multi-component pollutants.

[0003] Using sensitive organisms to directly reflect the comprehensive toxic effects of contaminated sites can characterize the comprehensive toxic effects of soil samples on biological individuals and populations. It is simple and economical, and can screen contaminated soils of multiple batches, multiple types, and different concentrations. However, existing testing methods are only applicable to non-volatile organic pollution and heavy metal pollution sites, and are not applicable to volatile organic polluted soils.

[0004] The white-tailed flounder is a representative of sensitive organisms in the soil ecosystem and is widely used in environmental hazard assessments of pesticides, contaminated soil, etc. Since the white-tailed flounder has a posterior horn at the base of its antennae, it can be used to monitor chemicals in the air. There is a pair of abdominal tubes on the ventral surface of the first abdominal segment, which can exchange liquids with the external environment. Therefore, the white-tailed flounder is a good object for monitoring volatile organic pollutants. ISO has developed multiple test methods based on white-tailed flounders and other collembolans, but these methods are applicable to non-volatile organic pollution and heavy metal contaminated sites, not to volatile organic contaminated soils. Summary of the invention

[0005] The present invention aims to solve the above-mentioned deficiencies in the prior art and proposes a biological toxicity testing device suitable for volatile organic polluted soil.

[0006] In order to achieve the above purpose, this device is implemented through the following technical solutions:

[0007] The device consists of three parts: a soil sample bottle, a gas washing and test device bottle, and a peristaltic pump.

[0008] The soil sample bottle is a cylindrical glass bottle with upper and lower openings and a stopper, a catheter hole is arranged on the bottle stopper, and a sieve plate is arranged inside the bottle.

[0009] The gas washing and testing device bottle consists of a plugged upper cover and a bottle body, the bottle plug is provided with a catheter hole, the upper cover is provided with a glass partition, the bottle body is provided with a catheter insertion port, and the bottle body is provided with a glass partition and a capillary.

[0010] The top of the gas washing and testing device bottle is connected to the bottom of the soil sample bottle through a conduit, a peristaltic pump is arranged on the conduit, and the conduit connected to the top of the soil sample bottle passes through the conduit socket of the gas washing and testing device bottle body and is inserted into the bottom of the bottle.

[0011] Specifically, the upper opening of the soil sample bottle is larger than the lower opening, and the sieve plate is located near the bottom of the soil sample bottle.

[0012] Specifically, the glass partition inside the bottle body of the gas washing and testing device bottle is located at the upper part of the bottle body and parallel to the bottom of the bottle. The glass partition is in the shape of a superior arc, the curved edge is in contact with the bottle body, and the straight edge is provided with an airway partition perpendicular to the glass partition. The height of the airway partition is 20mm-30mm. Small holes are evenly distributed on the glass partition. The glass partition is divided into two areas by a horizontal partition. A sponge is placed on one area, and a capillary is inserted into the sponge. The capillary passes through the small holes on the glass partition and extends downward perpendicularly to the bottom surface. The other area is coated with oily glue that does not contain volatile substances.

[0013] Specifically, the glass partition in the upper cover of the gas washing and testing device bottle is parallel to the bottom of the bottle, and the bottom surface of the glass partition is coated with oily glue that does not contain volatile substances.

[0014] Specifically, the sponge is black and has a thickness of 5-10 mm.

[0015] Another object of the present invention is to provide a method for using the above-mentioned device, which comprises filling the soil to be tested on the sieve plate of the soil sample bottle; filling the washing and testing device bottle with deionized water as a washing solution, with the liquid level being lower than the glass partition in the bottle body; tightly closing all bottle stoppers, starting the peristaltic pump to draw the gas above the washing and testing device bottle into the bottom of the soil sample bottle, and then entering the washing solution in the washing and testing device bottle from the top, and waiting for the sponge on the glass partition to be moistened, pausing the peristaltic pump, opening the upper cover of the washing and testing device bottle, adding white springtail larvae to the sponge, closing the upper cover, starting the peristaltic pump, timing and recording the number of white springtails that jump out of the sponge and the number of springtails remaining on the sponge.

[0016] The volatile organic polluted soil biological toxicity test device of the present invention has the following advantages: the solution containing volatile pollutants siphoned into the sponge by the capillary tube can maintain the sponge's moisture to ensure the humid environment conditions required by the springtail on the one hand, and can also be a test solution for testing the springtail's avoidance behavior on the other hand. The hole in the 9A area can make the excess solution siphoned into the sponge leak back into the gas washing liquid. The influence of volatile pollutants in the air and aqueous solution on the springtail can be tested simultaneously, and the sensitivity is high. The oily glue without volatile substances is used to apply to the partial area, which can avoid the avoidance behavior of some springtails. After jumping out of the sponge, the springtails will again produce avoidance behavior and jump back to the sponge area due to the influence of volatile pollutants in the air. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 This is a schematic diagram of the bottle cover of the gas washing and test device;

[0019] Figure 3 This is a bottom view of the upper cover of the gas washing and test device bottle;

[0020] Figure 4 It is a schematic diagram of the bottle of the scrubbing and test device;

[0021] Figure 5 This is a top view of the gas scrubbing and test device bottle;

[0022] In the figure: 1- soil sample bottle, 2- soil sample containing volatile pollutants, 3- sieve plate, 4- silicone bottle stopper, 5- air guide tube, 6- peristaltic pump, 7- upper cover glass partition, 8- sponge, 9- bottle body glass partition, 10- capillary tube, 11- scrubbing solution, 12- scrubbing and test device bottle, 9A- sponge area of ​​bottle body glass partition, 9B- glue area of ​​bottle body glass partition, 9C- horizontal partition between sponge area and glue area, 9D- airway partition of bottle body glass partition. DETAILED DESCRIPTION

[0023] The specific implementation of the present invention will be described below with reference to the accompanying drawings.

[0024] A volatile organic polluted soil biological toxicity testing device, such as Figure 1 As shown, when using the device, a certain amount (such as 500 g) of soil to be tested is first filled in the soil sample bottle 1.

[0025] In the gas washing and testing device bottle 12, an oily glue without volatile substances is applied to the lower side of the upper cover glass partition 7. A sponge is placed on the bottle body glass partition 9 in area A, and an oily glue without volatile substances is applied to area B. A capillary is placed on the bottom of the sponge, and deionized water is filled at the bottom of the bottle as a gas washing solution, with the liquid level being lower than the bottle body glass partition 9.

[0026] Start the peristaltic pump 6 to allow the airflow to enter the bottom of the soil sample bottle 1 from the bottom, exit the soil sample bottle 1 from the top and enter the washing and test device bottle 12. After passing through the washing liquid, the airflow passes through the channel above the sponge 8 and exits the washing and test device bottle 12, and then returns to the soil sample bottle 1 through the peristaltic pump to enter the next cycle.

[0027] After running for 2 hours, observe through the washing and testing device bottle 12 that the sponge placed in area A on the glass partition 9 of the bottle body has been wetted. Pause the peristaltic pump 6, open the upper cover of the washing and testing device bottle 12, gently add N (30 recommended) 9-12 day old white-flecked larvae to the sponge, cover the upper cover of the washing and testing device bottle 12, start the peristaltic pump, and start timing. Observe for 15 minutes and record the number of white-flecked larvae that jump out of the sponge N1.

[0028] Continue running for 48 h and record the number of springtails N2 remaining on the sponge.

[0029] The calculation of the evasion rate of springtails is as follows:

[0030]

[0031] The volatile gas circulates in a closed system, which can gradually balance the distribution of volatile pollutants in the water, soil and air in the system. The solution containing volatile pollutants siphoned into the sponge through the capillary tube, on the one hand, maintains the sponge moist and ensures the humid environment conditions required for the springtail, and on the other hand, it is also a test solution for testing the avoidance behavior of springtails. When the volatile pollutants in the solution and airflow have a toxic effect on the springtail, the springtail will produce avoidance behavior and bounce to area B on the glass partition 9 of the bottle body. Since this area has been coated with oily glue, the springtail that jumps to this area will not be able to jump again. Using this device, the effects of volatile pollutants in the air and aqueous solution on the springtail can be tested simultaneously with high sensitivity.

[0032] The above is merely a basic implementation form of the present invention according to the technical solution. Any improvement of similar form proposed based on the present idea should be regarded as within the scope of protection of the present invention.

Claims

1. A volatile organic polluted soil biological toxicity testing device, characterized in that: The device consists of three parts: a soil sample bottle, a gas washing and test device bottle, and a peristaltic pump; The soil sample bottle is a cylindrical glass bottle with upper and lower openings and a stopper, a catheter hole is provided on the stopper, and a sieve plate is provided inside the bottle; The gas washing and testing device bottle is composed of a plugged upper cover and a bottle body, the bottle plug is provided with a catheter hole, the upper cover is provided with a glass partition, the bottle body is provided with a catheter insertion port, and the bottle body is provided with a glass partition and a capillary tube; The top of the gas washing and testing device bottle is connected to the bottom of the soil sample bottle through a conduit, the conduit is connected to a peristaltic pump, and the conduit connected to the top of the soil sample bottle passes through the conduit socket of the gas washing and testing device bottle body and is inserted into the bottom of the bottle; The glass partition inside the bottle body of the gas washing and testing device bottle is located at the upper part of the bottle body and parallel to the bottom of the bottle. The glass partition is in the shape of a superior arc, the curved edge is in contact with the bottle body, and the straight edge is provided with an airway partition perpendicular to the glass partition. The height of the airway partition is 20mm-30mm. Small holes are evenly distributed on the glass partition. The glass partition is divided into two areas by a horizontal partition. A sponge is placed on one area, and a capillary is inserted into the sponge. The capillary passes through the small hole on the glass partition and extends downward perpendicularly to the bottom surface. The other area is coated with oily glue that does not contain volatile substances.

2. The device according to claim 1, characterized in that The upper opening of the soil sample bottle is larger than the lower opening, and the sieve plate is located in the soil sample bottle near the bottom.

3. The device according to claim 1, characterized in that The glass partition in the upper cover of the gas washing and testing device bottle is semicircular and parallel to the bottom of the bottle, and the lower side of the glass partition is coated with oily glue without volatile substances.

4. The device according to claim 1, characterized in that The sponge is black and has a thickness of 5-10 mm.

5. A method for using the device according to any one of claims 1 to 4, characterized in that: Fill the soil to be tested on the sieve plate of the soil sample bottle; fill the washing and testing device bottle with deionized water as the washing solution, with the liquid level lower than the glass partition inside the bottle; cover all the bottle stoppers, start the peristaltic pump to draw the gas above the washing and testing device bottle into the bottom of the soil sample bottle, and then enter the washing solution in the washing and testing device bottle from the top, wait for the sponge to be moistened, pause the peristaltic pump, open the upper cover of the washing and testing device bottle, add white-letter springtail larvae to the sponge, cover the upper cover, start the peristaltic pump, time and record the number of white-letter springtails jumping out of the sponge and the number of springtails remaining on the sponge.

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