Aquatic fish immunopotentiator as well as preparation method and application of immunopotentiator

Abstract

The invention discloses an aquatic fish immunopotentiator as well as a preparation method and application thereof. The immunopotentiator comprises 10-25 parts of poria cocos and 10-25 parts of glossy privet fruits. The preparation process comprises the following steps: preliminarily crushing raw materials, carrying out three-step water extraction on the glossy privet fruits, carrying out rotary evaporation concentration, carrying out alcohol precipitation, and finally mixing the two Chinese herbal medicines according to a ratio of 1: 1, and the immunopotentiator can be used as a basal feed in the aquaculture fish culture process and can also be directly prepared into a compound Chinese herbal medicine feed. The compound immunopotentiator is proved to be capable of effectively enhancing the growth performance and immune function of aquatic fishes, the preparation method is simple and feasible, and the compound immunopotentiator has the advantages of low cost, low residue, low drug resistance and the like.

Description

technical field

[0001] The invention relates to an immune enhancer for aquaculture, which belongs to a compound preparation of Chinese herbal medicine, and specifically refers to a compound immune enhancer of Chinese herbal medicine, a preparation method and its application as an immune enhancer in aquatic fish farming. Background technique

[0002] In recent years, with the rapid development of my country's aquaculture industry. With the high yield and low cost of today's high-density and intensive aquaculture fish farming, excessive aquaculture density will directly bring a huge load to the water environment, and the fish body will also be stressed, reducing the resistance of fish to diseases , the physiological function is reduced, which directly leads to the increasing severity of the disease. For example, streptococcus and gill mold infection in tilapia, bacterial infection in river group, and Edwardsiella in yellow catfish occur frequently, causing serious economic lo...

Examples

Embodiment 1

[0035] Example 1: Take 10 parts of Poria cocos and weigh 20 parts of Ligustrum lucidum.

[0036] 1) Preliminary crushing: take 20 parts of Ligustrum lucidum and 10 parts of Poria cocos respectively, pass through a 200-mesh sieve after crushing respectively, and obtain Ligustrum lucidum powder and Poria cocos powder respectively;

[0037] 2) Water extraction: take Ligustrum lucidum powder, add 10 times its total weight in distilled water and soak for 0.5h, then bathe in 100°C water for 1.5h, and pass through a 300 mesh sieve to obtain the filtrate and filter residue of the first step of water extraction respectively. The filtrate is standby; add the same volume of distilled water to the filter residue of the first step, 100 ℃ water bath 1.5h, pass 300 mesh sieves, obtain the filtrate and filter residue of the second step water extraction respectively, the filtrate of the second step is standby; to the second step Add the same volume of distilled water to the filter residue, 100...

Embodiment 2

[0041] Example 2: Take 10 parts of Poria cocos and Ligustrum lucidum respectively.

[0042] 1) Preliminary crushing: take 10 parts of Ligustrum lucidum and 10 parts of Poria cocos respectively, and pass through a 200-mesh sieve after crushing respectively to obtain Ligustrum lucidum powder and Poria cocos powder respectively;

[0043] 2) Water extraction: take Ligustrum lucidum powder, add 10 times its total weight in distilled water and soak for 0.5h, then bathe in 100°C water for 1.5h, and pass through a 300 mesh sieve to obtain the filtrate and filter residue of the first step of water extraction respectively. The filtrate is standby; add the same volume of distilled water to the filter residue of the first step, 100 ℃ water bath 1.5h, pass 300 mesh sieves, obtain the filtrate and filter residue of the second step water extraction respectively, the filtrate of the second step is standby; to the second step Add the same volume of distilled water to the filter residue, 100 ° ...

Embodiment 3

[0047] Embodiment 3: 15 parts of Poria cocos and Ligustrum lucidum were weighed respectively.

[0048] 1) Preliminary crushing: take 15 parts of Ligustrum lucidum and 15 parts of Poria cocos respectively, pass through a 200-mesh sieve after crushing respectively, and obtain Ligustrum lucidum powder and Poria cocos powder respectively;

[0049] 2) Water extraction: take Ligustrum lucidum powder, add 10 times its total weight in distilled water and soak for 0.5h, then bathe in 100°C water for 1.5h, and pass through a 300 mesh sieve to obtain the filtrate and filter residue of the first step of water extraction respectively. The filtrate is standby; add the same volume of distilled water to the filter residue of the first step, 100 ℃ water bath 1.5h, pass 300 mesh sieves, obtain the filtrate and filter residue of the second step water extraction respectively, the filtrate of the second step is standby; to the second step Add the same volume of distilled water to the filter residu...

technical field

[0001] The invention relates to an immune enhancer for aquaculture, which belongs to a compound preparation of Chinese herbal medicine, and specifically refers to a compound immune enhancer of Chinese herbal medicine, a preparation method and its application as an immune enhancer in aquatic fish farming. Background technique

[0002] In recent years, with the rapid development of my country's aquaculture industry. With the high yield and low cost of today's high-density and intensive aquaculture fish farming, excessive aquaculture density will directly bring a huge load to the water environment, and the fish body will also be stressed, reducing the resistance of fish to diseases , the physiological function is reduced, which directly leads to the increasing severity of the disease. For example, streptococcus and gill mold infection in tilapia, bacterial infection in river group, and Edwardsiella in yellow catfish occur frequently, causing serious economic lo...

Examples

Embodiment 1

[0035] Example 1: Take 10 parts of Poria cocos and weigh 20 parts of Ligustrum lucidum.

[0036] 1) Preliminary crushing: take 20 parts of Ligustrum lucidum and 10 parts of Poria cocos respectively, pass through a 200-mesh sieve after crushing respectively, and obtain Ligustrum lucidum powder and Poria cocos powder respectively;

[0037] 2) Water extraction: take Ligustrum lucidum powder, add 10 times its total weight in distilled water and soak for 0.5h, then bathe in 100°C water for 1.5h, and pass through a 300 mesh sieve to obtain the filtrate and filter residue of the first step of water extraction respectively. The filtrate is standby; add the same volume of distilled water to the filter residue of the first step, 100 ℃ water bath 1.5h, pass 300 mesh sieves, obtain the filtrate and filter residue of the second step water extraction respectively, the filtrate of the second step is standby; to the second step Add the same volume of distilled water to the filter residue, 100...

Embodiment 2

[0041] Example 2: Take 10 parts of Poria cocos and Ligustrum lucidum respectively.

[0042] 1) Preliminary crushing: take 10 parts of Ligustrum lucidum and 10 parts of Poria cocos respectively, and pass through a 200-mesh sieve after crushing respectively to obtain Ligustrum lucidum powder and Poria cocos powder respectively;

[0043] 2) Water extraction: take Ligustrum lucidum powder, add 10 times its total weight in distilled water and soak for 0.5h, then bathe in 100°C water for 1.5h, and pass through a 300 mesh sieve to obtain the filtrate and filter residue of the first step of water extraction respectively. The filtrate is standby; add the same volume of distilled water to the filter residue of the first step, 100 ℃ water bath 1.5h, pass 300 mesh sieves, obtain the filtrate and filter residue of the second step water extraction respectively, the filtrate of the second step is standby; to the second step Add the same volume of distilled water to the filter residue, 100 ° ...

Embodiment 3

[0047] Embodiment 3: 15 parts of Poria cocos and Ligustrum lucidum were weighed respectively.

[0048] 1) Preliminary crushing: take 15 parts of Ligustrum lucidum and 15 parts of Poria cocos respectively, pass through a 200-mesh sieve after crushing respectively, and obtain Ligustrum lucidum powder and Poria cocos powder respectively;

[0049] 2) Water extraction: take Ligustrum lucidum powder, add 10 times its total weight in distilled water and soak for 0.5h, then bathe in 100°C water for 1.5h, and pass through a 300 mesh sieve to obtain the filtrate and filter residue of the first step of water extraction respectively. The filtrate is standby; add the same volume of distilled water to the filter residue of the first step, 100 ℃ water bath 1.5h, pass 300 mesh sieves, obtain the filtrate and filter residue of the second step water extraction respectively, the filtrate of the second step is standby; to the second step Add the same volume of distilled water to the filter residu...

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