A device suitable for rapid cell culture

Abstract

The present invention discloses a device suitable for rapid cell culture, comprising a cell culture box, an ultraviolet lamp is installed on the inner wall of the cell culture box, a partition is fixedly installed inside the cell culture box, a stepped through hole is provided on the partition, a support plate is fixedly connected inside the through hole, a slot is provided on the support plate, a culture dish is arranged inside the slot, the stability of the culture dish can be increased and the culture dish can be prevented from shaking on the support plate, a plurality of groups of air inlet holes are provided on the support plate, and a heating coil is installed on the inner bottom of the cell culture box. The present invention improves the temperature control effect of the cell culture box, so that the temperature inside the cell culture box fluctuates within a small and suitable range, and the temperature can be increased promptly and quickly, and adjusted to a temperature suitable for cell growth, thereby improving the growth rate of the cells to a certain extent and achieving rapid cell culture.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a device suitable for rapid cell culture. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Whether for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an indispensable process, and cell culture itself is the large-scale cloning of cells. Cell culture technology can transform a cell into a simple single cell or a multi-cell with very little differentiation through large-scale culture. This is an indispensable part of cloning technology, and cell culture itself is the cloning of cells; cell culture is divided into animal cell culture, plant cell culture and microbial cell culture, and the cell culture environment needs to be sterile, with a suitable temperature, suitable osmotic pressure and suitable pH value.

[0003] The temperature control effect of the cell culture device in the prior art is not good. The temperature variation range inside the incubator is large, and the temperature cannot be replenished in time and adjusted to a suitable temperature, which reduces the growth rate of cells. Therefore, we need to propose a device suitable for rapid cell culture. Summary of the invention

[0004] The object of the present invention is to provide a device suitable for rapid cell culture, which improves the temperature control effect of a cell culture box, allows the temperature inside the box to fluctuate within a smaller and appropriate range, and is able to promptly and quickly increase the temperature and adjust it to a temperature suitable for cell growth, thereby increasing the cell growth rate to a certain extent and achieving rapid cell culture to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device suitable for rapid cell culture, comprising a cell culture box, an ultraviolet lamp is installed on the inner wall of the cell culture box, a partition is fixedly installed inside the cell culture box, a stepped through hole is provided on the partition, a support plate is fixedly connected inside the through hole, a slot is provided on the support plate, a culture dish is arranged inside the slot, the stability of the culture dish can be increased and the culture dish can be prevented from shaking on the support plate, a plurality of groups of air inlet holes are provided on the support plate, a heating coil is installed on the inner bottom of the cell culture box, and when the temperature inside the cell culture box is lower than the set temperature value, the heating coil can increase the temperature inside the cell culture box;

[0006] A second drain pipe is arranged inside the cell culture box, a nutrient solution tank is arranged at the upper end of the cell culture box, a first drain pipe is connected to the discharge port at the bottom of the nutrient solution tank, a solenoid valve is installed on the first drain pipe, the end of the first drain pipe is connected to the second drain pipe, the second drain pipe is located at the upper end of the culture dish, and it is convenient to add nutrient solution to the culture dish, and a fill light is movably arranged at the upper end of the interior of the cell culture box, the fill light is located at the upper end of the culture dish, and the fill light plays a role of illumination to promote rapid growth of cells.

[0007] Preferably, the cell culture box is fixedly connected with support columns, the support columns are arranged in four groups in an array, and the nutrient solution tank is fixedly mounted on the four groups of support columns.

[0008] Preferably, a temperature control switch is fixedly installed on the side of the cell culture box, and the temperature control switch and the heating coil are electrically connected through a wire. The temperature control switch controls the heating of the heating coil, and the heating temperature of the heating coil does not exceed forty degrees to prevent damage to the cells.

[0009] Preferably, a trachea is fixedly installed on the side of the cell culture box, the trachea is configured as an L-shaped trachea, an air pump is installed on the trachea, one end of the trachea is located at the inner side of the cell culture box, and the other end of the trachea is located at the inner bottom of the cell culture box.

[0010] Preferably, a trachea is fixedly installed on the side of the cell culture box, the trachea is configured as an L-shaped trachea, an air pump is installed on the trachea, one end of the trachea is located at the inner side of the cell culture box, and the other end of the trachea is located at the inner bottom of the cell culture box.

[0011] Preferably, a temperature sensor is fixedly mounted on the partition to facilitate monitoring of the temperature inside the cell culture box, and two sets of connecting frames are fixedly mounted on the support plate, wherein the connecting frames are configured as U-shaped connecting frames to facilitate removing the support plate from the through hole.

[0012] Preferably, an electric push rod is fixedly installed on the inner top of the cell culture box, one end of the piston rod of the electric push rod is fixedly connected to a fixing frame, the fixing frame is configured as a square fixing frame, two groups of fixing rods are installed inside the fixing frame, the two groups of fixing rods are vertically arranged, and the two groups of fixing rods divide the interior of the fixing frame into four cavities of equal size, and the fill light is fixedly installed inside the cavity.

[0013] Preferably, the fill light is configured as a ring-shaped fill light, the nutrient solution dripping from the second drain pipe passes through the inner circle of the fill light, and the outer wall of the fill light is connected to the inner wall of the cavity.

[0014] Preferably, the second drain pipe is configured as a cross-shaped drain pipe, and four groups of drain ports are provided at the lower end of the second drain pipe. The drain ports are configured as truncated cone-shaped drain ports, and the drain ports are aligned with the culture dish. The nutrient solution in the nutrient solution tank drips into the culture dish from the liquid preparation port.

[0015] Preferably, a door is rotatably mounted on one side of the cell culture box, and an observation window is provided on the door to facilitate observation of the culture conditions of the cells in the cell culture box.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] Through the design of the electric push rod, the fixing frame, the fill light, the heating coil and the air pipe, the fill light is turned on to supplement the light for the cells to promote their growth. During the culture process, if the temperature sensor detects that the temperature inside the cell culture box is slightly lower than the set temperature, the electric push rod drives the fixing frame to move downward, and the fixing frame drives the fill light to move downward close to the culture dish. The heat generated by the fill light when working can increase the temperature around the culture dish, so that the cells are in a suitable temperature environment;

[0018] When the temperature sensor detects that the temperature inside the cell culture box is much lower than the set temperature, the temperature control switch controls the heating coil to generate heat, thereby increasing the temperature inside the cell culture box. Then, the air pump on the air pipe is turned on, and the heated gas flows inside the air pipe and blows upward from the bottom of the cell culture box. The hot air blows from the air inlet to the upper end of the partition, sending heat to the surroundings of the culture dish, so that it is in a suitable temperature environment.

[0019] The present invention improves the temperature control effect of the cell culture box, makes the temperature inside it fluctuate within a smaller and appropriate range, can promptly and quickly increase the temperature, and adjust it to a temperature suitable for cell growth, thereby increasing the growth rate of cells to a certain extent and achieving rapid cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of the present invention;

[0021] Figure 2 It is a schematic diagram of the internal structure of the present invention;

[0022] Figure 3 It is a schematic diagram of the structure of the fixing frame, the fixing rod and the fill light of the present invention;

[0023] Figure 4 It is a schematic diagram of the structure of the partition, temperature sensor, stepped through hole and slot of the present invention;

[0024] Figure 5 It is a schematic diagram of the structure of the support plate, the mounting frame, the air inlet hole and the card slot of the present invention;

[0025] Figure 6 It is a schematic structural diagram of the second liquid discharge pipe of the present invention.

[0026] In the figure: 1. Cell culture box; 2. Support column; 3. Nutrient solution box; 4. Box door; 5. Observation window; 6. Temperature control switch; 7. First drain pipe; 8. Solenoid valve; 9. Second drain pipe; 10. Electric push rod; 11. Fixed frame; 12. Partition; 13. Support plate; 14. Culture dish; 15. Ultraviolet lamp; 16. Temperature sensor; 17. Heating coil; 18. Air pipe; 19. Fixed rod; 20. Fill light; 21. Through hole; 22. Slot; 23. Plug column; 24. Connecting frame; 25. Air inlet; 26. Card slot. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The same or similar components are indicated by the same reference numerals in different drawings. In addition, please understand that terms such as "first", "second", "third", "upper", "lower", "front", "rear", "inner", "outer", "end", "portion", "segment", "width", "thickness", "region" and the like in the text are only for the convenience of readers to refer to the structures in the drawings and are only used to help describe the present invention, and are not limitations of the present invention.

[0029] See also Figure 1-6 The present invention provides a technical solution: a device suitable for rapid cell culture, comprising a cell culture box 1, a box door 4 is rotatably installed on one side of the cell culture box 1, and the box door 4 is in a sealed state when closed to prevent external dust and bacteria from entering, and an observation window 5 is arranged on the box door 4 to facilitate observation of the culture status of cells in the cell culture box 1, and an ultraviolet lamp 15 is installed on the inner wall of the cell culture box 1 to facilitate killing bacteria and viruses in the cell culture box 1, and a partition 12 is fixedly installed inside the cell culture box 1, and a stepped through hole 21 is opened on the partition 12, and a support plate 13 is fixedly connected to the inside of the through hole 21, and a plug column 23 is fixedly connected to the lower end of the support plate 13, and the plug column 23 is arranged in four groups in an array, and a slot 22 is opened at the inner bottom of the through hole 21, and the slot 22 and the plug column 23 are arranged correspondingly, and the plug column 23 is inserted into the inside of the slot 22 to prevent the support plate 13 from shaking left and right;

[0030] Four groups of slots 26 are provided on the support plate 13, and a culture dish 14 is arranged inside the slots 26 to increase the stability of the culture dish 14 and prevent it from shaking on the support plate 13. Multiple groups of air inlet holes 25 are provided on the support plate 13, and a temperature sensor 16 is fixedly installed on the partition plate 12 to facilitate monitoring the temperature inside the cell culture box 1. Two groups of connecting frames 24 are fixedly installed on the support plate 13, and the connecting frames 24 are set as U-shaped connecting frames to facilitate taking the support plate 13 out of the through hole 21;

[0031] A heating coil 17 is installed at the inner bottom of the cell culture box 1, and a temperature control switch 6 is fixedly installed on the side of the cell culture box 1. The temperature control switch 6 and the heating coil 17 are electrically connected through a wire. The temperature control switch 6 controls the heating coil 17 to generate heat, and the heating temperature of the heating coil 17 does not exceed 40 degrees to prevent damage to the cells. When the temperature inside the cell culture box 1 is lower than the set temperature value, the heating coil 17 can increase the temperature inside the cell culture box 1 so that the cell culture process is in a suitable temperature environment. An air pipe 18 is fixedly installed on the side of the cell culture box 1. The air pipe 18 is set to be an L-shaped air pipe. An air pump is installed on the air pipe 18. One end of the air pipe 18 is located at the inner side of the cell culture box 1, and the other end of the air pipe 18 is located at the inner bottom of the cell culture box 1. Specifically, one end of the air pipe 18 located at the inner side is the air inlet end, and the other end is the air outlet end, which can blow the gas heated by the heating coil 17 upward;

[0032] The cell culture box 1 is provided with a second drain pipe 9, and a nutrient solution tank 3 is provided at the upper end of the cell culture box 1. The nutrient solution tank 3 is provided with nutrient solution. The cell culture box 1 is fixedly connected with support columns 2. The support columns 2 are arranged in four groups in an array. The nutrient solution tank 3 is fixedly installed on the four groups of support columns 2. The discharge port at the bottom is connected to a first drain pipe 7, on which a solenoid valve 8 is installed. The end of the first drain pipe 7 passes through the cell culture box 1 and is connected to the second drain pipe 9. The second drain pipe 9 is located at the upper end of the culture dish 14, which is convenient for adding nutrient solution to the culture dish 14. The second drain pipe 9 is configured as a cross-shaped drain pipe, and the lower end of the second drain pipe 9 is provided with four groups of drain ports, which are configured as truncated cone-shaped drain ports. The drain ports and the culture dish 14 are aligned. The nutrient solution in the nutrient solution tank 3 drips from the liquid dispensing port into the culture dish 14, and the nutrient solution can be automatically added. The nutrient solution is set to drip one drop every two hours, and the dripping rate of the nutrient solution can be adjusted by the solenoid valve 8.

[0033] A fill light 20 is movably arranged at the upper end of the interior of the cell culture box 1. An electric push rod 10 is fixedly installed at the inner top of the cell culture box 1. One end of the piston rod of the electric push rod 10 is fixedly connected to a fixing frame 11. The electric push rod 10 drives the fixing frame 11 to move up and down. The fixing frame 11 is set as a square fixing frame. Two groups of fixing rods 19 are installed inside the fixing frame 11. The two groups of fixing rods 19 are vertically arranged, and the two groups of fixing rods 19 divide the interior of the fixing frame 11 into four cavities of equal size. The fill light 20 is fixedly installed inside the cavity. The fill light 20 is set as a ring-shaped fill light. The nutrient solution dripping from the second drain pipe 9 is discharged from the fill light 20. The inner circle passes through and drips into the culture dish 14. The outer wall of the fill light 20 is connected to the inner wall of the cavity. The fill light 20 is located at the upper end of the culture dish 14. Specifically, the fill light 20 is located between the second drain pipe 9 and the culture dish 14. The fill light 20 illuminates the cells in the culture dish 14 and promotes rapid growth of the cells.

[0034] When using the device, first turn on the ultraviolet lamp 15 to sterilize and disinfect the inside of the cell culture box 1. After the treatment is completed, open the box door 4 and put the support plate 13 into the inside of the cell culture box 1. Specifically, the support plate 13 is placed inside the through hole 21, and the plug 23 at the lower end of the support plate 13 is plugged into the inside of the slot 22 to prevent the support plate 13 from shaking. Then, the culture dish 14 with cells cultured is placed into the inside of the card slot 26 for cell culture. Close the box door 4 and turn on the supplementary light 20 to supplement the light for the cells to promote their growth. During the culture process, if the temperature sensor 16 detects that the temperature inside the cell culture box 1 is slightly lower than the set temperature, the electric push rod 10 can be started to drive the fixing frame 11 to move downward. The fixing frame 11 drives the supplementary light 20 to move downward close to the culture dish 14. The heat generated by the supplementary light 20 during operation can increase the temperature around the culture dish 14, so that the cells are in a suitable temperature environment.

[0035] When the temperature sensor 16 detects that the temperature inside the cell culture box 1 is much lower than the set temperature, the temperature control switch 6 is turned on to control the heating coil 17 to generate heat, thereby increasing the temperature inside the cell culture box 1. Then, the air pump on the air pipe 18 is turned on, and the heated gas flows inside the air pipe 18 and then blows upward from the bottom of the cell culture box 1. The hot air blows from the air inlet 25 to the upper end of the partition 12, thereby delivering heat to the surroundings of the culture dish 14, thereby placing the culture dish 14 in a suitable temperature environment.

[0036] During the cell culture process, nutrient solution needs to be added to the culture dish 14 , the solenoid valve 8 is opened, the nutrient solution in the nutrient solution tank 3 flows into the first drain pipe 7 , and is discharged from the drain port on the second drain pipe 9 , and then the nutrient solution drips into the culture dish 14 .

[0037] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device suitable for rapid cell culture, comprising a cell culture chamber (1), characterized in that: An ultraviolet lamp (15) is installed on the inner wall of the cell culture box (1); a partition (12) is fixedly installed inside the cell culture box (1); a stepped through hole (21) is provided on the partition (12); a support plate (13) is fixedly connected inside the through hole (21); a slot (26) is provided on the support plate (13); a culture dish (14) is arranged inside the slot (26), so as to increase the stability of the culture dish (14) and prevent it from shaking on the support plate (13). The support plate (13) is provided with a plurality of groups of air inlet holes (25), and a heating coil (17) is installed at the inner bottom of the cell culture box (1). When the temperature inside the cell culture box (1) is lower than a set temperature value, the heating coil (17) can increase the temperature inside the cell culture box (1); A second drain pipe (9) is arranged inside the cell culture box (1); a nutrient solution tank (3) is arranged at the upper end of the cell culture box (1); a first drain pipe (7) is connected to the discharge port at the bottom of the nutrient solution tank (3); a solenoid valve (8) is installed on the first drain pipe (7); an end of the first drain pipe (7) is connected to the second drain pipe (9); the second drain pipe (9) is located at the upper end of the culture dish (14) to facilitate the addition of nutrient solution to the culture dish (14); A fill light (20) is movably provided at the upper end of the interior of the cell culture box (1); the fill light (20) is located at the upper end of the culture dish (14); the fill light (20) plays a role of illumination, thereby promoting rapid growth of cells; A temperature control switch (6) is fixedly mounted on the side of the cell culture box (1), and the temperature control switch (6) and the heating coil (17) are electrically connected via a wire. The temperature control switch (6) controls the heating coil (17) to generate heat, and the heating temperature of the heating coil (17) does not exceed 40 degrees to prevent damage to the cells. An air tube (18) is fixedly mounted on the side of the cell culture box (1), the air tube (18) being configured as an L-shaped air tube, an air pump being mounted on the air tube (18), one end of the air tube (18) being located on the inner side of the cell culture box (1), and the other end of the air tube (18) being located on the inner bottom of the cell culture box (1); An electric push rod (10) is fixedly installed on the top of the cell culture box (1), one end of the piston rod of the electric push rod (10) is fixedly connected to a fixing frame (11), the fixing frame (11) is arranged as a square fixing frame, two groups of fixing rods (19) are installed inside the fixing frame (11), the two groups of fixing rods (19) are arranged vertically, and the two groups of fixing rods (19) divide the inside of the fixing frame (11) into four cavities of equal size, and the fill light (20) is fixedly installed inside the cavity; The fill light (20) is configured as a ring-shaped fill light, the nutrient solution dripping from the second liquid discharge pipe (9) passes through the inner ring of the fill light (20), and the outer wall of the fill light (20) is connected to the inner wall of the cavity.

2. A device for rapid cell culture according to claim 1, characterized in that: The cell culture box (1) is fixedly connected to support columns (2), the support columns (2) are arranged in four groups in an array, and the nutrient solution tank (3) is fixedly mounted on the four groups of support columns (2).

3. A device for rapid cell culture according to claim 1, characterized in that: The lower end of the support plate (13) is fixedly connected with an insertion column (23), and the insertion columns (23) are arranged in four groups in an array. The inner bottom of the through hole (21) is provided with a slot (22), and the slot (22) and the insertion column (23) are arranged correspondingly, and the insertion column (23) is inserted into the inside of the slot (22).

4. A device for rapid cell culture according to claim 1, characterized in that: A temperature sensor (16) is fixedly mounted on the partition (12) to facilitate monitoring of the temperature inside the cell culture chamber (1). Two groups of connecting frames (24) are fixedly mounted on the support plate (13). The connecting frames (24) are configured as U-shaped connecting frames to facilitate removing the support plate (13) from the through hole (21).

5. The device for rapid cell culture according to claim 1, characterized in that: The second liquid discharge pipe (9) is configured as a cross-shaped liquid discharge pipe. The lower end of the second liquid discharge pipe (9) is provided with four groups of liquid discharge ports. The liquid discharge ports are configured as truncated cone-shaped liquid discharge ports. The liquid discharge ports and the culture dish (14) are aligned with each other. The nutrient solution in the nutrient solution tank (3) drips from the liquid distribution port into the culture dish (14).

6. A device for rapid cell culture according to claim 1, characterized in that: A door (4) is rotatably mounted on one side of the cell culture box (1), and an observation window (5) is provided on the door (4) to facilitate observation of the culture conditions of the cells in the cell culture box (1).

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