A detection device and a detection method thereof

Abstract

The embodiment of the present invention discloses a detection device and a detection method thereof, wherein the detection device comprises: a detection array, comprising m*n detection units; a row scanning circuit and m scanning signal lines, wherein one scanning signal line is electrically connected to the control end of a row of detection units, and the row scanning circuit is used to provide scanning signals to the m scanning signal lines in a time-sharing manner; a column detection circuit, n first signal lines and n second signal lines, wherein one first signal line is electrically connected to the first end of a column of detection units, and one second signal line is electrically connected to the second end of a column of detection units, and the column detection circuit is used to provide a first detection signal to the n first signal lines, and obtain the second detection signals of a row of multiple detection units simultaneously through multiple second signal lines. In the embodiment of the present invention, the detection device can realize multi-site batch detection and improve detection efficiency.

Description

Technical Field

[0001] The embodiments of the present invention relate to the technical field of sample detection, and in particular to a detection device and a detection method thereof. Background Art

[0002] Biochemical indicator detection devices have broad application prospects in the fields of life science research, biomedical engineering, health care, food processing, environmental monitoring, etc.

[0003] Existing biochemical index detection devices mostly use ion-sensitive field effect transistors. The gate surface of the ion-sensitive field effect transistor has an ion-sensitive membrane, which can directly contact and react with ions in the solution to be tested, thereby detecting multiple biochemical indicators of the solution to be tested.

[0004] However, the detection efficiency of existing detection devices is too low. Summary of the invention

[0005] The embodiment of the present invention provides a detection device and a detection method thereof to solve the problem of low efficiency of existing sample detection.

[0006] An embodiment of the present invention provides a detection device, including:

[0007] A detection array, comprising m*n detection units;

[0008] A row scanning circuit and m scanning signal lines, wherein one scanning signal line is electrically connected to the control end of a row of detection units, and the row scanning circuit is used to provide scanning signals to the m scanning signal lines in a time-sharing manner;

[0009] A column detection circuit, n first signal lines and n second signal lines, one first signal line is electrically connected to the first end of a column of the detection units, one second signal line is electrically connected to the second end of a column of the detection units, the column detection circuit is used to provide a first detection signal to the n first signal lines, and to simultaneously obtain second detection signals of a row of multiple detection units through multiple second signal lines.

[0010] The embodiment of the present invention further provides a detection method of a detection device. The detection method of the detection device as described above comprises: S frame data acquisition pages, S is greater than or equal to 1;

[0011] In one frame of the data acquisition page, the row scanning circuit provides scanning signals to the m scanning signal lines row by row, and the column detection circuit provides first detection signals to the n first signal lines; the column detection circuit simultaneously obtains second detection signals of a plurality of detection units in a row through a plurality of the second signal lines;

[0012] The detection method also includes a detection phase, which includes at least one frame of the data acquisition page. In the detection phase, the threshold drift amount of the detection unit is calculated according to the first detection signal, the second detection signal and the preset threshold voltage of the detection unit.

[0013] In an embodiment of the present invention, the row scanning circuit controls the time-sharing scanning of m rows of detection units through m scanning signal lines. In the detection stage of a row of detection units, the row scanning circuit provides an effective scanning signal to the row of detection units through the scanning signal lines to make them turn on at the same time. The column detection circuit provides a first detection signal to the first ends of multiple detection units in a row through multiple first signal lines among n lines, and then obtains the second detection signal of the second ends of multiple detection units in a row through multiple second signal lines. In an embodiment of the present invention, the detection device can determine the change of the electrical parameters of the detection unit by testing the scanning signal, the first detection signal and the second detection signal of the detection unit. According to the change of the electrical parameters of the detection unit, the sample at the site where the detection unit is located can be detected, thereby realizing multi-site batch detection of samples such as liquids and improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the prior art descriptions. Obviously, although the drawings described below are some specific embodiments of the present invention, for those skilled in the art, the basic concepts of the device structure, driving method and manufacturing method disclosed and suggested by the various embodiments of the present invention can be expanded and extended to other structures and drawings, and there is no doubt that these should be within the scope of the claims of the present invention.

[0015] Figure 1 is a schematic diagram of a detection device provided by an embodiment of the present invention;

[0016] Figure 2 is a schematic diagram of a detection unit;

[0017] Figure 3 is a schematic diagram of another detection device provided by an embodiment of the present invention;

[0018] Figure 4 A schematic diagram of a detection signal provided by an embodiment of the present invention;

[0019] Figure 5 is a schematic diagram of another detection device provided by an embodiment of the present invention;

[0020] Figure 6 Another detection signal schematic diagram provided by an embodiment of the present invention;

[0021] Figure 7is a schematic diagram of another detection device provided by an embodiment of the present invention;

[0022] Figure 8 is a schematic diagram of another detection device provided by an embodiment of the present invention;

[0023] Fig. 9 is a schematic diagram of another detection device provided by an embodiment of the present invention;

[0024] Fig.10 is a schematic diagram of a detection method of a detection device provided by an embodiment of the present invention;

[0025] Fig.11 is a schematic diagram of another detection method of a detection device provided by an embodiment of the present invention;

[0026] Fig.12 It is a curve diagram of the detection unit in the self-calibration stage;

[0027] Fig.13 It is a curve diagram of the detection unit in the detection stage;

[0028] Fig.14 It is a schematic diagram of a detection method of another detection device provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described through implementation methods with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the basic concepts disclosed and suggested by the embodiments of the present invention, all other embodiments obtained by those skilled in the art are within the scope of protection of the present invention.

[0030] refer to Figure 1 As shown in FIG. 1 , a schematic diagram of a detection device provided by an embodiment of the present invention is shown. Figure 1 As shown, the detection device provided in this embodiment includes: a detection array 10, including m*n detection units 11; a row scanning circuit 20 and m scanning signal lines 21, one scanning signal line 21 is electrically connected to the control end c of a row of detection units 11, and the row scanning circuit 20 is used to provide scanning signals to the m scanning signal lines 21 in a time-sharing manner; a column detection circuit 30, n first signal lines 31 and n second signal lines 32, one first signal line 31 is electrically connected to the first end a of a column of detection units 11, and one second signal line 32 is electrically connected to the second end b of a column of detection units 11, and the column detection circuit 30 is used to provide a first detection signal to the n first signal lines 31, and simultaneously obtain the second detection signals of a row of multiple detection units 11 through multiple second signal lines 32.

[0031] In this embodiment, the detection device can be immersed in liquid, so the detection array 10 can be used to perform batch detection of different sites in the liquid. Alternatively, multiple droplets can be dripped onto the detection device, so the detection array 10 can be used to perform batch detection of different droplets. It can be understood that the detection device can be used in various sample detections to achieve multi-site detection.

[0032] The detection device includes m*n detection units 11, m and n are both positive integers, and m*n is greater than or equal to 2. The detection unit 11 includes a control terminal c, a first terminal a, and a second terminal b. The scanning signal line 21 is electrically connected to the control terminal c of the detection unit 11. If the scanning signal line 21 outputs a valid scanning signal, the scanning signal line 21 controls the first terminal a and the second terminal b of the detection unit 11 to be turned on through the control terminal c of the detection unit 11. If the scanning signal line 21 outputs an invalid scanning signal, the scanning signal line 21 controls the first terminal a and the second terminal b of the detection unit 11 to be turned off through the control terminal c of the detection unit 11. A scanning signal line 21 is electrically connected to the control terminal c of a row of detection units 11, and is used to control each detection unit 11 in the row to be turned on or off at the same time. The row scanning circuit 20 is used to provide scanning signals to the m scanning signal lines 21 in a time-sharing manner. The row scanning circuit 20 can optionally provide scanning signals to the m scanning signal lines 21 row by row, for example, scanning row by row from top to bottom in the diagram. It can be understood that the scanning mode of the row scanning circuit can be either row-by-row scanning or interlaced scanning, without specific limitation.

[0033] The first signal line 31 is electrically connected to the first end a of each detection unit 11 in a column of detection units 11, and the second signal line 32 is electrically connected to the second end b of each detection unit 11 in a column of detection units 11. The column detection circuit 30 is used to provide a first detection signal to n first signal lines 31, and simultaneously obtain a second detection signal of a row of multiple detection units 11 through a plurality of second signal lines 32. Obviously, when the scanning signal line 21 controls the first end a and the second end b of the detection unit 11 to be turned on, the column detection circuit 30 provides the first detection signal to the first end a of the detection unit 11 through the first signal line 31, and the column detection circuit 30 obtains the second detection signal provided by the second end b of the detection unit 11 through the second signal line 32.

[0034] It can be understood that the sample such as liquid at the location of the detection unit 11 affects the electrical parameters of the detection unit 11. The detection unit 11 may optionally include an ion-sensitive field effect transistor, and the electrical parameters of the detection unit 11 include at least the threshold voltage of the transistor. The liquid at the location of the detection unit 11 affects its threshold voltage. When the detection unit 11 receives the same first detection signal, as the scanning signal changes, its output terminal generates a second detection signal related to the scanning signal. The column detection circuit 30 is used to simultaneously provide a first detection signal to the first end a of multiple detection units 11 in a row through multiple first signal lines 31 among n lines, and then obtain a second detection signal from the second end b of multiple detection units 11 in a row through multiple second signal lines 32 among n lines. According to the change of the second detection signal caused by the change of the electrical parameters of the detection unit 11, the threshold voltage drift of the inspection unit 11 can be calculated, and then according to the threshold voltage drift of the detection unit 11, the sample at the location of the detection unit 11 can be detected, so as to realize multi-site batch detection of samples such as liquids and improve the detection efficiency.

[0035] For example, n=100, the column detection circuit 30 simultaneously provides the first detection signal to the first end a of the first 50 detection units 11 in a row through the 1st to 50th first signal lines 31, and then the column detection circuit 30 obtains the second detection signal from the second end b of the first 50 detection units 11 in a row through the 1st to 50th second signal lines 32, thereby realizing batch liquid detection at the sites where the first 50 detection units 11 in the row are located. Alternatively, n=100, the column detection circuit 30 simultaneously provides the first detection signal to the first end a of each detection unit 11 in a row through 100 first signal lines 31, and then the column detection circuit 30 obtains the second detection signal from the second end b of each detection unit 11 in a row through 100 second signal lines 32, thereby realizing batch liquid detection at the sites where the 100 detection units 11 in the row are located. Alternatively, n=100, the column detection circuit 30 provides a first detection signal to the first end a of the 79th detection unit 11 in a row through the 79th first signal line 31, and then the column detection circuit 30 obtains its second detection signal through the second signal line 32 electrically connected to the detection unit 11, thereby realizing the individual liquid detection at the position where the 79th detection unit 11 in the row is located. Obviously, there are many detection methods for the detection device, and any detection unit can be detected independently, multiple detection units in a row can be detected in batches, and multiple detection units in multiple rows can be detected in batches.

[0036] It can be understood that the column detection circuit may include multiple modules, for example, a first module, which can be used to provide a first detection signal to n first signal lines; a second module, which can be used to obtain a second detection signal of each detection unit through n second signal lines; and a processing module, which is used to perform signal processing according to the first detection signal, the second detection signal and the scanning signal to obtain the required sample parameters. It should be noted that Figure 1 The column detection circuit 30 shown is located at one side of the detection array 10 for illustration only. Optionally, the first module, the second module and the processing module included in the column detection circuit can be located at one side of the detection array 10, or at different sides of the detection array 10, which can be specifically arranged according to the actual space of the detection device.

[0037] Specifically, the row scanning circuit 20 provides an effective scanning signal to the control terminal c of the first row detection unit 11 through the first scanning signal line 21, so that the n detection units 11 in the first row are turned on at the same time, and the detection units 11 in other rows remain turned off; the column detection circuit 30 provides a first detection signal to the first terminal a of the multiple detection units 11 in the first row through multiple first signal lines 31 among n, and then the column detection circuit 30 can obtain the second detection signal provided by the second terminal b of the multiple detection units 11 in the first row that are turned on through multiple second signal lines 32 among n. The column detection circuit 30 can obtain the change of the electrical parameters of any detection unit 11 in the first row according to the scanning signal received, the first detection signal and the output second detection signal of any detection unit 11 in the first row, so as to realize batch detection of samples such as liquids at multiple sites. The electrical parameters of the optional detection unit 11 include a threshold voltage. Specifically, the input end of the detection unit 11 receives a constant voltage signal, and the threshold voltage of the detection unit 11 changes due to the influence of the liquid. Then, as the scanning signal changes, the output end of the detection unit 11 will generate a second detection signal associated with the scanning signal. Based on this, the actual threshold voltage of the detection unit 11 can be determined according to the second detection signal, and the threshold voltage drift of the detection unit 11 can be calculated, and then the liquid parameters of the site can be calculated according to the threshold voltage drift.

[0038] Sequentially, the row scanning circuit 20 provides an effective scanning signal to the control terminal c of the second row detection unit 11 through the second scanning signal line 21, thereby realizing the electrical parameter detection of multiple detection units 11 in the second row; and so on, multiple rows of detection units 11 are detected, thereby realizing batch detection of samples such as liquids at multiple locations.

[0039] The optional detection unit 11 includes a dual-gate ion-sensitive field effect transistor; the dual-gate ion-sensitive field effect transistor includes a control end, an input end and an output end, the control end of the dual-gate ion-sensitive field effect transistor is electrically connected to the scanning signal line 21, the input end is electrically connected to the first signal line 31, and the output end is electrically connected to the second signal line 32.

[0040] For example, refer to Figure 2 As shown in FIG. , it is a schematic diagram of the detection unit. Figure 2 As shown, the detection unit 11 includes a dual-gate ion-sensitive field effect transistor (ISFET), which includes a substrate 111, a bottom gate 112, an IGZO gate 113 and a source and drain 114, and the surface of the transistor also has an ion-sensitive layer 115. The detection unit 11 is immersed in a solution to be tested, which is an electrolyte solution 116, and a grounded reference electrode 117 is also provided in the solution to be tested. The concentration of hydrogen ions (H+) at different sites in the solution to be tested may be different or change. In the detection process, the surface charge of the ion-sensitive layer 115 of the detection unit 11 changes according to the different concentrations of hydrogen ions (H+) in the solution to be tested at the site position, which will affect the IGZO gate 113 of the detection unit 11, so that the threshold voltage of the detection unit 11 changes. Therefore, the change in the threshold voltage of the detection unit 11 can be calculated based on the signal at the output end of the inspection unit 11, and the pH value of the solution at the site can be determined based on the change in the threshold voltage.

[0041] Specifically, in this embodiment, by applying a scanning signal and a first detection signal to the detection unit 11, and then detecting to obtain a second detection signal, the actual threshold voltage of the detection unit 11 can be obtained by analyzing and processing the second detection signal. The difference between the actual threshold voltage of the detection unit 11 and the initial threshold voltage is the threshold voltage change of the detection unit 11, and then the pH value of the solution at the location of the detection unit 11 is determined. By modifying different ion-sensitive layers on the surface of the detection unit 11, the corresponding ion concentration of the surface liquid can be obtained through a similar mechanism.

[0042] The first detection signal provided by the column detection circuit 30 to the first signal line 31 can be a constant DC signal V D .

[0043] In an embodiment of the present invention, a row scanning circuit controls the time-sharing scanning of m rows of detection units through m scanning signal lines. In the detection stage of a row of detection units, the row scanning circuit provides an effective scanning signal to the row of detection units through the scanning signal lines to make them turn on at the same time. The column detection circuit provides a first detection signal to the first ends of multiple detection units in a row through multiple first signal lines among n lines, and then obtains a second detection signal from the second ends of multiple detection units in a row through multiple second signal lines. In an embodiment of the present invention, the detection device applies a scanning signal and a first detection signal to the detection unit, and then obtains a second detection signal from the output end of the detection unit. The electrical parameter change of the detection unit can be determined by analyzing and processing the second detection signal. According to the electrical parameter change of the detection unit, the sample at the site where the detection unit is located can be detected, thereby realizing multi-site batch detection of samples such as liquids and improving detection efficiency.

[0044] refer to Figure 3 FIG. 1 is a schematic diagram of another detection device provided by an embodiment of the present invention. Figure 3 In the detection device shown, the column detection circuit 30 includes one first detection signal terminal Sa and n second detection signal terminals Sb; the first detection signal terminal Sa is electrically connected to n first signal lines 31, and is used to provide the first detection signal to the n first signal lines 31 at the same time; a second detection signal terminal Sb is electrically connected to a second signal line 32, and is used to collect the second detection signal of the second signal line 32.

[0045] In this embodiment, the column detection circuit 30 includes one first detection signal terminal Sa, and the one first detection signal terminal Sa simultaneously provides a first detection signal to n first signal lines 31. The column detection circuit 30 includes n second detection signal terminals Sb, and one second detection signal terminal Sb is electrically connected to one second signal line 32 correspondingly, and is used to collect the second detection signal of one second signal line 32.

[0046] In the detection stage of a row of detection units, the row scanning circuit 20 provides a valid scanning signal to the control terminal c of the row detection unit 11 through the scanning signal line 21, then the first terminal a and the second terminal b of any detection unit 11 in the row are turned on, and the other row detection units 11 remain turned off. The column detection circuit 30 provides a first detection signal to n first signal lines 31 through the first detection signal terminal Sa at the same time, and then the column detection circuit 30 can obtain the second detection signal provided by the second terminal b of each detection unit 11 in the turned-on row through n second signal lines 32. The column detection circuit 30 can calculate the threshold voltage change of any detection unit 11 in the row based on the scanning signal, the first detection signal and the output second detection signal received by the detection unit 11 in the row, so as to realize batch detection of samples such as liquids at multiple sites.

[0047] In the present embodiment, for the first detection signal, the column detection circuit 30 may be provided with at least one first detection signal terminal Sa, so as to provide the first detection signal to n first signal lines 31 at the same time. Obviously, by providing one first detection signal terminal Sa in the column detection circuit 30, the number of ports providing the first detection signal in the column detection circuit 30 can be significantly reduced, thereby reducing the cost of the column detection circuit 30.

[0048] refer to Figure 4 As shown, it is a schematic diagram of a detection signal provided by an embodiment of the present invention, such as Figure 4 The scanning signal provided by the optional row scanning circuit to the scanning signal line is a sawtooth wave signal.

[0049] refer to Figure 5 FIG. 1 is a schematic diagram of another detection device provided by an embodiment of the present invention, Figure 5 The optional detection unit 11 shown includes an N-type field effect transistor, and the N-type field effect transistor has a standard threshold voltage V greater than 0V. TH ; The sawtooth signal is from V GL Rising to V GH , where V GL Less than or equal to 0V, and V GH Greater than V TH .like Figure 5 The N-type field effect transistor shown, the first end a of the optional detection unit 11 is the drain of the N-type field effect transistor, the second end b of the detection unit 11 is the source of the N-type field effect transistor, and the control end c of the detection unit 11 is the gate of the N-type field effect transistor.

[0050] The first detection signal provided by the column detection circuit 30 to the first signal line 31 can be a constant DC signal V D The optional detection unit 11 includes an N-type field effect transistor, a constant DC signal V D Greater than the standard threshold voltage V of an N-type field effect transistor TH .

[0051] Combination Figure 4 and Figure 5 As shown, the scanning signal provided by the row scanning circuit 20 to the scanning signal line 21 is a sawtooth wave signal. The row scanning circuit 20 can optionally provide scanning signals to the m scanning signal lines 21 row by row. The scanning signals received by the m scanning signal lines 21 from top to bottom in sequence are marked as G1, G2, ..., Gm, all of which are sawtooth wave signals.

[0052] The detection unit 11 includes an N-type field effect transistor, and the N-type field effect transistor has a standard threshold voltage V greater than 0V. THThe standard threshold voltage mentioned here is the initial threshold voltage detected after the detection unit 11 is manufactured. After the detection unit 11 is immersed in liquid, the threshold voltage may change due to the influence of the liquid, and thus be different from the initial threshold voltage. The sawtooth wave signal is V GL When V GL Less than or equal to 0V, so the N-type field effect transistor is in the cut-off region, and its state is cut-off state; the sawtooth wave signal is V GH When V GH Greater than V TH , so the N-type field effect transistor is in the saturation region and its state is the on state. Based on this, it can be seen that when the sawtooth wave signal received by the control terminal c of the detection unit 11 changes from V GL Rising to V GH When the N-type field effect transistor enters the saturation region from the cut-off region, the column detection circuit 30 determines the second detection signal value corresponding to the detection unit 11 entering the saturation region from the cut-off region according to the second detection signal output by the detection unit 11, and the scanning signal voltage value corresponding to the second detection signal value is the actual threshold voltage of the detection unit 11.

[0053] In this embodiment, when the detection device is immersed in the solution to be tested, the surface charge of the ion-sensitive layer of the detection unit 11 changes according to the different hydrogen ion (H+) concentrations of the solution at the site position, which affects the threshold voltage of the detection unit 11. This causes the threshold voltage of the N-type field effect transistor in the detection unit 11 to switch from the off state to the on state to change, and the threshold voltage of the N-type field effect transistor may no longer be equal to the standard threshold voltage V TH .

[0054] Based on this, the actual threshold voltage of the detection unit 11 is calculated according to the second detection signal. It is known that the factor affecting the threshold voltage of the detection unit 11 is the hydrogen ion (H+) concentration of the solution at its site position. Then, according to the second signal line 32, the second detection signal of the threshold voltage change of the detection unit 11 is output. The second detection signal value when the detection unit 11 switches from the cut-off state to the on state can be obtained by analyzing and processing the second detection signal. The scanning signal voltage value corresponding to the second detection signal value is determined as the actual threshold voltage of the detection unit 11. Based on this, the threshold voltage drift can be calculated, and then the hydrogen ion (H+) concentration of the solution at its site position can be calculated according to the threshold voltage drift.

[0055] Combination Figure 4 and Figure 5 As shown, for example, in the detection phase of the first row detection unit 11, the row scanning circuit 20 provides a scanning signal G1 to the control terminal c of each detection unit 11 in the first row through the first scanning signal line 21, and G1 is a voltage from V GL Rising to V GHThe column detection circuit 30 provides the first detection signal V to the n first signal lines 31. D Based on this, the detection unit 11 is initially in the cut-off region; as the voltage of G1 rises, the detection unit 11 will enter the saturation region from the cut-off region, and the second detection signal of the detection unit 11 will form an output signal curve that changes with the scanning voltage, such as Figure 4 As shown in FIG. 1 , D1 is the output signal curve of the first detection unit 11 in the first row. When the detection unit 11 enters the saturation region from the cut-off region, the detection unit 11 will switch from the cut-off state to the on state. Based on this, the detection circuit 30 can find out the second detection signal value when the detection unit 11 switches from the cut-off state to the on state from the output signal curve. At this time, the scanning signal voltage value corresponding to the second detection signal value is determined as the actual threshold voltage of the detection unit 11. The actual threshold voltage of the detection unit 11 is different from the standard threshold voltage V TH The difference is the threshold voltage change of the detection unit 11. According to the threshold voltage change of the detection unit 11, the pH value and / or ion concentration of the liquid at its site position can be calculated.

[0056] By analogy, the detection device can obtain the threshold voltage changes of multiple detection units 11 in batches, thereby realizing multi-site batch detection of the liquid to be tested.

[0057] refer to Figure 6 As shown, another detection signal schematic diagram provided by an embodiment of the present invention is shown in FIG. Figure 6 The scanning signal provided by the optional row scanning circuit to the scanning signal line is a sawtooth wave signal. Figure 7 FIG. 1 is a schematic diagram of another detection device provided by an embodiment of the present invention, Figure 7 The optional detection unit 11 shown includes a P-type field effect transistor, and the P-type field effect transistor has a standard threshold voltage V less than 0V. TH ; The sawtooth signal is from V GH Drop to V GL , where V GH Less than or equal to 0V and greater than V TH , V GL Less than V TH .

[0058] The first detection signal provided by the column detection circuit 30 to the first signal line 31 can be a constant DC signal V D The optional detection unit 11 includes a P-type field effect transistor, a constant DC signal V D Smaller than the standard threshold voltage V of a P-type field effect transistor TH .

[0059] Combination Figure 6 and Figure 7As shown, the scanning signal provided by the row scanning circuit 20 to the scanning signal line 21 is a sawtooth wave signal. The row scanning circuit 20 can optionally provide scanning signals to the m scanning signal lines 21 row by row. The scanning signals received by the m scanning signal lines 21 from top to bottom in sequence are marked as G1, G2, ..., Gm, all of which are sawtooth wave signals.

[0060] The detection unit 11 includes a P-type field effect transistor, and the P-type field effect transistor has a standard threshold voltage V less than 0V. TH The sawtooth wave signal is V GH When V GH Less than or equal to 0V and greater than V TH , so the P-type field effect transistor is in the cut-off region, and its state is cut-off state; the sawtooth wave signal is V GL When V GL Less than V TH , so the P-type field effect transistor is in the saturation region and its state is the on state. Based on this, it can be seen that when the sawtooth wave signal received by the control terminal c of the detection unit 11 changes from V GH Drop to V GL When , the P-type field effect transistor enters the saturation region from the cut-off region.

[0061] In this embodiment, when the detection device is immersed in the solution to be tested, the surface charge of the ion-sensitive layer of the detection unit 11 changes according to the different hydrogen ion (H+) concentrations of the solution at the site position, which will affect the threshold voltage of the detection unit 11. Then the critical point where the P-type field effect transistor in the detection unit 11 enters the saturation region from the cut-off region, that is, the threshold voltage, changes. It can be seen that the threshold voltage of the P-type field effect transistor may no longer be equal to the standard threshold voltage V TH .

[0062] Based on this, the actual threshold voltage of the detection unit 11 is calculated. It is known that the factor affecting the threshold voltage of the detection unit 11 is the hydrogen ion (H+) concentration of the solution at its site position. Therefore, the hydrogen ion (H+) concentration of the solution at its site position can be calculated based on the second detection signal output by the second signal line 32 regarding the threshold voltage change of the detection unit 11.

[0063] Combination Figure 6 and Figure 7 As shown, for example, in the detection phase of the first row detection unit 11, the row scanning circuit 20 provides a scanning signal G1 to the control terminal c of each detection unit 11 in the first row through the first scanning signal line 21, and G1 is a voltage from V GH Drop to V GL The column detection circuit 30 provides the first detection signal V to the n first signal lines 31. DBased on this, the detection unit 11 is initially in the cut-off region; as the voltage of G1 decreases, the detection unit 11 will enter the saturation region from the cut-off region, and the second detection signal of the detection unit 11 will form an output signal curve that changes with the scanning voltage, such as Figure 6 As shown in FIG. 1 , D1 is the output signal curve of the first detection unit 11 in the first row. When the detection unit 11 enters the saturation region from the cut-off region, the detection unit 11 switches from the cut-off state to the on state. Based on this, the detection circuit 30 can find out the second detection signal value when the detection unit 11 switches from the cut-off state to the on state from the output signal curve. At this time, the scanning signal voltage value corresponding to the second detection signal value is determined as the actual threshold voltage of the detection unit 11. The actual threshold voltage of the detection unit 11 is different from the standard threshold voltage V TH The difference is the threshold voltage change of the detection unit 11. According to the threshold voltage change of the detection unit 11, the pH value and / or ion concentration of the liquid at its site position can be calculated.

[0064] By analogy, the detection device can obtain the threshold voltage changes of multiple detection units 11 in batches, thereby realizing multi-site batch detection of the liquid to be tested.

[0065] refer to Figure 8 FIG. 1 is a schematic diagram of another detection device provided by an embodiment of the present invention, Figure 8 The optional detection device shown also includes: n electrical parameter acquisition units 33, the electrical parameter acquisition unit 33 is used to electrically connect to the column detection circuit 30 through a second signal line 32; the column detection circuit 30 is used to obtain a second detection signal corresponding to the second signal line 32 through the electrical parameter acquisition unit 33.

[0066] In this embodiment, the electrical parameter acquisition unit 33 is electrically connected to the column detection circuit 30 through a second signal line 32, so the column detection circuit 30 obtains the second detection signal corresponding to the second signal line 32 through the electrical parameter acquisition unit 33. The column detection circuit 30 determines the actual threshold voltage of the detection unit 11 according to the scanning signal, the second detection signal and the first detection signal of the detection unit 11, and then calculates the threshold voltage change. According to the threshold voltage change of the detection unit 11, the relevant parameters of the sample such as the liquid or droplet at the location of the detection unit 11 can be calculated. The relevant parameters can be pH value or ion concentration, and can also be other parameters of the sample such as the liquid.

[0067] refer to Fig. 9 FIG. 1 is a schematic diagram of another detection device provided by an embodiment of the present invention, Fig. 9 The optional electrical parameter acquisition unit 33 shown is a resistor, and the other end of the resistor is grounded. In other embodiments, the electrical parameter acquisition unit can also be a current sensor.

[0068] In this embodiment, the resistor 33 is electrically connected to the column detection circuit 30 through a second signal line 32, and the other end of the resistor 33 is grounded. As the scanning signal changes, when the detection unit 11 enters the saturation region from the cut-off region, the output current of the second end of the detection unit 11 changes, and the output current of the second end of the detection unit 11 flows through the resistor 33, which will cause the voltage division of the resistor 33 to change accordingly, so that the column detection circuit 30 can obtain a second detection signal through the second signal line 32, and the second detection signal is a voltage signal curve. In other words, if the resistor 33 is set in the circuit, the second detection signal obtained by the column detection circuit 30 is a voltage signal curve. It can be understood that the resistor 33 is only an example. In the actual circuit, when the second detection signal obtained by the column detection circuit 30 is a voltage signal curve, the circuit constructed includes but is not limited to the resistor 33, and also includes other auxiliary devices, which are not specifically exemplified here. The column detection circuit 30 determines the actual threshold voltage of the detection unit 11 based on the scanning signal, the second detection signal and the first detection signal of the detection unit 11, and then calculates the threshold voltage change. According to the threshold voltage change of the detection unit 11, the relevant parameters of the sample such as the liquid or droplet at the location of the detection unit 11 can be calculated. The relevant parameters can be pH value or ion concentration, or other parameters of the sample such as the liquid.

[0069] Based on the same inventive concept, an embodiment of the present invention further provides a detection method of a detection device, the detection device is the detection device described in any of the above embodiments, and the detection method of the detection device can achieve detection through the cooperation of a row scanning circuit and a column detection circuit. Fig.10 As shown in FIG. 1 , it is a schematic diagram of a detection method of a detection device provided by an embodiment of the present invention. Fig.10 As shown, the detection method of the detection device includes the following steps: S frame data acquisition page, S is greater than or equal to 1;

[0070] Step S10, in a frame of data acquisition page, the row scanning circuit provides scanning signals to m scanning signal lines row by row, and the column detection circuit provides first detection signals to n first signal lines; the column detection circuit simultaneously obtains second detection signals of a plurality of detection units in a row through a plurality of second signal lines;

[0071] Step S20, the detection phase includes at least one frame of data collection page. In the detection phase, the threshold drift of the detection unit is calculated according to the first detection signal, the second detection signal and the preset threshold voltage of the detection unit.

[0072] It can be understood that in a frame of data acquisition page, the row scanning circuit provides scanning signals to m scanning signal lines in time-sharing, the column detection circuit provides first detection signals to n first signal lines, and obtains the second detection signal of each detection unit through n second signal lines. The scanning mode of the row scanning circuit in a frame of data acquisition page can be row-by-row scanning, for example Figure 1 The progressive scanning from top to bottom shown can also be progressive scanning from bottom to top. In other embodiments, the scanning mode of the row scanning circuit in a frame of data acquisition page can also be interlaced scanning, for example, after the scanning of each odd row is completed, the scanning of each even row is performed, until the scanning of m scanning signal lines is completed. In other embodiments, the scanning mode of the row scanning circuit in a frame of data acquisition page can also be specified row scanning. It can be understood that the scanning mode of the row scanning circuit is not limited to progressive scanning and interlaced scanning, and relevant practitioners can also reasonably design the scanning mode of the row scanning circuit according to product requirements.

[0073] For any row in a frame of data acquisition page, the row scanning circuit provides a scanning signal to the scanning signal line, and the column detection circuit provides a first detection signal to multiple first signal lines among the n first signal lines, and the column detection circuit then obtains the second detection signal of multiple detection units in a row through multiple second signal lines. Batch data acquisition of multiple points in a row is realized, and individual data acquisition of any point in a row can also be realized.

[0074] After the acquisition is completed, the column detection circuit detects the acquired data. The detection phase includes at least one frame of data acquisition page. In the detection phase, the threshold drift of the detection unit is calculated according to the first detection signal, the second detection signal and the preset threshold voltage of the detection unit.

[0075] The optional scanning signal is a sawtooth wave signal, and the first detection signal is a constant DC signal. The detection method includes:

[0076] In a frame of data acquisition page, the row scanning circuit provides a sawtooth wave signal to a scanning signal line, and the column detection circuit provides a first detection signal to n first signal lines; then the column detection circuit simultaneously obtains the second detection signal of a row of multiple detection units through multiple second signal lines, wherein the second detection signal is an output signal curve that changes with the scanning signal. The column detection circuit determines the second detection signal value of the detection unit entering the saturation region from the cut-off region based on the output signal curve of the detection unit, and the scanning signal voltage corresponding to the second detection signal value is the actual threshold voltage of the detection unit; then the difference between the actual threshold voltage of the detection unit and the preset threshold voltage is the threshold drift amount of the detection unit.

[0077] According to the threshold drift of the detection unit, the pH value or ion concentration of the liquid at the location of the detection unit can be calculated.

[0078] The detection stage may include multiple frames of data acquisition pages. Then, for a detection unit, its threshold drift in each frame of data acquisition page can be obtained, and the average of the threshold drift can be taken. The liquid pH value or ion concentration at the location of the detection unit can be calculated based on the average of the threshold drift of the detection unit, which can improve the detection accuracy.

[0079] The detection method of the detection device provided by the embodiment of the present invention is that in a frame of data acquisition page, the row scanning circuit provides scanning signals to m scanning signal lines row by row, and the column detection circuit provides first detection signals to n first signal lines; the column detection circuit simultaneously obtains the second detection signals of multiple detection units in a row through multiple second signal lines; in the detection stage, the threshold drift amount of the detection unit is calculated according to the first detection signal, the second detection signal and the preset threshold voltage of the detection unit. Multi-site batch detection can be realized, and the detection efficiency and accuracy are improved.

[0080] refer to Fig.11 As shown in FIG. 1 , it is a schematic diagram of another detection method of a detection device provided by an embodiment of the present invention. Fig.11 The detection method shown optionally also includes: a self-calibration phase performed before the detection phase, S is greater than or equal to 2, and the self-calibration phase includes 1 frame of data acquisition page; step S01, in the self-calibration phase, calculating a preset threshold voltage of the detection unit.

[0081] During the working process of the detection device, the environmental parameters such as temperature and light may be different, which will make the standard threshold voltage of the detection unit in the detection device different. Based on this, a self-calibration phase is performed before the detection phase to obtain the preset threshold voltage of the detection unit in the current detection device, which can improve the detection accuracy.

[0082] It can be understood that at the same time, the environmental conditions of the detection units in the detection device are almost the same, so it can be considered that the preset threshold voltages are basically the same, so only one of the detection units needs to be self-calibrated to calculate the preset threshold voltage. After a period of time, the environmental conditions of the detection device may change, so the preset threshold voltage needs to be recalculated. Therefore, through the self-calibration stage, the accuracy of the preset threshold voltage of the detection unit can be improved, effectively avoiding the influence of characteristic differences and environmental factors between the detection units, and improving the detection accuracy.

[0083] After a certain time interval, the detection device performs a self-calibration phase before the detection phase to calculate the preset threshold voltage of the detection unit. In the detection phase, the threshold drift of the detection unit is calculated based on the first detection signal, the second detection signal and the preset threshold voltage of the detection unit, which can not only realize multi-site batch detection, but also improve the detection accuracy.

[0084] The operation of calculating the preset threshold voltage of the detection unit in the optional step S01 includes:

[0085] In the self-calibration stage, a first voltage curve of the detection unit is generated according to a second detection signal of the detection unit;

[0086] Calculate the first level value of the first voltage curve at the first preset ratio, and set the scanning signal voltage value corresponding to the first level value as the preset threshold voltage V of the detection unit. STH .

[0087] In this embodiment, combined with Figure 5 and Fig.11 , taking the first detection unit 11a in the first row as an example to describe the self-calibration stage, refer to Fig.12 Shown is a curve diagram of the detection unit during the self-calibration stage.

[0088] First, the row scanning circuit 20 provides a scanning signal G1 to the control terminal c of each detection unit 11 in the first row through the first scanning signal line 21. G1 is a voltage from V GL Rising to V GH Sawtooth wave signal.

[0089] Secondly, the column detection circuit 30 provides a first detection signal V to the first first signal line 31. D Then G1 is V GL When the detection unit 11a is initially in the cut-off region, the second detection signal is V SL As the voltage of G1 rises, the detection unit 11a will enter the saturation region from the cut-off region, and G1 is V GH When the second detection signal is V SH Therefore, the second detection signal of the detection unit 11a constitutes a first voltage curve D1 that changes with the scanning voltage, and the voltage value of D1 changes from V SL To V SH It should be noted that a first preset ratio is pre-designed in the column detection circuit 30, for example, the first preset ratio is at the 1 / 3 position of the first voltage curve D1, or the first preset ratio is the point where the slope of the first voltage curve D1 is the maximum, but it is not limited thereto, and relevant practitioners can reasonably design the first preset ratio.

[0090] Finally, the first level value of the first voltage curve D1 at the first preset ratio (1 / 3) is calculated, and the scanning signal voltage value corresponding to the first level value is set as the preset threshold voltage V of the detection unit 11a. STH It should be noted that the relevant practitioners design the first preset ratio by themselves, so the preset threshold voltage V of the detection unit in the detection device is STH It may not be equal to the threshold voltage value at which the detection unit enters the saturation region from the cut-off region.

[0091] The operation of calculating the threshold drift of the detection unit in the optional step S20 includes:

[0092] In the detection phase, a second voltage curve of the detection unit is generated according to a second detection signal of the detection unit;

[0093] Obtaining a second level value of the second voltage curve at a first preset ratio, and determining a scanning signal voltage value corresponding to the second level value of the detection unit as a first threshold voltage of the detection unit;

[0094] The difference between the first threshold voltage and the preset threshold voltage is determined as the threshold drift amount of the detection unit.

[0095] In this embodiment, combined with Figure 5 and Fig.11 , taking the first detection unit 11a in the first row as an example to describe the detection stage, refer to Fig.13 Shown is a curve schematic diagram of the detection unit during the detection stage.

[0096] First, the row scanning circuit 20 provides a scanning signal G1 to the control terminal c of each detection unit 11 in the first row through the first scanning signal line 21. G1 is a voltage from V GL Rising to V GH Sawtooth wave signal.

[0097] Secondly, the column detection circuit 30 provides a first detection signal V to the first first signal line 31. D Then G1 is V GL When the detection unit 11a is initially in the cut-off region, the second detection signal is V DL As the voltage of G1 rises, the detection unit 11a will enter the saturation region from the cut-off region, and G1 is V GH When the second detection signal is V DH Therefore, the second detection signal of the detection unit 11a constitutes a second voltage curve D2 that changes with the scanning voltage, and the voltage value of D2 changes from V DL To V DH .

[0098] It is known that the first preset ratio pre-designed in the column detection circuit 30 is at the 1 / 3 position of the first voltage curve D1. Then the second level value of the second voltage curve D2 at the first preset ratio (1 / 3) is calculated, and the scanning signal voltage value corresponding to the second level value is determined as the first threshold voltage V of the detection unit 11a. TH1 It should be noted that if the relevant practitioners design the first preset ratio by themselves, the first threshold voltage of the detection unit in the detection device may not be equal to the threshold voltage value of the detection unit entering the saturation region from the cut-off region.

[0099] Finally, the difference between the first threshold voltage and the preset threshold voltage is determined as the threshold drift amount of the detection unit.

[0100] By analogy, the detection device can obtain the threshold voltage drift of multiple detection units 11 in batches, thereby realizing multi-site batch detection of the liquid to be tested.

[0101] refer to Fig.14 As shown in FIG. 1 , it is a schematic diagram of a detection method of another detection device provided by an embodiment of the present invention. Fig.14 The detection method may further include: step S30, immersing the detection array in the liquid to be detected, and calculating the pH value or ion concentration of the liquid site where the detection unit is located according to the threshold drift of the detection unit, so as to realize batch detection of multiple sites of the sample.

[0102] In the embodiment of the present invention, a simple array-type detection unit structure is used to realize multi-site batch detection, thereby improving the sample detection efficiency. The detection method can be used for multi-site batch detection of a single sample, for example, the detection array of the detection device is immersed in the same liquid, and the threshold drift of multiple different detection units is calculated, thereby calculating the pH value or ion concentration of the same liquid at the liquid sites where the multiple detection units are located. The detection method can also be used for batch detection of multiple samples, for example, different liquid samples are dripped into different sites in the detection array of the detection device, and the threshold drift of multiple different detection units is calculated, thereby calculating the pH value or ion concentration of multiple samples.

[0103] Note that the above are only preferred embodiments of the present invention and the technical principles used. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments, combinations and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in more detail through the above embodiments, the present invention is not limited to the above embodiments, and may include more other equivalent embodiments without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A detection device, characterized in that: include: A detection array, comprising m*n detection units; A row scanning circuit and m scanning signal lines, wherein one scanning signal line is electrically connected to the control end of a row of detection units, and the row scanning circuit is used to provide scanning signals to the m scanning signal lines in a time-sharing manner; A column detection circuit, n first signal lines and n second signal lines, wherein one first signal line is electrically connected to a first end of a column of detection units, and one second signal line is electrically connected to a second end of a column of detection units, wherein the column detection circuit is used to provide a first detection signal to the n first signal lines, and simultaneously obtain second detection signals of a row of a plurality of detection units through a plurality of the second signal lines; During the detection process, the threshold voltage of the detection unit changes according to the concentration of hydrogen ions in the solution to be tested at the site, and the pH value of the solution at the site is determined according to the change in the threshold voltage; The scanning signal provided by the row scanning circuit to the scanning signal line is a sawtooth wave signal.

2. The detection device according to claim 1, characterized in that: The column detection circuit includes 1 first detection signal terminal and n second detection signal terminals; The first detection signal end is electrically connected to the n first signal lines, and is used to simultaneously provide the first detection signal to the n first signal lines; One of the second detection signal terminals is electrically connected to one of the second signal lines and is used to collect a second detection signal from the second signal line.

3. The detection device according to claim 1, characterized in that: The detection unit includes an N-type field effect transistor, and the N-type field effect transistor has a standard threshold voltage V greater than 0V. TH ; The sawtooth wave signal is from V GL Rising to V GH , wherein the V GL is less than or equal to 0V, and the V GH Greater than the V TH .

4. The detection device according to claim 1, characterized in that: The detection unit includes a P-type field effect transistor, and the P-type field effect transistor has a standard threshold voltage V less than 0V. TH ; The sawtooth wave signal is from V GH Drop to V GL , wherein the V GH is less than or equal to 0V and greater than the V TH , the V GL is less than the V TH .

5. The detection device according to claim 1, characterized in that: The detection unit includes a dual-gate ion-sensitive field effect transistor; The dual-gate ion-sensitive field effect transistor comprises a control end, an input end and an output end. The control end of the dual-gate ion-sensitive field effect transistor is electrically connected to the scanning signal line, the input end is electrically connected to the first signal line, and the output end is electrically connected to the second signal line.

6. The detection device according to claim 1, characterized in that: The first detection signal provided by the column detection circuit to the first signal line is a constant DC signal.

7. The detection device according to claim 6, characterized in that: The detection unit includes an N-type field effect transistor, and the constant DC signal is greater than a standard threshold voltage of the N-type field effect transistor; or, The detection unit includes a P-type field effect transistor, and the constant DC signal is smaller than a standard threshold voltage of the P-type field effect transistor.

8. The detection device according to claim 1, characterized in that: Also includes: n electrical parameter acquisition units, each of which is used to be electrically connected to the column detection circuit via one of the second signal lines; The column detection circuit is used to obtain a second detection signal corresponding to the second signal line through the electrical parameter acquisition unit.

9. The detection device according to claim 8, characterized in that: The electrical parameter acquisition unit is a resistor, and the other end of the resistor is grounded; or, The electrical parameter acquisition unit is a current sensor.

10. A detection method for a detection device, characterized in that: The detection method of the detection device according to any one of claims 1 to 9 comprises: S frame data acquisition pages, S is greater than or equal to 1; In one frame of the data acquisition page, the row scanning circuit provides scanning signals to the m scanning signal lines row by row, and the column detection circuit provides first detection signals to the n first signal lines; the column detection circuit simultaneously obtains second detection signals of a plurality of detection units in a row through a plurality of the second signal lines; The detection method also includes a detection phase, which includes at least one frame of the data acquisition page. In the detection phase, the threshold drift amount of the detection unit is calculated according to the first detection signal, the second detection signal and the preset threshold voltage of the detection unit.

11. The detection method according to claim 10, characterized in that: The scanning signal is a sawtooth wave signal, and the first detection signal is a constant direct current signal.

12. The detection method according to claim 10, characterized in that: Also includes: A self-calibration phase performed before the detection phase, S is greater than or equal to 2, and the self-calibration phase includes 1 frame of the data acquisition page; In the self-calibration stage, a preset threshold voltage of the detection unit is calculated.

13. The detection method according to claim 12, characterized in that: Calculating the preset threshold voltage of the detection unit includes: In the self-calibration stage, a first voltage curve of the detection unit is generated according to the second detection signal of the detection unit; Calculate the first level value of the first voltage curve at the first preset ratio, and set the scanning signal voltage value corresponding to the first level value as the preset threshold voltage V of the detection unit. STH .

14. The detection method according to claim 13, characterized in that: Calculating the threshold drift of the detection unit includes: In the detection phase, a second voltage curve of the detection unit is generated according to a second detection signal of the detection unit; obtaining a second level value of the second voltage curve at the first preset ratio, and determining a scanning signal voltage value corresponding to the second level value of the detection unit as a first threshold voltage of the detection unit; The difference between the first threshold voltage and the preset threshold voltage is determined as the threshold drift amount of the detection unit.

15. The detection method according to claim 10, characterized in that: Also includes: The detection array is immersed in the liquid to be detected, and the pH value or ion concentration of the liquid site where the detection unit is located is calculated according to the threshold drift of the detection unit.

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