A neurovascular signal detection device

Abstract

The present application discloses a neurovascular signal detection device, including a detection box, the inner wall of which is provided with a crawling surface for the activity of an experimental mouse, the crawling surface being hemispherical; a cover plate, which is detachably fixed at the top opening of the detection box; a mounting area is provided on the bottom surface of the cover plate at the center of the crawling surface; a control module is provided on the cover plate; a lightweight backpack for wearing on the body of the experimental mouse to be tested; a detection area is provided on the head of the experimental mouse; an electroencephalogram signal acquisition module is provided on the backpack for collecting electroencephalogram signals in the detection area; a tracking frame assembly, a laser emission module, an image acquisition module and a control module. The present application enables the detection data of the detection device to basically fit the normal activity state of the experimental mouse and be more accurate.

Description

Technical Field

[0001] The present disclosure generally relates to the field of experimental detection technology, and specifically to a neurovascular signal detection device. Background Art

[0002] Blood flow velocity is one of the most important functional metabolic parameters in the process of life activities. Dynamic monitoring of the spatiotemporal changes in blood flow velocity is of great significance for studying the physiological changes of biological tissues, functional activities and drug efficacy evaluation. Laser speckle blood flow imaging technology is a new rapid blood flow detection method that has emerged in recent years. It has the advantages of optical imaging without ionizing radiation and non-contact measurement. This technology uses the backward speckle of biological tissue to obtain relative blood flow velocity information. Through imaging, full-field two-dimensional high-resolution blood flow distribution imaging is obtained without the need for mechanical scanning; there is no need to inject exogenous substances such as contrast agents, and long-term continuous blood flow detection can be achieved.

[0003] When conducting the above experiment, the existing experimental detection device restricts the movement of the experimental mouse so that the detection device and the experimental mouse remain stationary. This method is not in a normal state for the experimental mouse, and the detection data is inaccurate. There are also methods of testing by having the rat carry a portable detection device on its back. Since the detection device has a certain weight, it will also affect the normal state of the experimental mouse and the accuracy of the detection data. Summary of the invention

[0004] In view of the above-mentioned defects or deficiencies in the prior art, it is desirable to provide a neurovascular signal detection device, comprising:

[0005] The detection box has an inner wall provided with a crawling surface for the experimental mice to move, and the crawling surface is hemispherical;

[0006] A cover plate is detachably fixed to the top opening of the detection box; a mounting area is provided on the bottom surface of the cover plate at the center of the crawling surface; a control module is provided on the cover plate;

[0007] A lightweight backpack is used to be worn on the body of the experimental mouse to be tested; the head of the experimental mouse is provided with a detection area; the backpack is provided with an EEG signal acquisition module, which is used to collect EEG signals in the detection area and send them to the control module;

[0008] Tracking frame assembly, including:

[0009] A universal ball, rotatably clamped in the installation area of ​​the cover plate;

[0010] A support rod having a first end connected to the universal ball and a second end opposite to the first end;

[0011] A connecting rod, two ends of which are respectively connected to the second end and an end of the edge of the back plate close to the detection area;

[0012] A detection rod is located above the connecting rod, one end of which is fixed to the outer wall of the supporting rod, and the other end is located above the detection area; a laser emission module and an image acquisition module are provided at one end of the detection rod away from the supporting rod;

[0013] The laser emitted by the laser emission module is irradiated obliquely onto the head of the experimental subject to form speckles thereon; the image acquisition module is used to acquire a cerebral cortical blood flow image corresponding to the speckle position and send it to the control module.

[0014] According to the technical solution provided in the embodiment of the present application, the universal ball has a first hollow cavity, and the support rod has a second through cavity connected to the first hollow cavity;

[0015] A self-winding measuring tape is installed in the first hollow cavity, and the end of the measuring tape extends from the first hollow cavity into the second through cavity and extends out from the second end, and is universally rotatably clamped with the top of the back plate.

[0016] According to the technical solution provided in the embodiment of the present application, the end of the detection rod away from the support rod is provided with:

[0017] A detection plate, fixedly connected to the end of the detection rod;

[0018] A micro motor is fixed on the bottom surface of the detection plate, and its output shaft is perpendicular to the detection plate and extends to a side away from the detection plate;

[0019] A rotating disk, the top center of which is vertically fixedly connected to the output shaft; the edge of the rotating disk close to the detection plate is rotatably clamped on the detection plate with the output shaft as the axis;

[0020] The laser emission module includes a first laser emission module and a second laser emission module, both of which are fixed on the side of the turntable away from the micro motor and are symmetrically arranged around the center of the turntable; the first laser emission module is used to emit a first laser signal; and the second laser emission module is used to emit a second laser signal.

[0021] According to the technical solution provided in the embodiment of the present application, the image acquisition module is installed on the detection rod, and the lens is arranged toward the detection area.

[0022] According to the technical solution provided in the embodiment of the present application, a control module is provided on the cover plate, and the control module is connected to the micro motor signal for controlling the forward and reverse rotation of the motor so that the first laser emission module and the second laser emission module are alternately aligned with the detection area.

[0023] According to the technical solution provided in the embodiment of the present application, the backpack plate is a plastic plate.

[0024] According to the technical solution provided in the embodiment of the present application, the wavelength of the first laser signal is 530 nm; the wavelength of the second laser signal is 605 nm.

[0025] In the technical solution of the present application, a hemispherical crawling surface is arranged in the detection box, and an installation area is provided on the cover plate at the center of the crawling surface. By installing a universal ball and supporting rods, connecting rods, detection rods and other components in the installation area, the laser emission module and image acquisition module used for collection can continuously track above the detection area and move with the movement of the experimental mouse without exerting pressure on the experimental mouse, so that the detection data of the detection device of the present application basically fits the normal activity state of the experimental mouse and is more accurate.

[0026] In certain embodiments of the present application, by setting a turntable and a micro-motor, the laser emission module includes a first laser emission module and a second laser emission module, the first laser emission module and the second laser emission module are both fixed on the side of the turntable away from the micro-motor and are symmetrically arranged around the center of the turntable; the first laser emission module is used to emit a first laser signal; the second laser emission module is used to emit a second laser signal. The detection of multiple EEG signals of the same experimental subject is achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Other features, objects and advantages of the present application will become more apparent by reading the detailed description of non-limiting embodiments made with reference to the following drawings:

[0028] Figure 1 It is a structural schematic diagram of this embodiment;

[0029] Figure 2 Schematic diagram of the installation structure of the laser emission module in this embodiment.

[0030] Numbers in the figure: 10 detection box; 20 cover plate; 40 lightweight backpack; 50 experimental mouse; 51 detection area; 61 universal ball; 61-2 measuring tape; 62 support rod; 63 connecting rod; 71 EEG signal acquisition module; 72 control module; 73 laser emission module; 74 image acquisition module; 73-1 first laser emission module; 73-2 second laser emission module; 61-1 first hollow cavity; 62-1 second through cavity; 66 micro motor; 65 detection plate; 67 turntable. DETAILED DESCRIPTION

[0031] The present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the relevant invention, rather than to limit the invention. It is also necessary to explain that, for ease of description, only the parts related to the invention are shown in the accompanying drawings.

[0032] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0033] Please refer to Figure 1 This embodiment provides a neurovascular signal detection device, including:

[0034] The inner wall of the detection box 10 is provided with a crawling surface 11 for the laboratory mouse to move, and the crawling surface 11 is hemispherical;

[0035] The cover plate 20 is detachably fixed to the top opening of the detection box 10; the bottom surface of the cover plate 20 is provided with a mounting area at the center of the crawling surface 11; the cover plate 20 is provided with a control module 72;

[0036] The light-weight backpack 40 is used to be worn on the body of the experimental mouse 50 to be tested; the head of the experimental mouse 50 is provided with a detection area 51; the backpack 40 is provided with an EEG signal acquisition module 71, which is used to collect EEG signals in the detection area and send them to the control module 72. The backpack is a plastic board or a foam board.

[0037] Tracking frame assembly, including:

[0038] A universal ball 61 rotatably clamped in the installation area of ​​the cover plate 20;

[0039] A support rod 62 having a first end connected to the universal ball 61 and a second end opposite to the first end;

[0040] A connecting rod 63, two ends of which are respectively connected to the second end and an end of the edge of the back plate 40 close to the detection area 51;

[0041] A detection rod is located above the connecting rod 63, one end of which is fixed to the outer wall of the support rod 62, and the other end is located above the detection area 51; a laser emission module 73 and an image acquisition module 74 are provided at one end of the detection rod away from the support rod 62;

[0042] The laser emitted by the laser emitting module 73 is irradiated obliquely onto the head of the experimental subject and forms speckles thereon; the image acquisition module 74 is used to acquire the cerebral cortex blood flow image corresponding to the speckle position.

[0043] The first set range on the head of the experimental object is set as the detection area, and the second set range in the detection area is set as the signal acquisition area. For example, a circular area with a diameter of 6 mm is selected as the detection area on the head of the experimental mouse, and the scalp and dura mater of the detection area are peeled off to expose the skull of the experimental area, and the glass cover is fixed against the skull surface of the experimental area. After the peeled area of ​​the experimental mouse recovers, a 3 mm circular area is selected in the experimental area as the signal acquisition area, and the skull of the signal acquisition area is peeled off to expose the cerebral cortex, i.e., brain tissue in the range. The laser emitted by the laser emission module 73 hits the cerebral cortex of the signal acquisition area and forms speckles thereon. The EEG signal acquisition module 71 is connected to the bottom of the glass cover through a probe, and the glass cover will press the acquisition line; when the speckle is formed, the image acquisition module 74 and the EEG signal acquisition module 71 synchronously collect data on the cerebral cortex of the signal acquisition area: the image acquisition module 74 obtains the cerebral cortex blood flow image on the cerebral cortex of the signal acquisition area; the laser signal passes through the glass cover to collect the image; the EEG signal acquisition module 71 is set on the cerebral cortex of the signal activity area to obtain the EEG signal of the area.

[0044] In this embodiment, the EEG signal acquisition module 71 uses a cerebus multi-channel neural signal recording system, which can record EEG signals in real time.

[0045] Preferably, the image acquisition module 74 uses a CMOS camera; the laser emission module 73 is configured as a laser diode, and the laser diode model is HL6322G; and the control module 72 uses an embedded ARM system.

[0046] In this embodiment, the laser emission module 73 includes a first laser emission module 73-1 and a second laser emission module 73-2, wherein the first laser emission module is used to emit a first laser signal; and the second laser emission module is used to emit a second laser signal. The wavelength of the first laser signal is 530nm; and the wavelength of the second laser signal is 605nm. The speckle image formed by the first laser signal irradiating the detection area can be used to extract the deoxyhemoglobin signal, and the speckle image formed by the second laser signal irradiating the detection area can be used to extract the blood flow velocity signal. Using MATLAB to extract the deoxyhemoglobin signal and the blood flow velocity signal from the speckle image is a prior art and will not be described in detail here.

[0047] In this embodiment, when the experimental mouse moves on the crawling surface, since the crawling surface is a hemispherical surface, the distance between the center of the back plate and the installation area remains unchanged, so the experimental mouse can walk freely in the detection box, and since the various modules used for detection are all installed on the detection rod connected to the support rod, the experimental mouse will not need to carry the weight of each module, and the detection data will be more accurate.

[0048] In some preferred embodiments of the present application, the universal ball 61 has a first hollow cavity 61 - 1 , and the support rod 62 has a second through cavity 62 - 1 communicating with the first hollow cavity 61 - 1 ;

[0049] A self-winding measuring tape 61 - 2 is installed in the first hollow cavity. The end of the measuring tape 61 - 2 extends from the first hollow cavity 61 - 1 into the second through cavity 62 - 1 and extends from the second end, and is universally rotatably clamped to the top of the back plate 40 .

[0050] In this embodiment, the connection stability between the tracking frame assembly and the experimental mouse is further enhanced by the self-winding measuring tape 61-2. Preferably, the measuring portion of the measuring tape is made of a flexible woven material.

[0051] In some preferred embodiments of the present application, the end of the detection rod away from the support rod 62 is provided with:

[0052] A detection plate 65, fixedly connected to the end of the detection rod;

[0053] A micro motor 66 is fixed on the bottom surface of the detection plate 65, and its output shaft is perpendicular to the detection plate 65 and extends to a side away from the detection plate 65;

[0054] The top center of the rotating disk 67 is vertically fixedly connected to the output shaft; the edge of the rotating disk 67 close to the detection plate 65 is rotatably clamped on the detection plate 65 with the output shaft as the axis;

[0055] The first laser emitting module 73-1 and the second laser emitting module 73-2 are both fixed on the side of the turntable away from the micro motor, and are symmetrically arranged around the center of the turntable;

[0056] In this embodiment, the first laser emitting module 73-1 and the second laser emitting module 73-2 are controlled to rotate the turntable so that the first laser emitting module 73-1 and the second laser emitting module 73-2 emit laser signals alternately corresponding to the detection area. When the image acquisition device respectively acquires the speckle image irradiated by the first laser signal and the speckle image irradiated by the second laser signal, the control module is connected to the micro-motor signal to control the forward and reverse rotation of the motor so that the first laser emitting module and the second laser emitting module are alternately aligned with the detection area; the control module 72 extracts the deoxyhemoglobin signal and the blood flow velocity signal from the two speckle images respectively.

[0057] In certain preferred embodiments of the present application, the image acquisition module 74 is installed on the detection rod, and the lens is arranged toward the detection area.

[0058] The above description is only a preferred embodiment of the present application and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the present application is not limited to the technical solution formed by a specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the inventive concept. For example, the above features are replaced with (but not limited to) technical features with similar functions disclosed in the present application.

Claims

1. A neurovascular signal detection device, characterized in that: include: The detection box has an inner wall provided with a crawling surface for the experimental mice to move, and the crawling surface is hemispherical; A cover plate is detachably fixed to the top opening of the detection box; a mounting area is provided on the bottom surface of the cover plate at the center of the crawling surface; a control module is provided on the cover plate; A lightweight backpack is used to be worn on the body of the experimental mouse to be tested; the head of the experimental mouse is provided with a detection area; the backpack is provided with an EEG signal acquisition module, which is used to collect EEG signals in the detection area and send them to the control module; Tracking frame assembly, including: A universal ball, rotatably clamped in the installation area of ​​the cover plate; A support rod having a first end connected to the universal ball and a second end opposite to the first end; A connecting rod, two ends of which are respectively connected to the second end and an end of the edge of the back plate close to the detection area; A detection rod is located above the connecting rod, one end of which is fixed to the outer wall of the supporting rod, and the other end is located above the detection area; a laser emission module and an image acquisition module are provided at one end of the detection rod away from the supporting rod; The laser emitted by the laser emission module is irradiated obliquely onto the head of the experimental subject to form speckles thereon; the image acquisition module is used to acquire a cerebral cortical blood flow image corresponding to the speckle position and send it to the control module; The universal ball has a first hollow cavity, and the support rod has a second through cavity connected to the first hollow cavity; A self-winding measuring tape is installed in the first hollow cavity, the end of the measuring tape extends from the first hollow cavity into the second through cavity, and extends from the second end, and is universally rotatably connected to the top of the backpack; the measuring tape is used to enhance the connection stability between the tracking frame assembly and the experimental mouse.

2. The neurovascular signal detection device according to claim 1, characterized in that: The end of the detection rod away from the support rod is provided with: A detection plate, fixedly connected to the end of the detection rod; A micro motor is fixed on the bottom surface of the detection plate, and its output shaft is perpendicular to the detection plate and extends to a side away from the detection plate; A rotating disk, the top center of which is vertically fixedly connected to the output shaft; an edge of the rotating disk close to one side of the detection plate is rotatably clamped on the detection plate with the output shaft as the axis; The laser emission module includes a first laser emission module and a second laser emission module, the first laser emission module and the second laser emission module are both fixed on the side of the turntable away from the micro motor and are symmetrically arranged around the center of the turntable; the first laser emission module is used to emit a first laser signal; the second laser emission module is used to emit a second laser signal.

3. The neurovascular signal detection device according to any one of claims 1 to 2, characterized in that: The image acquisition module is installed on the detection rod, and the lens is arranged toward the detection area.

4. The neurovascular signal detection device according to claim 2, characterized in that: A control module is disposed on the cover plate; the control module is connected to the micro motor signal and is used to control the motor to rotate forward and reverse so that the first laser emitting module and the second laser emitting module are alternately aligned with the detection area.

5. The neurovascular signal detection device according to any one of claims 1 to 2, characterized in that: The back plate is a plastic plate.

6. The neurovascular signal detection device according to claim 2, characterized in that: The wavelength of the first laser signal is 530 nm; the wavelength of the second laser signal is 605 nm.

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