Lysis assembly and tissue dissociation device

Abstract

The application discloses a kind of splitting assembly and tissue dissociation device, the splitting assembly includes dissociation cylinder and splitting knife, the dissociation cylinder has a dissociation cavity, the dissociation cavity is used to hold dissociation liquid with tissue, the bottom wall of the dissociation cavity is provided with a plurality of fixed blades, a plurality of the fixed blades are arranged along the circumference, the splitting knife is rotatably installed in the dissociation cavity along the axis extending upwards and downwards, the splitting knife includes at least two first blades spaced along the circumference and spirally arranged along the upwards and downwards, the splitting gap is formed between two the first blades and a plurality of the fixed blades, two the first blades and a plurality of the fixed blades cooperate to grind the tissue block in the splitting gap to obtain homogenate solution. By controlling the rotation speed and direction of the splitting knife, different effects can be achieved with a plurality of the fixed blades. When rotating forward, the tissue is broken, and when rotating backward, the tissue is incubated. The effect of tissue block splitting can be improved.

Description

Technical Field

[0001] This invention relates to the field of cell dissociation technology, and particularly to lysis components and tissue dissociation devices. Background Technology

[0002] Single-cell sequencing is a new technology that performs high-throughput sequencing analysis of the genome, transcriptome, and epigenome at the level of a single cell. It can reveal the gene structure and gene expression status of a single cell, reflecting the heterogeneity between cells, and plays an important role in fields such as oncology, developmental biology, microbiology, and neuroscience. Obtaining single-cell suspensions is a key focus of current technological research and improvement. Summary of the Invention

[0003] The main objective of this invention is to provide a pyrolysis component and a tissue dissociation device, which aims to pyrolyze tissue blocks in a dissociation solution to obtain a homogenized solution.

[0004] To achieve the above objectives, the present invention provides a pyrolysis assembly, comprising:

[0005] A dissociation cylinder having a dissociation chamber for holding a dissociation fluid containing tissue, the bottom wall of the dissociation chamber being provided with a plurality of fixed blades arranged circumferentially; and,

[0006] A lysis blade is rotatably mounted in the dissociation chamber along an axis extending vertically. The lysis blade includes at least two first blades spaced circumferentially and spirally arranged vertically. A lysis gap is formed between the two first blades and a plurality of fixed blades. The two first blades and the plurality of fixed blades cooperate to grind the tissue block in the lysis gap to obtain a homogenized solution.

[0007] Optionally, each of the fixed blades has a first grinding surface and a second grinding surface arranged at an angle. The first grinding surface cooperates with the two first blades to grind when the lysis blade rotates forward, and the second grinding surface cooperates with the two first blades to lift the tissue when the lysis blade rotates backward.

[0008] Optionally, the angle between the first grinding surface and the horizontal plane is a1, and the angle between the second grinding surface and the horizontal plane is a2, wherein a1 > 90° ≥ a2.

[0009] Optionally, the bottom wall of the dissociation cavity is recessed downwards at its center to form a groove, the inner sidewall of the groove is inclined downwards, and a plurality of the fixing blades are disposed on the inner sidewall of the groove; and / or,

[0010] The two adjacent fixed blades are set at an angle, forming an angle b, where 5°≤b≤30°.

[0011] Optionally, the plurality of fixed blades are arranged circumferentially and stacked sequentially in the vertical direction, and each of the first blades rotates along the vertically extending axis and can move vertically.

[0012] Optionally, the height of the rupture gap along the vertical direction is 0.05-5 mm; and / or,

[0013] The distance between each of the first blades and the inner wall of the dissociation chamber is 0.05-5 mm.

[0014] Optionally, the bottom of the dissociation cylinder is provided with a mounting hole communicating with the dissociation cavity;

[0015] The pyrolysis blade also includes a mounting post, the lower end of which is rotatably mounted into the mounting hole and sealed with the mounting hole;

[0016] Two of the first blades are located on the outer side of the mounting post.

[0017] Optionally, a material passage is formed in the middle of the upper end face of the mounting column, and a plurality of material passages communicating with the material passage are formed in the outer side of the lower end of the mounting column. The plurality of material passages are distributed circumferentially at intervals, and the interior of the material passage is used for a liquid extraction pipe to pass through so as to export the homogenized solution obtained by grinding from the plurality of material passages.

[0018] Optionally, an arc-shaped groove is formed on the lower end face of the first blade near the end of the mounting post, and the arc-shaped groove is provided through both ends in the circumferential direction;

[0019] The bottom wall of the dissociation chamber is provided with an arc-shaped guide plate, which slides along the arc-shaped groove when the pyrolysis blade rotates.

[0020] The present invention also proposes a tissue dissociation device, comprising:

[0021] Liquid container;

[0022] A pyrolysis assembly, wherein the dissociation cylinder and the liquid-holding cylinder are spaced apart; and...

[0023] A liquid extraction pipe, one end of which extends into the dissociation cylinder and the other end of which extends into the liquid holding cylinder, to introduce the homogenized solution between the inner wall of the dissociation chamber and the pyrolysis blade into the liquid holding cylinder.

[0024] In the technical solution of this invention, the dissociation solution containing tissue blocks is placed into the dissociation cylinder, driving the lysis blade to rotate. This causes the two first blades to cooperate with the inner wall of the dissociation cylinder and the multiple fixed blades to grind the tissue blocks together, crushing them. The dissociation solution dissolves the middle layer of the cell wall (pectin), separating the cells and facilitating subsequent steps. By controlling the rotation speed and direction of the lysis blade, different effects can be achieved with the multiple fixed blades. Rotating clockwise breaks up the tissue, while rotating counterclockwise incubates the tissue, thus turning the tissue blocks into a homogenized solution. This structure, through the cooperation of the rotating lysis blade and the fixed blades, can improve the lysis effect of the tissue blocks. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0026] Figure 1 A three-dimensional schematic diagram of an embodiment of the tissue dissociation device provided by the present invention;

[0027] Figure 2 for Figure 1 A schematic diagram of the cross-section of the tissue dissociation device along AA;

[0028] Figure 3 for Figure 1 A cross-sectional schematic diagram of the dissociation cylinder (at an angle);

[0029] Figure 4 for Figure 3 A magnified schematic diagram of part B in the middle;

[0030] Figure 5 for Figure 1 A cross-sectional view of the dissociation cylinder (from another angle);

[0031] Figure 6 for Figure 1 Top view of the dissociation cylinder;

[0032] Figure 7 for Figure 1 A three-dimensional schematic diagram of the pyrolysis blade (at an angle) in the dissociation cylinder;

[0033] Figure 8 for Figure 7 Top view of the splitting blade;

[0034] Figure 9 for Figure 1A three-dimensional schematic diagram of the pyrolysis blade (from another angle).

[0035] Explanation of icon numbers:

[0036]

[0037]

[0038] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0039] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0040] It should be noted that if the embodiments of the present invention involve directional indication, the directional indication is only used to explain the relative positional relationship and movement of the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0041] Furthermore, if the embodiments of this invention involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. If the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0042] Single-cell sequencing technology is a new technology that performs high-throughput sequencing analysis of the genome, transcriptome, and epigenome at the single-cell level. It can reveal the gene structure and gene expression status of a single cell, reflecting the heterogeneity between cells, and plays an important role in fields such as oncology, developmental biology, microbiology, and neuroscience. Obtaining single-cell suspensions is a key focus of current technological research and improvement.

[0043] In view of this, the present invention provides a pyrolysis component and a tissue dissociation device, which utilizes the cooperation of cutting tools to achieve tissue pyrolysis and preparation of a slurry solution. Figure 1 This is an embodiment of the tissue dissociation device provided by the present invention. Figures 2 to 9 An embodiment of the pyrolysis component provided by the present invention.

[0044] Please refer to Figures 1 to 2 The tissue dissociation device 1000 includes a lysis assembly 100, a liquid collection cylinder 200, and a liquid extraction pipe 300. The lysis assembly 100 is used to lyse the dissociation solution containing tissue blocks to obtain a homogenized solution. The dissociation cylinder 1 of the lysis assembly 100 is spaced apart from the liquid collection cylinder 200. One end of the liquid extraction pipe 300 extends into the dissociation cylinder 1, and the other end extends into the liquid collection cylinder 200 to introduce the homogenized solution between the inner wall of the dissociation chamber 11 and the lysis blade 2 into the liquid collection cylinder 200, thereby further processing the homogenized solution in the liquid collection cylinder 200.

[0045] Please refer to Figures 2 to 3 The lysis assembly 100 includes a dissociation cylinder 1 and a lysis blade 2. The dissociation cylinder 1 has a dissociation chamber 11, which is used to hold dissociation fluid containing tissue. The bottom wall of the dissociation chamber 11 is provided with a plurality of fixed blades 12, which are arranged circumferentially. The lysis blade 2 is rotatably mounted in the dissociation chamber 11 along an axis extending vertically. The lysis blade 2 includes at least two first blades 21 that are spaced circumferentially and spirally arranged vertically. A lysis gap is formed between the two first blades 21 and the plurality of fixed blades 12. The two first blades 21 and the plurality of fixed blades 12 cooperate to grind the tissue block in the lysis gap to obtain a homogenized solution.

[0046] In the technical solution of this invention, the dissociation solution containing tissue blocks is placed into the dissociation cylinder 1, driving the lysis blade 2 to rotate. This causes the two first blades 21 to cooperate with the inner wall surface of the dissociation cylinder 1 and the multiple fixed blades 12 to grind the tissue blocks together, so that the tissue blocks are crushed. The dissociation solution can dissolve the middle layer of the cell wall (pectin), thereby separating the cells and facilitating subsequent steps. By controlling the rotation speed and direction of the lysis blade 2, different effects can be achieved with the multiple fixed blades 21. Rotating clockwise breaks up the tissue, while rotating counterclockwise incubates the tissue, thereby turning the tissue blocks into a homogenized solution. This structure, through the cooperation of the rotating lysis blade 2 and the fixed blades 12, can improve the lysis effect of the tissue blocks.

[0047] Furthermore, each of the fixed blades 12 has a first grinding surface 121 and a second grinding surface 122 arranged at an angle. The first grinding surface 121 cooperates with the two first blades 21 to grind when the lysis blade 2 rotates clockwise, and the second grinding surface 122 cooperates with the two first blades 21 to lift the tissue upward when the lysis blade 2 rotates counterclockwise. This gives each fixed blade 12 two working surfaces. When the lysis blade 2 rotates clockwise, the first blades 21 press the tissue block downward into the lysis gap, so that the tissue block is simultaneously subjected to the squeezing action of the first grinding surface 121 and the lower end of the first blades 21, thereby achieving the effect of lysis and fragmentation. After maintaining clockwise rotation for a certain period, the lysis blade 2 is driven to rotate counterclockwise. At this time, the first blades 21 and the second grinding surface 122 cooperate to lift the fragmented tissue block from the lysis gap. Maintaining counterclockwise rotation for a certain period ensures that the fragmented tissue block can be fully mixed with the dissociation fluid. When rotating clockwise again, other unbroken tissue blocks can be pressed downward, achieving the final lysis effect.

[0048] To ensure the effective mating of the first grinding surface 121 and the second grinding surface 122, the configuration of the first grinding surface 121 and the second grinding surface 122 is limited. For details, please refer to... Figure 4 The angle between the first grinding surface 121 and the horizontal plane is a1, and the angle between the second grinding surface 122 and the horizontal plane is a2, wherein a1 > 90° ≥ a2. That is, the first grinding surface 121 has a smaller inclination, and the second grinding surface 122 has a larger inclination. In this embodiment, the angles a1 and a2 are defined as angles relative to the same side.

[0049] In one embodiment, the bottom wall of the dissociation chamber 11 is recessed downward to form a groove, and the inner sidewall of the groove is inclined downward. A plurality of fixed blades 12 are disposed on the inner sidewall of the groove. At this time, the plurality of fixed blades 12 are all inclined towards the bottom center of the dissociation cylinder 1, and correspondingly, the lower outer edge of each first blade 21 has an inclined end face, facilitating the collection and compression of tissue blocks.

[0050] Furthermore, the plurality of fixed blades 12 can be arranged parallel to each other in sequence. In one embodiment of the present invention, please refer to... Figure 6 The two adjacent fixed blades 12 are arranged at an angle, forming an angle of b, where 5°≤b≤30°. Multiple fixed blades 12 are designed to intersect in pairs, with an outwardly expanding shape. The grinding surface near the center has a smaller area, while the grinding surface near the outer edge has a larger area.

[0051] Furthermore, each of the fixed blades 12 is inclined in the horizontal plane, specifically at an angle to the straight line perpendicular to the rotation axis of the pyrolysis blade 2.

[0052] It should be noted that the two related features mentioned above—the inclined inner wall of the groove and the angle between the adjacent fixed blades 12—can be set simultaneously or one of them can be set selectively. In this embodiment, setting both simultaneously is more effective.

[0053] In other embodiments, multiple fixed blades 12 are arranged circumferentially and stacked sequentially in the vertical direction to form a structure similar to a spiral staircase. In this case, each first blade 21 is designed to rotate along the vertical axis and move vertically. That is, when rotating forward, the first blade 21 moves downward simultaneously, forming a lower step engagement; when rotating in reverse, the first blade 21 moves upward simultaneously, forming an upper step engagement. In this embodiment, the engagement between the pyrolysis blade 2 and the fixed blade 12 should be a continuous up-down reciprocating rotational motion.

[0054] To ensure the effective grinding of the tissue blocks, in one embodiment, the vertical height of the lysis gap is 0.05-5mm. Reasonably controlling the gap size can prevent incomplete grinding caused by an excessively large gap, thus ensuring the lysis effect. In this embodiment, the distance between the cutting edge of the fixed blade 12 and the bottom of each of the first blades 21 is set to 0.1mm.

[0055] During the lysis process, the tissue block is not only ground by the combined action of two cutting tools, but also by the tissue that has not flowed to the bottom of the dissociation chamber 1 when the lysis blade 2 rotates in the reverse direction and moves upward, or when rotating in the forward direction. During this rotation, the tissue is ground by the combined action of the inner wall of the dissociation chamber 1 and the outer side of the first blade 21. In one embodiment, the distance between each first blade 21 and the inner wall of the dissociation chamber 11 is 0.05-5 mm. Reasonably controlling the gap size can avoid incomplete grinding caused by an excessively large gap, ensuring the lysis effect. In this embodiment, the distance between each first blade 21 and the inner wall of the dissociation chamber 11 is set to 0.1 mm.

[0056] It should be noted that the two related technical features mentioned above can be set simultaneously or one of them can be set selectively. In this embodiment, the distance between the blade of the fixed blade 12 and each of the first blades 21 is set to 0.1mm, that is, the pyrolysis gap is 0.1mm, and the distance between each of the first blades 21 and the inner wall surface of the dissociation cavity 11 is 0.1mm.

[0057] Please refer to Figures 7 to 9To enable the rotatable installation of the pyrolysis blade 2, the bottom of the dissociation cylinder 1 is provided with a mounting hole 13 communicating with the dissociation chamber 11. The pyrolysis blade 2 also includes a mounting post 22, the lower end of which is rotatably installed into the mounting hole 13 and sealed with it. The first blade 21 is located on the outer surface of the mounting post 22. This arrangement provides greater stability, and the snap-fit ​​fit restricts the relative position of the mounting post 22 and the dissociation cylinder 1. Simultaneously, the movable seal prevents leakage of the dissociation fluid.

[0058] After pyrolysis, the homogenized solution needs to be exported for further processing in the liquid container 200. At this time, if the dissociation container 1 is directly poured out, the homogenized solution may be stuck on the first blade 21, resulting in a large amount of residue. Therefore, in one embodiment of the present invention, a material passage 221 is formed on the upper end face of the mounting column 22, and a plurality of material ports 222 communicating with the material passage 221 are provided on the lower outer side of the mounting column 22. The plurality of material ports 222 are distributed circumferentially. The liquid extraction pipe 300 passes through the inside of the material passage 221 to export the homogenized solution obtained by grinding from the plurality of material ports 222. After pyrolysis, the homogenized solution is located between the inner wall of the mounting column 22 and the dissociation cylinder 1. At this time, the extraction pipe 300 is inserted into the material passage 221. During extraction, the homogenized solution first enters the material passage 221 through multiple material ports 222, then is sucked into the extraction pipe 300, and finally introduced into the liquid container 200. This arrangement can keep the dissociation cylinder 1 fixed at all times, and allows for quick and convenient extraction of the homogenized solution. It should be noted that by reasonably setting the gap between the extraction pipe 300 and the inner wall of the material passage 221, the extraction pipe 300 can also be inserted when the pyrolysis blade 2 rotates, saving operation steps.

[0059] It should be noted that each of the first blades 21 has a certain thickness along the radial direction of the mounting post 22. In one embodiment, the first blade 21 extends in a twisted manner along the same helical direction. In another embodiment, along the radial direction of the mounting post 22, each of the first blades 21 has a first side portion close to the mounting post 22 and a second side portion away from the mounting post 22, and the extension curves of the first side portion and the extension curves of the second side portion intersect. In this case, the first side portion and the second side portion extend in two helical directions respectively, so that the first blade 21 is twisted as a whole. Under this structure, the first blade 21 can increase the crushing degree of the tissue block during the rotation and lysis process, thereby obtaining a better lysis effect.

[0060] In one embodiment, the first blade 21 is configured such that its width gradually increases from top to bottom, thereby making the first blade 21 narrower at the top and wider at the bottom. This can prevent tissue blocks from being lifted up and accumulating in the upper part of the dissociation cylinder 1 when the mounting post 22 is reversed, while increasing the crushing degree below.

[0061] In another embodiment, the helix angle of the first blade 21 gradually increases from top to bottom. This makes the first blade 21 generally slope downwards, which can prevent tissue blocks from being lifted and accumulating in the upper part of the dissociation cylinder 1 when the mounting post 22 reverses, while increasing the crushing degree below.

[0062] It should be noted that the present invention does not limit the number of the first blades 21. In one embodiment, two first blades 21 are arranged symmetrically at the center. In another embodiment, multiple first blades 21 are provided and are evenly arranged circumferentially.

[0063] To facilitate the rotational guidance of the pyrolysis blade 2 within the dissociation cylinder 1, in this embodiment, an arc-shaped groove 211 is formed on the lower end face of the first blade 21 near the end of the mounting post 22. The arc-shaped groove 211 extends through both ends in the circumferential direction. An arc-shaped guide plate 14 is provided on the bottom wall of the dissociation cavity 11. The guide plate 14 slides along the arc-shaped groove 211 when the pyrolysis blade 2 rotates. The guide plate 14 remains fixed, while the arc-shaped groove 211 rotates with the mounting post 22, thereby achieving relative rotational engagement between the two and preventing the mounting post 22 from deflecting due to the presence of a gap during high-level rotation.

[0064] Furthermore, to improve the effectiveness of guidance coordination, please refer to... Figures 7 to 8 The first blade 21 has a rib 23 on one side along its circumferential direction. The rib 23 and the mounting post 22 together form a guide channel. The guide channel communicates with the arc-shaped groove 211, and both the side end and the bottom end of the guide channel are through-connected. The rib 23 is generally L-shaped. After the guide channel communicates with the arc-shaped groove 211, it extends the overall channel length that mates with the guide plate 14, thereby improving the stability of the mating.

[0065] Furthermore, the lower end of the mounting column 22 is tapered downwards to form an inclined surface, and multiple feed ports 222 are located on the inclined surface. This arrangement ensures that the extension direction of the feed ports 222 is inclined relative to the horizontal plane, which can prevent dissociation fluid and tissue blocks from entering the feed channel 221 through the feed ports 222 during the pyrolysis process, thus avoiding incomplete dissociation.

[0066] Based on the above structure, the specific steps for preparing a single-cell suspension are as follows:

[0067] Tissue preparation: Add 1×PBS solution to a six-well plate to wash the sample stored in the tissue preservation solution. Take an appropriate amount of the washed sample and add it to the dissociation solution. Use surgical scissors to cut the tissue into tissue blocks of appropriate size.

[0068] Tissue lysis: The dissociation solution containing the tissue is placed in the dissociation chamber 11 and treated at 37°C for 15-40 minutes. During this process, the lysis blade 2 needs to be rotated in the forward direction to break up the tissue every 5 minutes in the dissociation chamber 11. Then, the lysis blade 2 rotates in the reverse direction for the same amount of time to incubate the tissue. The optimal method is to completely digest the tissue block and form a homogenous solution.

[0069] Transfer of homogenate solution: The homogenate solution is subjected to negative pressure in the pumping pipe 300 and enters the liquid holding cylinder 200 after being filtered through a 40-70μm sterile cell sieve.

[0070] An equal volume of termination solution is added to the filtrate in the liquid container 200 to complete the dissociation termination and obtain a cell suspension.

[0071] Add an equal volume of erythrocyte lysis buffer to the cell suspension, invert to mix, and incubate at room temperature for 2-7 minutes.

[0072] Add an equal volume of 1640 solution to stop erythrocyte lysis, invert and mix well, then incubate at 300g for 5 minutes at 4°C. Remove the supernatant to obtain a single-cell precipitate, and resuspend to obtain a single-cell suspension.

[0073] The above description is merely a preferred embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the contents of the present invention's specification and drawings under the inventive concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A pyrolysis assembly, characterized in that, include: A dissociation cylinder having a dissociation chamber for holding a dissociation fluid containing tissue, the bottom wall of the dissociation chamber being provided with a plurality of fixed blades arranged circumferentially; and, A lysis blade is rotatably mounted in the dissociation chamber along an axis extending vertically. The lysis blade includes at least two first blades spaced circumferentially and spirally arranged vertically. A lysis gap is formed between the two first blades and a plurality of fixed blades. The two first blades and the plurality of fixed blades cooperate to grind the tissue block in the lysis gap to obtain a homogenized solution. The bottom of the dissociation cylinder is provided with a mounting hole that communicates with the dissociation cavity; The pyrolysis blade also includes a mounting post, the lower end of which is rotatably mounted into the mounting hole and sealed with the mounting hole; Two of the first blades are disposed on the outer side of the mounting post; An arc-shaped groove is formed on the lower end face of the first blade near the end of the mounting post, and the arc-shaped groove is provided through both ends in the circumferential direction; The bottom wall of the dissociation chamber is provided with an arc-shaped guide plate, which slides along the arc-shaped groove when the pyrolysis blade rotates.

2. The pyrolysis assembly as described in claim 1, characterized in that, Each of the fixed blades has a first grinding surface and a second grinding surface arranged at an angle. The first grinding surface cooperates with the two first blades to grind when the lysis blade rotates forward, and the second grinding surface cooperates with the two first blades to lift the tissue when the lysis blade rotates backward.

3. The pyrolysis assembly as described in claim 2, characterized in that, The angle between the first grinding surface and the horizontal plane is a1, and the angle between the second grinding surface and the horizontal plane is a2, wherein a1 > 90° ≥ a2.

4. The pyrolysis assembly as described in claim 1, characterized in that, The bottom wall of the dissociation chamber is recessed downwards in the middle to form a groove, the inner sidewall of the groove is inclined downwards, and a plurality of the fixed blades are disposed on the inner sidewall of the groove; and / or, The two adjacent fixed blades are set at an angle, forming an angle b, where 5°≤b≤30°.

5. The pyrolysis assembly as described in claim 1, characterized in that, The plurality of fixed blades are arranged circumferentially and stacked sequentially in the vertical direction. Each first blade rotates along an axis extending vertically and can move vertically.

6. The pyrolysis assembly as described in claim 1, characterized in that, The vertical height of the rupture gap is 0.05-5 mm; and / or, The distance between each of the first blades and the inner wall of the dissociation chamber is 0.05-5 mm.

7. The pyrolysis assembly as described in claim 1, characterized in that, A material passage is formed in the middle of the upper end face of the mounting column, and multiple material passages communicating with the material passage are provided on the lower outer side of the mounting column. The multiple material passages are distributed circumferentially. The interior of the material passage is used for a liquid extraction pipe to pass through so as to export the homogenized solution obtained by grinding from the multiple material passages.

8. A tissue dissociation device, characterized in that, include: Liquid container; The pyrolysis assembly as described in any one of claims 1 to 7, wherein the dissociation cylinder and the liquid holding cylinder are spaced apart in the pyrolysis assembly; as well as, A liquid extraction pipe, one end of which extends into the dissociation cylinder and the other end of which extends into the liquid holding cylinder, to introduce the homogenized solution between the inner wall of the dissociation chamber and the pyrolysis blade into the liquid holding cylinder.

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