Sample mixing simulation test method and device, sample mixing equipment and storage medium

Abstract

The present application provides a sample mixing simulation test method and apparatus, a sample mixing device and a storage medium, and relates to the field of biological sample detection technology. The present application obtains the first absorbance of a simulated sample that matches the viscosity of the sample to be tested at the maximum absorption wavelength of its own fluorescent material, the second absorbance of a simulated reagent that matches the viscosity of a preset reagent at the maximum absorption wavelength of its own fluorescent material, the third and fourth absorbances of a target viscosity solvent shared by the simulated reagent and the simulated sample at the two maximum absorption wavelengths, and the fifth and sixth absorbances of a mixed reaction liquid after the simulated sample and the simulated reagent are mixed by the sample reagent, respectively, at the two maximum absorption wavelengths, and then performs data analysis on the first, second, third, fourth, fifth and sixth absorbances to obtain corresponding mixing effect evaluation parameters, thereby avoiding meaningless loss of the sample to be tested and the reagent, and effectively and quantitatively evaluating the mixing effect of the specific sample reagent.

Description

Technical Field

[0001] The present application relates to the technical field of biological sample detection, and in particular to a sample mixing simulation test method and apparatus, a sample mixing device and a storage medium. Background Art

[0002] In vitro diagnostics (IVDs) are a key branch of modern medical technology. They enable the testing of human samples (e.g., blood, body fluids, tissues, etc.) outside the human body to obtain clinical diagnostic information and diagnose diseases or body functions. During the testing process, it is often necessary to thoroughly mix the sample with in vitro test reagents and, after applying certain reaction conditions, detect the photoelectric signal of the mixed liquid to obtain specific information about the analyte in the sample. However, poor mixing of the reagents and sample can affect the reaction process between the sample and reagents, leading to abnormal test results.

[0003] With the rapid development of automation technology, the rapid batch mixing of samples and reagents through automated testing equipment can effectively improve sample testing efficiency and has become one of the important development directions of in vitro diagnostic technology. Therefore, how to accurately understand the mixing effect of sample reagents and then verify and optimize the sample mixing performance of automated testing equipment is of great significance to ensure accurate and reliable sample test results. Summary of the Invention

[0004] In view of this, the purpose of this application is to provide a sample mixing simulation test method and device, sample mixing equipment and storage medium, which can effectively quantitatively evaluate the specific mixing effect of the mixing operation of the sample to be tested and the corresponding reagent while avoiding meaningless loss of the sample to be tested and the corresponding reagent, so as to facilitate the testing personnel to adjust the mixing operation details for the sample to be tested and the corresponding reagent, so that the final test results are accurate and reliable.

[0005] In order to achieve the above objectives, the technical solutions adopted in the embodiments of the present application are as follows:

[0006] In a first aspect, the present application provides a sample mixing simulation test method, the method comprising:

[0007] Obtaining a first absorbance of a simulated sample having a viscosity matching that of the sample to be tested at a first maximum absorption wavelength corresponding to the first fluorescent dye, wherein the simulated sample is obtained by dissolving the first fluorescent dye in a target viscosity solvent;

[0008] Obtaining a second absorbance of a simulation reagent matching a preset reagent viscosity at a second maximum absorption wavelength corresponding to a second fluorescent dye, wherein the simulation reagent is obtained by dissolving the second fluorescent dye in the target viscosity solvent;

[0009] Obtaining a third absorbance and a fourth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of the target viscous solvent, respectively;

[0010] Obtaining a fifth absorbance and a sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, of a mixed reaction solution obtained by performing a sample-reagent mixing operation on the simulated sample and the simulated reagent;

[0011] Data analysis is performed on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect evaluation parameter that matches the sample reagent mixing operation.

[0012] In an optional embodiment, the step of performing data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect evaluation parameter that matches the sample-reagent mixing operation includes:

[0013] calculating a first net absorption coefficient of the mixed reaction solution relative to the simulated sample during the sample-reagent mixing operation based on the first absorbance, the fifth absorbance, and the third absorbance;

[0014] calculating a second net absorption coefficient of the mixed reaction solution relative to the simulation reagent during the sample reagent mixing operation based on the second absorbance, the sixth absorbance, and the fourth absorbance;

[0015] A ratio operation is performed on the first net absorption coefficient and the second net absorption coefficient to obtain a mixing effect evaluation parameter that matches the sample reagent mixing operation.

[0016] In an optional embodiment, the step of calculating a first net absorption coefficient of the mixed reaction solution relative to the simulated sample under the sample reagent mixing operation based on the first absorbance, the fifth absorbance, and the third absorbance includes:

[0017] performing a subtraction operation on the fifth absorbance and the third absorbance to obtain a net absorbance of the mixed reaction solution at a first maximum absorption wavelength;

[0018] performing a subtraction operation on the first absorbance and the third absorbance to obtain a net absorbance of the simulated sample at a first maximum absorption wavelength;

[0019] A ratio operation is performed on the net absorbances of the mixed reaction solution and the simulated sample at the first maximum absorption wavelength to obtain the first net absorption coefficient.

[0020] In an optional embodiment, the step of calculating a second net absorption coefficient of the mixed reaction solution relative to the simulation reagent under the sample reagent mixing operation based on the second absorbance, the sixth absorbance, and the fourth absorbance includes:

[0021] performing a subtraction operation on the sixth absorbance from the fourth absorbance to obtain a net absorbance of the mixed reaction solution at a second maximum absorption wavelength;

[0022] performing a subtraction operation on the second absorbance and the fourth absorbance to obtain a net absorbance of the simulation reagent at a second maximum absorption wavelength;

[0023] A ratio operation is performed on the net absorbances of the mixed reaction solution and the simulation reagent at the second maximum absorption wavelength to obtain the second net absorption coefficient.

[0024] In an optional embodiment, the closer the mixing effect evaluation parameter is to the volume ratio between the simulated sample and the simulated reagent corresponding to the mixed reaction liquid, the better the mixing effect of the sample-reagent mixing operation.

[0025] In a second aspect, the present application provides a sample mixing simulation test device, the device comprising:

[0026] a simulated sample absorbance acquisition module, configured to acquire a first absorbance of a simulated sample having a viscosity matching that of the sample to be tested at a first maximum absorption wavelength corresponding to a first fluorescent dye, wherein the simulated sample is obtained by dissolving the first fluorescent dye in a target viscosity solvent;

[0027] a simulation reagent absorbance acquisition module, configured to acquire a second absorbance of a simulation reagent matching a preset reagent viscosity at a second maximum absorption wavelength corresponding to a second fluorescent dye, wherein the simulation reagent is obtained by dissolving the second fluorescent dye in the target viscosity solvent;

[0028] a viscous solvent absorption acquisition module, configured to acquire a third absorbance and a fourth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of the target viscous solvent, respectively;

[0029] a mixing operation absorbance acquisition module, configured to acquire a fifth absorbance and a sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, of a mixed reaction solution obtained by performing a sample-reagent mixing operation on the simulated sample and the simulated reagent;

[0030] The mixing effect evaluation and analysis module is used to perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance to obtain a mixing effect evaluation parameter that matches the sample reagent mixing operation.

[0031] In an optional embodiment, the mixing effect evaluation and analysis module includes:

[0032] a first net absorbance calculation submodule, configured to calculate a first net absorbance coefficient of the mixed reaction solution relative to the simulated sample under the sample reagent mixing operation based on the first absorbance, the fifth absorbance, and the third absorbance;

[0033] a second net absorbance calculation submodule, configured to calculate a second net absorbance coefficient of the mixed reaction solution relative to the simulation reagent under the sample reagent mixing operation based on the second absorbance, the sixth absorbance, and the fourth absorbance;

[0034] The mixing effect evaluation submodule is configured to perform a ratio operation on the first net light absorption coefficient and the second net light absorption coefficient to obtain a mixing effect evaluation parameter that matches the sample reagent mixing operation.

[0035] In an optional embodiment, the closer the mixing effect evaluation parameter is to the volume ratio between the simulated sample and the simulated reagent corresponding to the mixed reaction liquid, the better the mixing effect of the sample-reagent mixing operation.

[0036] In a third aspect, the present application provides a sample mixing device, comprising a processor and a memory, wherein the memory stores a computer program that can be executed by the processor, and the processor can execute the computer program to implement the sample mixing simulation test method described in any one of the aforementioned embodiments.

[0037] In a fourth aspect, the present application provides a storage medium having a computer program stored thereon. When the computer program is executed by a processor, the sample mixing simulation test method described in any one of the aforementioned embodiments is implemented.

[0038] In this case, the beneficial effects of the embodiments of the present application include the following:

[0039] The present application obtains a first absorbance of a simulated sample matching the viscosity of the sample to be tested at a first maximum absorption wavelength of the autofluorescent material, a second absorbance of a simulated reagent matching the viscosity of the preset reagent at a second maximum absorption wavelength of the autofluorescent material, a third absorbance and a fourth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of a target viscosity solvent shared by the simulated reagent and the simulated sample, and a fifth absorbance and a sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of a mixed reaction liquid after the simulated sample and the simulated reagent are mixed by the sample reagent, and then the first absorbance and the second absorbance are calculated. The data of the first absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance are analyzed to obtain mixing effect evaluation parameters that match the sample and reagent mixing operation, so as to use the simulated sample and the simulated reagent that actually participate in the mixing to replace the sample to be tested and the corresponding reagent respectively, thereby avoiding meaningless loss of the sample to be tested and the corresponding reagent. The specific mixing effect of the mixing operation of the sample to be tested and the preset reagent is effectively and quantitatively evaluated through the mixing effect evaluation parameters, which is convenient for testing the sample and reagent mixing performance of the automated sample testing equipment, and also helps the testing personnel to adjust and optimize the operation details when manually performing the sample and reagent mixing operation, so that the final test results are accurate and reliable.

[0040] In order to make the above-mentioned objects, features and advantages of the present application more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without creative work.

[0042] Figure 1 A schematic diagram of the composition of a sample mixing device provided in an embodiment of the present application;

[0043] Figure 2 A schematic diagram of a flow chart of a sample mixing simulation test method provided in an embodiment of the present application;

[0044] Figure 3 A schematic diagram of the absorbance distribution of the fifth and sixth absorbances under the sample reagent mixing operation with different mixing times provided in the embodiment of the present application;

[0045] Figure 4 for Figure 2 The flowchart of the sub-steps included in step S250 is shown;

[0046] Figure 5A schematic diagram of parameter distribution of mixing effect evaluation parameters under sample and reagent mixing operations with different mixing times provided in an embodiment of the present application;

[0047] Figure 6 A schematic diagram of the composition of a sample mixing simulation test device provided in an embodiment of the present application;

[0048] Figure 7 for Figure 6 Schematic diagram of the composition of the mixing effect evaluation and analysis module.

[0049] Icons: 10-sample mixing device; 11-memory; 12-processor; 13-communication unit; 14-mixing component; 100-sample mixing simulation test device; 110-simulated sample absorbance acquisition module; 120-simulated reagent absorbance acquisition module; 130-viscous solvent absorbance acquisition module; 140-mixing operation absorbance acquisition module; 150-mixing effect evaluation and analysis module; 151-first net absorbance calculation submodule; 152-second net absorbance calculation submodule; 153-mixing effect evaluation submodule. DETAILED DESCRIPTION

[0050] To make the objectives, technical solutions, and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Generally, the components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0051] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments in the present application without creative work are within the scope of protection of the present application.

[0052] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0053] In the description of the present application, it should be understood that relational terms such as the terms "first" and "second" are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprise", "include" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or also include elements inherent to such process, method, article or equipment. In the absence of further restrictions, the elements defined by the statement "comprise a ..." do not exclude the presence of other identical elements in the process, method, article or equipment comprising the elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0054] The applicant has found through painstaking research that the existing technical solutions for evaluating the mixing effect of sample reagents generally include: (1) subjective qualitative evaluation of the mixing effect by visual means, which is essentially unable to quantitatively evaluate the quality of the specific mixing effect and usually cannot guarantee the accuracy and reliability of the final test results; (2) using the whole machine to conduct reverse verification tests on the sample test results under different degrees of mixing operations from the perspective of clinical testing, thereby achieving an indirect evaluation of the specific mixing effect. The entire process requires the loss of a large amount of human samples and in vitro test reagents.

[0055] To this end, the applicant has developed a sample mixing simulation test method and device, a sample mixing device and a storage medium, which can effectively quantitatively evaluate the specific mixing effect of the mixing operation of the sample to be tested and the corresponding reagent while avoiding meaningless loss of the sample to be tested and the corresponding reagent. This is convenient for testing the sample reagent mixing performance of the automated sample testing equipment, and also helps the testing personnel to adjust and optimize the operation details when manually performing the sample reagent mixing operation, so that the final test results are accurate and reliable.

[0056] The following describes some embodiments of the present application in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments may be combined with each other.

[0057] Please refer to Figure 1 , Figure 1 Schematic diagram of the composition of the sample mixing device 10 provided in an embodiment of the present application. In this embodiment of the present application, the sample mixing device 10 is capable of performing a mixing operation on a sample to be processed and a reagent to be processed, and quantitatively evaluating the specific mixing effect of the mixing operation on the sample to be processed and the reagent to be processed. This facilitates the tester to adjust the mixing operation details for the sample to be processed and the reagent to be processed, ensuring that the final test results corresponding to the sample to be processed and the reagent to be processed are accurate and reliable.

[0058] In this embodiment, the sample mixing device 10 may include a memory 11, a processor 12, a communication unit 13, a mixing component 14, and a sample mixing simulation test device 100. The memory 11, the processor 12, the communication unit 13, and the mixing component 14 are electrically connected to each other, directly or indirectly, to enable data transmission or interaction. For example, the memory 11, the processor 12, the communication unit 13, and the mixing component 14 may be electrically connected to each other via one or more communication buses or signal lines.

[0059] In this embodiment, the memory 11 can be, but is not limited to, a random access memory (RAM), a read-only memory (ROM), a programmable read-only memory (PROM), an erasable programmable read-only memory (EPROM), an electrically erasable programmable read-only memory (EEPROM), etc. The memory 11 is used to store a computer program, and the processor 12 can execute the computer program accordingly after receiving an execution instruction. In addition, the memory 11 is also used to store a specific calculation formula for a mixing effect evaluation parameter, wherein the mixing effect evaluation parameter is used to represent the quality of the specific mixing effect of the corresponding sample and the corresponding reagent under the current mixing operation, wherein the closer the mixing effect evaluation parameter is to the volume ratio between the sample and the reagent participating in the mixing operation, the better the mixing effect of the mixing operation.

[0060] In this embodiment, the processor 12 can be an integrated circuit chip with signal processing capabilities. The processor 12 can be a general-purpose processor, including at least one of a central processing unit (CPU), a graphics processing unit (GPU), a network processor (NP), a digital signal processor (DSP), an application-specific integrated circuit (ASIC), a field-programmable gate array (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, and discrete hardware components. The general-purpose processor can be a microprocessor or any conventional processor, etc., which can implement or execute the various methods, steps, and logic block diagrams disclosed in the embodiments of the present application.

[0061] In this embodiment, the communication unit 13 is used to establish a communication connection between the sample mixing device 10 and other electronic devices via a network, and to send and receive data via the network, where the network includes both a wired communication network and a wireless communication network. For example, the sample mixing device 10 can receive a mixing control instruction from a control device via the communication unit 13 and perform a corresponding mixing operation according to the mixing duration specified in the mixing control instruction.

[0062] In this embodiment, the mixing component 14 is used for the mixing function of the sample mixing device 10 , wherein the mixing component 14 may include one or more combinations of a stirring component, an oscillating component, and an ultrasonic component.

[0063] In this embodiment, the sample mixing simulation test device 100 includes at least one software function module that can be stored in the memory 11 in the form of software or firmware or solidified in the operating system of the sample mixing device 10. The processor 12 can be used to execute the executable modules stored in the memory 11, such as the software function modules and computer programs included in the sample mixing simulation test device 100. The sample mixing device 10 can effectively perform quantitative evaluation on the specific mixing effect of the mixing operation of the sample to be tested and the corresponding reagent while avoiding the meaningless loss of the sample to be tested and the corresponding reagent through the sample mixing simulation test device 100, which is convenient for testing the sample reagent mixing performance of the automated sample detection equipment, and also helps the detection personnel to adjust and optimize the operation details when manually performing the sample reagent mixing operation, so that the final detection result is accurate and reliable.

[0064] It is understandable that Figure 1 The block diagram shown is only a schematic diagram of the composition of the sample mixing device 10. The sample mixing device 10 may also include Figure 1 More or fewer components than shown, or with Figure 1 Different configurations shown. Figure 1 Each component shown in the figure can be implemented by hardware, software or a combination thereof.

[0065] In the present application, in order to ensure that the sample mixing device 10 can effectively quantitatively evaluate the specific mixing effect of the mixing operation of the sample to be tested and the corresponding reagent while avoiding meaningless loss of the sample to be tested and the corresponding reagent, so as to facilitate the test personnel to adjust the mixing operation details according to the sample to be tested and the preset reagent, so that the final test result is accurate and reliable, the embodiment of the present application provides a sample mixing simulation test method to achieve the above-mentioned purpose. The sample mixing simulation test method provided by the present application is described in detail below.

[0066] Please refer to Figure 2 , Figure 2 2 is a flow chart of a sample mixing simulation test method provided in an embodiment of the present application. In an embodiment of the present application, the sample mixing simulation test method may include steps S210 to S250.

[0067] Step S210 , obtaining a first absorbance of a simulated sample having a viscosity matching that of the sample to be tested at a first maximum absorption wavelength corresponding to a first fluorescent dye, wherein the simulated sample is obtained by dissolving the first fluorescent dye in a target viscous solvent.

[0068] In the present embodiment, the viscosity of the simulated sample is consistent with that of the sample to be tested, and the simulated sample is obtained by dissolving a first fluorescent material into a target viscous solvent, and the target viscous solvent is a solvent with high viscosity at a specific concentration. The first maximum absorption wavelength is used to represent the wavelength of light used by the first fluorescent dye under its own maximum absorbance. Wherein, the first fluorescent dye can be any one of dyes such as rhodamine B, methyl orange, methylene blue, gentian violet, etc. In one embodiment of the present embodiment, rhodamine B can be used as the first fluorescent dye, and a glycerol diluent is used as the target viscous solvent, then the simulated sample needs to be placed under the illumination of the maximum absorption wavelength (475nm) of rhodamine B, and the first absorbance of the simulated sample under the illumination of 475nm is measured to be 1.81.

[0069] Step S220 , obtaining a second absorbance of a simulation reagent matching a preset reagent viscosity at a second maximum absorption wavelength corresponding to a second fluorescent dye, wherein the simulation reagent is obtained by dissolving the second fluorescent dye in a target viscosity solvent.

[0070] In the present embodiment, the viscosity of the simulation reagent is consistent with that of the preset reagent, and the preset reagent is one or more reagents that need to be added to the sample to be tested when carrying out sample detection, and the simulation reagent adopts the second fluorescent material to be dissolved in the target viscous solvent to obtain, and the second fluorescent material is different from the first fluorescent material. The second maximum absorption wavelength is used to represent the wavelength of light used by the second fluorescent dye in the case of its maximum absorbance. Wherein, the second fluorescent dye can also be any one of the dyes such as rhodamine B, methyl orange, methylene blue, gentian violet, but the first fluorescent dye is different from the second fluorescent dye. In one embodiment of the present embodiment, orange red G pigment can be used as the second fluorescent dye, and glycerol diluent is used as the target viscous solvent, then simulation reagent needs to be placed under the illumination of the maximum absorption wavelength (554nm) of orange red G pigment, and measure the second absorbance 0.07 of the simulation reagent under the illumination of 554nm.

[0071] Step S230 , obtaining a third absorbance and a fourth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of the target viscous solvent, respectively.

[0072] In this embodiment, the target viscous solvent shared by the simulated reagent and the simulated sample can be exposed to light at the first maximum absorption wavelength of a first fluorescent dye, and the absorbance of the target viscous solvent under the first maximum absorption wavelength (i.e., a third absorbance) can be measured. Furthermore, the target viscous solvent can be exposed to light at the second maximum absorption wavelength of a second fluorescent dye, and the absorbance of the target viscous solvent under the second maximum absorption wavelength (i.e., a fourth absorbance) can be measured. In one embodiment of this embodiment, when a glycerol diluent is used as the target viscous solvent, the third absorbance of the target viscous solvent under illumination at the maximum absorption wavelength of rhodamine B (475 nm) is 0.02, and the fourth absorbance of the target viscous solvent under illumination at the maximum absorption wavelength of Tangerine G (554 nm) is 1.93.

[0073] Step S240 , obtaining a fifth absorbance and a sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, of a mixed reaction solution obtained by performing a sample-reagent mixing operation on the simulated sample and the simulated reagent.

[0074] In this embodiment, after determining a simulated sample that matches the viscosity of the sample to be tested and a simulated reagent that matches the viscosity of the preset reagent, the simulated sample can be used to replace the sample to be tested and the simulated reagent can be used to replace the preset reagent. A certain volume (V2) of the simulated sample and a certain volume (V1) of the simulated reagent are extracted and added to the automated sample detection device. The sample and reagent mixing operation is performed for a specific time according to the set mixing parameter conditions to obtain a corresponding mixed reaction liquid. Similarly, a certain volume (V2) of the simulated sample and a certain volume (V1) of the simulated reagent can also be extracted and added to the reaction container of the mixing component 14 of the sample mixing device 10. The sample mixing device 10 has the same hardware structure and parameter settings as the sample mixing module (such as an oscillation module) of the automated sample testing device, and can simulate the function of the sample mixing module of the automated sample testing device. The sample mixing device 10 performs a sample-reagent mixing operation for a specific mixing time in the reaction container according to the set mixing parameter conditions to obtain a corresponding mixed reaction liquid, so as to characterize the specific mixing effect of the sample to be tested and the preset reagent under the current sample-reagent mixing operation through the current mixed reaction liquid, thereby timely verifying the sample mixing performance of the automated sample testing device and ensuring that the sample detection results of the automated sample testing device are accurate and effective.

[0075] Furthermore, after obtaining the mixed reaction liquid processed by the current sample reagent mixing operation, the mixed reaction liquid can be placed under light of the first maximum absorption wavelength of the first fluorescent dye, and the absorbance of the mixed reaction liquid under the light of the first maximum absorption wavelength (i.e., the fifth absorbance) can be measured, and the mixed reaction liquid can be placed under light of the second maximum absorption wavelength of the second fluorescent dye, and the absorbance of the mixed reaction liquid under the light of the second maximum absorption wavelength (i.e., the sixth absorbance) can be measured.

[0076] During this process, it is worth noting that the sample and reagent mixing operation of the same execution step often results in different mixing effects depending on the mixing time. Taking glycerol diluent as the target viscous solvent, Rhodamine B as the first fluorescent dye, and Tangerine G pigment as the second fluorescent dye, the specific values ​​of the fifth absorbance and sixth absorbance of the mixed reaction solution corresponding to the sample and reagent mixing operation with different mixing times are as follows: Figure 3 As shown. Figure 3 As shown in the figure, the unit of time corresponding to the "mixing time" is 1 minute. When the mixing time is 8 minutes or more, the corresponding fifth absorbance and sixth absorbance will remain unchanged; when the mixing time gradually increases and is less than 8 minutes, the corresponding fifth absorbance will gradually decrease, and the corresponding sixth absorbance will gradually increase.

[0077] Step S250 , performing data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect evaluation parameter that matches the sample and reagent mixing operation.

[0078] In this embodiment, the calculation formula of the mixing effect evaluation parameter is as follows:

[0079]

[0080] Wherein, H is used to represent the mixing effect evaluation parameter of the mixed reaction liquid under the current sample and reagent mixing operation, A is used to represent the fifth absorbance of the mixed reaction liquid under the current sample and reagent mixing operation, B is used to represent the sixth absorbance of the mixed reaction liquid under the current sample and reagent mixing operation, A0 is used to represent the third absorbance of the target viscous solvent at the first maximum absorption wavelength, B0 is used to represent the fourth absorbance of the target viscous solvent at the second maximum absorption wavelength, A1 is used to represent the first absorbance of the simulated sample at the first maximum absorption wavelength, and B1 is used to represent the second absorbance of the simulated reagent at the second maximum absorption wavelength. The closer the value of the mixing effect evaluation parameter is to the volume ratio (V2 / V1) between the simulated sample and the simulated reagent, the better the mixing effect of the current sample and reagent mixing operation.

[0081] In this case, the sample mixing device 10 can perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance by calling the calculation formula of the above-mentioned mixing effect evaluation parameters to obtain mixing effect evaluation parameters that match the sample to be tested, the preset reagent and the current sample reagent mixing operation, thereby achieving an effective quantitative evaluation of the specific mixing effect of the mixing operation of the sample to be tested and the preset reagent, facilitating the inspection of the sample reagent mixing performance of the automated sample detection equipment, and also helping the detection personnel to adjust and optimize the operation details when manually performing the sample reagent mixing operation, so that the final detection result is accurate and reliable.

[0082] Therefore, the present application can execute the above-mentioned steps S210 to S250, and use the simulated samples and simulated reagents that actually participate in the mixing to replace the sample to be tested and the preset reagent respectively, so as to avoid meaningless loss of the sample to be tested and the preset reagent, and use the mixing effect evaluation parameters to effectively quantitatively evaluate the specific mixing effect of the mixing operation of the sample to be tested and the preset reagent, so as to facilitate the inspection of the sample reagent mixing performance of the automated sample detection equipment, and also help the detection personnel to adjust and optimize the operation details when manually performing the sample reagent mixing operation, so that the final detection result is accurate and reliable.

[0083] Alternatively, see Figure 4 , Figure 4 yes Figure 2 FIG2 is a flow chart of sub-steps included in step S250. In this embodiment, step S250 may include sub-steps S251 to S253.

[0084] In sub-step S251 , a first net absorption coefficient of the mixed reaction solution relative to the simulated sample in the sample-reagent mixing operation is calculated based on the first absorbance, the fifth absorbance, and the third absorbance.

[0085] In this embodiment, the first net absorbance coefficient is used to represent the absorbance multiple of the mixed reaction liquid relative to the simulated sample when excluding the absorbance interference of the target viscous solvent. In this case, the step of calculating the first net absorbance coefficient of the mixed reaction liquid relative to the simulated sample under the sample and reagent mixing operation based on the first absorbance, the fifth absorbance, and the third absorbance may include:

[0086] performing a subtraction operation on the fifth absorbance and the third absorbance to obtain a net absorbance of the mixed reaction solution at a first maximum absorption wavelength;

[0087] performing a subtraction operation on the first absorbance and the third absorbance to obtain a net absorbance of the simulated sample at a first maximum absorption wavelength;

[0088] A ratio operation is performed on the net absorbances of the mixed reaction solution and the simulated sample at the first maximum absorption wavelength to obtain the first net absorption coefficient.

[0089] At this time, the calculation formula of the first net absorption coefficient is the molecular expression in the calculation formula of the above-mentioned mixing effect evaluation parameter.

[0090] Sub-step S252 , calculating a second net absorption coefficient of the mixed reaction solution relative to the simulation reagent in the sample reagent mixing operation according to the second absorbance, the sixth absorbance, and the fourth absorbance.

[0091] In this embodiment, the second net absorbance coefficient is used to represent the absorbance multiple of the mixed reaction liquid relative to the simulation reagent when the absorbance interference of the target viscous solvent is excluded. In this case, the step of calculating the second net absorbance coefficient of the mixed reaction liquid relative to the simulation reagent under the sample reagent mixing operation based on the second absorbance, the sixth absorbance, and the fourth absorbance may include:

[0092] performing a subtraction operation on the sixth absorbance from the fourth absorbance to obtain a net absorbance of the mixed reaction solution at a second maximum absorption wavelength;

[0093] performing a subtraction operation on the second absorbance and the fourth absorbance to obtain a net absorbance of the simulation reagent at a second maximum absorption wavelength;

[0094] A ratio operation is performed on the net absorbances of the mixed reaction solution and the simulation reagent at the second maximum absorption wavelength to obtain the second net absorption coefficient.

[0095] At this time, the calculation formula of the second net light absorption coefficient is the denominator expression in the calculation formula of the above-mentioned mixing effect evaluation parameter.

[0096] Sub-step S253 , performing a ratio operation on the first net absorption coefficient and the second net absorption coefficient to obtain a mixing effect evaluation parameter that matches the sample reagent mixing operation.

[0097] In this embodiment, after the sample mixing device 10 calculates the first net absorption coefficient and the second net absorption coefficient corresponding to the mixed reaction liquid of the current sample reagent mixing operation, the mixing effect evaluation parameter corresponding to the current sample reagent mixing operation can be obtained by performing a ratio operation on the first net absorption coefficient and the second net absorption coefficient.

[0098] In this process, it is worth noting that the sample and reagent mixing operations of the same execution steps often result in different mixing effect evaluation parameters as the mixing time varies. Glycerol diluent is used as the target viscous solvent, Rhodamine B is used as the first fluorescent dye, and Tangerine G pigment is used as the second fluorescent dye, and Figure 3 The fifth and sixth absorbances under the sample-reagent mixing operation with different mixing times are shown. At this time, the specific values ​​of the mixing effect evaluation parameters corresponding to the simulated sample and the simulated reagent under the sample-reagent mixing operation with different mixing times are as follows: Figure 5 As shown. Figure 5 As shown in the figure, the unit of time corresponding to "Mixing time" is 1 minute. When the mixing time is 8 minutes or above, the corresponding mixing effect evaluation parameter will remain unchanged, and the corresponding mixing effect is the best at this time. When the mixing time gradually increases and is less than 8 minutes, the corresponding mixing effect evaluation parameter will gradually decrease, and the corresponding mixing effect gradually improves.

[0099] Therefore, the present application can effectively and quantitatively evaluate the specific mixing effects of the sample to be tested and the preset reagent under the current sample reagent mixing operation by executing the above-mentioned sub-steps S251 to S253, which is convenient for testing the sample reagent mixing performance of the automated sample detection equipment, and also helps the detection personnel to adjust and optimize the operation details when manually performing the sample reagent mixing operation, so that the final detection results are accurate and reliable.

[0100] In this application, to ensure that the sample mixing device 10 can perform the above-mentioned sample mixing simulation test method through the sample mixing simulation test device 100, this application implements the aforementioned functions by dividing the sample mixing simulation test device 100 into functional modules. The specific components of the sample mixing simulation test device 100 provided in this application are described below.

[0101] Please refer to Figure 6 , Figure 6 Schematic diagram of the components of a sample mixing simulation test device 100 provided in an embodiment of the present application. In this embodiment of the present application, the sample mixing simulation test device 100 may include a simulated sample absorbance acquisition module 110, a simulated reagent absorbance acquisition module 120, a viscous solvent absorbance acquisition module 130, a mixing operation absorbance acquisition module 140, and a mixing effect evaluation and analysis module 150.

[0102] The simulated sample absorbance acquisition module 110 is used to acquire a first absorbance of a simulated sample having a viscosity matching that of the sample to be tested at a first maximum absorption wavelength corresponding to a first fluorescent dye, wherein the simulated sample is obtained by dissolving the first fluorescent dye in a target viscosity solvent.

[0103] The simulation reagent absorbance acquisition module 120 is used to obtain the second absorbance of the simulation reagent matching the preset reagent viscosity at the second maximum absorption wavelength corresponding to the second fluorescent dye, wherein the simulation reagent is obtained by dissolving the second fluorescent dye in the target viscosity solvent.

[0104] The viscous solvent absorption acquisition module 130 is configured to acquire a third absorbance and a fourth absorbance of the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively.

[0105] The mixing operation absorbance acquisition module 140 is used to obtain a fifth absorbance and a sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, of a mixed reaction solution obtained by performing a sample-reagent mixing operation on the simulated sample and the simulated reagent.

[0106] The mixing effect evaluation and analysis module 150 is configured to perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect evaluation parameter that matches the sample-reagent mixing operation. The closer the mixing effect evaluation parameter is to the volume ratio of the simulated sample to the simulated reagent corresponding to the mixed reaction solution, the better the mixing effect of the sample-reagent mixing operation.

[0107] Alternatively, see Figure 7 , Figure 7 yes Figure 6 Schematic diagram of the composition of the mixing effect evaluation and analysis module 150 in FIG. In this embodiment, the mixing effect evaluation and analysis module 150 may include a first net light absorption calculation submodule 151, a second net light absorption calculation submodule 152 and a mixing effect evaluation submodule 153.

[0108] The first net absorbance calculation submodule 151 is configured to calculate a first net absorbance coefficient of the mixed reaction solution relative to the simulated sample during the sample-reagent mixing operation based on the first absorbance, the fifth absorbance, and the third absorbance.

[0109] The second net absorbance calculation submodule 152 is configured to calculate a second net absorbance coefficient of the mixed reaction solution relative to the simulation reagent during the sample reagent mixing operation based on the second absorbance, the sixth absorbance, and the fourth absorbance.

[0110] The mixing effect evaluation submodule 153 is configured to perform a ratio operation on the first net light absorption coefficient and the second net light absorption coefficient to obtain a mixing effect evaluation parameter that matches the sample reagent mixing operation.

[0111] It should be noted that the basic principles and technical effects of the sample mixing simulation test device 100 provided in the present embodiment are the same as those of the aforementioned sample mixing simulation test method. For the sake of brevity, any details not mentioned in this embodiment can be referred to the description of the aforementioned sample mixing simulation test method.

[0112] In the embodiments provided in this application, it should be understood that the disclosed devices and methods can also be implemented in other ways. The device embodiments described above are merely schematic. For example, the flowcharts and block diagrams in the accompanying drawings show the possible architectures, functions and operations of the devices, methods and computer program products according to the embodiments of the present application. In this regard, each box in the flowchart or block diagram can represent a module, a program segment or a part of a code, and the module, program segment or a part of the code contains one or more executable instructions for implementing the specified logical functions. It should also be noted that in some alternative implementations, the functions marked in the box can also occur in an order different from that marked in the accompanying drawings. For example, two consecutive boxes can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, depending on the functions involved. It should also be noted that each box in the block diagram and / or flowchart, and the combination of boxes in the block diagram and / or flowchart, can be implemented using a dedicated hardware-based system that performs the specified function or action, or can be implemented using a combination of dedicated hardware and computer instructions.

[0113] In addition, the functional modules in each embodiment of the present application can be integrated together to form an independent part, or each module can exist separately, or two or more modules can be integrated to form an independent part. If the function is implemented in the form of a software functional module and sold or used as an independent product, it can be stored in a storage medium. Based on this understanding, the technical solution of the present application is essentially or the part that contributes to the prior art or the part of the technical solution can be embodied in the form of a software product, which is stored in a readable storage medium and includes several instructions for enabling a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the method described in each embodiment of the present application. The aforementioned storage medium includes various media that can store program codes, such as a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk or an optical disk.

[0114] In summary, in the sample mixing simulation test method and apparatus, sample mixing equipment and storage medium provided in the present application, the present application obtains the first absorbance of the simulated sample that matches the viscosity of the sample to be tested at the first maximum absorption wavelength of the autofluorescent material, the second absorbance of the simulated reagent that matches the viscosity of the preset reagent at the second maximum absorption wavelength of the autofluorescent material, the third absorbance and fourth absorbance of the target viscosity solvent shared by the simulated reagent and the simulated sample at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, and the fifth absorbance of the mixed reaction liquid after the simulated sample and the simulated reagent are mixed by the sample reagent operation at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively. and the sixth absorbance, and then perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance to obtain mixing effect evaluation parameters that match the sample reagent mixing operation, so that the simulated sample and the simulated reagent that actually participate in the mixing are used to replace the sample to be tested and the preset reagent respectively, so as to avoid meaningless loss of the sample to be tested and the preset reagent, and effectively quantitatively evaluate the specific mixing effect of the mixing operation of the sample to be tested and the preset reagent through the mixing effect evaluation parameters, which is convenient for testing the sample reagent mixing performance of the automated sample detection equipment, and also helps the detection personnel to adjust and optimize the operation details when manually performing the sample reagent mixing operation, so that the final detection result is accurate and reliable.

[0115] The above are merely various embodiments of the present application, but the scope of protection of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

Claims

1. A sample mixing simulation test method, characterized in that: The method comprises: Obtaining a first absorbance of a simulated sample having a viscosity matching that of the sample to be tested at a first maximum absorption wavelength corresponding to the first fluorescent dye, wherein the simulated sample is obtained by dissolving the first fluorescent dye in a target viscosity solvent; Obtaining a second absorbance of a simulation reagent matching a preset reagent viscosity at a second maximum absorption wavelength corresponding to a second fluorescent dye, wherein the simulation reagent is obtained by dissolving the second fluorescent dye in the target viscosity solvent; Obtaining a third absorbance and a fourth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of the target viscous solvent, respectively; Obtaining a fifth absorbance and a sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, of a mixed reaction solution obtained by performing a sample-reagent mixing operation on the simulated sample and the simulated reagent; Data analysis is performed on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect evaluation parameter that matches the sample-reagent mixing operation, wherein the mixing effect evaluation parameter is used to indicate the quality of the specific mixing effect of the corresponding sample and the corresponding reagent under the current mixing operation; the calculation formula of the mixing effect evaluation parameter is as follows: ； in, H It is used to represent the mixing effect evaluation parameter of the mixed reaction solution under the current sample and reagent mixing operation. A It is used to indicate the fifth absorbance of the mixed reaction solution under the current sample and reagent mixing operation. B It is used to indicate the sixth absorbance of the mixed reaction solution under the current sample and reagent mixing operation. A 0 is used to represent the third absorbance of the target viscous solvent at the first maximum absorption wavelength, B 0 is used to represent the fourth absorbance of the target viscous solvent at the second maximum absorption wavelength, A 1 is used to represent the first absorbance of the simulated sample at the first maximum absorption wavelength, B 1 is used to represent the second absorbance of the simulation reagent at the second maximum absorption wavelength.

2. The method according to claim 1, characterized in that The closer the mixing effect evaluation parameter is to the volume ratio of the simulated sample and the simulated reagent corresponding to the mixed reaction solution, the better the mixing effect of the sample-reagent mixing operation.

3. A sample mixing simulation test device, characterized in that: The device comprises: a simulated sample absorbance acquisition module, configured to acquire a first absorbance of a simulated sample having a viscosity matching that of the sample to be tested at a first maximum absorption wavelength corresponding to a first fluorescent dye, wherein the simulated sample is obtained by dissolving the first fluorescent dye in a target viscosity solvent; a simulation reagent absorbance acquisition module, configured to acquire a second absorbance of a simulation reagent matching a preset reagent viscosity at a second maximum absorption wavelength corresponding to a second fluorescent dye, wherein the simulation reagent is obtained by dissolving the second fluorescent dye in the target viscosity solvent; a viscous solvent absorption acquisition module, configured to acquire a third absorbance and a fourth absorbance of the target viscous solvent corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength, respectively; a mixing operation absorbance acquisition module, configured to acquire a fifth absorbance and a sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, of a mixed reaction solution obtained by performing a sample-reagent mixing operation on the simulated sample and the simulated reagent; A mixing effect evaluation and analysis module is configured to perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect evaluation parameter that matches the sample-reagent mixing operation, wherein the mixing effect evaluation parameter is used to indicate the quality of the specific mixing effect of the corresponding sample and the corresponding reagent under the current mixing operation; the calculation formula of the mixing effect evaluation parameter is as follows: ； in, H It is used to represent the mixing effect evaluation parameter of the mixed reaction solution under the current sample and reagent mixing operation. A It is used to indicate the fifth absorbance of the mixed reaction solution under the current sample and reagent mixing operation. B It is used to indicate the sixth absorbance of the mixed reaction solution under the current sample and reagent mixing operation. A 0 is used to represent the third absorbance of the target viscous solvent at the first maximum absorption wavelength, B 0 is used to represent the fourth absorbance of the target viscous solvent at the second maximum absorption wavelength, A 1 is used to represent the first absorbance of the simulated sample at the first maximum absorption wavelength, B 1 is used to represent the second absorbance of the simulation reagent at the second maximum absorption wavelength.

4. The device according to claim 3, characterized in that The closer the mixing effect evaluation parameter is to the volume ratio of the simulated sample and the simulated reagent corresponding to the mixed reaction solution, the better the mixing effect of the sample-reagent mixing operation.

5. A sample mixing device, characterized in that: It comprises a processor and a memory, wherein the memory stores a computer program that can be executed by the processor, and the processor can execute the computer program to implement the sample mixing simulation test method according to claim 1 or 2.

6. A storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by a processor, the sample mixing simulation test method according to claim 1 or 2 is implemented.

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