A sample collection mixing device for PCR detection

Abstract

The application discloses a sample collection and mixing device for PCR detection, which comprises a collection component, a mixing component and a display component, one end of the mixing component is connected with the collection component, and the other end of the mixing component is connected with the display component; the collection component is sleeved with the end of the mixing component, and the collection component is movable on the mixing component. The collection component, the mixing component and the display component are arranged, so that the device has the functions of collection and mixing, and is fully sealed during the collection and mixing process. After collection, the device is automatically discharged into a PCR transparent tube through pressure relief, so that workers only need to place the device into professional detection equipment, the to-be-detected liquid is fully sealed during the collection and mixing process, the safety of liquid collection is improved, the spread of viruses is effectively avoided, the labor intensity of workers is reduced, and the detection efficiency is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of liquid collection portable devices, and particularly relates to a sample collection mixed solution device for PCR detection. BACKGROUND

[0002] The fluorescent quantitative PCR technology is a method for monitoring the whole PCR process in real time by adding a fluorescent group in the PCR reaction system and utilizing the fluorescent signal accumulation, and finally quantitatively analyzing the unknown template through a standard curve.

[0003] Before the fluorescent quantitative PCR detection, a liquid sample needs to be collected, then the liquid sample is mixed with a reagent to form a mixed solution, and finally the mixed solution is taken to a designated device for detection, usually optical instruments for optical detection to obtain a detection result. The liquid sample can usually include saliva (nucleic acid detection), blood (liver function and blood routine detection), and urine (prostate and kidney function detection), which are common liquid samples in common medical detection items.

[0004] At present, the liquid sample detection process usually involves that a worker issues a test tube to a user to collect a specified sample, after the collection, the worker injects the test tube with the liquid sample into a reagent to obtain a mixed solution, and in the whole process, the test tube needs to be frequently opened and mixed, and when facing a large number of liquid mixing work, the detection efficiency will be reduced, at the same time, opening the test tube will cause the internal liquid sample to be in contact with air, which affects the detection accuracy, and the peculiar smell of the liquid sample will also affect the working state of the worker. SUMMARY

[0005] The application provides a sample collection mixed solution device for PCR detection, and aims to solve the problem of low detection efficiency caused by the sample collection and mixing process in the existing PCR detection.

[0006] According to the application, a sample collection mixed solution device for PCR detection is provided, which comprises a collection assembly, a mixing assembly and a developing assembly, one end of the mixing assembly is connected with the collection assembly, the other end of the mixing assembly is connected with the developing assembly, the collection assembly is sleeved on the end of the mixing assembly, and the collection assembly moves on the mixing assembly, the mixing assembly and the developing assembly are in communication, the mixing assembly comprises a communication part, a sliding part and a liquid outlet part which are sequentially communicated, the liquid outlet part is in communication with the developing assembly, the sliding part is provided with a sliding plunger and an elastic element, one end of the elastic element abuts against the sliding part, and the other end of the elastic element abuts against the plunger, a through hole is formed in the plunger, one end of the plunger is buckled on the sliding part, when the collection assembly moves close to the sliding part, the collection assembly abuts against the plunger, the plunger is separated from the sliding part, and the through hole enters the communication part.

[0007] Preferably, the plunger comprises a moving plug and a movable valve, the moving plug is arranged to slide in the sliding part, and the movable valve is connected to one end of the moving plug; the movable valve is connected to one end of the sliding part away from the moving plug, and the movable valve is provided with a pressing point at the connected end, and the pressing point is arranged to correspond to the collection assembly.

[0008] Preferably, the collection assembly comprises a collection cup, a flip cover and a push rod, the flip cover is connected to the collection cup, and the push rod is arranged on the flip cover; the collection cup is threadedly connected to the communication part, and the collection cup is arranged to correspond to the pressing point; the collection cup is provided with a film breaking cylinder, the film breaking cylinder is a cylindrical structure extending towards the mixing assembly, and when the flip cover is closed on the collection cup, the push rod is inserted into the film breaking cylinder.

[0009] Preferably, the communication part is provided with a first film layer and a second film layer, and the communication part, the first film layer and the second film layer enclose a space for accommodating reagents; when the flip cover is closed on the collection cup, the push rod is located in the region of the first film layer.

[0010] Preferably, the moving plug is provided with a protrusion at one end away from the movable valve, and when the through hole moves to the region of the communication part, the protrusion abuts against the sliding part.

[0011] Preferably, the movable valve comprises a bent rod, the pressing point is arranged at the end of the bent rod, and the pressing point abuts against the sliding part; the sliding part is provided with a sliding groove at a position corresponding to the pressing point, the end of the sliding groove is provided with a broken part, and the pressing point passes through the broken part to abut against the side surface of the sliding groove.

[0012] Preferably, the developing assembly is a PCR transparent tube.

[0013] Preferably, the collection cup is provided with a sealing ring at a region close to the communication part, and the push rod is provided with a silica gel block at an end away from the flip cover, and the silica gel block is interference-fitted with the sealing ring.

[0014] Compared with the prior art, the sample collection and mixing device for PCR detection provided by the present application has the following beneficial effects:

[0015] By arranging the collection assembly, the mixing assembly and the developing assembly, the device collects and mixes the sample, realizes full sealing during the collection and mixing, and automatically discharges into the PCR transparent tube through pressure relief after collection, so that the worker only needs to place the whole device into a professional detection equipment, which can realize full sealing of the sample during the collection and mixing, improve the safety of sample collection, effectively avoid the spread of viruses, reduce the labor intensity of the worker and improve the detection efficiency. Meanwhile, the device has a simple and light structure and is suitable for carrying. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 is a schematic diagram of a sample collection and mixing device for PCR detection provided in the first embodiment of the present invention.

[0018] Figure 2 is a schematic diagram of the structure of a sample collection and mixing device for PCR detection with the flip-top open, provided in the first embodiment of the present invention.

[0019] Figure 3 is a cross-sectional view of a PCR detection sample collection and mixing device provided in the first embodiment of the present invention when the lid is opened.

[0020] Figure 4 is a schematic diagram of the plunger component in a PCR detection sample collection and mixing device provided in the first embodiment of the present invention.

[0021] Figure 5 This is a cross-sectional view of a PCR detection sample collection and mixing device provided in the first embodiment of the present invention in the closed state.

[0022] Figure 6 is a cross-sectional view of a PCR detection sample collection and mixing device provided in the first embodiment of the present invention when the first membrane layer is punctured.

[0023] Figure 7 This is a cross-sectional view of a PCR detection sample collection and mixing device provided in the first embodiment of the present invention when the second membrane layer is punctured.

[0024] Figure 8 is a cross-sectional view of a PCR detection sample collection and mixing device provided in the first embodiment of the present invention when the plunger is disengaged.

[0025] Explanation of reference numerals in the attached figures:

[0026] 1. Acquisition component; 11. Acquisition cup; 12. Flip cover; 13. Push rod; 14. Membrane breaking cylinder;

[0027] 2. Mixing assembly; 21. Connecting part; 211. First membrane layer; 212. Second membrane layer; 22. Sliding part; 221. Plunger component; 2211. Moving plug; 2212. Movable valve; 2213. Pressing point; 2214. Protrusion; 2215. Bending rod; 2216. Slide groove; 222. Elastic element; 223. Through hole; 23. Liquid outlet;

[0028] 3. The imaging assembly. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of the present application.

[0030] It should also be understood that the terms used in the specification of the present application are only for the purpose of describing specific embodiments and are not intended to limit the present application. As used in the specification and the appended claims of the present application, the singular forms "a", "an" and "the" are intended to include the plural forms unless the context clearly indicates otherwise.

[0031] It should be further understood that the term "and / or" used in the specification and the appended claims of the present application means any combination of one or more of the associated listed items and all possible combinations thereof.

[0032] Please refer to Figure 1 , Figure 2 and Figure 3 , the first embodiment of the present application discloses a sample collection and mixing device for PCR detection, comprising a collection assembly 1, a mixing assembly 2 and an imaging assembly 3, one end of the mixing assembly 2 is connected to the collection assembly 1, the other end of the mixing assembly 2 is connected to the imaging assembly 3, the collection assembly 1 is used to obtain saliva of a testee, the mixing assembly 2 is used to receive saliva in the collection assembly 1 and mix with the built-in detection reagent, the imaging assembly 3 is used to receive the mixed liquid formed in the mixing assembly 2 and give detection instrument through a transparent shell for detection to obtain a detection result.

[0033] It can be understood that the sample collection and mixing device for PCR detection can be used to collect saliva, blood or urine and other liquids to be detected. In this embodiment, saliva collection for nucleic acid detection is taken as an example for description, which is not intended to limit the present solution.

[0034] The collection assembly 1 is sleeved on the end of the mixing assembly 2, and the collection assembly 1 is moved on the mixing assembly 2. The mixing assembly 2 is in communication with the imaging assembly 3, the mixing assembly 2 comprises a communication part 21, a sliding part 22 and a liquid outlet part 23 which are sequentially communicated, the liquid outlet part 23 is in communication with the imaging assembly 3, the communication part 21 is in communication with the collection assembly 1, and saliva in the collection assembly 1 flows into the communication part 21.

[0035] Please refer toFigure 3 and Figure 4 The sliding part 22 is provided with a plunger 221 and an elastic member 222. One end of the elastic member 222 is abutted against the sliding part 22, and the other end is abutted against the plunger 221. When the plunger 221 is separated from the sliding part 22, the elastic member 222 drives the plunger 221 to move based on the elastic force. The plunger 221 is provided with a through hole 223. The movement of the plunger 221 adjusts the relative position of the through hole 223 relative to the communication part 21. When the through hole 223 moves to the area of the communication part 21, the communication part 21 is in communication with the liquid outlet part 23.

[0036] One end of the plunger 221 is buckled to the sliding part 22. When the collection assembly 1 moves close to the sliding part 22, the collection assembly 1 abuts against the plunger 221, and the plunger 221 is separated from the sliding part 22. The through hole 223 enters the communication part 21.

[0037] It can be understood that the plunger 221 is partially exposed to the sliding part 22, so as to abut against the sliding part 22 through the exposed part. When the collection assembly 1 moves close to the sliding part 22, the exposed part of the plunger 221 in the area is abutted and pressed, so that the plunger 221 is separated from the abutting state of the sliding part 22. Under the action of the elastic member 222, the plunger 221 slides in the sliding part 22. When the elastic force of the elastic member 222 completely disappears, the through hole 223 enters the range of the communication part 21, so that the communication part 21, the through hole 223 and the liquid outlet part 23 are in communication. The mixed liquid in the communication part 21 can flow into the developing assembly 3.

[0038] Specifically, as shown in Figure 4 The plunger 221 includes a moving plug 2211 and a movable valve 2212. The moving plug 2211 is arranged to slide in the sliding part 22, and the movable valve 2212 is inserted and positioned at one end of the moving plug 2211.

[0039] One end of the movable valve 2212 buckled to the sliding part 22 away from the moving plug 2211, and the movable valve 2212 is provided with a pressing point 2213 at the buckled end. The pressing point 2213 is arranged corresponding to the collection assembly 1.

[0040] It can be understood that one end of the elastic member 222 abuts against the movable valve 2212, and the other end abuts against the sliding part 22. The elastic member 222 pushes the movable valve 2212 to slide through the elastic force. That is, when the pressing point 2213 on the movable valve 2212 is buckled to the sliding part 22, the elastic member 222 is in a pressed state (as shown in Figure 3When the collection assembly 1 is moved downward to press the pressing point 2213, the movable valve 2212 can be separated from the buckling point on the sliding part 22 (as shown in the disengaged state), and slide based on the elastic force. Figure 8

[0041] Please continue to refer to Figure 4 The movable valve 2212 includes a bent rod 2215, and the pressing point 2213 is arranged at an end of the bent rod 2215 and abuts against the sliding part 22. The sliding part 22 is provided with a sliding groove 2216 corresponding to the position of the pressing point 2213, and an end of the sliding groove 2216 is provided with a broken part. The pressing point 2213 passes through the broken part and abuts against the side surface of the sliding groove 2216. When the pressing point 2213 is pressed, the bent rod 2215 is forced to bend, the pressing point 2213 is separated from the sliding groove 2216 and enters the broken part. That is, one end of the bent rod 2215 is fixed and the other end is movable. When the pressing point 2213 is pressed, the movable end of the bent rod 2215 is pressed and bent.

[0042] Please continue to refer to Figure 3 and Figure 4 One end of the moving plug 2211 away from the movable valve 2212 is provided with a protrusion 2214. When the through hole 223 moves to the region of the communication part 21, the protrusion 2214 abuts against the sliding part 22, so that the moving plug 2211 is prevented from moving out of the sliding part 22 too much. At the same time, the limiting effect of the protrusion 2214 can ensure that the through hole 223 is located in the region of the communication part 21 when the moving plug 2211 is slid to the position.

[0043] It can be understood that the moving plug 2211 is provided with a sealing ring, and the sealing ring is arranged on both sides of the through hole 223, and / or a sealing ring is arranged on the movable valve 2212 and arranged between the moving plug 2211 and the movable valve 2212.

[0044] Please continue to refer to Figure 2 and Figure 5 The collection assembly 1 includes a collection cup 11, a flip cover 12 and a push rod 13. The flip cover 12 is connected with the collection cup 11, and the push rod 13 is arranged on the flip cover 12. The collection cup 11 is threadedly connected with the communication part 21, and the outer circumferential side of the collection cup 11 is arranged in correspondence with the pressing point 2213. That is, the collection cup 11 slides on the communication part 21 in a threaded rotation mode, and other modes can also be arranged, for example, a straight sliding mode of a sliding groove.

[0045] ​The collecting cup 11 is provided with a liquid gathering opening, and a membrane breaking cylinder 14 is formed on the outer periphery of the liquid gathering opening and extends towards the mixing assembly 2. When the cover 12 is closed on the collecting cup 11, the push rod 13 is inserted into the membrane breaking cylinder 14, and the push rod 13 is provided with a rubber ring on the outer periphery. The push rod 13 is in interference fit with the membrane breaking cylinder 14.

[0046] The collecting cup 11 is rotated to ascend or descend on the communicating part 21, and the collecting cup 11 is moved to abut on the pressing point 2213, and the buckling state of the pressing point 2213 is controlled.

[0047] Please continue to combine Figure 2 and Figure 5 The communicating part 21 is provided with a first membrane layer 211 and a second membrane layer 212, and the communicating part 21, the first membrane layer 211 and the second membrane layer 212 enclose a space for accommodating a reagent.

[0048] When the cover 12 is closed on the collecting cup 11, the push rod 13 pushes the saliva into the membrane breaking cylinder 14, and at this time, the saliva is located in a cavity a formed between the membrane breaking cylinder 14, the first membrane layer 211 and the push rod 13 (as shown in the closed state of Figure 5 At this time, the user drives the collecting cup 11 to move on the communicating part 21, so that the membrane breaking cylinder 14 moves towards the first membrane layer 211 (as shown in the c direction of Figure 6 ), until the membrane breaking cylinder 14 pierces the first membrane layer 211, the saliva enters above the second membrane layer 212 from the first membrane layer 211, and the saliva is mixed with the reagent in the space b (as shown in Figure 6 ). When the user continues to move the collecting cup 11, so that the membrane breaking cylinder 14 continues to move downward, the membrane breaking cylinder 14 pierces the second membrane layer 212 (as shown in Figure 7 ), and the mixed liquid continues to fall downward to enter into the sliding part 22. At this time, the end of the collecting cup 11 is in contact with the pressing point 2213 (as shown in the state of Figure 7 ). Continue to move the collecting cup 11 so that the pressing point 2213 is separated from the sliding groove 2216, so that the plunger 221 moves (as shown in the state of Figure 8 ), and the through hole 223 enters the area of the communicating part 21.

[0049] It can be understood that the first membrane layer 211 and the second membrane layer 212 are PET, AL, PE or other easy-to-tear sealing materials, and the membrane breaking cylinder 14 can easily pierce the membrane layer under the pressing of the user.

[0050] Optionally, as an embodiment, the collection cup 11 is provided with a sealing ring near the area close to the communication part 21, and the push rod 13 is provided with a silica gel block away from the end of the flip cover, and the silica gel block is in interference fit with the sealing ring. By providing the silica gel block, the saliva collected at the bottom of the collection cup 11 is completely squeezed out through the cooperation of the silica gel block and the sealing ring, ensuring the dosage of the saliva and the reagent reaction.

[0051] It can be understood that the imaging assembly 3 is a PCR transparent tube, and after the collected liquid to be tested and the reagent are mixed, the imaging assembly 3 is used to be placed into a professional optical detection device for detection. For example, in the embodiment, the detection device is a device for nucleic acid detection, and the saliva of the person to be tested is mixed with the preservative liquid or the lysis reagent in the communication part 21.

[0052] It can be understood that when the user collects saliva from the collection cup 11, the movable valve 2212 is buckled on the sliding groove 2216, at this time the moving plug 2211 blocks and separates the communication part 21 from the liquid outlet part 23, the user presses the flip cover 12 to make the saliva fall into the communication part 21, and at the same time the collection cup 11 is continuously lowered, the edge of the collection cup 11 is pressed to the pressing point 2213, so that the movable valve 2212 moves to the d direction (as shown in Figure 8 When the membrane breaking cylinder 14 successively pokes the first membrane layer 211 and the second membrane layer 212 downward, the push rod 13 is continuously pressed downward, a pressure is formed in the communication part 21, so that when the through hole 223 and the communication part 21 are communicated, a pressure relief effect is formed, and the mixed liquid quickly enters the imaging assembly 3.

[0053] Compared with the prior art, the sample collection and mixed liquid device for PCR detection provided by the present application has the following beneficial effects:

[0054] By arranging the collection assembly, the mixed liquid assembly and the imaging assembly, the device has the functions of collection and mixing, and realizes full sealing during the collection and mixing process. After collection, the device is automatically discharged into the PCR transparent tube through the pressure relief effect, so that the worker only needs to place the whole device into a professional detection equipment, the liquid to be tested can be fully sealed during the collection and mixing process, the safety of liquid collection is improved, the spread of virus is effectively avoided, the labor intensity of the worker is reduced, and the detection efficiency is improved. At the same time, the equipment has simple structure and is light and portable.

[0055] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of various equivalent modifications or replacements within the technical range disclosed by the present application, and these modifications or replacements should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A sample collection reagent device for PCR detection, characterized by It comprises a collecting component, a mixing component and a developing component, one end of the mixing component is connected with the collecting component, and the other end of the mixing component is connected with the developing component; The collecting component is sleeved on the end of the mixing component, and the collecting component moves on the mixing component; The mixing component is communicated with the developing component, and the mixing component comprises a communicating part, a sliding part and a liquid outlet part which are communicated in sequence, and the liquid outlet part is communicated with the developing component; The sliding part is provided with a sliding plunger and an elastic element, one end of the elastic element abuts against the sliding part, and the other end abuts against the plunger; A through hole is formed in the plunger; One end of the plunger is buckled on the sliding part, when the collecting component moves close to the sliding part, the collecting component abuts against the plunger, the plunger is separated from the sliding part, and the through hole enters the communicating part; The plunger comprises a moving plug and a movable valve, the moving plug is arranged in the sliding part and slides, and the movable valve is inserted and positioned at one end of the moving plug; One end of the movable valve away from the moving plug is buckled on the sliding part, and the movable valve is provided with a pressing point at the buckled end, and the pressing point is correspondingly arranged with the collecting component; The collecting component comprises a collecting cup, a flip cover and a push rod, the flip cover is connected with the collecting cup, and the push rod is arranged on the flip cover; The collecting cup is threadedly connected with the communicating part, and the collecting cup is correspondingly arranged with the pressing point; The collecting cup is provided with a film breaking cylinder, the film breaking cylinder is a cylindrical structure extending towards the mixing component, when the flip cover is covered on the collecting cup, the push rod is inserted in the film breaking cylinder; The communicating part is provided with a first film layer and a second film layer, and the communicating part, the first film layer and the second film layer form a space for accommodating reagent; When the flip cover is covered on the collecting cup, the film breaking cylinder is located in the region of the first film layer; Wherein, one end of the moving plug away from the movable valve is provided with a protrusion, when the through hole moves to the region of the communicating part, the protrusion abuts against the sliding part; The movable valve comprises a bent rod, the pressing point is arranged at the end of the bent rod, and the pressing point abuts against the sliding part; The sliding part is provided with a sliding groove corresponding to the position of the pressing point, the end of the sliding groove is provided with a broken part, and the pressing point passes through the broken part and abuts against the side surface of the sliding groove.

2. The sample collection reagent device for PCR detection according to claim 1, wherein: The developing component is a PCR transparent tube.

3. The sample collection reagent device for PCR detection according to claim 1, wherein: The region of the collecting cup close to the communicating part is provided with a sealing ring, one end of the push rod away from the flip cover is provided with a silica gel block, and the silica gel block is interference fit with the sealing ring.

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