A chemiluminescent imaging device for multi-region shower samples

Abstract

A multi-region chemiluminescence imaging device belongs to the field of chemiluminescence visualization technology. Its purpose is to solve the problems of uneven manual leaching, long leaching time, and the inability of existing leaching systems to simultaneously leach and image multiple imprinted membrane samples. The invention includes: a support frame; a sample stage unit disposed within the support frame, the sample stage unit including at least a sample stage and a position adjustment structure that adjusts the sample stage relative to the support frame along the X and Z directions; a colorimetric solution spraying unit disposed above the sample stage, the colorimetric solution spraying unit including at least a nozzle, different colorimetric solutions being delivered to the nozzle and sprayed according to a set mixing ratio; a nozzle motion control unit, adjusting and controlling the movement of the nozzle in the Y and Z directions; a temperature control unit, the temperature control unit including at least a temperature control chamber, the colorimetric solution bottle being located within the temperature control chamber; and an imaging and image acquisition unit disposed above the sample stage unit, acquiring images of multiple imprinted membranes to be tested disposed on the sample stage.

Description

Technical Field

[0001] The invention belongs to the technical field of chemiluminescence visualization, and in particular relates to a chemiluminescence imaging device for multi-region shower samples. Background Art

[0002] Chemiluminescent immunoblotting can be used to detect target proteins, qualitatively or quantitatively determine protein expression, protein-gene interactions, etc. The main principle is to use substrate color development to detect the protein components expressed by specific target genes separated by electrophoresis.

[0003] In the prior art, chemiluminescent immunoblotting generally adopts manual elution or elution with the aid of an elution system. For manual elution, since the immunoblotting chemiluminescent reagent has a strong luminescent signal but a short luminescence time, manual elution has problems such as uneven elution and long elution time. For the elution system, the existing elution system is separated from the imaging system, and it is impossible to quickly elute and image multiple blotting membrane samples of different shapes, which affects the quality and speed of chemiluminescent detection. Summary of the invention

[0004] The purpose of the present invention is to provide a multi-region chemiluminescent imaging device to solve the problems of uneven manual sparging, long sparging time and inability of the sparging system to achieve simultaneous sparging and imaging of multiple blotting membrane samples in the prior art.

[0005] To achieve the above object, a multi-region chemiluminescent imaging device of the present invention comprises:

[0006] Support frame;

[0007] A sample stage unit is arranged in the support frame, and the sample stage unit at least comprises a sample stage and a position adjustment structure that drives the sample stage to adjust relative to the support frame along the X direction and the Z direction;

[0008] A color developing liquid spraying unit is arranged above the sample stage, and the color developing liquid spraying unit at least comprises a nozzle, a chemical color developing liquid bottle I, a chemical color developing liquid bottle II and a fluid delivery control structure, and the different color developing liquids in the chemical color developing liquid bottle I and the chemical color developing liquid bottle II are delivered to the nozzle and sprayed according to a set mixing ratio through the fluid delivery control structure;

[0009] A nozzle motion control unit, through which the nozzle motion is adjusted and controlled in Y and Z directions;

[0010] A constant temperature unit, the constant temperature unit at least comprising a constant temperature chamber, wherein chemical color developing liquid bottle I and chemical color developing liquid bottle II are located in the constant temperature chamber;

[0011] And an imaging and image acquisition unit is arranged above the sample stage unit, through which the images of multiple imprinting membranes to be detected arranged on the sample stage are simultaneously acquired.

[0012] The support frame comprises:

[0013] Base;

[0014] An upper mounting plate arranged parallel to the base plate;

[0015] and a support column connected between the base and the upper mounting plate.

[0016] The middle part of the sample stage is an observation glass, and the observation glass is a one-way perspective mirror.

[0017] The position adjustment structure comprises:

[0018] Two symmetrically arranged I-shaped fixing plates, the I-shaped fixing plates are fixed on the base of the support frame, and the two transverse plates of the I-shaped fixing plates are arranged along the X direction;

[0019] Four horizontal slide rails, the fixed ends of the four horizontal slide rails are arranged on the horizontal plates on the sides opposite to the I-shaped fixed plate;

[0020] Four lifting rails, two of which are connected between two horizontal plates of one I-shaped fixed plate, and the other two are connected between two horizontal plates of another I-shaped fixed plate; the fixed end of each lifting rail is fixedly connected to the sliding ends of two horizontal rails on the two horizontal plates of the corresponding I-shaped fixed plate; the side of the sample table is fixedly connected to the sliding end of the lifting rail;

[0021] And an electric push rod, through which the sample table moves up and down along the lifting slide rail.

[0022] The fluid delivery control structure comprises:

[0023] A liquid flow pipeline, one end of which is connected to the nozzle;

[0024] A peristaltic pump, wherein the other end of the liquid flow pipeline is connected to the output end of the peristaltic pump;

[0025] A solenoid valve group connected between the peristaltic pump and chemical color developing solution bottle I and chemical color developing solution bottle II, and a relay group connected to the peristaltic pump;

[0026] And a pipeline flushing bottle, the liquid inlet of the peristaltic pump is connected with the pipeline flushing bottle through the electromagnetic valve group.

[0027] The fluid delivery control structure also includes a waste liquid collection bottle arranged on the support frame, through which the color developing liquid mixture that is not sprayed after mixing is collected.

[0028] The nozzle motion control unit comprises:

[0029] A longitudinal motor group I and a longitudinal motor group II are symmetrically arranged on the base of the support frame along the X direction;

[0030] Two vertical frames arranged in parallel along the Y direction, the longitudinal motor group I is connected to the lower end of one of the vertical frames to drive it to move along the X direction, and the longitudinal motor group II is connected to the lower end of the other vertical frame to drive it to move along the X direction;

[0031] A horizontal beam connected to the other ends of the two vertical frames;

[0032] A transverse motor disposed at one end of the crossbeam;

[0033] And a mounting plate for mounting the nozzle, the mounting plate and the crossbeam are slidably matched along the length direction of the crossbeam, and the transverse motor drives the mounting plate to slide relative to the crossbeam through a power transmission structure.

[0034] The power transmission structure comprises:

[0035] A driving wheel coaxially fixedly connected to the output shaft of the transverse motor, wherein the axis direction of the driving wheel is along the Z direction;

[0036] a driven wheel disposed at the other end of the beam;

[0037] A transmission belt connected between the driving wheel and the driven wheel;

[0038] And a power transmission wheel is arranged on the transmission belt, and the power transmission wheel is fixedly connected to the mounting plate.

[0039] The constant temperature unit also includes:

[0040] A Peltier group, wherein a refrigeration section of the Peltier group is flush with the interior of the constant temperature chamber;

[0041] A heat sink, one end of which is bonded to the hot end of the Peltier group;

[0042] a cooling fan disposed at the other end of the radiator;

[0043] And a temperature sensor is arranged on the inner wall of the constant temperature chamber.

[0044] The imaging and image acquisition unit comprises:

[0045] An array light source is arranged on the lower end surface of the sample stage;

[0046] A cooling camera disposed on an upper mounting plate of the support frame;

[0047] A lens set on a cooled camera;

[0048] A filter wheel arranged perpendicular to the optical axis of the refrigerated camera, wherein a plurality of filters are evenly distributed on the circumference of the filter wheel;

[0049] A switching motor, wherein the switching motor drives the filter wheel to rotate through a belt transmission mechanism to switch any filter to be coaxial with the cooling camera;

[0050] And an industrial touch-control integrated machine, through which the switching motor and the refrigeration camera are controlled to collect images.

[0051] The beneficial effects of the present invention are as follows: a chemiluminescent imaging device for multi-region sprinkling of samples of the present invention obtains the image of the sample of the imprinted membrane to be detected through the imaging and image acquisition unit, and obtains the minimum rectangular position and area of ​​each imprinted membrane sample to be detected after image processing by the industrial touch-control integrated machine. The nozzle motion control unit and the color developing liquid spraying unit are controlled to spray the chemiluminescent color developing liquid uniformly on the surface of the imprinted membrane sample to be detected at a density of 100-200 μL / cm2. The specific process is: control the solenoid valve group, the relay group and the peristaltic pump to uniformly mix the chemiluminescent color developing liquid A and the chemiluminescent color developing liquid B in a ratio of 1:1 in the liquid flow pipeline to form a chemiluminescent color developing liquid. Control the longitudinal motor group I, the longitudinal motor group II and the transverse motor to move the nozzle to the area directly above the imprinted membrane sample to be detected, and complete the spraying of the chemiluminescent color developing liquid. After the spraying is completed, wait for 10 to 60 seconds, select a suitable filter, and use the imaging and image acquisition unit to obtain the chemiluminescent image of the imprinted membrane sample to be detected, so as to realize the chemiluminescent imaging of multi-region sprinkling. The present invention can effectively solve the problems of uneven sample rinsing and waste of color developing solution in chemiluminescent immunoblotting experiments, and can simultaneously process multiple blot membrane samples to be detected, which helps to improve detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 It is a schematic diagram of the overall structure of a multi-region chemiluminescent imaging device of the present invention;

[0053] Figure 2 It is a front view of a multi-region chemiluminescent imaging device of the present invention;

[0054] Figure 3 It is a rear view of a chemiluminescent imaging device for multi-region shower samples of the present invention;

[0055] Figure 4 It is a right view of a multi-region chemiluminescent imaging device of the present invention;

[0056] Figure 5 for Figure 2 AA section view;

[0057] Among them: 1. Support frame, 101. Base, 102. Upper mounting plate, 103. Pillar, 2. Sample stage unit, 201. Sample stage, 202. I-type fixing plate, 203. Horizontal slide rail, 204. Lifting slide rail, 205. Electric push rod, 3. Color liquid spray unit, 301. Nozzle, 302. Chemical color liquid bottle I, 303. Chemical color liquid bottle II, 304. Liquid flow pipeline, 305. Peristaltic pump, 306. Relay group, 307. Solenoid valve group, 308. Pipeline flushing bottle, 309. Waste liquid collection bottle, 4. Nozzle transport Motion control unit, 401, longitudinal motor group I, 402, longitudinal motor group II, 403, vertical frame, 404, beam, 405, transverse motor, 406, mounting plate, 5, constant temperature unit, 501, constant temperature room, 502, Peltier group, 503, radiator, 504, cooling fan, 6, imaging and image acquisition unit, 601, array light source, 602, cooling camera, 603, lens, 604, filter wheel, 605, switching motor, 606, guide plate, 607, industrial touch all-in-one machine, 7, imprinted membrane to be detected. DETAILED DESCRIPTION

[0058] The embodiments of the present invention will be further described below in conjunction with the accompanying drawings.

[0059] See also Figure 1-Figure 5 A multi-region chemiluminescent imaging device of the present invention comprises:

[0060] Support frame 1;

[0061] A sample stage unit 2 is arranged in the support frame 1, and the sample stage unit 2 at least includes a sample stage 201 and a position adjustment structure that drives the sample stage 201 to adjust relative to the support frame 1 along the X direction and the Z direction;

[0062] A color developing liquid spraying unit 3 is arranged above the sample stage 201, and the color developing liquid spraying unit 3 at least comprises a nozzle 301, a chemical color developing liquid bottle I 302, a chemical color developing liquid bottle II 303, and a fluid delivery control structure, and the different color developing liquids in the chemical color developing liquid bottle I 302 and the chemical color developing liquid bottle II 303 are delivered to the nozzle 301 and sprayed according to a set mixing ratio through the fluid delivery control structure;

[0063] A nozzle motion control unit 4, through which the nozzle motion control unit 4 adjusts and controls the movement of the nozzle 301 in the Y direction and the Z direction;

[0064] A constant temperature unit 5, wherein the constant temperature unit 5 at least comprises a constant temperature chamber 501, and a chemical color developing liquid bottle I 302 and a chemical color developing liquid bottle II 303 are located in the constant temperature chamber 501;

[0065] And an imaging and image acquisition unit 6 is arranged above the sample stage unit 2, and the imaging and image acquisition unit 6 simultaneously acquires images of multiple imprinting membranes 7 to be detected arranged on the sample stage 201.

[0066] The support frame 1 comprises:

[0067] Base 101;

[0068] An upper mounting plate 102 arranged parallel to the base plate 101;

[0069] And a support column 103 connected between the base 101 and the upper mounting plate 102 .

[0070] The middle part of the sample stage 201 is an observation glass, and the observation glass is a one-way perspective mirror.

[0071] The position adjustment structure comprises:

[0072] Two symmetrically arranged I-shaped fixing plates 202, wherein the I-shaped fixing plates 202 are fixed to the base 101 of the support frame 1, and two transverse plates of the I-shaped fixing plates 202 are arranged along the X direction;

[0073] Four horizontal slide rails 203, the fixed ends of the four horizontal slide rails 203 are arranged on the horizontal plates on the sides opposite to the I-shaped fixed plate 202;

[0074] Four lifting rails 204, two of which are connected between two horizontal plates of one I-shaped fixed plate 202, and the other two are connected between two horizontal plates of another I-shaped fixed plate 202; the fixed end of each lifting rail 204 is fixedly connected to the sliding ends of two horizontal rails 203 on the two horizontal plates of the corresponding I-shaped fixed plate 202; the side of the sample table 201 is fixedly connected to the sliding end of the lifting rail 204; the sliding end of the horizontal rail 203 is slidably matched with the fixed end of the horizontal rail 203, and the sliding end of the lifting rail 204 is slidably matched with the fixed end of the lifting rail 204;

[0075] And an electric push rod 205, through which the sample stage 201 moves up and down along the lifting slide rail 204.

[0076] In this embodiment, the electric push rod 205 pushes the sample stage 201 and the array light source 601 to move up and down along the lifting rail 204 as a whole, and the sample stage 201, the array light source 601 and the lifting rail 204 are pushed manually to move along the horizontal rail 203 as a whole.

[0077] The imprinted membrane 7 to be detected in the present invention refers to nitrocellulose membrane, nylon membrane, etc., with a thickness of about 0.1 mm. The imprinted membrane 7 to be detected is laid flat on the glass in the observation area in the middle of the sample stage 201. The observation glass is a one-way perspective mirror. The spacing between the samples of the imprinted membrane 7 to be detected should be greater than 1 mm.

[0078] The fluid delivery control structure comprises:

[0079] A liquid flow pipeline 304, one end of which is connected to the nozzle 301;

[0080] A peristaltic pump 305, wherein the other end of the liquid flow pipeline 304 is connected to the output end of the peristaltic pump 305;

[0081] A solenoid valve group 307 connected between the peristaltic pump 305 and the chemical color developing liquid bottle I 302 and the chemical color developing liquid bottle II 303, and a relay group 306 connected to the peristaltic pump 305; fluid delivery direction: chemical color developing liquid bottle I 302 and chemical color developing liquid bottle II 303-solenoid valve group 307-peristaltic pump 305; the relay group 306 controls the peristaltic pump alone;

[0082] The peristaltic pump 305 has a liquid inlet connected to the pipeline flushing bottle 308 through the electromagnetic valve group 307. The liquid entering the peristaltic pump through the electromagnetic valve group is the liquid in the chemical color developing liquid bottle I 302 and the chemical color developing liquid bottle II 303 or the liquid in the pipeline flushing bottle 308.

[0083] The fluid delivery control structure further includes a waste liquid collection bottle 309 disposed on the support frame 1, and the color developing liquid mixture that is not sprayed after mixing is collected through the waste liquid collection bottle 309. The waste liquid collection bottle 309 is in a horizontal straight line with the nozzle 301, and the nozzle 301 sprays the remaining waste liquid after spraying into the waste liquid collection bottle 309.

[0084] The chemical coloring liquid bottle I 302 of the fluid control unit and the chemiluminescent coloring liquid A and chemiluminescent coloring liquid B in the chemical coloring liquid bottle II 303 are uniformly mixed in a ratio of 1:1 to form a chemiluminescent coloring liquid. The mixing process is in the liquid flow pipeline 304, and the chemiluminescent coloring liquid is finally sprayed out from the nozzle 301. During the experiment, the chemiluminescent coloring liquid is sprayed from the nozzle 301 to the sample of the blot membrane 7 to be detected. After the experiment, the remaining liquid in the pipeline is sprayed from the nozzle 301 to the waste liquid collection bottle 309, and the peristaltic pump 305 is controlled by the cooperation of the solenoid valve group 307 and the relay group 306 to suck it into the waste liquid collection bottle 309 to complete the complete removal of the remaining liquid of the chemiluminescent coloring liquid.

[0085] The nozzle motion control unit 4 comprises:

[0086] A longitudinal motor group I 401 and a longitudinal motor group II 402 are symmetrically arranged on the base 101 of the support frame 1 along the X direction;

[0087] Two vertical frames 403 arranged in parallel along the Y direction, the longitudinal motor group I 401 is connected to the lower end of one of the vertical frames 403 to drive it to move along the X direction, and the longitudinal motor group II 402 is connected to the lower end of the other vertical frame 403 to drive it to move along the X direction;

[0088] A crossbeam 404 connected to the other ends of the two vertical frames 403;

[0089] A transverse motor 405 disposed at one end of the crossbeam 404;

[0090] And a mounting plate 406 for mounting the nozzle 301, the mounting plate 406 and the cross beam 404 are slidably matched along the length direction of the cross beam 404, and the transverse motor 405 drives the mounting plate 406 to slide relative to the cross beam 404 through a power transmission structure.

[0091] In this embodiment, the longitudinal motor group I 401 and the longitudinal motor group II 402 have the same structure, and the specific structure includes a driving motor, a screw coaxially fixedly connected to the output shaft of the driving motor, a guide rod arranged parallel to the screw, and a nut forming a screw nut pair with the screw, and the lower end of the vertical frame 403 is fixedly connected to the nut.

[0092] The power transmission structure comprises:

[0093] A driving wheel coaxially fixedly connected to the output shaft of the transverse motor 405, wherein the axis direction of the driving wheel is along the Z direction;

[0094] A driven wheel disposed at the other end of the crossbeam 404;

[0095] A transmission belt connected between the driving wheel and the driven wheel;

[0096] And a power transmission wheel is arranged on the transmission belt, and the power transmission wheel is fixedly connected to the mounting plate 406.

[0097] The longitudinal motor group I 401 and the longitudinal motor group II 402 in the nozzle motion control unit 4 are located on both sides of the sample stage 201 and are installed on both sides of the base 101 by screws. A vertical frame 403 is installed on the sliders of the longitudinal motor group I 401 and the longitudinal motor group II 402 respectively, and the crossbeam 404 is fixed on the two vertical frames 403 by screws, that is, the longitudinal motor group I 401 and the longitudinal motor group II 402 are connected through the vertical frame 403 and the crossbeam 404, when the longitudinal motor group I 401 and the longitudinal motor group II 402 are synchronously controlled. The mounting plate 406 of the nozzle 301 can slide on the slideway on the crossbeam 404. The transverse motor 405 is fixed on the vertical frame 403 corresponding to the longitudinal motor group I 401, and the transverse motor 405 drives the nozzle 301 mounting plate 406 to make linear motion on the slideway on the crossbeam 404 through a pulley.

[0098] The constant temperature unit 5 also includes:

[0099] A Peltier group 502, wherein a cooling section of the Peltier group 502 is flush with the interior of the constant temperature chamber 501;

[0100] A heat sink 503, one end of which is attached to the hot end of the Peltier group 502;

[0101] A cooling fan 504 is provided at the other end of the radiator 503;

[0102] And a temperature sensor is arranged on the inner wall of the constant temperature chamber 501.

[0103] In this embodiment, the constant temperature chamber 501 is a 5° C. constant temperature chamber 501 .

[0104] The chemical coloring liquid bottle I 302 and the chemical coloring liquid bottle II 303 are placed in a 5°C constant temperature room 501 for long-term storage. The top of the 5°C constant temperature room 501 has an opening of a size similar to that of the liquid flow pipe 304, and the liquid flow pipe 304 hose is connected to the chemical coloring liquid bottle I 302 and the chemical coloring liquid bottle II 303. The cooling end of the Peltier group 502 is flush with the inner wall of the 5°C constant temperature control room, and the radiator 503 is attached to the hot end of the Peltier group 502. The other end of the radiator 503 is equipped with a cooling fan 504. The temperature sensor is installed on the inner wall of the 5°C constant temperature room 501.

[0105] The imaging and image acquisition unit 6 comprises:

[0106] An array light source 601 disposed on the lower end surface of the sample stage 201;

[0107] A cooling camera 602 disposed on the upper mounting plate 102 of the support frame 1;

[0108] A lens 603 disposed on the cooling camera 602;

[0109] A filter wheel 604 is arranged perpendicular to the optical axis direction of the cooling camera 602, and a plurality of filters are evenly distributed on the circumference of the filter wheel 604;

[0110] A switching motor 605, wherein the switching motor 605 drives the filter wheel 604 to rotate through a belt transmission mechanism to switch any filter to be coaxial with the cooling camera 602;

[0111] And an industrial touch-control integrated machine 607, through which the switching motor 605 and the cooling camera 602 are controlled to collect images.

[0112] In this embodiment, the imaging and image acquisition unit 6 also includes a guide plate 606 fixedly connected to the upper mounting plate 102 of the support frame 1, and a light hole is provided on the guide plate 606, and the light hole and the refrigerated camera 602 are coaxially arranged; the switching motor 605 drives the filter wheel 604 to rotate through the belt transmission mechanism to switch any one of the filters and the refrigerated camera 602 coaxially. Specifically, the filter wheel 604 rotates coaxially with respect to the upper mounting plate 102 of the support frame 1, and the filter wheel 604 shaft serves as a large pulley, and a small pulley is provided on the switching motor 605. The large pulley and the small pulley are connected by a belt, and then the filter wheel 604 is driven by the switching motor 605 to rotate to realize the switching of the filter.

[0113] The array light source 601 is located directly below the sample stage 201 and provides a backlight source for imaging observation. The array light source 601 and the sample stage 201 are installed as a whole through threads. The lens 603 is installed on the cooling camera 602 through threads. The centers of each filter of the filter wheel 604 coincide with the optical axis of the lens 603. The filter wheel 604 switching motor 605 drives the filter wheel 604 through a pulley to complete the switching of the filter. The electric push rod 205 is located below the center of the array light source 601. There is a certain gap between the upper surface of the electric push rod 205 connector and the lower surface of the array light source 601. As the connector approaches the direction of the lens 603 until the entire array light source 601 and the sample stage 201 are pushed to move linearly in the direction of the lens 603, the above gap disappears. The vertical surface of the lifting rail 204 is perpendicular to the upper plane of the sample stage 201. The sliding end of the lifting rail 204 is fixed to the side of the sample stage 201, and the fixed ends of the four lifting rails 204 are respectively fixed to the sliding ends of the four horizontal rails 203. The fixed end of the horizontal rail 203 is fixed to the I-shaped fixing plate 202. The I-shaped fixing plate 202 is fixed to the base 101 by screws. The electric push rod 205 provides linear motion for the array light source 601 and the sample stage unit 2, and the motion direction is along the vertical direction of the lifting rail 204, which is used to control the distance between the upper surface of the sample stage 201 and the lowermost surface of the lens 603 to find the best focusing position. The sample stage 201 can make linear motion along the horizontal rail 203 when pushed by the experimenter, so that the experimenter can push the sample stage 201 to a position convenient for operation.

[0114] The working process of a multi-region chemiluminescent imaging device for sprinkling samples of the present invention is as follows: a single or multiple imprinted membranes 7 to be detected are laid flat on the upper surface observation area of ​​the sample stage 201, and the intervals between each imprinted membrane 7 to be detected are greater than 1 mm. The array light source 601 and the refrigerated camera 602 are controlled to collect images with light source but without chemiluminescent color-developing liquid in a dark room, and the acquired images are morphologically processed by the industrial touch-control integrated machine 607 to analyze the area and position of each imprinted membrane. The color-developing liquid spraying unit 3 is controlled to obtain a chemiluminescent color-developing liquid prepared by mixing chemiluminescent color-developing liquid A and chemiluminescent color-developing liquid B in a ratio of 1:1, and the liquid is driven to the nozzle 301. The nozzle motion control unit 4 is controlled to open the nozzle 301 when the nozzle 301 is above the imprinted membrane, and uniform sprinkling of multiple imprinted membranes 7 to be detected is completed, and the sprinkling density is 100-200μL / cm 2After the sample is sprayed, wait for 0-60 seconds, control the refrigerated camera 602 to collect images without light source and with chemiluminescent color developing solution in the dark room, and process and store the images. Within 24 hours after the end of the experiment, control the color developing solution spray unit 3 to spray all the remaining chemiluminescent color developing solution in the liquid flow pipeline 304 into the waste liquid collection bottle 309, and absorb the pipeline flushing liquid into the liquid flow pipeline 304, and then spray the pipeline flushing liquid into the waste liquid collection bottle 309 to complete the pipeline cleaning.

Claims

1. A multi-region chemiluminescent imaging device, characterized in that: include: Support frame (1); A sample stage unit (2) arranged in a support frame (1), the sample stage unit (2) comprising at least a sample stage (201) and a position adjustment structure for driving the sample stage (201) to adjust relative to the support frame (1) along the X direction and the Z direction; A color developing liquid spraying unit (3) is arranged above the sample stage (201), the color developing liquid spraying unit (3) comprising at least a nozzle (301), a chemical color developing liquid bottle I (302), a chemical color developing liquid bottle II (303) and a fluid delivery control structure, and the different color developing liquids in the chemical color developing liquid bottle I (302) and the chemical color developing liquid bottle II (303) are delivered to the nozzle (301) according to a set mixing ratio through the fluid delivery control structure and sprayed; A nozzle motion control unit (4), which is used to adjust and control the movement of the nozzle (301) in the Y direction and the Z direction; A constant temperature unit (5), the constant temperature unit (5) comprising at least a constant temperature chamber (501), wherein a chemical color developing liquid bottle I (302) and a chemical color developing liquid bottle II (303) are located in the constant temperature chamber (501); and an imaging and image acquisition unit (6) arranged above the sample stage unit (2), through which images of a plurality of imprinted membranes (7) to be detected arranged on the sample stage (201) are simultaneously acquired.

2. A multi-region chemiluminescent imaging device according to claim 1, characterized in that: The support frame (1) comprises: Base (101); An upper mounting plate (102) arranged parallel to the base plate (101); and a support column (103) supporting and connecting between the base (101) and the upper mounting plate (102).

3. A multi-region chemiluminescent imaging device for shower samples according to claim 1 or 2, characterized in that: The middle part of the sample stage (201) is an observation glass, and the observation glass is a one-way perspective mirror.

4. The multi-region chemiluminescent imaging device of claim 1, characterized in that: The position adjustment structure comprises: Two symmetrically arranged I-shaped fixing plates (202), wherein the I-shaped fixing plates (202) are fixed on the base (101) of the support frame (1), and two transverse plates of the I-shaped fixing plates (202) are arranged along the X direction; Four horizontal slide rails (203), wherein the fixed ends of the four horizontal slide rails (203) are arranged on the horizontal plates on the sides opposite to the I-shaped fixed plate (202); Four lifting rails (204), two of which are connected between two transverse plates of one I-shaped fixed plate (202), and the other two of which are connected between two transverse plates of another I-shaped fixed plate (202); the fixed end of each lifting rail (204) is fixedly connected to the sliding ends of two horizontal rails (203) on the two transverse plates of the corresponding I-shaped fixed plate (202); the side of the sample table (201) is fixedly connected to the sliding end of the lifting rail (204); and an electric push rod (205), through which the sample platform (201) moves up and down along the lifting slide rail (204).

5. The multi-region chemiluminescent imaging device of claim 1, characterized in that: The fluid delivery control structure comprises: A liquid flow pipeline (304), one end of which is connected to the nozzle (301); A peristaltic pump (305), wherein the other end of the liquid flow pipeline (304) is connected to an output end of the peristaltic pump (305); A solenoid valve group (307) connected between the peristaltic pump (305) and the chemical color developing solution bottle I (302) and the chemical color developing solution bottle II (303), and a relay group (306) connected to the peristaltic pump (305); and a pipeline flushing bottle (308); the liquid inlet of the peristaltic pump (305) is connected to the pipeline flushing bottle (308) through a solenoid valve group (307).

6. The multi-region chemiluminescent imaging device of claim 5, characterized in that: The fluid delivery control structure also includes a waste liquid collection bottle (309) arranged on the support frame (1), and the color developing liquid mixture that is not sprayed after mixing is collected through the waste liquid collection bottle (309).

7. The multi-region chemiluminescent imaging device of claim 1, characterized in that: The nozzle motion control unit (4) comprises: A longitudinal motor group I (401) and a longitudinal motor group II (402) are symmetrically arranged on the base (101) of the support frame (1) along the X direction; Two vertical frames (403) arranged in parallel along the Y direction, the longitudinal motor group I (401) is connected to the lower end of one of the vertical frames (403) to drive it to move along the X direction, and the longitudinal motor group II (402) is connected to the lower end of the other vertical frame (403) to drive it to move along the X direction; A cross beam (404) connected to the other ends of the two vertical frames (403); A transverse motor (405) disposed at one end of the crossbeam (404); and a mounting plate (406) for mounting the nozzle (301); the mounting plate (406) and the crossbeam (404) are slidably matched along the length direction of the crossbeam (404); and the transverse motor (405) drives the mounting plate (406) to slide relative to the crossbeam (404) through a power transmission structure.

8. The multi-region chemiluminescent imaging device of claim 7, characterized in that: The power transmission structure comprises: A driving wheel coaxially fixedly connected to the output shaft of the transverse motor (405), wherein the axis direction of the driving wheel is along the Z direction; A driven wheel disposed at the other end of the crossbeam (404); A transmission belt connected between the driving wheel and the driven wheel; And a power transmission wheel is arranged on the transmission belt, and the power transmission wheel is fixedly connected to the mounting plate (406).

9. The multi-region chemiluminescent imaging device of claim 1, characterized in that: The constant temperature unit (5) further comprises: A Peltier group (502), wherein a cooling section of the Peltier group (502) is flush with the interior of the constant temperature chamber (501); A heat sink (503), one end of the heat sink (503) being bonded to the hot end of the Peltier group (502); A cooling fan (504) disposed at the other end of the radiator (503); And a temperature sensor is arranged on the inner wall of the constant temperature chamber (501).

10. The multi-region chemiluminescent imaging device of claim 1, characterized in that: The imaging and image acquisition unit (6) comprises: An array light source (601) disposed on the lower end surface of the sample stage (201); a cooling camera (602) arranged on an upper mounting plate (102) of the support frame (1); A lens (603) disposed on the cooling camera (602); A filter wheel (604) arranged perpendicular to the optical axis direction of the cooling camera (602), wherein a plurality of filters are evenly distributed on the circumference of the filter wheel (604); A switching motor (605), wherein the switching motor (605) drives the filter wheel (604) to rotate through a belt transmission mechanism to switch any filter to be coaxial with the cooling camera (602); and an industrial touch-control integrated machine (607), which controls a switching motor (605) and a cooling camera (602) to collect images.

Images