A kind of disinfection method for preventing silkworm pus disease

Abstract

The invention discloses a disinfection method for preventing silkworm pus disease, which comprises disinfecting silkworm houses and silkworm bodies regularly; the method for disinfecting silkworm houses comprises: spraying silkworm house floors, walls, and tools and equipment with alkaline aqueous solution, and spraying silkworm house floors, walls, and tools and equipment with silkworm house disinfectant after air drying; sealing the silkworm house for 30 to 60 minutes after spraying the silkworm house disinfectant; the silkworm house disinfectant comprises peracetic acid with a mass concentration of 0.01 to 0.2%, povidone iodine with a mass concentration of 2 to 5%, and lemon essential oil with a mass concentration of 2 to 5%, and the rest is water; the method for disinfecting silkworm bodies comprises: spraying a layer of mist on silkworm bodies with alkaline aqueous solution; and spraying silkworm bodies with silkworm body disinfectant after the alkaline aqueous solution is air dried. The disinfection method of the invention can significantly improve the disinfection effect and greatly reduce the incidence of silkworm pus disease.

Description

Technical Field

[0001] The invention relates to the field of silkworm breeding, and in particular to a disinfection method for preventing silkworm pus disease. Background Art

[0002] Silkworms are prone to silkworm pus disease during the breeding process, and it occurs in May, June and July of the silkworm season in spring, and September and October of the silkworm season in autumn. Silkworm pus disease is caused by polyhedrosis virus infection. At present, the prevention of polyhedrosis virus generally adopts alkaline solutions such as lime water in combination with silkworm disinfectants for disinfection. However, when alkaline solutions are mixed with disinfectants, on the one hand, some disinfectants cannot be mixed with alkaline solutions, and the selection of disinfectants is extremely demanding. On the other hand, the disinfectant needs to wait for the alkaline solution to melt the virus shell before it can play its own role and kill the virus completely. During this period, the disinfectant may be oxidized due to long-term exposure to the air, resulting in reduced disinfection efficacy. Therefore, it is necessary to increase the amount of disinfectant used to prevent silkworm pus disease. If the amount of disinfectant sprayed is too large, the humidity in the silkworm body and the environment will be too high, which is not conducive to the growth of silkworms.

[0003] It is known in the prior art that peracetic acid has a killing effect on bacteria, spores, fungi and viruses, and has a spectrum of bactericidal properties. The use of peracetic acid to disinfect silkworm bodies can replace the disinfectant composed of multiple disinfectants and bactericides in the prior art. However, peracetic acid is unstable and is not suitable for mixing with a strong alkaline solution. In addition, a high concentration of peracetic acid has a strong pungent odor. Too high a concentration has a strong irritating effect on the eyes, skin, mucous membranes and upper respiratory tract of the human body. Therefore, it is only suitable for use at a relatively low concentration. Therefore, it is necessary to reasonably utilize peracetic acid and alkaline solutions, reduce the amount of drugs used, and improve the prevention and control effect. Summary of the invention

[0004] The invention overcomes the technical problems in the prior art that disinfection using a mixture of an alkaline solution and other disinfectants results in reduced disinfection efficacy and that peracetic acid cannot well exert its disinfection and sterilization effects, and provides a disinfection method for preventing silkworm pus disease.

[0005] A disinfection method for preventing silkworm pus disease, the method comprising regularly disinfecting silkworm rooms and silkworm bodies;

[0006] The method for disinfecting a silkworm house comprises: spraying the floor, walls, and equipment of the silkworm house with an alkaline aqueous solution, and then spraying the floor, walls, and equipment of the silkworm house with a silkworm house disinfectant after air drying; sealing the silkworm house for 30 to 60 minutes after spraying the silkworm house disinfectant; the silkworm house disinfectant contains peracetic acid with a mass concentration of 0.01 to 0.2%, povidone iodine with a mass concentration of 2 to 5%, and lemon essential oil with a mass concentration of 2 to 5%, and the rest is water;

[0007] The method for disinfecting silkworm bodies comprises: spraying a layer of mist on the silkworm bodies with an alkaline aqueous solution; spraying the silkworm bodies with a silkworm body disinfectant after the alkaline aqueous solution is air-dried; the silkworm body disinfectant contains peracetic acid with a mass concentration of 0.5-0.8%, povidone iodine with a mass concentration of 5-8%, lemon essential oil with a mass concentration of 2-5%, and the rest is water.

[0008] Furthermore, the alkaline aqueous solution is a lime water solution; the lime water solution is prepared by the following method: soaking lime blocks in water, and the ratio of lime water to water is 1kg:10L.

[0009] Furthermore, the spraying amount of the silkworm room disinfectant is to spray a layer of water mist on the surface of the silkworm room floor, walls, and utensils and equipment.

[0010] Furthermore, the spraying amount of the silkworm body disinfectant is to spray a layer of water mist on the surface of the silkworm body.

[0011] Furthermore, the silkworm room disinfectant and silkworm body disinfectant are both prepared and used immediately.

[0012] Furthermore, the silkworm house is disinfected once every month; and the silkworm bodies are disinfected once for each age group.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] (1) In the process of disinfecting the silkworm house and the silkworm body, the present invention first sprays the silkworm house and the silkworm body with an alkaline aqueous solution, and then sprays the disinfectant after natural air drying. Compared with mixing the alkaline solution with the disinfectant, the mutual influence between the alkaline solution and the peracetic acid can be avoided, and the efficacy of the disinfectant will not be affected. Therefore, the present application chooses to use the disinfectant and the alkaline solution separately, and the alkaline aqueous solution is used first to dissolve the shell of the silkworm pus disease, and then the disinfectant is used to completely kill the bacteria. The use method of the present invention has a good sterilization effect.

[0015] (2) The silkworm house disinfectant and silkworm body disinfectant of the present invention are both composed of povidone iodine, lemon essential oil and peracetic acid. The present invention mixes peracetic acid with povidone iodine and lemon essential oil. On the one hand, the lemon essential oil is mixed with the peracetic acid solution, and the essential oil can float on the surface of the peracetic acid solution, which can volatilize the peracetic acid, thereby improving the stability of the peracetic acid. On the other hand, the combination of povidone iodine and peracetic acid can reduce the usage of peracetic acid and povidone iodine, and reduce the irritation of peracetic acid to the human body. Therefore, the disinfection method of the present invention can significantly improve the disinfection effect and greatly reduce the morbidity of silkworm pus disease. Specifically, peracetic acid has a strong oxidizing effect, which can oxidize bacterial proteins and kill microorganisms. It has an efficient and rapid bactericidal effect on a variety of microorganisms, including spores and viruses. Although it has a good bactericidal effect, it cannot maintain a long antibacterial effect. Although povidone iodine is also a common bactericide in this field, it is a broad-spectrum strong bactericidal disinfectant, which has a strong killing effect on viruses, bacteria, fungi and mold spores. It has little irritation to the skin, low toxicity, and long-lasting effect. However, povidone iodine can only have a good bactericidal effect when used at a dosage of about 10%. The concentration is high and the dosage is large. When used, in order to ensure the bactericidal effect, The use concentration is high and the dosage is large. When sprayed on the silkworm body, the humidity of the silkworm body is high, which easily causes the silkworm body to die due to excessive humidity. However, the povidone-iodine aqueous solution is acidic, and when used in combination with peracetic acid, the dosage of povidone-iodine can be greatly reduced. Peracetic acid has the characteristic of being volatile. Essential oil can improve the pungent smell of peracetic acid to a certain extent. At the same time, it is found through research that when lemon essential oil and peracetic acid are mixed according to the ratio of the present invention, the lemon essential oil can play a warning role. When the lemon essential oil is almost volatilized and the smell is less, the peracetic acid is almost volatile. Therefore, the lemon essential oil can be used to identify whether peracetic acid is still contained.

[0016] (3) The disinfectant formula of the present invention utilizes the high oxidative bactericidal effect of peracetic acid, and it has strong acidity. The feed and mulberry leaves eaten by silkworms are both acidic. According to the eating characteristics of silkworms, the present invention adjusts the ratio of peracetic acid and povidone iodine in the silkworm disinfectant to increase the acidity of the disinfectant, so that when a layer of water mist disinfectant is sprayed, peracetic acid can neutralize the alkaline aqueous solution, and a suitable growth environment and dietary environment can be provided for silkworms without washing the silkworm house and silkworm seat. The components in the disinfectant of the present invention complement each other and have a synergistic effect. The silkworm body and silkworm house can be disinfected safely at a low dose, with good bactericidal and disinfection effects and a low incidence of silkworm pus disease. DETAILED DESCRIPTION

[0017] The present invention will be further described below in conjunction with examples and tests.

[0018] Example 1

[0019] A disinfection method for preventing silkworm pus disease, the method comprising: regularly disinfecting silkworm houses and silkworm bodies; disinfecting silkworm houses once a month, and each time the silkworm houses are disinfected, the silkworm houses are disinfected after the frames are transferred; the method for disinfecting silkworm houses comprises: spraying the silkworm house floor, wall, and tools and equipment with an alkaline aqueous solution, and spraying the silkworm house floor, wall, and tools and equipment with a silkworm house disinfectant after air drying; during disinfection, spraying a layer of water mist on the surface of the silkworm house floor, wall, and tools and equipment; sealing the silkworm house for 60 minutes after spraying the silkworm house disinfectant, and not washing the silkworm house after disinfection; the silkworm house disinfectant contains 0.2% peroxyacetic acid by mass, 2% povidone iodine by mass, and 5% lemon essential oil by mass, and the rest is water;

[0020] The disinfection frequency of silkworm bodies is once for each age group, and the method of disinfecting silkworm bodies is as follows: spraying a layer of mist on the silkworm bodies with an alkaline aqueous solution; spraying the silkworm bodies with a silkworm body disinfectant after the alkaline aqueous solution is air-dried; during disinfection, spraying a layer of water mist on the surface of the silkworm bodies. The silkworm body disinfectant of this embodiment contains peracetic acid with a mass concentration of 0.8%, povidone iodine with a mass concentration of 5%, and lemon essential oil with a mass concentration of 5%, and the rest is water.

[0021] The alkaline aqueous solution is a lime water solution; the lime water solution is prepared by the following method: soaking lime blocks in water, and the ratio of lime water to water is 1kg:10L.

[0022] Example 2

[0023] A disinfection method for preventing silkworm pus disease, the method comprising: regularly disinfecting silkworm houses and silkworm bodies; disinfecting silkworm houses once a month, and each time the silkworm houses are disinfected, the silkworm houses are disinfected after the frames are transferred; the method for disinfecting silkworm houses comprises: spraying the silkworm house floor, walls, and utensils and equipment with an alkaline aqueous solution, and spraying the silkworm house floor, walls, and utensils and equipment with a silkworm house disinfectant after air drying; during disinfection, spraying a layer of water mist on the surface of the silkworm house floor, walls, and utensils and equipment; sealing the silkworm house for 30 minutes after spraying the silkworm house disinfectant, and not washing the silkworm house after disinfection; the silkworm house disinfectant of the present embodiment contains peracetic acid with a mass concentration of 0.01%, povidone iodine with a mass concentration of 5%, and lemon essential oil with a mass concentration of 2%, and the rest is water;

[0024] The disinfection frequency of silkworm bodies is once for each age group. The disinfection method of silkworm bodies is as follows: spray a layer of mist on the silkworm bodies with an alkaline aqueous solution; spray the silkworm bodies with a silkworm body disinfectant after the alkaline aqueous solution is air-dried; during disinfection, spray a layer of water mist on the surface of the silkworm bodies. The silkworm body disinfectant of this embodiment contains 0.5% peracetic acid, 8% povidone iodine and 2% lemon essential oil, and the rest is water.

[0025] The alkaline aqueous solution is a lime water solution; the lime water solution is prepared by the following method: soaking lime blocks in water, and the ratio of lime water to water is 1kg:10L.

[0026] Example 3

[0027] A disinfection method for preventing silkworm pus disease, the method comprising: regularly disinfecting silkworm houses and silkworm bodies; disinfecting silkworm houses once a month, and each time the silkworm houses are disinfected, the silkworm houses are disinfected after the frames are transferred; the method for disinfecting silkworm houses comprises: spraying the silkworm house floor, walls, and utensils and equipment with an alkaline aqueous solution, and spraying the silkworm house floor, walls, and utensils and equipment with a silkworm house disinfectant after air drying; during disinfection, spraying a layer of water mist on the surface of the silkworm house floor, walls, and utensils and equipment; sealing the silkworm house for 50 minutes after spraying the silkworm house disinfectant, and not washing the silkworm house after disinfection; the silkworm house disinfectant of the present embodiment contains peracetic acid with a mass concentration of 0.1%, povidone iodine with a mass concentration of 4%, and lemon essential oil with a mass concentration of 4%, and the rest is water;

[0028] The disinfection frequency of silkworm bodies is once for each age group, and the method of disinfecting silkworm bodies is as follows: spraying a layer of mist on the silkworm bodies with an alkaline aqueous solution; spraying the silkworm bodies with a silkworm body disinfectant after the alkaline aqueous solution is air-dried; during disinfection, spraying a layer of water mist on the surface of the silkworm bodies. The silkworm body disinfectant of this embodiment contains peracetic acid with a mass concentration of 0.7%, povidone iodine with a mass concentration of 7%, and lemon essential oil with a mass concentration of 4%, and the rest is water.

[0029] The alkaline aqueous solution is a lime water solution; the lime water solution is prepared by the following method: soaking lime blocks in water, and the ratio of lime water to water is 1kg:10L.

[0030] Experiment 1:

[0031] In order to illustrate the difference in technical effects between the separate use of the alkaline disinfectant and the disinfectant of the present invention and the mixed use of the alkaline solution and the commercially available agent in the prior art, the applicant conducted a silkworm breeding experiment and designed the following experimental groups:

[0032] Experimental group 1: disinfection was carried out using the disinfection method of Example 1 above.

[0033] Experimental group 2: disinfection was carried out using the disinfection method of Example 2 above.

[0034] Experimental group 3: disinfected by the disinfection method of Example 3 above.

[0035] Experimental Group 4: The disinfection was carried out using a disinfection method substantially the same as that in Example 1, except that the silkworm room disinfectant and silkworm body disinfectant in Experimental Group 4 were not disinfected with an alkaline aqueous solution before use.

[0036] Experimental Group 5: The disinfection method of Experimental Group 5 is to first use alkaline aqueous solution to disinfect the silkworm body and silkworm seat, and then use bleaching powder with an effective chlorine content of 0.3% to disinfect the silkworm body and silkworm seat.

[0037] Test method:

[0038] Pathogen preparation: Nuclear polyhedrosis virus suspension (1X10 8 CFU), cytoplasmic polyhedrosis virus suspension (1X10 8 CFU), Bacillus thuringiensis suspension (1X10 8 CFU), Serratia suspension (1X10 8 CFU), aflatoxin suspension (1X10 8 CFU)

[0039] Experimental treatment: spray the suspension on the bodies and silkworm seats of 3-year-old silkworms, and spray a layer of water mist; after the water mist on the silkworm bodies and silkworm seats is completely dry (about one hour), the silkworm bodies and silkworm rooms are disinfected by the disinfection methods of experimental groups 1 to 8. After 2 days of disinfection, the mortality rate of the silkworm bodies (mortality rate = number of deaths / total number of silkworms * 100%) and the incidence rate of silkworm pus disease (incidence rate of silkworm pus disease = number of infected silkworms / total number of silkworms * 100%) are observed. The results are shown in Table 1 below:

[0040] Table 1

[0041] Experimental Group Experimental Group 1 Experimental Group 2 Experimental Group 3 Experimental Group 4 Experimental Group 5 mortality rate(%) 2.6％ 3.4％ 5.6％ 82.4％ 34.1％ Incidence of silkworm pus disease (%) 0 0 0 93.6％ 46.3％

[0042] From the comparison of the effects of experimental groups 1 to 3 and experimental group 8 in Table 1, it can be seen that when the alkaline aqueous solution is not used for disinfection, the disinfection effect of the disinfectant is greatly reduced. From the comparison of the effects of experimental groups 1 to 3 and experimental group 9, it can be seen that the mixed use of peracetic acid, lemon essential oil and povidone iodine in the present invention is better than bleaching powder.

[0043] Experiment 2:

[0044] In order to study the bactericidal effect of peracetic acid, povidone iodine and lemon essential oil, different concentration treatment groups were designed: the treatment groups are as follows:

[0045] Treatment group 1: 1% peracetic acid aqueous solution, freshly prepared;

[0046] Treatment group 2: 2% peracetic acid aqueous solution, freshly prepared;

[0047] Treatment group 3: 5% povidone-iodine aqueous solution, freshly prepared;

[0048] Treatment group 4: 10% povidone-iodine aqueous solution, freshly prepared;

[0049] Treatment group 5: 5% lemon essential oil in water, freshly prepared, shaken well before use;

[0050] Treatment group 6: 0.5% peracetic acid and 2% povidone-iodine mixed aqueous solution, freshly prepared;

[0051] Treatment group 7: 5% lemon essential oil and 2% povidone-iodine mixed aqueous solution, freshly prepared;

[0052] Treatment group 8: 5% lemon essential oil and 0.5% peracetic acid mixed in water, freshly prepared;

[0053] Pathogen preparation: Nuclear polyhedrosis virus suspension (1X10 8 CFU), cytoplasmic polyhedrosis virus suspension (1X10 8 CFU), Bacillus thuringiensis suspension (1X10 8 CFU), Serratia suspension (1X10 8 CFU), aflatoxin suspension (1X10 8 CFU)

[0054] Experimental treatment: The suspension was sprayed on the bodies and silkworm seats of 3-year-old silkworms, and a layer of water mist was sprayed; after the water mist on the silkworm bodies and silkworm seats was completely dried (about one hour), they were first disinfected with the alkaline aqueous solution of Example 1, and after air-drying, they were immediately disinfected with the disinfectants of treatment groups 1-7; one day after disinfection, the incidence of silkworm pus disease (silkworm pus disease incidence rate = number of silkworms with disease / total number of silkworms * 100%) and other silkworm diseases (other silkworm disease incidence rate = number of silkworms with other silkworm diseases except silkworm pus disease / total number of silkworms * 100%) was observed, and the results were as shown in Table 2:

[0055] Table 2

[0056]

[0057]

[0058] It can be seen from Table 2 that the higher the concentrations of peracetic acid and povidone iodine, the better the pathogen control effect. It can be seen from treatment group 5 that the use of lemon essential oil alone has no effect on the control of silkworm pathogens. By comparing treatment groups 6 to 8 with treatment groups 2 and 4, it can be seen that the use of peracetic acid and povidone iodine can reduce the amount of drugs used. The combined use of lemon essential oil and other drugs can also improve the control effect of silkworm pus disease to a certain extent.

[0059] The above experiments 1 and 2 show that the combined use of peracetic acid, povidone iodine and lemon essential oil can greatly improve the prevention and treatment effects of silkworm pus disease. The combination of the three can reduce the concentration of each drug. Peracetic acid, povidone iodine and lemon essential oil have a synergistic effect with significant effects!

[0060] The above description is a detailed description of the preferred feasible embodiments of the present invention, but the embodiments are not intended to limit the scope of the patent application of the present invention. All equivalent changes or modified changes completed under the technical spirit suggested by the present invention should fall within the patent scope covered by the present invention.

Claims

1. A disinfection method for preventing silkworm pus disease, characterized in that: The method comprises the following steps: regularly disinfecting the silkworm house and the silkworm bodies; the method for disinfecting the silkworm house comprises: spraying the silkworm house floor, walls, and utensils and equipment with an alkaline aqueous solution, and spraying the silkworm house floor, walls, and utensils and equipment with a silkworm house disinfectant after air drying; sealing the silkworm house for 30 to 60 minutes after spraying the silkworm house disinfectant; the silkworm house disinfectant comprises peracetic acid with a mass concentration of 0.01 to 0.2%, povidone iodine with a mass concentration of 2 to 5%, and lemon essential oil with a mass concentration of 2 to 5%, and the rest is water; The method for disinfecting silkworm bodies comprises: spraying a layer of mist on the silkworm bodies with an alkaline aqueous solution; spraying the silkworm bodies with a silkworm body disinfectant after the alkaline aqueous solution is air-dried; the silkworm body disinfectant contains peracetic acid with a mass concentration of 0.5-0.8%, povidone iodine with a mass concentration of 5-8%, and lemon essential oil with a mass concentration of 2-5%, and the rest is water; The alkaline aqueous solution is a lime water solution; the lime water solution is prepared by the following method: soaking lime blocks in water, and the ratio of lime water to water is 1kg:10L.

2. A disinfection method for preventing silkworm pus disease as claimed in claim 1, characterized in that: The spraying amount of the silkworm room disinfectant is to spray a layer of water mist on the surface of the silkworm room floor, walls, and utensils and equipment.

3. A disinfection method for preventing silkworm pus disease as claimed in claim 1, characterized in that: The spraying amount of the silkworm body disinfectant is to spray a layer of water mist on the surface of the silkworm body.

4. A disinfection method for preventing silkworm pus disease as claimed in claim 1, characterized in that: The silkworm room disinfectant and silkworm body disinfectant are both prepared and used immediately.

5. A disinfection method for preventing silkworm pus disease as claimed in claim 1, characterized in that: The silkworm room is disinfected once every month; the silkworm bodies are disinfected once for each age group.

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