Strains and their applications

Abstract

The present invention provides a strain and its application, belonging to the field of microbial technology. The growth of the strain is basically unaffected when exposed to cadmium, and it can not only cope with high concentrations of cadmium stress (MIC is 350 mg / L); but also has good tolerance to heavy metals copper and zinc, with MICs of 800 mg / L and 550 mg / L, respectively. In addition, the strain can also significantly change the pH of the culture medium. The clearance experiment shows that the strain can remove most of the cadmium in LB culture medium and basically remove all of the cadmium in 0.85% NaCl, and is suitable for the restoration of contaminated water bodies, effectively solving the technical problems that the microbial strains currently used to repair cadmium-containing soil or water bodies have poor tolerance to heavy metals such as copper, zinc, and cadmium and poor restoration effect.

Description

Technical Field

[0001] The present application relates to the field of microbial technology, and in particular to a strain and its application. Background Art

[0002] With the rapid development of modern industrialization, soil and water bodies are polluted by heavy metals, among which cadmium is one of the most important elements. Cadmium pollution in soil and water bodies mainly comes from industrial projects introduced by human activities, such as mining, leather processing, metal smelting and fertilizer production.

[0003] The main methods for remediating cadmium in soil and water are physical remediation, chemical remediation and biological remediation. In contrast, biological remediation is an emerging remediation method with the advantages of simple operation, low cost, green and pollution-free. At present, the microbial strains used to remediate cadmium-containing soil or water have poor tolerance to heavy metals such as copper, zinc, and cadmium and poor remediation effect. Summary of the invention

[0004] The embodiments of the present application provide a strain and its application to solve the technical problem that the microbial strains currently used to repair cadmium-containing soil or water have poor tolerance to heavy metals such as copper, zinc, and cadmium and poor repair effect.

[0005] In the first aspect, an embodiment of the present application provides a strain, which belongs to the genus Bacillus and is named Bacillus sp. B3. The strain was deposited in the China Center for Type Culture Collection on June 8, 2022, with the deposit address being Wuhan University, Wuhan City, Hubei Province, and the deposit number being CCTCC NO: M 2022831.

[0006] Furthermore, the strain has any one of the following 16S rDNA nucleotide sequences:

[0007] Ⅰ. having the nucleotide sequence shown in SEQ ID NO: 1;

[0008] II. The nucleotide sequence shown in SEQ ID NO: 1 is modified, substituted, deleted or one or more bases are added to obtain

[0009] The obtained nucleotide sequence;

[0010] III. a sequence having at least 80% homology to the nucleotide sequence shown in SEQ ID NO: 1;

[0011] IV. The complementary sequence of the sequence shown as Ⅰ, Ⅱ or III.

[0012] In a second aspect, an embodiment of the present application provides an application of the strain described in the first aspect in remediating a cadmium-contaminated medium, wherein the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0013] In a third aspect, an embodiment of the present application provides an application of the strain described in the first aspect in removing cadmium from a cadmium-contaminated medium, wherein the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0014] In a fourth aspect, an embodiment of the present application provides the use of the strain described in the first aspect in increasing the pH of a culture medium, wherein the culture medium comprises one of acidic soil and acidic water.

[0015] In a fifth aspect, an embodiment of the present application provides a method for repairing a cadmium-contaminated medium, the method comprising:

[0016] The strain described in the first aspect is activated, expanded and cultured, and then inoculated into a cadmium-contaminated medium; wherein the cadmium-contaminated medium includes at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0017] In a sixth aspect, an embodiment of the present application provides a method for increasing the pH of a culture medium, the method comprising:

[0018] The strain described in the first aspect is activated, expanded and cultured, and then inoculated into a culture medium; wherein the culture medium comprises one of acidic soil and acidic water.

[0019] In the seventh aspect, an embodiment of the present application provides a preparation for repairing a cadmium-contaminated medium, the preparation comprising the strain described in the first aspect; the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0020] In an eighth aspect, an embodiment of the present application provides a cadmium removal agent, which includes the strain described in the first aspect.

[0021] In a ninth aspect, an embodiment of the present application provides a pH-increasing agent, wherein the pH-increasing agent comprises the strain described in the first aspect.

[0022] The above technical solution provided by the embodiment of the present application has the following advantages compared with the prior art:

[0023] The embodiment of the present application provides a strain, the growth condition of which is basically unaffected when facing cadmium exposure, and it can not only cope with high concentrations of cadmium stress (MIC is 350mg / L); but also has good tolerance to heavy metals copper and zinc, with MICs of 800mg / L and 550mg / L, respectively. In addition, the strain can also significantly change the pH of the culture medium. The clearance experiment shows that the strain can remove most of the cadmium in LB medium and basically remove all cadmium in 0.85% NaCl. It is suitable for the restoration of contaminated water bodies, and effectively solves the technical problems that the microbial strains currently used to repair cadmium-containing soil or water bodies have poor tolerance to heavy metals such as copper, zinc, and cadmium and poor restoration effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0026] Figure 1 The morphology of Bacillus sp. B3 on solid culture medium provided in the examples of the present application;

[0027] Figure 2 The Gram staining image of Bacillus sp. B3 provided in the examples of the present application;

[0028] Figure 3 The morphology of Bacillus sp. B3 under a transmission electron microscope provided in the examples of the present application;

[0029] Figure 4 A phylogenetic tree of Bacillus sp. B3 provided in the examples of the present application;

[0030] Figure 5 The growth curve of Bacillus sp. B3 at different cadmium concentrations provided in the examples of the present application;

[0031] Figure 6 is the MIC of cadmium to Bacillus sp. B3 provided in the examples of the present application;

[0032] Figure 7is the MIC of copper to Bacillus sp. B3 provided in the examples of the present application;

[0033] Figure 8 is the MIC of zinc to Bacillus sp. B3 provided in the examples of the present application;

[0034] Fig. 9 The pH change of Bacillus sp. B3 in LB medium containing different cadmium concentrations over time provided in the examples of the present application;

[0035] Fig.10 The cadmium removal rate of Bacillus sp. B3 in LB medium provided in the examples of the present application;

[0036] Fig.11 The cadmium removal rate of Bacillus sp. B3 in 0.85% NaCl provided in the examples of this application;

[0037] Fig.12 The cadmium removal rate of Bacillus sp. B3 in 0.85% NaCl (pH=5) provided in the examples of the present application. DETAILED DESCRIPTION

[0038] The present invention will be described in detail below in conjunction with specific implementations and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific implementations and examples are for illustrating the present invention, rather than limiting the present invention.

[0039] Throughout the specification, unless otherwise specifically stated, the terms used herein should be understood as meanings commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art to which the present invention belongs. In the event of a conflict, the present specification takes precedence.

[0040] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0041] With the rapid development of modern industrialization, soil and water bodies are polluted by heavy metals, among which cadmium is one of the most important elements. Cadmium pollution in soil and water bodies mainly comes from industrial projects introduced by human activities, such as mining, leather processing, metal smelting and fertilizer production.

[0042] The main methods for remediating cadmium in soil and water are physical remediation, chemical remediation and biological remediation. In contrast, biological remediation is an emerging remediation method with the advantages of simple operation, low cost, green and pollution-free. At present, the microbial strains used to remediate cadmium-containing soil or water have poor tolerance to heavy metals such as copper, zinc, and cadmium and poor remediation effect.

[0043] The technical solution provided by the embodiment of the present invention is to solve the above technical problems, and the overall idea is as follows:

[0044] In the first aspect, an embodiment of the present application provides a strain, which belongs to the genus Bacillus and is named Bacillus sp. B3. The strain was deposited in the China Center for Type Culture Collection on June 8, 2022, with the deposit address being Wuhan University, Wuhan City, Hubei Province, and the deposit number being CCTCC NO: M 2022831.

[0045] The present application embodiment provides a strain, which belongs to the genus Bacillus and is named Bacillus sp. B3. The strain was deposited in the China Center for Type Culture Collection on June 8, 2022, and the deposit address is Wuhan University, Wuhan City, Hubei Province, and the deposit number is CCTCC NO: M 2022831. The growth of this strain is basically unaffected by cadmium exposure. It can not only cope with high concentrations of cadmium stress (MIC is 350 mg / L); but also has good tolerance to heavy metals copper and zinc, with MICs of 800 mg / L and 550 mg / L, respectively. In addition, the strain can also significantly change the pH of the culture medium. The clearance experiment shows that the strain can remove most of the cadmium in LB medium, and basically remove all cadmium in 0.85% NaCl, which is suitable for the remediation of contaminated water bodies, and effectively solves the technical problems that the microbial strains currently used to repair cadmium-containing soil or water bodies have poor tolerance to heavy metals such as copper, zinc, and cadmium and poor remediation effect.

[0046] As an implementation of the present application, the strain has any one of the following 16S rDNA nucleotide sequences:

[0047] Ⅰ. having the nucleotide sequence shown in SEQ ID NO: 1;

[0048] II. The nucleotide sequence shown in SEQ ID NO: 1 is modified, substituted, deleted or one or more bases are added to obtain

[0049] The obtained nucleotide sequence;

[0050] III. a sequence having at least 80% homology to the nucleotide sequence shown in SEQ ID NO: 1;

[0051] IV. The complementary sequence of the sequence shown as Ⅰ, Ⅱ or III.

[0052] In a second aspect, an embodiment of the present application provides an application of the strain described in the first aspect in remediating a cadmium-contaminated medium, wherein the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0053] In the present application, the strain described in the first aspect can remove up to 95.32% of cadmium ions under oligotrophic conditions, thereby removing cadmium from cadmium-contaminated media such as cadmium-contaminated soil and cadmium-contaminated water, thereby achieving the repair and improvement of cadmium-contaminated media.

[0054] In a third aspect, an embodiment of the present application provides an application of the strain described in the first aspect in removing cadmium from a cadmium-contaminated medium, wherein the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0055] In the present application, the growth of the strain is basically unaffected in the presence of 100 mg / L CdCl2·2.5H2O, and it can not only cope with a higher concentration of cadmium stress (MIC is 350 mg / L); but also has good tolerance to heavy metals copper and zinc, with MICs of 800 mg / L and 550 mg / L, respectively. Experiments show that the strain can remove most of the cadmium in LB culture medium and basically remove all of the cadmium in 0.85% NaCl, and can be used to remove cadmium in cadmium-contaminated media such as cadmium-contaminated soil and cadmium-contaminated water.

[0056] In a fourth aspect, an embodiment of the present application provides the use of the strain described in the first aspect in increasing the pH of a culture medium, wherein the culture medium comprises one of acidic soil and acidic water.

[0057] In the present application, the strain can significantly increase the pH of the culture medium, which helps to solve cadmium pollution and improve acidic soil or water.

[0058] In a fifth aspect, an embodiment of the present application provides a method for repairing a cadmium-contaminated medium, the method comprising:

[0059] The strain described in the first aspect is activated, expanded and cultured, and then inoculated into a cadmium-contaminated medium; wherein the cadmium-contaminated medium includes at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0060] In the present application, the inoculation amount of the strain in the method can be selected according to the amount of cadmium-contaminated medium to be repaired and according to the microbial remediation technology reported in the prior art, and this application document will not repeat it.

[0061] In a sixth aspect, an embodiment of the present application provides a method for increasing the pH of a culture medium, the method comprising:

[0062] The strain described in the first aspect is activated, expanded and cultured, and then inoculated into a culture medium; wherein the culture medium comprises one of acidic soil and acidic water.

[0063] In the seventh aspect, an embodiment of the present application provides a preparation for repairing a cadmium-contaminated medium, the preparation comprising the strain described in the first aspect; the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

[0064] In some specific embodiments, the preparation may include some auxiliary materials in addition to the strain described in the first aspect. The general requirements of the auxiliary materials are that they are stable in nature, have no incompatibility with the strain, do not produce side effects, and do not affect their effects.

[0065] In an eighth aspect, an embodiment of the present application provides a cadmium removal agent, which includes the strain described in the first aspect.

[0066] In some specific embodiments, the preparation may include some auxiliary materials in addition to the strain described in the first aspect. The general requirements of the auxiliary materials are that they are stable in nature, have no incompatibility with the strain, do not produce side effects, and do not affect their effects.

[0067] In a ninth aspect, an embodiment of the present application provides a pH-increasing agent, wherein the pH-increasing agent comprises the strain described in the first aspect.

[0068] In some specific embodiments, the preparation may include some auxiliary materials in addition to the strain described in the first aspect. The general requirements of the auxiliary materials are that they are stable in nature, have no incompatibility with the strain, do not produce side effects, and do not affect their effects. The main function of the above-mentioned pH-increasing agent includes increasing the pH of the culture medium, and can be applied to acidic soils and acidic water bodies, etc.

[0069] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only intended to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then proceed according to general international standards, conventional conditions, or according to the conditions recommended by the manufacturer.

[0070] Example 1: Screening and separation of strains

[0071] 1.1 Water sample collection

[0072] Water samples were collected from the farmland of the Guangxi University Rice Experimental Base and marked.

[0073] 1.2 Isolation of cadmium-resistant microorganisms

[0074] The collected water samples were diluted 10 times to 10 -5 Then take an appropriate amount of diluted water sample and evenly spread it on the LB plate containing 100mg / L CdCl2·2.5H2O, and invert it to culture overnight. Then select larger and different morphological single colonies and transfer them to the LB plate containing 200mg / L CdCl2·2.5H2O, and invert it to culture overnight. 2+ Repeat this step until a single colony that grows well on the LB plate containing 1000 mg / L CdCl2·2.5H2O is screened. Pick a single clone of each type and culture it in a 1.5 mL centrifuge tube, culture it at 200 r / min and 37°C, purify it 3-4 times by plate streaking method (until the colony morphology under the microscope is consistent, indicating that the purification is complete), add 15% sterile glycerol and store it at -80°C for use.

[0075] Results Eight bacterial strains were isolated from the water samples, numbered 1 to 8, and these were the isolated and purified strains to be tested.

[0076] 1.3 Screening of cadmium-resistant strains

[0077] Eight strains to be tested were inoculated with the same inoculum into LB liquid culture medium containing cadmium. After culturing for 48 hours, the remaining cadmium content in the culture medium was detected by ICP-MS to screen out the strain with the best resistance as the target strain for study.

[0078] The results showed that strain No. 3 was finally found to have strong tolerance and good removal effect.

[0079] Example 2: Identification of strains

[0080] 2.1 Morphological identification of strains

[0081] The strain B3 was streaked on LB solid medium by the plate streak method, and the single colony status was observed after inverted overnight culture at 37°C, mainly including colony size, color, colony surface status and edge status. On the other hand, a certain amount of bacteria was taken and smeared and then observed under a microscope for bacterial morphology.

[0082] On the LB plate, strain B3 forms approximately round, smooth, protruding, light yellow colonies, which are short rods under the microscope and purple in Gram staining, belonging to Gram-positive bacteria. Flagella can also be observed under a transmission electron microscope, which are about 2 μm long and 0.5 μm wide (see Figure 1 , Figure 2 and Figure 3 ).

[0083] 2.2 16S rDNA sequence and phylogenetic analysis

[0084] The strain was inoculated into LB medium and cultured at 37°C and 200 r / min for 12 hours. The total DNA of B3 was extracted using a bacterial total DNA extraction kit. 16S rDNA was amplified by PCR and sent to a biological company for sequencing. The obtained strain sequence was subjected to Blast sequence comparison analysis in the NCBI database, and a phylogenetic tree was constructed using MEGA7.0 (see Figure 4 ).

[0085] The sequencing results showed that B3 was Bacillus sp., and its 16S rDNA sequence was as shown in SEQ ID

[0086] NO: 1; and the Bacillus sp. strain was named Bacillus sp. B3 (Bacillus sp.

[0087] B3), deposited in the China Center for Type Culture Collection on June 8, 2022, with the deposit address being Wuhan University, Wuhan City, Hubei Province, and the deposit number being CCTCC NO: M 2022831.

[0088] Example 3: Growth curves of B3 at different cadmium concentrations

[0089] Take the overnight cultured B3 solution and inoculate it into 5 mL of fresh LB medium containing 0, 1, 10, and 100 mg / L CdCl2·2.5H2O at a 1% inoculum. Cultivate at 37°C and 200 rpm / min. Take samples and measure OD at 6, 12, 24, 36, 48, 60, and 72 hours. 600 The absorbance value at nm was repeated 3 times for each group of experiments. 600 The absorbance value at nm is plotted on the Y axis to draw a growth curve (see Figure 5 ).

[0090] Example 4: Minimum inhibitory concentrations of cadmium, copper and zinc against strain B3

[0091] (1) Take the culture until OD 600 =0.5 of B3 bacterial solution was inoculated into fresh LB liquid culture medium at an inoculum size of 1%.

[0092] (2) The inoculum in (1) was transferred to a 96-well plate, 180 μl per well, and then 20 μl of different concentrations of CdCl2·2.5H2O / CuCl2 / ZnCl2 were added to make the final concentrations 0, 100, 200, 400, 600, 800, 900, and 10000 mg / L, of which 0 mg / L was the positive control and LB liquid culture medium alone was the negative control. Each group was repeated 3 times in parallel.

[0093] (3) The concentration with an inhibition rate of 90% is considered to be the minimum inhibitory concentration of the heavy metal to strain B3 (see Figure 6-8 ).

[0094] Example 5: Removal of Cadmium by Strain B3 in LB and 0.85% NaCl

[0095] 5.1 Removal of cadmium by strain B3 in LB

[0096] Take the overnight cultured B3 bacterial solution and inoculate it into 5 mL of fresh LB medium containing 0, 1, and 10 mg / L CdCl2·2.5H2O at a 1% inoculum. Incubate at 37°C, 200 rpm / min. Take samples at 0, 24, 48, and 72 h, centrifuge at 4000×g for 5 min, and take the supernatant. Digest with a graphene digester and dilute to an appropriate concentration. Measure the remaining cadmium content in the culture medium using ICP-MS. Each group of experiments was repeated 3 times (see Fig.10 ).

[0097] 5.2 Removal of cadmium by strain B3 in 0.85% NaCl

[0098] Take the overnight cultured B3 bacterial solution, centrifuge at 4000×g for 5 min, discard the supernatant, wash three times with 0.85% NaCl, and resuspend in 0.85% NaCl to make the OD 600 =0.5. Then add different concentrations but equal amounts of CdCl2·2.5H2O to make the concentrations of 0, 1, and 10 mg / L per 5 mL. Incubate at 37°C and 200 rpm / min. Take samples at 0, 24, 48, and 72 h, centrifuge at 4000×g for 5 min, and take the supernatant. Digest with a graphene digester and dilute to an appropriate concentration. Use ICP-MS to measure the remaining cadmium content in the culture medium. Each group of experiments was repeated 3 times (see Fig.11 ).

[0099] 5.3 Calculation of clearance rate

[0100] C=(C1-C2) / C1

[0101] C: clearance rate; C1: cadmium content in the culture medium at 0h; C2: remaining cadmium content in the culture medium

[0102] Example 6: Effect of strain B3 on pH of culture medium

[0103] Take the overnight cultured B3 bacterial solution and inoculate 1% of it into 5 mL of fresh LB medium containing 0, 1, 10, and mg / L CdCl2·2.5H2O. Incubate at 37°C, 200 rpm / min. Take samples at 0, 12, 24, 36, 48, and 60 h, centrifuge at 4000×g for 5 min, and take the supernatant. Use Macherey-Nagel precision pH test paper to measure the pH of the culture supernatant (see Fig. 9 ).

[0104] Example 7: Effect of low pH on cadmium removal by B3

[0105] Take the overnight cultured B3 bacterial solution, centrifuge at 4000×g for 5 min, discard the supernatant, wash three times with 0.85% NaCl, and resuspend in 0.85% NaCl (pH adjusted to 5 with hydrochloric acid) to make OD 600 =0.5. Then add CdCl2·2.5H2O to a concentration of 1 mg / L. Cultivate at 37°C, 200 rpm / min. Take samples at 24, 48, and 72 h, centrifuge at 4000×g for 5 min, and take the supernatant. Digest with a graphene digester and dilute to an appropriate concentration. Measure the remaining cadmium content in the culture medium using ICP-MS. Repeat 3 times (see Fig.12 ). The above experimental results show that the embodiment of the present application provides a strain, the growth condition of which is basically unaffected when facing cadmium exposure, and it can not only cope with high concentrations of cadmium stress (MIC is 350mg / L); but also has good tolerance to heavy metals copper and zinc, with MICs of 800mg / L and 550mg / L, respectively. In addition, the strain can also significantly increase the pH of the culture medium. The clearance experiment shows that the strain can remove most of the cadmium in LB medium, and can remove up to 95.32% of cadmium ions under oligotrophic conditions, and can basically remove all cadmium in 0.85% NaCl. It is suitable for the repair of contaminated water bodies, and effectively solves the technical problems that the microbial strains currently used to repair cadmium-containing soil or water bodies have poor tolerance to heavy metals such as copper, zinc, and cadmium and poor repair effects.

[0106] It should be understood that the endpoints and any values ​​of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0107] It should be noted that, in this article, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In addition, the term "and / or" appearing in this article is only a kind of association relationship describing associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone.

[0108] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A strain, characterized in that The strain belongs to the genus Bacillus and is named B3. The strain was deposited in the China Center for Type Culture Collection on June 8, 2022, with the preservation address being Wuhan University, Wuhan City, Hubei Province, and the preservation number being CCTCCNO: M2022831.

2. The use of the strain according to claim 1 in repairing cadmium-contaminated media, characterized in that: The cadmium-contaminated medium includes at least one of cadmium-contaminated soil and cadmium-contaminated water.

3. The use of the strain according to claim 1 in removing cadmium from cadmium-contaminated media, characterized in that: The cadmium-contaminated medium includes at least one of cadmium-contaminated soil and cadmium-contaminated water.

4. Use of the strain according to claim 1 in increasing the pH of a culture medium, characterized in that: The culture medium includes one of acidic soil and acidic water.

5. A method for repairing a cadmium-contaminated medium, characterized in that: The method comprises: The strain according to claim 1 is activated, expanded and cultured, and then inoculated into a cadmium-contaminated medium; wherein the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

6. A method for increasing the pH of a culture medium, characterized in that: The method comprises: The strain according to claim 1 is activated, expanded and cultured, and then inoculated into a culture medium; wherein the culture medium comprises one of acidic soil and acidic water.

7. A preparation for repairing cadmium-contaminated media, characterized in that: The preparation comprises the strain according to claim 1; and the cadmium-contaminated medium comprises at least one of cadmium-contaminated soil and cadmium-contaminated water.

8. A cadmium removal agent, characterized in that: The cadmium removing agent comprises the strain according to claim 1.

9. A pH increasing agent, characterized in that The pH-increasing agent comprises the strain according to claim 1.

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