Aspartase mutants and their use in r-3-aminobutyric acid synthesis

By modifying the aspartic acid enzyme mutant and using high-density fermentation technology, and optimizing catalytic conditions, the problems of long production time and high cost of R-3-aminobutyric acid in existing technologies have been solved, and efficient and low-cost industrial production has been achieved.

CN115976003BActive Publication Date: 2026-06-12MEIBANG MEIHE BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
MEIBANG MEIHE BIOTECHNOLOGY CO LTD
Filing Date
2022-10-31
Publication Date
2026-06-12

AI Technical Summary

⚠Technical Problem

Existing chemical synthesis and bioenzymatic methods for producing R-3-aminobutyric acid suffer from problems such as long reaction times, high costs, severe pollution, and low conversion rates, making it difficult to achieve large-scale industrialization.

⚗Method used

By designing aspartic acid mutants through molecular modification, combining high-density fermentation technology, and optimizing catalytic conditions, a new bio-enzymatic method for the production of R-3-aminobutyric acid was developed. The modified aspartic acid mutants can efficiently catalyze the synthesis of R-3-aminobutyric acid in a short time.

🎯Benefits of technology

It achieves high conversion and high selectivity in the synthesis of R-3-aminobutyric acid, shortens the reaction time, reduces production costs, and is suitable for large-scale industrial production.

✦ Generated by Eureka AI based on patent content.
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Abstract

The application provides an aspartase mutant and a method for producing R-3-aminobutyric acid by using the aspartase mutant, and belongs to the field of enzyme engineering. The amino acid sequence of the mutant is obtained by one or more times of mutation of the sequence shown in SEQ ID NO. 3. The mutant contains one or more of the four mutant sites at positions 237 (T237C), 31 (I31V), 88 (K88N) and 123 (G123S). The aspartase mutant of the application has the advantages of high catalytic activity and high selectivity. The cell obtained by fermentation can catalyze 300 g / L of substrate, which is the highest substrate concentration reported at present. When the aspartase mutant is used for synthesizing R-3-aminobutyric acid, the R-3-aminobutyric acid production rate and the chiral purity can reach more than 99% in 7 hours. Compared with the previous chemical synthesis method and biological enzyme method, the process is simple, the conversion rate is high, the production cost is relatively low, and the aspartase mutant has a good industrial application prospect.
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