A water-oil compartment emulsified soothing and repairing facial mask liquid, preparation method thereof, and facial mask

Abstract

The present invention discloses a water-oil compartment emulsified soothing and repairing facial mask liquid, a preparation method thereof and a facial mask. The water-oil compartment emulsified soothing and repairing facial mask liquid is stored in water phase and oil phase compartments, and the water phase is composed of the following components: sage seed extract, hops extract, spathiphyllum extract, water front temple laver polysaccharide, penetration aid, skin conditioner, other auxiliary agent A; water; the oil compartment is composed of the following components: sunflower seed oil unsaponifiable matter, emulsifier, other auxiliary agent B, squalane. The facial mask liquid prepared in this application can effectively improve the effect of anti-5-LOX activity and protect skin cells, reduce the damage of UVB irradiation to skin keratin, effectively resist external environmental stimulation, and enhance skin barrier ability.

Description

Technical Field

[0001] The invention relates to the field of skin care products, and in particular to a water-oil separated emulsified soothing and repairing facial mask liquid, a preparation method thereof, and a facial mask. Background Art

[0002] The skin barrier is composed of the sebum membrane and stratum corneum, which can protect the moisture in the skin and prevent the evaporation of moisture in the body. At the same time, it can effectively protect our skin from being harmed when facing the stimulation of harmful substances from the outside. However, when the skin barrier is damaged, the barrier is broken, the fragile skin will be directly exposed to stimulation, and harmful substances can easily penetrate the stratum corneum, causing the activation of inflammatory factors.

[0003] In daily work and life, a large amount of UV radiation in the external environment will cause excessive ROS to be produced in the skin, thereby causing oxidative stimulation. ROS easily reacts with polyunsaturated fatty acids to form a series of lipid peroxides such as MDA and 4-HNE, which cross-link with proteins, nucleic acids, etc. to produce insoluble substances, damaging cell membrane function. Therefore, UV radiation damage is one of the main causes of skin barrier damage.

[0004] The mask can seal the skin, block the air, and achieve the effect of penetrating the skin. It can make the active ingredients blend into the skin and take care of the skin quickly and effectively. How to combine the penetration effect of the mask to develop a mask that effectively resists the stimulation of the external environment and has a better anti-inflammatory effect to achieve the effect of soothing and repairing the skin still has room for improvement. Summary of the invention

[0005] In order to develop a facial mask that can effectively resist external environmental stimulation and enhance the skin barrier ability, the present application provides a water-oil separated emulsified soothing and repairing facial mask liquid, a preparation method thereof, and a facial mask.

[0006] A water-oil emulsified soothing and repairing facial mask liquid is stored in separate compartments of a water phase and an oil phase, the mass ratio of the water phase to the oil phase is 4-6:1, and the water phase is composed of the following components in percentage by mass:

[0007] Chia seed extract 0.8-2.5%

[0008] Hops extract 0.8-2.5%

[0009] Lithospermum officinale extract 2-4%

[0010] Shuiqiansi Laver Polysaccharide 0.5-1.5%

[0011] Penetration aid 2.08-4.16%

[0012] Skin Conditioner 0.3-1.32%

[0013] Other additives A 5.29-14.88%;

[0014] Add water to the water tank until the total mass percentage is 100%;

[0015] The oil tank is composed of the following components in percentage by mass:

[0016] Sunflower seed oil unsaponifiable matter 1-3%

[0017] Emulsifier 2-3%

[0018] Other additives B 21.3-32.7%

[0019] Squalane was added to the oil tank to a total mass percentage of 100%.

[0020] The water-soluble active ingredients sage extract, hops extract, Lithops spurpurogenum extract and Mizuzenji Porphyra polysaccharide and the oil-soluble active ingredient sunflower seed oil unsaponifiable matter are stored separately through the compartment method. These active ingredients maintain maximum activity during storage. The combination of these components can synergistically help the skin resist UVB damage in the external environment. At the same time, these active ingredient combinations can also help repair cell damage in the skin, and the synergistic inhibition of the activity of 5-lipoxygenase (5-LOX) is excellent, effectively reducing inflammation and soothing the skin, with better anti-inflammatory effects.

[0021] Preferably, the penetration aid is composed of tetrahydropiperine and methylpropanediol, the mass percentage of the tetrahydropiperine in the water-oil emulsified soothing and repairing facial mask liquid is 0.08-0.16%, and the mass percentage of the methylpropanediol in the water-oil emulsified soothing and repairing facial mask liquid is 2-4%.

[0022] By adding tetrahydropiperine and methylpropanediol in the above proportion, the penetration effect and mixing uniformity of sage extract, hops extract, Lithops sphaerocephala extract, Porphyra aquatica polysaccharide and sunflower seed oil unsaponifiable matter can be improved, the absorption of the above active ingredients can be accelerated, and the above active ingredients can be promoted to exert better synergistic resistance to UVB damage and anti-inflammatory effects in the skin.

[0023] Preferably, the skin conditioner comprises 0.3-0.8% by mass of lactobacillus fermentation lysate.

[0024] Adding lactobacillus fermentation lysate in combination with the above-mentioned active ingredients is beneficial to enhancing the repairing effect of the mask liquid, and is beneficial to inhibiting 5-LOX, improving the repairing and anti-inflammatory effects of the mask liquid, and can also improve the skin's resistance to UVB damage to a certain extent.

[0025] Preferably, the skin conditioner further comprises the following components in percentage by weight:

[0026] Lotus water 0.3-0.7%

[0027] Broom leaf Australian tea extract 0.4-0.6%

[0028] Stephania tetrandra extract 0.01-0.03%.

[0029] The addition of lotus water, broom leaf Australian tea extract and stephania tetrandra extract can help improve the repair effect of the mask liquid and help relieve skin discomfort.

[0030] Preferably, the other auxiliary agent A includes one or more of a thickener, a neutralizer, a humectant and a preservative.

[0031] Preferably, the thickener is acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, and the mass percentage of the thickener is 0.1-0.2%.

[0032] The neutralizing agent is tromethamine, and the mass percentage of the neutralizing agent is 0.05-0.15%.

[0033] The moisturizing agent is one or more of diglycerin, acetylated sodium hyaluronate, 1,3-butylene glycol, sodium hyaluronate, and betaine. Further, the moisturizing agent is composed of the following components in mass percentage: 1-3% diglycerin, 0.03-0.08% acetylated sodium hyaluronate, 2-5% 1,3-butylene glycol, 0.01-0.05% sodium hyaluronate, 1-3% betaine, and 0.3-0.6% 1,2-hexanediol.

[0034] The preservative is p-hydroxyacetophenone, and the mass percentage of the preservative is 0.3-0.6%.

[0035] Preferably, the emulsifier is polysorbate-85.

[0036] The combination of emulsifier polysorbate-85 and thickener acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer is conducive to the rapid and uniform mixing of the water tank and the oil tank, so that the components in the water phase and the oil phase are fully mixed and dispersed, resulting in better interaction and exerting excellent synergistic anti-UVB damage and synergistic inhibition of 5-LOX.

[0037] Preferably, the other auxiliary agent B is one or more of jojoba seed oil, white meadowfoam seed oil and tocopherol acetate. Further, the other auxiliary agent B is composed of the following components in mass percentage: jojoba seed oil 18-24%, white meadowfoam seed oil 3-8% and tocopherol acetate 0.3-0.7%.

[0038] A method for preparing a water-oil compartment emulsified soothing and repairing facial mask liquid comprises the following steps:

[0039] Preparation of aqueous phase: Mix water, permeation aid and other auxiliary agent A evenly, then add pre-mixed Spanish chia seed extract, lactobacillus fermentation lysate, hops extract, Lithops spurredum extract, Porphyra mizuna polysaccharide and skin conditioner, stir evenly to obtain aqueous phase;

[0040] Preparation of oil phase: Mix all raw materials of oil phase, stir evenly to obtain oil phase.

[0041] The utility model discloses a water-oil separation emulsified soothing and repairing facial mask, which is composed of a water-oil separation emulsified soothing and repairing facial mask liquid and a mask cloth.

[0042] When storing the mask, the oil phase, water phase and membrane cloth are stored separately. Compared with ordinary emulsion masks, it can reduce the amount of preservatives, reduce irritation and prevent the membrane cloth from reacting with the mask liquid and deforming. When using, squeeze the oil phase from the oil tank into the water phase and press to mix, then squeeze it into the membrane cloth and mix thoroughly before use.

[0043] In summary, this application has the following beneficial effects:

[0044] 1. The present application found that the water-soluble active ingredients of sage extract, hops extract, Lithops spurpurogenum extract and Porphyra aquatica polysaccharide and the oil-soluble active ingredient of sunflower seed oil unsaponifiable matter can act synergistically to effectively enhance the anti-5-LOX activity and protect skin cells, and reduce the damage of UVB irradiation to skin keratin.

[0045] 2. The addition of lotus water, broom leaf Australian tea extract and stephania tetrandra extract can help improve the repair effect of the mask liquid, help relieve skin discomfort, and is more effective in reducing the damage of UVB radiation to skin keratin. DETAILED DESCRIPTION

[0046] All raw materials in this application can be obtained commercially, among which Spanish sage (SALVIA HISPANICA) seed extract, lactobacillus fermentation lysate, hops (HUMULUSLUPULUS) extract, and Lithothamnion Calcareum extract were purchased from Guangzhou Noran Biotechnology Co., Ltd., and lotus (NELUMBONUCIFERA) flower water, LEPTOSPERMUM SCOPARIUM leaf extract, and Stephania tetrandra (STEPHANIATE TRANDRA) extract were purchased from Guangzhou Xiexie Biotechnology Co., Ltd.

[0047] Example 1

[0048] A water-oil emulsified soothing and repairing facial mask liquid comprises a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 4:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0049] Preparation of aqueous phase: 1 g of diglycerol, 0.03 g of acetylated sodium hyaluronate, 2 g of 1,3-butylene glycol, 0.01 g of sodium hyaluronate, 1 g of betaine, 0.08 g of tetrahydropiperine, 0.1 g of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.3 g of 1,2-hexanediol and 89.03 g of water were put into an emulsifier, heated to 80°C, homogenized for 5 min, and kept warm for 10 min to obtain a phase A mixture; the phase A mixture was cooled to 45°C and 0.05 g tromethamine, continuously stirring to obtain a phase B mixture; mix 0.3 g p-hydroxyacetophenone and 2 g methylpropanediol, heat to 60°C, and stir until dissolved evenly to obtain a phase C mixture; mix 0.8 g Spanish sage seed extract, 0.8 g hops extract, 2 g Lithospermum officinale extract and 0.5 g Porphyra aquatica polysaccharide, stir evenly to obtain a phase D mixture, wait until the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir evenly to obtain an aqueous phase.

[0050] Preparation of oil phase: add 1g of unsaponifiable matter of sunflower seed oil, 2g of polysorbate-85, 18g of jojoba seed oil, 3g of meadowfoam seed oil, 0.3g of tocopherol acetate, and 75.7g of squalane into a drying emulsifier, stir evenly to obtain an oil phase.

[0051] Example 2

[0052] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0053] Preparation of aqueous phase: 2 g of diglycerol, 0.05 g of acetylated sodium hyaluronate, 3.5 g of 1,3-butylene glycol, 0.025 g of sodium hyaluronate, 2 g of betaine, 0.1 g of tetrahydropiperine, 0.15 g of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45 g of 1,2-hexanediol and 82.175 g of water were put into an emulsifier, heated to 80°C, homogenized for 5 min, and kept warm for 10 min to obtain a phase A mixture; the phase A mixture was cooled to 45°C and added Add 0.1g of tromethamine, and continue stirring to obtain a phase B mixture; mix 0.45g of p-hydroxyacetophenone and 3g of methylpropanediol, heat to 65°C, and stir until dissolved evenly to obtain a phase C mixture; mix 1g of Spanish sage seed extract, 1g of hops extract, 3g of Lithops spurredum extract and 1g of Porphyra aquatica polysaccharide, and stir evenly to obtain a phase D mixture; wait until the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir evenly to obtain an aqueous phase.

[0054] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0055] Example 3

[0056] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 6:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0057] Preparation of aqueous phase: 3g diglycerin, 0.08g acetylated sodium hyaluronate, 5g 1,3-butylene glycol, 0.05g sodium hyaluronate, 3g betaine, 0.16g tetrahydropiperine, 0.2g acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.6g 1,2-hexanediol and 72.66g water were put into an emulsifier, heated to 80°C, homogenized for 5min, and kept warm for 10min to obtain a phase A mixture; the phase A mixture was cooled to 45°C, and 0.15g ammonia was added. butylene glycol, continue stirring evenly to obtain a phase B mixture; mix 0.6g of p-hydroxyacetophenone and 4g of methylpropanediol, heat to 70°C, and stir until dissolved evenly to obtain a phase C mixture; and pre-mix 2.5g of Spanish sage seed extract, 2.5g of hops extract, 4g of Lithops extract and 1.5g of Porphyra aquatica polysaccharide, stir evenly to obtain a phase D mixture, wait until the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir evenly to obtain an aqueous phase.

[0058] Preparation of oil phase: add 3 g of unsaponifiable matter of sunflower seed oil, 3 g of polysorbate-85, 24 g of jojoba seed oil, 8 g of meadowfoam seed oil, 0.7 g of tocopherol acetate, and 61.3 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0059] Example 4

[0060] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0061] Preparation of aqueous phase: 2g diglycerin, 0.05g acetylated sodium hyaluronate, 3.5g 1,3-butylene glycol, 0.025g sodium hyaluronate, 2g betaine, 0.1g tetrahydropiperine, 0.15g acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45g 1,2-hexanediol and 81.675g water were put into an emulsifier, heated to 80°C, homogenized for 5min, and kept warm for 10min to obtain a phase A mixture; the phase A mixture was cooled to 45°C, and 0.1g ammonia was added. 1g of salvia officinalis seed extract, 1g of hops extract, 3g of Lithops spurredum extract, 1g of Porphyra aquatica polysaccharide and 0.5g of lactobacillus fermentation lysate were stirred evenly to obtain a phase D mixture; after the phase B mixture was cooled to 40°C, the phase C mixture and the phase D mixture were added and stirred evenly to obtain an aqueous phase;

[0062] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0063] Example 5

[0064] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0065] Preparation of aqueous phase: 2g diglycerin, 0.05g acetylated sodium hyaluronate, 3.5g 1,3-butylene glycol, 0.025g sodium hyaluronate, 2g betaine, 0.1g tetrahydropiperine, 0.15g acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45g 1,2-hexanediol and 80.655g water were put into an emulsifying pot, heated to 80°C, homogenized for 5min, and kept warm for 10min to obtain a phase A mixture; the phase A mixture was cooled to 45°C, 0.1g tromethamine was added, and the mixture was stirred continuously to obtain a phase B mixture; 0.45g of p-hydroxyacetophenone and 3g of methylpropanediol were mixed, the temperature was raised to 65°C, and the mixture was stirred until dissolved uniformly to obtain a phase C mixture; 1g of Spanish sage seed extract, 1g of hops extract, 3g of Lithops spurredum extract, 1g of Porphyra aquatica polysaccharide and 0.5g of lactobacillus fermentation lysate, 0.5g of lotus water, 0.5g of Stephania tetrandra extract and 0.02g of Australian tea extract were mixed and stirred uniformly to obtain a phase D mixture; after the phase B mixture was cooled to 40°C, the phase C mixture and the phase D mixture were added and stirred uniformly to obtain an aqueous phase;

[0066] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0067] Comparative Example 1

[0068] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0069] Preparation of aqueous phase: 2 g of diglycerol, 0.05 g of acetylated sodium hyaluronate, 3.5 g of 1,3-butylene glycol, 0.025 g of sodium hyaluronate, 2 g of betaine, 0.1 g of tetrahydropiperine, 0.15 g of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45 g of 1,2-hexanediol and 83.175 g of water were put into an emulsifier, heated to 80°C, homogenized for 5 min, and kept warm for 10 min to obtain a phase A mixture; the phase A mixture was cooled to 80°C and stirred for 10 min. The mixture was heated to 45°C, 0.1 g of tromethamine was added, and stirring was continued to obtain a phase B mixture; 0.45 g of p-hydroxyacetophenone and 3 g of methylpropanediol were mixed, the temperature was raised to 65°C, and stirring was continued until dissolved to obtain a phase C mixture; 1 g of hops extract, 3 g of Lithospermum officinale extract and 1 g of Porphyra aquatica polysaccharide were mixed, and stirring was performed to obtain a phase D mixture; after the phase B mixture was cooled to 40°C, the phase C mixture and the phase D mixture were added, and stirring was performed to obtain an aqueous phase.

[0070] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0071] Comparative Example 2

[0072] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0073] Preparation of aqueous phase: 2 g of diglycerol, 0.05 g of acetylated sodium hyaluronate, 3.5 g of 1,3-butylene glycol, 0.025 g of sodium hyaluronate, 2 g of betaine, 0.1 g of tetrahydropiperine, 0.15 g of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45 g of 1,2-hexanediol and 83.175 g of water were put into an emulsifier, heated to 80°C, homogenized for 5 min, and kept warm for 10 min to obtain a phase A mixture; the phase A mixture was cooled to 80°C. At 45°C, add 0.1g of tromethamine, and continue stirring to obtain a phase B mixture; mix 0.45g of p-hydroxyacetophenone and 3g of methylpropanediol, heat to 65°C, and stir until dissolved evenly to obtain a phase C mixture; mix 1g of Spanish sage seed extract, 3g of Lithops spurredum extract and 1g of Porphyra aquatica polysaccharide, and stir evenly to obtain a phase D mixture; when the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir evenly to obtain an aqueous phase.

[0074] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0075] Comparative Example 3

[0076] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0077] Preparation of aqueous phase: 2 g of diglycerol, 0.05 g of acetylated sodium hyaluronate, 3.5 g of 1,3-butylene glycol, 0.025 g of sodium hyaluronate, 2 g of betaine, 0.1 g of tetrahydropiperine, 0.15 g of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45 g of 1,2-hexanediol and 85.175 g of water were put into an emulsifier, heated to 80°C, homogenized for 5 min, and kept warm for 10 min to obtain a phase A mixture; the phase A mixture was cooled To 45°C, add 0.1g of tromethamine, and continue stirring to obtain a phase B mixture; mix 0.45g of p-hydroxyacetophenone and 3g of methylpropanediol, heat to 65°C, and stir until dissolved evenly to obtain a phase C mixture; mix 1g of Spanish sage seed extract, 1g of hops extract and 1g of water kelp polysaccharide, stir evenly to obtain a phase D mixture; when the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir evenly to obtain an aqueous phase.

[0078] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0079] Comparative Example 4

[0080] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0081] Preparation of aqueous phase: 2 g of diglycerol, 0.05 g of acetylated sodium hyaluronate, 3.5 g of 1,3-butylene glycol, 0.025 g of sodium hyaluronate, 2 g of betaine, 0.1 g of tetrahydropiperine, 0.15 g of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45 g of 1,2-hexanediol and 83.175 g of water were put into an emulsifier, heated to 80°C, homogenized for 5 min, and kept warm for 10 min to obtain a phase A mixture; the phase A mixture was cooled to 80°C. At 45°C, add 0.1g of tromethamine, and continue stirring to obtain a phase B mixture; mix 0.45g of p-hydroxyacetophenone and 3g of methylpropanediol, heat to 65°C, and stir until dissolved to obtain a phase C mixture; mix 1g of Spanish sage seed extract, 1g of hops extract and 3g of Lithops spurredum extract, and stir to obtain a phase D mixture; when the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir to obtain an aqueous phase.

[0082] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0083] Comparative Example 5

[0084] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0085] Preparation of aqueous phase: 2 g of diglycerol, 0.05 g of acetylated sodium hyaluronate, 3.5 g of 1,3-butylene glycol, 0.025 g of sodium hyaluronate, 2 g of betaine, 0.1 g of tetrahydropiperine, 0.15 g of acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.45 g of 1,2-hexanediol and 82.175 g of water were put into an emulsifier, heated to 80°C, homogenized for 5 min, and kept warm for 10 min to obtain a phase A mixture; the phase A mixture was cooled to 45°C and added Add 0.1g of tromethamine, and continue stirring to obtain a phase B mixture; mix 0.45g of p-hydroxyacetophenone and 3g of methylpropanediol, heat to 65°C, and stir until dissolved evenly to obtain a phase C mixture; mix 1g of Spanish sage seed extract, 1g of hops extract, 3g of Lithops spurredum extract and 1g of Porphyra aquatica polysaccharide, and stir evenly to obtain a phase D mixture; wait until the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir evenly to obtain an aqueous phase.

[0086] Preparation of oil phase: add 2.5 g polysorbate-85, 20 g jojoba seed oil, 5 g meadowfoam seed oil, 0.5 g tocopherol acetate and 72 g squalane into a drying emulsifying pot and stir evenly to obtain an oil phase.

[0087] Comparative Example 6

[0088] A water-oil compartment emulsified soothing and repairing facial mask liquid, comprising a water phase and an oil phase stored in separate compartments, the water phase and the oil phase are mixed in a mass ratio of 5:1, and the preparation methods of the water phase and the oil phase are respectively as follows:

[0089] Preparation of aqueous phase: 2g diglycerin, 0.05g acetylated sodium hyaluronate, 3.5g 1,3-butylene glycol, 0.025g sodium hyaluronate, 2g betaine, 0.1g tetrahydropiperine, 0.15g acrylates / C10-30 alkyl acrylate cross-polymer, 3g methyl propanediol and 83.425g water were put into an emulsifier, heated to 80°C, homogenized for 5min, and kept warm for 10min to obtain a phase A mixture; the phase A mixture was cooled to 45°C, 0. 1g tromethamine, continue stirring to obtain a phase B mixture; mix 0.45g p-hydroxyacetophenone and 3g methylpropanediol, heat to 65°C, and stir until dissolved evenly to obtain a phase C mixture; mix 0.2g Spanish sage seed extract, 2g hops extract, 1g Lithops extract and 2g Porphyra aquatica polysaccharide, stir evenly to obtain a phase D mixture; wait until the phase B mixture is cooled to 40°C, add the phase C mixture and the phase D mixture, stir evenly to obtain an aqueous phase.

[0090] Preparation of oil phase: add 2 g of unsaponifiable matter of sunflower seed oil, 2.5 g of polysorbate-85, 20 g of jojoba seed oil, 5 g of meadowfoam seed oil, 0.5 g of tocopherol acetate, and 70 g of squalane into a drying emulsifier, stir evenly, and obtain the oil phase.

[0091] Experiment 1

[0092] Anti-LOX enzyme test

[0093] Experimental principle: Inflammatory response is a protective immune response produced by the body against external stimuli. It can prevent pathogen invasion through nonspecific immunity or specific immunity and repair tissue cell damage. 5-lipoxygenase is a key enzyme in the reaction. 5-lipoxygenase regulates the expression of pro-inflammatory cytokines and other mediators. The release of pro-inflammatory mediators in the inflammatory response is beneficial to resist external stimuli. In addition, the metabolites generated by 5-lipoxygenase-catalyzed arachidonic acid also have pro-inflammatory effects, which can directly promote the production of cytokines 1L-1β, MCP-1, etc., and can also enhance the phagocytic function of macrophages.

[0094] The principle of this experiment is that 5-lipoxygenase first oxidizes the substrate linoleic acid to generate linoleic acid peroxide, and then linoleic acid peroxide oxidizes Fe 2+ Fe 3+ , Fe 3+ It reacts with xylenol orange to form a complex that has a certain absorption at a wavelength of 550-600nm. When a sample solution that has an inhibitory effect on 5-lipoxygenase is added to the reaction system, the activity of 5-lipoxygenase is inhibited and the absorbance changes. The inhibition rate of the sample on the enzyme can be calculated according to the formula.

[0095] Experimental method: Prepare the buffer solution Tris-HCl: weigh 3.0285g Tris, dissolve it in 225mL deionized water, and adjust it with 1mol / L hydrochloric acid solution.

[0096] (1) Prepare Tris-HCl buffer solution: weigh 3.0285 g Tris, dissolve it in 225 mL deionized water, adjust the pH to 7.0 with 1 mol / L hydrochloric acid solution, and make up to 250 mL.

[0097] (2) Preparation of FOX reagent: Measure 25 mL of deionized water and 225 mL of methanol in a 250 mL volumetric flask, add 340 μL of concentrated sulfuric acid, weigh 24.5 mg of ammonium ferrous sulfate hexahydrate and 19 mg of xylenol orange, and store at -20°C in the dark.

[0098] (3) Preparation of 5-LOX solution: Accurately weigh 10 mg of 5-LOX powder and dissolve it in 10 mL of buffer solution as the enzyme stock solution. When using, dilute it with buffer solution to obtain a 250 U / mL working solution.

[0099] (4) Preparation of linoleic acid (LA) solution: Take 10 μL LA and dissolve it in 15 mL anhydrous ethanol and store it at -20°C as LA stock solution. Dilute it 15 times with buffer solution before use.

[0100] 40 μL of enzyme dilution solution was transferred to a 96-well plate, and 40 μL of DMSO solution of each experimental sample was first added (40 μL buffer was added to the blank, and nordihydroguaiaretic acid (NDGA) was added to the positive group). Each sample was placed in 3 parallel wells, shaken and mixed, and incubated at 25°C for 15 minutes. Then, 20 μL of linoleic acid was added to the enzyme-labeled wells, and incubated at 25°C in the dark for 20 minutes. Subsequently, 100 μL of LFOX reagent was added, and incubated at 25°C for 10 minutes. Finally, the absorbance of the system at 560 nm was measured. The test results are shown in Table 1.

[0101]

[0102] Wherein: A blank group is the absorbance without adding experimental samples (using buffer instead of experimental samples).

[0103] A blank background group is the absorbance without adding experimental samples and linoleic acid (the buffer solution is used instead of the experimental samples and linoleic acid).

[0104] Experimental group A is the absorbance of the added experimental samples.

[0105] A experimental background group is the absorbance of the experimental sample without adding linoleic acid (buffer is used instead of linoleic acid).

[0106] Table 1

[0107] concentration Inhibition rate of 5-LOX activity % NDGA 0.05％ 101.26±1.00 Example 1 0.5％ 93.60±2.36 Example 2 0.5％ 94.85±1.52 Example 3 0.5％ 94.15±1.68 Example 4 0.5％ 95.56±2.45 Example 5 0.5％ 96.82±1.26 Comparative Example 1 0.5％ 77.68±3.62 Comparative Example 2 0.5％ 82.26±2.57 Comparative Example 3 0.5％ 74.35±1.98 Comparative Example 4 0.5％ 76.59±1.67 Comparative Example 5 0.5％ 78.52±2.41 Comparative Example 6 0.5％ 83.14±2.13

[0108] Analyzing the test results in Table 1, the facial mask liquid of Examples 1-5 has an inhibition rate of more than 93% when the concentration is 0.5%. Compared with the positive drug NDGA, the facial mask liquid of Examples 1-5 has a stronger effect of inhibiting the activity of 5-LOX.

[0109] Compared with Example 2, Comparative Example 1 did not add Spanish sage seed extract, Comparative Example 2 did not add hops extract, Comparative Example 3 did not add Lithops spurred extract, Comparative Example 4 did not add Porphyra aquatica polysaccharide, Comparative Example 5 did not add and sunflower seed oil unsaponifiable matter, and Comparative Example 6 added Spanish sage seed extract, hops extract, Lithops spurred extract, Porphyra aquatica polysaccharide and sunflower seed oil unsaponifiable matter at the same time, but the dosage ratio of these components was different from that of Example 2. The 5-LOX activity of Comparative Examples 1-6 was significantly decreased, and was significantly lower than that of Example 2. It can be seen that Spanish sage seed extract, hops extract, Lithops spurred extract, Porphyra aquatica polysaccharide and sunflower seed oil unsaponifiable matter can play a better synergistic effect within a specific proportion range, effectively improve the effect of the mask liquid in inhibiting 5-LOX activity, and improve the anti-inflammatory effect of the mask liquid.

[0110] Experiment 2

[0111] Skin barrier test

[0112] The measurement of malondialdehyde content can reflect the lipid oxidation status, thereby indirectly reflecting the degree of cell damage. The measurement of superoxide dismutase content can reflect the ability to remove superoxide anion free radicals and protect cells from damage.

[0113] Experimental method: Human epidermal keratinocytes purchased from Shanghai Hongshun Biotechnology Co., Ltd. were inoculated in 15% DMEM medium containing 1.2 μg / mL benzopyrene, resuspended and transferred into a culture bottle, and placed in a constant temperature incubator (37°C, 5% CO2) for culture. The cultured cells were immunohistochemically identified with mouse anti-human broad-spectrum keratin, and the cells expressing keratin were stained brown-yellow as the next generation keratinocytes. The 2nd to 4th generation keratinocytes after culture were used as experimental test cells.

[0114] Experimental groups: 1. Blank experimental group without UVB irradiation and without adding the test solution; 2. Irradiation with UVB 20mJ / cm 2 , a control group without adding the test solution; 3. Irradiation with UVB 20mJ / cm 2 , and experimental groups were added with the facial mask liquids prepared in each embodiment and comparative example.

[0115] According to the experimental grouping setting, the UVB irradiation test was carried out, and then the corresponding emulsion containing the deep penetration long-acting moisturizing composition was added immediately according to the above grouping, and then cultured in a constant temperature incubator at a temperature of 37°C and a mass concentration of 5% carbon dioxide for 24 hours, and then the experimental cells after culture were taken to prepare cell homogenates, and the malondialdehyde content and superoxide dismutase content were tested by the thiobarbituric acid method and the hydroxylamine method respectively according to the kit. The test results are shown in Table 2.

[0116] Table 2

[0117] Malondialdehyde (nmol / L) Superoxide dismutase (U / mgport) Blank group 39.59±4.26 278.46±10.25 Control group 60.42±4.52 105.14.82±7.26 Example 1 35.26±2.36 215.73±3.25 Example 2 34.67±2.47 229.49±2.84 Example 3 32.49±1.25 225.52±3.65 Example 4 30.58±0.98 226.61±4.15 Example 5 26.43±1.62 232.48±2.84 Comparative Example 1 48.26±3.16 153.56±4.37 Comparative Example 2 52.16±2.56 142.76±5.68 Comparative Example 3 46.85±2.46 136.42±7.12 Comparative Example 4 55.24±1.59 129.36±5.16 Comparative Example 5 49.35±3.68 131.46±6.35 Comparative Example 6 41.65±3.42 152.18±4.36

[0118] Compared with the blank group, the malondialdehyde content of keratinocytes in the control group increased after UVB irradiation, and the difference was significant (P<0.05).

[0119] Compared with the control group, the malondialdehyde content of the keratinocytes in the experimental group decreased after UVB irradiation, and the difference was significant (P<0.05). Compared with the comparative examples 1-6, the reduction in examples 1-5 was more obvious.

[0120] By comparing the test results of Example 2 with Comparative Examples 1-5 in Table 2, it can be obtained that Example 2 simultaneously adds a specific proportion of sage seed extract, hop extract, Lithocarpus spurredus extract, water front temple laver polysaccharide and sunflower seed oil unsaponifiable matter, which can effectively reduce the content of malondialdehyde, increase the content of superoxide dismutase, better protect cells from damage, and reduce the degree of cell damage. Combined with the data comparison of Comparative Example 6, it is found that the malondialdehyde content of Comparative Example 6 is higher than that of Example 2, and the superoxide dismutase content is lower than that of Example 2 by changing the dosage ratio of sage seed extract, hop extract, Lithocarpus spurredus extract, water front temple laver polysaccharide and sunflower seed oil unsaponifiable matter, which proves that the dosage of sage seed extract, hop extract, Lithocarpus spurredus extract and water front temple laver polysaccharide needs to be controlled in a specific proportion, so that these components can better cooperate and play a better protective effect and reduce skin cell damage.

[0121] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A water-oil emulsified soothing and repairing facial mask liquid, characterized by: The water phase and the oil phase are stored in separate warehouses. The water phase and the oil phase are mixed in a mass ratio of 4-6:

1. The water phase is composed of the following components in mass percentage: Chia Seed Extract 0.8-2.5% Hops extract 0.8-2.5% Lithops sutchuenensis extract 2-4% Shuiqiansi Laver Polysaccharide 0.5-1.5% Penetration aid 2.08-4.16% Skin Conditioner 0.3-1.32% Other additives A 5.29-14.88%; Add water until the total mass percentage of the aqueous phase is 100%; The oil phase is composed of the following components in percentage by mass: Sunflower seed oil unsaponifiable matter 1-3% Emulsifier 2-3% Other additives B 21.3-32.7% Squalane was added until the total mass percentage of the oil phase was 100%.

2. The water-oil emulsified soothing and repairing facial mask liquid according to claim 1, characterized in that: The penetration aid is composed of 0.08-0.16% by mass of tetrahydropiperine and 2-4% by mass of methyl propanediol.

3. The water-oil compartment emulsified soothing and repairing facial mask liquid according to claim 1, characterized in that: The skin conditioner comprises 0.3-0.8% by mass of lactobacillus fermentation lysate.

4. The water-oil compartment emulsified soothing and repairing facial mask liquid according to claim 1, characterized in that: The skin conditioning agent also includes the following components in percentage by weight: Lotus water 0.3-0.7% Broom leaf Australian tea extract 0.4-0.6% Stephania tetrandra extract 0.01-0.03%.

5. The water-oil emulsified soothing and repairing facial mask liquid according to claim 1, characterized in that: The other auxiliary agents A include one or more of a thickener, a neutralizer, a humectant and a preservative.

6. The water-oil compartment emulsified soothing and repairing facial mask liquid according to claim 5, characterized in that: The thickener is an acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, and the mass percentage of the thickener is 0.1-0.2%.

7. The water-oil compartment emulsified soothing and repairing facial mask liquid according to claim 1, characterized in that: The emulsifier is polysorbate-85.

8. The water-oil compartment emulsified soothing and repairing facial mask liquid according to claim 1, characterized in that: The other auxiliary agent B is one or more of jojoba seed oil, meadowfoam seed oil and tocopherol acetate.

9. A method for preparing the water-oil compartment emulsified soothing and repairing facial mask liquid according to any one of claims 1 to 8, characterized in that: The following steps are involved: Preparation of aqueous phase: Mix water, permeation aid and other additives A evenly, then add the pre-mixed Spanish sage seed extract, hops extract, Lithops spurredum extract, Porphyra sutchuenensis polysaccharide and skin conditioner, stir evenly to obtain an aqueous phase; Preparation of oil phase: Mix all raw materials of oil phase, stir evenly to obtain oil phase.

10. A water-oil emulsified soothing and repairing facial mask, characterized by: The invention is composed of the water-oil separation emulsified soothing and repairing facial mask liquid and the membrane cloth as described in any one of claims 1 to 8, wherein the water-oil separation emulsified soothing and repairing facial mask liquid and the membrane cloth are stored separately.

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