A method for improving the vitality of water mustard seeds

Abstract

The invention discloses a method for improving the vitality of water mustard seeds, and relates to the field of agricultural planting. The method comprises the following steps: (1) disinfecting the water mustard seeds; (2) soaking the disinfected water mustard seeds in a soaking liquid, and performing ultrasonic treatment during the soaking process; the soaking liquid contains a mangrove seed extract, Ca 2+ and diethylaminoethanol caproate; the mangrove seeds are seeds germinated under the conditions of a salt content of 0.2%-0.3% and a pH of 8-8.5. 2+ The seeds of mustard were soaked in ethylaminoethanol caproate, which improved the seed germination rate and seedling index of mustard in slightly saline-alkali land, and was beneficial to the cultivation and promotion of mustard in slightly saline-alkali land.

Description

Technical Field

[0001] The invention relates to the field of agricultural planting, in particular to a method for improving the vitality of watercress mustard seeds. Background Art

[0002] Shuidong mustard is a new high-quality vegetable variety named after its place of origin with distinct local characteristics. It belongs to the genus Brassica juncea (L.) Coss. var. foliosa Bailey of the family Cruciferae, with a chromosome number of 2n=4x=36. It has a compound raceme, a yellow corolla, and round, brown seeds. The plant is upright, with a short stem, a shallow root system, and many fibrous roots. It is a head mustard among the large-leaf mustards, with small curly core, many petioles and small leaves, and is sweet, crisp, and has no residue, is refreshing, and has little fiber, and is deeply loved by consumers.

[0003] Mangrove (Rhizophora apiculata Bl.) is a tall tree of the Rhizophoraceae family, Rhizophora genus, 2-4 meters high, with dark brown bark, elliptical to oblong-elliptical leaves, short pointed or convex apex, broad cuneate base, red below the midrib, and slightly obvious above the lateral veins after drying; petiole is thick and light red, the peduncle of mangrove is borne in the axil of fallen leaves, shorter than the petiole, with 2 flowers, the fruit is inverted pear-shaped, slightly rough, the hypocotyl is cylindrical, slightly curved, green-purple, and the flowering and fruiting period is almost all year round. It grows in shallow sea salt marshes or swamps in bays in South China where waves are calm and silt is soft, and has strong salt and alkali resistance.

[0004] In mild saline-alkali land (salt content of 0.1%-0.3%), the seedling rate of water mustard is only 60-70%. In order to promote the planting and promotion of water mustard in mild saline-alkali land, it is necessary to develop a method for improving the vitality of water mustard seeds to improve the seedling rate of water mustard seeds in mild saline-alkali land. Summary of the invention

[0005] The purpose of the present invention is to provide a method for improving the vigor of water mustard seeds to solve the problems existing in the above-mentioned prior art. The method of the present invention can improve the germination rate and seedling index of water mustard seeds in slightly saline-alkali land, thereby facilitating the cultivation and promotion of water mustard in slightly saline-alkali land.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The present invention provides a method for improving the vitality of watercress seeds, comprising the following steps:

[0008] (1) Disinfecting water mustard seeds;

[0009] (2) soaking the sterilized water mustard seeds in a soaking solution, and performing ultrasonic treatment during the soaking process;

[0010] The soaking liquid contains mangrove seed extract, Ca 2+ and diethylaminoethanol caproate; the mangrove seeds are seeds germinated under the conditions of a salt content of 0.2%-0.3% and a pH of 8-8.5.

[0011] Furthermore, the extraction method of the mangrove seed extract is: soaking the mangrove seeds for germination under the conditions of a salt content of 0.2%-0.3% and a pH of 8-8.5, then crushing the germinated seeds, and then adding distilled water for extraction to obtain the mangrove seed extract.

[0012] Furthermore, the mass volume ratio of the germinated seeds to the distilled water is 1 g:20 mL.

[0013] Furthermore, in step (2), the sterilized water mustard seeds are subjected to ultrasonic treatment in the 6th hour of being soaked in the soaking liquid.

[0014] Furthermore, the frequency of the ultrasound is 80 kHz.

[0015] Furthermore, the ultrasound duration is 1-2 hours.

[0016] Furthermore, the seed soaking time is 10-16 hours.

[0017] Furthermore, the disinfection treatment is: soaking the watercress seeds in 75% alcohol by volume for 10 minutes.

[0018] Furthermore, the Ca in the soaking solution 2+ The concentration is 15-25mmol / L.

[0019] Furthermore, the concentration of diethylaminoethanol caproate in the soaking solution is 30-40 mg / L.

[0020] The invention utilizes substances beneficial to seed germination produced by mangrove seeds under conditions of higher salinity and higher pH to soak water mustard seeds, thereby enhancing the stress resistance of water mustard seeds, promoting seed germination in slightly saline-alkali land, and further improving the seed germination rate and seedling rate.

[0021] Ca 2+ As a second messenger, it can stabilize and maintain the cytoplasmic membrane structure, weaken the membrane lipid peroxidation reaction, protect the integrity of the membrane structure and maintain the central activity of PSII, thereby alleviating the damage caused by adversity. 2+ It can change the activity of enzymes, speed up the emergence speed and improve the seedling rate. At an appropriate concentration, diethylaminoethanol hexanoate can increase the enzyme activity of metabolic enzymes in water mustard seeds, thereby enhancing the metabolic capacity of seeds and facilitating seed germination.

[0022] The present invention discloses the following technical effects:

[0023] The present invention utilizes mangrove seed extract, Ca 2+ The seeds of water mustard were soaked in ethylaminoethanol caproate, which improved the seed germination rate and seedling index of water mustard in slightly saline-alkali land; ultrasonic treatment was used to assist in improving the effect of mangrove seed extract during the seed soaking process, which further improved the seed germination rate of water mustard. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 This is the situation of experimental group 2 in Example 2 after germination for 5 days. DETAILED DESCRIPTION

[0026] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0027] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0028] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0029] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0030] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0031] In the following examples or comparative examples, the soil used for planting was collected from the saline-alkali land of Shuidong Bay in Maoming City. After testing, the soil salinity was 0.29% and the pH was 8.5.

[0032] Seedling index = (stem diameter / plant height + root dry weight / aboveground dry weight) × whole plant dry weight; stem diameter is 1 cm above the base, and plant height is the distance from the bottom of the stem to the top of the growth point.

[0033] Example 1

[0034] (1) preparing a saline solution with a NaCl concentration of 0.2% and a pH adjusted to 8;

[0035] (2) Take 100 g of mangrove seeds, spread them flat on a tray, cover them with two layers of gauze, add 100 mL of the saline prepared in step (1), and germinate them at 25° C. for 6 days;

[0036] (3) The seeds germinated for 6 days in step (2) were taken out, the ungerminated seeds were removed, and the seeds were crushed into slurry using a grinder. Then, distilled water was added to the slurry (the volume mass ratio of distilled water to slurry was 20 mL: 1 g), and the slurry was soaked for 24 hours, and then filtered to obtain the mangrove seed extract.

[0037] Example 2

[0038] (1) According to Table 1, CaCl2 and diethylaminoethanol caproate were added to the mangrove seed extract prepared in Example 1 to prepare a soaking solution.

[0039] Table 1

[0040] Experimental Group <![CDATA[CaCl2 concentration]]> Diethylaminoethanol hexanoate concentration 1 15mmol / L 30mg / L 2 20mmol / L 35mg / L 3 25mmol / L 40mg / L

[0041] (2) Soak 150 watercress seeds in 75% alcohol by volume for 10 minutes.

[0042] (3) The alcohol-soaked mustard seeds were divided into three groups, and each group was soaked in the soaking solution prepared in step (1) for 10 hours. At the sixth hour of soaking, the seeds were ultrasonically treated at 80 kHz for 1.5 hours.

[0043] (4) Planting the seeds soaked in step (3) in soil and culturing them for 15 days.

[0044] Experimental Example 1

[0045] According to Experimental Group 2 of Example 2, the ultrasonic time was set to 0.5h, 1h, 1.5h, 2h and 2.5h respectively, and other operations were the same as Experimental Group 2 of Example 2, and the seed germination rate and the seedling index after seedling formation were statistically analyzed, and the results are shown in Table 2. As can be seen from Table 2, the ultrasonic time of 1-2h can increase the germination rate of seeds on the third day to more than 90%, and the germination rate is reduced when the ultrasonic time is too high, and the seedling index is greatly reduced, which may be due to the fact that the ultrasonic time is too long, which causes a certain degree of damage to the seeds and affects the growth and development of the seedling stage.

[0046] Table 2

[0047] Ultrasound time Germination rate on the 3rd day, % Germination rate on the 5th day, % Seedling index 0.5h 86.7％ 88.0％ 0.61 1h 90.7％ 92.7％ 0.65 1.5h 97.3％ 98.7％ 0.75 2h 93.3％ 94.7％ 0.71 2.5h 89.3％ 90.0％ 0.53

[0048] Comparative Example 1

[0049] The same as Experimental Group 2 of Example 2, except that the immersion liquid was replaced with the same volume of distilled water.

[0050] Comparative Example 2

[0051] The same as Experimental Group 2 of Example 2, except that no ultrasonic treatment was performed.

[0052] Comparative Example 3

[0053] The same as Experimental Group 2 of Example 2, except that no CaCl2 was added to the soaking solution.

[0054] Comparative Example 4

[0055] The same as Experimental Group 2 of Example 2, except that diethylaminoethanol caproate was not added to the soaking solution.

[0056] Comparative Example 5

[0057] The same as Experimental Group 2 of Example 2, except that the mangrove seed extract was replaced with an equal volume of distilled water, and CaCl2 and diethylaminoethanol hexanoate were added to the distilled water to prepare a soaking solution.

[0058] The seed germination rate and seedling index after seedling formation of each experimental group in Example 2 and comparative examples 1-5 were statistically analyzed, and the results are shown in Table 3. The results of germination of experimental group 2 in Example 2 for 5 days are as follows Figure 1 shown.

[0059] Table 3

[0060]

[0061] It can be seen from Table 3 that soaking seeds in the extract of mangrove seeds germinated under the conditions of salt concentration of 0.2% and pH of 8 is beneficial to improving the seed germination rate and seedling index of water mustard in mild saline-alkali land; adding CaCl2 and diethylaminoethanol caproate to the soaking solution can further improve the seed germination rate and seedling index; ultrasonic treatment during the seed soaking process can assist in enhancing the effect of the mangrove seed extract and further improve the seed germination rate.

[0062] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for improving the vitality of water mustard seeds, characterized in that: The following steps are involved: (1) Disinfecting water mustard seeds; (2) soaking the sterilized water mustard seeds in a soaking solution, and performing ultrasonic treatment during the soaking process; The soaking solution is prepared by adding CaCl2 and diethylaminoethanol hexanoate to the mangrove seed extract; The extraction method of the mangrove seed extract is as follows: soaking the mangrove seeds for germination under the conditions of a salt content of 0.2%-0.3% and a pH of 8-8.5, then crushing the germinated seeds, and then adding distilled water for leaching to obtain the mangrove seed extract; the mass volume ratio of the germinated seeds to the distilled water is 1g:20mL; The concentration of CaCl2 in the soaking solution is 15-25mmol / L; The concentration of diethylaminoethanol caproate in the soaking solution is 30-40 mg / L.

2. The method for improving the vigor of watercress seeds according to claim 1, characterized in that: In step (2), the sterilized water mustard seeds are subjected to ultrasonic treatment in the 6th hour of being soaked in the soaking liquid.

3. The method for improving the vigor of Brassica juncea seeds according to claim 2, characterized in that: The frequency of the ultrasound is 80 kHz.

4. The method for improving the vigor of watercress seeds according to claim 2, characterized in that: The ultrasonic time is 1-2h.

5. The method for improving the vigor of watercress seeds according to claim 1, characterized in that: The seed soaking time is 10-16h.

6. The method for improving the vigor of Brassica juncea seeds according to claim 1, characterized in that: The disinfection treatment is as follows: soaking the water mustard seeds in 75% alcohol by volume for 10 minutes.

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