An anti-aging plant composition and its preparation method and application

Abstract

The present invention discloses an anti-aging plant composition, which comprises a herb of motherwort extract, a herb of millettia reticulata extract, a herb of ginseng extract and a herb of notoginseng extract. The present invention also discloses a preparation method and application of the anti-aging plant composition. The anti-aging plant composition of the present invention can promote the regeneration of stratum corneum, increase the synthesis of type I collagen, reduce the level of inflammation, inhibit the level of antioxidant, protect the skin and resist aging.

Description

Technical Field

[0001] The invention belongs to the field of skin care products, and specifically relates to an anti-aging plant composition and a preparation method and application thereof. Background Art

[0002] Aging is a natural phenomenon that humans must experience in their lives. It is a very complex physiological process, which is usually manifested as dry and dehydrated skin, loss of collagen, increased expression lines, sagging skin, etc. With the progress and development of society, people's living standards are getting higher and higher, skin care products are becoming more and more popular, and the demand for anti-aging effects of skin care products is particularly strong.

[0003] Currently, most skin care products on the market have only a single anti-aging effect, and the efficacy of the ingredients has a single pathway of action, which cannot achieve all-round skin care. At the same time, there are very few products that can exert anti-aging effects from multiple dimensions.

[0004] Most of the existing anti-aging cosmetic raw materials have a positive effect on promoting the proliferation of keratinocytes or the expression of collagen in fibroblasts, but there is still a lack of cosmetic compound raw materials that can exert anti-aging effects from multiple dimensions. Therefore, it is of great significance to develop an anti-aging product that acts on all pathways. Summary of the invention

[0005] Based on this, the purpose of the present invention is to provide a plant composition with good skin care and anti-aging effects in order to solve the above technical problems.

[0006] An anti-aging plant composition comprises motherwort extract, millettia spatholobi extract, ginseng extract and notoginseng extract.

[0007] More preferably, the volume ratio of Leonurus japonicus extract, Millettia spatholobi extract, ginseng extract and Panax notoginseng extract is 1-3:1-3:1-3:1-3, more preferably 1:1:2:2.

[0008] The anti-aging plant composition of the present invention can promote the regeneration of stratum corneum, increase collagen synthesis, reduce inflammation level, inhibit antioxidant level, protect skin and resist aging.

[0009] Further preferably, the motherwort extract is prepared by the following steps:

[0010] (1) crushing the motherwort to obtain motherwort powder;

[0011] (2) adding 95% v / v ethanol in an amount 15 times the weight of Leonurus japonicus as the extraction solvent, performing ultrasonic extraction for 30 min, performing extraction twice, and combining the extracts;

[0012] (3) centrifuging the extract at 4°C / 6000 rpm for 15 min, filtering the supernatant to obtain a filtrate;

[0013] (4) Concentrating the filtrate to remove ethanol and concentrating it to 5% of the initial volume, adding water and continuing to concentrate it to 10% of the initial volume, and filtering the concentrate with a 0.45 μm filter membrane;

[0014] (5) The filtrate is heated to 50° C.-55° C., stirred at a speed of 300-800 rpm, and a flocculant accounting for 10% of the volume of the filtrate is added, and the heating and stirring are continued for 20 minutes, and the filtrate is allowed to settle and filtered. The filtrate is filtered through a 0.22 um filter membrane to obtain a filtrate of Leonurus japonicus;

[0015] (6) 50 parts by weight of glycerin, 48.4 parts by weight of Leonurus japonicus filtrate, 1.6 parts by weight of p-hydroxyacetophenone and a mixed solution of 1,2-hexanediol were stirred uniformly to obtain the Leonurus japonicus extract.

[0016] Further preferably, the Millettia spatholobi extract is prepared by the following steps:

[0017] (1) crushing Millettia spatholobi to obtain Millettia spatholobi powder;

[0018] (2) adding 70% v / v ethanol in an amount 15 times the weight of Millettia reticulata as an extraction solvent, performing ultrasonic extraction for 30 min, performing extraction twice, and combining the extracts;

[0019] (3) centrifuging the extract at 4°C / 6000 rpm for 15 min, filtering the supernatant to obtain a filtrate;

[0020] (4) Concentrating the filtrate to remove ethanol and concentrating it to 10% of the initial volume, and filtering the concentrate with a 0.45 μm filter membrane;

[0021] (5) The filtrate is heated to 50° C.-55° C., stirred at a speed of 300-800 rpm, and a flocculant accounting for 10% of the volume of the filtrate is added, and the heating and stirring are continued for 20 min, and the filtrate is allowed to settle and filtered. The filtrate is filtered through a 0.22 μm filter membrane to obtain a Millettia spatholobi filtrate;

[0022] (6) 50 parts by weight of glycerin, 48.4 parts by weight of Millettia spatholobi filtrate, 1.6 parts by weight of p-hydroxyacetophenone and a mixed solution of 1,2-hexanediol were stirred uniformly to obtain the Millettia spatholobi extract.

[0023] Further preferably, the ginseng extract is prepared by the following steps:

[0024] (1) crushing ginseng to obtain ginseng particles;

[0025] (2) adding a 10% wt glycerol aqueous solution having a weight that is 3.5 times the weight of ginseng as an extraction solvent, squeezing the juice for extraction, filtering to remove the residue, centrifuging the filtrate at 4°C / 6000 rpm for 15 min, filtering the supernatant to obtain a filtrate;

[0026] (3) concentrating the filtrate to obtain a ginseng extract;

[0027] (4) 50 parts by weight of glycerin, 18.5 parts by weight of water, 30 parts by weight of ginseng extract, 0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol were stirred uniformly to obtain the ginseng extract.

[0028] Further preferably, the Panax notoginseng extract is prepared by the following steps:

[0029] (1) crushing Panax notoginseng to obtain Panax notoginseng granules;

[0030] (2) adding a 10% wt glycerol aqueous solution whose weight is 3.5 times the weight of Panax notoginseng as an extraction solvent, squeezing out the juice, filtering to remove the residue, centrifuging the filtrate at 4°C / 6000rpm for 15min, filtering the supernatant to obtain a filtrate;

[0031] (3) concentrating the filtrate to obtain a Panax notoginseng extract;

[0032] (4) 50 parts by weight of glycerin, 18.5 parts by weight of water, 30 parts by weight of Panax notoginseng extract, 0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol were stirred uniformly to obtain the Panax notoginseng extract.

[0033] Further preferably, in the mixed solution of p-hydroxyacetophenone and 1,2-hexanediol, the weight ratio of p-hydroxyacetophenone to 1,2-hexanediol is 1:1.

[0034] Further preferably, the flocculant comprises, by weight, 1 part of chitosan, 99 parts of water and 1 part of acetic acid.

[0035] The present invention further provides a skin care product, which contains the anti-aging plant composition of the present invention as an active ingredient.

[0036] Further preferably, the skin care product contains 0.1%-2% of the anti-aging plant composition by weight.

[0037] The present invention further provides a method for preparing the anti-aging plant composition, which comprises the following steps: mixing motherwort extract, millettia spatholobi extract, ginseng extract and notoginseng extract, and stirring evenly.

[0038] The present invention further provides application of the anti-aging plant composition in promoting proliferation of human keratinocytes.

[0039] The present invention further provides application of the anti-aging plant composition in promoting collagen synthesis.

[0040] The present invention further provides the use of the anti-aging plant composition in reducing inflammation levels.

[0041] The present invention further provides the use of the anti-aging plant composition in reducing lipid oxidation levels.

[0042] Compared with the prior art, the present invention has optimized the extraction process, including extraction solvent, extraction time, ultrasound, extraction temperature, extraction times, etc., to extract four traditional Chinese medicines, namely, Leonurus japonicus, Millettia reticulata, ginseng, and Panax notoginseng, to obtain an anti-aging plant composition, which can promote the regeneration of stratum corneum, increase the synthesis of type I collagen, reduce the level of inflammation, inhibit the level of antioxidants, protect the skin, and resist aging.

[0043] For better understanding and implementation, the present invention is described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 The results show that the anti-aging plant composition of the present invention has a promoting effect on the proliferation of keratinocytes.

[0045] Figure 2 It is shown that the anti-aging plant composition of the present invention reduces the expression level of TNF-α, an inflammatory factor in macrophages.

[0046] Figure 3 It is shown that the anti-aging plant composition of the present invention reduces the expression level of macrophage inflammatory factor IL-6.

[0047] Figure 4 It is shown that the anti-aging botanical composition of the present invention reduces the lipid oxidation level of keratinocytes.

[0048] Figure 5 It is shown that the anti-aging plant composition of the present invention promotes collagen synthesis in fibroblasts. DETAILED DESCRIPTION

[0049] The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present disclosure. Instead, they are merely examples of embodiments consistent with some aspects of the present disclosure.

[0050] The terms used in this disclosure are only for the purpose of describing specific embodiments and are not intended to limit the disclosure. The singular forms of "a", "the" and "the" used in this disclosure are also intended to include plural forms, unless the context clearly indicates other meanings. It should also be understood that the term "and / or" used herein refers to and includes any or all possible combinations of one or more associated listed items.

[0051] Example 1 - Preparation of Leonurus japonicus Extract

[0052] 1. Grind the motherwort to obtain motherwort powder;

[0053] 2. Weigh the motherwort powder, add 95% v / v ethanol as the extraction solvent with a weight of 15 times the weight of the motherwort, perform ultrasonic extraction for 30 minutes, extract twice, and combine the extracts;

[0054] 3. Centrifuge the extract in a floor-standing high-speed refrigerated centrifuge at 4°C / 6000rpm for 15 minutes, filter the supernatant with medium-speed filter paper to obtain the filtrate;

[0055] 4. Use a rotary evaporator to concentrate the filtrate under reduced pressure to remove ethanol and concentrate it to 5% of the initial volume. Add a small amount of water and continue to concentrate it to 10% of the initial volume. Filter the concentrate with a 0.45um filter membrane;

[0056] 5. Heat the filtrate to 50-55°C, stir at 300-800rpm, add 10% of the volume of the filtrate with a flocculant (dissolve 1 part of chitosan by weight in 99 parts of pure water, stir with a magnetic stirrer, add 1 part of acetic acid by weight, mix well, and obtain a flocculant), continue heating and stirring for 20 minutes, let stand and settle, filter with medium-speed filter paper, and filter the filtrate with a 0.22um filter membrane. After the membrane filtration equipment is fully cleaned with ultrapure water, adjust the pressure valve, and the pressure value of the pressure gauge during the process does not exceed 0.4MPa to obtain the motherwort filtrate. The function of the flocculant is to precipitate the water-insoluble components in the extract and filter them out. The flocculation effect is mainly to add a polymer flocculant to the system. The polymer flocculant forms hydrogen bonds or ion pairs with the particles through its own polar groups or ionic groups, and adsorbs on the surface of the particles due to the van der Waals force, bridges between the particles to form a large flocculent precipitate and separates from the aqueous solution. The characteristics of flocculation are small amount of flocculant, fast volume increase, fast floc formation and high flocculation efficiency.

[0057] 6. Mix 50 parts by weight of glycerin, 48.4 parts by weight of motherwort filtrate, and 1.6 parts by weight of a mixed solution of p-hydroxyacetophenone and 1,2-hexanediol (the weight ratio of p-hydroxyacetophenone to 1,2-hexanediol is 1:1) to obtain a motherwort extract.

[0058] Example 2 - Preparation of Millettia repens extract

[0059] 1. Crush the Millettia spatholobi to obtain Millettia spatholobi powder;

[0060] 2. Weigh the Millettia spatholobi powder, add 70% v / v ethanol as an extraction solvent with a weight 15 times the weight of Millettia spatholobi, perform ultrasonic extraction for 30 min, extract twice, and combine the extracts;

[0061] 3. Centrifuge the extract in a floor-standing high-speed refrigerated centrifuge at 4°C / 6000rpm for 15 minutes, filter the supernatant with medium-speed filter paper to obtain the filtrate;

[0062] 4. Use a rotary evaporator to concentrate the filtrate under reduced pressure to remove ethanol and concentrate it to 10% of the initial volume. Filter the concentrate with a 0.45um filter membrane;

[0063] 5. Heat the filtrate to 50-55°C, stir at 300-800rpm, add 10% of the volume of the filtrate with a flocculant (dissolve 1 part of chitosan by weight in 99 parts of pure water, stir with a magnetic stirrer, add 1 part of acetic acid by weight, mix well, and obtain a flocculant), continue heating and stirring for 20 minutes, let stand and settle, filter with medium-speed filter paper, and filter the filtrate with a 0.22um filter membrane. After the membrane filtration equipment is fully cleaned with ultrapure water, adjust the pressure valve, and the pressure value of the pressure gauge during the process does not exceed 0.4MPa to obtain the Millettia reticulata filtrate;

[0064] 6. Mix 50 parts by weight of glycerin, 48.4 parts by weight of Millettia spatholobi filtrate, and 1.6 parts by weight of a mixed solution of p-hydroxyacetophenone and 1,2-hexanediol (the weight ratio of p-hydroxyacetophenone to 1,2-hexanediol is 1:1) to obtain a Millettia spatholobi extract.

[0065] Example 3 - Preparation of ginseng extract

[0066] 1. Crush the ginseng to obtain ginseng crumbs.

[0067] 2. Weigh the crushed ginseng, add 10%wt glycerol aqueous solution (10 parts by weight of glycerol + 90 parts by weight of pure water) with a weight of 3.5 times the weight of ginseng as the extraction solvent, squeeze the juice for extraction, filter the juice obtained by squeezing with a filter bag to remove the residue, centrifuge the filtrate in a floor-standing high-speed refrigerated centrifuge at 4℃ / 6000rpm for 15min, and filter the supernatant with medium-speed filter paper. After the membrane filtration equipment is fully washed with ultrapure water, the filtrate is concentrated at the fully opened reflux end. During the concentration process, the pressure gauge pressure value does not exceed 0.4MPa to obtain the filtrate.

[0068] 3. Concentrate the filtrate to obtain ginseng extract.

[0069] 4. Mix 50 parts by weight of glycerin, 18.5 parts by weight of water, 30 parts by weight of ginseng extract, 0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol (0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol are mixed, stirred and heated to 70°C to dissolve for later use) and stir evenly to obtain the ginseng extract.

[0070] Example 4- Preparation of Panax notoginseng extract

[0071] 1. Crush the Panax notoginseng to obtain Panax notoginseng granules.

[0072] 2. Weigh the crushed Panax notoginseng, add 10%wt glycerol aqueous solution (10 parts by weight of glycerol + 90 parts by weight of pure water) which is 3.5 times the weight of Panax notoginseng as the extraction solvent, extract the juice, filter the juice obtained by juicing with a filter bag to remove the residue, centrifuge the filtrate in a floor-standing high-speed refrigerated centrifuge at 4℃ / 6000rpm for 15min, and filter the supernatant with medium-speed filter paper. After the membrane filtration equipment is fully washed with ultrapure water, the filtrate is concentrated at the fully opened reflux end. During the concentration process, the pressure gauge pressure value does not exceed 0.4MPa to obtain the filtrate.

[0073] 3. Concentrate the filtrate to obtain Panax notoginseng extract.

[0074] 4. Mix 50 parts by weight of glycerin, 18.5 parts by weight of water, 30 parts by weight of Panax notoginseng extract, 0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol (0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol are mixed, stirred and heated to 70°C to dissolve for later use) and stir evenly to obtain the Panax notoginseng extract.

[0075] The Leonurus japonicus extract, Millettia spatholobi extract, Panax ginseng extract and Panax notoginseng extract obtained in the above Examples 1-4 are prepared in a volume ratio of 1:1:2:2, mixed and stirred evenly to obtain the anti-aging plant composition of the present invention.

[0076] The anti-aging plant composition of the present invention has a yellow-brown solution appearance and is suitable for use in water, essence, eye mask, facial mask and other common skin care product formulations, and the recommended usage is 0.1%-2% (by weight).

[0077] Example 5 - Proliferation promotion experiment of human keratinocytes HaCaT (CCK-8 method)

[0078] 1. Main instruments

[0079] Incubator, clean bench, microplate reader, pipette, etc.

[0080] 2. Main reagents

[0081] DMEM medium, 0.25% trypsin, PBS, CCK-8.

[0082] 3. Experimental steps

[0083] (1) Keratinocyte seeding plate. Keratinocytes were plated at 100 μL per well at 2.0×10 3 cells and cultured for 12 h.

[0084] (2) The supernatant was discarded, and hexapeptide-9 (100, 50, 25, 10, 5, 1, 0.5, 0.1 μg / mL), NMN (nicotinamide mononucleotide) (100, 50, 25, 10, 5, 1, 0.5, 0.1 μg / mL), and the anti-aging plant composition of the present invention (100, 50, 25, 10, 5, 1, 0.5, 0.1 mg / mL) pre-diluted with DMEM medium were added and cultured for 24 h.

[0085] (3) Discard the supernatant, add 100uL of complete culture medium containing 10% CCK-8 reagent to each well, incubate for 2.5h, and detect with an enzyme-labeled instrument at 450nm.

[0086] The experimental results are as follows Figure 1 As shown, the anti-aging plant composition of the present invention still has a significant proliferation-promoting effect on keratinocytes at a concentration below 5 mg / mL, and its effect intensity is stronger than that of the positive control hexapeptide-9 and NMN.

[0087] Example 6-Reducing the expression level of macrophage inflammatory factor TNF-α

[0088] 1. Main instruments

[0089] Incubator, clean bench, microplate reader, pipette, etc.

[0090] 2. Main reagents

[0091] DMEM medium, 0.25% trypsin, PBS, TNF-α kit.

[0092] 3. Experimental steps

[0093] (1) Use a 6-well plate and seed the macrophage RAW264.7 cells. 1.0×10 macrophage RAW264.7 cells are plated at 1 mL per well. 4 cells and cultured for 12 h.

[0094] (2) The supernatant was discarded, and 10 μg / mL lipopolysaccharide (LPS) diluted in culture medium was added to both the model group and the test group to induce an inflammatory cell model, while only complete culture medium was added to the blank control group.

[0095] (3) The supernatant was discarded, and 10 mg / mL of dexamethasone diluted in culture medium was added to the model group (DXMS), and the anti-aging plant composition of the present invention (100 (AAS-H), 50 (AAS-M), 25 (AAS-L) mg / mL) was added to the test group. The blank control group (Control) was not treated and cultured for 24 hours.

[0096] (4) Aspirate the culture supernatant, follow the instructions of the TNF-α detection kit, and detect with an ELISA reader.

[0097] The experimental results are as follows Figure 2 As shown, the anti-aging plant composition of the present invention effectively reduces the expression level of tumor necrosis factor in RAW264.7 cells, and the inflammation-reducing effect of 100 mg / mL AAS is 28.4% stronger than that of 10 mg / mL dexamethasone.

[0098] Example 7-Reducing the expression level of macrophage inflammatory factor IL-6

[0099] 1. Main instruments

[0100] Incubator, clean bench, microplate reader, pipette, etc.

[0101] 2. Main reagents

[0102] DMEM medium, 0.25% trypsin, PBS, IL-6 kit.

[0103] 3. Experimental steps

[0104] (1) Use a 6-well plate and seed the macrophage RAW264.7 cells. 1.0×10 macrophage RAW264.7 cells are plated at 1 mL per well. 4 cells and cultured for 12 h.

[0105] (2) The supernatant was discarded, and 10 μg / mL lipopolysaccharide (LPS) diluted in culture medium was added to both the model group and the test group to induce an inflammatory cell model, while the blank control group was only fed with complete culture medium.

[0106] (3) The supernatant was discarded, and 10 mg / mL of dexamethasone diluted in culture medium was added to the model group (DXMS), and the anti-aging plant composition of the present invention (100 (AAS-H), 50 (AAS-M), 25 (AAS-L) mg / mL) was added to the test group. The blank control group (Control) was not treated and cultured for 24 hours.

[0107] (4) Aspirate the culture supernatant, follow the instructions of the IL-6 detection kit, and detect with an ELISA reader.

[0108] The experimental results are as follows Figure 3 As shown, the anti-aging plant composition of the present invention effectively reduces the expression level of interleukin-6 in RAW264.7 cells, and the inflammation-reducing effect of 100 mg / mL AAS is 63.9% stronger than that of 10 mg / mL dexamethasone.

[0109] Example 8 - Reduction of lipid oxidation levels (MDA)

[0110] 1. Main instruments

[0111] Incubator, clean bench, microplate reader, pipette, etc.

[0112] 2. Main reagents

[0113] DMEM medium, 0.25% trypsin, PBS, MDA kit.

[0114] 3. Experimental steps

[0115] (1) Use a 6-well plate and seed the plate with keratinocytes. Keratinocytes are plated at 1 mL per well at a concentration of 1.0 × 10 4 cells and cultured for 12 h.

[0116] (2) The supernatant was discarded, and 10 mg / mL of vitamin C diluted in culture medium was added to the model group (Vc), 100 mg / mL of the anti-aging plant composition of the present invention was added to the test group (AAS), and the blank control group (Control) was not treated and cultured for 24 hours.

[0117] (3) Aspirate the culture supernatant, follow the instructions of the MDA detection kit, and perform detection with an ELISA reader.

[0118] The experimental results are as follows Figure 4 As shown, the anti-aging plant composition of the present invention can effectively reduce the lipid oxidation level of keratinocytes and has a certain antioxidant effect. The antioxidant effect of 100 mg / mL of the anti-aging plant composition of the present invention is 17.7% stronger than that of vitamin C.

[0119] Example 9 - Promoting collagen synthesis

[0120] 1. Main instruments

[0121] Incubator, clean bench, microplate reader, pipette, etc.

[0122] 2. Main reagents

[0123] DMEM medium, 0.25% trypsin, PBS, type I collagen detection kit.

[0124] 3. Experimental steps

[0125] (1) Use a 6-well plate to seed fibroblasts. 1.0×10 fibroblasts are plated at 1 mL per well. 4 cells and cultured for 12 h.

[0126] (2) The supernatant was discarded, and the blank control group (Control) was not treated. The anti-aging plant composition of the present invention (100 (AAS-H), 50 (AAS-M), 25 (AAS-L) mg / mL) was added to the test group, and the blank control group was not treated, and the culture was continued for 24 hours.

[0127] (3) Aspirate the culture supernatant and perform the assay according to the instructions of the type I collagen assay kit and perform the assay using an ELISA reader.

[0128] The experimental results are as follows Figure 5 As shown, compared with the blank control group, the anti-aging plant composition of the present invention has the effect of significantly promoting the collagen synthesis of fibroblasts. Relatively speaking, the collagen expression amount at a concentration of 50 mg / mL increased by 21.1%, which has the best effect.

[0129] It can be seen that the anti-aging plant composition of the present invention has a multi-dimensional effect of protecting the skin and resisting skin aging. Through various cell efficacy evaluation experiments, including human keratinocytes HaCaT, human fibroblasts HSF, macrophages RAW264.7 and various ELISA experiments (including TNF-α, IL-6, MDA, COL1α1, etc.), it is proved that it has the effects of promoting stratum corneum regeneration, increasing type I collagen synthesis, reducing inflammation levels, inhibiting antioxidant levels, protecting the skin, resisting aging and the like.

[0130] The above-mentioned embodiments only express several implementation methods of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

[0131] References:

[0132] [1]Jintao Gao,Fangru Chen,Huanan Fang,et al.Daphnetin inhibitsproliferation and inflammatory response in human HaCaT keratinocytes andameliorates imiquimod-induced psoriasis-like skin lesion in mice[J].BiolRes.2020,53(1):48.doi:10.1186 / s40659-020-00316-0.

[0133] [2]Christopher S Smillie, Moshe Biton, Jose Ordovas-Montanes, et al.Intra-and Inter-cellular rewiring of the human colon during ulcerative colitis[J].

[0134] [3]Hua Qing,Reina Desrollaux,Kavita Israni-Winger,et al.Origin andFunction of Stress-Induced IL-6in Murine Models[J].Cell.2020,182(2):372-387.e14.doi:10.1016 / j.cell.2020.05.054.

[0135] [4]Retno Sri Iswari,Muchamad Dafip,Eling Purwantoyo.Malondialdehyde(MDA) Production in Atherosclerosis Supplemented with Steamed Tomato[J].Pak JBiol Sci.2021,24(3):319-325.doi:10.3923 / pjbs.2021.319.325.

[0136] [5]Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,et al.Induction ofpluripotent stem cells from adult human fibroblasts by defined factors[J].Cell,2007,131,861-872.

Claims

1. An anti-aging plant composition, characterized in that: The invention is composed of motherwort extract, millettia spatholobi extract, ginseng extract and notoginseng extract in a volume ratio of 1-3:1-3:1-3:1-3; The motherwort extract is prepared by the following steps: (1) crushing the motherwort to obtain motherwort powder; (2) Add 95% v / v ethanol in an amount 15 times the weight of Leonurus japonicus as the extraction solvent, perform ultrasonic extraction for 30 min, perform extraction twice, and combine the extracts; (3) Centrifuge the extract at 4°C / 6000 rpm for 15 min, filter the supernatant to obtain the filtrate; (4) Concentrate the filtrate to remove ethanol and concentrate it to 5% of the initial volume. Add water and continue to concentrate it to 10% of the initial volume. Filter the concentrate with a 0.45 μm filter membrane. (5) The filtrate is heated to 50°C-55°C, stirred at a speed of 300-800 rpm, and a flocculant accounting for 10% of the volume of the filtrate is added. The heating and stirring are continued for 20 minutes, and the filtrate is allowed to settle. The filtrate is filtered and filtered through a 0.22 um filter membrane to obtain a filtrate of Leonurus japonicus. (6) 50 parts by weight of glycerin, 48.4 parts by weight of motherwort filtrate, 1.6 parts by weight of p-hydroxyacetophenone and a mixed solution of 1,2-hexanediol were stirred uniformly to obtain the motherwort extract; The Millettia spatholobi extract is prepared by the following steps: (1) crushing Millettia spatholobi to obtain Millettia spatholobi powder; (2) adding 70% v / v ethanol in an amount 15 times the weight of Millettia reticulata as the extraction solvent, performing ultrasonic extraction for 30 min, performing extraction twice, and combining the extracts; (3) Centrifuge the extract at 4°C / 6000 rpm for 15 min, filter the supernatant to obtain the filtrate; (4) Concentrate the filtrate to remove ethanol and concentrate it to 10% of the initial volume. Filter the concentrate with a 0.45 μm filter membrane; (5) The filtrate is heated to 50°C-55°C, stirred at a speed of 300-800 rpm, and a flocculant accounting for 10% of the volume of the filtrate is added, and the heating and stirring are continued for 20 minutes, and the filtrate is allowed to settle and filtered. The filtrate is filtered through a 0.22 um filter membrane to obtain a Millettia spatholobi filtrate; (6) 50 parts by weight of glycerin, 48.4 parts by weight of Millettia spatholobi filtrate, 1.6 parts by weight of p-hydroxyacetophenone and a mixed solution of 1,2-hexanediol were stirred uniformly to obtain the Millettia spatholobi extract; The ginseng extract is prepared by the following steps: (1) crushing ginseng to obtain ginseng crumbs; (2) adding a 10%wt glycerol aqueous solution with a weight of 3.5 times the weight of ginseng as an extraction solvent, squeezing the juice for extraction, filtering to remove the residue, centrifuging the filtrate at 4°C / 6000 rpm for 15 min, filtering the supernatant to obtain a filtrate; (3) concentrating the filtrate to obtain a ginseng extract; (4) 50 parts by weight of glycerin, 18.5 parts by weight of water, 30 parts by weight of ginseng extract, 0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol were uniformly stirred to obtain the ginseng extract; The Panax notoginseng extract is prepared by the following steps: (1) crushing the Panax notoginseng to obtain Panax notoginseng granules; (2) adding a 10%wt glycerol aqueous solution whose weight is 3.5 times the weight of Panax notoginseng as an extraction solvent, squeezing out the juice, filtering to remove the residue, centrifuging the filtrate at 4°C / 6000 rpm for 15 min, filtering the supernatant to obtain a filtrate; (3) concentrating the filtrate to obtain a Panax notoginseng extract; (4) 50 parts by weight of glycerin, 18.5 parts by weight of water, 30 parts by weight of Panax notoginseng extract, 0.5 parts by weight of p-hydroxyacetophenone and 1.0 parts by weight of 1,2-hexanediol were stirred uniformly to obtain the Panax notoginseng extract.

2. The anti-aging plant composition according to claim 1, characterized in that: In the mixed solution of p-hydroxyacetophenone and 1,2-hexanediol, the weight ratio of p-hydroxyacetophenone to 1,2-hexanediol is 1:

1.

3. A skin care product comprising the anti-aging plant composition according to claim 1 or 2 as an active ingredient.

4. The method for preparing the anti-aging plant composition according to claim 1 or 2, comprising the following steps: Mix the motherwort extract, Millettia spatholobi extract, ginseng extract and Panax notoginseng extract and stir well.

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